MC02g0615 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0615
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionWRKY domain-containing protein
LocationMC02: 4928813 .. 4930767 (+)
RNA-Seq ExpressionMC02g0615
SyntenyMC02g0615
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCATTCAATTTAAGACACACATCTCTCAAATTGATCCTCTGTGTGCATAATAGGACAAAGATCTAAGGCAAAGTTTCACTTTTTTACACACCCAAAGGAGAAAATAAGAAGCAAAAGGATCTAAAAATTCAGCCTTCAGCTTTTTTCTTTCTGCTCTTGCTCACTGAGCTGGTTCTCTTCTTCTTCTTTCATTTTTGTCAATGTCAAAGGAAGATGAGAAAAAGAAGGATCCTTATTATTATGATCACCACTTTGATCCTCCTTATGGTGCTGATTTTGATCCCTCCTCTGTTGTTGGTTTCTCTGATTTCTTACATGACTACAACTTCAACTTCTTCTCCGCCGCACTCGACATGTCGTGCTCGTCGTCCGAAGTTATTTCTCCGGTCGATGACGCTTCGAAGAAGTCTTCCGGGGTAGGAGCTGAGTGGGCGGTGACCGGTGAGCACCCATCGTCGTCGTGTTCGTCCGATGATCGGGTGGCGGCTGGGGGTGCTGGTGGTGGTGATTCTGCCAAGAGTGGAGATCAGGTGGTGACGAAAGAGTTTGAAGATAAAGATGGAGAAAAGTGTAAGAAGAAAGTGTAGGTCACTCTTCTCTTAAAAATCTCTTGAATTTTTATAATTTTTTTTTAGGTTTGACAAAAACTAACAAGAGAAATCAGGAAAATTAGGGTTCTTGTGGCCTTTTAAAGTCTAAATTCAACAACCCACTTGTTGTTTTTAACTAAAAAGGAGAGAAATTGATCTAAATTGATGATTAACACACACCAGATTGTAATTTGTGAGCTAAATTATATACATTTACAAGAAAAAAGGAATTGAGAGAAAAAATTGGTGTATTGTGTATAGGGAAGGAGGAAAAAAGAAGGAGAAAGGGGAGAAGGGGCCACGGGTTGCGTTCTTGACCAAGACTGAAATAGATAACCTTGAAGATGGATACCGATGGAGAAAATATGGTCAGAAAGCTGTCAAAAACAGCCCTTTTCCAAGGTATAATTTTCCTACACTCTTACCTTAATTCAATCAAATTAAATTGGGTATTTTTAATTAAATTTCTCACTACTACTAAGTTTATTGAAATAATAAGATTATACATCTTGAAGTCATTTAGTTTAACACCTAATTCATGAGTTGAATTGTAAGTAACAATCTGATTGTAACCCTAGAAATATATTCCTATTGTTTTTGTACTCCCTTTTTCATGAATTGTGCCTCTTAAGTTGTGTTTGGGAGACTTGTGGAGTTTTGAGTTTTAGGAAACATTGATTCATGAAAATTATGTGAGAGAATAGGGTTTCGAAAGAGTACTAGATTTAGCGTAAACTCGTTAGTTTATCCAACAAATCGGATCGAGGGGACAAGCAATAGTTTTGCCTCTACGACCTTCTTAGTCATGTCACTTTCACTCGGAATTTAAATAATCAGATTATTACCAAATCTAAAAGCTTAAATAGATTGTGGTTTTTATTTTGTTTTTCTTACAGGAGTTACTACAAATGCACAACTCAAAACTGCAATGTCAAGAAAAGAGTTGAGAGATCATCCCAAGATCCATCTTTTGTAGTAACAACGTACGAAGGGAAACACAACCACTATTGTCCCGTGACACTTCGAGGCAATGTCGCTGGAATGTCGTTGCCGTTGTCGTCTGCTTCACCGCCGTGGCCTCCGGCCAACTTCTTCTCCGGCGATCCTTATAAAGGAGTTGTTGATGATCACCAAGATTATTCTCAGGTTATTCCTGATCATTATGGGTTGTTGCAAGATTTGATTCCATCCTCGTTCAATCGAAAGCAAGAGCCTTGAGTTACAAAATCTTCTCTCTTAGTTATACATATTATGAACTTTATATTAATTAGCTAGTGCACATCTTGCATTTAATCTTCTTGATTAGCTAGTCAATACTCTAATTTTGTTGAGAGTGCTGTTAACTAATTTAGATCAATTTCAT

mRNA sequence

CACCATTCAATTTAAGACACACATCTCTCAAATTGATCCTCTGTGTGCATAATAGGACAAAGATCTAAGGCAAAGTTTCACTTTTTTACACACCCAAAGGAGAAAATAAGAAGCAAAAGGATCTAAAAATTCAGCCTTCAGCTTTTTTCTTTCTGCTCTTGCTCACTGAGCTGGTTCTCTTCTTCTTCTTTCATTTTTGTCAATGTCAAAGGAAGATGAGAAAAAGAAGGATCCTTATTATTATGATCACCACTTTGATCCTCCTTATGGTGCTGATTTTGATCCCTCCTCTGTTGTTGGTTTCTCTGATTTCTTACATGACTACAACTTCAACTTCTTCTCCGCCGCACTCGACATGTCGTGCTCGTCGTCCGAAGTTATTTCTCCGGTCGATGACGCTTCGAAGAAGTCTTCCGGGGTAGGAGCTGAGTGGGCGGTGACCGGTGAGCACCCATCGTCGTCGTGTTCGTCCGATGATCGGGTGGCGGCTGGGGGTGCTGGTGGTGGTGATTCTGCCAAGAGTGGAGATCAGGTGGTGACGAAAGAGTTTGAAGATAAAGATGGAGAAAAGTGTAAGAAGAAAGTGGAAGGAGGAAAAAAGAAGGAGAAAGGGGAGAAGGGGCCACGGGTTGCGTTCTTGACCAAGACTGAAATAGATAACCTTGAAGATGGATACCGATGGAGAAAATATGGTCAGAAAGCTGTCAAAAACAGCCCTTTTCCAAGGAGTTACTACAAATGCACAACTCAAAACTGCAATGTCAAGAAAAGAGTTGAGAGATCATCCCAAGATCCATCTTTTGTAGTAACAACGTACGAAGGGAAACACAACCACTATTGTCCCGTGACACTTCGAGGCAATGTCGCTGGAATGTCGTTGCCGTTGTCGTCTGCTTCACCGCCGTGGCCTCCGGCCAACTTCTTCTCCGGCGATCCTTATAAAGGAGTTGTTGATGATCACCAAGATTATTCTCAGGTTATTCCTGATCATTATGGGTTGTTGCAAGATTTGATTCCATCCTCGTTCAATCGAAAGCAAGAGCCTTGAGTTACAAAATCTTCTCTCTTAGTTATACATATTATGAACTTTATATTAATTAGCTAGTGCACATCTTGCATTTAATCTTCTTGATTAGCTAGTCAATACTCTAATTTTGTTGAGAGTGCTGTTAACTAATTTAGATCAATTTCAT

Coding sequence (CDS)

ATGTCAAAGGAAGATGAGAAAAAGAAGGATCCTTATTATTATGATCACCACTTTGATCCTCCTTATGGTGCTGATTTTGATCCCTCCTCTGTTGTTGGTTTCTCTGATTTCTTACATGACTACAACTTCAACTTCTTCTCCGCCGCACTCGACATGTCGTGCTCGTCGTCCGAAGTTATTTCTCCGGTCGATGACGCTTCGAAGAAGTCTTCCGGGGTAGGAGCTGAGTGGGCGGTGACCGGTGAGCACCCATCGTCGTCGTGTTCGTCCGATGATCGGGTGGCGGCTGGGGGTGCTGGTGGTGGTGATTCTGCCAAGAGTGGAGATCAGGTGGTGACGAAAGAGTTTGAAGATAAAGATGGAGAAAAGTGTAAGAAGAAAGTGGAAGGAGGAAAAAAGAAGGAGAAAGGGGAGAAGGGGCCACGGGTTGCGTTCTTGACCAAGACTGAAATAGATAACCTTGAAGATGGATACCGATGGAGAAAATATGGTCAGAAAGCTGTCAAAAACAGCCCTTTTCCAAGGAGTTACTACAAATGCACAACTCAAAACTGCAATGTCAAGAAAAGAGTTGAGAGATCATCCCAAGATCCATCTTTTGTAGTAACAACGTACGAAGGGAAACACAACCACTATTGTCCCGTGACACTTCGAGGCAATGTCGCTGGAATGTCGTTGCCGTTGTCGTCTGCTTCACCGCCGTGGCCTCCGGCCAACTTCTTCTCCGGCGATCCTTATAAAGGAGTTGTTGATGATCACCAAGATTATTCTCAGGTTATTCCTGATCATTATGGGTTGTTGCAAGATTTGATTCCATCCTCGTTCAATCGAAAGCAAGAGCCTTGA

Protein sequence

MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVISPVDDASKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANFFSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP
Homology
BLAST of MC02g0615 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.6e-38
Identity = 104/226 (46.02%), Postives = 136/226 (60.18%), Query Frame = 0

Query: 83  HPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKD------GEKCKKKVEGGKKK-E 142
           +P  + +S+  V +  +  G+  K      + + ED +      GE  K+  + GKKK E
Sbjct: 58  NPYLNLTSNSPVVSSSSNEGE-PKENTNDKSDQMEDNEGDLHGVGESSKQLTKQGKKKGE 117

Query: 143 KGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSS 202
           K E+  RVAF+TK+EID+LEDGYRWRKYGQKAVKNSP+PRSYY+CTTQ CNVKKRVERS 
Sbjct: 118 KKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSF 177

Query: 203 QDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPL-----SSASPPWP------------PA 262
           QDPS V+TTYEGKHNH  P TLRG VA   L +      S    +P            PA
Sbjct: 178 QDPSIVITTYEGKHNHPIPSTLRGTVAAEHLLVHRGGGGSLLHSFPRHHQDFLMMKHSPA 237

Query: 263 NF-----FSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQ 280
           N+      S +   G    + + +Q + D YGLLQD++PS F++ +
Sbjct: 238 NYQSVGSLSYEHGHGTSSYNFNNNQPVVD-YGLLQDIVPSMFSKNE 281

BLAST of MC02g0615 vs. ExPASy Swiss-Prot
Match: Q9FL26 (WRKY transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=WRKY8 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.7e-34
Identity = 120/296 (40.54%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 22  YGADFDPSSVVGFSDFLHDYNFN---FFSAALDMSCSSSEVISPVDD------------A 81
           Y A+   SS VGF   L   N +    F    ++S SSS+  +P  D            +
Sbjct: 39  YNANMISSSQVGFD--LPSKNLSPQGAFELGFELSPSSSDFFNPSLDQENGLYNAYNYNS 98

Query: 82  SKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDG---EK 141
           S+KS  V  +   T +      +S     A    G DS K     + K+ E +DG   ++
Sbjct: 99  SQKSHEVVGDGCATIKSEVRVSASPSSSEADHHPGEDSGK-----IRKKREVRDGGEDDQ 158

Query: 142 CKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQ 201
             +KV   KKKE+ +K PRV+F+TKTE+D+LEDGYRWRKYGQKAVKNSP+PRSYY+CTTQ
Sbjct: 159 RSQKVVKTKKKEEKKKEPRVSFMTKTEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQ 218

Query: 202 NCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLR-----GNVAG----MSLPLSSASPP 261
            CNVKKRVERS QDP+ V+TTYE +HNH  P   R     G  A      S P+ S    
Sbjct: 219 KCNVKKRVERSYQDPTVVITTYESQHNHPIPTNRRTAMFSGTTASDYNPSSSPIFSDLII 278

Query: 262 WPPANFFSGD----PYKGVVDDHQDYSQVIPDH-----YGLLQDLIPSSFNRKQEP 282
             P +F + D    PY  V  +   + Q    H     + LL+++ PS F  KQEP
Sbjct: 279 NTPRSFSNDDLFRVPYASVNVNPSYHQQQHGFHQQESEFELLKEMFPSVF-FKQEP 326

BLAST of MC02g0615 vs. ExPASy Swiss-Prot
Match: Q8VWJ2 (WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.5e-34
Identity = 115/289 (39.79%), Postives = 154/289 (53.29%), Query Frame = 0

Query: 26  FDPSSVVGFSDFLHDYNFNFFS---AALDMSCSSSEVI-SPVDDASKKS------SGVGA 85
           F+PS+   F+D L      + S       +S SSSEV  S +D    +       +G   
Sbjct: 42  FNPST-YSFTDCLQSSPAAYESLLQKTFGLSPSSSEVFNSSIDQEPNRDVTNDVINGGAC 101

Query: 86  EWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKE 145
               T   PS+S SS+         G DS KS  +   +E   ++ +  KK  +  K + 
Sbjct: 102 NETETRVSPSNSSSSE-----ADHPGEDSGKSRRK---RELVGEEDQISKKVGKTKKTEV 161

Query: 146 KGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSS 205
           K ++ PRV+F+TK+E+D+LEDGYRWRKYGQKAVKNSP+PRSYY+CTTQ CNVKKRVERS 
Sbjct: 162 KKQREPRVSFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSF 221

Query: 206 QDPSFVVTTYEGKHNHYCPVTLRGNVAGMSL-------PLSSASPPWPPANFFSGDPYKG 265
           QDP+ V+TTYEG+HNH  P  LRG+ A  ++       P S A   +  A + +G     
Sbjct: 222 QDPTVVITTYEGQHNHPIPTNLRGSSAAAAMFSADLMTPRSFAHDMFRTAAYTNGGSVAA 281

Query: 266 VVD----------------DHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 282
            +D                 HQ Y Q     Y LL+++ PS F  KQEP
Sbjct: 282 ALDYGYGQSGYGSVNSNPSSHQVYHQ--GGEYELLREIFPSIF-FKQEP 318

BLAST of MC02g0615 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.6e-32
Identity = 82/174 (47.13%), Postives = 108/174 (62.07%), Query Frame = 0

Query: 85  SSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKEKGEKGPRVA 144
           S+S SS    AA     G      DQ    + +++ G K + K +  KK +K  +  R A
Sbjct: 153 STSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQLKAK--KKNQKKAREARFA 212

Query: 145 FLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSSQDPSFVVTT 204
           FLTK++IDNL+DGYRWRKYGQKAVKNSP+PRSYY+CTT  C VKKRVERSS DPS V+TT
Sbjct: 213 FLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDDPSIVMTT 272

Query: 205 YEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANFFS-GDPYKGVVDDHQDYS 258
           YEG+H H  P+T RG++  ++ P+         ++ FS   P   +   HQ Y+
Sbjct: 273 YEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQPRYLLTQHHQPYN 324

BLAST of MC02g0615 vs. ExPASy Swiss-Prot
Match: O22900 (WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.7e-32
Identity = 85/197 (43.15%), Postives = 114/197 (57.87%), Query Frame = 0

Query: 84  PSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKEKGEKGPRV 143
           P++  SS    A+  A   +  K+ D       + ++    KK+++  K  +K ++  RV
Sbjct: 103 PATPNSSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQKRQREARV 162

Query: 144 AFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSSQDPSFVVT 203
           AF+TK+E+D+LEDGYRWRKYGQKAVKNSPFPRSYY+CTT +CNVKKRVERS +DPS VVT
Sbjct: 163 AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRDPSTVVT 222

Query: 204 TYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANFFSGDPYKGVVDDHQDYSQVIPDH 263
           TYEG+H H  P+T R    G     S A+      N   G P  G       + Q++  H
Sbjct: 223 TYEGQHTHISPLTSRPISTGGFFGSSGAASSL--GNGCFGFPIDGSTLISPQFQQLVQYH 282

Query: 264 YGLLQDLIPSSFNRKQE 281
           +   Q  + S F    E
Sbjct: 283 HQQQQQELMSCFGGVNE 297

BLAST of MC02g0615 vs. NCBI nr
Match: XP_022149678.1 (probable WRKY transcription factor 50 [Momordica charantia])

HSP 1 Score: 574 bits (1480), Expect = 8.10e-207
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 1   MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI 60
           MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI
Sbjct: 1   MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI 60

Query: 61  SPVDDASKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKD 120
           SPVDDASKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKD
Sbjct: 61  SPVDDASKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKD 120

Query: 121 GEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKC 180
           GEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKC
Sbjct: 121 GEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKC 180

Query: 181 TTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANF 240
           TTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANF
Sbjct: 181 TTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANF 240

Query: 241 FSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 281
           FSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP
Sbjct: 241 FSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 281

BLAST of MC02g0615 vs. NCBI nr
Match: XP_038902581.1 (probable WRKY transcription factor 56 [Benincasa hispida])

HSP 1 Score: 304 bits (778), Expect = 1.34e-99
Identity = 184/316 (58.23%), Postives = 221/316 (69.94%), Query Frame = 0

Query: 1   MSKED----EKKKDPYY-----YDHHFDP-----------PYGADFD--PSSVVGFSDFL 60
           MS ED    E++++PYY     + H+F P           PY   FD  PSSV  +SDFL
Sbjct: 1   MSNEDHHHHEEEENPYYPNFDPFTHNFHPHKPFEAPPLTSPYYEAFDIIPSSVGFYSDFL 60

Query: 61  H---DYNFNFFSAALDMSCSSSEVISPVDDASKKSSGVGAEWAVTGEHPSS------SCS 120
           H   +Y +N  +  L+MSCSSSEVISP+DDASKKS G+  +  VTGEHPS+      +CS
Sbjct: 61  HVSAEYGYN--NNLLEMSCSSSEVISPIDDASKKSLGLRDQSVVTGEHPSTPNCSSTTCS 120

Query: 121 SDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKEKGEKGPRVAFLTKT 180
           SD+      AGGG+S+KSG+    K F+DK+GE  KK V+GGKKKEK EKGPR AF+TKT
Sbjct: 121 SDEAA----AGGGESSKSGE---VKGFDDKNGENSKK-VDGGKKKEKREKGPRFAFMTKT 180

Query: 181 EIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSSQDPSFVVTTYEGKH 240
           EIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCT+QNC+VKKRVERSS+DP FV+TTYEGKH
Sbjct: 181 EIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPCFVITTYEGKH 240

Query: 241 NHYCPVTLRGNVAGMSLPLSSASPPWP---PANFFSGDPYKGVVDDHQDYSQVIPDHYGL 281
           NHYCP+ LRG+ A   LP S + P  P   PANF SG+ Y+ +  + Q Y   +PD YGL
Sbjct: 241 NHYCPIILRGHNATGVLPPSVSPPYLPGLAPANFLSGEFYENL--NQQQYYDQVPDQYGL 300

BLAST of MC02g0615 vs. NCBI nr
Match: XP_022943476.1 (probable WRKY transcription factor 28 isoform X1 [Cucurbita moschata])

HSP 1 Score: 279 bits (714), Expect = 1.02e-90
Identity = 171/287 (59.58%), Postives = 200/287 (69.69%), Query Frame = 0

Query: 1   MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI 60
           MS  ++ +KDPYY      P + A FDPS+V  +SDFL+DYN NF S  +D+SCSSSE I
Sbjct: 1   MSNNEDHEKDPYY------PNFEA-FDPSNVGFYSDFLYDYN-NFLSTDIDISCSSSEAI 60

Query: 61  SPVDDASKKSSGVGAEWAVTGEHPSS-----SCSSDDRVAAGGAGGGDSAKSGDQVVTKE 120
           SP++DA KKS G+G E   TGEHPS+     +CSS +      AGG DSAKSG+      
Sbjct: 61  SPINDALKKSLGLG-ESVATGEHPSTPNSSTTCSSVEAA----AGGRDSAKSGEV----- 120

Query: 121 FEDKDGEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPR 180
                      KV+G KKKEK EKGPR AFLTK+EIDNLEDGYRWRKYGQKAVKNSPFPR
Sbjct: 121 -----------KVDGRKKKEKREKGPRFAFLTKSEIDNLEDGYRWRKYGQKAVKNSPFPR 180

Query: 181 SYYKCTTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRG-NVAGMSLPLSSASPP 240
           SYYKCT+QNC+VKKRVERSS+DP FVVTTYEGKHNHYCP+TLRG NVAG+  P  SASP 
Sbjct: 181 SYYKCTSQNCSVKKRVERSSEDPCFVVTTYEGKHNHYCPITLRGHNVAGVYPP--SASPS 240

Query: 241 WPPANFFSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 281
             PA   +G+ Y GV D  Q Y +V    YG+L+DL+    N KQ P
Sbjct: 241 LIPARILAGEFYGGV-DVQQQYYEV--PGYGVLEDLV----NPKQNP 249

BLAST of MC02g0615 vs. NCBI nr
Match: XP_023512278.1 (probable WRKY transcription factor 48 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 278 bits (712), Expect = 2.05e-90
Identity = 170/287 (59.23%), Postives = 200/287 (69.69%), Query Frame = 0

Query: 1   MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI 60
           MS  ++ +KDPYY      P + A FDPS+V  +SDFL+DYN NF S  +D+SCSSSE I
Sbjct: 1   MSNNEDHEKDPYY------PNFEA-FDPSNVGFYSDFLYDYN-NFLSTDIDISCSSSEAI 60

Query: 61  SPVDDASKKSSGVGAEWAVTGEHPSS-----SCSSDDRVAAGGAGGGDSAKSGDQVVTKE 120
           SP++DA KKS G+G   A+ GEHPS+     +CSS +      AGG DSAKSG+      
Sbjct: 61  SPINDALKKSLGLGESVAI-GEHPSTPNSSTTCSSVEAA----AGGSDSAKSGEV----- 120

Query: 121 FEDKDGEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPR 180
                      K++G KKKEK EKGPR AFLTK+EIDNLEDGYRWRKYGQKAVKNSPFPR
Sbjct: 121 -----------KLDGRKKKEKREKGPRFAFLTKSEIDNLEDGYRWRKYGQKAVKNSPFPR 180

Query: 181 SYYKCTTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRG-NVAGMSLPLSSASPP 240
           SYYKCT+QNC+VKKRVERSS+DP FVVTTYEGKHNHYCP+TLRG NVAG+  P  SASP 
Sbjct: 181 SYYKCTSQNCSVKKRVERSSEDPCFVVTTYEGKHNHYCPITLRGHNVAGVYPP--SASPS 240

Query: 241 WPPANFFSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 281
             PA   SG+ Y GV D  Q Y +V    YG+L+DL+    N KQ P
Sbjct: 241 LIPARILSGEFYGGV-DVQQQYYEV--PGYGVLEDLV----NPKQNP 249

BLAST of MC02g0615 vs. NCBI nr
Match: XP_011656990.1 (probable WRKY transcription factor 12 isoform X1 [Cucumis sativus] >KGN46828.1 hypothetical protein Csa_020630 [Cucumis sativus])

HSP 1 Score: 276 bits (707), Expect = 7.66e-89
Identity = 171/279 (61.29%), Postives = 197/279 (70.61%), Query Frame = 0

Query: 21  PYGADFD--PSSVVGFSDFLH-----DYNFNFFSAALDMSCSSSEVISPVDDASKKSSGV 80
           PY   FD  PSSV  +SDF H     DY+ N FS  ++MSCSSSEVIS VDDA KKSS +
Sbjct: 42  PYYEAFDIAPSSVGFYSDFSHASSQYDYH-NIFSTPIEMSCSSSEVISSVDDALKKSSSL 101

Query: 81  GAEWA--VTGEHPSS------SCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCK 140
           G + +  VTGEHPS+      +CSSD+ V    AGGGDS+KSG+    K F+DK GE  K
Sbjct: 102 GRDLSSVVTGEHPSTPNCSSTTCSSDEVV----AGGGDSSKSGE---VKGFDDKKGENSK 161

Query: 141 KKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNC 200
           K V+ GKKKEK EKGPR AFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCT+QNC
Sbjct: 162 K-VDEGKKKEKREKGPRFAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTSQNC 221

Query: 201 NVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPA-NFFSGD 260
           +VKKRVERSS+DPSFV+TTYEGKHNHYCP+TLRG+     LP S   P +PP+ NFFS +
Sbjct: 222 SVKKRVERSSEDPSFVITTYEGKHNHYCPITLRGHNPTGVLPPSVTPPLFPPSPNFFSAE 281

Query: 261 PYKGVVDDHQDYSQVIPDHYGLLQDLI--PSSFNRKQEP 281
            +   +  HQ Y      + GL QD I  PS  N  Q P
Sbjct: 282 SFCENL--HQQYQ-----YGGLFQDFINNPSFNNSNQHP 304

BLAST of MC02g0615 vs. ExPASy TrEMBL
Match: A0A6J1D7F3 (probable WRKY transcription factor 50 OS=Momordica charantia OX=3673 GN=LOC111018047 PE=4 SV=1)

HSP 1 Score: 574 bits (1480), Expect = 3.92e-207
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 1   MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI 60
           MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI
Sbjct: 1   MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI 60

Query: 61  SPVDDASKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKD 120
           SPVDDASKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKD
Sbjct: 61  SPVDDASKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKD 120

Query: 121 GEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKC 180
           GEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKC
Sbjct: 121 GEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKC 180

Query: 181 TTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANF 240
           TTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANF
Sbjct: 181 TTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANF 240

Query: 241 FSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 281
           FSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP
Sbjct: 241 FSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 281

BLAST of MC02g0615 vs. ExPASy TrEMBL
Match: A0A6J1FRU0 (probable WRKY transcription factor 28 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448234 PE=4 SV=1)

HSP 1 Score: 279 bits (714), Expect = 4.94e-91
Identity = 171/287 (59.58%), Postives = 200/287 (69.69%), Query Frame = 0

Query: 1   MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI 60
           MS  ++ +KDPYY      P + A FDPS+V  +SDFL+DYN NF S  +D+SCSSSE I
Sbjct: 1   MSNNEDHEKDPYY------PNFEA-FDPSNVGFYSDFLYDYN-NFLSTDIDISCSSSEAI 60

Query: 61  SPVDDASKKSSGVGAEWAVTGEHPSS-----SCSSDDRVAAGGAGGGDSAKSGDQVVTKE 120
           SP++DA KKS G+G E   TGEHPS+     +CSS +      AGG DSAKSG+      
Sbjct: 61  SPINDALKKSLGLG-ESVATGEHPSTPNSSTTCSSVEAA----AGGRDSAKSGEV----- 120

Query: 121 FEDKDGEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPR 180
                      KV+G KKKEK EKGPR AFLTK+EIDNLEDGYRWRKYGQKAVKNSPFPR
Sbjct: 121 -----------KVDGRKKKEKREKGPRFAFLTKSEIDNLEDGYRWRKYGQKAVKNSPFPR 180

Query: 181 SYYKCTTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRG-NVAGMSLPLSSASPP 240
           SYYKCT+QNC+VKKRVERSS+DP FVVTTYEGKHNHYCP+TLRG NVAG+  P  SASP 
Sbjct: 181 SYYKCTSQNCSVKKRVERSSEDPCFVVTTYEGKHNHYCPITLRGHNVAGVYPP--SASPS 240

Query: 241 WPPANFFSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 281
             PA   +G+ Y GV D  Q Y +V    YG+L+DL+    N KQ P
Sbjct: 241 LIPARILAGEFYGGV-DVQQQYYEV--PGYGVLEDLV----NPKQNP 249

BLAST of MC02g0615 vs. ExPASy TrEMBL
Match: A0A0A0KBC5 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139770 PE=4 SV=1)

HSP 1 Score: 276 bits (707), Expect = 3.71e-89
Identity = 171/279 (61.29%), Postives = 197/279 (70.61%), Query Frame = 0

Query: 21  PYGADFD--PSSVVGFSDFLH-----DYNFNFFSAALDMSCSSSEVISPVDDASKKSSGV 80
           PY   FD  PSSV  +SDF H     DY+ N FS  ++MSCSSSEVIS VDDA KKSS +
Sbjct: 42  PYYEAFDIAPSSVGFYSDFSHASSQYDYH-NIFSTPIEMSCSSSEVISSVDDALKKSSSL 101

Query: 81  GAEWA--VTGEHPSS------SCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCK 140
           G + +  VTGEHPS+      +CSSD+ V    AGGGDS+KSG+    K F+DK GE  K
Sbjct: 102 GRDLSSVVTGEHPSTPNCSSTTCSSDEVV----AGGGDSSKSGE---VKGFDDKKGENSK 161

Query: 141 KKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNC 200
           K V+ GKKKEK EKGPR AFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCT+QNC
Sbjct: 162 K-VDEGKKKEKREKGPRFAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTSQNC 221

Query: 201 NVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPA-NFFSGD 260
           +VKKRVERSS+DPSFV+TTYEGKHNHYCP+TLRG+     LP S   P +PP+ NFFS +
Sbjct: 222 SVKKRVERSSEDPSFVITTYEGKHNHYCPITLRGHNPTGVLPPSVTPPLFPPSPNFFSAE 281

Query: 261 PYKGVVDDHQDYSQVIPDHYGLLQDLI--PSSFNRKQEP 281
            +   +  HQ Y      + GL QD I  PS  N  Q P
Sbjct: 282 SFCENL--HQQYQ-----YGGLFQDFINNPSFNNSNQHP 304

BLAST of MC02g0615 vs. ExPASy TrEMBL
Match: A0A1S3C4A4 (probable WRKY transcription factor 12 OS=Cucumis melo OX=3656 GN=LOC103496315 PE=4 SV=1)

HSP 1 Score: 275 bits (702), Expect = 2.05e-88
Identity = 169/279 (60.57%), Postives = 194/279 (69.53%), Query Frame = 0

Query: 21  PYGADFD--PSSVVGFSDFLH-----DYNFNFFSAALDMSCSSSEVISPVDDASKKSSGV 80
           PY   FD  P +V  +SDF H     DY+ N FS   +MSCSSSEVIS VDDA KKSS +
Sbjct: 42  PYSEAFDIAPYNVGFYSDFSHASSQYDYH-NIFSTPFEMSCSSSEVISSVDDALKKSSSL 101

Query: 81  GAEWA--VTGEHP------SSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCK 140
           G + +  VTGEHP      S++CSSD+ V    AGGGDS+KSG+    K F+DK GE  K
Sbjct: 102 GRDLSSVVTGEHPLTPNCSSTTCSSDEVV----AGGGDSSKSGE---VKGFDDKKGENSK 161

Query: 141 KKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNC 200
           K V+ GKKKEK EKGPR AFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCT+QNC
Sbjct: 162 K-VDEGKKKEKREKGPRFAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTSQNC 221

Query: 201 NVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPA-NFFSGD 260
           +VKKRVERSS+DP FV+TTYEGKHNHYCP+TLRG+     LP S   P +PP+ NFFS +
Sbjct: 222 SVKKRVERSSEDPGFVITTYEGKHNHYCPITLRGHNPTGVLPPSVTPPLFPPSPNFFSAE 281

Query: 261 PYKGVVDDHQDYSQVIPDHYGLLQDLI--PSSFNRKQEP 281
            +   +  HQ Y       YGL QD I  PS  N  Q P
Sbjct: 282 SFCENL--HQQYQ------YGLFQDFINNPSFNNSNQHP 303

BLAST of MC02g0615 vs. ExPASy TrEMBL
Match: A0A6J1FT54 (probable WRKY transcription factor 48 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448234 PE=4 SV=1)

HSP 1 Score: 247 bits (631), Expect = 1.30e-78
Identity = 161/287 (56.10%), Postives = 189/287 (65.85%), Query Frame = 0

Query: 1   MSKEDEKKKDPYYYDHHFDPPYGADFDPSSVVGFSDFLHDYNFNFFSAALDMSCSSSEVI 60
           MS  ++ +KDPYY      P + A FDPS+V  +SDFL+DYN NF S  +D+SCSSSE I
Sbjct: 1   MSNNEDHEKDPYY------PNFEA-FDPSNVGFYSDFLYDYN-NFLSTDIDISCSSSEAI 60

Query: 61  SPVDDASKKSSGVGAEWAVTGEHPSS-----SCSSDDRVAAGGAGGGDSAKSGDQVVTKE 120
           SP++DA KKS G+G E   TGEHPS+     +CSS +      AGG DSAKSG+      
Sbjct: 61  SPINDALKKSLGLG-ESVATGEHPSTPNSSTTCSSVEAA----AGGRDSAKSGEV----- 120

Query: 121 FEDKDGEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPR 180
                      KV+G KKKEK EKGPR AFLTK+EIDNLEDGYRWRKYGQKAVKNSPFP 
Sbjct: 121 -----------KVDGRKKKEKREKGPRFAFLTKSEIDNLEDGYRWRKYGQKAVKNSPFP- 180

Query: 181 SYYKCTTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRG-NVAGMSLPLSSASPP 240
                     +VKKRVERSS+DP FVVTTYEGKHNHYCP+TLRG NVAG+  P  SASP 
Sbjct: 181 ----------SVKKRVERSSEDPCFVVTTYEGKHNHYCPITLRGHNVAGVYPP--SASPS 238

Query: 241 WPPANFFSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 281
             PA   +G+ Y GV D  Q Y +V    YG+L+DL+    N KQ P
Sbjct: 241 LIPARILAGEFYGGV-DVQQQYYEV--PGYGVLEDLV----NPKQNP 238

BLAST of MC02g0615 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 160.6 bits (405), Expect = 1.8e-39
Identity = 104/226 (46.02%), Postives = 136/226 (60.18%), Query Frame = 0

Query: 83  HPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKD------GEKCKKKVEGGKKK-E 142
           +P  + +S+  V +  +  G+  K      + + ED +      GE  K+  + GKKK E
Sbjct: 58  NPYLNLTSNSPVVSSSSNEGE-PKENTNDKSDQMEDNEGDLHGVGESSKQLTKQGKKKGE 117

Query: 143 KGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSS 202
           K E+  RVAF+TK+EID+LEDGYRWRKYGQKAVKNSP+PRSYY+CTTQ CNVKKRVERS 
Sbjct: 118 KKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSF 177

Query: 203 QDPSFVVTTYEGKHNHYCPVTLRGNVAGMSLPL-----SSASPPWP------------PA 262
           QDPS V+TTYEGKHNH  P TLRG VA   L +      S    +P            PA
Sbjct: 178 QDPSIVITTYEGKHNHPIPSTLRGTVAAEHLLVHRGGGGSLLHSFPRHHQDFLMMKHSPA 237

Query: 263 NF-----FSGDPYKGVVDDHQDYSQVIPDHYGLLQDLIPSSFNRKQ 280
           N+      S +   G    + + +Q + D YGLLQD++PS F++ +
Sbjct: 238 NYQSVGSLSYEHGHGTSSYNFNNNQPVVD-YGLLQDIVPSMFSKNE 281

BLAST of MC02g0615 vs. TAIR 10
Match: AT5G46350.1 (WRKY DNA-binding protein 8 )

HSP 1 Score: 147.9 bits (372), Expect = 1.2e-35
Identity = 120/296 (40.54%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 22  YGADFDPSSVVGFSDFLHDYNFN---FFSAALDMSCSSSEVISPVDD------------A 81
           Y A+   SS VGF   L   N +    F    ++S SSS+  +P  D            +
Sbjct: 39  YNANMISSSQVGFD--LPSKNLSPQGAFELGFELSPSSSDFFNPSLDQENGLYNAYNYNS 98

Query: 82  SKKSSGVGAEWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDG---EK 141
           S+KS  V  +   T +      +S     A    G DS K     + K+ E +DG   ++
Sbjct: 99  SQKSHEVVGDGCATIKSEVRVSASPSSSEADHHPGEDSGK-----IRKKREVRDGGEDDQ 158

Query: 142 CKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQ 201
             +KV   KKKE+ +K PRV+F+TKTE+D+LEDGYRWRKYGQKAVKNSP+PRSYY+CTTQ
Sbjct: 159 RSQKVVKTKKKEEKKKEPRVSFMTKTEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQ 218

Query: 202 NCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLR-----GNVAG----MSLPLSSASPP 261
            CNVKKRVERS QDP+ V+TTYE +HNH  P   R     G  A      S P+ S    
Sbjct: 219 KCNVKKRVERSYQDPTVVITTYESQHNHPIPTNRRTAMFSGTTASDYNPSSSPIFSDLII 278

Query: 262 WPPANFFSGD----PYKGVVDDHQDYSQVIPDH-----YGLLQDLIPSSFNRKQEP 282
             P +F + D    PY  V  +   + Q    H     + LL+++ PS F  KQEP
Sbjct: 279 NTPRSFSNDDLFRVPYASVNVNPSYHQQQHGFHQQESEFELLKEMFPSVF-FKQEP 326

BLAST of MC02g0615 vs. TAIR 10
Match: AT4G18170.1 (WRKY DNA-binding protein 28 )

HSP 1 Score: 146.0 bits (367), Expect = 4.6e-35
Identity = 115/289 (39.79%), Postives = 154/289 (53.29%), Query Frame = 0

Query: 26  FDPSSVVGFSDFLHDYNFNFFS---AALDMSCSSSEVI-SPVDDASKKS------SGVGA 85
           F+PS+   F+D L      + S       +S SSSEV  S +D    +       +G   
Sbjct: 42  FNPST-YSFTDCLQSSPAAYESLLQKTFGLSPSSSEVFNSSIDQEPNRDVTNDVINGGAC 101

Query: 86  EWAVTGEHPSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKE 145
               T   PS+S SS+         G DS KS  +   +E   ++ +  KK  +  K + 
Sbjct: 102 NETETRVSPSNSSSSE-----ADHPGEDSGKSRRK---RELVGEEDQISKKVGKTKKTEV 161

Query: 146 KGEKGPRVAFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSS 205
           K ++ PRV+F+TK+E+D+LEDGYRWRKYGQKAVKNSP+PRSYY+CTTQ CNVKKRVERS 
Sbjct: 162 KKQREPRVSFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSF 221

Query: 206 QDPSFVVTTYEGKHNHYCPVTLRGNVAGMSL-------PLSSASPPWPPANFFSGDPYKG 265
           QDP+ V+TTYEG+HNH  P  LRG+ A  ++       P S A   +  A + +G     
Sbjct: 222 QDPTVVITTYEGQHNHPIPTNLRGSSAAAAMFSADLMTPRSFAHDMFRTAAYTNGGSVAA 281

Query: 266 VVD----------------DHQDYSQVIPDHYGLLQDLIPSSFNRKQEP 282
            +D                 HQ Y Q     Y LL+++ PS F  KQEP
Sbjct: 282 ALDYGYGQSGYGSVNSNPSSHQVYHQ--GGEYELLREIFPSIF-FKQEP 318

BLAST of MC02g0615 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-33
Identity = 82/174 (47.13%), Postives = 108/174 (62.07%), Query Frame = 0

Query: 85  SSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKEKGEKGPRVA 144
           S+S SS    AA     G      DQ    + +++ G K + K +  KK +K  +  R A
Sbjct: 153 STSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQLKAK--KKNQKKAREARFA 212

Query: 145 FLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSSQDPSFVVTT 204
           FLTK++IDNL+DGYRWRKYGQKAVKNSP+PRSYY+CTT  C VKKRVERSS DPS V+TT
Sbjct: 213 FLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDDPSIVMTT 272

Query: 205 YEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANFFS-GDPYKGVVDDHQDYS 258
           YEG+H H  P+T RG++  ++ P+         ++ FS   P   +   HQ Y+
Sbjct: 273 YEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQPRYLLTQHHQPYN 324

BLAST of MC02g0615 vs. TAIR 10
Match: AT2G47260.1 (WRKY DNA-binding protein 23 )

HSP 1 Score: 139.8 bits (351), Expect = 3.3e-33
Identity = 85/197 (43.15%), Postives = 114/197 (57.87%), Query Frame = 0

Query: 84  PSSSCSSDDRVAAGGAGGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKEKGEKGPRV 143
           P++  SS    A+  A   +  K+ D       + ++    KK+++  K  +K ++  RV
Sbjct: 103 PATPNSSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQKRQREARV 162

Query: 144 AFLTKTEIDNLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSSQDPSFVVT 203
           AF+TK+E+D+LEDGYRWRKYGQKAVKNSPFPRSYY+CTT +CNVKKRVERS +DPS VVT
Sbjct: 163 AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRDPSTVVT 222

Query: 204 TYEGKHNHYCPVTLRGNVAGMSLPLSSASPPWPPANFFSGDPYKGVVDDHQDYSQVIPDH 263
           TYEG+H H  P+T R    G     S A+      N   G P  G       + Q++  H
Sbjct: 223 TYEGQHTHISPLTSRPISTGGFFGSSGAASSL--GNGCFGFPIDGSTLISPQFQQLVQYH 282

Query: 264 YGLLQDLIPSSFNRKQE 281
           +   Q  + S F    E
Sbjct: 283 HQQQQQELMSCFGGVNE 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93WV42.6e-3846.02WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1[more]
Q9FL261.7e-3440.54WRKY transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=WRKY8 PE=1 SV=1[more]
Q8VWJ26.5e-3439.79WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1[more]
Q9FGZ41.6e-3247.13Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ... [more]
O229004.7e-3243.15WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022149678.18.10e-207100.00probable WRKY transcription factor 50 [Momordica charantia][more]
XP_038902581.11.34e-9958.23probable WRKY transcription factor 56 [Benincasa hispida][more]
XP_022943476.11.02e-9059.58probable WRKY transcription factor 28 isoform X1 [Cucurbita moschata][more]
XP_023512278.12.05e-9059.23probable WRKY transcription factor 48 [Cucurbita pepo subsp. pepo][more]
XP_011656990.17.66e-8961.29probable WRKY transcription factor 12 isoform X1 [Cucumis sativus] >KGN46828.1 h... [more]
Match NameE-valueIdentityDescription
A0A6J1D7F33.92e-207100.00probable WRKY transcription factor 50 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1FRU04.94e-9159.58probable WRKY transcription factor 28 isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A0A0KBC53.71e-8961.29WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139770 PE=4 S... [more]
A0A1S3C4A42.05e-8860.57probable WRKY transcription factor 12 OS=Cucumis melo OX=3656 GN=LOC103496315 PE... [more]
A0A6J1FT541.30e-7856.10probable WRKY transcription factor 48 isoform X2 OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT1G29860.11.8e-3946.02WRKY DNA-binding protein 71 [more]
AT5G46350.11.2e-3540.54WRKY DNA-binding protein 8 [more]
AT4G18170.14.6e-3539.79WRKY DNA-binding protein 28 [more]
AT5G49520.11.1e-3347.13WRKY DNA-binding protein 48 [more]
AT2G47260.13.3e-3343.15WRKY DNA-binding protein 23 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 154..213
e-value: 1.6E-38
score: 144.0
IPR003657WRKY domainPFAMPF03106WRKYcoord: 155..211
e-value: 5.7E-26
score: 90.6
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 149..214
score: 32.133339
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 141..207
e-value: 1.3E-33
score: 116.5
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 147..214
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..116
NoneNo IPR availablePANTHERPTHR31221:SF211WRKY TRANSCRIPTION FACTOR 71-RELATEDcoord: 24..232
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 24..232

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0615.1MC02g0615.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding