MC02g0596 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0596
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	heat stress transcription factor A-4c-like
Location	MC02: 4797523 .. 4798957 (-)
RNA-Seq Expression	MC02g0596
Synteny	MC02g0596

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGAAAATTCATCCTCCACAGCCAGCCTCTGGTCAACCCCTTTTTGAGCCTCTCTTTTCCTGACTTTTTCCACCACCGCGTCTATAAATCCCCCCTCCCCATTTCTTCATTTTTCAGCACAATCAAAATTCAAATCCTTCTGCTCATCAAAGATCTCAATAATTTTTTTTCTTTCTTTTTCTTCGAGTTCTGGTTTTGTTTCTGATCGCATCCAACTCTGATTTCTGTTCACTGAATTCTGAGAAACCCAGCGGCGACGACGGCGGCGCCGGAAATGGACAGTTCAGAGGTAAGCTCCGGCGCACCGCCGCCATTTTTGACTAAAACATACGAGATGGTGGACGACCCATCGACAAACGCCGTCGTATCGTGGAGTCAAAGCGGTTTCAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAATATATTTCAAGCACAACAATTTCTCCAGCTTCGTTCGCCAATTGAACACTTACGTGAGTTCGAATCAGACATCAAATTTCACAACCCCATCGTCGTTTTCCCCTGTTTTTTTTCCTAACATTAATCAATTCCTCTGTTTTTAGTTAAAATCCTGAGAGCAAAATTGTGGTATCTCTGTTTAGGGGTTCAGGAAGATCGATCGAGAGCAATGGGAATTCGCAAACGAGGGATTTGTAAGAGGACGAACCCATCTTCTAAGAACCATCCACCGACGGAAACCAATCTACAGCCACAGCCAGAACCAGAGCCATAGCCAGAACCAGGGCCAGAGCCATAGCCAGAGCTCCAATGGATTTCCATTTTCGGAACCAGAGAGGCAGGAGCTGGAGAACAAAATCAAGATCCTGAATCAAGAAAACACCCAACTTCAATCACAGCTGCAGAAGCACGAAAGAGAGAAAGAAGAAATCGAGCGCCAAATTCAGTCGCTCTGTAACCAATTATGGAGAATGGGGAATGAGCAGAAGCAGCTAATTACCATGCTGACGGCTCAATTGCAGAAGCAGCATCCGAGCAAAAAGAGGAGACTGTGGAAACTGAACGACTTATTGCTGGAAGACCCATCGGAGTGGGAGAAAGAGGAGAAGAATGGTTGGAAGAGCGTGATGATTCCGCCATTGGAGCTGATGGGGAAGCTTGAATCGTCGTTGGGGTTGTGCGAGGAGTTGCTGTGCAGCGTGGCGAAGGTGGTCGGAGAAGAATCGAAGGTGGTCGGAGAAGTCAAGGTTGTGGGGTCGAAGGAGGGGGATGTGAGAAATGGAGCGAATGATGTGTTTTGGGAACAGTTTCTGACGGAGATTCCAGGCTCTTCCAATGCAGGGGAAGTGATCTATTTGAATAGAAGGAATAATGTTGTAAATTAGAGAGAATTTAGATGTTGTAAATGATTCATTTCTGTAGCCTTTTTGCTCTTTAGGATTCTAATTGAACGTGGAAG

mRNA sequence

GTTGAAAATTCATCCTCCACAGCCAGCCTCTGGTCAACCCCTTTTTGAGCCTCTCTTTTCCTGACTTTTTCCACCACCGCGTCTATAAATCCCCCCTCCCCATTTCTTCATTTTTCAGCACAATCAAAATTCAAATCCTTCTGCTCATCAAAGATCTCAATAATTTTTTTTCTTTCTTTTTCTTCGAGTTCTGGTTTTGTTTCTGATCGCATCCAACTCTGATTTCTGTTCACTGAATTCTGAGAAACCCAGCGGCGACGACGGCGGCGCCGGAAATGGACAGTTCAGAGGTAAGCTCCGGCGCACCGCCGCCATTTTTGACTAAAACATACGAGATGGTGGACGACCCATCGACAAACGCCGTCGTATCGTGGAGTCAAAGCGGTTTCAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAATATATTTCAAGCACAACAATTTCTCCAGCTTCGTTCGCCAATTGAACACTTACGGGTTCAGGAAGATCGATCGAGAGCAATGGGAATTCGCAAACGAGGGATTTGTAAGAGGACGAACCCATCTTCTAAGAACCATCCACCGACGGAAACCAATCTACAGCCACAGCCAGAACCAGAGCCATAGCCAGAACCAGGGCCAGAGCCATAGCCAGAGCTCCAATGGATTTCCATTTTCGGAACCAGAGAGGCAGGAGCTGGAGAACAAAATCAAGATCCTGAATCAAGAAAACACCCAACTTCAATCACAGCTGCAGAAGCACGAAAGAGAGAAAGAAGAAATCGAGCGCCAAATTCAGTCGCTCTGTAACCAATTATGGAGAATGGGGAATGAGCAGAAGCAGCTAATTACCATGCTGACGGCTCAATTGCAGAAGCAGCATCCGAGCAAAAAGAGGAGACTGTGGAAACTGAACGACTTATTGCTGGAAGACCCATCGGAGTGGGAGAAAGAGGAGAAGAATGGTTGGAAGAGCGTGATGATTCCGCCATTGGAGCTGATGGGGAAGCTTGAATCGTCGTTGGGGTTGTGCGAGGAGTTGCTGTGCAGCGTGGCGAAGGTGGTCGGAGAAGAATCGAAGGTGGTCGGAGAAGTCAAGGTTGTGGGGTCGAAGGAGGGGGATGTGAGAAATGGAGCGAATGATGTGTTTTGGGAACAGTTTCTGACGGAGATTCCAGGCTCTTCCAATGCAGGGGAAGTGATCTATTTGAATAGAAGGAATAATGTTGTAAATTAGAGAGAATTTAGATGTTGTAAATGATTCATTTCTGTAGCCTTTTTGCTCTTTAGGATTCTAATTGAACGTGGAAG

