MC02g1001 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g1001
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Alpha/beta-Hydrolases superfamily protein, putative
Location	MC02: 8025311 .. 8026186 (+)
RNA-Seq Expression	MC02g1001
Synteny	MC02g1001

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGTAGTTTTCGAATTCCAACCGGTCTTCCGCGTCTTCAGAAGCGGCCGCGTCGAGAGGCTCATTCCCGGCGACCGCGTTCCGCCGTCGACTGATCAGCGGACCGGCGTGCAGTCCAAAGACATCCTCATCGATCCACAAACTGGAGTCTCCGTCCGCATTTACCTCCCGCCGGTTCCGGATTCAACAAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCATTCGCAATCGGCTCCGCCGATTCCGCCATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAAGCGAACGCCGTCGCTCTGTCGGTGAACTACCGGCTGGCGCCTGAGCATCCTCTCCCTGCGGCGTACGACGACGCCTGGACGTCGGTGGAATGGGCCGCCTCGCACGCGAATCGCGACGGCGCGGAGATTTGGCTGAACAACCACGCGGATTTTGATCGCGTTTTCCTTGTTGGAGACAGCGCTGGGGCCAACATTTCGTACAACTTAGTGGGCCGGGCTGGTAATGGGCCGGCGGGATTGAACATTGTTGGGCTGGGCCTGATCCATCCGTTTTTCATGATAGACAAGCCCGATAAGCTGATCGAGTACATCTTCCCGACGTCGGCGGGTCTGGAGGATCCGAGGATGAACCCGAGAGCGAACCCGGAACTACGGAGCCTACGGTGCACCCAGGTGGTGATACTCGTGGCGGAGAATGATTGGCTGAAGGATAGGGGGCGTGGGTTTCACGCGGCGCTGAGGGAGAGTGGGTGGAAGGGGTCGGTGGAGATTGTGGAAACGGAGGGGGAGGGCCACGTGTTCCATTTGTTTAATCCGGAGAACGAGAAGGCTGCTACGTTGGTGAAACAGGTGGCTTCCTTCATCCAA

mRNA sequence

GAAGTAGTTTTCGAATTCCAACCGGTCTTCCGCGTCTTCAGAAGCGGCCGCGTCGAGAGGCTCATTCCCGGCGACCGCGTTCCGCCGTCGACTGATCAGCGGACCGGCGTGCAGTCCAAAGACATCCTCATCGATCCACAAACTGGAGTCTCCGTCCGCATTTACCTCCCGCCGGTTCCGGATTCAACAAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCATTCGCAATCGGCTCCGCCGATTCCGCCATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAAGCGAACGCCGTCGCTCTGTCGGTGAACTACCGGCTGGCGCCTGAGCATCCTCTCCCTGCGGCGTACGACGACGCCTGGACGTCGGTGGAATGGGCCGCCTCGCACGCGAATCGCGACGGCGCGGAGATTTGGCTGAACAACCACGCGGATTTTGATCGCGTTTTCCTTGTTGGAGACAGCGCTGGGGCCAACATTTCGTACAACTTAGTGGGCCGGGCTGGTAATGGGCCGGCGGGATTGAACATTGTTGGGCTGGGCCTGATCCATCCGTTTTTCATGATAGACAAGCCCGATAAGCTGATCGAGTACATCTTCCCGACGTCGGCGGGTCTGGAGGATCCGAGGATGAACCCGAGAGCGAACCCGGAACTACGGAGCCTACGGTGCACCCAGGTGGTGATACTCGTGGCGGAGAATGATTGGCTGAAGGATAGGGGGCGTGGGTTTCACGCGGCGCTGAGGGAGAGTGGGTGGAAGGGGTCGGTGGAGATTGTGGAAACGGAGGGGGAGGGCCACGTGTTCCATTTGTTTAATCCGGAGAACGAGAAGGCTGCTACGTTGGTGAAACAGGTGGCTTCCTTCATCCAA

Coding sequence (CDS)

