MC02g0940 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0940
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionisoflavone reductase homolog
LocationMC02: 7515812 .. 7518051 (-)
RNA-Seq ExpressionMC02g0940
SyntenyMC02g0940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCACACTTCCGAGTCCCATACGAATCAAAGGCAATATTTCGAATCAACGAAGAACGAAAATTCTCCCACCGTCTTCTTCTTTCGATTTCCAGCTCGCCGGTAGTTCTCCGATCAACTATTTGTTCTCCGTGAGGCTTAATCCGATGGCGCAGAAGCTCAAGGTTCTCATCATCGGAGGAACTGGTTATATCGGAAAATTTATTGTACAAGCCAGCGCCAAAGCTGGTCATCCAACCTACGCATTGCTCCGCAAATCCTCCGCCAAGTCTCGGATCATCAACAATTTCAGGAGCTTGGGCGTCAATTTTGTCTACGTAAGTTCGTTATACTCTATTTCTCTGGATTTTGTTGTTTGGTTGATCAGAATGTTCAGGAAGATTATGTGGAATCGATTGCTGAACTGTTCCCGGTTTTCTTGCATTACTAGTTTGTTCGCAATTACCTAATGTAGGTTTGTTTGCCTCAATTTGCTCGTGTTTTGTTTCTTTGAAACTAATAATGTAGCATATACTGATGATCATTTAAGTCATTCGCATCATAAATCTCTTTAATCTTGGGAATCAAGATGAATTTTCGTAGTGGAAATGACAATGTTCGATTTGCTAAAACGCTGAGAATTGAGCAGGGAGATATCTTCGACAATGAGAGTCTGGTTGAGGCTATAAAGCAGGTTGATGTGGTGATATCCACCATTGGTGGCCATATGATACCCTATCAGCATAAGATCTTATCTGCTATCAAACAAGCGGGCAATGTCAAGGTAACCTTCATTTCATCACTATTATTTCTTCACCAAACTTGTTCAATATTTTGCCATACAACTATGGCATTGATTATCGTTACTGCAATATGCGTTCTGCTAATGGATTGGAAAACTATGACAATTAAAGTTTGTATGGTGCAGAGATTCTTTCCCTCAGAGTTTGGCAACGATGCCGATCACATCCACGCGGTGGAGCCGGCGAAATCAATGTATGCTGCGAAGGCTGAATTTCGTAGGGCTGTTGAAGCAGAGGGAATCCCTCATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAGACGTTCCACCTAGAGACAAAGTCGTAATCTTAGGGGATGGAAATCCCAAAGGTATAAATTGTTAATTGCATTGTCTTGAATTCTGATTGAACTTTTTATTTTCCCGGGTTATTTTACAGCACCATTTCCCATTTCCTTGAGTTGAATTGGTTTCCAAAGTGATAAGCTTCCTCGAAATCAAATAGATATTGAAGCGTTTCATTGGATTTTTTTTAATCAAAAACTTCCAACATGTACATATATTTGTTATGTATGGTTGACCGCAACAAGAAAGATTCATTCATGATCATTTTATTGAAGGGAAATGGATTTGGCTTCAGTAGCACCTCACACTACCCATTCTGAATCTGTGTTTTTTCCTGAAGCAATTTACAACTTTTCTAGAAATACTTCTACATATGCACATGCAATATATAATTGACTGCAACATGAAGCACTGTTTCTTGAAGGGTGAAATGGATTTGGTTTCAGCAGCACCTTACACTATTCATTCTGCAGTTGTTTGTTTCCTGAAGTAGTAATGTACAAGGAAGAAAACTTTATAATAAAAAGAAGTTCTACATATACACATGTTATATATAATGGACTGCAACATGAATCATCATTCACAACACCAGCTGTTTGAAGAGAAGCACCTCTCACACAATTCACTCTGTATCTATTTTGTTTGGTGAAGTGATTTACAACAAGGAAGAAGACGTTGGAACATACACCATCCGAGCCATAGATGATCCAAGAACCCTCAACAAGATCGTGTACATTAGACCCCCAGCCAACATCTACTCGACCAACGATCTGGTCTCGCTGTGGGAAAGAAAAATCGGCAGGAGTCTTAGAAGGACCTACGTCCCAGAGGAGGAGCTGCTGAAGAAAATCCAAGGTTGGTGTGGGAAAAACAACAAAATTTCTGTTCTACCACATGTTTTCAGTTCTAATACATAGACTAACGAACACATTCATTTTTTTGCAAATGATCTTTTGCAGAGGCCTCATATCCACTTAATATAGAATTGGCACTTTGTCACACTGCTCAGGTGAAGGGAGATCAAACCAACTTTGATATTGAACCATCATTTGGAGTGGAAGCCTCAGAGCTGTACCCAGATGTTCAATACACAACTGTGGATGAATACCTCAACCAATTTGTC

