MC02g0845 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0845
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein kinase domain-containing protein
LocationMC02: 6691668 .. 6694755 (-)
RNA-Seq ExpressionMC02g0845
SyntenyMC02g0845
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGTTGGTCAGGAGGATTCTGAGGATATTGAGAAGAAGAAGAAGAAAAAGAAGAAGAAGAAGAATGTGTTAGTGGGGATTCGGATTAATGGTGATGGTAGAGACCTGCTCAATTGGGCGATTGTTAAAGTTGCTGACCCTGGGGATTGTGTCATTGTAATCCATGTTTGTCAAAATTCTGGTGGGGGGTTTTTTTTTTCAAACCCACCCCAATTTCTGGTTTTAATTCTTTTACATCTTTACTTTTTTGCATAGTTTTGAAATATGTCTTTTCCCTCTTGCAAAACAGACCGTGCACTAAAAACTAAGCCCTTATTTGATGAATTCTTAGAAGGATACAAAAGCCTTTGTAATGTAAACAAGGTTCGGTTTGGAATTTGTCGTCCTCTATTGATATTTATGGATGGAATGAAGTTTTGGGTACTGATTATTGTCTTCAAATTCGGGTATTAGGTAGCTTTCATTTCTCATATATCGACCGGAAGTTCGGTTAAGACGACTTTGGTCAGAGAGGCAGAATGCTATGATGCTCGGGCTGTAATTTTGGGAACGAGCAAGTTGTCTACTAATCTTGGGTAGGGAAGTCATAGAAAACTCGAGTCGTTTTTTCGTTTTGGTTGGTCGTAGGGGTTTTTTTTTTGTTTGTTTGTTTTCTGATTTGGTCTTAACAGAAACCATTTGCAGGGGTTGGTCAACAACAACTAGATACTTTGTTAAGCGACTGCCTCCAACCACTGATGTTTTGGCTCTCTACAAAGGCAAAGTTGTCTTCAGAAGGTCCATCAGTGATCAACTTCCAGGTTTGGTGTTCTTGGCCTAAAATACAATTCATTGGTAGATTGAATTTTTTTCTTCTTTTTTAAAGCAAAATTGGTCATAGTTTTGACTGTAATATGACAGGCTTGAGTCAAGACCCAAAACCAAGTTTTAGTCCAGCTACTCAATCTGACTTTGATGGTTCTGAGACCAAGAAATCTTTTTCTTATGGGGTTGGTAGTGGAGACATGAAGGATGTTCATGGAGTTGTCCTTAAAAGTAAAAGAAATTGTTCCAAACAGGACACTCCAATGAAGACAGACAATTCCGAGGGGGGTCTTGGTTGGCCGTTGCTCCGTACATCTCCGAAGATTTCGCAAACCCTGTCTGTACATGACATGTCTGTGGTGCAATGGGTGATGAACTTACCAGATCGTTCGCCATCTCACAGTTTAAGAATAAAGGGAAATGATCCATCAAGAAGAGAGATCTCTGATTTTCTGGATGAGAGGAATAAAGGCAGTTTATCTGCCTTTTCTGAGCGACCACAAAAATTGAAGGATCTCCTGAAAGCTAGCTCATCTTGTTATAAGTGGTTTAGTCCTGATGTGCTGAAAACTTCAACTTCTCATTTCTCTTCAGGTTTTCGAGTCAATACTTGCTTTGAATTGATTGTTACGTTCACTTTCTCGCCATTCAGATTAAGTATGTTCTTGGTCTGTCCATGACCCATAAATTTATTCTTTCTTAATAGAAAACTTGATTGGGAAAGGTGGTTGCAATCGTGTATACAAAGGAATCCTCCCAGATGGAAAGCCAATCGCGGTTAAGGTAATGAACTCATCCGAAAAGGCATGGAACGAATTTTCTCGGGAAGTTGACATTATATCCTCATTGCATCACAAAAATATCACTCCCTTTCTTGGTGTTTGCATCAAAGACGATACATTGATCTCTGTTTATGATTTCTTTTCCCAAGGAAGCTTAGAGGAAAATCTATACGGTAAGGCTTAAATATTAGAATTCTTTCTTCTACAATGTGAAAAACTATCAGATCAGGACCACGCTGATAAGAACATGATATGCAATTTAGGTAGAAACAAAGAAAAAGGCATACTTTCCTGGGTGGTAAGATTCAAGTTGGCTGTTGGAATTGCTGAGGCACTAAACTACCTGCACGAGGAATCTCCCCGGCCTGTTGTACACAGAGATGTCAAGACTTCAAACATTCTTCTCACTGATGAACTTGAACCACAGGTAGTTCGTTATAACATCCTCATCGGTTGTGATTTTGAATATTTCTCGTTTATTCCTAACTGAGGTTCTATATAACAGTTGTCTGATTTTGGCCTTGCAATCTGGGGACCAACCGAATCATCCTTTCAGATCGAAGCCGATGTTGTGGGAACGTTTGGATATCTTGCTCCAGAGTATTTCATGTACGGAAAAATGAGTGATAAGGTTGATGTCTACGCCTTTGGCATAGTTCTACTTGAATTATTATCGGGACGAAGAGCAATCGACACTGAGACTTCGAAAGAGCAACAGAGCTTGGTTATGTGGGTATGACTTCCATCAAGTGTACTACTTATACTTCAAATGATTTAAGATGCTCTATGATTGTACAAGGATGAAGACCACTTATCATTTCATGTCAGGCAAAGCCAATCATAGAACGCGGTGACGTAAAGGGCATAGTCGATCCAAACTTGGAGGGAAAATTCGACAATGTTCAGTTGAAGAGAATGGTTCTTGCAGCAACCCTGTGCATCACGAGGGCATCTCGACTCCGTCCTAGAATTACTCAGGTCTTGTATTCAAGCGATACTCACCAACATATCACTGACATCACACATTACTGACATCGCACATTACTTGTATTAATCAAAGATGTTCACAAAAATGCCTTTCGCCCCAGATAATGAAGGTTCTAAGAGGGGAAATTGATACTGAAAGCATCGTTGTGGAGGATTCACAAAGTGTGGAGAATGGAGATGACGAAGTTTACCCGAGTTCGAGTTCTGAACTACACTTGAGCCTTGCATTGCTAGGTGTTGATGATGGTGGTGGGGATTCATTTAGCAGTGTGGAACAGAAAAAAAGAGTTTGTTTAGAAGATTATTTGAAAGAAAGATGGAGCAGGTCCTCAAGCTTCAACTATCTTCCTTAGAGATATGTCAGTTTCCCTTCAGCTTAGACTGTAAATTAGCCAAACATTAAACAATGTCATCAAAATTTAAACTGTACAATGACTATAAACTTATGCACCCTTTGGTGAGGTGATTTCTGTAAATATATATATATTATACATCTTTTTTTTGGGTCCACC

mRNA sequence

ATGACTGTTGGTCAGGAGGATTCTGAGGATATTGAGAAGAAGAAGAAGAAAAAGAAGAAGAAGAAGAATGTGTTAGTGGGGATTCGGATTAATGGTGATGGTAGAGACCTGCTCAATTGGGCGATTGTTAAAGTTGCTGACCCTGGGGATTGTGTCATTGTAATCCATGTTTGTCAAAATTCTGACCGTGCACTAAAAACTAAGCCCTTATTTGATGAATTCTTAGAAGGATACAAAAGCCTTTGTAATGTAAACAAGGTAGCTTTCATTTCTCATATATCGACCGGAAGTTCGGTTAAGACGACTTTGGTCAGAGAGGCAGAATGCTATGATGCTCGGGCTGTAATTTTGGGAACGAGCAAGTTGTCTACTAATCTTGGGGGTTGGTCAACAACAACTAGATACTTTGTTAAGCGACTGCCTCCAACCACTGATGTTTTGGCTCTCTACAAAGGCAAAGTTGTCTTCAGAAGGTCCATCAGTGATCAACTTCCAGGCTTGAGTCAAGACCCAAAACCAAGTTTTAGTCCAGCTACTCAATCTGACTTTGATGGTTCTGAGACCAAGAAATCTTTTTCTTATGGGGTTGGTAGTGGAGACATGAAGGATGTTCATGGAGTTGTCCTTAAAAGTAAAAGAAATTGTTCCAAACAGGACACTCCAATGAAGACAGACAATTCCGAGGGGGGTCTTGGTTGGCCGTTGCTCCGTACATCTCCGAAGATTTCGCAAACCCTGTCTGTACATGACATGTCTGTGGTGCAATGGGTGATGAACTTACCAGATCGTTCGCCATCTCACAGTTTAAGAATAAAGGGAAATGATCCATCAAGAAGAGAGATCTCTGATTTTCTGGATGAGAGGAATAAAGGCAGTTTATCTGCCTTTTCTGAGCGACCACAAAAATTGAAGGATCTCCTGAAAGCTAGCTCATCTTGTTATAAGTGGTTTAGTCCTGATGTGCTGAAAACTTCAACTTCTCATTTCTCTTCAGAAAACTTGATTGGGAAAGGTGGTTGCAATCGTGTATACAAAGGAATCCTCCCAGATGGAAAGCCAATCGCGGTTAAGGTAATGAACTCATCCGAAAAGGCATGGAACGAATTTTCTCGGGAAGTTGACATTATATCCTCATTGCATCACAAAAATATCACTCCCTTTCTTGGTGTTTGCATCAAAGACGATACATTGATCTCTGTTTATGATTTCTTTTCCCAAGGAAGCTTAGAGGAAAATCTATACGGTAGAAACAAAGAAAAAGGCATACTTTCCTGGGTGGTAAGATTCAAGTTGGCTGTTGGAATTGCTGAGGCACTAAACTACCTGCACGAGGAATCTCCCCGGCCTGTTGTACACAGAGATGTCAAGACTTCAAACATTCTTCTCACTGATGAACTTGAACCACAGTTGTCTGATTTTGGCCTTGCAATCTGGGGACCAACCGAATCATCCTTTCAGATCGAAGCCGATGTTGTGGGAACGTTTGGATATCTTGCTCCAGAGTATTTCATGTACGGAAAAATGAGTGATAAGGTTGATGTCTACGCCTTTGGCATAGTTCTACTTGAATTATTATCGGGACGAAGAGCAATCGACACTGAGACTTCGAAAGAGCAACAGAGCTTGGTTATGTGGGCAAAGCCAATCATAGAACGCGGTGACGTAAAGGGCATAGTCGATCCAAACTTGGAGGGAAAATTCGACAATGTTCAGTTGAAGAGAATGGTTCTTGCAGCAACCCTGTGCATCACGAGGGCATCTCGACTCCGTCCTAGAATTACTCAGATAATGAAGGTTCTAAGAGGGGAAATTGATACTGAAAGCATCGTTGTGGAGGATTCACAAAGTGTGGAGAATGGAGATGACGAAGTTTACCCGAGTTCGAGTTCTGAACTACACTTGAGCCTTGCATTGCTAGGTGTTGATGATGGTGGTGGGGATTCATTTAGCAGTGTGGAACAGAAAAAAAGAGTTTGTTTAGAAGATTATTTGAAAGAAAGATGGAGCAGGTCCTCAAGCTTCAACTATCTTCCTTAGAGATATGTCAGTTTCCCTTCAGCTTAGACTGTAAATTAGCCAAACATTAAACAATGTCATCAAAATTTAAACTGTACAATGACTATAAACTTATGCACCCTTTGGTGAGGTGATTTCTGTAAATATATATATATTATACATCTTTTTTTTGGGTCCACC