Coding sequence (CDS)

ATGGACAGTTCAGAGGTAAGCTCCGGCGCACCGCCGCCATTTTTGACTAAAACATACGAGATGGTGGACGACCCATCGACAAACGCCGTCGTATCGTGGAGTCAAAGCGGTTTCAGCTTCGTGGTTTGGAACCCACCGGAATTCGCCAAAGAATTGCTCCCAATATATTTCAAGCACAACAATTTCTCCAGCTTCGTTCGCCAATTGAACACTTACGGGTTCAGGAAGATCGATCGAGAGCAATGGGAATTCGCAAACGAGGGATTTGTAAGAGGACGAACCCATCTTCTAAGAACCATCCACCGACGGAAACCAATCTACAGCCACAGCCAGAACCAGAGCCATAGCCAGAACCAGGGCCAGAGCCATAGCCAGAGCTCCAATGGATTTCCATTTTCGGAACCAGAGAGGCAGGAGCTGGAGAACAAAATCAAGATCCTGAATCAAGAAAACACCCAACTTCAATCACAGCTGCAGAAGCACGAAAGAGAGAAAGAAGAAATCGAGCGCCAAATTCAGTCGCTCTGTAACCAATTATGGAGAATGGGGAATGAGCAGAAGCAGCTAATTACCATGCTGACGGCTCAATTGCAGAAGCAGCATCCGAGCAAAAAGAGGAGACTGTGGAAACTGAACGACTTATTGCTGGAAGACCCATCGGAGTGGGAGAAAGAGGAGAAGAATGGTTGGAAGAGCGTGATGATTCCGCCATTGGAGCTGATGGGGAAGCTTGAATCGTCGTTGGGGTTGTGCGAGGAGTTGCTGTGCAGCGTGGCGAAGGTGGTCGGAGAAGAATCGAAGGTGGTCGGAGAAGTCAAGGTTGTGGGGTCGAAGGAGGGGGATGTGAGAAATGGAGCGAATGATGTGTTTTGGGAACAGTTTCTGACGGAGATTCCAGGCTCTTCCAATGCAGGGGAAGTGATCTATTTGAATAGAAGGAATAATGTTGTAAATTAG

Protein sequence

MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLELMGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIPGSSNAGEVIYLNRRNNVVN

Homology

[Close]

BLAST of MC02g0596 vs. ExPASy Swiss-Prot
Match: Q9FK72 (Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.0e-51
Identity = 134/334 (40.12%), Postives = 190/334 (56.89%), Query Frame = 0


Query: 1   MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60
           MD +   S + PPFLTKTYEMVDD S+++VV+WS++  SF+V NP EF+++LLP +FKH 
Sbjct: 1   MDENNGGSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKHK 60

Query: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120
           NFSSF+RQLNTYGFRK+D E+WEF N+ FVRGR +L++ IHRRKP++SHS     +QN  
Sbjct: 61  NFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN-- 120

Query: 121 QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180
                     P +E ER+ +E++I+ L  E   L ++LQ  E+E++E E Q+ +L ++L 
Sbjct: 121 ----------PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQ 180

Query: 181 RMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPP--- 240
            M   QK ++  ++  L K   S          L LE+     +  K  ++   +PP   
Sbjct: 181 HMEQHQKSIVAYVSQVLGKPGLS----------LNLEN----HERRKRRFQENSLPPSSS 240

Query: 241 -LELMGKLESSLGLCEELLCSVAKVVG-----------EESKVVGEVKVVGSK-----EG 300
            +E + KLESSL   E L+    +  G           E S  +G+ +   SK     E 
Sbjct: 241 HIEQVEKLESSLTFWENLVSESCEKSGLQSSSMDHDAAESSLSIGDTRPKSSKIDMNSEP 300

Query: 301 DV-------RNGANDVFWEQFLTEIPGSSNAGEV 308
            V       + G ND FWEQ LTE PGS+   EV
Sbjct: 301 PVTVTAPAPKTGVNDDFWEQCLTENPGSTEQQEV 308

[Close]