mRNA sequence

TTCACACTTCCGAGTCCCATACGAATCAAAGGCAATATTTCGAATCAACGAAGAACGAAAATTCTCCCACCGTCTTCTTCTTTCGATTTCCAGCTCGCCGGTAGTTCTCCGATCAACTATTTGTTCTCCGTGAGGCTTAATCCGATGGCGCAGAAGCTCAAGGTTCTCATCATCGGAGGAACTGGTTATATCGGAAAATTTATTGTACAAGCCAGCGCCAAAGCTGGTCATCCAACCTACGCATTGCTCCGCAAATCCTCCGCCAAGTCTCGGATCATCAACAATTTCAGGAGCTTGGGCGTCAATTTTGTCTACGGAGATATCTTCGACAATGAGAGTCTGGTTGAGGCTATAAAGCAGGTTGATGTGGTGATATCCACCATTGGTGGCCATATGATACCCTATCAGCATAAGATCTTATCTGCTATCAAACAAGCGGGCAATGTCAAGAGATTCTTTCCCTCAGAGTTTGGCAACGATGCCGATCACATCCACGCGGTGGAGCCGGCGAAATCAATGTATGCTGCGAAGGCTGAATTTCGTAGGGCTGTTGAAGCAGAGGGAATCCCTCATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAGACGTTCCACCTAGAGACAAAGTCGTAATCTTAGGGGATGGAAATCCCAAAGTGATTTACAACAAGGAAGAAGACGTTGGAACATACACCATCCGAGCCATAGATGATCCAAGAACCCTCAACAAGATCGTGTACATTAGACCCCCAGCCAACATCTACTCGACCAACGATCTGGTCTCGCTGTGGGAAAGAAAAATCGGCAGGAGTCTTAGAAGGACCTACGTCCCAGAGGAGGAGCTGCTGAAGAAAATCCAAGAGGCCTCATATCCACTTAATATAGAATTGGCACTTTGTCACACTGCTCAGGTGAAGGGAGATCAAACCAACTTTGATATTGAACCATCATTTGGAGTGGAAGCCTCAGAGCTGTACCCAGATGTTCAATACACAACTGTGGATGAATACCTCAACCAATTTGTC

Coding sequence (CDS)

TTCACACTTCCGAGTCCCATACGAATCAAAGGCAATATTTCGAATCAACGAAGAACGAAAATTCTCCCACCGTCTTCTTCTTTCGATTTCCAGCTCGCCGGTAGTTCTCCGATCAACTATTTGTTCTCCGTGAGGCTTAATCCGATGGCGCAGAAGCTCAAGGTTCTCATCATCGGAGGAACTGGTTATATCGGAAAATTTATTGTACAAGCCAGCGCCAAAGCTGGTCATCCAACCTACGCATTGCTCCGCAAATCCTCCGCCAAGTCTCGGATCATCAACAATTTCAGGAGCTTGGGCGTCAATTTTGTCTACGGAGATATCTTCGACAATGAGAGTCTGGTTGAGGCTATAAAGCAGGTTGATGTGGTGATATCCACCATTGGTGGCCATATGATACCCTATCAGCATAAGATCTTATCTGCTATCAAACAAGCGGGCAATGTCAAGAGATTCTTTCCCTCAGAGTTTGGCAACGATGCCGATCACATCCACGCGGTGGAGCCGGCGAAATCAATGTATGCTGCGAAGGCTGAATTTCGTAGGGCTGTTGAAGCAGAGGGAATCCCTCATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAGACGTTCCACCTAGAGACAAAGTCGTAATCTTAGGGGATGGAAATCCCAAAGTGATTTACAACAAGGAAGAAGACGTTGGAACATACACCATCCGAGCCATAGATGATCCAAGAACCCTCAACAAGATCGTGTACATTAGACCCCCAGCCAACATCTACTCGACCAACGATCTGGTCTCGCTGTGGGAAAGAAAAATCGGCAGGAGTCTTAGAAGGACCTACGTCCCAGAGGAGGAGCTGCTGAAGAAAATCCAAGAGGCCTCATATCCACTTAATATAGAATTGGCACTTTGTCACACTGCTCAGGTGAAGGGAGATCAAACCAACTTTGATATTGAACCATCATTTGGAGTGGAAGCCTCAGAGCTGTACCCAGATGTTCAATACACAACTGTGGATGAATACCTCAACCAATTTGTC

