MC02g0659 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0659
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAcid phosphatase/vanadium-dependent haloperoxidase-related protein
LocationMC02: 5324024 .. 5328200 (+)
RNA-Seq ExpressionMC02g0659
SyntenyMC02g0659
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAAGGTGTTCCCTTGTTCACGACGGACAAACACGACCCACTTGGACTCCAAAAGAAAACAGACTAATAACGGCCGCTGCCCTGAGAAGTTAAATCGAAAACTCATTGGTTTGGATATATTCCTCAGGCTTCTTGCCGCCATTGCAGACGGAAGAGGAGAATGTCACTGCCATGGTGGAGCTCCGCCACCGCCGTTCCTTTTCCGTCTCAGCCGCGGCTAATTACTCATCATCGCCACTACTCTCACATTCATCTCAGAAAATTATGCCCTTCTCCTTCCAATTTCACCGCTCCCAGAGCTGTATTGCTTGACGAAATCGTTCAGCTCAGCCATAACAAGGTAATTCCTTCCCCTTAATCTCACTCAGTACCGATATGGCCTCTCTCTCTTGTTTTCAGCGGAGGAATCTTGCCGTAAATTTTGCTCAATTTGTTTTCAAGGTTTTAGTTGCCGCCGGTGTGTCTGCTGCGATCGGGCAACTGGCGAAGCCTTTCGCTTCTGTTATTATCTATGGCAGAGATTTCAACTTCAGAACCGCCTTCGAGGCTGGAGGCTTCCCTTCTACCCATTCCTCTGTTAGTAGACCCATTTGTTCTGTAATTTTCAATTCTTTTGTTAATGTAATCACCATCATCACTGCTGTTTTCGTGAAAATGTTTCAGGCGGTGGTCGCTGCTGCGACAATCCTCGGCGCTGAGAGGTAATCAGCTACTTATTATGTGCTTTTTTTGTCGTCTGTGTTTCCGTTTTAAGAAATCATGTACCTGTGTTTTAGAATTACTTGTCATAACATTACTTAAGAATATAAACGCAGGGGTCTTGCAGATTCTATTTTTGGCCTAACTGTAGTTTACGCGTCCCTTATTATGTACGATGCTCAGGCAAGTATGAAGGGTCTTCTCCTTCTTTTTCTTTTTTATTTTTTGTTTGGCCCTCGTTATACTTGATTATAATCGTCTTCCCTTTTCTTTTCCTGGCATCTTTCTGCTGATTAATTGTGATTTTTTCCCTATTTTCTTCTGCTAAATATTCTGATGCGAACTTCTATTAACGAGGAACTTCGATGGAAAGAGAAAATGATCTCATAATTTATCATCTGGGCACCGGTATTATTGAGTGAAGCAATACTCTTGAACAAGTGTGAGTAATCAAATGAAGTAAAGCATTAAGGCCCTATATTAAAATTATCATCAGAAAATCCAACTGTGACACTGTATAATAGGCTAAACTTTTTTTAATATCTTTTTGAGCAAGAGTTAGCATAGTAGCAATAGTAGCAGTAGCAGCAGCAGTGGCAATAGTAGCATATCAGTAGTAAGTAGTGTAGCAGCAGCAGCAGTACTACTGTTCGTTTCGTTTGGCTTCTGCTCCTATTAGTATGTTCTCTTGAAAGATGCCAGTTTAGACCATTTATGGTGGCTCTTCTACCTTCATTTGTAGTAAATCATTTTTCATCTTTGATATCTGTTAGGTGAGTCGGTGCAGTAGCTGAGTGTAAGTCGTTTAACAGTTTGCAAAATGAAGAGGCAAAACTGGCTCCATGCCTTCTTTCTAGGATACATGGTTGGTGAGATATAGGTGTGTTCTTGAGGACTAAGGCTCTAATGTCTTAAGAACTAGGGAGAATTCACTTTATTATCATGTCACAGGTTTACTCCTTTATATGGATTGGTATCCATTGTGAAAATGTTCAGAAACATTCCTCGTGACTGATGGATAGTAAACGAAGAGAACATGCTTGTAATTCTTCGGTGGCCTTTCTGAGCTAGGTGTATTTTAATTGTACAGGTTCCTTCTTTTTGGATTCCTTGTCAACTCATCTTTTTAATTTTGCCTCCTTTATGAAACTATTTTTTAAAATAAAATAAAAATCTGGGTATTATAATAGTAATATGTTTGGAGAAGTTATAGTCATTATTTACCAGCCCAATTGGCATAGAAAAACTACCGTTTTACTAGTTGCATCCGGAATTCATCATAGAAACTTTTTTTTTTTAAATCTTACATGCTCATAGTGTGCCAAAGTTTCTTCTTTCCTGTTGGTTTATTTTAGTCTTCTTCATTTCTATGTTTCATGTTTTGATCTTACAGTCATGTTGTTTAGGATATCATGCTTAATTGTTGCTCTGACCTTGTAGAAGTTGAACAAATAATGTTGAGCTAGATATTAGGGGTCTGCGTCGTTTGGATATTATTAGATTATCTGTTATCCGAAAGTGTTATAGCTTGCCATGCAACATAGTAGGCATAGGAGGCAAAACTGGACTATTGAATTTAGGCAGATAAATGCTCTTATGTTAACTGATATATAATATAATATAAGTGAATATAGAAATACTCGCTTAGATCTTGGAACTAGTTTATCTGCTGGCCCCATTTCAAGCATATTTGGATCTAAAATACTAACTATTACGATATATTTTACATCCCTTGGCTTCTGCTGAGATCACTTACACTCAGCAAGATTCTTTTCTGTAGTATCTAAGCATAGGAAGTTACACTCCCAAAGGAAAATAATTTATGGAAATGATAAGTATGTTCATATCTGTATGTATGTGCATATGTGCACGTATATGTGTATGCATGTATACAGTGTGTAATAGTTAGCAACTTTAGAGAGTTTAGACCCCTACTGCAGTTTATTAGATTATGTTCTAAAGTTGGACTTAAACAGGTTCTCCCCTCCACCAGCCAATCTACCTTTGTTGGCTGTAGGCAGATCGTAGACCTCATCTTAATTGAAAAAATGTGGTGGATGATTCCAAAAGAAGAGACAAAAAGGGGATGGTTTTTAATTGCATCTTCAGAATACTTGTGATAAAGTTGACTGGTTTTCCCTTGATTTCCATCCTTCAAGTAGAAGGCTTTAGGGAAAGGTGGAGGAAGTTGATATTGGCTATAATCTCGAGCACTAACTTCTCGATTATATTGTAAATCAAACCTAGATCACTAGATAGTTTCATGCTTCTAGAGATCTTTGGGAAGGAGATCCCTTATTCTCTTTTCTTTTAGTATTTGCAGCAGATTATCTTAGTCAGTTACCTTATAGAGGTTTGGTGGTTCGTGTGGTGGAATCCATCAGGTTTGCATAATTTGGGCAACGATAGGTTTACGAATCGATTGGTGGAGATATTATGTCATTATGACAATCATTTTTGACGTATCTTCGTTCTGACCAGCTTGGGAGTAGGTTGTACTGTCCGGTACGGACTTTATGCCAGGCTTAGGCTATCCACCTCCCCCAAGGGATGAGCTCCCACTACATCGGTCGTTCATACTTGGCTAATGATATCTAGTATTTTATTTGTACAATCACTCAAAATTGAAAAATATACTTAATATCGTTAGACTTTTCGAGAAGGCCTCGGGGACTACTTGTATCTAGGAGAAGTCAAAATTGTGGTGGATTGGTTTGTTTGTGGTGCGGGAAGCTGGCCTATGATCTTGGGCCTCCTCTTCAGGGAGATCAGAAGAGTTTTTAGTGAACTTCCCATTTTTTGAGTTAAAAAACAAGCTGGGGTAGGAATTCGAACCTCCAACCTTTTGGTCGAGAATACATGTCTTTAAGCAATTGATACTTAGGTTAGCTAACAAACTCCACCTTTTTCCCGAGGAAGATCCACTCGTTTGCAGTTCTAGCCTCCCTATATACATTCTTTCCATTTTTAGAATCTCCTGCAGTATAGTATTAGGAACAGAATCGAAAAACGCTCAGAAATTTCTCATGGGAGGGCCCTGCTTTTTTTTGGTATCAGTAAGAGTAACCAATAACAAAGTTTCTCATTTTGCAGGGAGTGAGGAGGGAAGTAGGAAATCATGCTAAAGCCATCAACAAGCTGTTGCAGACAAAGACACAAGTGACCTCGTCATTTCCCTGTAAAGATCAAGATCATCGAGCCGATTCCCTGCTCGAAAATAGAACATCACCACTCAATAGAAACCTTGAGAGCAGCAGTCCCATATTATCCGAGGAAGGAACCAAGACCTTAAAAGTTCCGAGTCCAGTAAAGGAGGATATTGCATCCTCAAGCTTGGCAGCAGATATGGAAGAAGGGTCAAGAACAAAGGCTAATCTTTGGAAGCCATTTAAGGAATCAATTGGCCACACTGAGGTTGAAGTTGCAGCTGGTGCTTTGTTGGGTTTCACGGTTAGCCTGATAACCAATACATTATTG

mRNA sequence

CAAAAGGTGTTCCCTTGTTCACGACGGACAAACACGACCCACTTGGACTCCAAAAGAAAACAGACTAATAACGGCCGCTGCCCTGAGAAGTTAAATCGAAAACTCATTGGTTTGGATATATTCCTCAGGCTTCTTGCCGCCATTGCAGACGGAAGAGGAGAATGTCACTGCCATGGTGGAGCTCCGCCACCGCCGTTCCTTTTCCGTCTCAGCCGCGGCTAATTACTCATCATCGCCACTACTCTCACATTCATCTCAGAAAATTATGCCCTTCTCCTTCCAATTTCACCGCTCCCAGAGCTGTATTGCTTGACGAAATCGTTCAGCTCAGCCATAACAAGGTTTTAGTTGCCGCCGGTGTGTCTGCTGCGATCGGGCAACTGGCGAAGCCTTTCGCTTCTGTTATTATCTATGGCAGAGATTTCAACTTCAGAACCGCCTTCGAGGCTGGAGGCTTCCCTTCTACCCATTCCTCTGCGGTGGTCGCTGCTGCGACAATCCTCGGCGCTGAGAGGGGTCTTGCAGATTCTATTTTTGGCCTAACTGTAGTTTACGCGTCCCTTATTATGTACGATGCTCAGGCAAGTATGAAGGGTCTTCTCCTTCTTTTTCTTTTTTATTTTTTGTTTGGCCCTCTGAGGAGGGAAGTAGGAAATCATGCTAAAGCCATCAACAAGCTGTTGCAGACAAAGACACAAGTGACCTCGTCATTTCCCTGTAAAGATCAAGATCATCGAGCCGATTCCCTGCTCGAAAATAGAACATCACCACTCAATAGAAACCTTGAGAGCAGCAGTCCCATATTATCCGAGGAAGGAACCAAGACCTTAAAAGTTCCGAGTCCAGTAAAGGAGGATATTGCATCCTCAAGCTTGGCAGCAGATATGGAAGAAGGGTCAAGAACAAAGGCTAATCTTTGGAAGCCATTTAAGGAATCAATTGGCCACACTGAGGTTGAAGTTGCAGCTGGTGCTTTGTTGGGTTTCACGGTTAGCCTGATAACCAATACATTATTG

Coding sequence (CDS)

ATGTCACTGCCATGGTGGAGCTCCGCCACCGCCGTTCCTTTTCCGTCTCAGCCGCGGCTAATTACTCATCATCGCCACTACTCTCACATTCATCTCAGAAAATTATGCCCTTCTCCTTCCAATTTCACCGCTCCCAGAGCTGTATTGCTTGACGAAATCGTTCAGCTCAGCCATAACAAGGTTTTAGTTGCCGCCGGTGTGTCTGCTGCGATCGGGCAACTGGCGAAGCCTTTCGCTTCTGTTATTATCTATGGCAGAGATTTCAACTTCAGAACCGCCTTCGAGGCTGGAGGCTTCCCTTCTACCCATTCCTCTGCGGTGGTCGCTGCTGCGACAATCCTCGGCGCTGAGAGGGGTCTTGCAGATTCTATTTTTGGCCTAACTGTAGTTTACGCGTCCCTTATTATGTACGATGCTCAGGCAAGTATGAAGGGTCTTCTCCTTCTTTTTCTTTTTTATTTTTTGTTTGGCCCTCTGAGGAGGGAAGTAGGAAATCATGCTAAAGCCATCAACAAGCTGTTGCAGACAAAGACACAAGTGACCTCGTCATTTCCCTGTAAAGATCAAGATCATCGAGCCGATTCCCTGCTCGAAAATAGAACATCACCACTCAATAGAAACCTTGAGAGCAGCAGTCCCATATTATCCGAGGAAGGAACCAAGACCTTAAAAGTTCCGAGTCCAGTAAAGGAGGATATTGCATCCTCAAGCTTGGCAGCAGATATGGAAGAAGGGTCAAGAACAAAGGCTAATCTTTGGAAGCCATTTAAGGAATCAATTGGCCACACTGAGGTTGAAGTTGCAGCTGGTGCTTTGTTGGGTTTCACGGTTAGCCTGATAACCAATACATTATTG

Protein sequence

MSLPWWSSATAVPFPSQPRLITHHRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHNKVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERGLADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQVTSSFPCKDQDHRADSLLENRTSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSLAADMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL
Homology
BLAST of MC02g0659 vs. ExPASy Swiss-Prot
Match: O32107 (Uncharacterized membrane protein YuiD OS=Bacillus subtilis (strain 168) OX=224308 GN=yuiD PE=4 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 6.5e-05
Identity = 33/103 (32.04%), Postives = 48/103 (46.60%), Query Frame = 0

Query: 78  FASVI------IYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERGLADSIFGLTVVY 137
           FA VI      I  R  ++      GG PS+HS+AV A +T +  E GL  S+F ++ ++
Sbjct: 18  FAQVIKVPIQFIVSRKLDWSLVTSTGGMPSSHSAAVTALSTGVALEHGLDSSLFAVSAIF 77

Query: 138 ASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLL 175
           A + M+DA                   +RR  G  A  INKL+
Sbjct: 78  AVITMFDATG-----------------VRRHAGEQATVINKLV 103

BLAST of MC02g0659 vs. NCBI nr
Match: XP_022149578.1 (uncharacterized protein LOC111017975 [Momordica charantia])

HSP 1 Score: 503 bits (1295), Expect = 9.37e-179
Identity = 265/285 (92.98%), Postives = 266/285 (93.33%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITHHRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHNK 60
           MSLPWWSSATAVPFPSQPRLITHHRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHNK
Sbjct: 1   MSLPWWSSATAVPFPSQPRLITHHRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHNK 60

Query: 61  VLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERGL 120
           VLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERGL
Sbjct: 61  VLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERGL 120

Query: 121 ADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQV 180
           ADSIFGLTVVYASLIMYDAQ                  +RREVGNHAKAINKLLQTKTQV
Sbjct: 121 ADSIFGLTVVYASLIMYDAQG-----------------VRREVGNHAKAINKLLQTKTQV 180

Query: 181 TSSFPCKDQDHRADSLLENRTSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSLAA 240
           TSSFPCKDQDHRAD LLENRTSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSLAA
Sbjct: 181 TSSFPCKDQDHRADXLLENRTSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSLAA 240

Query: 241 DMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           DMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL
Sbjct: 241 DMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 268

BLAST of MC02g0659 vs. NCBI nr
Match: XP_038901649.1 (uncharacterized protein LOC120088431 [Benincasa hispida])

HSP 1 Score: 395 bits (1014), Expect = 6.76e-136
Identity = 217/289 (75.09%), Postives = 236/289 (81.66%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITH---HRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLS 60
           MSL W SSATAVPFPSQ RLITH   H+HY HI LRKL PSPS FTAPRA+LLDEIVQL+
Sbjct: 1   MSLSWCSSATAVPFPSQTRLITHPSHHQHYFHIRLRKLSPSPSIFTAPRAILLDEIVQLT 60

Query: 61  HNKVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAE 120
           HNKVLV+AGVSAAIGQLAKPF SV+ YGR+FN RTAFEAGGFPSTHSSAVVAAATILGAE
Sbjct: 61  HNKVLVSAGVSAAIGQLAKPFTSVVFYGREFNIRTAFEAGGFPSTHSSAVVAAATILGAE 120

Query: 121 RGLADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTK 180
           RGLADSIFG+TVVYASLIMYDAQ                  +RREVG HAKAINKLLQT+
Sbjct: 121 RGLADSIFGITVVYASLIMYDAQG-----------------VRREVGKHAKAINKLLQTE 180

Query: 181 TQVTSSFPCKDQDHRADSLLENR-TSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASS 240
           T V SSFP K+QD R DS LE R +S LNRNLE  SPILSEE TK L  PSPVK+D+ASS
Sbjct: 181 TPVNSSFPYKEQDLRVDSQLEKRVSSSLNRNLEIGSPILSEESTKALMAPSPVKQDVASS 240

Query: 241 SLAADMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
            +A D+EE SR +A+ WKPFKESIGHTE+EVAAGALLGFTVSLIT +LL
Sbjct: 241 GIANDLEEASRMEASRWKPFKESIGHTEIEVAAGALLGFTVSLITISLL 272

BLAST of MC02g0659 vs. NCBI nr
Match: KAA0033550.1 (Acid phosphatase/vanadium-dependent haloperoxidase-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 385 bits (988), Expect = 7.82e-132
Identity = 214/288 (74.31%), Postives = 235/288 (81.60%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITHHRHYSH-IHLRKLCPSPSNFTAPRAVLLDEIVQLSHN 60
           MSLPWW SATAVPFPSQ +LI+H  H+ H I LRKL  SPS  TAPRAVLLDEIVQL+HN
Sbjct: 1   MSLPWWISATAVPFPSQTQLISHPCHHQHHIRLRKLSSSPSTSTAPRAVLLDEIVQLTHN 60

Query: 61  KVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERG 120
           KVL+AAGVSAAIGQLAKPF SV+ YGR+FN RTAFEAGGFPSTHSSAVVAAATILGAERG
Sbjct: 61  KVLIAAGVSAAIGQLAKPFTSVVFYGREFNIRTAFEAGGFPSTHSSAVVAAATILGAERG 120

Query: 121 LADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQ 180
           LADSIFG+TVVYASLIMYDAQAS K L L          +RREVG HAKA+NKL QT+  
Sbjct: 121 LADSIFGITVVYASLIMYDAQASTKALPL---------GVRREVGKHAKALNKLSQTERP 180

Query: 181 VTSSFPCKDQDHRADSLLENRTS-PLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSL 240
           + SS P KDQD R DSL E R S  LNRNLE  +PILSEE TK L VPSPVK+D+ SSS+
Sbjct: 181 MNSSSPYKDQDLRVDSLFEKRISLSLNRNLEIGNPILSEESTKALTVPSPVKQDVTSSSV 240

Query: 241 AADMEEGSRTKANL-WKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           A D+EEGS  +A+  WKPFKESIGHTE+EVAAGALLGFTVSLITN+ L
Sbjct: 241 ANDLEEGSIKEASSSWKPFKESIGHTEIEVAAGALLGFTVSLITNSFL 279

BLAST of MC02g0659 vs. NCBI nr
Match: KAE8646867.1 (hypothetical protein Csa_021036 [Cucumis sativus])

HSP 1 Score: 383 bits (983), Expect = 3.40e-131
Identity = 210/288 (72.92%), Postives = 236/288 (81.94%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITH-HRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHN 60
           MSLPWW S TAVPFPSQ +LI+H ++H+SHI LRKL  SPS  TAPRAVLLDEIVQL+HN
Sbjct: 1   MSLPWWISPTAVPFPSQTQLISHPYQHHSHIRLRKLSSSPSTSTAPRAVLLDEIVQLTHN 60

Query: 61  KVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERG 120
           KVLVAAGVSAAIGQLAKPF SV+ YGR+FN RTAFEAGGFPSTHSSAVVAAATILGAERG
Sbjct: 61  KVLVAAGVSAAIGQLAKPFTSVVFYGREFNIRTAFEAGGFPSTHSSAVVAAATILGAERG 120

Query: 121 LADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQ 180
           LADSIFG+TVVYASLIMYDAQA                 +RREVG H+KA+NKL QT+  
Sbjct: 121 LADSIFGITVVYASLIMYDAQA-----------------IRREVGKHSKALNKLSQTERP 180

Query: 181 VTSSFPCKDQDHRADSLLENR-TSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSL 240
           + SSFP KD+D R DS LE R +S LNRNLE  SP+LSEE TK L VPSPVK+D+ +SS+
Sbjct: 181 MNSSFPYKDEDLRVDSQLEKRISSSLNRNLEIGSPMLSEESTKALTVPSPVKQDVTTSSV 240

Query: 241 AADMEEGSRTKANL-WKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           A D+E GSR +A+  WKPFKESIGHTE+EVAAGALLGFTVSLITN+LL
Sbjct: 241 ANDLEGGSRMEASSSWKPFKESIGHTEIEVAAGALLGFTVSLITNSLL 271

BLAST of MC02g0659 vs. NCBI nr
Match: XP_004139920.1 (uncharacterized protein LOC101219161 [Cucumis sativus])

HSP 1 Score: 381 bits (978), Expect = 1.96e-130
Identity = 209/288 (72.57%), Postives = 235/288 (81.60%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITH-HRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHN 60
           MSLPWW S TAVPFPSQ +LI+H ++H+SHI LRKL  SPS  TAPRAVLLDEIVQL+HN
Sbjct: 1   MSLPWWISPTAVPFPSQTQLISHPYQHHSHIRLRKLSSSPSTSTAPRAVLLDEIVQLTHN 60

Query: 61  KVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERG 120
           KVLVAAGVSAAIGQLAKPF SV+ YGR+FN RTAFEAGGFPSTHSSAVVAAATILGAERG
Sbjct: 61  KVLVAAGVSAAIGQLAKPFTSVVFYGREFNIRTAFEAGGFPSTHSSAVVAAATILGAERG 120

Query: 121 LADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQ 180
           LADSIFG+TVVYASLIMYDAQ                  +RREVG H+KA+NKL QT+  
Sbjct: 121 LADSIFGITVVYASLIMYDAQG-----------------VRREVGKHSKALNKLSQTERP 180

Query: 181 VTSSFPCKDQDHRADSLLENR-TSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSL 240
           + SSFP KD+D R DS LE R +S LNRNLE  SP+LSEE TK L VPSPVK+D+ +SS+
Sbjct: 181 MNSSFPYKDEDLRVDSQLEKRISSSLNRNLEIGSPMLSEESTKALTVPSPVKQDVTTSSV 240

Query: 241 AADMEEGSRTKANL-WKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           A D+E GSR +A+  WKPFKESIGHTE+EVAAGALLGFTVSLITN+LL
Sbjct: 241 ANDLEGGSRMEASSSWKPFKESIGHTEIEVAAGALLGFTVSLITNSLL 271

BLAST of MC02g0659 vs. ExPASy TrEMBL
Match: A0A6J1D762 (uncharacterized protein LOC111017975 OS=Momordica charantia OX=3673 GN=LOC111017975 PE=4 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 4.54e-179
Identity = 265/285 (92.98%), Postives = 266/285 (93.33%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITHHRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHNK 60
           MSLPWWSSATAVPFPSQPRLITHHRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHNK
Sbjct: 1   MSLPWWSSATAVPFPSQPRLITHHRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHNK 60

Query: 61  VLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERGL 120
           VLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERGL
Sbjct: 61  VLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERGL 120

Query: 121 ADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQV 180
           ADSIFGLTVVYASLIMYDAQ                  +RREVGNHAKAINKLLQTKTQV
Sbjct: 121 ADSIFGLTVVYASLIMYDAQG-----------------VRREVGNHAKAINKLLQTKTQV 180

Query: 181 TSSFPCKDQDHRADSLLENRTSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSLAA 240
           TSSFPCKDQDHRAD LLENRTSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSLAA
Sbjct: 181 TSSFPCKDQDHRADXLLENRTSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSLAA 240

Query: 241 DMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           DMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL
Sbjct: 241 DMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 268

BLAST of MC02g0659 vs. ExPASy TrEMBL
Match: A0A5A7SRL4 (Acid phosphatase/vanadium-dependent haloperoxidase-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold261G001010 PE=4 SV=1)

HSP 1 Score: 385 bits (988), Expect = 3.79e-132
Identity = 214/288 (74.31%), Postives = 235/288 (81.60%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITHHRHYSH-IHLRKLCPSPSNFTAPRAVLLDEIVQLSHN 60
           MSLPWW SATAVPFPSQ +LI+H  H+ H I LRKL  SPS  TAPRAVLLDEIVQL+HN
Sbjct: 1   MSLPWWISATAVPFPSQTQLISHPCHHQHHIRLRKLSSSPSTSTAPRAVLLDEIVQLTHN 60

Query: 61  KVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERG 120
           KVL+AAGVSAAIGQLAKPF SV+ YGR+FN RTAFEAGGFPSTHSSAVVAAATILGAERG
Sbjct: 61  KVLIAAGVSAAIGQLAKPFTSVVFYGREFNIRTAFEAGGFPSTHSSAVVAAATILGAERG 120

Query: 121 LADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQ 180
           LADSIFG+TVVYASLIMYDAQAS K L L          +RREVG HAKA+NKL QT+  
Sbjct: 121 LADSIFGITVVYASLIMYDAQASTKALPL---------GVRREVGKHAKALNKLSQTERP 180

Query: 181 VTSSFPCKDQDHRADSLLENRTS-PLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSL 240
           + SS P KDQD R DSL E R S  LNRNLE  +PILSEE TK L VPSPVK+D+ SSS+
Sbjct: 181 MNSSSPYKDQDLRVDSLFEKRISLSLNRNLEIGNPILSEESTKALTVPSPVKQDVTSSSV 240

Query: 241 AADMEEGSRTKANL-WKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           A D+EEGS  +A+  WKPFKESIGHTE+EVAAGALLGFTVSLITN+ L
Sbjct: 241 ANDLEEGSIKEASSSWKPFKESIGHTEIEVAAGALLGFTVSLITNSFL 279

BLAST of MC02g0659 vs. ExPASy TrEMBL
Match: A0A0A0KB68 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G134360 PE=4 SV=1)

HSP 1 Score: 381 bits (978), Expect = 9.48e-131
Identity = 209/288 (72.57%), Postives = 235/288 (81.60%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITH-HRHYSHIHLRKLCPSPSNFTAPRAVLLDEIVQLSHN 60
           MSLPWW S TAVPFPSQ +LI+H ++H+SHI LRKL  SPS  TAPRAVLLDEIVQL+HN
Sbjct: 1   MSLPWWISPTAVPFPSQTQLISHPYQHHSHIRLRKLSSSPSTSTAPRAVLLDEIVQLTHN 60

Query: 61  KVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERG 120
           KVLVAAGVSAAIGQLAKPF SV+ YGR+FN RTAFEAGGFPSTHSSAVVAAATILGAERG
Sbjct: 61  KVLVAAGVSAAIGQLAKPFTSVVFYGREFNIRTAFEAGGFPSTHSSAVVAAATILGAERG 120

Query: 121 LADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQ 180
           LADSIFG+TVVYASLIMYDAQ                  +RREVG H+KA+NKL QT+  
Sbjct: 121 LADSIFGITVVYASLIMYDAQG-----------------VRREVGKHSKALNKLSQTERP 180

Query: 181 VTSSFPCKDQDHRADSLLENR-TSPLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSL 240
           + SSFP KD+D R DS LE R +S LNRNLE  SP+LSEE TK L VPSPVK+D+ +SS+
Sbjct: 181 MNSSFPYKDEDLRVDSQLEKRISSSLNRNLEIGSPMLSEESTKALTVPSPVKQDVTTSSV 240

Query: 241 AADMEEGSRTKANL-WKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           A D+E GSR +A+  WKPFKESIGHTE+EVAAGALLGFTVSLITN+LL
Sbjct: 241 ANDLEGGSRMEASSSWKPFKESIGHTEIEVAAGALLGFTVSLITNSLL 271

BLAST of MC02g0659 vs. ExPASy TrEMBL
Match: A0A1S3C2U0 (uncharacterized protein LOC103496264 OS=Cucumis melo OX=3656 GN=LOC103496264 PE=4 SV=1)

HSP 1 Score: 376 bits (966), Expect = 6.34e-129
Identity = 209/288 (72.57%), Postives = 230/288 (79.86%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITHHRHYSH-IHLRKLCPSPSNFTAPRAVLLDEIVQLSHN 60
           MSLPWW SATAVPFPSQ +LI+H  H+ H I LRKL  SPS  TAPRAVLLDEIVQL+HN
Sbjct: 1   MSLPWWISATAVPFPSQTQLISHPCHHQHHIRLRKLSSSPSTSTAPRAVLLDEIVQLTHN 60

Query: 61  KVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAERG 120
           KVL+AAGVSAAIGQLAKPF SV+ YGR+FN RTAFEAGGFPSTHSSAVVAAATILGAERG
Sbjct: 61  KVLIAAGVSAAIGQLAKPFTSVVFYGREFNIRTAFEAGGFPSTHSSAVVAAATILGAERG 120

Query: 121 LADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTKTQ 180
           LADSIFG+TVVYASLIMYDAQ                  +RREVG HAKA+NKL QT+  
Sbjct: 121 LADSIFGITVVYASLIMYDAQG-----------------VRREVGKHAKALNKLSQTERP 180

Query: 181 VTSSFPCKDQDHRADSLLENRTS-PLNRNLESSSPILSEEGTKTLKVPSPVKEDIASSSL 240
           + SS P KDQD R DSL E R S  LNRNLE  +PILSEE TK L VPSPVK+D+ SSS+
Sbjct: 181 MNSSSPYKDQDLRVDSLFEKRISLSLNRNLEIGNPILSEESTKALTVPSPVKQDVTSSSV 240

Query: 241 AADMEEGSRTKANL-WKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           A D+EEGS  +A+  WKPFKESIGHTE+EVAAGALLGFTVSLITN+ L
Sbjct: 241 ANDLEEGSIKEASSSWKPFKESIGHTEIEVAAGALLGFTVSLITNSFL 271

BLAST of MC02g0659 vs. ExPASy TrEMBL
Match: A0A6J1G8I3 (uncharacterized protein LOC111451718 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451718 PE=4 SV=1)

HSP 1 Score: 356 bits (914), Expect = 3.62e-121
Identity = 201/289 (69.55%), Postives = 221/289 (76.47%), Query Frame = 0

Query: 1   MSLPWWSSATAVPFPSQPRLITHHRH---YSHIHLRKLCPSPSNFTAPRAVLLDEIVQLS 60
           MSLPWW+SATA+PFP   RLI HH +   +S+I L K   SPS  TAPRA LLDE +QLS
Sbjct: 1   MSLPWWTSATALPFPPHTRLINHHCNRLLHSYIRLAKSSSSPSKSTAPRAQLLDEFIQLS 60

Query: 61  HNKVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAE 120
           HNKVLVAAGVSAAIGQLAKPF SV+ YGR+FNFRTAF AGGFPSTHSSAVVAAATI+G E
Sbjct: 61  HNKVLVAAGVSAAIGQLAKPFTSVLFYGREFNFRTAFGAGGFPSTHSSAVVAAATIIGVE 120

Query: 121 RGLADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYFLFGPLRREVGNHAKAINKLLQTK 180
           RGLADSIFG+TV+YASLIMYDAQ                  +RREVG HAKAIN LLQTK
Sbjct: 121 RGLADSIFGITVIYASLIMYDAQG-----------------VRREVGKHAKAINTLLQTK 180

Query: 181 TQVTSSFPCKDQDHRADSLLENRTSPLNRNLESSSPILSEEGTKTLKVPSPVKE-DIASS 240
           T V SSF  +DQD R DS            LESSSP+LSEEGT+ L V SP+KE DIASS
Sbjct: 181 TPVNSSFSYEDQDRRVDS-----------QLESSSPLLSEEGTRPLTVLSPLKEEDIASS 240

Query: 241 SLAADMEEGSRTKANLWKPFKESIGHTEVEVAAGALLGFTVSLITNTLL 285
           SLA D+EEGSR + + WK FKESIGHTE+EVAAGALLGFTVSLITNTLL
Sbjct: 241 SLATDVEEGSRREGSEWKSFKESIGHTEIEVAAGALLGFTVSLITNTLL 261

BLAST of MC02g0659 vs. TAIR 10
Match: AT3G12685.1 (Acid phosphatase/vanadium-dependent haloperoxidase-related protein )

HSP 1 Score: 164.1 bits (414), Expect = 1.7e-40
Identity = 110/251 (43.82%), Postives = 135/251 (53.78%), Query Frame = 0

Query: 36  CP-SPSNFTAPRAVLLDEIVQLSHNKVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAF 95
           CP  P   T    V   +I ++ HNKVL+AAG SA IGQL+KPF SV++YG++ +FR+ F
Sbjct: 29  CPKKPPRLTCVVNVGFQDIAEVIHNKVLIAAGTSAVIGQLSKPFTSVVLYGKNLDFRSVF 88

Query: 96  EAGGFPSTHSSAVVAAATILGAERGLADSIFGLTVVYASLIMYDAQASMKGLLLLFLFYF 155
           +AGGFPSTHSS+VVAAAT +  ERG ADSIFGLTVVYA LIMYDAQ              
Sbjct: 89  QAGGFPSTHSSSVVAAATAIAFERGFADSIFGLTVVYAGLIMYDAQG------------- 148

Query: 156 LFGPLRREVGNHAKAINKLLQTKTQVTSSFPCKDQDHRADSLLENRTSPLNRNLESSSPI 215
               +RREVG HAK +NKL     +                   +    L  N ES+  +
Sbjct: 149 ----VRREVGKHAKVLNKLTANARR-------------------SEVMSLKGN-ESNKAL 208

Query: 216 LSEEGTKTLKVPSPVKEDIASSSLAADMEEGSRTKANLWKPFKESIGHTEVEVAAGALLG 275
            SEE          + E+IA                    P KESIGHTEVEV AGAL G
Sbjct: 209 TSEE----------ISEEIA-------------------PPLKESIGHTEVEVIAGALFG 213

Query: 276 FTVSLITNTLL 286
           F V+L   +L+
Sbjct: 269 FLVTLSVYSLM 213

BLAST of MC02g0659 vs. TAIR 10
Match: AT1G24350.1 (Acid phosphatase/vanadium-dependent haloperoxidase-related protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.0e-05
Identity = 29/81 (35.80%), Postives = 44/81 (54.32%), Query Frame = 0

Query: 59  NKVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAER 118
           N  L++A  S  I Q  K F S     R ++ +    +GG PS+HS+ V A A  +G + 
Sbjct: 22  NYPLISAVTSFTIAQFIKLFTS-WYRERRWDLKQLIGSGGMPSSHSATVTALAVAIGLQE 81

Query: 119 GLADSIFGLTVVYASLIMYDA 140
           G   S F + ++ AS++MYDA
Sbjct: 82  GFGGSHFAIALILASVVMYDA 101

BLAST of MC02g0659 vs. TAIR 10
Match: AT1G24350.2 (Acid phosphatase/vanadium-dependent haloperoxidase-related protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.0e-05
Identity = 29/81 (35.80%), Postives = 44/81 (54.32%), Query Frame = 0

Query: 59  NKVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAER 118
           N  L++A  S  I Q  K F S     R ++ +    +GG PS+HS+ V A A  +G + 
Sbjct: 22  NYPLISAVTSFTIAQFIKLFTS-WYRERRWDLKQLIGSGGMPSSHSATVTALAVAIGLQE 81

Query: 119 GLADSIFGLTVVYASLIMYDA 140
           G   S F + ++ AS++MYDA
Sbjct: 82  GFGGSHFAIALILASVVMYDA 101

BLAST of MC02g0659 vs. TAIR 10
Match: AT1G24350.3 (Acid phosphatase/vanadium-dependent haloperoxidase-related protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.0e-05
Identity = 29/81 (35.80%), Postives = 44/81 (54.32%), Query Frame = 0

Query: 59  NKVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAATILGAER 118
           N  L++A  S  I Q  K F S     R ++ +    +GG PS+HS+ V A A  +G + 
Sbjct: 22  NYPLISAVTSFTIAQFIKLFTS-WYRERRWDLKQLIGSGGMPSSHSATVTALAVAIGLQE 81

Query: 119 GLADSIFGLTVVYASLIMYDA 140
           G   S F + ++ AS++MYDA
Sbjct: 82  GFGGSHFAIALILASVVMYDA 101

BLAST of MC02g0659 vs. TAIR 10
Match: AT3G61770.1 (Acid phosphatase/vanadium-dependent haloperoxidase-related protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.7e-05
Identity = 34/109 (31.19%), Postives = 58/109 (53.21%), Query Frame = 0

Query: 53  IVQLSHNKVLVAAGVSAAIGQLAKPFASVIIYGRDFNFRTAFEAGGFPSTHSSAVVAAAT 112
           +  LS N   V+A V+    Q +K   +  I  R ++FR  + +GG PS+HS+  +A  T
Sbjct: 134 VATLSANPTFVSAVVAWFFAQSSKMVINFFI-ERKWDFRLLYASGGMPSSHSALCMALTT 193

Query: 113 ILGAERGLADSIFGLTVVYASLIMYDA---------QASMKGLLLLFLF 153
            +    G+ADS+F + + ++ ++MYDA         QA +  L++  LF
Sbjct: 194 SVALCHGVADSLFPVCLGFSLIVMYDAIGVRRHAGMQAEVLNLIIRDLF 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O321076.5e-0532.04Uncharacterized membrane protein YuiD OS=Bacillus subtilis (strain 168) OX=22430... [more]
Match NameE-valueIdentityDescription
XP_022149578.19.37e-17992.98uncharacterized protein LOC111017975 [Momordica charantia][more]
XP_038901649.16.76e-13675.09uncharacterized protein LOC120088431 [Benincasa hispida][more]
KAA0033550.17.82e-13274.31Acid phosphatase/vanadium-dependent haloperoxidase-related protein [Cucumis melo... [more]
KAE8646867.13.40e-13172.92hypothetical protein Csa_021036 [Cucumis sativus][more]
XP_004139920.11.96e-13072.57uncharacterized protein LOC101219161 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1D7624.54e-17992.98uncharacterized protein LOC111017975 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A5A7SRL43.79e-13274.31Acid phosphatase/vanadium-dependent haloperoxidase-related protein OS=Cucumis me... [more]
A0A0A0KB689.48e-13172.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G134360 PE=4 SV=1[more]
A0A1S3C2U06.34e-12972.57uncharacterized protein LOC103496264 OS=Cucumis melo OX=3656 GN=LOC103496264 PE=... [more]
A0A6J1G8I33.62e-12169.55uncharacterized protein LOC111451718 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT3G12685.11.7e-4043.82Acid phosphatase/vanadium-dependent haloperoxidase-related protein [more]
AT1G24350.11.0e-0535.80Acid phosphatase/vanadium-dependent haloperoxidase-related protein [more]
AT1G24350.21.0e-0535.80Acid phosphatase/vanadium-dependent haloperoxidase-related protein [more]
AT1G24350.31.0e-0535.80Acid phosphatase/vanadium-dependent haloperoxidase-related protein [more]
AT3G61770.11.7e-0531.19Acid phosphatase/vanadium-dependent haloperoxidase-related protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003832Protein of unknown function DUF212PFAMPF02681DUF212coord: 58..140
e-value: 1.9E-18
score: 66.9
IPR003832Protein of unknown function DUF212PANTHERPTHR31446ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEINcoord: 27..140
IPR003832Protein of unknown function DUF212PANTHERPTHR31446ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEINcoord: 159..284
NoneNo IPR availablePANTHERPTHR31446:SF2ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEINcoord: 27..140
coord: 159..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0659.1MC02g0659.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
molecular_function GO:0004601 peroxidase activity