BLAST of MC02g0596 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.8e-49
Identity = 124/269 (46.10%), Postives = 167/269 (62.08%), Query Frame = 0


Query: 9   GAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFVRQ 68
           G PPPFL KTYEMV+D +TN VVSW   G SFVVWNP +F+++LLP YFKHNNFSSF+RQ
Sbjct: 16  GGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNNFSSFIRQ 75

Query: 69  LNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGQSHSQSSN 128
           LNTYGFRKID E+WEFANE F+RG THLL+ IHRRKP++SHS      QNQ        N
Sbjct: 76  LNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHS-----LQNQ-------IN 135

Query: 129 GFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNEQKQ 188
           G P +E ER+ELE +I  L  E + L + LQ+  +++  I  Q+Q++  +L  M   QK 
Sbjct: 136 G-PLAESERRELEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVAMEQRQKN 195

Query: 189 LITMLTAQLQKQ------------HPSKKRRLWKLNDLLLEDPSEWEKE-----EKNGWK 248
           ++  L   LQ++            H SKKRR+ K+ DL ++D +  E++     +  G  
Sbjct: 196 IVASLCEMLQRRGGAVSSSLLESDHFSKKRRVPKM-DLFVDDCAAGEEQKVFQFQGIGTD 255

Query: 249 SVMIPPL------ELMGKLESSLGLCEEL 255
           +  +PP+      E   ++E SL   E+L
Sbjct: 256 APAMPPVLPVTNGEAFDRVELSLVSLEKL 270

[Close]

BLAST of MC02g0596 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-48
Identity = 135/364 (37.09%), Postives = 193/364 (53.02%), Query Frame = 0


Query: 6   VSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSF 65
           VSS + PPFLTKTYEMVDD S++++VSWSQS  SF+VWNPPEF+++LLP +FKHNNFSSF
Sbjct: 8   VSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSSF 67

Query: 66  VRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGQSHSQ 125
           +RQLNTYGFRK D EQWEFAN+ FVRG+ HL++ IHRRKP++SHS     +Q        
Sbjct: 68  IRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLN------ 127

Query: 126 SSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNE 185
                P ++ ER  + N+I+ L +E   L  +L K + E+E  E Q++ L  +L  M   
Sbjct: 128 -----PLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKR 187

Query: 186 QKQLITMLTAQLQK------------QHPSKKRRLWKL----NDLLLEDPSEWEKEEKNG 245
           QK +++ ++  L+K            +   +KRR  ++    ++ +LE+        + G
Sbjct: 188 QKTMVSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENKTCVVVREEG 247

Query: 246 WKSVMIPPLE-LMGKLESSLGL--------CEELLCS----------------------V 305
             S      E  + +LESS+ +        CE +L S                      +
Sbjct: 248 STSPSSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSCI 307

Query: 306 AKVVGEESKVVGEVKVV-------GSKEGDV--------RNGANDVFWEQFLTEIPGSSN 308
              V    K     +++       GSKE +           GAND FW+QF +E PGS+ 
Sbjct: 308 QLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVAAPPPPPVAGANDGFWQQFFSENPGSTE 360

[Close]

BLAST of MC02g0596 vs. ExPASy Swiss-Prot
Match: Q94J16 (Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4B PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 9.0e-48
Identity = 109/211 (51.66%), Postives = 148/211 (70.14%), Query Frame = 0


Query: 9   GAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFVRQ 68
           G+ PPFL+KTYEMVDDPST+AVV W+ +G SFVV N PEF ++LLP YFKHNNFSSFVRQ
Sbjct: 8   GSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQPEFCRDLLPKYFKHNNFSSFVRQ 67

Query: 69  LNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGQSHSQSSN 128
           LNTYGFRK+D EQWEFANE F++G+ H L+ IHRRKPI+SHS   SHSQ  G        
Sbjct: 68  LNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRRKPIFSHS---SHSQGAG-------- 127

Query: 129 GFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNEQKQ 188
             P ++ ER++ E +I+ L  +N  L S+LQ +  +K  +E+++Q+L  +L+ + ++Q+ 
Sbjct: 128 --PLTDNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQALEEKLFVVEDQQRS 187

Query: 189 LIT----------MLTAQLQKQ-HPSKKRRL 209
           LI+           L++ +Q+Q H  KKRRL
Sbjct: 188 LISYVREIVKAPGFLSSFVQQQDHHRKKRRL 205

[Close]

BLAST of MC02g0596 vs. ExPASy Swiss-Prot
Match: P41153 (Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.2e-45
Identity = 108/236 (45.76%), Postives = 151/236 (63.98%), Query Frame = 0


Query: 7   SSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFV 66
           S+ APPPFL KTY+MVDDPST+ +VSWS +  SFVVW+PPEFAK+LLP YFKHNNFSSFV
Sbjct: 35  SANAPPPFLVKTYDMVDDPSTDKIVSWSPTNNSFVVWDPPEFAKDLLPKYFKHNNFSSFV 94

Query: 67  RQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQ----SHSQNQGQS 126
           RQLNTYGFRK+D ++WEFANEGF+RG+ HLL++I RRKP + H+Q Q     H+Q Q Q 
Sbjct: 95  RQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKSISRRKPAHGHAQQQQQPHGHAQQQMQP 154