Protein sequence

FTLPSPIRIKGNISNQRRTKILPPSSSFDFQLAGSSPINYLFSVRLNPMAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSSAKSRIINNFRSLGVNFVYGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILGDGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRTYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVDEYLNQFV
Homology
BLAST of MC02g0940 vs. ExPASy Swiss-Prot
Match: O81355 (Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC5 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.6e-125
Identity = 218/308 (70.78%), Postives = 261/308 (84.74%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MA K ++L IGGTGYIGKFIV+ASAKAG+PTY L+R++S    AKS++I NF++LGVNFV
Sbjct: 1   MASKSQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPAKSKVIENFKALGVNFV 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
            GD++D+ESLV+AIKQVDVVIST+G   +  Q KI++AIK+AGNVKRFFPSEFGND D  
Sbjct: 61  LGDLYDHESLVKAIKQVDVVISTVGHGQLADQGKIIAAIKEAGNVKRFFPSEFGNDVDRS 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
           HAVEPAKS +  KA+ RRAVEAEGIP+T+V  NFF GYFL  L+QP A   PRDKVVILG
Sbjct: 121 HAVEPAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTLNQPGASSAPRDKVVILG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DGNPK I+NKE+D+GTYTIRA+DDPRTLNK++YIRPPAN  S N+LVSLWE+KIG++L R
Sbjct: 181 DGNPKAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLER 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEE+LLK IQEA+ PLN+ L++ H   VKGD TNF+IEPSFGVEA+ LYPDV+YTTV
Sbjct: 241 IYVPEEQLLKNIQEAAVPLNVILSISHAVFVKGDHTNFEIEPSFGVEATALYPDVKYTTV 300

Query: 349 DEYLNQFV 353
           DEYLNQFV
Sbjct: 301 DEYLNQFV 308

BLAST of MC02g0940 vs. ExPASy Swiss-Prot
Match: B9HRL7 (Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=PCBER PE=1 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 5.0e-124
Identity = 213/303 (70.30%), Postives = 259/303 (85.48%), Query Frame = 0

Query: 54  KVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFVYGDIF 113
           K+L IGGTGYIGKFIV+ASAKAGHPT+ L+R+S+    AKS +I NF++LGVNF+ GD+F
Sbjct: 4   KILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIYNFKNLGVNFLIGDLF 63

Query: 114 DNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAVEP 173
           D+ESLV+AIKQVDVVIST+G   +  Q +I++AIK+AGNVKRFFPSEFGND D ++AVEP
Sbjct: 64  DHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNAVEP 123

Query: 174 AKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILGDGNPK 233
           AKS +A KA  RRA+EAEGIP+T+V  NFF GYFL + +QP A  PPRDKVVILGDGNPK
Sbjct: 124 AKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDGNPK 183

Query: 234 VIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRTYVPE 293
            ++NKE+D+ TYTI+A+DDPRTLNKI+YI+PPAN  S NDLVSLWE+KIG++L R YVPE
Sbjct: 184 AVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIYVPE 243

Query: 294 EELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVDEYLN 353
           E+LLK IQEAS P+N+ L++ H+  VKGD TNF+IEPSFGVEASELYPDV+YTTVDEYL 
Sbjct: 244 EQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDEYLK 303

BLAST of MC02g0940 vs. ExPASy Swiss-Prot
Match: Q9T030 (Phenylcoumaran benzylic ether reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PCBER1 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.6e-120
Identity = 206/308 (66.88%), Postives = 252/308 (81.82%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           M  K K+L IGGTGYIGK+IV+ASA++GHPT  L+R S+    ++S  I NF++LGV F+
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
            GD+ D+ SLV +IKQ DVVIST+G  ++ +Q+KI+SAIK+AGNVKRFFPSEFGND D +
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
             VEPAKS YA KA+ RR +EAEGIP+T+V CNFF GYFL  L+QP A   PRDKV++LG
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DGNPK ++NKEED+GTYTI A+DDPRTLNKI+YIRPP N YS NDLVSLWE KIG++L R
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEE+LLK+I E+S PLN+ L+LCH   VKG  T+F+IEPSFGVEASELYPDV+YTTV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 349 DEYLNQFV 353
           DE LNQ+V
Sbjct: 301 DEILNQYV 308

BLAST of MC02g0940 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.7e-119
Identity = 206/309 (66.67%), Postives = 259/309 (83.82%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           M  K K+LIIGGTGYIGKFIV+AS K GHPT+AL+R+++     K +++  F++LGV+ +
Sbjct: 1   MGSKSKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           YGD++D++SLV+AIKQVDVVIST+G   I  Q KI++AIK+AGNVKRFFPSEFGND DH+
Sbjct: 61  YGDLYDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHV 120