Coding sequence (CDS)

ATGACTGTTGGTCAGGAGGATTCTGAGGATATTGAGAAGAAGAAGAAGAAAAAGAAGAAGAAGAAGAATGTGTTAGTGGGGATTCGGATTAATGGTGATGGTAGAGACCTGCTCAATTGGGCGATTGTTAAAGTTGCTGACCCTGGGGATTGTGTCATTGTAATCCATGTTTGTCAAAATTCTGACCGTGCACTAAAAACTAAGCCCTTATTTGATGAATTCTTAGAAGGATACAAAAGCCTTTGTAATGTAAACAAGGTAGCTTTCATTTCTCATATATCGACCGGAAGTTCGGTTAAGACGACTTTGGTCAGAGAGGCAGAATGCTATGATGCTCGGGCTGTAATTTTGGGAACGAGCAAGTTGTCTACTAATCTTGGGGGTTGGTCAACAACAACTAGATACTTTGTTAAGCGACTGCCTCCAACCACTGATGTTTTGGCTCTCTACAAAGGCAAAGTTGTCTTCAGAAGGTCCATCAGTGATCAACTTCCAGGCTTGAGTCAAGACCCAAAACCAAGTTTTAGTCCAGCTACTCAATCTGACTTTGATGGTTCTGAGACCAAGAAATCTTTTTCTTATGGGGTTGGTAGTGGAGACATGAAGGATGTTCATGGAGTTGTCCTTAAAAGTAAAAGAAATTGTTCCAAACAGGACACTCCAATGAAGACAGACAATTCCGAGGGGGGTCTTGGTTGGCCGTTGCTCCGTACATCTCCGAAGATTTCGCAAACCCTGTCTGTACATGACATGTCTGTGGTGCAATGGGTGATGAACTTACCAGATCGTTCGCCATCTCACAGTTTAAGAATAAAGGGAAATGATCCATCAAGAAGAGAGATCTCTGATTTTCTGGATGAGAGGAATAAAGGCAGTTTATCTGCCTTTTCTGAGCGACCACAAAAATTGAAGGATCTCCTGAAAGCTAGCTCATCTTGTTATAAGTGGTTTAGTCCTGATGTGCTGAAAACTTCAACTTCTCATTTCTCTTCAGAAAACTTGATTGGGAAAGGTGGTTGCAATCGTGTATACAAAGGAATCCTCCCAGATGGAAAGCCAATCGCGGTTAAGGTAATGAACTCATCCGAAAAGGCATGGAACGAATTTTCTCGGGAAGTTGACATTATATCCTCATTGCATCACAAAAATATCACTCCCTTTCTTGGTGTTTGCATCAAAGACGATACATTGATCTCTGTTTATGATTTCTTTTCCCAAGGAAGCTTAGAGGAAAATCTATACGGTAGAAACAAAGAAAAAGGCATACTTTCCTGGGTGGTAAGATTCAAGTTGGCTGTTGGAATTGCTGAGGCACTAAACTACCTGCACGAGGAATCTCCCCGGCCTGTTGTACACAGAGATGTCAAGACTTCAAACATTCTTCTCACTGATGAACTTGAACCACAGTTGTCTGATTTTGGCCTTGCAATCTGGGGACCAACCGAATCATCCTTTCAGATCGAAGCCGATGTTGTGGGAACGTTTGGATATCTTGCTCCAGAGTATTTCATGTACGGAAAAATGAGTGATAAGGTTGATGTCTACGCCTTTGGCATAGTTCTACTTGAATTATTATCGGGACGAAGAGCAATCGACACTGAGACTTCGAAAGAGCAACAGAGCTTGGTTATGTGGGCAAAGCCAATCATAGAACGCGGTGACGTAAAGGGCATAGTCGATCCAAACTTGGAGGGAAAATTCGACAATGTTCAGTTGAAGAGAATGGTTCTTGCAGCAACCCTGTGCATCACGAGGGCATCTCGACTCCGTCCTAGAATTACTCAGATAATGAAGGTTCTAAGAGGGGAAATTGATACTGAAAGCATCGTTGTGGAGGATTCACAAAGTGTGGAGAATGGAGATGACGAAGTTTACCCGAGTTCGAGTTCTGAACTACACTTGAGCCTTGCATTGCTAGGTGTTGATGATGGTGGTGGGGATTCATTTAGCAGTGTGGAACAGAAAAAAAGAGTTTGTTTAGAAGATTATTTGAAAGAAAGATGGAGCAGGTCCTCAAGCTTCAACTATCTTCCTTAG

Protein sequence

MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQNSDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTSKLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQSDFDGSETKKSFSYGVGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTSPKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSERPQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMNSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEKGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGPTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVLRGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVCLEDYLKERWSRSSSFNYLP
Homology
BLAST of MC02g0845 vs. ExPASy Swiss-Prot
Match: Q9SGY7 (Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thaliana OX=3702 GN=PERK11 PE=2 SV=2)

HSP 1 Score: 249.6 bits (636), Expect = 1.0e-64
Identity = 129/325 (39.69%), Postives = 202/325 (62.15%), Query Frame = 0

Query: 317 FSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMNS-SEKAWNEFSREVDI 376
           F+ + L   T  F    ++G+GG   VYKGIL +GKP+A+K + S S + + EF  EV+I
Sbjct: 358 FTYEELSQITEGFCKSFVVGEGGFGCVYKGILFEGKPVAIKQLKSVSAEGYREFKAEVEI 417

Query: 377 ISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEKGILSWVVRFKLAVGI 436
           IS +HH+++   +G CI +     +Y+F    +L+ +L+G+N    +L W  R ++A+G 
Sbjct: 418 ISRVHHRHLVSLVGYCISEQHRFLIYEFVPNNTLDYHLHGKNLP--VLEWSRRVRIAIGA 477

Query: 437 AEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGPTESSFQIEADVVGTF 496
           A+ L YLHE+    ++HRD+K+SNILL DE E Q++DFGLA    T  S  I   V+GTF
Sbjct: 478 AKGLAYLHEDCHPKIIHRDIKSSNILLDDEFEAQVADFGLARLNDTAQS-HISTRVMGTF 537

Query: 497 GYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQSLVMWAKP----IIE 556
           GYLAPEY   GK++D+ DV++FG+VLLEL++GR+ +DT     ++SLV WA+P     IE
Sbjct: 538 GYLAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDTSQPLGEESLVEWARPRLIEAIE 597

Query: 557 RGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVLRGEIDTESIVV 616
           +GD+  +VDP LE  +   ++ +M+  A  C+  ++  RPR+ Q+++ L    DT   + 
Sbjct: 598 KGDISEVVDPRLENDYVESEVYKMIETAASCVRHSALKRPRMVQVVRAL----DTRDDLS 657

Query: 617 EDSQSVENGDDEVYPSS--SSELHL 635
           + +  V+ G   VY S   S+E+ +
Sbjct: 658 DLTNGVKVGQSRVYDSGQYSNEIRI 675

BLAST of MC02g0845 vs. ExPASy Swiss-Prot
Match: Q8RXC8 (Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thaliana OX=3702 GN=RBK2 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.3e-64
Identity = 132/305 (43.28%), Postives = 198/305 (64.92%), Query Frame = 0

Query: 303 LKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVK--VMN 362
           L D+    SS    FS   ++ +T +FS EN+IG+GG   VY+GILP+GK IAVK     
Sbjct: 118 LHDIYDFQSSLQN-FSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKLIAVKRLTKG 177

Query: 363 SSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEK 422
           + ++   EF  E+ II+ + H N   F+G CI+    + V+     GSL   L+G +K K
Sbjct: 178 TPDEQTAEFLSELGIIAHVDHPNTAKFIGCCIEGGMHL-VFRLSPLGSLGSLLHGPSKYK 237

Query: 423 GILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGP 482
             L+W  R+ +A+G A+ L YLHE   R ++HRD+K  NILLT++ +PQ+ DFGLA W P
Sbjct: 238 --LTWSRRYNVALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICDFGLAKWLP 297

Query: 483 TESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQ 542
            + +    +   GTFGY APEYFM+G + +K DV+AFG++LLEL++G  A+D    + QQ
Sbjct: 298 KQLTHHNVSKFEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALD----ESQQ 357

Query: 543 SLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVL 602
           SLV+WAKP++ER  +K +VDP+L  +++  +L R+   A+LCI ++S LRPR++Q++++L
Sbjct: 358 SLVLWAKPLLERKAIKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQVVELL 414

Query: 603 RGEID 606
            G  D
Sbjct: 418 LGHED 414

BLAST of MC02g0845 vs. ExPASy Swiss-Prot
Match: Q8H1D6 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thaliana OX=3702 GN=RBK1 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 8.6e-64
Identity = 128/320 (40.00%), Postives = 204/320 (63.75%), Query Frame = 0

Query: 317 FSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMNS----SEKAWNEFSRE 376
           F+ + L  +T +F+ EN+IGKGG   VYKG+L +G+ +A+K + S     E+  ++F  E
Sbjct: 141 FTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGETVAIKKLMSHAKEEEERVSDFLSE 200

Query: 377 VDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEKGILSWVVRFKLA 436
           + II+ ++H N     G    D  L  V ++   GSL   L+G  +    L W +R+K+A
Sbjct: 201 LGIIAHVNHPNAARLRGFS-SDRGLHFVLEYAPYGSLASMLFGSEE---CLEWKIRYKVA 260

Query: 437 VGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGPTESSFQIEADVV 496
           +GIA+ L+YLH   PR ++HRD+K SNILL  + E Q+SDFGLA W P      +   + 
Sbjct: 261 LGIADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQISDFGLAKWLPENWPHHVVFPIE 320

Query: 497 GTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQSLVMWAKPIIER 556
           GTFGYLAPEYFM+G + +K+DV+AFG++LLE+++ RRA+DT +   +QS+V WAKP +E+
Sbjct: 321 GTFGYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAVDTAS---RQSIVAWAKPFLEK 380

Query: 557 GDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVLRGE---IDTESI 616
             ++ IVDP L   F+  +++R++L A++C+   + +RP +T+++++LRGE    + +  
Sbjct: 381 NSMEDIVDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMTRLVQLLRGEDGPAELQQK 440

Query: 617 VVEDSQSVENGDDEVYPSSS 630
             E + SV   D + + SSS
Sbjct: 441 AGERTMSVNACDLQDHTSSS 453

BLAST of MC02g0845 vs. ExPASy Swiss-Prot
Match: O65530 (Proline-rich receptor-like protein kinase PERK14 OS=Arabidopsis thaliana OX=3702 GN=PERK14 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.1e-63
Identity = 132/322 (40.99%), Postives = 196/322 (60.87%), Query Frame = 0

Query: 317 FSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMN-SSEKAWNEFSREVDI 376
           FS + L  +T  FS ENL+G+GG   V+KG+L +G  +AVK +   S +   EF  EVD 
Sbjct: 377 FSYEELSKATGGFSEENLLGEGGFGYVHKGVLKNGTEVAVKQLKIGSYQGEREFQAEVDT 436

Query: 377 ISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEKGILSWVVRFKLAVGI 436
           IS +HHK++   +G C+  D  + VY+F  + +LE +L+       +L W +R ++AVG 
Sbjct: 437 ISRVHHKHLVSLVGYCVNGDKRLLVYEFVPKDTLEFHLH--ENRGSVLEWEMRLRIAVGA 496

Query: 437 AEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLA-IWGPTESSF-QIEADVVG 496
           A+ L YLHE+    ++HRD+K +NILL  + E ++SDFGLA  +  T SSF  I   VVG
Sbjct: 497 AKGLAYLHEDCSPTIIHRDIKAANILLDSKFEAKVSDFGLAKFFSDTNSSFTHISTRVVG 556

Query: 497 TFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQSLVMWAKPIIERG 556
           TFGY+APEY   GK++DK DVY+FG+VLLEL++GR +I  + S   QSLV WA+P++ + 
Sbjct: 557 TFGYMAPEYASSGKVTDKSDVYSFGVVLLELITGRPSIFAKDSSTNQSLVDWARPLLTKA 616

Query: 557 DVKG-----IVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVLRGEIDTES 616
            + G     +VD  LE  +D  Q+  M   A  CI +++ LRPR++Q+++ L GE+    
Sbjct: 617 -ISGESFDFLVDSRLEKNYDTTQMANMAACAAACIRQSAWLRPRMSQVVRALEGEVALRK 676

Query: 617 IVVEDSQSVENGDDEVYPSSSS 631
           +        E G+   Y SS +
Sbjct: 677 V-------EETGNSVTYSSSEN 688

BLAST of MC02g0845 vs. ExPASy Swiss-Prot
Match: Q9LV48 (Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 GN=PERK1 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 9.5e-63
Identity = 122/316 (38.61%), Postives = 197/316 (62.34%), Query Frame = 0

Query: 317 FSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMNS-SEKAWNEFSREVDI 376
           F+ + L  +T+ FS  NL+G+GG   V+KGILP GK +AVK + + S +   EF  EV+I
Sbjct: 268 FTYEELSRATNGFSEANLLGQGGFGYVHKGILPSGKEVAVKQLKAGSGQGEREFQAEVEI 327

Query: 377 ISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEKGILSWVVRFKLAVGI 436
           IS +HH+++   +G C+     + VY+F    +LE +L+G  K +  + W  R K+A+G 
Sbjct: 328 ISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLEFHLHG--KGRPTMEWSTRLKIALGS 387

Query: 437 AEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGPTESSFQIEADVVGTF 496
           A+ L+YLHE+    ++HRD+K SNIL+  + E +++DFGLA    ++++  +   V+GTF
Sbjct: 388 AKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVADFGLAKIA-SDTNTHVSTRVMGTF 447

Query: 497 GYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQSLVMWAKPII----E 556
           GYLAPEY   GK+++K DV++FG+VLLEL++GRR +D        SLV WA+P++    E
Sbjct: 448 GYLAPEYAASGKLTEKSDVFSFGVVLLELITGRRPVDANNVYVDDSLVDWARPLLNRASE 507

Query: 557 RGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVLRGEIDTESIVV 616
            GD +G+ D  +  ++D  ++ RMV  A  C+  ++R RPR++QI++ L G +    +  
Sbjct: 508 EGDFEGLADSKMGNEYDREEMARMVACAAACVRHSARRRPRMSQIVRALEGNVSLSDL-- 567

Query: 617 EDSQSVENGDDEVYPS 628
             ++ +  G   VY S
Sbjct: 568 --NEGMRPGHSNVYSS 576

BLAST of MC02g0845 vs. NCBI nr
Match: XP_022146129.1 (probable serine/threonine-protein kinase PBL23 isoform X1 [Momordica charantia] >XP_022146130.1 probable serine/threonine-protein kinase PBL23 isoform X1 [Momordica charantia])

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 678/678 (100.00%), Postives = 678/678 (100.00%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN
Sbjct: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS
Sbjct: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ
Sbjct: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180

Query: 181 SDFDGSETKKSFSYGVGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTSP 240
           SDFDGSETKKSFSYGVGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTSP
Sbjct: 181 SDFDGSETKKSFSYGVGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTSP 240

Query: 241 KISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSERP 300
           KISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSERP
Sbjct: 241 KISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSERP 300

Query: 301 QKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMN 360
           QKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMN
Sbjct: 301 QKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMN 360

Query: 361 SSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEK 420
           SSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEK
Sbjct: 361 SSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEK 420

Query: 421 GILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGP 480
           GILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGP
Sbjct: 421 GILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGP 480

Query: 481 TESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQ 540
           TESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQ
Sbjct: 481 TESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQ 540

Query: 541 SLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVL 600
           SLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVL
Sbjct: 541 SLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVL 600

Query: 601 RGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVCL 660
           RGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVCL
Sbjct: 601 RGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVCL 660

Query: 661 EDYLKERWSRSSSFNYLP 678
           EDYLKERWSRSSSFNYLP
Sbjct: 661 EDYLKERWSRSSSFNYLP 678

BLAST of MC02g0845 vs. NCBI nr
Match: XP_008457694.1 (PREDICTED: serine/threonine-protein kinase At3g07070 [Cucumis melo] >XP_008457695.1 PREDICTED: serine/threonine-protein kinase At3g07070 [Cucumis melo])

HSP 1 Score: 1083 bits (2800), Expect = 0.0
Identity = 547/676 (80.92%), Postives = 608/676 (89.94%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTV QEDSE IE++KK KK +KNVLVGIR+NGD RDLLNW+IVKVADPGDCVIVI+VCQ+
Sbjct: 1   MTVDQEDSEAIEEEKKTKKTRKNVLVGIRMNGDSRDLLNWSIVKVADPGDCVIVIYVCQS 60

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRA K KPLFDEFLEGY++LC+VNKVAFI+ I TGSSVK TLVR+A+ Y A AV+LGTS
Sbjct: 61  SDRASKDKPLFDEFLEGYRNLCDVNKVAFIAQIVTGSSVKKTLVRQAKIYAAGAVVLGTS 120

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           K   NLGGWS+ TRYFVKRLPPTTDVLAL  GK+VFRRS +DQL GLS DPKPSFS A+Q
Sbjct: 121 K-PCNLGGWSSITRYFVKRLPPTTDVLALNNGKIVFRRSTNDQLTGLSLDPKPSFSQASQ 180

Query: 181 SDFDGSETKKSFSYGVGSGDMKD-VHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTS 240
           SDFDGSET+KS SYGVGS D+KD V GVVL+SKRNCS+ D+PM  ++SE GLGWPLLRT+
Sbjct: 181 SDFDGSETEKSVSYGVGSEDLKDEVDGVVLESKRNCSEPDSPMMMEHSEPGLGWPLLRTT 240

Query: 241 PKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSER 300
           P+ISQT SVH+MSVVQWVMNLPDRSP  SL IKGNDP R EI  F+DER KGSLS+FSE 
Sbjct: 241 PRISQTSSVHNMSVVQWVMNLPDRSPHRSLSIKGNDPLRSEIPVFVDERAKGSLSSFSEP 300

Query: 301 PQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360
           P+ LKDLLK +S+ YKWFSP VLKTSTSHFSSENLIGKGGCNRVYKGILP+GKP+AVKVM
Sbjct: 301 PEDLKDLLKTNSTTYKWFSPYVLKTSTSHFSSENLIGKGGCNRVYKGILPNGKPVAVKVM 360

Query: 361 NSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKE 420
           NSS++AWNEFSREVDI+SSL HKNITPFLG+CI D+ LISVYDFFS+GSLE NLYGRNKE
Sbjct: 361 NSSKQAWNEFSREVDIMSSLRHKNITPFLGICIVDNKLISVYDFFSKGSLEANLYGRNKE 420

Query: 421 KGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWG 480
           K ILSW VRF+LA+GIAE LNYLH+E PRPVVHRDVKTSNILL+DELEP+LSDFGLAIWG
Sbjct: 421 KNILSWEVRFRLAIGIAEGLNYLHDECPRPVVHRDVKTSNILLSDELEPKLSDFGLAIWG 480

Query: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQ 540
           PTESSFQIEADVVGTFGYLAPEYFMYGKMS+K+DVYAFGIVLLELLSGRRAI  ETSKEQ
Sbjct: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSNKIDVYAFGIVLLELLSGRRAISAETSKEQ 540

Query: 541 QSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKV 600
           QSLVMWAKPIIE G+VK IVDPNLEG+FD  QL+RMVLAATLCI RASRLRPRI+QI+K+
Sbjct: 541 QSLVMWAKPIIESGNVKDIVDPNLEGRFDKEQLQRMVLAATLCIMRASRLRPRISQILKI 600

Query: 601 LRGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVC 660
           LRGE DTE++ VEDSQSVENGDDEVYP+SSSELHL+LALL VDD GGDSF+SVEQ KR+ 
Sbjct: 601 LRGESDTETLPVEDSQSVENGDDEVYPNSSSELHLNLALLDVDDDGGDSFNSVEQTKRLS 660

Query: 661 LEDYLKERWSRSSSFN 675
           LE Y KERWSRSSSF+
Sbjct: 661 LEKYFKERWSRSSSFD 675

BLAST of MC02g0845 vs. NCBI nr
Match: XP_038902069.1 (receptor-like cytoplasmic kinase 176 isoform X1 [Benincasa hispida] >XP_038902070.1 receptor-like cytoplasmic kinase 176 isoform X1 [Benincasa hispida])