Query: 127 HSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRM 186
              S++     E  +  LE +++ L ++   L  +L +  ++++  + Q+Q +  +L  M
Sbjct: 155 PGHSASVGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQGMVQRLQGM 214

Query: 187 GNEQKQLITMLT---------AQLQKQ---------HPSKKRRLWKLNDLLLEDPS 221
              Q+Q+++ L          AQ  +Q           SKKRR+    D+  +DPS
Sbjct: 215 ELRQQQMMSFLAKAVNSPGFLAQFVQQQNESNKRIAEGSKKRRI--KQDIESQDPS 268

[Close]

BLAST of MC02g0596 vs. NCBI nr
Match: XP_022149724.1 (heat stress transcription factor A-4c-like [Momordica charantia])

HSP 1 Score: 624 bits (1610), Expect = 2.18e-225
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0


Query: 1   MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60
           MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN
Sbjct: 1   MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60

Query: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120
           NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG
Sbjct: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120

Query: 121 QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180
           QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW
Sbjct: 121 QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180

Query: 181 RMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLEL 240
           RMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLEL
Sbjct: 181 RMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLEL 240

Query: 241 MGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIPG 300
           MGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIPG
Sbjct: 241 MGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIPG 300

Query: 301 SSNAGEVIYLNRRNNVVN 318
           SSNAGEVIYLNRRNNVVN
Sbjct: 301 SSNAGEVIYLNRRNNVVN 318

[Close]

BLAST of MC02g0596 vs. NCBI nr
Match: XP_022970770.1 (heat stress transcription factor A-4c-like [Cucurbita maxima])

HSP 1 Score: 406 bits (1043), Expect = 3.71e-139
Identity = 218/321 (67.91%), Postives = 265/321 (82.55%), Query Frame = 0


Query: 1   MDSSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+ S+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+ 
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
           G       +G P SE ERQELE KIK L+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 G-------SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLE 240
           WRMGN+QKQLI ++ A+LQK    K+R++ KL++ L E+  E E++EKNG K   +P LE
Sbjct: 181 WRMGNQQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDEKNGLK---VPALE 240

Query: 241 LMGKLESSLGLCEELLCSVAKVVGEE-SKVVGEVKVVGSKEGDVR--NGANDVFWEQFLT 300
           LMGKLE SLGLCE+LLC+VA+V+G E S+   E++  G KEG+ R  NG NDVFWEQFLT
Sbjct: 241 LMGKLEVSLGLCEDLLCNVAEVLGGEMSEKRKEMEGKGVKEGEKRGENGVNDVFWEQFLT 300

Query: 301 EIPGSSNAGEVIYLNRRNNVV 317
           E+PG SNAGEV YL+RR+NV+
Sbjct: 301 EVPGCSNAGEV-YLDRRSNVL 310

[Close]

BLAST of MC02g0596 vs. NCBI nr
Match: XP_023534238.1 (heat stress transcription factor A-4c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 403 bits (1036), Expect = 4.94e-138
Identity = 216/322 (67.08%), Postives = 265/322 (82.30%), Query Frame = 0


Query: 1   MDSSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+ S+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+ 
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
           G       +G P SE ERQELE KIK L+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 G-------SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKS-VMIPPL 240
           WRMGN+QKQLI ++ A+LQK    K+R++ KL++ L E+  E E++E+NG K+ + +P L
Sbjct: 181 WRMGNQQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLKVPAL 240

Query: 241 ELMGKLESSLGLCEELLCSVAKVVGEE-SKVVGEVKVVGSKEGDVR--NGANDVFWEQFL 300
           ELMGKLE SLGLCE+LLC+VA+V+G E S    E++  G KEG+ R  NG NDVFWEQFL
Sbjct: 241 ELMGKLEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFL 300

Query: 301 TEIPGSSNAGEVIYLNRRNNVV 317
           TE+PG SN GEV YL+RR+NV+
Sbjct: 301 TEVPGCSNGGEV-YLDRRSNVL 314

[Close]

BLAST of MC02g0596 vs. NCBI nr
Match: KAG7034870.1 (Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 403 bits (1036), Expect = 5.30e-138
Identity = 218/321 (67.91%), Postives = 266/321 (82.87%), Query Frame = 0


Query: 1   MDSSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+ S+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS  Q+Q
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQS--QSQ 120

Query: 121 GQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
            QSH    +G P SE ERQELE KIK L+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 SQSHG---SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKS-VMIPPL 240
           WRMGN+QKQLI ++ A+LQK    K+R++ KL++ L E+  E E++E+NG K+ + +P L
Sbjct: 181 WRMGNQQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLKVPAL 240

Query: 241 ELMGKLESSLGLCEELLCSVAKVVGEE-SKVVGEVKVVGSKEGDVR--NGANDVFWEQFL 300
           ELMGKLE SLGLCE+LLC+VA+V+G E S    E++  G KEG+ R  NG NDVFWEQFL
Sbjct: 241 ELMGKLEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFL 300

