MC02g0828 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0828
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionULP_PROTEASE domain-containing protein
LocationMC02: 6589128 .. 6590061 (+)
RNA-Seq ExpressionMC02g0828
SyntenyMC02g0828
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGTCCGAAGGAGATAAGAAGATCTTAAGCTACAACGATGTTGTACTCAGAAAATCAGACTTGGACATCCTTAGCGGTCCATATTATCTGAATGATCGCATCATTGAGTTCTATTTTAGCTATCTATCCTCATACCATCCTTCTAAAGACATCCTTCTAGCTCCACCTTCAATTGCTTTCTGGACAATGAACTGCCCCGACTCCAATAGTCTCGACGATTTCCTACGACCTCTCGATCTGTTTAACAAGAAGCTTGTGATCTTCCCCGTCAATGACAACGACGATGTTAGCAAGGCCGAAGGTGGGAACCACTGGAGTCTTCTTGCTTTTTACAGAGAATCAAACACATTCATTCATCATGACAGCAACAGAGGGATGAATAAACATCACGCCAAGAGACTCTACAAAGCTGTGGCTAGATTCACCAGCAACGACTCGAATTTGACGTCTGAGCCCGGATATGTGGAATGCATGGAGTCGCCTCAGCAAGTAAACGGTTATGATTGTGGTTTATATGTTACAGCCATAGCAAGAAGCATATGTAGCTGGTATGCGCAGAGGAAAGAGGTCGATGGAGATGGTGGTTGGTTTTGTGCAGTGGTGGAGGAGGTTACCCCATGTTCTGTTGCTAATATGCGTAGCGAAATCCTGGAGCTAATAAGAAGTTTAATGGCTGTGAAGGAATACCAACCCGCTTCAAGATAATATTGTTGTTAACTTTACTTACGATTTTATATTACTTTTTATGGCCATTTTTGTGTTGTTCATGGAGACTTTGAATTTGGTCGAAATGATCTGACTTGGTCTCGATCGCTCTGGCAGATGAACGGGAGGAAGTCGATATAGCATTTTGGCTATTAGTACTTGATGTTTGGTGTATATACATTTTTTAATTTAGTCACTAATTTTTTAACATTTTACAATTGAG

mRNA sequence

ATGAGGAAGTCCGAAGGAGATAAGAAGATCTTAAGCTACAACGATGTTGTACTCAGAAAATCAGACTTGGACATCCTTAGCGGTCCATATTATCTGAATGATCGCATCATTGAGTTCTATTTTAGCTATCTATCCTCATACCATCCTTCTAAAGACATCCTTCTAGCTCCACCTTCAATTGCTTTCTGGACAATGAACTGCCCCGACTCCAATAGTCTCGACGATTTCCTACGACCTCTCGATCTGTTTAACAAGAAGCTTGTGATCTTCCCCGTCAATGACAACGACGATGTTAGCAAGGCCGAAGGTGGGAACCACTGGAGTCTTCTTGCTTTTTACAGAGAATCAAACACATTCATTCATCATGACAGCAACAGAGGGATGAATAAACATCACGCCAAGAGACTCTACAAAGCTGTGGCTAGATTCACCAGCAACGACTCGAATTTGACGTCTGAGCCCGGATATGTGGAATGCATGGAGTCGCCTCAGCAAGTAAACGGTTATGATTGTGGTTTATATGTTACAGCCATAGCAAGAAGCATATGTAGCTGGTATGCGCAGAGGAAAGAGGTCGATGGAGATGGTGGTTGGTTTTGTGCAGTGGTGGAGGAGGTTACCCCATGTTCTGTTGCTAATATGCGTAGCGAAATCCTGGAGCTAATAAGAAGTTTAATGGCTGTGAAGGAATACCAACCCGCTTCAAGATAATATTGTTGTTAACTTTACTTACGATTTTATATTACTTTTTATGGCCATTTTTGTGTTGTTCATGGAGACTTTGAATTTGGTCGAAATGATCTGACTTGGTCTCGATCGCTCTGGCAGATGAACGGGAGGAAGTCGATATAGCATTTTGGCTATTAGTACTTGATGTTTGGTGTATATACATTTTTTAATTTAGTCACTAATTTTTTAACATTTTACAATTGAG

Coding sequence (CDS)

ATGAGGAAGTCCGAAGGAGATAAGAAGATCTTAAGCTACAACGATGTTGTACTCAGAAAATCAGACTTGGACATCCTTAGCGGTCCATATTATCTGAATGATCGCATCATTGAGTTCTATTTTAGCTATCTATCCTCATACCATCCTTCTAAAGACATCCTTCTAGCTCCACCTTCAATTGCTTTCTGGACAATGAACTGCCCCGACTCCAATAGTCTCGACGATTTCCTACGACCTCTCGATCTGTTTAACAAGAAGCTTGTGATCTTCCCCGTCAATGACAACGACGATGTTAGCAAGGCCGAAGGTGGGAACCACTGGAGTCTTCTTGCTTTTTACAGAGAATCAAACACATTCATTCATCATGACAGCAACAGAGGGATGAATAAACATCACGCCAAGAGACTCTACAAAGCTGTGGCTAGATTCACCAGCAACGACTCGAATTTGACGTCTGAGCCCGGATATGTGGAATGCATGGAGTCGCCTCAGCAAGTAAACGGTTATGATTGTGGTTTATATGTTACAGCCATAGCAAGAAGCATATGTAGCTGGTATGCGCAGAGGAAAGAGGTCGATGGAGATGGTGGTTGGTTTTGTGCAGTGGTGGAGGAGGTTACCCCATGTTCTGTTGCTAATATGCGTAGCGAAATCCTGGAGCTAATAAGAAGTTTAATGGCTGTGAAGGAATACCAACCCGCTTCAAGATAA

Protein sequence

MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSIAFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFIHHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIARSICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR
Homology
BLAST of MC02g0828 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 8.4e-67
Identity = 124/227 (54.63%), Postives = 161/227 (70.93%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M  +  D KILSY DVVLR+SDLDIL+GP +LNDR+IEFY S+LS+ H S  I L PPSI
Sbjct: 1   MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW  NCPD+  L DF++PL+L +K L+I PVNDN +V  AEGG HWSLL +Y+E+NTF+
Sbjct: 61  AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDS  G+N+  AK+L+KAV+ F SN      +  Y EC ++PQQ NGYDCG+++ A AR
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFVSN-----GDASYKECTDTPQQKNGYDCGVFLLATAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMA 228
            IC W++     + D  WF A V+E  P  V ++R EIL LI+ LM+
Sbjct: 181 VICEWFSSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221

BLAST of MC02g0828 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.0e-27
Identity = 74/223 (33.18%), Postives = 121/223 (54.26%), Query Frame = 0

Query: 7   DKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYL--SSYHPSKD-ILLAPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y   S +H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQF 61

Query: 67  TMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFIHHD 126
                +   +  FL PLDL NK++V   +NDN   ++A GG HWSLL + ++ N+F H+D
Sbjct: 62  IKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIARSIC 186
           S+   N  HAK++ + +  F     +   +  +VE  ++P Q N YDCG+YV     ++C
Sbjct: 122 SHSRSNSVHAKQVAEKLEAFLGRKGD---KLAFVE-EKAPAQQNSYDCGMYVICNTEALC 181

Query: 187 -SWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSL 226
            +++ Q+ E         ++++ +TP  +   R E  +LI +L
Sbjct: 182 QNFFRQQTE---------SLLQLLTPAYITKKRGEWKDLITTL 209

BLAST of MC02g0828 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 7.2e-26
Identity = 73/227 (32.16%), Postives = 117/227 (51.54%), Query Frame = 0

Query: 7   DKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYL--SSYHPSKD-ILLAPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y   S +H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQF 61

Query: 67  TMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFIHHD 126
                    +  FL PLDL +K++V   +NDN   ++A GG HWSLL + ++ N+F H+D
Sbjct: 62  IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIARSIC 186
           S+   N  HAK++ + +  F  +  +   +  +VE  ++P Q N YDCG+YV     ++C
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLGSKGD---KLVFVE-EKAPAQENSYDCGMYVICNTEALC 181