Query: 169 HAVEPAKSM-YAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVIL 228
           +AVEPAKS+ +A KA  RRAVEAEGIP+T+V  N F+GYFL  L QP A  PPRDKV+I 
Sbjct: 121 NAVEPAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIP 180

Query: 229 GDGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLR 288
           GDGNPK I+NKEED+GTYTI+A+DDPRTLNKI+Y+RP  NIYS N+LV+LWE+KIG++L 
Sbjct: 181 GDGNPKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLE 240

Query: 289 RTYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTT 348
           + YVPEE++LK IQEA  P+NI L + H+  VKGD TNF+IEPSFGVEASELYP+V+YTT
Sbjct: 241 KIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTT 300

Query: 349 VDEYLNQFV 353
           V+EYL+QFV
Sbjct: 301 VEEYLDQFV 309

BLAST of MC02g0940 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 4.1e-118
Identity = 201/308 (65.26%), Postives = 254/308 (82.47%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MA K K+LIIGGTGYIGKFIV+ASAK+GHPT+AL+R+S+     K +++  F+ LGV  +
Sbjct: 1   MAHKSKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           +GD++D+ESLV+A KQVDVVIST+G   +  Q KI++AIK+AGN+KRFFPSEFGND D +
Sbjct: 61  HGDLYDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRV 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
           HAVEPAK+ +A KAE RR  EAEGIP+T+V  NFF GYFL  L+QP    PPR+KVVI G
Sbjct: 121 HAVEPAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DGN + ++NKE+D+GTYTIRA+DDPRTLNKIVYI+P  NIYS N++V+LWE+KIG++L +
Sbjct: 181 DGNARAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEK 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEE+LLK IQE+  P+N+ LA+ H+  VKGD TNF+IE SFGVEASELYPDV+YTTV
Sbjct: 241 IYVPEEKLLKDIQESPIPINVILAINHSVFVKGDHTNFEIEASFGVEASELYPDVKYTTV 300

Query: 349 DEYLNQFV 353
           +EYL QFV
Sbjct: 301 EEYLQQFV 308

BLAST of MC02g0940 vs. NCBI nr
Match: XP_022146291.1 (isoflavone reductase homolog [Momordica charantia])

HSP 1 Score: 610 bits (1572), Expect = 3.14e-219
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSSAKSRIINNFRSLGVNFVYGDI 108
           MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSSAKSRIINNFRSLGVNFVYGDI
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSSAKSRIINNFRSLGVNFVYGDI 60

Query: 109 FDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAVE 168
           FDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAVE
Sbjct: 61  FDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAVE 120

Query: 169 PAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILGDGNP 228
           PAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILGDGNP
Sbjct: 121 PAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILGDGNP 180

Query: 229 KVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRTYVP 288
           KVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRTYVP
Sbjct: 181 KVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRTYVP 240

Query: 289 EEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVDEYL 348
           EEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVDEYL
Sbjct: 241 EEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVDEYL 300

Query: 349 NQFV 352
           NQFV
Sbjct: 301 NQFV 304

BLAST of MC02g0940 vs. NCBI nr
Match: KAG7010928.1 (hypothetical protein SDJN02_27726 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 586 bits (1510), Expect = 5.14e-205
Identity = 290/337 (86.05%), Postives = 312/337 (92.58%), Query Frame = 0

Query: 20  KILPPSSSFDFQLAGSSPINYLFSVRLNPMAQKLKVLIIGGTGYIGKFIVQASAKAGHPT 79
           + LP SSSF  QLAGSSPI Y F+     MAQ LKVLIIGGTGYIGKFIVQASAKAGHPT
Sbjct: 275 RTLPVSSSFFLQLAGSSPIRYFFAGNFTSMAQNLKVLIIGGTGYIGKFIVQASAKAGHPT 334

Query: 80  YALLRKSS----AKSRIINNFRSLGVNFVYGDIFDNESLVEAIKQVDVVISTIGGHMIPY 139
           YAL+RKSS    AKS+IIN+F+SLGVNF++GD+FDNESLV+AIKQVDVVIST+GGHM+P+
Sbjct: 335 YALIRKSSLGSSAKSQIINHFKSLGVNFLFGDLFDNESLVKAIKQVDVVISTLGGHMVPH 394

Query: 140 QHKILSAIKQAGNVKRFFPSEFGNDADHIHAVEPAKSMYAAKAEFRRAVEAEGIPHTFVV 199
           QHKILSAIKQAGNVKRFFP+EFGNDADHI AVEPAKSMY AKAEFRR VEAEGIPHTFVV
Sbjct: 395 QHKILSAIKQAGNVKRFFPTEFGNDADHIDAVEPAKSMYTAKAEFRRVVEAEGIPHTFVV 454

Query: 200 CNFFDGYFLSNLSQPDADVPPRDKVVILGDGNPKVIYNKEEDVGTYTIRAIDDPRTLNKI 259
           CNFFDGYFLSNLSQPDA VPPRDKVVILGDG PKVIYNKEEDVGTYTIRAIDDPRTLNKI
Sbjct: 455 CNFFDGYFLSNLSQPDASVPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKI 514

Query: 260 VYIRPPANIYSTNDLVSLWERKIGRSLRRTYVPEEELLKKIQEASYPLNIELALCHTAQV 319
           +YIRPPANIYSTNDLVSLWERKIG++LRR YVPEEE+LK IQEASYPLNIELALCHTAQV
Sbjct: 515 MYIRPPANIYSTNDLVSLWERKIGKTLRRVYVPEEEVLKNIQEASYPLNIELALCHTAQV 574

Query: 320 KGDQTNFDIEPSFGVEASELYPDVQYTTVDEYLNQFV 352
           +G QTNFDIEPSFGVEAS LYPDV+YTTV+EYL+QFV
Sbjct: 575 RGSQTNFDIEPSFGVEASALYPDVRYTTVEEYLDQFV 611

BLAST of MC02g0940 vs. NCBI nr
Match: KAG6571107.1 (Phenylcoumaran benzylic ether reductase Pyrc5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 557 bits (1435), Expect = 2.73e-198
Identity = 274/308 (88.96%), Postives = 294/308 (95.45%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MAQ LKVLIIGGTGYIGKFIVQASAKAGHPTYAL+RKSS    AKS+IIN+F+SLGVNF+
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           +GD+FDNESLV+AIKQVDVVIST+GGHM+P+QHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
            AVEPAKSMY AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDA VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DG PKVIYNKEEDVGTYTIRAIDDPRTLNKI+YIRPPANIYSTNDLVSLWERKIG++LRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPPANIYSTNDLVSLWERKIGKTLRR 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEEE+LK IQEASYPLNIELALCHTAQV+G QTNFDIEPSFGVEAS LYPDV+YTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 349 DEYLNQFV 352
           +EYL+QFV
Sbjct: 301 EEYLDQFV 308

BLAST of MC02g0940 vs. NCBI nr
Match: XP_022943555.1 (isoflavone reductase-like protein [Cucurbita moschata])

HSP 1 Score: 555 bits (1429), Expect = 2.24e-197
Identity = 273/308 (88.64%), Postives = 293/308 (95.13%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MAQ LKVLIIGGTGYIGKFIVQASAKAGHPTYAL+RKSS    AKS+IIN+F+SLGV F+
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVKFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           +GD+FDNESLV+AIKQVDVVIST+GGHM+P+QHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
            AVEPAKSMY AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDA VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DG PKVIYNKEEDVGTYTIRAIDDPRTLNKI+YIRPPANIYSTNDLVSLWERKIG++LRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPPANIYSTNDLVSLWERKIGKTLRR 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEEE+LK IQEASYPLNIELALCHTAQV+G QTNFDIEPSFGVEAS LYPDV+YTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 349 DEYLNQFV 352
           +EYL+QFV
Sbjct: 301 EEYLDQFV 308

BLAST of MC02g0940 vs. NCBI nr
Match: XP_022986575.1 (isoflavone reductase-like protein [Cucurbita maxima] >XP_023512224.1 isoflavone reductase homolog PCBER-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 554 bits (1427), Expect = 4.51e-197
Identity = 273/308 (88.64%), Postives = 293/308 (95.13%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MAQ LKVLIIGGTGYIGKFIVQASAKAGHPTYAL+RKSS    AKS+IIN+F+SLGVNF+
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           +GD+FDNESLV+AIKQVDVVIST+GGHM+P+QHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
            AVEPAKSMY AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDA VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DG PKVIYNKEEDVGTYTIRAIDDPRTLNKI+YIRP ANIYSTNDLVSLWERKIG++LRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEEE+LK IQEASYPLNIELALCHTAQV+G QTNFDIEPSFGVEAS LYPDV+YTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 349 DEYLNQFV 352
           +EYL+QFV
Sbjct: 301 EEYLDQFV 308

BLAST of MC02g0940 vs. ExPASy TrEMBL
Match: A0A6J1CYV4 (isoflavone reductase homolog OS=Momordica charantia OX=3673 GN=LOC111015535 PE=4 SV=1)

HSP 1 Score: 610 bits (1572), Expect = 1.52e-219
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSSAKSRIINNFRSLGVNFVYGDI 108
           MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSSAKSRIINNFRSLGVNFVYGDI
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSSAKSRIINNFRSLGVNFVYGDI 60

Query: 109 FDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAVE 168
           FDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAVE
Sbjct: 61  FDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAVE 120

Query: 169 PAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILGDGNP 228
           PAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILGDGNP
Sbjct: 121 PAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILGDGNP 180

Query: 229 KVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRTYVP 288
           KVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRTYVP
Sbjct: 181 KVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRTYVP 240

Query: 289 EEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVDEYL 348
           EEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVDEYL
Sbjct: 241 EEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVDEYL 300

Query: 349 NQFV 352
           NQFV
Sbjct: 301 NQFV 304

BLAST of MC02g0940 vs. ExPASy TrEMBL
Match: A0A6J1FXY6 (isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448291 PE=4 SV=1)

HSP 1 Score: 555 bits (1429), Expect = 1.08e-197
Identity = 273/308 (88.64%), Postives = 293/308 (95.13%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MAQ LKVLIIGGTGYIGKFIVQASAKAGHPTYAL+RKSS    AKS+IIN+F+SLGV F+
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVKFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           +GD+FDNESLV+AIKQVDVVIST+GGHM+P+QHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
            AVEPAKSMY AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDA VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DG PKVIYNKEEDVGTYTIRAIDDPRTLNKI+YIRPPANIYSTNDLVSLWERKIG++LRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPPANIYSTNDLVSLWERKIGKTLRR 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEEE+LK IQEASYPLNIELALCHTAQV+G QTNFDIEPSFGVEAS LYPDV+YTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 349 DEYLNQFV 352
           +EYL+QFV
Sbjct: 301 EEYLDQFV 308

BLAST of MC02g0940 vs. ExPASy TrEMBL
Match: A0A6J1JEF2 (isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111484277 PE=4 SV=1)

HSP 1 Score: 554 bits (1427), Expect = 2.18e-197
Identity = 273/308 (88.64%), Postives = 293/308 (95.13%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MAQ LKVLIIGGTGYIGKFIVQASAKAGHPTYAL+RKSS    AKS+IIN+F+SLGVNF+
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVNFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           +GD+FDNESLV+AIKQVDVVIST+GGHM+P+QHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
            AVEPAKSMY AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDA VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DG PKVIYNKEEDVGTYTIRAIDDPRTLNKI+YIRP ANIYSTNDLVSLWERKIG++LRR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPAANIYSTNDLVSLWERKIGKTLRR 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEEE+LK IQEASYPLNIELALCHTAQV+G QTNFDIEPSFGVEAS LYPDV+YTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 349 DEYLNQFV 352
           +EYL+QFV
Sbjct: 301 EEYLDQFV 308

BLAST of MC02g0940 vs. ExPASy TrEMBL
Match: A0A1S3CE33 (isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103499680 PE=4 SV=1)

HSP 1 Score: 551 bits (1419), Expect = 3.36e-196
Identity = 272/308 (88.31%), Postives = 295/308 (95.78%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MAQK  VLIIGGTGYIGKFIVQASAKAGHPTYAL+R+SS    AK+RI+N+F+SLGVNF+
Sbjct: 1   MAQK--VLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           +GD+FDNESLV+AIKQVDVVIST+GGHM+P+QHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
            AVEPAKSMY+AKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDA VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DG PKVIYNKEEDVGTYTIRAIDDPRTLNKI+Y+RPPANIYSTNDLVSLWERKIG+SL+R
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEEE+LKKI+E SYPLNIELALCHTAQVKG QTNFDIEPSFGVEAS LYPDV+YTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 349 DEYLNQFV 352
           +EYL+QFV
Sbjct: 301 EEYLDQFV 306

BLAST of MC02g0940 vs. ExPASy TrEMBL
Match: A0A0A0LJ01 (NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G248130 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 3.91e-195
Identity = 271/308 (87.99%), Postives = 294/308 (95.45%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           MAQK  VLIIGGTGYIGKFIVQASAKAGHPTYAL+R+SS    AK+RI+N+F+SLGVNF+
Sbjct: 1   MAQK--VLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
           +GD+FDNESLV+AIKQVDVVIST+GGHM+P+QHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
            AVEPAKSMYAAKAEFRRA+EAEGIPHTFVVCNFFDGYFLSNLSQPDA VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DG PKVIYNKEEDVGTYTIRAIDDPRTLNKI+Y+RPPANIYSTNDLVSLWERKIG+SL+R
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEEE+LKKI+E SYPLNIELALCHTAQVKG QTNF IEPSFGVEAS LYPDV+YTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300

Query: 349 DEYLNQFV 352
           +EYL+QFV
Sbjct: 301 EEYLDQFV 306

BLAST of MC02g0940 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 433.3 bits (1113), Expect = 1.8e-121
Identity = 206/308 (66.88%), Postives = 252/308 (81.82%), Query Frame = 0

Query: 49  MAQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFV 108
           M  K K+L IGGTGYIGK+IV+ASA++GHPT  L+R S+    ++S  I NF++LGV F+
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 109 YGDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHI 168
            GD+ D+ SLV +IKQ DVVIST+G  ++ +Q+KI+SAIK+AGNVKRFFPSEFGND D +
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 169 HAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADVPPRDKVVILG 228
             VEPAKS YA KA+ RR +EAEGIP+T+V CNFF GYFL  L+QP A   PRDKV++LG
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 229 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 288
           DGNPK ++NKEED+GTYTI A+DDPRTLNKI+YIRPP N YS NDLVSLWE KIG++L R
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 289 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 348
            YVPEE+LLK+I E+S PLN+ L+LCH   VKG  T+F+IEPSFGVEASELYPDV+YTTV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 349 DEYLNQFV 353
           DE LNQ+V
Sbjct: 301 DEILNQYV 308

BLAST of MC02g0940 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 378.6 bits (971), Expect = 5.3e-105
Identity = 186/307 (60.59%), Postives = 237/307 (77.20%), Query Frame = 0

Query: 51  QKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFVYG 110
           +K K+L+IGGTGYIGKF+V+ASAKAGH T+AL+R+++     K + + +F+ LGV  ++G
Sbjct: 4   EKSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHG 63

Query: 111 DIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHA 170
           D+ D+ESLV+AIKQVDVVIST+G   I  Q KI+SAIK+AGNVKRF PSEFG D D   A
Sbjct: 64  DLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSA 123

Query: 171 VEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQ--PDADVPPRDKVVILG 230
           VEPAKS +A K + RR +EAEGIP+T+ V   F GY+L  L Q  P    PPRDKV ILG
Sbjct: 124 VEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILG 183

Query: 231 DGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRR 290
           DGN K + NKEED+  YTI+A+DDPRTLNKI+YI+P  N  S N++V+LWE+KIG+SL +
Sbjct: 184 DGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEK 243

Query: 291 TYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTV 350
           T++PEE+LLK IQE+  P+N+ L++ H   V GD TN  IEPSFGVEASELYPDV+YT+V
Sbjct: 244 THLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKYTSV 303

Query: 351 DEYLNQF 352
           DEYL+ F
Sbjct: 304 DEYLSYF 309

BLAST of MC02g0940 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 369.8 bits (948), Expect = 2.5e-102
Identity = 178/308 (57.79%), Postives = 231/308 (75.00%), Query Frame = 0

Query: 50  AQKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSSA----KSRIINNFRSLGVNFVY 109
           ++K K+L+IGGTG+IGK I++AS KAGH T AL+R++S     K + + NF+  GV  ++
Sbjct: 3   SEKSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLH 62

Query: 110 GDIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIH 169
           GD+ D+ESLV+AIKQ DVVIST+G   I  Q KI+SAIK+AGNVKRF PSEFG D D   
Sbjct: 63  GDLNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSS 122

Query: 170 AVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQ--PDADVPPRDKVVIL 229
           AVEPAKS +  K + RR +EAEGIP+T++V N+F GY+L  L Q  P    PPRDKV I 
Sbjct: 123 AVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIF 182

Query: 230 GDGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLR 289
           GDGN K + NKEED+  YTI+A+DDPRTLNK +YI PP N  S N++V+LWE+KIG+S+ 
Sbjct: 183 GDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVE 242

Query: 290 RTYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTT 349
           + Y+ EE++ K IQE+  P N+ L++ H   VKGDQTNF IEPSFG EASELYPD++YT+
Sbjct: 243 KIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTS 302

Query: 350 VDEYLNQF 352
           +DEYL+ F
Sbjct: 303 IDEYLSYF 310

BLAST of MC02g0940 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.6e-99
Identity = 183/321 (57.01%), Postives = 239/321 (74.45%), Query Frame = 0

Query: 51  QKLKVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFVYG 110
           +K K+L+IGGTGY+G+FIV+ SAKAG+PT+AL+R++S     KS+ I +F+ LGV  ++G
Sbjct: 4   EKSKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHG 63

Query: 111 DIFDNESLVEAIKQVDVVISTIGGHMIPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHA 170
           D+ D+ESLV+AIKQVDVVISTIG   I  Q KI+SAIK+AGNVKRF P+EFG D +   A
Sbjct: 64  DLNDHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSA 123

Query: 171 VEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQ----------------P 230
           VEPAKS++A K + RRA+EAEGIP+T+VV N   G++L  L Q                 
Sbjct: 124 VEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFG 183

Query: 231 DADVPPRDKVVILGDGNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDL 290
           D +VPPRDKV ILGDGN KV+ NKEEDV  Y I+A+DD RTLNK +YI PP NI S N++
Sbjct: 184 DKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEM 243

Query: 291 VSLWERKIGRSLRRTYVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGV 350
           V+LWE+KIG+SL +T++ EE++LK IQ    P+++  ++ H   VKGDQT+F IEP FG 
Sbjct: 244 VTLWEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGE 303

Query: 351 EASELYPDVQYTTVDEYLNQF 352
           EAS LYPDV+YT++DEYL+QF
Sbjct: 304 EASVLYPDVKYTSIDEYLSQF 321

BLAST of MC02g0940 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.0e-91
Identity = 170/307 (55.37%), Postives = 220/307 (71.66%), Query Frame = 0

Query: 54  KVLIIGGTGYIGKFIVQASAKAGHPTYALLRKSS----AKSRIINNFRSLGVNFVYGDIF 113
           K+L+IG TG IGK +V+ SAK+GH T+AL+R++S     K++++  F+ LGV  +YG + 
Sbjct: 4   KILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSLS 63

Query: 114 DNESLVEAIKQVDVVISTIGGHM--IPYQHKILSAIKQAGNVKRFFPSEFGNDADHIHAV 173
           D ESLV+AIKQVDVVIS +G     I  Q  I+ AIK++GNVKRF PSEFGND D   A+
Sbjct: 64  DKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVAI 123

Query: 174 EPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDADV--PPRDKVVILGD 233
           EP  S +  KA+ RRA+EA  IP+T+VV   F G F+  L Q    +  PPRDKV I   
Sbjct: 124 EPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYDT 183

Query: 234 GNPKVIYNKEEDVGTYTIRAIDDPRTLNKIVYIRPPANIYSTNDLVSLWERKIGRSLRRT 293
           GN K I N EED+  YT++A+DDPRTLNKI+YI PP  I S ND+V LWE KIG++L +T
Sbjct: 184 GNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEKT 243

Query: 294 YVPEEELLKKIQEASYPLNIELALCHTAQVKGDQTNFDIEPSFGVEASELYPDVQYTTVD 353
           YV EEELLK IQE+  P++  + L HT  VK D T+F I+PSFGVEASELYP+V+YT+VD
Sbjct: 244 YVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSVD 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O813551.6e-12570.78Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC... [more]
B9HRL75.0e-12470.30Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=P... [more]
Q9T0302.6e-12066.88Phenylcoumaran benzylic ether reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PCB... [more]
B2WSN01.7e-11966.67Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1[more]
Q9FUW64.1e-11865.26Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6... [more]
Match NameE-valueIdentityDescription
XP_022146291.13.14e-219100.00isoflavone reductase homolog [Momordica charantia][more]
KAG7010928.15.14e-20586.05hypothetical protein SDJN02_27726 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6571107.12.73e-19888.96Phenylcoumaran benzylic ether reductase Pyrc5, partial [Cucurbita argyrosperma s... [more]
XP_022943555.12.24e-19788.64isoflavone reductase-like protein [Cucurbita moschata][more]
XP_022986575.14.51e-19788.64isoflavone reductase-like protein [Cucurbita maxima] >XP_023512224.1 isoflavone ... [more]
Match NameE-valueIdentityDescription
A0A6J1CYV41.52e-219100.00isoflavone reductase homolog OS=Momordica charantia OX=3673 GN=LOC111015535 PE=4... [more]
A0A6J1FXY61.08e-19788.64isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448291 ... [more]
A0A6J1JEF22.18e-19788.64isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111484277 PE... [more]
A0A1S3CE333.36e-19688.31isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103499680 PE=4 S... [more]
A0A0A0LJ013.91e-19587.99NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G248130 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT4G39230.11.8e-12166.88NmrA-like negative transcriptional regulator family protein [more]
AT1G75280.15.3e-10560.59NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.12.5e-10257.79NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75300.12.6e-9957.01NmrA-like negative transcriptional regulator family protein [more]
AT1G19540.12.0e-9155.37NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 55..286
e-value: 3.5E-77
score: 259.0
NoneNo IPR availableGENE3D3.90.25.10coord: 198..348
e-value: 2.4E-134
score: 449.1
NoneNo IPR availableGENE3D3.40.50.720coord: 54..338
e-value: 2.4E-134
score: 449.1
NoneNo IPR availablePANTHERPTHR43349:SF35PHENYLCOUMARAN BENZYLIC ETHER REDUCTASE 1coord: 49..352
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 49..352
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 54..347
e-value: 4.17063E-92
score: 274.951
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 54..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0940.1MC02g0940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0016491 oxidoreductase activity