Query: 301 TEIPGSSNAGEVIYLNRRNNV 316
           TE+PG SN GEV YL+RR+NV
Sbjct: 301 TEVPGCSNGGEV-YLDRRSNV 315

[Close]

BLAST of MC02g0596 vs. NCBI nr
Match: KAG6604741.1 (Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 403 bits (1035), Expect = 7.01e-138
Identity = 216/321 (67.29%), Postives = 264/321 (82.24%), Query Frame = 0


Query: 1   MDSSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+ S+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+ 
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
           G       +G P SE ERQELE KIK L+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 G-------SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKS-VMIPPL 240
           WRMGN+QKQLI ++ A+LQK    K+R++ KL++ L E+  E E++E+NG K+ + +P L
Sbjct: 181 WRMGNQQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLKVPAL 240

Query: 241 ELMGKLESSLGLCEELLCSVAKVVGEE-SKVVGEVKVVGSKEGDVR--NGANDVFWEQFL 300
           ELMGKLE SLGLCE+LLC+VA+V+G E S    E++  G KEG+ R  NG NDVFWEQFL
Sbjct: 241 ELMGKLEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFL 300

Query: 301 TEIPGSSNAGEVIYLNRRNNV 316
           TE+PG SN GEV YL+RR+NV
Sbjct: 301 TEVPGCSNGGEV-YLDRRSNV 313

[Close]

BLAST of MC02g0596 vs. ExPASy TrEMBL
Match: A0A6J1D7W3 (heat stress transcription factor A-4c-like OS=Momordica charantia OX=3673 GN=LOC111018080 PE=3 SV=1)

HSP 1 Score: 624 bits (1610), Expect = 1.06e-225
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0


Query: 1   MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60
           MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN
Sbjct: 1   MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60

Query: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120
           NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG
Sbjct: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120

Query: 121 QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180
           QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW
Sbjct: 121 QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180

Query: 181 RMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLEL 240
           RMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLEL
Sbjct: 181 RMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLEL 240

Query: 241 MGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIPG 300
           MGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIPG
Sbjct: 241 MGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIPG 300

Query: 301 SSNAGEVIYLNRRNNVVN 318
           SSNAGEVIYLNRRNNVVN
Sbjct: 301 SSNAGEVIYLNRRNNVVN 318

[Close]

BLAST of MC02g0596 vs. ExPASy TrEMBL
Match: A0A6J1I3T7 (heat stress transcription factor A-4c-like OS=Cucurbita maxima OX=3661 GN=LOC111469660 PE=3 SV=1)

HSP 1 Score: 406 bits (1043), Expect = 1.79e-139
Identity = 218/321 (67.91%), Postives = 265/321 (82.55%), Query Frame = 0


Query: 1   MDSSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+ S+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS SQ+ 
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQSQSQSH 120

Query: 121 GQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
           G       +G P SE ERQELE KIK L+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 G-------SGAPLSELERQELELKIKTLHQEKTILQTQLQEHEHEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLE 240
           WRMGN+QKQLI ++ A+LQK    K+R++ KL++ L E+  E E++EKNG K   +P LE
Sbjct: 181 WRMGNQQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDEKNGLK---VPALE 240

Query: 241 LMGKLESSLGLCEELLCSVAKVVGEE-SKVVGEVKVVGSKEGDVR--NGANDVFWEQFLT 300
           LMGKLE SLGLCE+LLC+VA+V+G E S+   E++  G KEG+ R  NG NDVFWEQFLT
Sbjct: 241 LMGKLEVSLGLCEDLLCNVAEVLGGEMSEKRKEMEGKGVKEGEKRGENGVNDVFWEQFLT 300

Query: 301 EIPGSSNAGEVIYLNRRNNVV 317
           E+PG SNAGEV YL+RR+NV+
Sbjct: 301 EVPGCSNAGEV-YLDRRSNVL 310

[Close]

BLAST of MC02g0596 vs. ExPASy TrEMBL
Match: A0A6J1G8J3 (heat stress transcription factor A-4d-like OS=Cucurbita moschata OX=3662 GN=LOC111451807 PE=3 SV=1)

HSP 1 Score: 399 bits (1024), Expect = 1.49e-136
Identity = 216/322 (67.08%), Postives = 264/322 (81.99%), Query Frame = 0


Query: 1   MDSSEVS-SGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M+ S+ S  GAPPPFLTKTYEMVDDP TN+VVSWS+SG+SFVVWNPPEFAKELLP+YFKH
Sbjct: 1   MEGSDGSYGGAPPPFLTKTYEMVDDPMTNSVVSWSKSGYSFVVWNPPEFAKELLPVYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRK+DR+QWEFANEGF+RGRTHLL++IHRRKPI+SHSQ+QS     
Sbjct: 61  NNFSSFVRQLNTYGFRKVDRDQWEFANEGFIRGRTHLLKSIHRRKPIFSHSQSQS----- 120

Query: 121 GQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
            QSH    +G P SE ERQELE KIK L+QE T LQ+QLQ+HE EKE+I RQIQ++C Q+
Sbjct: 121 -QSHG---SGAPLSELERQELELKIKTLHQEKTILQTQLQEHENEKEQIGRQIQTMCQQI 180

