MC02g0731 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0731
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description50S ribosomal protein L4
LocationMC02: 5845039 .. 5845872 (+)
RNA-Seq ExpressionMC02g0731
SyntenyMC02g0731
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACCTCCACGACGCCATCATCGCTCTCTTTCTTCACTTCTTCGGTGTTTCTTTCCTCTGCGAACCAAAATTCGAAGCTGTTTTGCCATTGGAAACCTAATTCCCTAAAACCCCAAACCAAGCCCTTATCGATCTCCTCACAGCTCGCTACTCTTCCGGTCCTCTCCTTCGCCGGAGAAAAAGTCGGCGAGACCTACCTCGACCTGAAATCTGCTCCACCGGAAACCGCCCGCGCCGTTGTTCACCGAGCCATTATCACCGATCAACAGAACAAACGCCGTGGGACCGCCTCGACTCTCACCAGAGCTGAGGTTAGCGGAGGAGGCAAAAAACCCTACCAGCAGAAGAAGACGGGGAAGGCCCGCCGCGGTTCGATGCGTACTCCTCTCCGGCCTGGTGGGGGCGTGGTCTTCGGGCCCAAACCTAGAGATTGGTCCATTAAAATCAACAGAAAGGAGAAGAGATTGGCGATATCGACGGCGGTGGCTAGCGCGGCAGTGAATACGATTGTGGTGGAAGATTTTGGCGACAAATTCGAGAAGCCGAAAACGAAAGAGTTCATTGCTGCACTGAAGAGGTGGGGAATCGACCCAAAGGAGAAATCGTTGTTCTTAATGACGGAGGTTTCAGATAATGTGCGACTTTCCAGCCGCAATATCGGAACCTTGAAATTGTTGACGCCTAGGACACTGAATTTGTTCGATATCTTGGATTCCGATAAGATGGTTTTGACGCCGGCAGCGGTGGATTACCTGAACGAGAGGTATGGAATCAATTACGAAGGAGGAATCGAAGACGACGAGGAAGAAGAAGAAGAAGAACAACAAGGT

mRNA sequence

ATGGCGACCTCCACGACGCCATCATCGCTCTCTTTCTTCACTTCTTCGGTGTTTCTTTCCTCTGCGAACCAAAATTCGAAGCTGTTTTGCCATTGGAAACCTAATTCCCTAAAACCCCAAACCAAGCCCTTATCGATCTCCTCACAGCTCGCTACTCTTCCGGTCCTCTCCTTCGCCGGAGAAAAAGTCGGCGAGACCTACCTCGACCTGAAATCTGCTCCACCGGAAACCGCCCGCGCCGTTGTTCACCGAGCCATTATCACCGATCAACAGAACAAACGCCGTGGGACCGCCTCGACTCTCACCAGAGCTGAGGTTAGCGGAGGAGGCAAAAAACCCTACCAGCAGAAGAAGACGGGGAAGGCCCGCCGCGGTTCGATGCGTACTCCTCTCCGGCCTGGTGGGGGCGTGGTCTTCGGGCCCAAACCTAGAGATTGGTCCATTAAAATCAACAGAAAGGAGAAGAGATTGGCGATATCGACGGCGGTGGCTAGCGCGGCAGTGAATACGATTGTGGTGGAAGATTTTGGCGACAAATTCGAGAAGCCGAAAACGAAAGAGTTCATTGCTGCACTGAAGAGGTGGGGAATCGACCCAAAGGAGAAATCGTTGTTCTTAATGACGGAGGTTTCAGATAATGTGCGACTTTCCAGCCGCAATATCGGAACCTTGAAATTGTTGACGCCTAGGACACTGAATTTGTTCGATATCTTGGATTCCGATAAGATGGTTTTGACGCCGGCAGCGGTGGATTACCTGAACGAGAGGTATGGAATCAATTACGAAGGAGGAATCGAAGACGACGAGGAAGAAGAAGAAGAAGAACAACAAGGT

Coding sequence (CDS)

ATGGCGACCTCCACGACGCCATCATCGCTCTCTTTCTTCACTTCTTCGGTGTTTCTTTCCTCTGCGAACCAAAATTCGAAGCTGTTTTGCCATTGGAAACCTAATTCCCTAAAACCCCAAACCAAGCCCTTATCGATCTCCTCACAGCTCGCTACTCTTCCGGTCCTCTCCTTCGCCGGAGAAAAAGTCGGCGAGACCTACCTCGACCTGAAATCTGCTCCACCGGAAACCGCCCGCGCCGTTGTTCACCGAGCCATTATCACCGATCAACAGAACAAACGCCGTGGGACCGCCTCGACTCTCACCAGAGCTGAGGTTAGCGGAGGAGGCAAAAAACCCTACCAGCAGAAGAAGACGGGGAAGGCCCGCCGCGGTTCGATGCGTACTCCTCTCCGGCCTGGTGGGGGCGTGGTCTTCGGGCCCAAACCTAGAGATTGGTCCATTAAAATCAACAGAAAGGAGAAGAGATTGGCGATATCGACGGCGGTGGCTAGCGCGGCAGTGAATACGATTGTGGTGGAAGATTTTGGCGACAAATTCGAGAAGCCGAAAACGAAAGAGTTCATTGCTGCACTGAAGAGGTGGGGAATCGACCCAAAGGAGAAATCGTTGTTCTTAATGACGGAGGTTTCAGATAATGTGCGACTTTCCAGCCGCAATATCGGAACCTTGAAATTGTTGACGCCTAGGACACTGAATTTGTTCGATATCTTGGATTCCGATAAGATGGTTTTGACGCCGGCAGCGGTGGATTACCTGAACGAGAGGTATGGAATCAATTACGAAGGAGGAATCGAAGACGACGAGGAAGAAGAAGAAGAAGAACAACAAGGT

Protein sequence

MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAGEKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTGKARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDSDKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG
Homology
BLAST of MC02g0731 vs. ExPASy Swiss-Prot
Match: O50061 (50S ribosomal protein L4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL4 PE=2 SV=2)

HSP 1 Score: 365.5 bits (937), Expect = 5.2e-100
Identity = 191/279 (68.46%), Postives = 235/279 (84.23%), Query Frame = 0

Query: 2   ATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAGE 61
           +++T P+SLSFF+SS+FLSS++Q  K +           +KPLS+SSQLATLP+LSF GE
Sbjct: 3   SSATAPNSLSFFSSSLFLSSSHQIPKTYISVSKLGSGRVSKPLSVSSQLATLPILSFEGE 62

Query: 62  KVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTGK 121
           KVGETYLDLK+AP +TARAVVHRAI+TD  NKRRGTASTLTR EV GGG KPY QKKTG 
Sbjct: 63  KVGETYLDLKAAPEDTARAVVHRAIVTDLNNKRRGTASTLTRGEVRGGGIKPYSQKKTGH 122

Query: 122 ARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAV---NTIVVEDFGD 181
           ARRGS RTPLRPGGGVVFGP+P+DWSIKINRKEK+LAISTA++SAA      IVVE+FG+
Sbjct: 123 ARRGSQRTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGE 182

Query: 182 KFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL 241
           KFEKPKTK+F+AA++RWG+DPKEK++FLM +V +NV  SSRNIGTL++LTPRTLNLFDIL
Sbjct: 183 KFEKPKTKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDIL 242

Query: 242 DSDKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQ 278
           ++DK+VLTPAAV++LN RYG++     +DDE+E E  ++
Sbjct: 243 NADKLVLTPAAVEFLNARYGVDAVEEEDDDEDETEGSEE 281

BLAST of MC02g0731 vs. ExPASy Swiss-Prot
Match: O80361 (50S ribosomal protein L4, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL4 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.3e-95
Identity = 189/272 (69.49%), Postives = 228/272 (83.82%), Query Frame = 0

Query: 8   SSLSFFTSSVFLSSAN-QNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAGEKVGET 67
           +SLSFF+SS+FLS+ N Q+SK      P  L       +I S++AT+P+LSF G +VG T
Sbjct: 3   ASLSFFSSSIFLSNPNIQSSKHLLFRSPKQLS-VAAIATIRSEVATIPILSFDGAEVGST 62

Query: 68  YLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTGKARRGS 127
            L+LKSAP +TARAVVHR + TD +N+RRGTASTLTR+EV GGG KPY QKKTG+ARRGS
Sbjct: 63  TLNLKSAPLDTARAVVHRGLTTDLRNQRRGTASTLTRSEVRGGGIKPYPQKKTGRARRGS 122

Query: 128 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 187
            RTPLRPGGGVVFGPKP+DWS+KIN+KEKRLAISTA++SA+ NTIVVE+F DKFEKPKTK
Sbjct: 123 NRTPLRPGGGVVFGPKPKDWSVKINKKEKRLAISTALSSASENTIVVEEFNDKFEKPKTK 182

Query: 188 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDSDKMVLT 247
           EFI  ++RWG+DPKEKSLFLMT+VSDNV LSSRNIGTLK+LTPRTLNLFDILDS+K+VLT
Sbjct: 183 EFIDLMRRWGLDPKEKSLFLMTDVSDNVILSSRNIGTLKMLTPRTLNLFDILDSEKLVLT 242

Query: 248 PAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 279
            +AVD+LNERYG   E   ED+EE+++E+  G
Sbjct: 243 KSAVDFLNERYGDENEW--EDEEEDDQEDNDG 271

BLAST of MC02g0731 vs. ExPASy Swiss-Prot
Match: O49937 (50S ribosomal protein L4, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL4 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.0e-91
Identity = 178/280 (63.57%), Postives = 228/280 (81.43%), Query Frame = 0

Query: 1   MATSTTPS-SLSFFTSSVFLS-SANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSF 60
           MATST+ S SLSFF+SS+F S S N +SK       +S    +  LSI S+L  LP+L+F
Sbjct: 1   MATSTSSSLSLSFFSSSLFSSKSRNFSSKPILKLPSSSHSQTSLSLSIKSELIPLPILNF 60

Query: 61  AGEKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKK 120
           +GEKVGET+L+LK+APPE ARAVVHR +IT  QNKRRGTASTLTRAEV GGG+KPY QKK
Sbjct: 61  SGEKVGETFLNLKTAPPEKARAVVHRGLITHLQNKRRGTASTLTRAEVRGGGRKPYPQKK 120

Query: 121 TGKARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGD 180
           TG+ARRGS  +PLRPGGGV+FGPKPRDW+IK+N+KE+RLA+STA+ASA  N+ VVE+F +
Sbjct: 121 TGRARRGSQGSPLRPGGGVIFGPKPRDWTIKMNKKERRLALSTAIASAVGNSFVVEEFAE 180

Query: 181 KFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL 240
            FEKPKTK+FIAA++RWG+DP EKSLF + ++ +NV  S RNI TLKLLTPR+LNLFD+L
Sbjct: 181 NFEKPKTKDFIAAMQRWGLDPAEKSLFFLMDLVENVEKSGRNIRTLKLLTPRSLNLFDVL 240

Query: 241 DSDKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 279
           +++K+V T   + YLN+RYG++    +ED++EEEEEE++G
Sbjct: 241 NAEKLVFTEGTIQYLNQRYGVD---TLEDEDEEEEEEEEG 277

BLAST of MC02g0731 vs. ExPASy Swiss-Prot
Match: Q8DMN0 (50S ribosomal protein L4 OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=rplD PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.3e-55
Identity = 109/204 (53.43%), Postives = 148/204 (72.55%), Query Frame = 0

Query: 55  VLSFAGEKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPY 114
           V  + G   GE  LDL +A PETA  +VHRA++    N R+GT ST TRAEV GGG+KP+
Sbjct: 6   VKDWQGADSGEATLDLATAKPETASHIVHRALVRQLANARQGTVSTKTRAEVRGGGRKPW 65

Query: 115 QQKKTGKARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVE 174
           +QK TG+AR GS+R+PL  GGGV+FGPKPRD+S K+NRKE+RLA+ TA+ S   + IVV+
Sbjct: 66  RQKGTGRARAGSIRSPLWRGGGVIFGPKPRDYSQKMNRKERRLALRTALMSRVEDLIVVQ 125

Query: 175 DFGDKFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNL 234
           DF D   +PKTKE + AL+RWGI+P++K L L  ++++ V LS+RN+ TLKLL    LN+
Sbjct: 126 DFADHLPRPKTKELVTALQRWGIEPEQKVLLLAADITETVALSARNVPTLKLLRADQLNV 185