GAAGTAGTTTTCGAATTCCAACCGGTCTTCCGCGTCTTCAGAAGCGGCCGCGTCGAGAGGCTCATTCCCGGCGACCGCGTTCCGCCGTCGACTGATCAGCGGACCGGCGTGCAGTCCAAAGACATCCTCATCGATCCACAAACTGGAGTCTCCGTCCGCATTTACCTCCCGCCGGTTCCGGATTCAACAAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCATTCGCAATCGGCTCCGCCGATTCCGCCATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAAGCGAACGCCGTCGCTCTGTCGGTGAACTACCGGCTGGCGCCTGAGCATCCTCTCCCTGCGGCGTACGACGACGCCTGGACGTCGGTGGAATGGGCCGCCTCGCACGCGAATCGCGACGGCGCGGAGATTTGGCTGAACAACCACGCGGATTTTGATCGCGTTTTCCTTGTTGGAGACAGCGCTGGGGCCAACATTTCGTACAACTTAGTGGGCCGGGCTGGTAATGGGCCGGCGGGATTGAACATTGTTGGGCTGGGCCTGATCCATCCGTTTTTCATGATAGACAAGCCCGATAAGCTGATCGAGTACATCTTCCCGACGTCGGCGGGTCTGGAGGATCCGAGGATGAACCCGAGAGCGAACCCGGAACTACGGAGCCTACGGTGCACCCAGGTGGTGATACTCGTGGCGGAGAATGATTGGCTGAAGGATAGGGGGCGTGGGTTTCACGCGGCGCTGAGGGAGAGTGGGTGGAAGGGGTCGGTGGAGATTGTGGAAACGGAGGGGGAGGGCCACGTGTTCCATTTGTTTAATCCGGAGAACGAGAAGGCTGCTACGTTGGTGAAACAGGTGGCTTCCTTCATCCAA

Protein sequence

EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ

Homology

[Close]

BLAST of MC02g1001 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-65
Identity = 136/308 (44.16%), Postives = 190/308 (61.69%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP-PV 60
           +V  E  P   V   G VERL   +  PP  D  TGV SKDI+I+P+TG+S RIY P  +
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 61  PDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
               K+PL++Y HGGAF I S     YH  L  +  +AN +A+SVNYRLAPEHPLP AY+
Sbjct: 67  QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYE 126

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIV 180
           D+WT+++   +       E W+N++AD D +FLVGDSAGANIS++L  RA      L I 
Sbjct: 127 DSWTALKNIQAI-----NEPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTLKIK 186

Query: 181 GLGLIHPFFMIDKP--------------DKLIEYIFPTSAGLEDPRMNPRA--NPELRSL 240
           G+G+IHP+F   +P              D   E++ P+  G +DP +NP A  +P+L  L
Sbjct: 187 GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDLGGL 246

Query: 241 RCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATL 292
            C +V+I VAE D L +RG+ ++  L +S WKG VEI+ET+ + HVFH+F P+ ++A  +
Sbjct: 247 GCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEAMEM 306

[Close]

BLAST of MC02g1001 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.3e-64
Identity = 127/312 (40.71%), Postives = 187/312 (59.94%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPP-- 60
           E+ F++ P FR+F++G +ERL+P   VPPS +   GV SKD +  P+  +S+RIYLP   
Sbjct: 4   EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNS 63

Query: 61  --VPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPA 120
                  K+PLL+Y HGG F + +A S IYH  L S  +  + +A+SV YR APEHP+P 
Sbjct: 64  VYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPT 123

Query: 121 AYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN---GP 180
            Y+D+W +++W  +H  R G E WLN HADF +VFL GDSAGANI++++  R       P
Sbjct: 124 LYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPP 183

Query: 181 AGLNIVGLGLIHPFFM------------IDKPDKLIEYIFPTSA-GLEDPRMNPRANPEL 240
               I G+ L HP+F+            +   ++L     P S  G+EDP +N     +L
Sbjct: 184 ENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV-VGSDL 243

Query: 241 RSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKA 293
             L C +V+++VA ND L   G  + A L +SGW G V+++ET+ EGHVFHL +P++E A
Sbjct: 244 TGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENA 303

[Close]

BLAST of MC02g1001 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.7e-64
Identity = 135/323 (41.80%), Postives = 198/323 (61.30%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP--- 60
           E+  ++ P+  +++SGR+ERL+    VPPS++ + GV SKD++  P   +S+RIYLP   
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 61  ---PVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPL 120
                  S KLPLL+Y HGG F + +A S  YH  L +  + ++ VA+SV+YR APEHP+
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 121 PAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN--- 180
           P +YDD+WT+++W  SH    G+E WLN HADF +VFL GDSAGANI++++  +A     
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 181 GPAGLN---IVGLGLIHPFFMIDKP--DK---------LIEYIFPTSA-----GLEDPRM 240
            P  LN   I G+ L+HP+F    P  DK          IE ++  ++     G +DP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 241 N--PRANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSV-EIVETEGEGHV 293
           N     + +L  L C +V+++VAE D L  +G G+   L +S W G V ++VET+GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

[Close]

BLAST of MC02g1001 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 2.8e-64
Identity = 135/318 (42.45%), Postives = 192/318 (60.38%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP--- 60
           E+  +  P+ ++++SGR+ERL+    VPPS++ + GV SKD++      +SVRIYLP   
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 61  PVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAA 120
                +KLPLL+Y HGG F I +A S  YH  L +  + +N VA+SV+YR APEHP+   
Sbjct: 64  AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 123

Query: 121 YDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNG--PAG 180
           +DD+WT+++W  +H    G E WLN HADF RVFL GDSAGANI +++  RA       G
Sbjct: 124 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPG 183

Query: 181 LN---IVGLGLIHPFFM----IDKPDKLIEYI-----------FPTSA-GLEDPRMN--P 240
           LN   I G+ L+HP+F     ID+ D   E +            P S  G +DP +N   
Sbjct: 184 LNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQ 243

Query: 241 RANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFN 293
             + +L  L C +V+++VAE D L  +G G+ A L +SGWKG VE+VE+EGE HVFHL  
Sbjct: 244 SESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLK 303

[Close]

BLAST of MC02g1001 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 6.3e-64
Identity = 128/310 (41.29%), Postives = 189/310 (60.97%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           E+V + +P   +++SGR+ER + G  V P+  +   V +KD++IDP TGVSVR+YLP V 
Sbjct: 82  EIVLDLKPFLIIYKSGRIERFL-GTTVIPACPE---VATKDVVIDPATGVSVRLYLPNVV 141

Query: 61  D--STKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAY 120
           D  S KLP+L+Y HGG F I +  S  YH +L  LAA+A  + +S+NYRLAPE+PLPA+Y
Sbjct: 142 DLPSKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAPEYPLPASY 201

Query: 121 DDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNI 180
           DD      W  SH+     E WL  H DF ++ L GDSAG N+++ +  RA  G     I
Sbjct: 202 DDCMAGFNWVVSHSAGPALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRADAGV----I 261

Query: 181 VGLGLIHPFFMIDKP--------------DKLIEYIFPTSAGLEDPRMNPRA--NPELRS 240
            G+ ++HP+F+  +P              DKL     P + GL+DP +NP A   P L  
Sbjct: 262 EGVAIVHPYFLGSEPVGNEINDPANIEFHDKLWRLAAPDTEGLDDPLINPVAPGAPSLAG 321

Query: 241 LRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAAT 293
           L+C + V+ VA ND+L +RGR ++ AL +SGW+G  E+V+ EG GHVFHL +   + +  
Sbjct: 322 LKCKRAVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGVGHVFHLSDYSGDISVA 381

[Close]

BLAST of MC02g1001 vs. NCBI nr
Match: XP_022148541.1 (probable carboxylesterase 2 [Momordica charantia])

HSP 1 Score: 594 bits (1532), Expect = 5.29e-214
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP
Sbjct: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 67

Query: 61  DSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDD 120
           DSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDD
Sbjct: 68  DSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDD 127

Query: 121 AWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIVG 180
           AWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIVG
Sbjct: 128 AWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIVG 187

Query: 181 LGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLKD 240
           LGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLKD
Sbjct: 188 LGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLKD 247

Query: 241 RGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 292
           RGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ
Sbjct: 248 RGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 299

[Close]

BLAST of MC02g1001 vs. NCBI nr
Match: KAG6605131.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 474 bits (1219), Expect = 2.88e-166
Identity = 234/296 (79.05%), Postives = 256/296 (86.49%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVRIYLP V 
Sbjct: 8   EVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVRIYLPKVS 67

Query: 61  DSTK-LPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
            S K LPLLIYIHGGAFAIGS  S+IYH HLISLAAEANAVA+SV YRLAPE+PLPAAYD
Sbjct: 68  TSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYD 127

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG---NGPAGL 180
           DAW +VEW ASH+NRDGAE+WLN HADFDRVF+VGDSAGANISYN+VGRA    NGP GL
Sbjct: 128 DAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASENDNGPEGL 187

Query: 181 NIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAEND 240
            I+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L  LRC +V+I+ AE+D
Sbjct: 188 KIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRLRCNRVLIIAAEHD 247

Query: 241 WLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 292
           +L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK VASFIQ
Sbjct: 248 FLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKTVASFIQ 303

[Close]

BLAST of MC02g1001 vs. NCBI nr
Match: XP_023007510.1 (probable carboxylesterase 2 [Cucurbita maxima])

HSP 1 Score: 473 bits (1218), Expect = 4.09e-166
Identity = 233/296 (78.72%), Postives = 256/296 (86.49%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVRIYLP V 
Sbjct: 8   EVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVRIYLPKVS 67

Query: 61  DST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
            S  KLPLLIYIHGGAF IGS  S+IYH HLISLAAEANAVA+SV YRLAPE+PLPAAYD
Sbjct: 68  TSAEKLPLLIYIHGGAFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYD 127

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG---NGPAGL 180
           DAWT+VEW ASH+NRDGAE+WLN HADFD VF+VGDSAGANISYN+VGRA    NGP GL
Sbjct: 128 DAWTAVEWVASHSNRDGAEMWLNEHADFDCVFIVGDSAGANISYNVVGRASENDNGPEGL 187

Query: 181 NIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAEND 240
           NI+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L   RC +V+I+ AE+D
Sbjct: 188 NIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLARFRCNRVLIIAAEHD 247

Query: 241 WLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 292
           +L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK+VASFIQ
Sbjct: 248 FLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVASFIQ 303

[Close]

BLAST of MC02g1001 vs. NCBI nr
Match: KAG7035128.1 (putative carboxylesterase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 473 bits (1216), Expect = 8.24e-166
Identity = 233/296 (78.72%), Postives = 256/296 (86.49%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGV+SKD+LIDP T +SVRIYLP V 
Sbjct: 8   EVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVESKDVLIDPHTKLSVRIYLPKVS 67

Query: 61  DSTK-LPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
            S K LPLLIYIHGGAFAIGS  S+IYH HLISLAAEANAVA+SV YRLAPE+PLPAAYD
Sbjct: 68  TSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYD 127

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG---NGPAGL 180
           DAW +VEW ASH+NRDGAE+WLN HADFDRVF+VGDSAGANISYN+VGRA    NGP GL
Sbjct: 128 DAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASENDNGPEGL 187

Query: 181 NIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAEND 240
            I+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L  LRC +V+I+ AE+D
Sbjct: 188 KIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRLRCNRVLIIAAEHD 247

Query: 241 WLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 292
           +L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK VASFIQ
Sbjct: 248 FLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKTVASFIQ 303

[Close]

BLAST of MC02g1001 vs. NCBI nr
Match: XP_023532126.1 (probable carboxylesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 472 bits (1214), Expect = 1.66e-165
Identity = 232/296 (78.38%), Postives = 256/296 (86.49%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVRIYLP V 
Sbjct: 8   EVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVRIYLPKVS 67

Query: 61  DSTK-LPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
            S K LPLLIYIHGGAF+IGS  S+IYH HLISLAAEANAVA+SV YRLAPE+PLPAAYD
Sbjct: 68  TSAKKLPLLIYIHGGAFSIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYD 127

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG---NGPAGL 180
           DAW +VEW ASH+NRDGAE+WLN HADFDRVF+VGDSAGANISYN+VGRA    NGP GL
Sbjct: 128 DAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASENDNGPEGL 187

Query: 181 NIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAEND 240
            I+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L   RC +V+I+ AE+D
Sbjct: 188 KIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNRVLIIAAEHD 247

Query: 241 WLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 292
           +L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK+VASFIQ
Sbjct: 248 FLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVASFIQ 303

[Close]

BLAST of MC02g1001 vs. ExPASy TrEMBL
Match: A0A6J1D5P6 (probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 SV=1)

HSP 1 Score: 594 bits (1532), Expect = 2.56e-214
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP
Sbjct: 8   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 67

Query: 61  DSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDD 120
           DSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDD
Sbjct: 68  DSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDD 127

Query: 121 AWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIVG 180
           AWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIVG
Sbjct: 128 AWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIVG 187

Query: 181 LGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLKD 240
           LGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLKD
Sbjct: 188 LGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLKD 247

Query: 241 RGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 292
           RGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ
Sbjct: 248 RGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 299

[Close]

BLAST of MC02g1001 vs. ExPASy TrEMBL
Match: A0A6J1L361 (probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=1)

HSP 1 Score: 473 bits (1218), Expect = 1.98e-166
Identity = 233/296 (78.72%), Postives = 256/296 (86.49%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVRIYLP V 
Sbjct: 8   EVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVRIYLPKVS 67

Query: 61  DST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
            S  KLPLLIYIHGGAF IGS  S+IYH HLISLAAEANAVA+SV YRLAPE+PLPAAYD
Sbjct: 68  TSAEKLPLLIYIHGGAFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYD 127

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG---NGPAGL 180
           DAWT+VEW ASH+NRDGAE+WLN HADFD VF+VGDSAGANISYN+VGRA    NGP GL
Sbjct: 128 DAWTAVEWVASHSNRDGAEMWLNEHADFDCVFIVGDSAGANISYNVVGRASENDNGPEGL 187

Query: 181 NIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAEND 240
           NI+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L   RC +V+I+ AE+D
Sbjct: 188 NIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLARFRCNRVLIIAAEHD 247

Query: 241 WLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 292
           +L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+ EKA  LVK+VASFIQ
Sbjct: 248 FLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVASFIQ 303

[Close]

BLAST of MC02g1001 vs. ExPASy TrEMBL
Match: A0A6J1G929 (probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 SV=1)

HSP 1 Score: 469 bits (1208), Expect = 6.59e-165
Identity = 232/296 (78.38%), Postives = 254/296 (85.81%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           EVVF+F  VFRVF+SGRVERL+P D VPPSTD RTGVQSKD+LIDP T +SVRIYLP V 
Sbjct: 8   EVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVRIYLPKVS 67

Query: 61  DSTK-LPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
            S K LPLLIYIHGGAFAIGS  S+IYH HLISLAAEANAVA+SV YRLAPE+PLPAAYD
Sbjct: 68  TSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYD 127

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG---NGPAGL 180
           DAW +VEW ASH+NRDGAE+WLN HADFDRVF+VGDSAGANISYN+VGRA    NGP GL
Sbjct: 128 DAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASENDNGPEGL 187

Query: 181 NIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAEND 240
            I+GLGL+HPFFMIDKPDKLIEYIFPTS GLEDPRMNP ANP L   RC +V+I+ AE+D
Sbjct: 188 KIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNRVLIIAAEHD 247

Query: 241 WLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFIQ 292
           +L DR RGFHAALRESGWKGSVE+VE EGE HVFHLFNP+  KA  LVK VASFIQ
Sbjct: 248 FLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCGKAVALVKTVASFIQ 303

[Close]

BLAST of MC02g1001 vs. ExPASy TrEMBL
Match: A0A6A1V3B3 (Putative carboxylesterase 2 OS=Morella rubra OX=262757 GN=CJ030_MR7G011432 PE=4 SV=1)

HSP 1 Score: 354 bits (909), Expect = 1.13e-119
Identity = 170/291 (58.42%), Postives = 219/291 (75.26%), Query Frame = 0


Query: 2   VVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVPD 61
           V  +F P FRVF  G VERL+  + VPP+TD  TGVQSKD+ I  +TGVS R++LP    
Sbjct: 8   VAHDFPPFFRVFEDGTVERLLGTETVPPTTDSFTGVQSKDVTISAETGVSARLFLPKTAS 67

Query: 62  ST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDD 121
           ST KLPLLIY+HGGAF+I S  S  YH H+ SL AEAN VALS++YR APEHPLP AYDD
Sbjct: 68  STHKLPLLIYVHGGAFSIESPFSPAYHSHVSSLVAEANVVALSIHYRRAPEHPLPIAYDD 127

Query: 122 AWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN-GPAGLNIV 181
            W +++WAA+H   +G E WLN+H DF+RVF+ GDSAGANI++ +V +AG+ G  G+ IV
Sbjct: 128 TWAAMKWAATHCRCNGPEAWLNDHVDFERVFVAGDSAGANIAHVMVRKAGDDGLEGVKIV 187

Query: 182 GLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWLK 241
           G+ L+HP+F  D+PDKL++++FPT +G  D R+ P  +  L SL C +V+ILVAE DWL 
Sbjct: 188 GMVLVHPYFGNDRPDKLLDFVFPTISGPNDHRIYPNKDSNLSSLGCNRVLILVAERDWLN 247

Query: 242 DRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASF 290
           +RGR ++ AL++SGW G+VEIVETEGE HVFHLFNP  +KA  L+KQ+ASF
Sbjct: 248 ERGRTYYEALKQSGWGGTVEIVETEGEDHVFHLFNPNCDKALALLKQMASF 298

[Close]

BLAST of MC02g1001 vs. ExPASy TrEMBL
Match: A0A6P5TIN3 (probable carboxylesterase 12 OS=Prunus avium OX=42229 GN=LOC110767917 PE=4 SV=1)

HSP 1 Score: 353 bits (907), Expect = 2.43e-119
Identity = 167/293 (57.00%), Postives = 224/293 (76.45%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVP 60
           E+  EF  +FR+++ GRVERL   + VPPSTD  TGV+SKD+++ P++G+S RI+LP +P
Sbjct: 9   EISVEFPTIFRIYKDGRVERLKGTETVPPSTDLETGVRSKDVVLSPESGLSARIFLPKLP 68

Query: 61  DST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
           D T KLPLL++IHGGAF I S  S +YH H   L +EAN V LSVNYR APEHPLP AY+
Sbjct: 69  DPTRKLPLLVFIHGGAFVIESPYSPLYHNHAALLTSEANVVVLSVNYRRAPEHPLPVAYE 128

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG-NGPAGLNI 180
           D+W +++WAA+H+NR+G EIWLN++ DFDRVF+ GDSAGA +++++V RAG +G +G  I
Sbjct: 129 DSWEALQWAAAHSNRNGPEIWLNDYVDFDRVFVGGDSAGATLTHHVVHRAGVHGLSGAKI 188

Query: 181 VGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILVAENDWL 240
           VG+ L HPFF  +KPDKL+E +FP   GLEDPR+NP  +P+L  + C +V+I VAE D+L
Sbjct: 189 VGMILFHPFFGNEKPDKLLEVVFPACNGLEDPRVNPGKDPKLGEIGCGRVLIFVAEKDFL 248

Query: 241 KDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQVASFI 291
           +DR   ++ AL++SGW G VEI+E+EGE HVFHLFNP  +KA  LVK+V  FI
Sbjct: 249 RDRAWAYYEALKKSGWGGMVEILESEGEDHVFHLFNPSCDKAVGLVKKVVCFI 301

[Close]

BLAST of MC02g1001 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 8.1e-67
Identity = 136/308 (44.16%), Postives = 190/308 (61.69%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP-PV 60
           +V  E  P   V   G VERL   +  PP  D  TGV SKDI+I+P+TG+S RIY P  +
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 61  PDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYD 120
               K+PL++Y HGGAF I S     YH  L  +  +AN +A+SVNYRLAPEHPLP AY+
Sbjct: 67  QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYE 126

Query: 121 DAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIV 180
           D+WT+++   +       E W+N++AD D +FLVGDSAGANIS++L  RA      L I 
Sbjct: 127 DSWTALKNIQAI-----NEPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSDQTLKIK 186

Query: 181 GLGLIHPFFMIDKP--------------DKLIEYIFPTSAGLEDPRMNPRA--NPELRSL 240
           G+G+IHP+F   +P              D   E++ P+  G +DP +NP A  +P+L  L
Sbjct: 187 GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPDLGGL 246

Query: 241 RCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATL 292
            C +V+I VAE D L +RG+ ++  L +S WKG VEI+ET+ + HVFH+F P+ ++A  +
Sbjct: 247 GCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDEAMEM 306

[Close]

BLAST of MC02g1001 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 248.1 bits (632), Expect = 9.0e-66
Identity = 127/312 (40.71%), Postives = 187/312 (59.94%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPP-- 60
           E+ F++ P FR+F++G +ERL+P   VPPS +   GV SKD +  P+  +S+RIYLP   
Sbjct: 4   EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNS 63

Query: 61  --VPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPA 120
                  K+PLL+Y HGG F + +A S IYH  L S  +  + +A+SV YR APEHP+P 
Sbjct: 64  VYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPT 123

Query: 121 AYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN---GP 180
            Y+D+W +++W  +H  R G E WLN HADF +VFL GDSAGANI++++  R       P
Sbjct: 124 LYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPP 183

Query: 181 AGLNIVGLGLIHPFFM------------IDKPDKLIEYIFPTSA-GLEDPRMNPRANPEL 240
               I G+ L HP+F+            +   ++L     P S  G+EDP +N     +L
Sbjct: 184 ENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV-VGSDL 243

Query: 241 RSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKA 293
             L C +V+++VA ND L   G  + A L +SGW G V+++ET+ EGHVFHL +P++E A
Sbjct: 244 TGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENA 303

[Close]

BLAST of MC02g1001 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-65
Identity = 135/323 (41.80%), Postives = 198/323 (61.30%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP--- 60
           E+  ++ P+  +++SGR+ERL+    VPPS++ + GV SKD++  P   +S+RIYLP   
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 61  ---PVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPL 120
                  S KLPLL+Y HGG F + +A S  YH  L +  + ++ VA+SV+YR APEHP+
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 121 PAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGN--- 180
           P +YDD+WT+++W  SH    G+E WLN HADF +VFL GDSAGANI++++  +A     
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 181 GPAGLN---IVGLGLIHPFFMIDKP--DK---------LIEYIFPTSA-----GLEDPRM 240
            P  LN   I G+ L+HP+F    P  DK          IE ++  ++     G +DP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 241 N--PRANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSV-EIVETEGEGHV 293
           N     + +L  L C +V+++VAE D L  +G G+   L +S W G V ++VET+GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

[Close]

BLAST of MC02g1001 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-65
Identity = 135/318 (42.45%), Postives = 192/318 (60.38%), Query Frame = 0


Query: 1   EVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLP--- 60
           E+  +  P+ ++++SGR+ERL+    VPPS++ + GV SKD++      +SVRIYLP   
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 61  PVPDSTKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAA 120
                +KLPLL+Y HGG F I +A S  YH  L +  + +N VA+SV+YR APEHP+   
Sbjct: 64  AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 123

Query: 121 YDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNG--PAG 180
           +DD+WT+++W  +H    G E WLN HADF RVFL GDSAGANI +++  RA       G
Sbjct: 124 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPG 183

Query: 181 LN---IVGLGLIHPFFM----IDKPDKLIEYI-----------FPTSA-GLEDPRMN--P 240
           LN   I G+ L+HP+F     ID+ D   E +            P S  G +DP +N   
Sbjct: 184 LNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQ 243

Query: 241 RANPELRSLRCTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFN 293
             + +L  L C +V+++VAE D L  +G G+ A L +SGWKG VE+VE+EGE HVFHL  
Sbjct: 244 SESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLK 303

[Close]

BLAST of MC02g1001 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.3e-64
Identity = 130/307 (42.35%), Postives = 183/307 (59.61%), Query Frame = 0


Query: 2   VVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVRIYLPPVPD 61
           + F+  P+FRV++SGR+ERL+    VPPS   + GV SKDI+  P+  +S+RIYLP    
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 62  STKLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDDA 121
             KLP+LIY HGG F I +A S  YH  L S  A AN +A+SVNYR APE P+P  Y+D+
Sbjct: 65  VKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPYEDS 124

Query: 122 WTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNGPAGLNIV-G 181
           W S++W  +H    G E W+N H DF +VFL GDSAG NIS++L  RA       +++ G
Sbjct: 125 WDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSLISG 184

Query: 182 LGLIHPFFMIDKPDKLIE---------------YIFPTS-AGLEDPRMNPRANPELRSLR 241
           + LIHP+F    P    E                  P S  G++DP +N     +   L 
Sbjct: 185 IILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNV-VGSDPSGLG 244

Query: 242 CTQVVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLV 292
           C +V+++VA +D    +G  +   L++SGW+G VE++ET+ EGHVFHL NP ++ A  +V
Sbjct: 245 CGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNARQVV 304

The following BLAST results are available for this feature:

BLAST of MC02g1001 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SX78	1.1e-65	44.16	Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1	[more]
Q9LMA7	1.3e-64	40.71	Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1	[more]
Q9SMM9	1.7e-64	41.80	Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1	[more]
Q9SMN0	2.8e-64	42.45	Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1	[more]
I4DST8	6.3e-64	41.29	Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN...	[more]

BLAST of MC02g1001 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148541.1	5.29e-214	100.00	probable carboxylesterase 2 [Momordica charantia]	[more]
KAG6605131.1	2.88e-166	79.05	putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023007510.1	4.09e-166	78.72	probable carboxylesterase 2 [Cucurbita maxima]	[more]
KAG7035128.1	8.24e-166	78.72	putative carboxylesterase 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023532126.1	1.66e-165	78.38	probable carboxylesterase 2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC02g1001 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5P6	2.56e-214	100.00	probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 ...	[more]
A0A6J1L361	1.98e-166	78.72	probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=...	[more]
A0A6J1G929	6.59e-165	78.38	probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 S...	[more]
A0A6A1V3B3	1.13e-119	58.42	Putative carboxylesterase 2 OS=Morella rubra OX=262757 GN=CJ030_MR7G011432 PE=4 ...	[more]
A0A6P5TIN3	2.43e-119	57.00	probable carboxylesterase 12 OS=Prunus avium OX=42229 GN=LOC110767917 PE=4 SV=1	[more]

BLAST of MC02g1001 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47480.1	8.1e-67	44.16	alpha/beta-Hydrolases superfamily protein	[more]
AT1G19190.1	9.0e-66	40.71	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48700.1	1.2e-65	41.80	carboxyesterase 13	[more]
AT3G48690.1	2.0e-65	42.45	alpha/beta-Hydrolases superfamily protein	[more]
AT2G03550.1	1.3e-64	42.35	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 68..271 e-value: 8.1E-46 score: 156.5
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 14..292 e-value: 4.2E-72 score: 245.3
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 11..291
None	No IPR available	PANTHER	PTHR23024:SF414	CARBOXYLESTERASE 13-RELATED	coord: 1..291
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 1..291
IPR002168	Lipase, GDXG, putative histidine active site	PROSITE	PS01173	LIPASE_GDXG_HIS	coord: 68..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g1001.1	MC02g1001.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0052689	carboxylic ester hydrolase activity
molecular_function	GO:0016787	hydrolase activity