Query: 181 WRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVM-IPPL 240
           WRMGN+QKQLI ++ A+LQK    K+R++ KL++ L E+  E E++E+NG K+ + +P L
Sbjct: 181 WRMGNQQKQLIAIMAAELQKDQSRKRRKIGKLSEFLGEEWLEVERDERNGLKNGLEVPAL 240

Query: 241 ELMGKLESSLGLCEELLCSVAKVVGEE-SKVVGEVKVVGSKEGDVR--NGANDVFWEQFL 300
           ELMGKLE SLGLCE+LLC+VA+V+G E S    E++  G KEG+ R  NG NDVFWEQFL
Sbjct: 241 ELMGKLEVSLGLCEDLLCNVAEVLGGEMSGKRKEMEGKGVKEGEKRGENGVNDVFWEQFL 300

Query: 301 TEIPGSSNAGEVIYLNRRNNVV 317
           TE+PG SN GEV YL+RR+NV+
Sbjct: 301 TEVPGCSNGGEV-YLDRRSNVL 312

[Close]

BLAST of MC02g0596 vs. ExPASy TrEMBL
Match: A0A0A0KBI1 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G169310 PE=3 SV=1)

HSP 1 Score: 395 bits (1014), Expect = 3.12e-135
Identity = 214/318 (67.30%), Postives = 254/318 (79.87%), Query Frame = 0


Query: 1   MDSSEVSS-GAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           MD SE SS GAPPPFLTKTYEMVDDP TN++VSW+QSGFSFVVWNPPEFA+ELLPIYFKH
Sbjct: 1   MDGSEGSSTGAPPPFLTKTYEMVDDPMTNSIVSWNQSGFSFVVWNPPEFAQELLPIYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRKIDREQWEFANEGF+RG+THLL++IHRRKPIYSHSQ+       
Sbjct: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFIRGKTHLLKSIHRRKPIYSHSQS------- 120

Query: 121 GQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
               SQ + G P SE ER ELE KIK L QE T LQSQLQKHE EKE+I  QIQ +C +L
Sbjct: 121 ----SQGNGGAPLSEQERHELEQKIKTLYQEKTNLQSQLQKHENEKEQIGHQIQRICEKL 180

Query: 181 WRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLE 240
           WRMGN+QKQLI +L A+L+K    KKR++ K+N+ L+E+ +E+EK+     K V +PPLE
Sbjct: 181 WRMGNQQKQLIGILGAELEKNEHRKKRKIGKVNEFLVEELTEFEKDNLKK-KKVNVPPLE 240

Query: 241 LMGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIP 300
           L+GKLE SLGLCE+LL +V +V+ E  ++  EVK    KEG++R+G NDVFWE FLTEIP
Sbjct: 241 LLGKLELSLGLCEDLLSNVGQVLKEGKEM--EVK----KEGEMRSGVNDVFWEHFLTEIP 299

Query: 301 GSSNAGEVIYLNRRNNVV 317
           GSSN  +V +L+RRNNVV
Sbjct: 301 GSSNVTQV-HLDRRNNVV 299

[Close]

BLAST of MC02g0596 vs. ExPASy TrEMBL
Match: A0A5D3BGW6 (Heat stress transcription factor A-4c-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold203G00240 PE=3 SV=1)

HSP 1 Score: 393 bits (1010), Expect = 1.27e-134
Identity = 214/318 (67.30%), Postives = 257/318 (80.82%), Query Frame = 0


Query: 1   MDSSE-VSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKH 60
           M SSE  SSGAPPPFLTKTYEMVDDP +N++VSWSQSGFSFVVWNPPEFA+ELLPIYFKH
Sbjct: 1   MVSSEGSSSGAPPPFLTKTYEMVDDPMSNSIVSWSQSGFSFVVWNPPEFAQELLPIYFKH 60

Query: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQ 120
           NNFSSFVRQLNTYGFRKIDREQWEFANEGF+RG+THLL++IHRRKP+YSHSQ+       
Sbjct: 61  NNFSSFVRQLNTYGFRKIDREQWEFANEGFIRGKTHLLKSIHRRKPMYSHSQS------- 120

Query: 121 GQSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQL 180
               SQ + G P SE ERQELE+KIK L QE T L+SQLQKHE EKE+I  QIQ +C +L
Sbjct: 121 ----SQGNGGAPLSEQERQELEHKIKTLYQEKTNLESQLQKHENEKEQIGHQIQRICEKL 180

Query: 181 WRMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPPLE 240
           WRMG++QKQLI +L A+L+K    KKR++ K+N+LL+E+ +E+E+++    K V + PLE
Sbjct: 181 WRMGHQQKQLIGILGAELEKHEQRKKRKMGKVNELLVEEWTEFERDDLKK-KKVNVSPLE 240

Query: 241 LMGKLESSLGLCEELLCSVAKVVGEESKVVGEVKVVGSKEGDVRNGANDVFWEQFLTEIP 300
           LMGKLE SL LCE+LLC+VA+V+ E  ++  EVK    KEG++R+G NDVFWE FLTEIP
Sbjct: 241 LMGKLELSLELCEDLLCNVAQVLKEGKEM--EVK----KEGEMRSGVNDVFWEHFLTEIP 299

Query: 301 GSSNAGEVIYLNRRNNVV 317
           GSS   EV YL+RRNNVV
Sbjct: 301 GSSKVTEV-YLDRRNNVV 299

[Close]

BLAST of MC02g0596 vs. TAIR 10
Match: AT5G45710.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.1e-52
Identity = 134/334 (40.12%), Postives = 190/334 (56.89%), Query Frame = 0


Query: 1   MDSSEVSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHN 60
           MD +   S + PPFLTKTYEMVDD S+++VV+WS++  SF+V NP EF+++LLP +FKH 
Sbjct: 1   MDENNGGSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKHK 60

Query: 61  NFSSFVRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQG 120
           NFSSF+RQLNTYGFRK+D E+WEF N+ FVRGR +L++ IHRRKP++SHS     +QN  
Sbjct: 61  NFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN-- 120

Query: 121 QSHSQSSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLW 180
                     P +E ER+ +E++I+ L  E   L ++LQ  E+E++E E Q+ +L ++L 
Sbjct: 121 ----------PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQ 180

Query: 181 RMGNEQKQLITMLTAQLQKQHPSKKRRLWKLNDLLLEDPSEWEKEEKNGWKSVMIPP--- 240
            M   QK ++  ++  L K   S          L LE+     +  K  ++   +PP   
Sbjct: 181 HMEQHQKSIVAYVSQVLGKPGLS----------LNLEN----HERRKRRFQENSLPPSSS 240

Query: 241 -LELMGKLESSLGLCEELLCSVAKVVG-----------EESKVVGEVKVVGSK-----EG 300
            +E + KLESSL   E L+    +  G           E S  +G+ +   SK     E 
Sbjct: 241 HIEQVEKLESSLTFWENLVSESCEKSGLQSSSMDHDAAESSLSIGDTRPKSSKIDMNSEP 300

Query: 301 DV-------RNGANDVFWEQFLTEIPGSSNAGEV 308
            V       + G ND FWEQ LTE PGS+   EV
Sbjct: 301 PVTVTAPAPKTGVNDDFWEQCLTENPGSTEQQEV 308

[Close]

BLAST of MC02g0596 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 194.5 bits (493), Expect = 1.3e-49
Identity = 135/364 (37.09%), Postives = 193/364 (53.02%), Query Frame = 0


Query: 6   VSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSF 65
           VSS + PPFLTKTYEMVDD S++++VSWSQS  SF+VWNPPEF+++LLP +FKHNNFSSF
Sbjct: 8   VSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFKHNNFSSF 67

Query: 66  VRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGQSHSQ 125
           +RQLNTYGFRK D EQWEFAN+ FVRG+ HL++ IHRRKP++SHS     +Q        
Sbjct: 68  IRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQLN------ 127

Query: 126 SSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNE 185
                P ++ ER  + N+I+ L +E   L  +L K + E+E  E Q++ L  +L  M   
Sbjct: 128 -----PLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKR 187

Query: 186 QKQLITMLTAQLQK------------QHPSKKRRLWKL----NDLLLEDPSEWEKEEKNG 245
           QK +++ ++  L+K            +   +KRR  ++    ++ +LE+        + G
Sbjct: 188 QKTMVSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENKTCVVVREEG 247

Query: 246 WKSVMIPPLE-LMGKLESSLGL--------CEELLCS----------------------V 305
             S      E  + +LESS+ +        CE +L S                      +
Sbjct: 248 STSPSSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSCI 307

Query: 306 AKVVGEESKVVGEVKVV-------GSKEGDV--------RNGANDVFWEQFLTEIPGSSN 308
              V    K     +++       GSKE +           GAND FW+QF +E PGS+ 
Sbjct: 308 QLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVAAPPPPPVAGANDGFWQQFFSENPGSTE 360

[Close]

BLAST of MC02g0596 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 182.2 bits (461), Expect = 6.6e-46
Identity = 96/196 (48.98%), Postives = 141/196 (71.94%), Query Frame = 0


Query: 6   VSSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSF 65
           +SS APPPFL+KTY+MVDD +T+++VSWS +  SF+VW PPEFA++LLP  FKHNNFSSF
Sbjct: 30  LSSNAPPPFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSF 89

Query: 66  VRQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGQSHSQ 125
           VRQLNTYGFRK+D ++WEFANEGF+RG+ HLL++I RRKP  +H Q Q H ++Q  S+ Q
Sbjct: 90  VRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKP--AHGQGQGHQRSQ-HSNGQ 149

Query: 126 SSNGFPFSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNE 185
           +S+     E  +  LE +++ L ++   L  +L +  ++++  + Q+Q++  +L  M N 
Sbjct: 150 NSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQTMVQRLQGMENR 209

Query: 186 QKQLITMLTAQLQKQH 202
           Q+QL++ L   +Q  H
Sbjct: 210 QQQLMSFLAKAVQSPH 222

[Close]

BLAST of MC02g0596 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 173.3 bits (438), Expect = 3.1e-43
Identity = 106/252 (42.06%), Postives = 153/252 (60.71%), Query Frame = 0


Query: 7   SSGAPPPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFV 66
           ++  PPPFL+KTY+MV+DP+T+A+VSWS +  SF+VW+PPEF+++LLP YFKHNNFSSFV
Sbjct: 46  ANSLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFV 105

Query: 67  RQLNTYGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGQSHSQS 126
           RQLNTYGFRK+D ++WEFANEGF+RG+ HLL+ I RRK +  H  + S+ Q+Q  S  Q 
Sbjct: 106 RQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQG 165

Query: 127 SNGFPFS--EPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGN 186
           S     S  E  +  LE +++ L ++   L  +L K  ++++  + ++Q L   L  M  
Sbjct: 166 SMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQ 225

Query: 187 EQKQLI----------TMLTAQLQKQHPS--------KKRRLWKLNDLLLEDPSEWEKEE 239
            Q+Q++          T L+  +QKQ  S        KKRRL + +    E  S     E
Sbjct: 226 RQQQIMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRLREDSTAATESNSHSHSLE 285

[Close]

BLAST of MC02g0596 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 167.2 bits (422), Expect = 2.2e-41
Identity = 94/187 (50.27%), Postives = 129/187 (68.98%), Query Frame = 0


Query: 12  PPFLTKTYEMVDDPSTNAVVSWSQSGFSFVVWNPPEFAKELLPIYFKHNNFSSFVRQLNT 71
           PPFL+KTY+MVDDP TN VVSWS    SFVVW+ PEF+K LLP YFKHNNFSSFVRQLNT
Sbjct: 26  PPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQLNT 85

Query: 72  YGFRKIDREQWEFANEGFVRGRTHLLRTIHRRKPIYSHSQNQSHSQNQGQSHSQSSNGFP 131
           YGFRK+D ++WEFANEGF+RGR  LL++I RRKP  SH Q     QNQ Q+  QSS+   
Sbjct: 86  YGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKP--SHVQ-----QNQQQTQVQSSSVGA 145

Query: 132 FSEPERQELENKIKILNQENTQLQSQLQKHEREKEEIERQIQSLCNQLWRMGNEQKQLIT 191
             E  +  +E +++ L ++   L  +L +  ++++  E Q+Q++  ++  M   Q+Q+++
Sbjct: 146 CVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMS 205

Query: 192 MLTAQLQ 199
            L   +Q
Sbjct: 206 FLAKAVQ 205

The following BLAST results are available for this feature:

BLAST of MC02g0596 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FK72	3.0e-51	40.12	Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C ...	[more]
Q93VB5	4.8e-49	46.10	Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
O49403	1.8e-48	37.09	Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ...	[more]
Q94J16	9.0e-48	51.66	Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P41153	3.2e-45	45.76	Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1	[more]

BLAST of MC02g0596 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149724.1	2.18e-225	100.00	heat stress transcription factor A-4c-like [Momordica charantia]	[more]
XP_022970770.1	3.71e-139	67.91	heat stress transcription factor A-4c-like [Cucurbita maxima]	[more]
XP_023534238.1	4.94e-138	67.08	heat stress transcription factor A-4c-like [Cucurbita pepo subsp. pepo]	[more]
KAG7034870.1	5.30e-138	67.91	Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. ar...	[more]
KAG6604741.1	7.01e-138	67.29	Heat stress transcription factor A-4b, partial [Cucurbita argyrosperma subsp. so...	[more]

BLAST of MC02g0596 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7W3	1.06e-225	100.00	heat stress transcription factor A-4c-like OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1I3T7	1.79e-139	67.91	heat stress transcription factor A-4c-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1G8J3	1.49e-136	67.08	heat stress transcription factor A-4d-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0KBI1	3.12e-135	67.30	HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G169310 ...	[more]
A0A5D3BGW6	1.27e-134	67.30	Heat stress transcription factor A-4c-like OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of MC02g0596 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45710.1	2.1e-52	40.12	winged-helix DNA-binding transcription factor family protein	[more]
AT4G18880.1	1.3e-49	37.09	heat shock transcription factor A4A	[more]
AT1G32330.1	6.6e-46	48.98	heat shock transcription factor A1D	[more]
AT4G17750.1	3.1e-43	42.06	heat shock factor 1	[more]
AT5G16820.1	2.2e-41	50.27	heat shock factor 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 137..185
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 108..133
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 108..135
None	No IPR available	PANTHER	PTHR10015:SF312	HEAT STRESS TRANSCRIPTION FACTOR A-4D	coord: 7..217
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PRINTS	PR00056	HSFDOMAIN	coord: 14..37 score: 50.48 coord: 52..64 score: 78.24 coord: 65..77 score: 84.62
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 10..103 e-value: 1.6E-61 score: 220.4
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 14..103 e-value: 1.4E-31 score: 108.9
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PROSITE	PS00434	HSF_DOMAIN	coord: 53..77
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 7..104 e-value: 1.1E-37 score: 130.6
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 7..217
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 10..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g0596.1	MC02g0596.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034605	cellular response to heat
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding