MC02g0866 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g0866 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.4e-154
Identity = 303/514 (58.95%), Postives = 379/514 (73.74%), Query Frame = 0

Query: 7   SLYTSLLLGLQAILSSGSCS---FETDPSIEEATVNDLRHAFYQNKLTSKQLVKFYLEQV 66
           SL   L+L    I+S GS S     +  SI+EAT+ D+R AF + +LTSKQLV+ YLE +
Sbjct: 11  SLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAI 70

Query: 67  RRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKLNTTA 126
            + NPIL  VIE NPDAL QA  AD ER       L   HG+PVLLKD+I+TKDKLNTTA
Sbjct: 71  SKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTA 130

Query: 127 GSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGKLCSF 186
           GSFALLGSVV RDAGVV +LR++GA+I GKASLSEW+ FRS++ P+GWSARG QGK    
Sbjct: 131 GSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGK---- 190

Query: 187 SNNPYTL-GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSR 246
             NPY L   P GSSSGSAISV AN+V VSLGTETDGSIL P++ NSVVGIKP+VGLTSR
Sbjct: 191 --NPYVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSR 250

Query: 247 AGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFLRADG 306
           AGVVPISLRQD++GPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL   G
Sbjct: 251 AGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSG 310

Query: 307 LQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVIT-GSSSGE 366
           L+GKR+GIV K          +    +  +KTL++ GAI++ NLTI ++ VI  G+ SGE
Sbjct: 311 LKGKRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGE 370

Query: 367 WTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATKGI 426
             ALL EFK+SLNAYLKELV SP+ SL+  I +N+  ++ EK+KE+GQ++FL AEAT G+
Sbjct: 371 EIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGM 430

Query: 427 RVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQG 486
              EK AL ++++LS+ G E+++ +NKLDAI   G  +S VLAIGG+PG++VPAGY+  G
Sbjct: 431 GEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGG 490

Query: 487 VPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPP 516
           VPYGI FGGL+  EP+LIEIA+ FE     R+PP
Sbjct: 491 VPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of MC02g0866 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 3.4e-76
Identity = 197/515 (38.25%), Postives = 286/515 (55.53%), Query Frame = 0

Query: 32  SIEEATVNDLRHAFYQNKLTSKQLVKFYLEQVRRFNPILKGVIELNPDALHQASRADYER 91
           ++E+AT++ L++      LTS  +V  YL++  + NP + G+++LNPD L  AS  D ER
Sbjct: 65  TLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDER 124

Query: 92  KRNAPASLSPSHGIPVLLKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIF 151
                    P HGIP ++KDN ATKDK++TTAGS+ALLGS+VPRDA VV +LR+AGA++F
Sbjct: 125 ANG--IIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLF 184

Query: 152 GKASLSEWSDFRSYAPPNGWSARGGQGKLCSFSNNPYTLGEPCGSSSGSAISVAANMVTV 211
           G A+LSEW+D RS     G+SARGGQ + C F+        P GSSSGSAISVA+NM+  
Sbjct: 185 GHATLSEWADMRSNDYSEGYSARGGQSR-CPFN----LTVNPGGSSSGSAISVASNMIAF 244

Query: 212 SLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDA 271
           +LGTETDGSI+ P+  N VVG+KPTVGLTSR GV+P S  QDT GPI RTV DA YV  +
Sbjct: 245 ALGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQS 304

Query: 272 IVGADSYDISTTEASKYIPK-GGYGQFL-RADGLQGKRIGIV-RKFYDFGHDDAFYPQAF 331
           + G D  DI T   +   P+ G Y +FL     L+G R G+  ++ +     D       
Sbjct: 305 MWGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEI--DRL 364

Query: 332 EKVLKTLKQGGAILVENLTIDSLHVITGS---------SSGEWTALLVEFKISLNAYLKE 391
            +V+K +++ GAI+  N    +L VI+           +  E+T + V+F  ++ +YL E
Sbjct: 365 LEVVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSE 424

Query: 392 LVVSPIXSLSYAIEFNKRNSKLEKIK-------EYGQDLFLKAEATKGIR-VAEKAALAR 451
           +  + I SL   +E+N +    E  K         GQD FL +    G++      A+  
Sbjct: 425 VKNTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEY 484

Query: 452 LEKLSKG-GFERVM---IKNKLDAIAAPGRLISPVLAI-------GGFPGVSVPAGYNPQ 511
           + + S+  G +  +        D+    G L+    +I        G+P +++P G    
Sbjct: 485 VRRTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTN 544

Query: 512 GVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPP 516
           G P+G+G       EP+LI+     E L + +  P
Sbjct: 545 GRPFGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKP 570

BLAST of MC02g0866 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 4.4e-68
Identity = 193/536 (36.01%), Postives = 274/536 (51.12%), Query Frame = 0

Query: 31  PSIEEATVNDLRHAFYQNKLTSKQLVKF---------------------YLEQVRRFNPI 90
           P + EAT + L+    +   TS  LVK                      Y+ ++   N  
Sbjct: 63  PKLIEATADQLQDGLTKGCFTSVDLVKVRITLQTPYRQGNVLIIVVVQTYVARIAEVNST 122

Query: 91  LKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKLNTTAGSFALL 150
           ++ V E+NPDAL  A + D ERK        P HG+P+++K+NI T DK+++TAGS+A+ 
Sbjct: 123 VRAVTEINPDALTIAKQMDNERKMG--KLRGPLHGLPIVIKNNIFTDDKMSSTAGSYAIF 182

Query: 151 GSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGKLCSFSNNPYT 210
           G+    DA V TKLR+AG +I GK+  S+W++FRS    NGWSA GGQ       N    
Sbjct: 183 GARTSADATVATKLREAGLVIMGKSGASQWANFRSLNSTNGWSAYGGQVTAAYIKNQ--- 242

Query: 211 LGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPIS 270
             +P GSSSGS ++    +   +LGTET GSI+ P+  +++VG+KPTVGLTSR  VVPIS
Sbjct: 243 --DPSGSSSGSGVASDLGLAFATLGTETSGSIVSPADKSNIVGLKPTVGLTSRRFVVPIS 302

Query: 271 LRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFLRADGLQGKRIG 330
            RQDTVGP+ R+V DAAY+L  I G DS D + T A  +     Y +    + L+GKRIG
Sbjct: 303 ERQDTVGPMARSVKDAAYLLQVIAGKDSND-NYTSAIPFDTIPDYVKACDINALKGKRIG 362

Query: 331 IVRKFYD-FGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGSSSGEWTALLVE 390
           + R     FG       Q F + L  +K+ GAI+VEN    S      S   +   L  +
Sbjct: 363 VPRNVIKIFGSPQTVVDQ-FNQALAVMKKAGAIIVENTDFTSFAEFAQSPIPD-DILYAD 422

Query: 391 FKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQ------DLFLKAEATKGIR 450
              +L A+ K+L V+P       +E  +R ++  +++EY        D+ L+    KGI+
Sbjct: 423 SLTNLPAFFKQLKVNPHNITD--LESLRRFTQHHRLEEYPSRDTARWDIALQ----KGIK 482

Query: 451 VAEKAALARLEKLSK----GGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYN 510
             +       +K  K    GG    + ++KLDA   P  L   + A+ G P ++VP G  
Sbjct: 483 NTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLSPYIPALIGSPIITVPMGVY 542

Query: 511 PQ---------------GVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 520
           P                G+P GIGF G    E +LI +AY FE   K+   P LKR
Sbjct: 543 PNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYAFEQ--KTHARPKLKR 580

BLAST of MC02g0866 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 7.3e-39
Identity = 143/510 (28.04%), Postives = 243/510 (47.65%), Query Frame = 0

Query: 37  TVNDLRHAFYQNKLTSKQLVKFYLEQVRRFNPILKGVIELNPD-ALHQASRADYERKRNA 96
           T+++LR    + ++++ ++ K YLE+++   P +  ++ +  D AL +A  AD + K+  
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKKGE 66

Query: 97  PASLSPSHGIPVLLKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKAS 156
             +L+   GIPV++KDNI+T + + TT  S  L   + P +A VV KL + G II GK++
Sbjct: 67  DTALT---GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSN 126

Query: 157 LSEWSDFRSYAPPNGWSARGGQGKLCSF--SNNPYTLGE-PCGSSSGSAISVAANMVTVS 216
           L E+             A G   +  +F  + NP+ L   P GSS GSA ++AA+    +
Sbjct: 127 LDEF-------------AMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFA 186

Query: 217 LGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAI 276
           LG++T GSI  P++L  VVG+KPT GL SR G+V  +   D +GP  + V+D A VL+ I
Sbjct: 187 LGSDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTI 246

Query: 277 VGADSYDISTTEASKYIPKGGYGQFLRADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVL 336
           +G D  D      S  I K  Y  +L+ D ++G RIG+ ++F+  G ++    +  ++ +
Sbjct: 247 IGHDPKD----STSLKIDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEG-VKETVQESI 306

Query: 337 KTLKQGGAILVE------NLTIDSLHVITGSSSGEWTALLVEFKISLNAYLKELVVSPIX 396
           K L+  GA +++         + + ++I  + +    A     +    A   E ++  + 
Sbjct: 307 KVLQDLGAEIIDISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAEKYEDLID-MY 366

Query: 397 SLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATKGIRVAEKAALARLEKLSKGGFERVMIK 456
            ++ +  F K   +   +  Y          + G   A      ++  L K  FE+    
Sbjct: 367 MVTRSEGFGKEVKRRIMLGTYA--------LSSGYYDAYYKKALKVRTLIKNDFEKAF-- 426

Query: 457 NKLDAIAAP---------GRLISPVLA------------IGGFPGVSVPAGYNPQGVPYG 516
            K D I  P         G   +  LA            I G PG+S+P G +  G+P G
Sbjct: 427 EKCDVIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVG 481

BLAST of MC02g0866 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 9.5e-39
Identity = 144/510 (28.24%), Postives = 242/510 (47.45%), Query Frame = 0

Query: 37  TVNDLRHAFYQNKLTSKQLVKFYLEQVRRFNPILKGVIELNPD-ALHQASRADYERKRNA 96
           T+++LR    + ++++ ++ K YLE+++   P +  +I +  D AL +A  AD + K   
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEADEKIKNGE 66

Query: 97  PASLSPSHGIPVLLKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKAS 156
             +L+   GIPV++KDNI+T + + TT  S  L   + P +A VV KL + G II GK++
Sbjct: 67  DTALT---GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSN 126

Query: 157 LSEWSDFRSYAPPNGWSARGGQGKLCSF--SNNPYTLGE-PCGSSSGSAISVAANMVTVS 216
           L E+             A G   +  +F  + NP+ L   P GSS GSA ++AA+    +
Sbjct: 127 LDEF-------------AMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFA 186

Query: 217 LGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAI 276
           LG++T GSI  P++L  VVG+KPT GL SR G+V  +   D +GP  + V+D A VL+ I
Sbjct: 187 LGSDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTI 246

Query: 277 VGADSYDISTTEASKYIPKGGYGQFLRADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVL 336
           +G D  D      S  I K  Y  +L+ D ++G RIG+ ++F+  G ++    +  ++ +
Sbjct: 247 IGHDPKD----STSLKIDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEG-VKETVQESI 306

Query: 337 KTLKQGGAILVE------NLTIDSLHVITGSSSGEWTALLVEFKISLNAYLKELVVSPIX 396
           K L+  GA +++         + + ++I  + +    A     +    A   E ++  + 
Sbjct: 307 KVLQDLGAEIIDISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAGKYEDLID-MY 366

Query: 397 SLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATKGIRVAEKAALARLEKLSKGGFERVMIK 456
            ++ +  F K   +   +  Y          + G   A      ++  L K  FE+    
Sbjct: 367 MVTRSEGFGKEVKRRIMLGTYA--------LSSGYYDAYYKKALKVRTLIKNDFEKAF-- 426

Query: 457 NKLDAIAAP---------GRLISPVLA------------IGGFPGVSVPAGYNPQGVPYG 516
            K D I  P         G   +  LA            I G PG+S+P G +  G+P G
Sbjct: 427 EKCDVIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVG 481

BLAST of MC02g0866 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 839 bits (2168), Expect = 1.03e-303
Identity = 434/520 (83.46%), Postives = 470/520 (90.38%), Query Frame = 0

Query: 1   VAAPSFSLYTSLLLGLQAILSS-GSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVKFY 60
           +AA SF +Y S+LLGL AILSS GSCSF+T+ SIEEAT+ DL+ AFYQNKLTS QLV+FY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LEQVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKL 120
           LEQVRRFNPIL G+IE+NPDAL+QAS+AD ERKR++P SLSP HGIPVL+KDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSY  PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 LCSFSNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 240
                 NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL
Sbjct: 181 ------NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 240

Query: 241 TSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFLR 300
           TSRAGVVPISLRQDTVGPICRTV+DAAYVLDAI G D YD ST EASKY+PKGGYGQFL+
Sbjct: 241 TSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLK 300

Query: 301 ADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGSSS 360
            DGL+GKRIGIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+S HVITGSSS
Sbjct: 301 EDGLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSS 360

Query: 361 GEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATK 420
           GEWTA+L EFKIS+N YLK+LV SPI SLS AIEFN++NSKLEK+KEYGQ+LFL+AEATK
Sbjct: 361 GEWTAVLAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATK 420

Query: 421 GIRVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNP 480
           GI  AEKAALARL KLSK GFER+MIKNKLDAIAAPGRLIS  LAIGGFPGVSVPAGYNP
Sbjct: 421 GIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNP 480

Query: 481 QGVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           QG+P+GIGFGGLKGFEPRLIEIAYGFEHL   R+ P L R
Sbjct: 481 QGLPFGIGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of MC02g0866 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 836 bits (2159), Expect = 2.61e-302
Identity = 434/522 (83.14%), Postives = 470/522 (90.04%), Query Frame = 0

Query: 1   VAAPSFSLYTSLLLGLQAILS---SGSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVK 60
           +A  SF +Y S+LLGL AILS   SGSCSF+T+ SIEEAT+ D + AFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKD 120
           FYLEQVRR NPILKG+IE+NPDAL+QAS+AD +RKR++  SLSP HGIPVL+KDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQ 180
           KLNTTAGSFALLGS+VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY PPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKLCSFSNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240
           GK      NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV
Sbjct: 181 GK------NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240

Query: 241 GLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQF 300
           GLTSRAGVVPISLRQDTVGPICRTV+DAAYVLDAI GAD YD ST EASKYIP+GGYGQF
Sbjct: 241 GLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQF 300

Query: 301 LRADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGS 360
           LRA+GL+GKRIGIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+   VITGS
Sbjct: 301 LRAEGLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS 360

Query: 361 SSGEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEA 420
           SSGEWTALL EFKISLNAYLK+LV SPI SLS AIEFNK+NSKLEK++EYGQ+LFL+AEA
Sbjct: 361 SSGEWTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEA 420

Query: 421 TKGIRVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGI  AEKAALARL KLSK GFER+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           NPQG+P+GIGFGGLKGF+PRLIEIAYGFEHL   R+ P L R
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of MC02g0866 vs. NCBI nr
Match: ADN34007.1 (amidase [Cucumis melo subsp. melo])

HSP 1 Score: 826 bits (2134), Expect = 1.11e-298
Identity = 429/511 (83.95%), Postives = 463/511 (90.61%), Query Frame = 0

Query: 12  LLLGLQAILS---SGSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVKFYLEQVRRFNP 71
           +LLGL AILS   SGSCSF+T+ SIEEAT+ D + AFYQNKLTS+QLV+FYLEQVRR NP
Sbjct: 1   MLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNP 60

Query: 72  ILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKLNTTAGSFAL 131
           ILKG+IE+NPDAL+QAS+AD +RKR++  SLSP HGIPVL+KDNIATKDKLNTTAGSFAL
Sbjct: 61  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 120

Query: 132 LGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGKLCSFSNNPY 191
           LGS+VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY PPNGWSARGGQGK      NPY
Sbjct: 121 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGK------NPY 180

Query: 192 TLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPI 251
           T+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPI
Sbjct: 181 TMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPI 240

Query: 252 SLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFLRADGLQGKRI 311
           SLRQDTVGPICRTV+DAAYVLDAI GAD YD ST EASKYIP+GGYGQFLRA+GL+GKRI
Sbjct: 241 SLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRI 300

Query: 312 GIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGSSSGEWTALLVE 371
           GIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+   VITGSSSGEWTALL E
Sbjct: 301 GIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAE 360

Query: 372 FKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATKGIRVAEKAA 431
           FKISLNAYLK+LV SPI SLS AIEFNK+NSKLEK++EYGQ+LFL+AEATKGI  AEKAA
Sbjct: 361 FKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAA 420

Query: 432 LARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPYGIGF 491
           LARL KLSK GFER+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P+GIGF
Sbjct: 421 LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGF 480

Query: 492 GGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           GGLKGF+PRLIEIAYGFEHL   R+ P L R
Sbjct: 481 GGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of MC02g0866 vs. NCBI nr
Match: XP_008457662.1 (PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo])

HSP 1 Score: 820 bits (2118), Expect = 3.93e-296
Identity = 430/522 (82.38%), Postives = 466/522 (89.27%), Query Frame = 0

Query: 1   VAAPSFSLYTSLLLGLQAILS---SGSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVK 60
           +A  SF +Y S+LLGL AILS   SGSCSF+T+ SIEEAT+ D + AFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKD 120
           FYLEQVRR NPILKG+IE+NPDAL+QAS+AD +RKR++  SLSP HGIPVL+KDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQ 180
           KLNTTAGSFALLGS+VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY PPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKLCSFSNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240
           GK      NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV
Sbjct: 181 GK------NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240

Query: 241 GLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQF 300
           GLTSRAGVVPISLRQDTVG    TV+DAAYVLDAI GAD YD ST EASKYIP+GGYGQF
Sbjct: 241 GLTSRAGVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQF 300

Query: 301 LRADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGS 360
           LRA+GL+GKRIGIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+   VITGS
Sbjct: 301 LRAEGLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS 360

Query: 361 SSGEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEA 420
           SSGEWTALL EFKISLNAYLK+LV SPI SLS AIEFNK+NSKLEK++EYGQ+LFL+AEA
Sbjct: 361 SSGEWTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEA 420

Query: 421 TKGIRVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGI  AEKAALARL KLSK GFER+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           NPQG+P+GIGFGGLKGF+PRLIEIAYGFEHL   R+ P L R
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 512

BLAST of MC02g0866 vs. NCBI nr
Match: XP_008457661.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis melo subsp. melo] >KAA0045711.1 putative amidase [Cucumis melo var. makuwa] >TYJ99569.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 816 bits (2108), Expect = 1.36e-294
Identity = 430/516 (83.33%), Postives = 460/516 (89.15%), Query Frame = 0

Query: 1   VAAPSFSLYTSLLLGLQAILSS-GSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVKFY 60
           +A  SF LY S+LLGL AILSS GSCSF T  SIEEAT+ DL+HAFYQNKLTS+QLV+FY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  LEQVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKL 120
           LEQVRRFNPILKG+IE+NPDAL QASRAD ERK+++P SLSP HGIPVL+KDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLR AGAIIFGKASLSEWS FRS   P+GWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 LCSFSNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 240
                 NPY LGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL
Sbjct: 181 ------NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 240

Query: 241 TSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFLR 300
           TSRAGV+PIS RQD+VGPICRTVSDA YVLDAIVGAD YD ST EASKYIPKGGYGQFLR
Sbjct: 241 TSRAGVIPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLR 300

Query: 301 ADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGSSS 360
           A GL+GKRIGIVR+FYDFGHD+ FYPQA+EKVLKTLK+GGAILV+N TID+L +I   SS
Sbjct: 301 AGGLKGKRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSS 360

Query: 361 GEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATK 420
           GE  ALL EFKISLNAYLKELV SPI SLS AIEFNK+NSKLE + EYGQ+LFLKAEAT 
Sbjct: 361 GEQIALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATN 420

Query: 421 GIRVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNP 480
           GI  AEKAALARL KLSK GFER+MIKNKLDAIAAPG LIS V AIGGFPGVSVPAGY+P
Sbjct: 421 GIGDAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDP 480

Query: 481 QGVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPP 515
           QGVPYGI FGGLKGFEPRLIEIAYGFEHL KSR+PP
Sbjct: 481 QGVPYGITFGGLKGFEPRLIEIAYGFEHLTKSRKPP 509

BLAST of MC02g0866 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 838 bits (2164), Expect = 2.03e-303
Identity = 433/520 (83.27%), Postives = 469/520 (90.19%), Query Frame = 0

Query: 1   VAAPSFSLYTSLLLGLQAILSS-GSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVKFY 60
           +AA SF +Y S+LLGL AILSS GSCSF+T+ SIEEAT+ DL+ AFYQNKLTS QLV+FY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LEQVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKL 120
           LEQVRRFNPIL G+IE+NPDAL+QAS+AD ERKR++P SLSP HGIPV +KDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVFVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGK 180
           NTTAGSFALLGS+VPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSY  PNGWSARGGQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 LCSFSNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 240
                 NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL
Sbjct: 181 ------NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGL 240

Query: 241 TSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFLR 300
           TSRAGVVPISLRQDTVGPICRTV+DAAYVLDAI G D YD ST EASKY+PKGGYGQFL+
Sbjct: 241 TSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLK 300

Query: 301 ADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGSSS 360
            DGL+GKRIGIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+S HVITGSSS
Sbjct: 301 EDGLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSS 360

Query: 361 GEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATK 420
           GEWTA+L EFKIS+N YLK+LV SPI SLS AIEFN++NSKLEK+KEYGQ+LFL+AEATK
Sbjct: 361 GEWTAVLAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATK 420

Query: 421 GIRVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNP 480
           GI  AEKAALARL KLSK GFER+MIKNKLDAIAAPGRLIS  LAIGGFPGVSVPAGYNP
Sbjct: 421 GIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNP 480

Query: 481 QGVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           QG+P+GIGFGGLKGFEPRLIEIAYGFEHL   R+ P L R
Sbjct: 481 QGLPFGIGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of MC02g0866 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 836 bits (2159), Expect = 1.27e-302
Identity = 434/522 (83.14%), Postives = 470/522 (90.04%), Query Frame = 0

Query: 1   VAAPSFSLYTSLLLGLQAILS---SGSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVK 60
           +A  SF +Y S+LLGL AILS   SGSCSF+T+ SIEEAT+ D + AFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKD 120
           FYLEQVRR NPILKG+IE+NPDAL+QAS+AD +RKR++  SLSP HGIPVL+KDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQ 180
           KLNTTAGSFALLGS+VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY PPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKLCSFSNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240
           GK      NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV
Sbjct: 181 GK------NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240

Query: 241 GLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQF 300
           GLTSRAGVVPISLRQDTVGPICRTV+DAAYVLDAI GAD YD ST EASKYIP+GGYGQF
Sbjct: 241 GLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQF 300

Query: 301 LRADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGS 360
           LRA+GL+GKRIGIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+   VITGS
Sbjct: 301 LRAEGLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS 360

Query: 361 SSGEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEA 420
           SSGEWTALL EFKISLNAYLK+LV SPI SLS AIEFNK+NSKLEK++EYGQ+LFL+AEA
Sbjct: 361 SSGEWTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEA 420

Query: 421 TKGIRVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGI  AEKAALARL KLSK GFER+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           NPQG+P+GIGFGGLKGF+PRLIEIAYGFEHL   R+ P L R
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of MC02g0866 vs. ExPASy TrEMBL
Match: A0A1S3C7A8 (putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 836 bits (2159), Expect = 1.27e-302
Identity = 434/522 (83.14%), Postives = 470/522 (90.04%), Query Frame = 0

Query: 1   VAAPSFSLYTSLLLGLQAILS---SGSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVK 60
           +A  SF +Y S+LLGL AILS   SGSCSF+T+ SIEEAT+ D + AFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKD 120
           FYLEQVRR NPILKG+IE+NPDAL+QAS+AD +RKR++  SLSP HGIPVL+KDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQ 180
           KLNTTAGSFALLGS+VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY PPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKLCSFSNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240
           GK      NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV
Sbjct: 181 GK------NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240

Query: 241 GLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQF 300
           GLTSRAGVVPISLRQDTVGPICRTV+DAAYVLDAI GAD YD ST EASKYIP+GGYGQF
Sbjct: 241 GLTSRAGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQF 300

Query: 301 LRADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGS 360
           LRA+GL+GKRIGIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+   VITGS
Sbjct: 301 LRAEGLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS 360

Query: 361 SSGEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEA 420
           SSGEWTALL EFKISLNAYLK+LV SPI SLS AIEFNK+NSKLEK++EYGQ+LFL+AEA
Sbjct: 361 SSGEWTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEA 420

Query: 421 TKGIRVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGI  AEKAALARL KLSK GFER+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           NPQG+P+GIGFGGLKGF+PRLIEIAYGFEHL   R+ P L R
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of MC02g0866 vs. ExPASy TrEMBL
Match: E5GC08 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 826 bits (2134), Expect = 5.37e-299
Identity = 429/511 (83.95%), Postives = 463/511 (90.61%), Query Frame = 0

Query: 12  LLLGLQAILS---SGSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVKFYLEQVRRFNP 71
           +LLGL AILS   SGSCSF+T+ SIEEAT+ D + AFYQNKLTS+QLV+FYLEQVRR NP
Sbjct: 1   MLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNP 60

Query: 72  ILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKLNTTAGSFAL 131
           ILKG+IE+NPDAL+QAS+AD +RKR++  SLSP HGIPVL+KDNIATKDKLNTTAGSFAL
Sbjct: 61  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 120

Query: 132 LGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGKLCSFSNNPY 191
           LGS+VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY PPNGWSARGGQGK      NPY
Sbjct: 121 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGK------NPY 180

Query: 192 TLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPI 251
           T+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPI
Sbjct: 181 TMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPI 240

Query: 252 SLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFLRADGLQGKRI 311
           SLRQDTVGPICRTV+DAAYVLDAI GAD YD ST EASKYIP+GGYGQFLRA+GL+GKRI
Sbjct: 241 SLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRI 300

Query: 312 GIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGSSSGEWTALLVE 371
           GIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+   VITGSSSGEWTALL E
Sbjct: 301 GIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAE 360

Query: 372 FKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATKGIRVAEKAA 431
           FKISLNAYLK+LV SPI SLS AIEFNK+NSKLEK++EYGQ+LFL+AEATKGI  AEKAA
Sbjct: 361 FKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAA 420

Query: 432 LARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPYGIGF 491
           LARL KLSK GFER+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P+GIGF
Sbjct: 421 LARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGF 480

Query: 492 GGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           GGLKGF+PRLIEIAYGFEHL   R+ P L R
Sbjct: 481 GGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of MC02g0866 vs. ExPASy TrEMBL
Match: A0A1S3C652 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 820 bits (2118), Expect = 1.90e-296
Identity = 430/522 (82.38%), Postives = 466/522 (89.27%), Query Frame = 0

Query: 1   VAAPSFSLYTSLLLGLQAILS---SGSCSFETDPSIEEATVNDLRHAFYQNKLTSKQLVK 60
           +A  SF +Y S+LLGL AILS   SGSCSF+T+ SIEEAT+ D + AFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLEQVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKD 120
           FYLEQVRR NPILKG+IE+NPDAL+QAS+AD +RKR++  SLSP HGIPVL+KDNIATKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQ 180
           KLNTTAGSFALLGS+VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY PPNGWSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKLCSFSNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240
           GK      NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV
Sbjct: 181 GK------NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTV 240

Query: 241 GLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQF 300
           GLTSRAGVVPISLRQDTVG    TV+DAAYVLDAI GAD YD ST EASKYIP+GGYGQF
Sbjct: 241 GLTSRAGVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQF 300

Query: 301 LRADGLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVITGS 360
           LRA+GL+GKRIGIVRK YDFGHDD FY  AFEKV KTLKQGGAILV+NLTI+   VITGS
Sbjct: 301 LRAEGLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS 360

Query: 361 SSGEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEA 420
           SSGEWTALL EFKISLNAYLK+LV SPI SLS AIEFNK+NSKLEK++EYGQ+LFL+AEA
Sbjct: 361 SSGEWTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEA 420

Query: 421 TKGIRVAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGY 480
           TKGI  AEKAALARL KLSK GFER+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGY
Sbjct: 421 TKGIGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGY 480

Query: 481 NPQGVPYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPLKR 519
           NPQG+P+GIGFGGLKGF+PRLIEIAYGFEHL   R+ P L R
Sbjct: 481 NPQGLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 512

BLAST of MC02g0866 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 471.1 bits (1211), Expect = 1.2e-132
Identity = 269/513 (52.44%), Postives = 342/513 (66.67%), Query Frame = 0

Query: 7   SLYTSLLLGLQAILSSGSCS---FETDPSIEEATVNDLRHAFYQNKLTSKQLVKFYLEQV 66
           SL   L+L    I+S GS S     +  SI+EAT+ D+R AF + +LTSKQLV+ YLE +
Sbjct: 11  SLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAI 70

Query: 67  RRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKLNTTA 126
            + NPIL  VIE NPDAL QA  AD ER       L   HG+PVLLKD+I+TKDKLNTTA
Sbjct: 71  SKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTA 130

Query: 127 GSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGKLCSF 186
           GSFALLGSVV RDAGVV +LR++GA+I GKASLSEW+ FRS++ P+GWSA          
Sbjct: 131 GSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA---------- 190

Query: 187 SNNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 246
                                                   PS  NSVVGIKP+VGLTSRA
Sbjct: 191 ----------------------------------------PSQ-NSVVGIKPSVGLTSRA 250

Query: 247 GVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFLRADGL 306
           GVVPISLRQD++GPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL   GL
Sbjct: 251 GVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGL 310

Query: 307 QGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSLHVIT-GSSSGEW 366
           +GKR+GIV K          +    +  +KTL++ GAI++ NLTI ++ VI  G+ SGE 
Sbjct: 311 KGKRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEE 370

Query: 367 TALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATKGIR 426
            ALL EFK+SLNAYLKELV SP+ SL+  I +N+  ++ EK+KE+GQ++FL AEAT G+ 
Sbjct: 371 IALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMG 430

Query: 427 VAEKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGV 486
             EK AL ++++LS+ G E+++ +NKLDAI   G  +S VLAIGG+PG++VPAGY+  GV
Sbjct: 431 EKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGV 462

Query: 487 PYGIGFGGLKGFEPRLIEIAYGFEHLAKSRRPP 516
           PYGI FGGL+  EP+LIEIA+ FE     R+PP
Sbjct: 491 PYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of MC02g0866 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.6e-28
Identity = 147/545 (26.97%), Postives = 239/545 (43.85%), Query Frame = 0

Query: 5   SFSLYTSLLLGLQAILSSGSCSFETDPSI---EEATVNDLRHAFYQNKLTSKQLVKFYLE 64
           S SL       +   + S + S   D S+    ++ +   R +    + T+ ++ K YL 
Sbjct: 10  SLSLLPLRRFQISKTIVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLS 69

Query: 65  QVRRFNPILKGVIELNPDALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKLNT 124
           ++R   P LK  + ++ + L  A   D    +     L P  G+ + +KDNI T+  + +
Sbjct: 70  RIRLTEPQLKCFLHVSENVLKDAQEIDQRIAKG--EELGPLAGVLIGVKDNICTQG-MPS 129

Query: 125 TAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGKLC 184
           TA S  L     P DA  V K+++ G I+ GK ++ E+               G   +  
Sbjct: 130 TAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEF-------------GMGSTTEAS 189

Query: 185 SF--SNNPYTLGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVG 244
           +F  + NP+ L   P GSS GSA +VAA    VSLG++T GS+  P++   VVG+KPT G
Sbjct: 190 AFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYG 249

Query: 245 LTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVGADSYDISTTEASKYIPKGGYGQFL 304
             SR G++  +   D +G    TV+DA  +L AI G D +D  +T + + +P+    QFL
Sbjct: 250 RVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFD--STSSKQDVPE-FQSQFL 309

Query: 305 RAD-----GLQGKRIGIVRKFYDFGHDDAFYPQAFEKVLKTLKQGGAILVE------NLT 364
             D      L G ++GI+R+  + G D      A ++    L+  G IL E      +L 
Sbjct: 310 SVDHFESKPLNGVKVGIIRETLEDGVDSG-VRSATQEAASHLEALGCILTEVSLPSFSLG 369

Query: 365 IDSLHVITGSSSGEWTALLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEY 424
           + + +VI  S S    +     +       +EL  + +   S    F         +  Y
Sbjct: 370 LPAYYVIASSESSSNLSRYDGVRYGNQVMAEEL--NKLYECSRGEGFGGEVKMRILMGTY 429

Query: 425 G-----QDLFLK--AEATKGIRVAEKAALARLEKLSKGGFERVMIK---NKLDAIAA-PG 484
                  D + K   +    IR   KAAL + + L          K    K D +A   G
Sbjct: 430 ALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAG 489

Query: 485 RLISPVLAIGGFPGVSVPAGY---NPQGVPYGIGFGGLKGFEPRLIEIAYGFEHLAK-SR 518
            +++  + + G P + +P G     P G+P G+   G    E +L+++ + FE   K S 
Sbjct: 490 DIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKGSS 532

BLAST of MC02g0866 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 120.2 bits (300), Expect = 5.0e-27
Identity = 87/250 (34.80%), Postives = 132/250 (52.80%), Query Frame = 0

Query: 37  TVNDLRHAFYQNKLTSKQLVKFYLEQVRRFNPILKGVIELNPD-ALHQASRADYERKRNA 96
           +V +L       ++TSK+LV+ YL+Q++R+N +L+ V+    + A  QA  AD    +  
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQG- 263

Query: 97  PASLSPSHGIPVLLKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKAS 156
              L P HGIP  LKD +A      TT GS +     +  +A V  +L+ +GA++  K  
Sbjct: 264 -TYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLV 323

Query: 157 LSEWSDFRSYAPPNGWSARGGQGKLCSFSNNPYTLGE-PCGSSSGSAISVAANMVTVSLG 216
                   S A  + W   GG+      + NP+ + E   GSS+G A S +A MV  ++G
Sbjct: 324 TG------SMAYDDIWF--GGR------TRNPWNIEEFSTGSSAGPAASTSAGMVPFAIG 383

Query: 217 TETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVG 276
           +ET GS+  P+    +  ++PT G   R GV+ IS   D +GP CRT +D A +LDAI G
Sbjct: 384 SETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKG 436

Query: 277 ADSYDISTTE 285
            D  D+S+ E
Sbjct: 444 KDPDDLSSRE 436

BLAST of MC02g0866 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 109.8 bits (273), Expect = 6.8e-24
Identity = 85/250 (34.00%), Postives = 128/250 (51.20%), Query Frame = 0

Query: 37  TVNDLRHAFYQNKLTSKQLVKFYLEQVRRFNPILKGVIELNPD-ALHQASRADYERKRNA 96
           +V +L       ++TSK+LV+ YL+Q++R+N +L+ V+    + A  QA  AD    +  
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQG- 263

Query: 97  PASLSPSHGIPVLLKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLRKAGAIIFGKAS 156
              L P HGIP  LKD +A      TT GS +     +  +A V  +L+ +GA++  K  
Sbjct: 264 -TYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLV 323

Query: 157 LSEWSDFRSYAPPNGWSARGGQGKLCSFSNNPYTLGE-PCGSSSGSAISVAANMVTVSLG 216
                   S A  + W   GG+      + NP+ + E   GSS+G A S +A       G
Sbjct: 324 TG------SMAYDDIWF--GGR------TRNPWNIEEFSTGSSAGPAASTSA-------G 383

Query: 217 TETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPICRTVSDAAYVLDAIVG 276
           +ET GS+  P+    +  ++PT G   R GV+ IS   D +GP CRT +D A +LDAI G
Sbjct: 384 SETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKG 429

Query: 277 ADSYDISTTE 285
            D  D+S+ E
Sbjct: 444 KDPDDLSSRE 429

BLAST of MC02g0866 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 95.9 bits (237), Expect = 1.0e-19
Identity = 127/513 (24.76%), Postives = 202/513 (39.38%), Query Frame = 0

Query: 24  SCSFETDP--SIEEATVNDLRHAFYQNKLTSKQLVKFYLEQVRRF---NPILKGVIELNP 83
           S S   DP  S     + D  +A+     T  Q+ K  +  +  F    P    +I  + 
Sbjct: 115 SRSLHADPVSSFRYWKIRDYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFDA 174

Query: 84  DALHQASRADYERKRNAPASLSPSHGIPVLLKDNIATKDKLNTTAGSFALLGSVVPRDAG 143
           + + + + A   R+      +S   GI V +KD+I           ++      V +D+ 
Sbjct: 175 NEVIKQAEAS-TRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSA 234

Query: 144 VVTKLRKAGAIIFGKASLSEWSDFRSYAPPNGWSARGGQGKLCSFSNNPYTLGEPCGSSS 203
           VV+KLR  GAI+ GKA++ E           G    G      +  N         GSSS
Sbjct: 235 VVSKLRSCGAILLGKANMHEL----------GMGTTGNNSNYGTTRNPHDPKRYTGGSSS 294

Query: 204 GSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPI 263
           GSA  VAA + + +LGT+  GS+  PS L  + G+K T G T   G +      + +GP+
Sbjct: 295 GSAAIVAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPL 354

Query: 264 CRTVSDAAYVLDAIVGADSYDISTTEAS-----KYIPKGGYGQFLRADGLQGKRIGIVRK 323
             ++ DA  V  AI+G+ S D    + S     K +   G      ++ +   R+G   K
Sbjct: 355 ASSLEDAFLVYAAILGSSSADRYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTK 414

Query: 324 -FYDFGHDDAFYPQAFEKVLKTLKQGGAILVENLTIDSL------HVIT-GSSS-GEWTA 383
            F D    D       E +LK L       V  + +  L      HVI+ GS +    T 
Sbjct: 415 WFNDVSSSD--ISDKCEDILKLLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTP 474

Query: 384 LLVEFKISLNAYLKELVVSPIXSLSYAIEFNKRNSKLEKIKEYGQDLFLKAEATKGIRVA 443
                K S  +Y      +   S S A ++        ++ EY  ++F   +        
Sbjct: 475 YCEAGKNSKLSYDTRTSFAIFRSFS-ASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTG 534

Query: 444 EKAALARLEKLSKGGFERVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPY 503
             A +   + L K G   + +   L        L+       GFP +SVP GY+ +G+P 
Sbjct: 535 MTAPVIPPDAL-KNGETNIQVTTDLMRFVLAANLL-------GFPAISVPVGYDKEGLPI 594

Query: 504 GIGFGGLKGFEPRLIEIAYGFEHLAKSRRPPPL 518
           G+   G    E  ++ +A   E LA   + P +
Sbjct: 595 GLQIMGRPWAEATVLGLAAAVEELAPVTKKPAI 599