HSP 1 Score: 1082 bits (2799), Expect = 0.0
Identity = 550/676 (81.36%), Postives = 607/676 (89.79%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTV Q+DSE IEK+KKKKKK  NVLVGIRINGD RDLLNWAIVKVADPGDCVIVIHVCQ+
Sbjct: 1   MTVDQDDSEAIEKEKKKKKK--NVLVGIRINGDSRDLLNWAIVKVADPGDCVIVIHVCQS 60

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRA K KPLFDEFLEGYKSLC+VNKVA ++ I TGSSVK TLVR+A+ Y A AV+LGTS
Sbjct: 61  SDRASKDKPLFDEFLEGYKSLCDVNKVALVALILTGSSVKKTLVRQAKSYAAGAVVLGTS 120

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           K S NLGGWS+TTRYFVKRLPPTTDVLAL  GK++FRR  +DQLPGL+ DPKPSFS A+Q
Sbjct: 121 KPS-NLGGWSSTTRYFVKRLPPTTDVLALNNGKIIFRRFTNDQLPGLNLDPKPSFSQASQ 180

Query: 181 SDFDGSETKKSFSYGVGSGDMKD-VHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTS 240
           SDFDGSET+KS SYGVGS D+KD VHGVVL+SKRNCSK D PMK ++SE GLGWPLLRT+
Sbjct: 181 SDFDGSETEKSVSYGVGSEDLKDEVHGVVLESKRNCSKPDPPMKMEHSERGLGWPLLRTT 240

Query: 241 PKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSER 300
           P+ISQT  VH+MSVVQWVMNLPDRSP  SL  KGNDPS+ EI  F+DER KGSLS+FSE 
Sbjct: 241 PRISQTPCVHNMSVVQWVMNLPDRSPYQSLSTKGNDPSKSEIPCFVDERAKGSLSSFSEP 300

Query: 301 PQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360
           P+ L+D LK +S+ YKWFSPDVLK STSHFSSENLIGKGGCN+VYKGILPDGK +AVKVM
Sbjct: 301 PEDLEDFLKTNSTNYKWFSPDVLKISTSHFSSENLIGKGGCNQVYKGILPDGKLVAVKVM 360

Query: 361 NSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKE 420
           NSS++AWNEFS+EVDIISSLHH+NITPFLG+C+ D+TLISVYDFFS+GSLEENL+  NK 
Sbjct: 361 NSSKQAWNEFSQEVDIISSLHHRNITPFLGICVVDNTLISVYDFFSKGSLEENLFDSNKG 420

Query: 421 KGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWG 480
           K ILSW VR +LA+G AE L YLH+E PRPVVHRDVKTSNILLTDELEP+LSDFGLAIWG
Sbjct: 421 KNILSWEVRVRLAIGTAEVLTYLHDECPRPVVHRDVKTSNILLTDELEPKLSDFGLAIWG 480

Query: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQ 540
           PTESSFQIEADVVGTFGYLAPEYFMYGKMS+K+DVYAFGIVLLELLSGRRAI  ETSKEQ
Sbjct: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSNKIDVYAFGIVLLELLSGRRAISAETSKEQ 540

Query: 541 QSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKV 600
           QSLVMWAKPIIE G+VK IVDPNL+GKFD  QL+RMVLAATLCITRASRLRPRI+QI+K+
Sbjct: 541 QSLVMWAKPIIESGNVKDIVDPNLDGKFDEEQLQRMVLAATLCITRASRLRPRISQILKI 600

Query: 601 LRGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVC 660
           LRGE DTES+ +EDSQSVENGDDEVYP+SSSELHLSLALLGVDDGG +SFSSVEQ KRV 
Sbjct: 601 LRGESDTESLPMEDSQSVENGDDEVYPNSSSELHLSLALLGVDDGG-NSFSSVEQTKRVA 660

Query: 661 LEDYLKERWSRSSSFN 675
           LEDY KERWSRSSSFN
Sbjct: 661 LEDYFKERWSRSSSFN 672

BLAST of MC02g0845 vs. NCBI nr
Match: XP_022944144.1 (probable serine/threonine-protein kinase PBL26 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 554/684 (80.99%), Postives = 603/684 (88.16%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTV  EDSE IEKKKKKKKK  NVLVGIRING+ RDLLNWAIVKVADPGDCVIVIHVCQ 
Sbjct: 1   MTVDHEDSEAIEKKKKKKKK--NVLVGIRINGNSRDLLNWAIVKVADPGDCVIVIHVCQT 60

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRA K KPLFDEF+EGYK LC+VNKVA I+ ISTGSSVK TLVR+A+ Y A AV+LGTS
Sbjct: 61  SDRASKDKPLFDEFIEGYKRLCDVNKVALIAQISTGSSVKKTLVRQAKSYAAGAVVLGTS 120

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           K S NLGGWS+T RY VKRLP TTDVLAL  GK+VFRRS +DQLPGL+ DPKPS S A+Q
Sbjct: 121 KPS-NLGGWSSTIRYVVKRLPRTTDVLALNNGKIVFRRSTNDQLPGLNLDPKPSLSQASQ 180

Query: 181 SDFDGSETKKSFSYGVGSGDMKD-VHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTS 240
           SDFDGSET+KS SYGVGS D+KD  HGVVL+SKR CSKQD+PMK  NSE GLGWPLLRTS
Sbjct: 181 SDFDGSETEKSVSYGVGSEDLKDEFHGVVLESKRVCSKQDSPMKKVNSEPGLGWPLLRTS 240

Query: 241 PKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSER 300
           P+I QT  VH+MSVVQWVMNLPDRSP HSL IKGNDPSR EIS          LSAFSE 
Sbjct: 241 PRIPQTPCVHNMSVVQWVMNLPDRSPFHSLSIKGNDPSRSEISG---------LSAFSEP 300

Query: 301 PQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360
           P  L+DLLK  S+ YKWFSPDVLK STSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM
Sbjct: 301 PGNLEDLLKTISTSYKWFSPDVLKASTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360

Query: 361 NSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKE 420
           N S++AWN+FSREVDIISSLHHKNITPFLG+CI+ DTLISVYDFFS+GSLEENLY RNKE
Sbjct: 361 NFSKQAWNDFSREVDIISSLHHKNITPFLGICIEHDTLISVYDFFSKGSLEENLYDRNKE 420

Query: 421 KGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWG 480
           KG+LSW VRF+ A+GIAEALNYLH+E P+PVVHRDVKTSNILLTDELEPQLSDFGLA WG
Sbjct: 421 KGVLSWEVRFEFAIGIAEALNYLHDECPQPVVHRDVKTSNILLTDELEPQLSDFGLATWG 480

Query: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQ 540
           PTESSF+IEADVVGTFGYLAPEYFMYGKMS+K+DVYAFGIVLLELLSGRRAI TETSKEQ
Sbjct: 481 PTESSFRIEADVVGTFGYLAPEYFMYGKMSNKIDVYAFGIVLLELLSGRRAISTETSKEQ 540

Query: 541 QSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKV 600
           QSLVMWAKP+IE G+VK IVDPNLEGKF++ QL+RMVLAATLCITRASRLRPRI+QI+K+
Sbjct: 541 QSLVMWAKPMIENGNVKNIVDPNLEGKFNDEQLQRMVLAATLCITRASRLRPRISQILKI 600

Query: 601 LRGE--IDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDG----GGDSFSSVE 660
           LRGE   +TE+I +EDSQSVENGDDEVYP+SSSELHLSLAL+GVDD     G DSFSSVE
Sbjct: 601 LRGESKTETENIHMEDSQSVENGDDEVYPNSSSELHLSLALVGVDDDNDNDGDDSFSSVE 660

Query: 661 QKKRVCLEDYLKERWSRSSSFNYL 677
           Q+KR+CLEDYLK RWSRSSSFN L
Sbjct: 661 QRKRICLEDYLKARWSRSSSFNQL 672

BLAST of MC02g0845 vs. NCBI nr
Match: KAG6571019.1 (Receptor-like cytosolic serine/threonine-protein kinase RBK1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1068 bits (2762), Expect = 0.0
Identity = 552/684 (80.70%), Postives = 601/684 (87.87%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTV  EDSE IEKKKKK     NVLVGIRING+ RDLLNWAIVKVADPGDCVIVIHVCQ 
Sbjct: 25  MTVDHEDSEAIEKKKKK-----NVLVGIRINGNSRDLLNWAIVKVADPGDCVIVIHVCQT 84

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRA K KPLFDEF+EGYK LC+VNKVA I+ ISTGSSVK TLVR+A+ Y A AV+LGTS
Sbjct: 85  SDRASKDKPLFDEFIEGYKRLCDVNKVALIAQISTGSSVKKTLVRQAKSYAAGAVVLGTS 144

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           K S NLGGWS+T RY VKRLP TTDVLAL  GK+VFRRS +DQLPGL+ DPKPS S A+Q
Sbjct: 145 KPS-NLGGWSSTIRYVVKRLPRTTDVLALNNGKIVFRRSTNDQLPGLNLDPKPSLSQASQ 204

Query: 181 SDFDGSETKKSFSYGVGSGDMKD-VHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTS 240
           SDFDGSET+KS SYGVGS D+KD  HGVVL+SKR CSKQD+PMK  NSE GLGWPLLRTS
Sbjct: 205 SDFDGSETEKSVSYGVGSEDLKDEFHGVVLESKRVCSKQDSPMKKVNSEPGLGWPLLRTS 264

Query: 241 PKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSER 300
           P+I QT  VH+MSVVQWVMNLPDRSP HSL IKGNDPSR EIS         SLSAFSE 
Sbjct: 265 PRIPQTPCVHNMSVVQWVMNLPDRSPFHSLSIKGNDPSRSEIS---------SLSAFSEP 324

Query: 301 PQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360
           P  L+DLLK  S+ YKWFSPDVLK STSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM
Sbjct: 325 PGNLEDLLKTISTSYKWFSPDVLKASTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 384

Query: 361 NSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKE 420
           N S++AWN+FSREVDIISSLHHKNITPFLG+CI+ DTLISVYDFFS+GSLEENLY RNKE
Sbjct: 385 NFSKQAWNDFSREVDIISSLHHKNITPFLGICIEHDTLISVYDFFSKGSLEENLYDRNKE 444

Query: 421 KGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWG 480
           KG+LSW VRF+ A+GIAEALNYLH+E P+PVVHRDVKTSNILLTDELEPQLSDFGLA WG
Sbjct: 445 KGVLSWEVRFEFAIGIAEALNYLHDECPQPVVHRDVKTSNILLTDELEPQLSDFGLATWG 504

Query: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQ 540
           PTESSF+IEADVVGTFGYLAPEYFMYGKMS+K+DVYAFGIVLLELLSGRRAI TETSKEQ
Sbjct: 505 PTESSFRIEADVVGTFGYLAPEYFMYGKMSNKIDVYAFGIVLLELLSGRRAISTETSKEQ 564