Query: 235 FDILDSDKMVLTPAAVDYLNERYG 259
           FD+L +D++V T  A+  + E YG
Sbjct: 186 FDLLYADRIVATTGAIAKIQEVYG 209

BLAST of MC02g0731 vs. ExPASy Swiss-Prot
Match: B0JI02 (50S ribosomal protein L4 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=rplD PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.9e-55
Identity = 105/204 (51.47%), Postives = 152/204 (74.51%), Query Frame = 0

Query: 55  VLSFAGEKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPY 114
           V ++ GE+ G   L+LK+A PETA+ ++HR +++     R+G AS+ TR+EV GGG+KP+
Sbjct: 6   VKNWQGEEAGTGTLELKTAKPETAKHLIHRVVVSHLAAARQGNASSKTRSEVRGGGRKPW 65

Query: 115 QQKKTGKARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVE 174
           +QK TG+AR GS+R+PL  GGGV+FGPKPRD+ +K+NRKEKRLA+ TA+ S A N IVVE
Sbjct: 66  RQKGTGRARAGSIRSPLWRGGGVIFGPKPRDFEVKVNRKEKRLALRTALISQADNFIVVE 125

Query: 175 DFGDKFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNL 234
            F ++F +PKTKE  AAL RWG  P+EK L ++TE+ +NV LS RNI  LK++   +LN+
Sbjct: 126 SFAEQFSQPKTKELTAALSRWGASPEEKILLILTEIPENVYLSGRNICNLKIIRADSLNV 185

Query: 235 FDILDSDKMVLTPAAVDYLNERYG 259
           +D++ +D+++ T AA+  + E YG
Sbjct: 186 YDVILADRVIATAAALAKIEEVYG 209

BLAST of MC02g0731 vs. NCBI nr
Match: XP_022148353.1 (50S ribosomal protein L4, chloroplastic [Momordica charantia])

HSP 1 Score: 531 bits (1369), Expect = 1.00e-189
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG
Sbjct: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG
Sbjct: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278
           DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG
Sbjct: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278

BLAST of MC02g0731 vs. NCBI nr
Match: XP_023532270.1 (50S ribosomal protein L4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 504 bits (1299), Expect = 4.18e-179
Identity = 263/278 (94.60%), Postives = 272/278 (97.84%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MA STTPSSLSFF+SS+FLSSANQNSKLFC+WKPNS KPQ+KPLSISSQLATLPVLSF G
Sbjct: 1   MAASTTPSSLSFFSSSIFLSSANQNSKLFCNWKPNSFKPQSKPLSISSQLATLPVLSFTG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGET LDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGG+KPYQQKKTG
Sbjct: 61  EKVGETVLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGRKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL+S
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278
           DK+VLTPAAVDYLNERYGINYE GIED+EEEEEEE +G
Sbjct: 241 DKLVLTPAAVDYLNERYGINYEEGIEDEEEEEEEELEG 278

BLAST of MC02g0731 vs. NCBI nr
Match: XP_022948154.1 (50S ribosomal protein L4, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6605425.1 50S ribosomal protein L4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035374.1 50S ribosomal protein L4, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 503 bits (1294), Expect = 2.42e-178
Identity = 262/278 (94.24%), Postives = 271/278 (97.48%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MA ST PSSLSFF+SS+FLSSANQNSKLFC+WKPNS KPQ+KPLSISSQLATLPVLSF G
Sbjct: 1   MAASTAPSSLSFFSSSIFLSSANQNSKLFCNWKPNSFKPQSKPLSISSQLATLPVLSFTG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGET LDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGG+KPYQQKKTG
Sbjct: 61  EKVGETVLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGRKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL+S
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278
           DK+VLTPAAVDYLNERYGINYE GIED+EEEEEEE +G
Sbjct: 241 DKLVLTPAAVDYLNERYGINYEEGIEDEEEEEEEELEG 278

BLAST of MC02g0731 vs. NCBI nr
Match: XP_022948155.1 (50S ribosomal protein L4, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 503 bits (1294), Expect = 2.42e-178
Identity = 262/278 (94.24%), Postives = 271/278 (97.48%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MA ST PSSLSFF+SS+FLSSANQNSKLFC+WKPNS KPQ+KPLSISSQLATLPVLSF G
Sbjct: 1   MAASTAPSSLSFFSSSIFLSSANQNSKLFCNWKPNSFKPQSKPLSISSQLATLPVLSFTG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGET LDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGG+KPYQQKKTG
Sbjct: 61  EKVGETVLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGRKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL+S
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278
           DK+VLTPAAVDYLNERYGINYE GIED+EEEEEEE +G
Sbjct: 241 DKLVLTPAAVDYLNERYGINYEEGIEDEEEEEEEELEG 278

BLAST of MC02g0731 vs. NCBI nr
Match: XP_023007555.1 (50S ribosomal protein L4, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 500 bits (1288), Expect = 1.91e-177
Identity = 260/274 (94.89%), Postives = 268/274 (97.81%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MA STTPSSLSFF+SS+FLSSANQNSKLFC+WKPNS KPQ+KPLSISSQLATLPVLSF G
Sbjct: 1   MAASTTPSSLSFFSSSIFLSSANQNSKLFCNWKPNSFKPQSKPLSISSQLATLPVLSFTG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGET LDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEV GGG+KPYQQKKTG
Sbjct: 61  EKVGETVLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVRGGGRKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL+S
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEE 274
           DK+VLTPAAVDYLNERYGINYE GIED+EEEEEE
Sbjct: 241 DKLVLTPAAVDYLNERYGINYEEGIEDEEEEEEE 274

BLAST of MC02g0731 vs. ExPASy TrEMBL
Match: A0A6J1D3V7 (50S ribosomal protein L4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111017027 PE=3 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 4.86e-190
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG
Sbjct: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG
Sbjct: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278
           DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG
Sbjct: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278

BLAST of MC02g0731 vs. ExPASy TrEMBL
Match: A0A6J1G8Y3 (50S ribosomal protein L4, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451819 PE=3 SV=1)

HSP 1 Score: 503 bits (1294), Expect = 1.17e-178
Identity = 262/278 (94.24%), Postives = 271/278 (97.48%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MA ST PSSLSFF+SS+FLSSANQNSKLFC+WKPNS KPQ+KPLSISSQLATLPVLSF G
Sbjct: 1   MAASTAPSSLSFFSSSIFLSSANQNSKLFCNWKPNSFKPQSKPLSISSQLATLPVLSFTG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGET LDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGG+KPYQQKKTG
Sbjct: 61  EKVGETVLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGRKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL+S
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278
           DK+VLTPAAVDYLNERYGINYE GIED+EEEEEEE +G
Sbjct: 241 DKLVLTPAAVDYLNERYGINYEEGIEDEEEEEEEELEG 278

BLAST of MC02g0731 vs. ExPASy TrEMBL
Match: A0A6J1G917 (50S ribosomal protein L4, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451819 PE=3 SV=1)

HSP 1 Score: 503 bits (1294), Expect = 1.17e-178
Identity = 262/278 (94.24%), Postives = 271/278 (97.48%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MA ST PSSLSFF+SS+FLSSANQNSKLFC+WKPNS KPQ+KPLSISSQLATLPVLSF G
Sbjct: 1   MAASTAPSSLSFFSSSIFLSSANQNSKLFCNWKPNSFKPQSKPLSISSQLATLPVLSFTG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGET LDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGG+KPYQQKKTG
Sbjct: 61  EKVGETVLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGRKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL+S
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQG 278
           DK+VLTPAAVDYLNERYGINYE GIED+EEEEEEE +G
Sbjct: 241 DKLVLTPAAVDYLNERYGINYEEGIEDEEEEEEEELEG 278

BLAST of MC02g0731 vs. ExPASy TrEMBL
Match: A0A6J1KZ16 (50S ribosomal protein L4, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500015 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 9.27e-178
Identity = 260/274 (94.89%), Postives = 268/274 (97.81%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MA STTPSSLSFF+SS+FLSSANQNSKLFC+WKPNS KPQ+KPLSISSQLATLPVLSF G
Sbjct: 1   MAASTTPSSLSFFSSSIFLSSANQNSKLFCNWKPNSFKPQSKPLSISSQLATLPVLSFTG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGET LDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEV GGG+KPYQQKKTG
Sbjct: 61  EKVGETVLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVRGGGRKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL+S
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEE 274
           DK+VLTPAAVDYLNERYGINYE GIED+EEEEEE
Sbjct: 241 DKLVLTPAAVDYLNERYGINYEEGIEDEEEEEEE 274

BLAST of MC02g0731 vs. ExPASy TrEMBL
Match: A0A6J1L3A5 (50S ribosomal protein L4, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500015 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 9.27e-178
Identity = 260/274 (94.89%), Postives = 268/274 (97.81%), Query Frame = 0

Query: 1   MATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAG 60
           MA STTPSSLSFF+SS+FLSSANQNSKLFC+WKPNS KPQ+KPLSISSQLATLPVLSF G
Sbjct: 1   MAASTTPSSLSFFSSSIFLSSANQNSKLFCNWKPNSFKPQSKPLSISSQLATLPVLSFTG 60

Query: 61  EKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTG 120
           EKVGET LDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEV GGG+KPYQQKKTG
Sbjct: 61  EKVGETVLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVRGGGRKPYQQKKTG 120

Query: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180
           KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF
Sbjct: 121 KARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKF 180

Query: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDS 240
           EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL+S
Sbjct: 181 EKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNS 240

Query: 241 DKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEE 274
           DK+VLTPAAVDYLNERYGINYE GIED+EEEEEE
Sbjct: 241 DKLVLTPAAVDYLNERYGINYEEGIEDEEEEEEE 274

BLAST of MC02g0731 vs. TAIR 10
Match: AT1G07320.2 (ribosomal protein L4 )

HSP 1 Score: 369.4 bits (947), Expect = 2.6e-102
Identity = 193/277 (69.68%), Postives = 235/277 (84.84%), Query Frame = 0

Query: 2   ATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAGE 61
           +++T P+SLSFF+SS+FLSS++Q  K +           +KPLS+SSQLATLP+LSF GE
Sbjct: 3   SSATAPNSLSFFSSSLFLSSSHQIPKTYISVSKLGSGRVSKPLSVSSQLATLPILSFEGE 62

Query: 62  KVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTGK 121
           KVGETYLDLK+AP +TARAVVHRAI+TD  NKRRGTASTLTR EV GGG KPY QKKTG 
Sbjct: 63  KVGETYLDLKAAPEDTARAVVHRAIVTDLNNKRRGTASTLTRGEVRGGGIKPYSQKKTGH 122

Query: 122 ARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAV---NTIVVEDFGD 181
           ARRGS RTPLRPGGGVVFGP+P+DWSIKINRKEK+LAISTA++SAA      IVVE+FG+
Sbjct: 123 ARRGSQRTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGE 182

Query: 182 KFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL 241
           KFEKPKTK+F+AA++RWG+DPKEK++FLM +V +NV  SSRNIGTL++LTPRTLNLFDIL
Sbjct: 183 KFEKPKTKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDIL 242

Query: 242 DSDKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEE 276
           ++DK+VLTPAAV++LN RYG++     +DDE+E EEE
Sbjct: 243 NADKLVLTPAAVEFLNARYGVDAVEEEDDDEDETEEE 279

BLAST of MC02g0731 vs. TAIR 10
Match: AT1G07320.3 (ribosomal protein L4 )

HSP 1 Score: 367.1 bits (941), Expect = 1.3e-101
Identity = 191/275 (69.45%), Postives = 233/275 (84.73%), Query Frame = 0

Query: 2   ATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAGE 61
           +++T P+SLSFF+SS+FLSS++Q  K +           +KPLS+SSQLATLP+LSF GE
Sbjct: 3   SSATAPNSLSFFSSSLFLSSSHQIPKTYISVSKLGSGRVSKPLSVSSQLATLPILSFEGE 62

Query: 62  KVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTGK 121
           KVGETYLDLK+AP +TARAVVHRAI+TD  NKRRGTASTLTR EV GGG KPY QKKTG 
Sbjct: 63  KVGETYLDLKAAPEDTARAVVHRAIVTDLNNKRRGTASTLTRGEVRGGGIKPYSQKKTGH 122

Query: 122 ARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAV---NTIVVEDFGD 181
           ARRGS RTPLRPGGGVVFGP+P+DWSIKINRKEK+LAISTA++SAA      IVVE+FG+
Sbjct: 123 ARRGSQRTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGE 182

Query: 182 KFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL 241
           KFEKPKTK+F+AA++RWG+DPKEK++FLM +V +NV  SSRNIGTL++LTPRTLNLFDIL
Sbjct: 183 KFEKPKTKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDIL 242

Query: 242 DSDKMVLTPAAVDYLNERYGINYEGGIEDDEEEEE 274
           ++DK+VLTPAAV++LN RYG++     +DDE+E E
Sbjct: 243 NADKLVLTPAAVEFLNARYGVDAVEEEDDDEDETE 277

BLAST of MC02g0731 vs. TAIR 10
Match: AT1G07320.4 (ribosomal protein L4 )

HSP 1 Score: 367.1 bits (941), Expect = 1.3e-101
Identity = 191/275 (69.45%), Postives = 233/275 (84.73%), Query Frame = 0

Query: 2   ATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAGE 61
           +++T P+SLSFF+SS+FLSS++Q  K +           +KPLS+SSQLATLP+LSF GE
Sbjct: 3   SSATAPNSLSFFSSSLFLSSSHQIPKTYISVSKLGSGRVSKPLSVSSQLATLPILSFEGE 62

Query: 62  KVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTGK 121
           KVGETYLDLK+AP +TARAVVHRAI+TD  NKRRGTASTLTR EV GGG KPY QKKTG 
Sbjct: 63  KVGETYLDLKAAPEDTARAVVHRAIVTDLNNKRRGTASTLTRGEVRGGGIKPYSQKKTGH 122

Query: 122 ARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAV---NTIVVEDFGD 181
           ARRGS RTPLRPGGGVVFGP+P+DWSIKINRKEK+LAISTA++SAA      IVVE+FG+
Sbjct: 123 ARRGSQRTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGE 182

Query: 182 KFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL 241
           KFEKPKTK+F+AA++RWG+DPKEK++FLM +V +NV  SSRNIGTL++LTPRTLNLFDIL
Sbjct: 183 KFEKPKTKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDIL 242

Query: 242 DSDKMVLTPAAVDYLNERYGINYEGGIEDDEEEEE 274
           ++DK+VLTPAAV++LN RYG++     +DDE+E E
Sbjct: 243 NADKLVLTPAAVEFLNARYGVDAVEEEDDDEDETE 277

BLAST of MC02g0731 vs. TAIR 10
Match: AT1G07320.1 (ribosomal protein L4 )

HSP 1 Score: 365.5 bits (937), Expect = 3.7e-101
Identity = 191/279 (68.46%), Postives = 235/279 (84.23%), Query Frame = 0

Query: 2   ATSTTPSSLSFFTSSVFLSSANQNSKLFCHWKPNSLKPQTKPLSISSQLATLPVLSFAGE 61
           +++T P+SLSFF+SS+FLSS++Q  K +           +KPLS+SSQLATLP+LSF GE
Sbjct: 3   SSATAPNSLSFFSSSLFLSSSHQIPKTYISVSKLGSGRVSKPLSVSSQLATLPILSFEGE 62

Query: 62  KVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKKPYQQKKTGK 121
           KVGETYLDLK+AP +TARAVVHRAI+TD  NKRRGTASTLTR EV GGG KPY QKKTG 
Sbjct: 63  KVGETYLDLKAAPEDTARAVVHRAIVTDLNNKRRGTASTLTRGEVRGGGIKPYSQKKTGH 122

Query: 122 ARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAV---NTIVVEDFGD 181
           ARRGS RTPLRPGGGVVFGP+P+DWSIKINRKEK+LAISTA++SAA      IVVE+FG+
Sbjct: 123 ARRGSQRTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGE 182

Query: 182 KFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDIL 241
           KFEKPKTK+F+AA++RWG+DPKEK++FLM +V +NV  SSRNIGTL++LTPRTLNLFDIL
Sbjct: 183 KFEKPKTKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDIL 242

Query: 242 DSDKMVLTPAAVDYLNERYGINYEGGIEDDEEEEEEEQQ 278
           ++DK+VLTPAAV++LN RYG++     +DDE+E E  ++
Sbjct: 243 NADKLVLTPAAVEFLNARYGVDAVEEEDDDEDETEGSEE 281

BLAST of MC02g0731 vs. TAIR 10
Match: AT2G20060.1 (Ribosomal protein L4/L1 family )

HSP 1 Score: 92.8 bits (229), Expect = 4.6e-19
Identity = 61/206 (29.61%), Postives = 112/206 (54.37%), Query Frame = 0

Query: 53  LPVLSFAGEKVGETYLDLKSAPPETARAVVHRAIITDQQNKRRGTASTLTRAEVSGGGKK 112
           +PV +F  E+ G   L          + ++H  +      +++GT ST T +EVSG G+K
Sbjct: 91  IPVTNFQNEEKGFMVLAGDVFDVPIRKDIIHNVVRWQLAKRQQGTHSTKTLSEVSGTGRK 150

Query: 113 PYQQKKTGKARRGSMRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAV--NT 172
           P+ QK TG+AR G++R P   GG V+ GPKPR  +IK+N++ +RL +  A+ + A     
Sbjct: 151 PWNQKGTGRARHGTLRGPQFRGGCVMHGPKPRSHAIKMNKQVRRLGLKIALTARAAEGKL 210

Query: 173 IVVEDFGDKFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPR 232
           +V +D G      KTK  +    +   + K+  +     + + ++L+++N+  + ++   
Sbjct: 211 LVFDDLG--LPTHKTKNIVNYYNQME-NTKKVLVVEGGPIDEKLKLATQNLHYVNIIPSI 270

Query: 233 TLNLFDILDSDKMVLTPAAVDYLNER 257
            LN++ IL  D ++++  AV+ + ER
Sbjct: 271 GLNVYSILLHDTLLMSRDAVNKIVER 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O500615.2e-10068.4650S ribosomal protein L4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL4 ... [more]
O803611.3e-9569.4950S ribosomal protein L4, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL4 PE=... [more]
O499372.0e-9163.5750S ribosomal protein L4, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL4 PE=... [more]
Q8DMN01.3e-5553.4350S ribosomal protein L4 OS=Thermosynechococcus elongatus (strain BP-1) OX=19722... [more]
B0JI023.9e-5551.4750S ribosomal protein L4 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 G... [more]
Match NameE-valueIdentityDescription
XP_022148353.11.00e-189100.0050S ribosomal protein L4, chloroplastic [Momordica charantia][more]
XP_023532270.14.18e-17994.6050S ribosomal protein L4, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022948154.12.42e-17894.2450S ribosomal protein L4, chloroplastic-like isoform X1 [Cucurbita moschata] >KA... [more]
XP_022948155.12.42e-17894.2450S ribosomal protein L4, chloroplastic-like isoform X2 [Cucurbita moschata][more]
XP_023007555.11.91e-17794.8950S ribosomal protein L4, chloroplastic-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1D3V74.86e-190100.0050S ribosomal protein L4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1G8Y31.17e-17894.2450S ribosomal protein L4, chloroplastic-like isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1G9171.17e-17894.2450S ribosomal protein L4, chloroplastic-like isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1KZ169.27e-17894.8950S ribosomal protein L4, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3... [more]
A0A6J1L3A59.27e-17894.8950S ribosomal protein L4, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3... [more]
Match NameE-valueIdentityDescription
AT1G07320.22.6e-10269.68ribosomal protein L4 [more]
AT1G07320.31.3e-10169.45ribosomal protein L4 [more]
AT1G07320.41.3e-10169.45ribosomal protein L4 [more]
AT1G07320.13.7e-10168.46ribosomal protein L4 [more]
AT2G20060.14.6e-1929.61Ribosomal protein L4/L1 family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR01300550S ribosomal protein uL4TIGRFAMTIGR03953TIGR03953coord: 76..254
e-value: 1.3E-57
score: 192.2
IPR01300550S ribosomal protein uL4PANTHERPTHR1074650S RIBOSOMAL PROTEIN L4coord: 2..273
IPR01300550S ribosomal protein uL4HAMAPMF_01328_BRibosomal_L4_Bcoord: 58..258
score: 29.44725
IPR002136Ribosomal protein L4/L1ePFAMPF00573Ribosomal_L4coord: 77..256
e-value: 2.5E-53
score: 180.6
IPR023574Ribosomal protein L4 domain superfamilyGENE3D3.40.1370.10coord: 36..263
e-value: 2.6E-60
score: 205.4
IPR023574Ribosomal protein L4 domain superfamilySUPERFAMILY52166Ribosomal protein L4coord: 52..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0731.1MC02g0731.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005840 ribosome
molecular_function GO:0003735 structural constituent of ribosome