Query: 187 SWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKE 231
               +R+          + ++ +TP  +   R E  +LI  L    E
Sbjct: 182 QSLFRRQPE--------SPLQLLTPTYITKKRGEWKDLIARLAKKNE 214

BLAST of MC02g0828 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 7.2e-26
Identity = 72/222 (32.43%), Postives = 117/222 (52.70%), Query Frame = 0

Query: 7   DKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSS--YHPSKD-ILLAPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y +S  +H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQF 61

Query: 67  TMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFIHHD 126
                    +  FL PLDL +K++V   +NDN   ++A GG HWSLL + ++ N+F H+D
Sbjct: 62  IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIARSIC 186
           S+   N  HAK++ + +  F  +  +   +  +VE  ++P Q N YDCG+YV     ++C
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLGSKGD---KLVFVE-EKAPAQQNSYDCGMYVICNTEALC 181

Query: 187 SWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSL 226
               +R+          + ++ +TP  +   R E  +LI  L
Sbjct: 182 QNLFRRQPE--------SPLQLLTPTYITKKRGEWKDLIARL 209

BLAST of MC02g0828 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.6e-23
Identity = 73/239 (30.54%), Postives = 111/239 (46.44%), Query Frame = 0

Query: 7   DKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYL------SSYHPSKDILLAPPSI 66
           D  I+SYND  + +SD++IL    +LND II FY  +L      +       +LL  PS+
Sbjct: 3   DPLIVSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLSPSV 62

Query: 67  AFWTMNCPDSNSLD-DFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTF 126
            F          +   F +PL L  K+++ FP+ +N D +   GG HWSLL F +  N F
Sbjct: 63  VFCCSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSLNKF 122

Query: 127 IHHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIA 186
           I++DS    N   A  +        S+    T+   ++   ++PQQ NGYDCGLYV +I 
Sbjct: 123 IYYDSINSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVLSII 182

Query: 187 RSICSWYAQRKEVD-GDGGWFC------------AVVEEVTPCSVANMRSEILELIRSL 226
             +     +  E + G+                  + +E+TP  + + R EIL  I  L
Sbjct: 183 EELLKLIIKENENNKGEENKISYKDLLLSEFTNELLFKEITPNYIKDKRVEILNTILKL 241

BLAST of MC02g0828 vs. NCBI nr
Match: XP_022146090.1 (NEDD8-specific protease 1 [Momordica charantia])

HSP 1 Score: 492 bits (1267), Expect = 7.42e-176
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI
Sbjct: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI
Sbjct: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR
Sbjct: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR
Sbjct: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236

BLAST of MC02g0828 vs. NCBI nr
Match: XP_038902669.1 (NEDD8-specific protease 1 [Benincasa hispida])

HSP 1 Score: 404 bits (1037), Expect = 8.55e-141
Identity = 192/236 (81.36%), Postives = 213/236 (90.25%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDIL GPYYLNDRI EFYFSYLS+ HPSKDILL+PPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILGGPYYLNDRIPEFYFSYLSTTHPSKDILLSPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW MNCPD+ SLDDFL+PL+L +KKLVIFPVNDN+DVSKAEGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWIMNCPDAESLDDFLKPLNLCDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDS++GMNK+HAKRLY+AVARF S DSN TS P Y+EC+ESPQQVNGYDCG+YV AIAR
Sbjct: 121 HHDSHKGMNKYHAKRLYRAVARFMSADSNSTSNPSYLECVESPQQVNGYDCGVYVIAIAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           +IC WY QRKEVD DG WF AVV+EV+P  V NMRSEIL LIRSLMAVKEYQPAS+
Sbjct: 181 TICRWYEQRKEVDRDGLWFSAVVKEVSPSFVTNMRSEILGLIRSLMAVKEYQPASK 236

BLAST of MC02g0828 vs. NCBI nr
Match: XP_008463889.1 (PREDICTED: NEDD8-specific protease 1 [Cucumis melo] >KAA0035294.1 NEDD8-specific protease 1 [Cucumis melo var. makuwa] >TYK14342.1 NEDD8-specific protease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 389 bits (998), Expect = 7.48e-135
Identity = 184/236 (77.97%), Postives = 209/236 (88.56%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M K EGD+KILS+NDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLS+ HPS DILL+PPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW MNCPD  SL+ FL PL+L +KKLVIFPVNDN DVSK EGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDSN+GMNK+ AKRLYKAVARF ++ SN TS P Y+EC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SIC WY +R+EV+ DG WF AVVEE++P  VANMR EILELIRSLMAVKEYQPA++
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

BLAST of MC02g0828 vs. NCBI nr
Match: XP_004149559.1 (NEDD8-specific protease 1 [Cucumis sativus] >XP_031742683.1 NEDD8-specific protease 1 [Cucumis sativus] >XP_031742684.1 NEDD8-specific protease 1 [Cucumis sativus] >KGN47289.1 hypothetical protein Csa_022955 [Cucumis sativus])

HSP 1 Score: 379 bits (973), Expect = 4.81e-131
Identity = 182/236 (77.12%), Postives = 205/236 (86.86%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLS+ HPS  ILL+PPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNGILLSPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW MNCPD  SL+ FL PL+L +KKLVIFPVNDN DVSK EGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWMMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDSN+GMNK+ AKRLY AVARF ++ S  T  P YVEC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYAAKRLYNAVARFMNDGSISTLNPSYVECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SIC WY +R+EV+ DG WF AVVEE++P  VANMR EIL LIRSLMAVKEYQPA++
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPSLVANMRREILGLIRSLMAVKEYQPATK 236

BLAST of MC02g0828 vs. NCBI nr
Match: XP_023532288.1 (NEDD8-specific protease 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362 bits (929), Expect = 2.59e-124
Identity = 180/239 (75.31%), Postives = 204/239 (85.36%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDIL+GP YLNDRIIEFYFSYL + HPS+DILL+PPSI
Sbjct: 1   MSKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW MNCPD++ L DFL+PL+L N+ LVIFPVNDN+DVSKAEGGNHWS+L FYRE+N F+
Sbjct: 61  AFWIMNCPDTDGLKDFLQPLNLLNRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDS +G+N   AKRLY+AVARF    +N TSEP YVEC ESPQQVNG+DCGLYVTAI+R
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFMGG-TNSTSEPSYVECSESPQQVNGHDCGLYVTAISR 180

Query: 181 SICSWYAQ-RKEVD-GDGG-WFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SIC WY Q RKEV  GDG  WF AV +EVTP  VA+MRSEIL LIRS MA+KEYQPAS+
Sbjct: 181 SICRWYEQERKEVGCGDGFLWFSAVAKEVTPSLVADMRSEILGLIRSFMALKEYQPASK 238

BLAST of MC02g0828 vs. ExPASy TrEMBL
Match: A0A6J1CWB2 (NEDD8-specific protease 1 OS=Momordica charantia OX=3673 GN=LOC111015386 PE=3 SV=1)

HSP 1 Score: 492 bits (1267), Expect = 3.59e-176
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI
Sbjct: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI
Sbjct: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR
Sbjct: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR
Sbjct: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236

BLAST of MC02g0828 vs. ExPASy TrEMBL
Match: A0A5D3CSG4 (NEDD8-specific protease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00810 PE=3 SV=1)

HSP 1 Score: 389 bits (998), Expect = 3.62e-135
Identity = 184/236 (77.97%), Postives = 209/236 (88.56%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M K EGD+KILS+NDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLS+ HPS DILL+PPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW MNCPD  SL+ FL PL+L +KKLVIFPVNDN DVSK EGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDSN+GMNK+ AKRLYKAVARF ++ SN TS P Y+EC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SIC WY +R+EV+ DG WF AVVEE++P  VANMR EILELIRSLMAVKEYQPA++
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

BLAST of MC02g0828 vs. ExPASy TrEMBL
Match: A0A1S3CK81 (NEDD8-specific protease 1 OS=Cucumis melo OX=3656 GN=LOC103501907 PE=3 SV=1)

HSP 1 Score: 389 bits (998), Expect = 3.62e-135
Identity = 184/236 (77.97%), Postives = 209/236 (88.56%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M K EGD+KILS+NDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLS+ HPS DILL+PPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW MNCPD  SL+ FL PL+L +KKLVIFPVNDN DVSK EGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDSN+GMNK+ AKRLYKAVARF ++ SN TS P Y+EC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SIC WY +R+EV+ DG WF AVVEE++P  VANMR EILELIRSLMAVKEYQPA++
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

BLAST of MC02g0828 vs. ExPASy TrEMBL
Match: A0A0A0KEM9 (ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G289720 PE=3 SV=1)

HSP 1 Score: 379 bits (973), Expect = 2.33e-131
Identity = 182/236 (77.12%), Postives = 205/236 (86.86%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLS+ HPS  ILL+PPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNGILLSPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW MNCPD  SL+ FL PL+L +KKLVIFPVNDN DVSK EGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWMMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDSN+GMNK+ AKRLY AVARF ++ S  T  P YVEC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYAAKRLYNAVARFMNDGSISTLNPSYVECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SIC WY +R+EV+ DG WF AVVEE++P  VANMR EIL LIRSLMAVKEYQPA++
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPSLVANMRREILGLIRSLMAVKEYQPATK 236

BLAST of MC02g0828 vs. ExPASy TrEMBL
Match: A0A6J1KYI9 (NEDD8-specific protease 1 OS=Cucurbita maxima OX=3661 GN=LOC111499890 PE=3 SV=1)

HSP 1 Score: 361 bits (927), Expect = 2.53e-124
Identity = 179/239 (74.90%), Postives = 204/239 (85.36%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDIL+GP YLNDRIIEFYFSYL + HPS+DILL+PPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW MNCPD++ L+DFL+PL+L  + LVIFPVNDN+DVSKAEGGNHWS+L FYRE+N F+
Sbjct: 61  AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDS +G+N   AKRLY+AVARF    +N TSEP YVEC ESPQQVNG+DCGLYVTAI+R
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFMGV-TNSTSEPSYVECSESPQQVNGHDCGLYVTAISR 180

Query: 181 SICSWYAQ-RKEVDGDGG--WFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236
           SIC WY Q RKE  G  G  WF AVV+EVTP SVA+MRSEIL LIRS MA+KEYQPAS+
Sbjct: 181 SICRWYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMALKEYQPASK 238

BLAST of MC02g0828 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 6.0e-68
Identity = 124/227 (54.63%), Postives = 161/227 (70.93%), Query Frame = 0

Query: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60
           M  +  D KILSY DVVLR+SDLDIL+GP +LNDR+IEFY S+LS+ H S  I L PPSI
Sbjct: 1   MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60

Query: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120
           AFW  NCPD+  L DF++PL+L +K L+I PVNDN +V  AEGG HWSLL +Y+E+NTF+
Sbjct: 61  AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120

Query: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180
           HHDS  G+N+  AK+L+KAV+ F SN      +  Y EC ++PQQ NGYDCG+++ A AR
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFVSN-----GDASYKECTDTPQQKNGYDCGVFLLATAR 180

Query: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMA 228
            IC W++     + D  WF A V+E  P  V ++R EIL LI+ LM+
Sbjct: 181 VICEWFSSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221

BLAST of MC02g0828 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 2.9e-06
Identity = 49/210 (23.33%), Postives = 80/210 (38.10%), Query Frame = 0

Query: 16  VVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSIAFWTMNC-------- 75
           VV+RK D+++L    ++ND II+FY  YL +    K+         F   NC        
Sbjct: 305 VVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKE------RGRFHFFNCFFFRKLAN 364

Query: 76  -----PDS-------NSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRE 135
                P +         +  + + +DLF K  +  P+N +          HWSL+     
Sbjct: 365 LDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCS---------FHWSLVIICHP 424

Query: 136 SN----------------------TFIHHDSNRGMNK--------HHAKRLYKAVARFTS 176
                                     +H DS +G +K         + +  +KA    T+
Sbjct: 425 GELVPSHVNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTT 484

BLAST of MC02g0828 vs. TAIR 10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.1e-04
Identity = 47/204 (23.04%), Postives = 78/204 (38.24%), Query Frame = 0

Query: 16  VVLRKSDLDILSGPYYLNDRIIEFYFSYLSS-----------------YHPSKDILLAPP 75
           V + K D+++L    ++ND II+FY +YL +                 +    D+   P 
Sbjct: 426 VSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPS 485

Query: 76  SIAFWTMNCPDSNS----LDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYR 135
           SIA       D  +    +  + R +D+F K  +  PVN N          HWSL+    
Sbjct: 486 SIA-------DGKAAFLRVRKWTRKVDMFGKDYIFVPVNYN---------LHWSLIVICH 545

Query: 136 ESNT----------------FIHHDSNRGMN-------KHHAKRLYKAVARFTSNDSNLT 176
                                +H DS +G +       + +    +K   + TS+D +  
Sbjct: 546 PGEVANRTDLDLDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSR 605

BLAST of MC02g0828 vs. TAIR 10
Match: AT1G09730.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.1e-04
Identity = 47/204 (23.04%), Postives = 78/204 (38.24%), Query Frame = 0

Query: 16  VVLRKSDLDILSGPYYLNDRIIEFYFSYLSS-----------------YHPSKDILLAPP 75
           V + K D+++L    ++ND II+FY +YL +                 +    D+   P 
Sbjct: 394 VSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPS 453

Query: 76  SIAFWTMNCPDSNS----LDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYR 135
           SIA       D  +    +  + R +D+F K  +  PVN N          HWSL+    
Sbjct: 454 SIA-------DGKAAFLRVRKWTRKVDMFGKDYIFVPVNYN---------LHWSLIVICH 513

Query: 136 ESNT----------------FIHHDSNRGMN-------KHHAKRLYKAVARFTSNDSNLT 176
                                +H DS +G +       + +    +K   + TS+D +  
Sbjct: 514 PGEVANRTDLDLDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSR 573

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LSS78.4e-6754.63NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1[more]
Q96LD81.0e-2733.18Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1[more]
Q9D2Z47.2e-2632.16Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2[more]
Q5FVJ87.2e-2632.43Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1[more]
Q54XR22.6e-2330.54Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen... [more]
Match NameE-valueIdentityDescription
XP_022146090.17.42e-176100.00NEDD8-specific protease 1 [Momordica charantia][more]
XP_038902669.18.55e-14181.36NEDD8-specific protease 1 [Benincasa hispida][more]
XP_008463889.17.48e-13577.97PREDICTED: NEDD8-specific protease 1 [Cucumis melo] >KAA0035294.1 NEDD8-specific... [more]
XP_004149559.14.81e-13177.12NEDD8-specific protease 1 [Cucumis sativus] >XP_031742683.1 NEDD8-specific prote... [more]
XP_023532288.12.59e-12475.31NEDD8-specific protease 1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1CWB23.59e-176100.00NEDD8-specific protease 1 OS=Momordica charantia OX=3673 GN=LOC111015386 PE=3 SV... [more]
A0A5D3CSG43.62e-13577.97NEDD8-specific protease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CK813.62e-13577.97NEDD8-specific protease 1 OS=Cucumis melo OX=3656 GN=LOC103501907 PE=3 SV=1[more]
A0A0A0KEM92.33e-13177.12ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G28972... [more]
A0A6J1KYI92.53e-12474.90NEDD8-specific protease 1 OS=Cucurbita maxima OX=3661 GN=LOC111499890 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G60190.16.0e-6854.63Cysteine proteinases superfamily protein [more]
AT4G33620.12.9e-0623.33Cysteine proteinases superfamily protein [more]
AT1G09730.12.1e-0423.04Cysteine proteinases superfamily protein [more]
AT1G09730.22.1e-0423.04Cysteine proteinases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 7..228
e-value: 3.8E-64
score: 218.4
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 31..180
e-value: 2.3E-15
score: 57.1
IPR003653Ulp1 protease family, C-terminal catalytic domainPROSITEPS50600ULP_PROTEASEcoord: 16..182
score: 15.258015
IPR044613NEDD8-specific protease 1/2-likePANTHERPTHR46468SENTRIN-SPECIFIC PROTEASE 8coord: 3..226
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 7..226

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0828.1MC02g0828.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0019784 NEDD8-specific protease activity
molecular_function GO:0008234 cysteine-type peptidase activity