Query: 541 QSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKV 600
           QSLVMWAKP+IE G+VK IVDPNLEGKF++ QL+RMVLAATLCITRASRLRPRI+QI+K+
Sbjct: 565 QSLVMWAKPMIESGNVKNIVDPNLEGKFNDEQLQRMVLAATLCITRASRLRPRISQILKI 624

Query: 601 LRGE--IDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDG----GGDSFSSVE 660
           LRGE   +TE+I +EDSQSVENGDDEVYP+SSSELHLSLAL+GVDD     G DSFSSVE
Sbjct: 625 LRGESKTETENIHMEDSQSVENGDDEVYPNSSSELHLSLALVGVDDDNDNDGDDSFSSVE 684

Query: 661 QKKRVCLEDYLKERWSRSSSFNYL 677
           Q+KR+CLEDYLK RWSRSSSFN L
Sbjct: 685 QRKRICLEDYLKARWSRSSSFNQL 693

BLAST of MC02g0845 vs. ExPASy TrEMBL
Match: A0A6J1CWF0 (probable serine/threonine-protein kinase PBL23 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015423 PE=4 SV=1)

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 678/678 (100.00%), Postives = 678/678 (100.00%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN
Sbjct: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS
Sbjct: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ
Sbjct: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180

Query: 181 SDFDGSETKKSFSYGVGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTSP 240
           SDFDGSETKKSFSYGVGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTSP
Sbjct: 181 SDFDGSETKKSFSYGVGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTSP 240

Query: 241 KISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSERP 300
           KISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSERP
Sbjct: 241 KISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSERP 300

Query: 301 QKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMN 360
           QKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMN
Sbjct: 301 QKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMN 360

Query: 361 SSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEK 420
           SSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEK
Sbjct: 361 SSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEK 420

Query: 421 GILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGP 480
           GILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGP
Sbjct: 421 GILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGP 480

Query: 481 TESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQ 540
           TESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQ
Sbjct: 481 TESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQ 540

Query: 541 SLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVL 600
           SLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVL
Sbjct: 541 SLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVL 600

Query: 601 RGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVCL 660
           RGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVCL
Sbjct: 601 RGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVCL 660

Query: 661 EDYLKERWSRSSSFNYLP 678
           EDYLKERWSRSSSFNYLP
Sbjct: 661 EDYLKERWSRSSSFNYLP 678

BLAST of MC02g0845 vs. ExPASy TrEMBL
Match: A0A1S3C5P1 (serine/threonine-protein kinase At3g07070 OS=Cucumis melo OX=3656 GN=LOC103497332 PE=4 SV=1)

HSP 1 Score: 1083 bits (2800), Expect = 0.0
Identity = 547/676 (80.92%), Postives = 608/676 (89.94%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTV QEDSE IE++KK KK +KNVLVGIR+NGD RDLLNW+IVKVADPGDCVIVI+VCQ+
Sbjct: 1   MTVDQEDSEAIEEEKKTKKTRKNVLVGIRMNGDSRDLLNWSIVKVADPGDCVIVIYVCQS 60

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRA K KPLFDEFLEGY++LC+VNKVAFI+ I TGSSVK TLVR+A+ Y A AV+LGTS
Sbjct: 61  SDRASKDKPLFDEFLEGYRNLCDVNKVAFIAQIVTGSSVKKTLVRQAKIYAAGAVVLGTS 120

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           K   NLGGWS+ TRYFVKRLPPTTDVLAL  GK+VFRRS +DQL GLS DPKPSFS A+Q
Sbjct: 121 K-PCNLGGWSSITRYFVKRLPPTTDVLALNNGKIVFRRSTNDQLTGLSLDPKPSFSQASQ 180

Query: 181 SDFDGSETKKSFSYGVGSGDMKD-VHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTS 240
           SDFDGSET+KS SYGVGS D+KD V GVVL+SKRNCS+ D+PM  ++SE GLGWPLLRT+
Sbjct: 181 SDFDGSETEKSVSYGVGSEDLKDEVDGVVLESKRNCSEPDSPMMMEHSEPGLGWPLLRTT 240

Query: 241 PKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSER 300
           P+ISQT SVH+MSVVQWVMNLPDRSP  SL IKGNDP R EI  F+DER KGSLS+FSE 
Sbjct: 241 PRISQTSSVHNMSVVQWVMNLPDRSPHRSLSIKGNDPLRSEIPVFVDERAKGSLSSFSEP 300

Query: 301 PQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360
           P+ LKDLLK +S+ YKWFSP VLKTSTSHFSSENLIGKGGCNRVYKGILP+GKP+AVKVM
Sbjct: 301 PEDLKDLLKTNSTTYKWFSPYVLKTSTSHFSSENLIGKGGCNRVYKGILPNGKPVAVKVM 360

Query: 361 NSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKE 420
           NSS++AWNEFSREVDI+SSL HKNITPFLG+CI D+ LISVYDFFS+GSLE NLYGRNKE
Sbjct: 361 NSSKQAWNEFSREVDIMSSLRHKNITPFLGICIVDNKLISVYDFFSKGSLEANLYGRNKE 420

Query: 421 KGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWG 480
           K ILSW VRF+LA+GIAE LNYLH+E PRPVVHRDVKTSNILL+DELEP+LSDFGLAIWG
Sbjct: 421 KNILSWEVRFRLAIGIAEGLNYLHDECPRPVVHRDVKTSNILLSDELEPKLSDFGLAIWG 480

Query: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQ 540
           PTESSFQIEADVVGTFGYLAPEYFMYGKMS+K+DVYAFGIVLLELLSGRRAI  ETSKEQ
Sbjct: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSNKIDVYAFGIVLLELLSGRRAISAETSKEQ 540

Query: 541 QSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKV 600
           QSLVMWAKPIIE G+VK IVDPNLEG+FD  QL+RMVLAATLCI RASRLRPRI+QI+K+
Sbjct: 541 QSLVMWAKPIIESGNVKDIVDPNLEGRFDKEQLQRMVLAATLCIMRASRLRPRISQILKI 600

Query: 601 LRGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVC 660
           LRGE DTE++ VEDSQSVENGDDEVYP+SSSELHL+LALL VDD GGDSF+SVEQ KR+ 
Sbjct: 601 LRGESDTETLPVEDSQSVENGDDEVYPNSSSELHLNLALLDVDDDGGDSFNSVEQTKRLS 660

Query: 661 LEDYLKERWSRSSSFN 675
           LE Y KERWSRSSSF+
Sbjct: 661 LEKYFKERWSRSSSFD 675

BLAST of MC02g0845 vs. ExPASy TrEMBL
Match: A0A6J1FW59 (probable serine/threonine-protein kinase PBL26 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448689 PE=4 SV=1)

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 554/684 (80.99%), Postives = 603/684 (88.16%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTV  EDSE IEKKKKKKKK  NVLVGIRING+ RDLLNWAIVKVADPGDCVIVIHVCQ 
Sbjct: 1   MTVDHEDSEAIEKKKKKKKK--NVLVGIRINGNSRDLLNWAIVKVADPGDCVIVIHVCQT 60

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRA K KPLFDEF+EGYK LC+VNKVA I+ ISTGSSVK TLVR+A+ Y A AV+LGTS
Sbjct: 61  SDRASKDKPLFDEFIEGYKRLCDVNKVALIAQISTGSSVKKTLVRQAKSYAAGAVVLGTS 120

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           K S NLGGWS+T RY VKRLP TTDVLAL  GK+VFRRS +DQLPGL+ DPKPS S A+Q
Sbjct: 121 KPS-NLGGWSSTIRYVVKRLPRTTDVLALNNGKIVFRRSTNDQLPGLNLDPKPSLSQASQ 180

Query: 181 SDFDGSETKKSFSYGVGSGDMKD-VHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTS 240
           SDFDGSET+KS SYGVGS D+KD  HGVVL+SKR CSKQD+PMK  NSE GLGWPLLRTS
Sbjct: 181 SDFDGSETEKSVSYGVGSEDLKDEFHGVVLESKRVCSKQDSPMKKVNSEPGLGWPLLRTS 240

Query: 241 PKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSER 300
           P+I QT  VH+MSVVQWVMNLPDRSP HSL IKGNDPSR EIS          LSAFSE 
Sbjct: 241 PRIPQTPCVHNMSVVQWVMNLPDRSPFHSLSIKGNDPSRSEISG---------LSAFSEP 300

Query: 301 PQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360
           P  L+DLLK  S+ YKWFSPDVLK STSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM
Sbjct: 301 PGNLEDLLKTISTSYKWFSPDVLKASTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360

Query: 361 NSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKE 420
           N S++AWN+FSREVDIISSLHHKNITPFLG+CI+ DTLISVYDFFS+GSLEENLY RNKE
Sbjct: 361 NFSKQAWNDFSREVDIISSLHHKNITPFLGICIEHDTLISVYDFFSKGSLEENLYDRNKE 420

Query: 421 KGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWG 480
           KG+LSW VRF+ A+GIAEALNYLH+E P+PVVHRDVKTSNILLTDELEPQLSDFGLA WG
Sbjct: 421 KGVLSWEVRFEFAIGIAEALNYLHDECPQPVVHRDVKTSNILLTDELEPQLSDFGLATWG 480

Query: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQ 540
           PTESSF+IEADVVGTFGYLAPEYFMYGKMS+K+DVYAFGIVLLELLSGRRAI TETSKEQ
Sbjct: 481 PTESSFRIEADVVGTFGYLAPEYFMYGKMSNKIDVYAFGIVLLELLSGRRAISTETSKEQ 540

Query: 541 QSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKV 600
           QSLVMWAKP+IE G+VK IVDPNLEGKF++ QL+RMVLAATLCITRASRLRPRI+QI+K+
Sbjct: 541 QSLVMWAKPMIENGNVKNIVDPNLEGKFNDEQLQRMVLAATLCITRASRLRPRISQILKI 600

Query: 601 LRGE--IDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDG----GGDSFSSVE 660
           LRGE   +TE+I +EDSQSVENGDDEVYP+SSSELHLSLAL+GVDD     G DSFSSVE
Sbjct: 601 LRGESKTETENIHMEDSQSVENGDDEVYPNSSSELHLSLALVGVDDDNDNDGDDSFSSVE 660

Query: 661 QKKRVCLEDYLKERWSRSSSFNYL 677
           Q+KR+CLEDYLK RWSRSSSFN L
Sbjct: 661 QRKRICLEDYLKARWSRSSSFNQL 672

BLAST of MC02g0845 vs. ExPASy TrEMBL
Match: A0A5D3BJR4 (Serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001110 PE=4 SV=1)

