MC02g1219 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g1219
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description26S proteasome non-ATPase regulatory subunit 11 homolog
LocationMC02: 11031466 .. 11032722 (+)
RNA-Seq ExpressionMC02g1219
SyntenyMC02g1219
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGATGTCCTACCTGCCAGCGACCATGGATACACTTATTCTGGCTTCAGAAAACGATAGCCCATCTGAAGCAATTTCAATCCTTTACCGCGTACTTGCAAACCCTTCATCTTCCCCTGAGGCCCTAAATATAAAAGAGCAGGCCATAATAGATATATGCTATATTTTCAACGACACTGATCGAGCCGAGGATCTTCGAAACCTTGTCACTCAACTAACACCATTATTCAGCTTGATATCCAAAGCAAAGACGGCTAGAATTGTTCGTGACATCATTGATGTTATAGGAGATATCACAGGCACATCTGAGTTGCAAATTTCTCTATGCAAGGAACTGGTGCAGTGGACACGTGCGGAGAACCGAACTTCCCTTCGGTATTGGGTCGAGGCCAGGCTTGCTGCTCTACTGATTGAAGAAAAGGACTACTCAGATGCCTTGAATCTCCTTGCAGAGTTGATCAAGGAAGTGGGAAGAATGGAGGACAAGCTTCTCCTTATAGAGATAAACTTGTTGGAGAGCAAGATTTACTTTTGTCTAAGGAATCTTCCCATGGCAAATGTGGCACTTTATGCTGCAAGAATAACTGCTCAATCCATATATGTGCCTATACACCAGCAGGGCGCCATTGATTTACAGAGTGGCATCCTCCATGCGGAGGAGAAGGATTATTGTGCTGCTTTCCACAGTTTCGCTGTAGCTCTTACAGCTTTCGACCTTTTCGAAGATCCCCGTGCTGTGATTTGCCTCAAATATTTGTTGTTGTGTGTAATAATGTGTGATCAGTCGTATCTTGTGCAGTTCGCAATCTCGCGTTATGGGGAGTGTTATGATAAGGAGATTGAGATAAATGCCATGAAAGCAGTGGCTTATGCTACTCATTCCAAGCGTTGTTCTATAAAGCTCTTCAAGGATGCACTTAGAGATTTTAGAGCTCAACTACAGGAAGATCTACTTGTCCACCTGCAGCTCTCTTCCCTCTACAATACTTTGTTTGAGAAAAACCTGTATCGGTTGATCGAACCGTTCTCGAGGGTCGAGATTGTCCATGTTGCTAATCTGATTGAGCTGCCTGTCGATCTGGTAGAAAGTAAGTTATCTCAGATGATTTTGGATAAGAAATTAACAGGAAATTTGGATCAAGGTTGCCTCATCATTTTTGATGATCCAAAAATAGATGGAATATATGCAGCAACTCTTGAAACCATCATAAACATTGGCAAGGTTGTGGATAGCCTTTATGTAAGGTCAACAAAG

mRNA sequence

ATGTCGATGTCCTACCTGCCAGCGACCATGGATACACTTATTCTGGCTTCAGAAAACGATAGCCCATCTGAAGCAATTTCAATCCTTTACCGCGTACTTGCAAACCCTTCATCTTCCCCTGAGGCCCTAAATATAAAAGAGCAGGCCATAATAGATATATGCTATATTTTCAACGACACTGATCGAGCCGAGGATCTTCGAAACCTTGTCACTCAACTAACACCATTATTCAGCTTGATATCCAAAGCAAAGACGGCTAGAATTGTTCGTGACATCATTGATGTTATAGGAGATATCACAGGCACATCTGAGTTGCAAATTTCTCTATGCAAGGAACTGGTGCAGTGGACACGTGCGGAGAACCGAACTTCCCTTCGGTATTGGGTCGAGGCCAGGCTTGCTGCTCTACTGATTGAAGAAAAGGACTACTCAGATGCCTTGAATCTCCTTGCAGAGTTGATCAAGGAAGTGGGAAGAATGGAGGACAAGCTTCTCCTTATAGAGATAAACTTGTTGGAGAGCAAGATTTACTTTTGTCTAAGGAATCTTCCCATGGCAAATGTGGCACTTTATGCTGCAAGAATAACTGCTCAATCCATATATGTGCCTATACACCAGCAGGGCGCCATTGATTTACAGAGTGGCATCCTCCATGCGGAGGAGAAGGATTATTGTGCTGCTTTCCACAGTTTCGCTGTAGCTCTTACAGCTTTCGACCTTTTCGAAGATCCCCGTGCTGTGATTTGCCTCAAATATTTGTTGTTGTGTGTAATAATGTGTGATCAGTCGTATCTTGTGCAGTTCGCAATCTCGCGTTATGGGGAGTGTTATGATAAGGAGATTGAGATAAATGCCATGAAAGCAGTGGCTTATGCTACTCATTCCAAGCGTTGTTCTATAAAGCTCTTCAAGGATGCACTTAGAGATTTTAGAGCTCAACTACAGGAAGATCTACTTGTCCACCTGCAGCTCTCTTCCCTCTACAATACTTTGTTTGAGAAAAACCTGTATCGGTTGATCGAACCGTTCTCGAGGGTCGAGATTGTCCATGTTGCTAATCTGATTGAGCTGCCTGTCGATCTGGTAGAAAGTAAGTTATCTCAGATGATTTTGGATAAGAAATTAACAGGAAATTTGGATCAAGGTTGCCTCATCATTTTTGATGATCCAAAAATAGATGGAATATATGCAGCAACTCTTGAAACCATCATAAACATTGGCAAGGTTGTGGATAGCCTTTATGTAAGGTCAACAAAG

Coding sequence (CDS)

ATGTCGATGTCCTACCTGCCAGCGACCATGGATACACTTATTCTGGCTTCAGAAAACGATAGCCCATCTGAAGCAATTTCAATCCTTTACCGCGTACTTGCAAACCCTTCATCTTCCCCTGAGGCCCTAAATATAAAAGAGCAGGCCATAATAGATATATGCTATATTTTCAACGACACTGATCGAGCCGAGGATCTTCGAAACCTTGTCACTCAACTAACACCATTATTCAGCTTGATATCCAAAGCAAAGACGGCTAGAATTGTTCGTGACATCATTGATGTTATAGGAGATATCACAGGCACATCTGAGTTGCAAATTTCTCTATGCAAGGAACTGGTGCAGTGGACACGTGCGGAGAACCGAACTTCCCTTCGGTATTGGGTCGAGGCCAGGCTTGCTGCTCTACTGATTGAAGAAAAGGACTACTCAGATGCCTTGAATCTCCTTGCAGAGTTGATCAAGGAAGTGGGAAGAATGGAGGACAAGCTTCTCCTTATAGAGATAAACTTGTTGGAGAGCAAGATTTACTTTTGTCTAAGGAATCTTCCCATGGCAAATGTGGCACTTTATGCTGCAAGAATAACTGCTCAATCCATATATGTGCCTATACACCAGCAGGGCGCCATTGATTTACAGAGTGGCATCCTCCATGCGGAGGAGAAGGATTATTGTGCTGCTTTCCACAGTTTCGCTGTAGCTCTTACAGCTTTCGACCTTTTCGAAGATCCCCGTGCTGTGATTTGCCTCAAATATTTGTTGTTGTGTGTAATAATGTGTGATCAGTCGTATCTTGTGCAGTTCGCAATCTCGCGTTATGGGGAGTGTTATGATAAGGAGATTGAGATAAATGCCATGAAAGCAGTGGCTTATGCTACTCATTCCAAGCGTTGTTCTATAAAGCTCTTCAAGGATGCACTTAGAGATTTTAGAGCTCAACTACAGGAAGATCTACTTGTCCACCTGCAGCTCTCTTCCCTCTACAATACTTTGTTTGAGAAAAACCTGTATCGGTTGATCGAACCGTTCTCGAGGGTCGAGATTGTCCATGTTGCTAATCTGATTGAGCTGCCTGTCGATCTGGTAGAAAGTAAGTTATCTCAGATGATTTTGGATAAGAAATTAACAGGAAATTTGGATCAAGGTTGCCTCATCATTTTTGATGATCCAAAAATAGATGGAATATATGCAGCAACTCTTGAAACCATCATAAACATTGGCAAGGTTGTGGATAGCCTTTATGTAAGGTCAACAAAG

Protein sequence

MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDTDRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAENRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCLRNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDLFEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSIKLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVDLVESKLSQMILDKKLTGNLDQGCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK
Homology
BLAST of MC02g1219 vs. ExPASy Swiss-Prot
Match: Q9LP45 (26S proteasome non-ATPase regulatory subunit 11 homolog OS=Arabidopsis thaliana OX=3702 GN=RPN6 PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 8.9e-128
Identity = 261/414 (63.04%), Postives = 316/414 (76.33%), Query Frame = 0

Query: 8   ATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDTDRAEDLR 67
           AT +T+ LA E +S SEAI+ILY+VL +PSSSPEA+ IKEQAI ++C    +  R EDLR
Sbjct: 6   ATTETISLALEANS-SEAITILYQVLEDPSSSPEAIRIKEQAITNLCDRLTEEKRGEDLR 65

Query: 68  NLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAENRTSLRY 127
            L+T+L P FSLI KAKTA+IVR IID +  I GT++LQI+LCKE+V+WTRAE RT LR 
Sbjct: 66  KLLTKLRPFFSLIPKAKTAKIVRGIIDAVAKIPGTTDLQITLCKEMVEWTRAEKRTFLRQ 125

Query: 128 WVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCLRNLPMAN 187
            VEARLAALL+E K+Y +AL LL+ L+KEV R++DKLLL++I+LLESK++F LRNLP A 
Sbjct: 126 RVEARLAALLMENKEYVEALALLSTLVKEVRRLDDKLLLVDIDLLESKLHFSLRNLPKAK 185

Query: 188 VALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDLFEDPRAV 247
            AL AAR  A +IYVP  QQG IDLQSGILHAEEKDY   +  F  A  +F+   DPRAV
Sbjct: 186 AALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTGYSYFFEAFESFNALGDPRAV 245

Query: 248 ICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSIKLFKDAL 307
             LKY+LLC IM  Q+  V   IS          +++AMKAVA A HSKR S+KLF++AL
Sbjct: 246 FSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGPDLDAMKAVADA-HSKR-SLKLFENAL 305

Query: 308 RDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVDLVESKLS 367
           RD++AQL++D +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LI LP+D VE KLS
Sbjct: 306 RDYKAQLEDDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIGLPLDHVEKKLS 365

Query: 368 QMILDKKLTGNLDQ--GCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 420
           QMILDKK  G LDQ  GCLIIF+DPK D IY+ATLETI N+GKVVDSLYVRS K
Sbjct: 366 QMILDKKFAGTLDQGAGCLIIFEDPKADAIYSATLETIANMGKVVDSLYVRSAK 416

BLAST of MC02g1219 vs. ExPASy Swiss-Prot
Match: F6XBL2 (26S proteasome non-ATPase regulatory subunit 11 OS=Xenopus tropicalis OX=8364 GN=psmd11 PE=3 SV=2)

HSP 1 Score: 305.8 bits (782), Expect = 7.3e-82
Identity = 177/385 (45.97%), Postives = 250/385 (64.94%), Query Frame = 0

Query: 38  SSPEALNIKEQAIIDICYIFNDTDRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIG 97
           S  EAL +KEQ+I+++  +   T +A +L  L+  + P  + ISKAK AR+VR ++D+  
Sbjct: 38  SDEEALRVKEQSILELGGLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVRSLLDLFL 97

Query: 98  DITGTSELQISLCKELVQWTRAENRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEV 157
           D+   +  ++ LC E ++W +AE RT LR  +EARL +L  + K Y +AL L ++L++E+
Sbjct: 98  DMEAATGQEVELCLECIEWAKAEKRTFLRQALEARLVSLYFDTKRYQEALQLGSQLLREL 157

Query: 158 GRMEDKLLLIEINLLESKIYFCLRNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGIL 217
            +M+DK LL+E+ LLESK Y  L NLP A  AL +AR TA +IY P   Q A+D+QSGI+
Sbjct: 158 KKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQAALDMQSGII 217

Query: 218 H-AEEKDYCAAFHSFAVALTAFDLFEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGEC 277
           H AEEKD+  A+  F  A    D  + P+A+  LKY+LLC IM +    VQ  +S     
Sbjct: 218 HAAEEKDWKTAYSYFYEAFEGNDSIDSPKAITALKYMLLCKIMLNTPEDVQALVSGKLAL 277

Query: 278 YDKEIEINAMKAVAYATHSKRCSIKLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNL 337
                +  A+K VA A  SK  S+  F+ AL D++A+L++D ++   L+ LY+ L E+NL
Sbjct: 278 RYAGRQTEALKCVAQA--SKNRSLANFEKALTDYKAELRDDPIISTHLAKLYDNLLEQNL 337

Query: 338 YRLIEPFSRVEIVHVANLIELPVDLVESKLSQMILDKKLTGNLDQ--GCLIIFDDPKIDG 397
            R+IEPFSRV+I H+++LI+LP   VE KLSQMILDKK  G LDQ  G LIIFD+P +D 
Sbjct: 338 IRVIEPFSRVQIDHISSLIKLPKPEVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDK 397

Query: 398 IYAATLETIINIGKVVDSLYVRSTK 420
            Y A LETI N+ KVVDSLY ++ K
Sbjct: 398 TYEAALETIQNMSKVVDSLYNKAKK 420

BLAST of MC02g1219 vs. ExPASy Swiss-Prot
Match: Q7KLV9 (26S proteasome non-ATPase regulatory subunit 11 OS=Drosophila melanogaster OX=7227 GN=Rpn6 PE=1 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 3.6e-81
Identity = 174/406 (42.86%), Postives = 260/406 (64.04%), Query Frame = 0

Query: 17  SENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDTDRAEDLRNLVTQLTPL 76
           +  +  S  ++ L R      +  E + IKEQ I+    ++    +A++L +L+    P 
Sbjct: 17  NREEQDSSLLNKLVRDQEGAENDEERIRIKEQGILQQGELYKQEGKAKELADLIKVTRPF 76

Query: 77  FSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAENRTSLRYWVEARLAAL 136
            S ISKAK A++VR ++D+  D+   + +++ LCK+ ++W + E RT LR  +EARL AL
Sbjct: 77  LSSISKAKAAKLVRSLVDMFLDMDAGTGIEVQLCKDCIEWAKQEKRTFLRQSLEARLIAL 136

Query: 137 LIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCLRNLPMANVALYAARIT 196
             +   Y++AL L A+L++E+ +++DK LL+E+ LLESK Y  L NLP A  AL +AR T
Sbjct: 137 YFDTALYTEALALGAQLLRELKKLDDKNLLVEVQLLESKTYHALSNLPKARAALTSARTT 196

Query: 197 AQSIYVPIHQQGAIDLQSGILH-AEEKDYCAAFHSFAVALTAFDLFEDPRAVICLKYLLL 256
           A +IY P   QGA+DLQSGILH A+E+D+  AF  F  A   FD  +  +A+  LKY+LL
Sbjct: 197 ANAIYCPPKVQGALDLQSGILHAADERDFKTAFSYFYEAFEGFDSVDSVKALTSLKYMLL 256

Query: 257 CVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSIKLFKDALRDFRAQLQ 316
           C IM  QS  V   +S          +I+AMK+VA A+H +  S+  F+ AL++++ +L 
Sbjct: 257 CKIMLGQSDDVNQLVSGKLAITYSGRDIDAMKSVAEASHKR--SLADFQAALKEYKKELA 316

Query: 317 EDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVDLVESKLSQMILDKKL 376
           ED++V   L +LY+T+ E+NL R+IEP+SRV++ HVA  I+LP+  VE KLSQMILDKK 
Sbjct: 317 EDVIVQAHLGTLYDTMLEQNLCRIIEPYSRVQVAHVAESIQLPMPQVEKKLSQMILDKKF 376

Query: 377 TGNLDQ--GCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 420
           +G LDQ  G LI+F++  +D  Y   LETI ++GKVVD+LY ++ K
Sbjct: 377 SGILDQGEGVLIVFEETPVDKTYERVLETIQSMGKVVDTLYQKAKK 420

BLAST of MC02g1219 vs. ExPASy Swiss-Prot
Match: Q8BG32 (26S proteasome non-ATPase regulatory subunit 11 OS=Mus musculus OX=10090 GN=Psmd11 PE=1 SV=3)

HSP 1 Score: 301.6 bits (771), Expect = 1.4e-80
Identity = 176/400 (44.00%), Postives = 257/400 (64.25%), Query Frame = 0

Query: 25  AISILYRVLAN--PSSSPEALNIKEQAIIDICYIFNDTDRAEDLRNLVTQLTPLFSLISK 84
           +I IL+ ++      +  EA+ +KEQ+I+++  +   T +A +L  L+  + P  + ISK
Sbjct: 23  SIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLLKYVRPFLNSISK 82

Query: 85  AKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAENRTSLRYWVEARLAALLIEEKD 144
           AK AR+VR ++D+  D+   +  ++ LC E ++W ++E RT LR  +EARL +L  + K 
Sbjct: 83  AKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKR 142

Query: 145 YSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCLRNLPMANVALYAARITAQSIYV 204
           Y +AL+L ++L++E+ +M+DK LL+E+ LLESK Y  L NLP A  AL +AR TA +IY 
Sbjct: 143 YQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYC 202

Query: 205 PIHQQGAIDLQSGILH-AEEKDYCAAFHSFAVALTAFDLFEDPRAVICLKYLLLCVIMCD 264
           P   Q  +D+QSGI+H AEEKD+  A+  F  A   +D  + P+A+  LKY+LLC IM +
Sbjct: 203 PPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLN 262

Query: 265 QSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSIKLFKDALRDFRAQLQEDLLVH 324
               VQ  +S          +  A+K VA A  SK  S+  F+ AL D+RA+L++D ++ 
Sbjct: 263 TPEDVQALVSGKLALRYAGRQTEALKCVAQA--SKNRSLADFEKALTDYRAELRDDPIIS 322

Query: 325 LQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVDLVESKLSQMILDKKLTGNLDQ 384
             L+ LY+ L E+NL R+IEPFSRV+I H+++LI+L    VE KLSQMILDKK  G LDQ
Sbjct: 323 THLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQ 382

Query: 385 --GCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 420
             G LIIFD+P +D  Y A LETI N+ KVVDSLY ++ K
Sbjct: 383 GEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYSKAKK 420

BLAST of MC02g1219 vs. ExPASy Swiss-Prot
Match: Q2KI42 (26S proteasome non-ATPase regulatory subunit 11 OS=Bos taurus OX=9913 GN=PSMD11 PE=2 SV=3)

HSP 1 Score: 301.2 bits (770), Expect = 1.8e-80
Identity = 176/400 (44.00%), Postives = 257/400 (64.25%), Query Frame = 0

Query: 25  AISILYRVLAN--PSSSPEALNIKEQAIIDICYIFNDTDRAEDLRNLVTQLTPLFSLISK 84
           +I IL+ ++      +  EA+ +KEQ+I+++  +   T +A +L  L+  + P  + ISK
Sbjct: 23  SIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLLKYVRPFLNSISK 82

Query: 85  AKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAENRTSLRYWVEARLAALLIEEKD 144
           AK AR+VR ++D+  D+   +  ++ LC E ++W ++E RT LR  +EARL +L  + K 
Sbjct: 83  AKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKR 142

Query: 145 YSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCLRNLPMANVALYAARITAQSIYV 204
           Y +AL+L ++L++E+ +M+DK LL+E+ LLESK Y  L NLP A  AL +AR TA +IY 
Sbjct: 143 YQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYC 202

Query: 205 PIHQQGAIDLQSGILH-AEEKDYCAAFHSFAVALTAFDLFEDPRAVICLKYLLLCVIMCD 264
           P   Q  +D+QSGI+H AEEKD+  A+  F  A   +D  + P+A+  LKY+LLC IM +
Sbjct: 203 PPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLN 262

Query: 265 QSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSIKLFKDALRDFRAQLQEDLLVH 324
               VQ  +S          +  A+K VA A  SK  S+  F+ AL D+RA+L++D ++ 
Sbjct: 263 TPEDVQALVSGKLALRYAGRQTEALKCVAQA--SKNRSLADFEKALTDYRAELRDDPIIS 322

Query: 325 LQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVDLVESKLSQMILDKKLTGNLDQ 384
             L+ LY+ L E+NL R+IEPFSRV+I H+++LI+L    VE KLSQMILDKK  G LDQ
Sbjct: 323 THLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQ 382

Query: 385 --GCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 420
             G LIIFD+P +D  Y A LETI N+ KVVDSLY ++ K
Sbjct: 383 GEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKK 420

BLAST of MC02g1219 vs. NCBI nr
Match: XP_022159089.1 (26S proteasome non-ATPase regulatory subunit 11 homolog [Momordica charantia])

HSP 1 Score: 795 bits (2053), Expect = 2.08e-289
Identity = 419/419 (100.00%), Postives = 419/419 (100.00%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT
Sbjct: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE
Sbjct: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
           NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL
Sbjct: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL
Sbjct: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
           FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI
Sbjct: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD
Sbjct: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360

Query: 361 LVESKLSQMILDKKLTGNLDQGCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 419
           LVESKLSQMILDKKLTGNLDQGCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK
Sbjct: 361 LVESKLSQMILDKKLTGNLDQGCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 419

BLAST of MC02g1219 vs. NCBI nr
Match: XP_023538003.1 (26S proteasome non-ATPase regulatory subunit 11 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496 bits (1277), Expect = 2.37e-171
Identity = 285/421 (67.70%), Postives = 326/421 (77.43%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MS SYLPAT D++  A E  +PSE+ISILYR+L NPSSSPEAL IKEQAI ++  +    
Sbjct: 1   MSTSYLPATTDSIAEALEAKNPSESISILYRILENPSSSPEALRIKEQAITNLSDLLRQE 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           +RAEDLRNL+TQL P F+LI KAKTA+IVR IID +  I  TS+LQISLCKE+VQWTRAE
Sbjct: 61  NRAEDLRNLLTQLRPFFNLIPKAKTAKIVRGIIDAVAKIPDTSDLQISLCKEMVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
            RT LR  VEARLAALL+E K+YS+ALNLLA LIKEV R++DKLLL++I+LLESK++F L
Sbjct: 121 KRTFLRQRVEARLAALLMENKEYSEALNLLAGLIKEVRRLDDKLLLVDIDLLESKLHFSL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLP A  AL AAR  A +IYVP  QQG IDLQSGILHAEEKDY  A+  F  A  AF+ 
Sbjct: 181 RNLPKAKAALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEAFNA 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
            EDPRAV  LKY+LLC IM  Q+  V   IS         +E++AMKAVA A HSKR S+
Sbjct: 241 LEDPRAVFSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGVELDAMKAVADA-HSKR-SL 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLF+ ALRDF+AQL+ED +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LIELPVD
Sbjct: 301 KLFETALRDFKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIELPVD 360

Query: 361 LVESKLSQMILDKKLTGNLDQG--CLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRST 419
            VE KLSQMILDKK  G LDQG  CLIIFDDPK D IY ATLETI NIGKVVDSLYVRS 
Sbjct: 361 HVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIYPATLETISNIGKVVDSLYVRSA 419

BLAST of MC02g1219 vs. NCBI nr
Match: XP_022951123.1 (26S proteasome non-ATPase regulatory subunit 11 homolog [Cucurbita moschata])

HSP 1 Score: 495 bits (1274), Expect = 6.75e-171
Identity = 284/421 (67.46%), Postives = 326/421 (77.43%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MS SYLPAT D++  A E  +PSE+ISILYR+L NPSSSPEAL IKEQAI ++  +    
Sbjct: 1   MSTSYLPATTDSIAEALEAKNPSESISILYRILENPSSSPEALRIKEQAITNLSDLLRQE 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           +RAEDLRNL+TQL P F+LI KAKTA+IVR IID +  I  TS+LQISLCKE+VQWTRAE
Sbjct: 61  NRAEDLRNLLTQLRPFFNLIPKAKTAKIVRGIIDAVAKIPDTSDLQISLCKEMVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
            RT LR  VEARLAALL+E K+YS+ALNLLA LIKEV R++DKLLL++I+LLESK++F L
Sbjct: 121 KRTFLRQRVEARLAALLMENKEYSEALNLLAGLIKEVRRLDDKLLLVDIDLLESKLHFSL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLP A  AL AAR  A +IYVP  QQG IDLQSGILHAEEKDY  A+  F  A  AF+ 
Sbjct: 181 RNLPKAKAALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEAFNA 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
            EDPRAV  LKY+LLC IM  Q+  V   IS         +E++AMKAVA A HSKR S+
Sbjct: 241 LEDPRAVFSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGVELDAMKAVADA-HSKR-SL 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLF+ ALRD++AQL+ED +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LIELPVD
Sbjct: 301 KLFETALRDYKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIELPVD 360

Query: 361 LVESKLSQMILDKKLTGNLDQG--CLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRST 419
            VE KLSQMILDKK  G LDQG  CLIIFDDPK D IY ATLETI NIGKVVDSLYVRS 
Sbjct: 361 HVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIYPATLETISNIGKVVDSLYVRSA 419

BLAST of MC02g1219 vs. NCBI nr
Match: XP_022973290.1 (26S proteasome non-ATPase regulatory subunit 11 homolog [Cucurbita maxima])

HSP 1 Score: 494 bits (1272), Expect = 1.36e-170
Identity = 283/421 (67.22%), Postives = 326/421 (77.43%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MS SYLPAT D++ +A E  +PSE+ISILYR+L NPSSSPEAL IKEQAI ++  +    
Sbjct: 1   MSTSYLPATTDSIAVALEAKNPSESISILYRILENPSSSPEALRIKEQAITNLSDLLRQE 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           +RAEDLRNL+TQL P F+LI KAKTA+IVR IID +  I  TS+LQISLCKE+VQWTRAE
Sbjct: 61  NRAEDLRNLLTQLRPFFNLIPKAKTAKIVRGIIDAVAKIPDTSDLQISLCKEMVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
            RT LR  VEARLAALL+E K+YS+ALNLLA LIKEV R++DKLLL++I+LLESK++F L
Sbjct: 121 KRTFLRQRVEARLAALLMENKEYSEALNLLAGLIKEVRRLDDKLLLVDIDLLESKLHFSL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLP A  AL AAR  A +IYVP  QQG IDLQSGILHAEEKDY  A+  F  A  AF+ 
Sbjct: 181 RNLPKAKAALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEAFNA 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
            EDPRAV  LKY+LLC IM  Q+  V   IS         +E++AMKAVA A HSKR S+
Sbjct: 241 LEDPRAVFSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGVELDAMKAVADA-HSKR-SL 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLF+ ALRD++AQL+ED +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LIELPVD
Sbjct: 301 KLFETALRDYKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIELPVD 360

Query: 361 LVESKLSQMILDKKLTGNLDQG--CLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRST 419
            VE KLSQMILDKK  G LDQG  CLIIFDDPK D IY  TLETI NIGKVVDSLYVRS 
Sbjct: 361 HVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIYPDTLETISNIGKVVDSLYVRSA 419

BLAST of MC02g1219 vs. NCBI nr
Match: KAG6585857.1 (26S proteasome non-ATPase regulatory subunit 11-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 495 bits (1274), Expect = 1.18e-169
Identity = 284/421 (67.46%), Postives = 326/421 (77.43%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MS SYLPAT D++  A E  +PSE+ISILYR+L NPSSSPEAL IKEQAI ++  +    
Sbjct: 84  MSTSYLPATTDSIAEALEAKNPSESISILYRILENPSSSPEALRIKEQAITNLSDLLRQE 143

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           +RAEDLRNL+TQL P F+LI KAKTA+IVR IID +  I  TS+LQISLCKE+VQWTRAE
Sbjct: 144 NRAEDLRNLLTQLRPFFNLIPKAKTAKIVRGIIDAVAKIPDTSDLQISLCKEMVQWTRAE 203

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
            RT LR  VEARLAALL+E K+YS+ALNLLA LIKEV R++DKLLL++I+LLESK++F L
Sbjct: 204 KRTFLRQRVEARLAALLMENKEYSEALNLLAGLIKEVRRLDDKLLLVDIDLLESKLHFSL 263

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLP A  AL AAR  A +IYVP  QQG IDLQSGILHAEEKDY  A+  F  A  AF+ 
Sbjct: 264 RNLPKAKAALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEAFNA 323

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
            EDPRAV  LKY+LLC IM  Q+  V   IS         +E++AMKAVA A HSKR S+
Sbjct: 324 LEDPRAVFSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGVELDAMKAVADA-HSKR-SL 383

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLF+ ALRD++AQL+ED +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LIELPVD
Sbjct: 384 KLFETALRDYKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIELPVD 443

Query: 361 LVESKLSQMILDKKLTGNLDQG--CLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRST 419
            VE KLSQMILDKK  G LDQG  CLIIFDDPK D IY ATLETI NIGKVVDSLYVRS 
Sbjct: 444 HVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIYPATLETISNIGKVVDSLYVRSA 502

BLAST of MC02g1219 vs. ExPASy TrEMBL
Match: A0A6J1E1C5 (26S proteasome non-ATPase regulatory subunit 11 homolog OS=Momordica charantia OX=3673 GN=LOC111025532 PE=3 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 1.01e-289
Identity = 419/419 (100.00%), Postives = 419/419 (100.00%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT
Sbjct: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE
Sbjct: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
           NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL
Sbjct: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL
Sbjct: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
           FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI
Sbjct: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD
Sbjct: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360

Query: 361 LVESKLSQMILDKKLTGNLDQGCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 419
           LVESKLSQMILDKKLTGNLDQGCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK
Sbjct: 361 LVESKLSQMILDKKLTGNLDQGCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 419

BLAST of MC02g1219 vs. ExPASy TrEMBL
Match: A0A6J1GGU2 (26S proteasome non-ATPase regulatory subunit 11 homolog OS=Cucurbita moschata OX=3662 GN=LOC111454065 PE=3 SV=1)

HSP 1 Score: 495 bits (1274), Expect = 3.27e-171
Identity = 284/421 (67.46%), Postives = 326/421 (77.43%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MS SYLPAT D++  A E  +PSE+ISILYR+L NPSSSPEAL IKEQAI ++  +    
Sbjct: 1   MSTSYLPATTDSIAEALEAKNPSESISILYRILENPSSSPEALRIKEQAITNLSDLLRQE 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           +RAEDLRNL+TQL P F+LI KAKTA+IVR IID +  I  TS+LQISLCKE+VQWTRAE
Sbjct: 61  NRAEDLRNLLTQLRPFFNLIPKAKTAKIVRGIIDAVAKIPDTSDLQISLCKEMVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
            RT LR  VEARLAALL+E K+YS+ALNLLA LIKEV R++DKLLL++I+LLESK++F L
Sbjct: 121 KRTFLRQRVEARLAALLMENKEYSEALNLLAGLIKEVRRLDDKLLLVDIDLLESKLHFSL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLP A  AL AAR  A +IYVP  QQG IDLQSGILHAEEKDY  A+  F  A  AF+ 
Sbjct: 181 RNLPKAKAALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEAFNA 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
            EDPRAV  LKY+LLC IM  Q+  V   IS         +E++AMKAVA A HSKR S+
Sbjct: 241 LEDPRAVFSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGVELDAMKAVADA-HSKR-SL 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLF+ ALRD++AQL+ED +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LIELPVD
Sbjct: 301 KLFETALRDYKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIELPVD 360

Query: 361 LVESKLSQMILDKKLTGNLDQG--CLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRST 419
            VE KLSQMILDKK  G LDQG  CLIIFDDPK D IY ATLETI NIGKVVDSLYVRS 
Sbjct: 361 HVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIYPATLETISNIGKVVDSLYVRSA 419

BLAST of MC02g1219 vs. ExPASy TrEMBL
Match: A0A6J1IAZ8 (26S proteasome non-ATPase regulatory subunit 11 homolog OS=Cucurbita maxima OX=3661 GN=LOC111471857 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 6.58e-171
Identity = 283/421 (67.22%), Postives = 326/421 (77.43%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MS SYLPAT D++ +A E  +PSE+ISILYR+L NPSSSPEAL IKEQAI ++  +    
Sbjct: 1   MSTSYLPATTDSIAVALEAKNPSESISILYRILENPSSSPEALRIKEQAITNLSDLLRQE 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           +RAEDLRNL+TQL P F+LI KAKTA+IVR IID +  I  TS+LQISLCKE+VQWTRAE
Sbjct: 61  NRAEDLRNLLTQLRPFFNLIPKAKTAKIVRGIIDAVAKIPDTSDLQISLCKEMVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
            RT LR  VEARLAALL+E K+YS+ALNLLA LIKEV R++DKLLL++I+LLESK++F L
Sbjct: 121 KRTFLRQRVEARLAALLMENKEYSEALNLLAGLIKEVRRLDDKLLLVDIDLLESKLHFSL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLP A  AL AAR  A +IYVP  QQG IDLQSGILHAEEKDY  A+  F  A  AF+ 
Sbjct: 181 RNLPKAKAALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEAFNA 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
            EDPRAV  LKY+LLC IM  Q+  V   IS         +E++AMKAVA A HSKR S+
Sbjct: 241 LEDPRAVFSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGVELDAMKAVADA-HSKR-SL 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLF+ ALRD++AQL+ED +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LIELPVD
Sbjct: 301 KLFETALRDYKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIELPVD 360

Query: 361 LVESKLSQMILDKKLTGNLDQG--CLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRST 419
            VE KLSQMILDKK  G LDQG  CLIIFDDPK D IY  TLETI NIGKVVDSLYVRS 
Sbjct: 361 HVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIYPDTLETISNIGKVVDSLYVRSA 419

BLAST of MC02g1219 vs. ExPASy TrEMBL
Match: M5XE62 (PCI domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_1G279500 PE=3 SV=1)

HSP 1 Score: 490 bits (1262), Expect = 2.17e-169
Identity = 281/421 (66.75%), Postives = 325/421 (77.20%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MS SYLPAT D++  A E  +PSEAISILYR+L NPSSS EAL IKEQAI ++  +    
Sbjct: 1   MSSSYLPATTDSIAQALEAKAPSEAISILYRILENPSSSSEALRIKEQAIANLTDLLRQE 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           +RAEDLRNL+TQL P F+LI KAKTA+IVR IID +  + GTS+LQISLCKE+VQWTRAE
Sbjct: 61  NRAEDLRNLLTQLRPFFNLIPKAKTAKIVRSIIDAVAKVPGTSDLQISLCKEMVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
            RT LR  VEARLA+LL+E K+YS+AL+LL+ LIKEV R++DKLLL++I+LLESK++F L
Sbjct: 121 KRTFLRQRVEARLASLLMESKEYSEALSLLSGLIKEVRRLDDKLLLVDIDLLESKLHFSL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLP A  +L AAR  A SIYVP  QQG IDLQSGILHAEEKDY  A+  F  A  AF+ 
Sbjct: 181 RNLPKAKASLTAARTAANSIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEAFNA 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
            EDPRAV  LKY+LLC IM  Q+  V   IS          E++AMKAVA A HSKR S+
Sbjct: 241 LEDPRAVFSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGPELDAMKAVADA-HSKR-SL 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLF+ ALRDF+AQL+ED +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LIELP+D
Sbjct: 301 KLFETALRDFKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIELPID 360

Query: 361 LVESKLSQMILDKKLTGNLDQG--CLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRST 419
            VE KLSQMILDKK  G LDQG  CLIIFDDPK D IY ATLETI NIGKVVDSLYVRS 
Sbjct: 361 HVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIYPATLETISNIGKVVDSLYVRSA 419

BLAST of MC02g1219 vs. ExPASy TrEMBL
Match: A0A6J5WAJ2 (PCI domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS6603 PE=3 SV=1)

HSP 1 Score: 490 bits (1262), Expect = 2.17e-169
Identity = 281/421 (66.75%), Postives = 325/421 (77.20%), Query Frame = 0

Query: 1   MSMSYLPATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDT 60
           MS SYLPAT D++  A E  +PSEAISILYR+L NPSSS EAL IKEQAI ++  +    
Sbjct: 1   MSSSYLPATTDSIAQALEAKAPSEAISILYRILENPSSSSEALRIKEQAITNLTDLLRQE 60

Query: 61  DRAEDLRNLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAE 120
           +RAEDLRNL+TQL P F+LI KAKTA+IVR IID +  + GTS+LQISLCKE+VQWTRAE
Sbjct: 61  NRAEDLRNLLTQLRPFFNLIPKAKTAKIVRSIIDAVAKVPGTSDLQISLCKEMVQWTRAE 120

Query: 121 NRTSLRYWVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCL 180
            RT LR  VEARLA+LL+E K+YS+AL+LL+ LIKEV R++DKLLL++I+LLESK++F L
Sbjct: 121 KRTFLRQRVEARLASLLMESKEYSEALSLLSGLIKEVRRLDDKLLLVDIDLLESKLHFSL 180

Query: 181 RNLPMANVALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDL 240
           RNLP A  +L AAR  A SIYVP  QQG IDLQSGILHAEEKDY  A+  F  A  AF+ 
Sbjct: 181 RNLPKAKASLTAARTAANSIYVPPAQQGTIDLQSGILHAEEKDYKTAYSYFFEAFEAFNA 240

Query: 241 FEDPRAVICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSI 300
            EDPRAV  LKY+LLC IM  Q+  V   IS          E++AMKAVA A HSKR S+
Sbjct: 241 LEDPRAVFSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGPELDAMKAVADA-HSKR-SL 300

Query: 301 KLFKDALRDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVD 360
           KLF+ ALRDF+AQL+ED +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LIELP+D
Sbjct: 301 KLFETALRDFKAQLEEDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIELPID 360

Query: 361 LVESKLSQMILDKKLTGNLDQG--CLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRST 419
            VE KLSQMILDKK  G LDQG  CLIIFDDPK D IY ATLETI NIGKVVDSLYVRS 
Sbjct: 361 HVEKKLSQMILDKKFAGTLDQGAGCLIIFDDPKTDAIYPATLETISNIGKVVDSLYVRSA 419

BLAST of MC02g1219 vs. TAIR 10
Match: AT1G29150.1 (non-ATPase subunit 9 )

HSP 1 Score: 458.4 bits (1178), Expect = 6.3e-129
Identity = 261/414 (63.04%), Postives = 316/414 (76.33%), Query Frame = 0

Query: 8   ATMDTLILASENDSPSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDTDRAEDLR 67
           AT +T+ LA E +S SEAI+ILY+VL +PSSSPEA+ IKEQAI ++C    +  R EDLR
Sbjct: 6   ATTETISLALEANS-SEAITILYQVLEDPSSSPEAIRIKEQAITNLCDRLTEEKRGEDLR 65

Query: 68  NLVTQLTPLFSLISKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWTRAENRTSLRY 127
            L+T+L P FSLI KAKTA+IVR IID +  I GT++LQI+LCKE+V+WTRAE RT LR 
Sbjct: 66  KLLTKLRPFFSLIPKAKTAKIVRGIIDAVAKIPGTTDLQITLCKEMVEWTRAEKRTFLRQ 125

Query: 128 WVEARLAALLIEEKDYSDALNLLAELIKEVGRMEDKLLLIEINLLESKIYFCLRNLPMAN 187
            VEARLAALL+E K+Y +AL LL+ L+KEV R++DKLLL++I+LLESK++F LRNLP A 
Sbjct: 126 RVEARLAALLMENKEYVEALALLSTLVKEVRRLDDKLLLVDIDLLESKLHFSLRNLPKAK 185

Query: 188 VALYAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDLFEDPRAV 247
            AL AAR  A +IYVP  QQG IDLQSGILHAEEKDY   +  F  A  +F+   DPRAV
Sbjct: 186 AALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTGYSYFFEAFESFNALGDPRAV 245

Query: 248 ICLKYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSIKLFKDAL 307
             LKY+LLC IM  Q+  V   IS          +++AMKAVA A HSKR S+KLF++AL
Sbjct: 246 FSLKYMLLCKIMVSQADDVAGIISSKAGLQYVGPDLDAMKAVADA-HSKR-SLKLFENAL 305

Query: 308 RDFRAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVDLVESKLS 367
           RD++AQL++D +VH  LSSLY+TL E+NL RLIEPFSRVEI H+A LI LP+D VE KLS
Sbjct: 306 RDYKAQLEDDPIVHRHLSSLYDTLLEQNLCRLIEPFSRVEIAHIAELIGLPLDHVEKKLS 365

Query: 368 QMILDKKLTGNLDQ--GCLIIFDDPKIDGIYAATLETIINIGKVVDSLYVRSTK 420
           QMILDKK  G LDQ  GCLIIF+DPK D IY+ATLETI N+GKVVDSLYVRS K
Sbjct: 366 QMILDKKFAGTLDQGAGCLIIFEDPKADAIYSATLETIANMGKVVDSLYVRSAK 416

BLAST of MC02g1219 vs. TAIR 10
Match: AT2G26990.1 (proteasome family protein )

HSP 1 Score: 60.1 bits (144), Expect = 5.0e-09
Identity = 83/371 (22.37%), Postives = 163/371 (43.94%), Query Frame = 0

Query: 22  PSEAISILYRVLANPSSSPEALNIKEQAIIDICYIFNDTDRAEDLRNLVTQ-LTPLFSLI 81
           P EA+S    V+      PE  +   +A+     I+    + +++    T+ LT + S +
Sbjct: 41  PEEALSGFAEVV---QMEPEKADWGFKALKQTVKIYYRLGKYKEMMEAYTEMLTYIKSAV 100

Query: 82  SKAKTARIVRDIIDVIGDITGTSELQISLCKELVQWT--RAENRTSLRYWVEA--RLAAL 141
           ++  + + + +I+D    ++G++     L +E  Q T    E   + R W +   +L  +
Sbjct: 101 TRNYSEKCINNIMDF---VSGSASQNTGLLQEFYQTTLKALEEAKNERLWFKTNLKLCNI 160

Query: 142 LIEEKDYSDALNLLAEL----IKEVGRMEDK--LLLIEINLLESKIYFCLRNLPMANVAL 201
             +  +Y     +L EL     KE G  + K    L+E+  +E +IY   ++       L
Sbjct: 161 WFDIGEYRRMTKILKELHKSCQKEDGTDDQKKGSQLLEVYAIEIQIYTETKDNKKLK-QL 220

Query: 202 YAARITAQSIYVPIHQQGAIDLQSGILHAEEKDYCAAFHSFAVALTAFDLFEDPRAVICL 261
           Y   +  +S        G I    G +H  E+ +  A   F  A   +D   + R + CL
Sbjct: 221 YHKALAIKSAIPHPRIMGIIRECGGKMHMAERQWEEAATDFFEAFKNYDEAGNQRRIQCL 280

Query: 262 KYLLLCVIMCDQSYLVQFAISRYGECYDKEIEINAMKAVAYATHSKRCSIKLFKDALRDF 321
           KYL+L  ++ +    V     +  + Y  + EI AM  +  A   +R  I  F+  L+  
Sbjct: 281 KYLVLANMLMESE--VNPFDGQEAKPYKNDPEILAMTNLIAA--YQRNEIIEFERILKSN 340

Query: 322 RAQLQEDLLVHLQLSSLYNTLFEKNLYRLIEPFSRVEIVHVANLIELPVDLVESKLSQMI 381
           R  + +D  +   +  L   +  + L +LI+P++++ I  ++  + +P   V   L  +I
Sbjct: 341 RRTIMDDPFIRNYMEDLLKKVRTQVLLKLIKPYTKIGIPFISKELNVPETDVTELLVSLI 400

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LP458.9e-12863.0426S proteasome non-ATPase regulatory subunit 11 homolog OS=Arabidopsis thaliana ... [more]
F6XBL27.3e-8245.9726S proteasome non-ATPase regulatory subunit 11 OS=Xenopus tropicalis OX=8364 GN... [more]
Q7KLV93.6e-8142.8626S proteasome non-ATPase regulatory subunit 11 OS=Drosophila melanogaster OX=72... [more]
Q8BG321.4e-8044.0026S proteasome non-ATPase regulatory subunit 11 OS=Mus musculus OX=10090 GN=Psmd... [more]
Q2KI421.8e-8044.0026S proteasome non-ATPase regulatory subunit 11 OS=Bos taurus OX=9913 GN=PSMD11 ... [more]
Match NameE-valueIdentityDescription
XP_022159089.12.08e-289100.0026S proteasome non-ATPase regulatory subunit 11 homolog [Momordica charantia][more]
XP_023538003.12.37e-17167.7026S proteasome non-ATPase regulatory subunit 11 homolog [Cucurbita pepo subsp. p... [more]
XP_022951123.16.75e-17167.4626S proteasome non-ATPase regulatory subunit 11 homolog [Cucurbita moschata][more]
XP_022973290.11.36e-17067.2226S proteasome non-ATPase regulatory subunit 11 homolog [Cucurbita maxima][more]
KAG6585857.11.18e-16967.4626S proteasome non-ATPase regulatory subunit 11-like protein, partial [Cucurbita... [more]
Match NameE-valueIdentityDescription
A0A6J1E1C51.01e-289100.0026S proteasome non-ATPase regulatory subunit 11 homolog OS=Momordica charantia O... [more]
A0A6J1GGU23.27e-17167.4626S proteasome non-ATPase regulatory subunit 11 homolog OS=Cucurbita moschata OX... [more]
A0A6J1IAZ86.58e-17167.2226S proteasome non-ATPase regulatory subunit 11 homolog OS=Cucurbita maxima OX=3... [more]
M5XE622.17e-16966.75PCI domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_1G279500 PE=3 S... [more]
A0A6J5WAJ22.17e-16966.75PCI domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS6603 P... [more]
Match NameE-valueIdentityDescription
AT1G29150.16.3e-12963.04non-ATPase subunit 9 [more]
AT2G26990.15.0e-0922.37proteasome family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 143..163
NoneNo IPR availableSMARTSM00753PINTcoord: 322..403
e-value: 2.0E-12
score: 57.3
NoneNo IPR availableGENE3D1.25.40.570coord: 46..419
e-value: 2.0E-115
score: 387.9
NoneNo IPR availablePANTHERPTHR10678:SF1126S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11-LIKE PROTEINcoord: 3..419
NoneNo IPR availablePANTHERPTHR1067826S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2coord: 3..419
IPR000717Proteasome component (PCI) domainSMARTSM00088PINT_4coord: 322..403
e-value: 2.0E-12
score: 57.3
IPR000717Proteasome component (PCI) domainPFAMPF01399PCIcoord: 293..381
e-value: 9.1E-14
score: 51.9
IPR0407806S proteasome subunit Rpn6, C-terminal helix domainPFAMPF18503RPN6_C_helixcoord: 393..417
e-value: 1.4E-8
score: 34.3
IPR04077326S proteasome regulatory subunit Rpn6, N-terminalPFAMPF18055RPN6_Ncoord: 19..126
e-value: 4.1E-26
score: 91.6
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 317..393

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g1219.1MC02g1219.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0008541 proteasome regulatory particle, lid subcomplex
molecular_function GO:0005198 structural molecule activity