HSP 1 Score: 1067 bits (2760), Expect = 0.0
Identity = 545/683 (79.80%), Postives = 605/683 (88.58%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHV--- 60
           MTV QEDSE IE++KK KK +KNVLVGIR+NGD RDLLNW+IVKVADPGDCVIVI+    
Sbjct: 1   MTVDQEDSEAIEEEKKTKKTRKNVLVGIRMNGDSRDLLNWSIVKVADPGDCVIVIYFWNV 60

Query: 61  ----CQNSDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDAR 120
               CQ  DRA K KPLFDEFLEGY++LC+VNKVAFI+ I TGSSVK TLVR+A+ Y A 
Sbjct: 61  IFPSCQT-DRASKDKPLFDEFLEGYRNLCDVNKVAFIAQIVTGSSVKKTLVRQAKIYAAG 120

Query: 121 AVILGTSKLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKP 180
           AV+LGTSK   NLGGWS+ TRYFVKRLPPTTDVLAL  GK+VFRRS +DQL GLS DPKP
Sbjct: 121 AVVLGTSK-PCNLGGWSSITRYFVKRLPPTTDVLALNNGKIVFRRSTNDQLTGLSLDPKP 180

Query: 181 SFSPATQSDFDGSETKKSFSYGVGSGDMKD-VHGVVLKSKRNCSKQDTPMKTDNSEGGLG 240
           SFS A+QSDFDGSET+KS SYGVGS D+KD V GVVL+SKRNCS+ D+PM  ++SE GLG
Sbjct: 181 SFSQASQSDFDGSETEKSVSYGVGSEDLKDEVDGVVLESKRNCSEPDSPMMMEHSEPGLG 240

Query: 241 WPLLRTSPKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGS 300
           WPLLRT+P+ISQT SVH+MSVVQWVMNLPDRSP  SL IKGNDP R EI  F+DER KGS
Sbjct: 241 WPLLRTTPRISQTSSVHNMSVVQWVMNLPDRSPHRSLSIKGNDPLRSEIPVFVDERAKGS 300

Query: 301 LSAFSERPQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGK 360
           LS+FSE P+ LKDLLK +S+ YKWFSP VLKTSTSHFSSENLIGKGGCNRVYKGILP+GK
Sbjct: 301 LSSFSEPPEDLKDLLKTNSTTYKWFSPYVLKTSTSHFSSENLIGKGGCNRVYKGILPNGK 360

Query: 361 PIAVKVMNSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEEN 420
           P+AVKVMNSS++AWNEFSREVDI+SSL HKNITPFLG+CI D+ LISVYDFFS+GSLE N
Sbjct: 361 PVAVKVMNSSKQAWNEFSREVDIMSSLRHKNITPFLGICIVDNKLISVYDFFSKGSLEAN 420

Query: 421 LYGRNKEKGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSD 480
           LYGRNKEK ILSW VRF+LA+GIAE LNYLH+E PRPVVHRDVKTSNILL+DELEP+LSD
Sbjct: 421 LYGRNKEKNILSWEVRFRLAIGIAEGLNYLHDECPRPVVHRDVKTSNILLSDELEPKLSD 480

Query: 481 FGLAIWGPTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAID 540
           FGLAIWGPTESSFQIEADVVGTFGYLAPEYFMYGKMS+K+DVYAFGIVLLELLSGRRAI 
Sbjct: 481 FGLAIWGPTESSFQIEADVVGTFGYLAPEYFMYGKMSNKIDVYAFGIVLLELLSGRRAIS 540

Query: 541 TETSKEQQSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPR 600
            ETSKEQQSLVMWAKPIIE G+VK IVDPNLEG+FD  QL+RMVLAATLCI RASRLRPR
Sbjct: 541 AETSKEQQSLVMWAKPIIESGNVKDIVDPNLEGRFDKEQLQRMVLAATLCIMRASRLRPR 600

Query: 601 ITQIMKVLRGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSV 660
           I+QI+K+LRGE DTE++ VEDSQSVENGDDEVYP+SSSELHL+LALL VDD GGDSF+SV
Sbjct: 601 ISQILKILRGESDTETLPVEDSQSVENGDDEVYPNSSSELHLNLALLDVDDDGGDSFNSV 660

Query: 661 EQKKRVCLEDYLKERWSRSSSFN 675
           EQ KR+ LE Y KERWSRSSSF+
Sbjct: 661 EQTKRLSLEKYFKERWSRSSSFD 681

BLAST of MC02g0845 vs. ExPASy TrEMBL
Match: A0A0A0LJ74 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270770 PE=4 SV=1)

HSP 1 Score: 1056 bits (2730), Expect = 0.0
Identity = 534/676 (78.99%), Postives = 604/676 (89.35%), Query Frame = 0

Query: 1   MTVGQEDSEDIEKKKKKKKKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQN 60
           MTV QEDSE IE++KKKK +K NVLVGIR+NGD RDLLNW+IVKVADPGDCVIVI+VCQ+
Sbjct: 1   MTVDQEDSEAIEEEKKKKTRK-NVLVGIRMNGDSRDLLNWSIVKVADPGDCVIVIYVCQS 60

Query: 61  SDRALKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTS 120
           SDRA K KPLFDEFLEGY+SLC+VNKV FI+H+ TGSSVK TLVR+A+ Y A AV+LGTS
Sbjct: 61  SDRASKDKPLFDEFLEGYRSLCDVNKVTFIAHMVTGSSVKKTLVRQAKIYAAGAVVLGTS 120

Query: 121 KLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQ 180
           K   NLGGWS+ TRYFVKRLPPTT++L L  GK+VFRRS +DQL GLS DPKPSFS A+Q
Sbjct: 121 K-PCNLGGWSSITRYFVKRLPPTTNILVLNNGKIVFRRSTNDQLTGLSLDPKPSFSQASQ 180

Query: 181 SDFDGSETKKSFSYGVGSGDMKD-VHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLRTS 240
           SDFDGSET+KS SYGVGS D+KD V GVVL+SKRNCSK D+ M  ++SE GLGWPLLRT+
Sbjct: 181 SDFDGSETEKSVSYGVGSEDLKDEVDGVVLESKRNCSKPDSAMMMEHSEPGLGWPLLRTT 240

Query: 241 PKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSER 300
           P+ISQT SVH+MSVVQWVMNLPDRSP  SL I  NDPS+ EI   +  R KG+LS+FSE 
Sbjct: 241 PRISQTSSVHNMSVVQWVMNLPDRSPHRSLSITVNDPSKSEIHRKV--RAKGNLSSFSEL 300

Query: 301 PQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVM 360
           P+ L+DLLK +S+ YKWFSP VLKTSTSHFSSENLIGKGGCN VYKGILP+GKP+AVKVM
Sbjct: 301 PEDLEDLLKTNSTTYKWFSPYVLKTSTSHFSSENLIGKGGCNLVYKGILPNGKPVAVKVM 360

Query: 361 NSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKE 420
           NSS++AW+EF REVDI+SSLHHKNI+PFLG+CI D+ LISVYDFFS+GSLE NLYGRNKE
Sbjct: 361 NSSKQAWDEFFREVDIMSSLHHKNISPFLGICIADNKLISVYDFFSKGSLEANLYGRNKE 420

Query: 421 KGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWG 480
           K ILSW VRF+LA+GIAEALNYLH+E PRPVVHRDVKTSNILL+DELEP+LSDFGLAIWG
Sbjct: 421 KNILSWEVRFRLAIGIAEALNYLHDECPRPVVHRDVKTSNILLSDELEPKLSDFGLAIWG 480

Query: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQ 540
           PTESSFQIEADVVGTFGYLAPEYFMYGKMS+K+DVYAFGIVLLELLSGR+AI  ETSKEQ
Sbjct: 481 PTESSFQIEADVVGTFGYLAPEYFMYGKMSNKIDVYAFGIVLLELLSGRKAISAETSKEQ 540

Query: 541 QSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKV 600
           QSLVMWAKPI E G+VK IVDPNLEGKFD  QL+RM+LAATLCITRASR+RPRI+QI+K+
Sbjct: 541 QSLVMWAKPITESGNVKDIVDPNLEGKFDEEQLQRMILAATLCITRASRIRPRISQILKI 600

Query: 601 LRGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQKKRVC 660
           LRGE DTE++ VEDSQSVENGDDEVYP+SSSELHL+LALLGVDD GGDSF+S+EQKK++ 
Sbjct: 601 LRGESDTETLPVEDSQSVENGDDEVYPNSSSELHLNLALLGVDDDGGDSFNSMEQKKKLT 660

Query: 661 LEDYLKERWSRSSSFN 675
           LE Y KERWSRSSSFN
Sbjct: 661 LEKYFKERWSRSSSFN 672

BLAST of MC02g0845 vs. TAIR 10
Match: AT2G16750.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 534.3 bits (1375), Expect = 1.5e-151
Identity = 303/643 (47.12%), Postives = 409/643 (63.61%), Query Frame = 0

Query: 19  KKKKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQNSDRALKTKPLFDEFLEGY 78
           K++  +LVGI I+  G ++L WA+ +VA  GDCV+V+HVC    RALK+K   D +L+ Y
Sbjct: 9   KQRNIILVGIPIDESGVEVLKWALEEVAKHGDCVVVVHVCFTYYRALKSKSSLDRYLKPY 68

Query: 79  KSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGT---SKLSTNLGGWSTTTRY 138
              C+  K+     +  G+SV   LV+EA+ Y+A +V++G    SKLS  +       + 
Sbjct: 69  IEFCSTKKIELKGEVLKGNSVLGVLVKEAKRYNAMSVVVGVKQQSKLSLKI------AKG 128

Query: 139 FVKRLPPTTDVLALYKGKVVFRRSISDQLPGLSQDPKPSFSPATQSDFDGSETKKSFSYG 198
             K LP TTD+LA+++G +VFRRS   QLP L+Q  K S  P+       SE  + FS  
Sbjct: 129 CAKELPSTTDILAIHRGNIVFRRSNHYQLP-LAQ--KISSRPS-------SELSEGFS-- 188

Query: 199 VGSGDMKDVHGVVLKSKRNCSKQDTPM-KTDNSEGGLGWPLLRTSPKISQTL--SVHDMS 258
                 KD+     + KR  S +   +   +  +   GWPLLRTS   +  +      +S
Sbjct: 189 -----DKDLAKTTGQEKRKISGRSLSLPSVEVVDQTPGWPLLRTSTLATPMVQHQTRKIS 248

Query: 259 VVQWVMNLPDRSPSHSLRIKGNDPSRREISDFLDERNKGSLSAFSERPQKLKDLLKASSS 318
           VV WVM+LP+R P H        P++     F D              ++LKD+LK    
Sbjct: 249 VVNWVMSLPERFPHH--------PNQTCQQSFCD--------------KQLKDILK---D 308

Query: 319 CYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMNSSEK-AWNEFSR 378
             +WFS DVLKT+TS FS ENLIGKGGCN VYKG L DGK +AVK++  S K A  EF  
Sbjct: 309 INRWFSYDVLKTATSDFSLENLIGKGGCNEVYKGFLEDGKGVAVKILKPSVKEAVKEFVH 368

Query: 379 EVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEKGILSWVVRFKL 438
           EV I+SSL H NI+P +GVC+  + LISVY+  S+GSLEE L G    K +L W  R K+
Sbjct: 369 EVSIVSSLSHSNISPLIGVCVHYNDLISVYNLSSKGSLEETLQG----KHVLRWEERLKI 428

Query: 439 AVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGPTESSFQIEADV 498
           A+G+ EAL+YLH +   PV+HRDVK+SN+LL+DE EPQLSDFGL++WG     + I+ DV
Sbjct: 429 AIGLGEALDYLHNQCSNPVIHRDVKSSNVLLSDEFEPQLSDFGLSMWGSKSCRYTIQRDV 488

Query: 499 VGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQSLVMWAKPIIE 558
           VGTFGYLAPEYFMYGK+SDKVDVYAFG+VLLEL+SGR +I +++ + Q+SLVMWAKP+IE
Sbjct: 489 VGTFGYLAPEYFMYGKVSDKVDVYAFGVVLLELISGRTSISSDSPRGQESLVMWAKPMIE 548

Query: 559 RGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLRPRITQIMKVLRGEIDTESIVV 618
           +G+ K ++DPN+ G FD  Q  +MVLAAT C+TRA+  RP I +I+K+LRGE D    V 
Sbjct: 549 KGNAKELLDPNIAGTFDEDQFHKMVLAATHCLTRAATYRPNIKEILKLLRGEDDVSKWVK 599

Query: 619 EDSQSVENGDDEVYPSSSSELHLSLALLGVDDGGGDSFSSVEQ 655
            +    +  DDEVYP+S++ELHLSLA++ V+D    S SS+E+
Sbjct: 609 IEEDDEDGFDDEVYPNSNTELHLSLAMVDVEDNDSVSNSSLER 599

BLAST of MC02g0845 vs. TAIR 10
Match: AT1G77280.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 444.9 bits (1143), Expect = 1.2e-124
Identity = 288/776 (37.11%), Postives = 422/776 (54.38%), Query Frame = 0

Query: 6   EDSEDIEKKKKKKKK------KKNVLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQ 65
           E SE IE    K+         + +LVG++++   R+LL WA+VKVA+PGD VI +H+  
Sbjct: 26  EQSEMIETGGDKQSSVEEGCGGRTILVGVKLDAPSRELLTWALVKVAEPGDTVIALHILG 85

Query: 66  NS--DRA-----LKTKPLFDEFLEGYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDA 125
           N   DRA     L     FD  L+ Y+  CN+ +V     +  GSS +  LVREA+ + A
Sbjct: 86  NEIVDRAGNSSLLSLVRTFDSVLDVYEGFCNLKQVDLKLKLCRGSSARKILVREAKSFSA 145

Query: 126 RAVILGTSKLSTNLGGWSTTTRYFVKRLPPTTDVLALYKGKVVFRRS------------I 185
             V++G SK    +   ++  +Y  K+L     V+A+  GK++F++             +
Sbjct: 146 TKVLVGISKSHHAIRSSASVAKYIAKKLSKDCWVIAVNNGKILFQKEGSPSSTINQSQVV 205

Query: 186 SDQLPGLSQDPKPSFSPAT-----QSDFDGSETKKSFSYGVGSGDMKDVH---------G 245
            + + G     K      T     Q     ++T K  S+     + K+            
Sbjct: 206 EETVIGFGYIGKEDVRRITLLNVLQRSVTLNKTTKVVSHSEEDSNSKEEEEDQACGQNLR 265

Query: 246 VVLKSKR--NC--------SKQDT----------------------------PMKTDNSE 305
            VL + R  NC        S  DT                            P+      
Sbjct: 266 QVLAAARLENCSVCGFDSLSPNDTTTPGKLSGASDFDRSEDDDECNKAMEIVPVNGSEDS 325

Query: 306 GGL-------------GWPLLRTS-PKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIKGN 365
           GG              GWPLLR +   + Q+++ H + VVQW + LP R         G 
Sbjct: 326 GGSVTMLVRKLPEPRPGWPLLRRAVSTLGQSVTPHKIPVVQWALKLPPRDTKQ----LGY 385

Query: 366 DPSRREISDF-------LDERNKGSLSAFSER--PQKLKDLLKASSSCYKWFSPDVLKTS 425
           D S   +S         ++     S+   S R  P++L+ L +  SS  ++F    L + 
Sbjct: 386 DSSEDNLSTLNALVPFGINSITNKSIPDNSPRKLPEELEGLYERFSSTCRFFKYKELVSV 445

Query: 426 TSHFSSENLIGKGGCNRVYKGILPDGKPIAVKVMNSSEKAWNEFSREVDIISSLHHKNIT 485
           TS+FS++N IGKGG +RV++G L +G+ +AVK++  +E   N+F  E++II++LHHKNI 
Sbjct: 446 TSNFSADNFIGKGGSSRVFRGCLSNGRVVAVKILKQTEDVLNDFVAEIEIITTLHHKNII 505

Query: 486 PFLGVCIKDDTLISVYDFFSQGSLEENLYGRNKEKGILSWVVRFKLAVGIAEALNYLHEE 545
             LG C +D  L+ VY++ S+GSLEENL+G  K+     W  R+K+AVG+AEAL+YLH  
Sbjct: 506 SLLGFCFEDHNLLLVYNYLSRGSLEENLHGNKKDPLAFCWSERYKVAVGVAEALDYLHNT 565

Query: 546 SPRPVVHRDVKTSNILLTDELEPQLSDFGLAIWGPTESSFQIEADVVGTFGYLAPEYFMY 605
           + +PV+HRDVK+SNILL+D+ EPQLSDFGLA W    ++  I +DV GTFGYLAPEYFMY
Sbjct: 566 ASQPVIHRDVKSSNILLSDDFEPQLSDFGLARWASISTTHIICSDVAGTFGYLAPEYFMY 625

Query: 606 GKMSDKVDVYAFGIVLLELLSGRRAIDTETSKEQQSLVMWAKPIIERGDVKGIVDPNLEG 665
           GK++DK+DVYAFG+VLLELLSGR+ I +   K Q+SLVMWAKPI++ G    ++DP+L  
Sbjct: 626 GKVNDKIDVYAFGVVLLELLSGRKPISSGCPKGQESLVMWAKPILDDGKYSQLLDPSLRD 685

Query: 666 KFDN--VQLKRMVLAATLCITRASRLRPRITQIMKVLRGEIDTESIVVE----DSQSVEN 676
             +N   Q++RM LAATLCI R+ + RP+++ ++K+L+G+ DT    ++     S+  E 
Sbjct: 686 NNNNNDDQMQRMALAATLCIRRSPQARPKMSIVLKLLKGDEDTLEWAMQQVNSSSEESEM 745

BLAST of MC02g0845 vs. TAIR 10
Match: AT4G35030.3 (Protein kinase superfamily protein )

HSP 1 Score: 431.8 bits (1109), Expect = 1.0e-120
Identity = 221/430 (51.40%), Postives = 301/430 (70.00%), Query Frame = 0

Query: 232 GWPLLRT----SPKISQTLSVHDMSVVQWVMNLPDRSPSHSLRIK-GNDPSRREISDFLD 291
           GWP L+     +P+         +SVV WVM+LP+R P+H   +       +++I D L 
Sbjct: 27  GWPFLKRASLGTPQQVHKWHTRKVSVVNWVMSLPERFPNHQQTLNYETSLIKKQIKDILR 86

Query: 292 ERNKGSLSAFSERPQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKG 351
           + N                         KWF+ +VL+ +TS FS EN+IGKGGCN VY+G
Sbjct: 87  DNN-------------------------KWFNYNVLRKATSDFSQENVIGKGGCNEVYRG 146

Query: 352 ILPDGKPIAVKVMNSSEK-AWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFS 411
           IL DGK IAVK++ SS K A   F  E++IISSL H+NI+P LGVC++D+ LISVY+  +
Sbjct: 147 ILEDGKGIAVKILKSSSKEAMTNFVHEINIISSLSHQNISPLLGVCVQDNELISVYNLSN 206

Query: 412 QGSLEENLYGRNKEKGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDE 471
            GSLEE L+G+ K K +LSW  RFK+A+G+AEAL+YLH    +PV+HRDVKTSN+LL+ E
Sbjct: 207 TGSLEETLHGKQKGKYVLSWEERFKIAIGLAEALDYLHNRCSKPVIHRDVKTSNVLLSLE 266

Query: 472 LEPQLSDFGLAIWGPTESS-FQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLEL 531
           L+PQLSDFGL++WGPT SS + I+ DVVGTFGYLAPEYFMYGK+SDKVDVYAFG+VLLEL
Sbjct: 267 LQPQLSDFGLSMWGPTTSSRYSIQGDVVGTFGYLAPEYFMYGKVSDKVDVYAFGVVLLEL 326

Query: 532 LSGRRAIDTETSKEQQSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCIT 591
           +SGR  I  +  + Q+SLVMWAKP+I+ G++K ++DP++   FD  Q +RMVLAA+ C+T
Sbjct: 327 ISGRNPISPQNPRGQESLVMWAKPLIDTGNLKVLLDPDVTDIFDESQFQRMVLAASHCLT 386

Query: 592 RASRLRPRITQIMKVLRGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLALLGVDDG 651
           R++  RP I QI+++LR E +    ++E+  + +  DDEVYP+SS+ELHL+LA+L V+D 
Sbjct: 387 RSATHRPNIRQILRLLRDENEAGKWIMEEEGNEDCFDDEVYPNSSTELHLNLAMLEVEDD 431

Query: 652 GGDSFSSVEQ 655
              S SS+E+
Sbjct: 447 ETASISSMER 431

BLAST of MC02g0845 vs. TAIR 10
Match: AT1G21590.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 431.4 bits (1108), Expect = 1.3e-120
Identity = 270/752 (35.90%), Postives = 418/752 (55.59%), Query Frame = 0

Query: 24  VLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHVCQNS--DRALKTKPL-----FDEFLE 83
           V+VG++ +    +LL+WA+VKVA+PGD VI +HV  N   DRA  +  +     FD  LE
Sbjct: 14  VMVGVKFDESSNELLDWALVKVAEPGDTVIALHVLGNEIVDRADNSSLVSIVKNFDSVLE 73

Query: 84  GYKSLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTSKLSTNLGGWSTTTRYF 143
            Y+  C + ++     +S GSS +  LV+EA+   A  V++G S+    +    +  +Y 
Sbjct: 74  VYEGFCKLKQLELKLKLSRGSSTRKILVKEAKMCSASKVVVGISRRFHTIHSSVSVAKYL 133

Query: 144 VKRLPPTTDVLALYKGKVVFRR----SISDQLPGLSQDPKPSFS---------------- 203
            +++     VLA+  GKV+F++    SI     G S   + + S                
Sbjct: 134 ARKVAKDCWVLAVDNGKVMFQKDGSSSIIHHSKGKSDARRNTLSSFFQMPVTLRKNTKVV 193

Query: 204 --------PATQSDFDGSETKKSFSYG-VGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNS 263
                    A +   +G   ++S  Y  +G+  ++D++ +      + S      + DN+
Sbjct: 194 NHSEVEEEEAEEDHSNGQSLRRSLVYACLGNCSVRDMNSLPTPGNLSRSSSCNGDQDDNA 253

Query: 264 -----------------------------EGGLGWPL---------LRTSPKISQTLSVH 323
                                        E   GWPL         L ++P+ S   S  
Sbjct: 254 DLHKAMALVPAKFPEDLTPFITMLVKELPEFRPGWPLLCRVASSDVLASAPRSS---SFR 313

Query: 324 DMSVVQWVMNLPDRS----PSHSLRIKGNDPSRREISDFLDERN--------------KG 383
            + VVQWV+ LP R+     S   +  G D S  E +D L   N              K 
Sbjct: 314 KIPVVQWVLKLPARTNSVVGSSDTKQIGFDSSESEENDKLSSSNVERQAIVPDESMIVKC 373

Query: 384 SLSAFSER-PQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKGGCNRVYKGILPD 443
           SL   S R P+ ++ L    S+  ++F+   L + TS+F ++N IGKGG +RV++G LP+
Sbjct: 374 SLDHSSGRFPENVEGLQARISTSCQFFTYKELVSVTSNFCADNFIGKGGSSRVFRGYLPN 433

Query: 444 GKPIAVKVMNSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLISVYDFFSQGSLE 503
           G+ +AVK++  +E    +F  E+DII++LHHKN+   LG C +++ L+ VY++ S+GSLE
Sbjct: 434 GREVAVKILKRTECVLKDFVAEIDIITTLHHKNVISLLGYCFENNNLLLVYNYLSRGSLE 493

Query: 504 ENLYGRNKEKGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTSNILLTDELEPQL 563
           ENL+G  K+     W  R+K+AVGIAEAL+YLH ++P+PV+HRDVK+SNILL+D+ EPQL
Sbjct: 494 ENLHGNKKDLVAFRWNERYKVAVGIAEALDYLHNDAPQPVIHRDVKSSNILLSDDFEPQL 553

Query: 564 SDFGLAIWGPTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFGIVLLELLSGRRA 623
           SDFGLA W    ++  I +DV GTFGYLAPEYFMYGKM++K+DVYA+G+VLLELLSGR+ 
Sbjct: 554 SDFGLAKWASESTTQIICSDVAGTFGYLAPEYFMYGKMNNKIDVYAYGVVLLELLSGRKP 613

Query: 624 IDTETSKEQQSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLAATLCITRASRLR 676
           +++E+ K Q SLVMWAKPI++  +   ++D +L+   ++ Q+++M LAATLCI    + R
Sbjct: 614 VNSESPKAQDSLVMWAKPILDDKEYSQLLDSSLQDDNNSDQMEKMALAATLCIRHNPQTR 673

BLAST of MC02g0845 vs. TAIR 10
Match: AT5G63940.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 413.7 bits (1062), Expect = 2.9e-115
Identity = 254/698 (36.39%), Postives = 388/698 (55.59%), Query Frame = 0

Query: 24  VLVGIRINGDGRDLLNWAIVKVADPGDCVIVIHV----CQNSDRALKTKPLFDEFLEGYK 83
           ++VG++ +   R++L W++V VA PGD ++ +HV     + S   +     FD  L  Y+
Sbjct: 22  LIVGVKPDEWSREVLTWSLVNVARPGDRIVALHVLDYSLEGSTSLISLVRNFDTMLGVYE 81

Query: 84  SLCNVNKVAFISHISTGSSVKTTLVREAECYDARAVILGTSKLSTNLGGWSTTTRYFVKR 143
           S CN+ +V     +  G S +  LV+E +   A ++I+G+S+    +   ++  +Y  + 
Sbjct: 82  SFCNLKQVDLKLKVFRGKSARKVLVQEVKSCGATSLIVGSSRKHHTIRSSASLAKYCARN 141

Query: 144 LPPTTDVLALYKGKVVFRR---------------SISDQLPGLSQDPKP---SFSPATQS 203
           L     V A+  GK++FRR               S+    P ++ +      SFSPA  S
Sbjct: 142 LAKDVSVFAVKSGKIMFRRVPNTNGADGPQMNIPSLVCGSPDIAIEAAKIGNSFSPARTS 201

Query: 204 DFDGSETKKSFSYGVGSGDMKDVHGVVLKSKRNCSKQDTPMKTDNSEGGLGWPLLR---T 263
                 T+ S S  + S D   V  ++       ++ D    + + E G GW  LR    
Sbjct: 202 ---SRWTRTSRSSSLQSPDSLGVDNLLALVPVQTNETD----SGSLESGPGWHFLRGLYG 261

Query: 264 SPKISQTLSVHDMSVVQWVMNLP----------DRSPSH-------SLRIKGNDPSRREI 323
           + + S T      +V+QWV  L           DR  S        S  I G D S   I
Sbjct: 262 NNRKSWTKVSAKKAVLQWVSRLRGRHSETVIYLDRKRSDSGCDEDCSSSIDGEDVS---I 321

Query: 324 SDFLDERNKGSLSAF---SERPQKLKDLLKASSSCYKWFSPDVLKTSTSHFSSENLIGKG 383
           S F  E  +  LS F   +  P++L+ L +  SS  + F+ + + + TS+F+SENL+G+G
Sbjct: 322 SRFGSELMQSPLSPFIGSNNIPEELEGLHEKYSSTCRLFTYEEVLSITSNFASENLVGEG 381

Query: 384 GCNRVYKGILPDGKPIAVKVMNSSEKAWNEFSREVDIISSLHHKNITPFLGVCIKDDTLI 443
           G + VY+G LPDG+ +AVK++        EF  E+++I+S+HHKNI    G C +++ L+
Sbjct: 382 GNSYVYRGDLPDGRELAVKILKPCLDVLKEFILEIEVITSVHHKNIVSLFGFCFENNNLM 441

Query: 444 SVYDFFSQGSLEENLYGRNKEKGILSWVVRFKLAVGIAEALNYLHEESPRPVVHRDVKTS 503
            VYD+  +GSLEENL+G  K+     W+ R+K+AVG+AEAL+YLH      V+HRDVK+S
Sbjct: 442 LVYDYLPRGSLEENLHGNRKDAKKFGWMERYKVAVGVAEALDYLHNTHDPEVIHRDVKSS 501

Query: 504 NILLTDELEPQLSDFGLAIWGPTESSFQIEADVVGTFGYLAPEYFMYGKMSDKVDVYAFG 563
           N+LL D+ EPQLSDFG A    + S      D+ GTFGYLAPEYFM+GK++DK+DVYAFG
Sbjct: 502 NVLLADDFEPQLSDFGFASLASSTSQHVAGGDIAGTFGYLAPEYFMHGKVTDKIDVYAFG 561

Query: 564 IVLLELLSGRRAIDTETSKEQQSLVMWAKPIIERGDVKGIVDPNLEGKFDNVQLKRMVLA 623
           +VLLEL+SGR+ I  + SK Q+SLV+WA PI++ G    ++DP+LE    N  +++++LA
Sbjct: 562 VVLLELISGRKPICVDQSKGQESLVLWANPILDSGKFAQLLDPSLENDNSNDLIEKLLLA 621

Query: 624 ATLCITRASRLRPRITQIMKVLRGEIDTESIVVEDSQSVENGDDEVYPSSSSELHLSLAL 677
           ATLCI R    RP+I  ++K+L+GE   E       Q V   +D     ++ E H++LAL
Sbjct: 622 ATLCIKRTPHDRPQIGLVLKILQGE---EEATEWGKQQVRASEDVSAYLTNIESHINLAL 681

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SGY71.0e-6439.69Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thalian... [more]
Q8RXC82.3e-6443.28Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thal... [more]
Q8H1D68.6e-6440.00Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thal... [more]
O655301.1e-6340.99Proline-rich receptor-like protein kinase PERK14 OS=Arabidopsis thaliana OX=3702... [more]
Q9LV489.5e-6338.61Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_022146129.10.0100.00probable serine/threonine-protein kinase PBL23 isoform X1 [Momordica charantia] ... [more]
XP_008457694.10.080.92PREDICTED: serine/threonine-protein kinase At3g07070 [Cucumis melo] >XP_00845769... [more]
XP_038902069.10.081.36receptor-like cytoplasmic kinase 176 isoform X1 [Benincasa hispida] >XP_03890207... [more]
XP_022944144.10.080.99probable serine/threonine-protein kinase PBL26 isoform X1 [Cucurbita moschata][more]
KAG6571019.10.080.70Receptor-like cytosolic serine/threonine-protein kinase RBK1, partial [Cucurbita... [more]
Match NameE-valueIdentityDescription
A0A6J1CWF00.0100.00probable serine/threonine-protein kinase PBL23 isoform X1 OS=Momordica charantia... [more]
A0A1S3C5P10.080.92serine/threonine-protein kinase At3g07070 OS=Cucumis melo OX=3656 GN=LOC10349733... [more]
A0A6J1FW590.080.99probable serine/threonine-protein kinase PBL26 isoform X1 OS=Cucurbita moschata ... [more]
A0A5D3BJR40.079.80Serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0LJ740.078.99Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G270... [more]
Match NameE-valueIdentityDescription
AT2G16750.11.5e-15147.12Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT1G77280.11.2e-12437.11Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT4G35030.31.0e-12051.40Protein kinase superfamily protein [more]
AT1G21590.11.3e-12035.90Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT5G63940.12.9e-11536.39Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 329..600
e-value: 2.9E-26
score: 103.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 330..544
e-value: 7.1E-40
score: 137.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 329..610
score: 33.813488
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 291..403
e-value: 6.9E-26
score: 92.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 404..616
e-value: 2.9E-54
score: 185.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 165..189
NoneNo IPR availablePANTHERPTHR27001:SF820PROTEIN KINASE SUPERFAMILY PROTEINcoord: 20..669
NoneNo IPR availablePANTHERPTHR27001OS01G0253100 PROTEINcoord: 20..669
NoneNo IPR availableCDDcd00293USP_Likecoord: 23..147
e-value: 2.41566E-7
score: 48.135
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 21..126
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 4..141
e-value: 2.2E-5
score: 26.5
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 450..462
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 310..600

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0845.1MC02g0845.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity