MC02g0587 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0587
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionLipase
LocationMC02: 4745547 .. 4749108 (+)
RNA-Seq ExpressionMC02g0587
SyntenyMC02g0587
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAACTATGTATGGAGGCATTTATGGCGCTAAATTGTGATCGGTGTAGATGAATGAATCAAAATGATAAGAGCAAGCTCAATCGAGTCATGACTCAATTTACAGGTTGGTATCATGCAATATCCAAATATACATTTCAATTATCATAGCGCAGCGATGCATTTCCTAATTGACGAACAAATTTTGTGATCTTCATATCGCTGTATCGACGATGGCGTTCACTTGAAATCGACCTGCAGCAGAAATTGGCATTGATGTAATCATCATCGAAGCATCCTAGGAAATTTCTTGTTCGAGTGTGGAATCTTGTAGGCGACGAACGCTAAGCTTTTCCTTGAGCGTAATGTTATTTTGAGTTTGACAGTGGGAAGACGTGTCGGCCCCACCAGGTACCCTCGATCAATGGCTTTTGATGAGCGGGAGCGGATGGCAATTCATGTGATTACTCAATTTCTGTTTTTATCTGGTCTTCTTCTGCGTCGATATAAAATTCCAAAACCCCTGTGTCGCACCATGAATCAAAATGAACTCGCCCAATCCCTTCACTTCGATCGTTCTTCTACTTCTGTTCTATGGCTCCGCATTTGGGATCAGAAGAGTTTCTGCATTGGAACCCGGGTCTGTCGATGGTATATGTAAATCGTTAGTGGAGCCACACGATTATGCTTGCGAAGAACATCTGGTAATTTGGCGAAAAGTTCTTCTTCTTCTTCTTCTTCAATACCCAATTGATTCACAGAATGATCTTCATTTGGGTTTCAGGTGATAACGAACGATGGGTTCATTCTCAGCATGCAGAGGATTCCATCAGGGCGGACGAGCTCAGCCAATGGGCCACCAGTTCTGCTACAGCACGGGCTTCTAATGGTTGGTAGTTCTGATTTTGGTGTCATTTTATGTTTATGGCCTGAAATTTTGATTCTAAGAGTGAGAATGATGGCCTTAGAGATTGCTCTTACAAACTATGTGGATGGGTGGTAGGATGCGGTTACATGGCTGATGCTTCCTCCAGAAAGTTCTTTGGCCTTCATTTTGGCAGATAAAGGGTTTGATGTGTGGCTTGCGAACACCCGTGGAACAAAATTTAGCCAAGGACACACATCACTCGGCCCTGATGATCCTGTAATTACTTAATTCACTAGTTCTCAGTTTCATTTATACGTTTCTAAAAGTAACATTGAGTTGTTATTTAGAGTTTCTGGGACTGGTCATGGGATGAACTGGTAGCTCTTGATCTACCTGCCACTCTCCAGTATGTTCATAACCATACTGGGCAGAAGATGCACTATGTTGGACATTCATTGGTTAGTATAAGTGTTACTGTTGCATATTTATTGACTTGTAAAAGTGTCTTATCTGTAACTGAGATGGAGAAGTTATTGGTTTGAAGGGAACACTAATTGCTCTTGCTGCATTTTCCAAACACCAATTGCTAAACATGTTGAGGTCAGCTGCTTTGATTAGCCCAATTGCCCATTTGGGTAAAATAACCTCGCCAATTGCGATAAATGCTGCAGATAACTTTCTTGGTGAGGTATAAATATCCATTCCTCCCCCGTATGTTACTGATCGAATTATAAGGTGCATTACCAATTCTTTTAATTTATCGTAAACAGGTTTTGTTCTGGTTGGGTGTCAAAGAGTTTGATCCCAGAGGGTAAATATGCAACATTTCTTTTTCTCCTCTGCTAAGGAAACTTTTAGATCAGTGAAATAACTAGTTCATTTAGTTCTTCTTTTGCACATTCTCTTGTTTGATGATTCATATGGGCAAGAGATTTTGACTTACTTCGAATGAACAAATAGGAAGGCTGCAGTCCAACTTCTTGTGGATGTTTGTGCAAAACCAGGTGTTGATTGCATCAACTTGTTGACCTCTTTCACAGGTTGCAATATTATGTTGTTCATTATTTTATTTCCTGACCTAGCTCTCGGAGAAAGACTTTTTGCTCGACTTGGATAATATAATTTTTTGTTACAATTATAGGCCAGAACTGCTGCCTCAATCCCTCTGTTACACAAATCTTCCTAACCCATGAGCCTCAGCCAACAGCAACAAGGAACATGATTCATCTGGCTCAGAGTAAGGACTAAATTTCTTATTAGAAAAAATCCAGACTGATTTATCCAGTCCAAATGCTATATGAATATGTAATGTGGCTTTGAGTATACTTGAATTTTTGTGTTGTTGTAAGTTTTGAACTCAAAAAAGTATACTAATAACTGCAAAATTTGTGCTGTCTCAATGTCGTTTTGGCAATTATGGGCAGTGATCAGAAGCGGAACCATAGCAATGTATGATTATGTTGACGTGATTGAAAACATGAAACACTACGGGCAACCAACTCCTCCAGAGTACAATATGACGAGCATTCCAAATGACTTCCCTCTCTTTCTTAGCTATGGAGGGGCTGATGCCCTGTCTGATGTCAATGATGTGCAGCTCTTGTTGAATAGCCTCAAAGACCATGATGGAGATAAGCTTGTAGTTCAGTTTAGAGAAGATTATGCACATGCTGATTTTGTCATGGGTGAAAACGCTAAGCAAGTTGTGTATGATCCCCTGATGGCTTTCTTGAAGCTTCAGTGAAGATATTGATGTTATGTGAATATCATGTCTATAACTAAACATCTAAGTGCATTTAGTTGTCGGTGTCCACGAACATGTAGAACAAGGCCTTGTAGGGAATATCACAATGGTCAACAAAGCTTACGGGTGGTTTCTACCTTGTTTGTAACTTCATGTGTAACTATATAAAGAATGATGCAAAAGGAAAAGAAACTCTTATACCAATATTTTGTGAATGGATTTTTGCTGTTAGTATTAGGTTCTATCCAGCCATACAATGCTCCAATATGTTCAGCCTGAATTTTCAGCTTTGAGATTTGATTGTATTGACAGTATGTTGGAAAAGGGAACTGGATGATAGTCAGGGAATAAAGAATGGCGTCTTCGAACAATGAACCAACGACGCTTCATTATCGTTCGGCAAGAGAATGAAGCAAACCGAATGGATTTAGCAAAATATTGGCACACAAACAAGGTTTCTTCTATATCATGGGAAATACAAGAGTTAAAAACAGCAGCATCATTCATTCAGAAAGGAACATGACCTCTTTTTCTTCCTCAAAGATAGATGTTTGGCCTGATACCGAAGTGATTCTGTGCTGCAAGGTGAGCTGCAACGAAAGGATCACGATCGACACTGTAGCTAACTGCACCGTAACACTTTTTCCTTTTCGATGGCAGGAAGAGCTCTTCACAGAAAATTGCGCACCGAGGTGAGGCCAAAGTCCCTTGCTCGGGTTTCTTACACTGTAAGGACACCAACATTTTCTTCAGTTTCCTTAAACACTTCACAAGCTTCTTCATTCTCACCACACTGGTTGGCTGGCTTGTTCGGTTGTATTTGTAACTCAATTTATTGTGTGCCAATCACATAGAATTTGTGGTGGTTTTGCTTCCAAGTAGTTACTTCATGCATGAATGAACAAGTACCTAATAGACTTGTCCTTTTCTTGCTTTTAGAAACTCACAGCTTGTGTGGCATGGAACTTGGAG

mRNA sequence

AAAAACTATGTATGGAGGCATTTATGGCGCTAAATTGTGATCGGTGTAGATGAATGAATCAAAATGATAAGAGCAAGCTCAATCGAGTCATGACTCAATTTACAGAAATTGGCATTGATGTAATCATCATCGAAGCATCCTAGGAAATTTCTTGTTCGAGTGTGGAATCTTGTAGGCGACGAACGCTAAGCTTTTCCTTGAGCGTAATGTTATTTTGAGTTTGACAGTGGGAAGACGTGTCGGCCCCACCAGGTACCCTCGATCAATGGCTTTTGATGAGCGGGAGCGGATGGCAATTCATGTGATTACTCAATTTCTGTTTTTATCTGGTCTTCTTCTGCGTCGATATAAAATTCCAAAACCCCTGTGTCGCACCATGAATCAAAATGAACTCGCCCAATCCCTTCACTTCGATCGTTCTTCTACTTCTGTTCTATGGCTCCGCATTTGGGATCAGAAGAGTTTCTGCATTGGAACCCGGGTCTGTCGATGGTATATGTAAATCGTTAGTGGAGCCACACGATTATGCTTGCGAAGAACATCTGGTGATAACGAACGATGGGTTCATTCTCAGCATGCAGAGGATTCCATCAGGGCGGACGAGCTCAGCCAATGGGCCACCAGTTCTGCTACAGCACGGGCTTCTAATGGATGCGGTTACATGGCTGATGCTTCCTCCAGAAAGTTCTTTGGCCTTCATTTTGGCAGATAAAGGGTTTGATGTGTGGCTTGCGAACACCCGTGGAACAAAATTTAGCCAAGGACACACATCACTCGGCCCTGATGATCCTAGTTTCTGGGACTGGTCATGGGATGAACTGGTAGCTCTTGATCTACCTGCCACTCTCCAGTATGTTCATAACCATACTGGGCAGAAGATGCACTATGTTGGACATTCATTGGGAACACTAATTGCTCTTGCTGCATTTTCCAAACACCAATTGCTAAACATGTTGAGGTCAGCTGCTTTGATTAGCCCAATTGCCCATTTGGGTAAAATAACCTCGCCAATTGCGATAAATGCTGCAGATAACTTTCTTGGTGAGGTTTTGTTCTGGTTGGGTGTCAAAGAGTTTGATCCCAGAGGGAAGGCTGCAGTCCAACTTCTTGTGGATGTTTGTGCAAAACCAGGTGTTGATTGCATCAACTTGTTGACCTCTTTCACAGGCCAGAACTGCTGCCTCAATCCCTCTGTTACACAAATCTTCCTAACCCATGAGCCTCAGCCAACAGCAACAAGGAACATGATTCATCTGGCTCAGATGATCAGAAGCGGAACCATAGCAATGTATGATTATGTTGACGTGATTGAAAACATGAAACACTACGGGCAACCAACTCCTCCAGAGTACAATATGACGAGCATTCCAAATGACTTCCCTCTCTTTCTTAGCTATGGAGGGGCTGATGCCCTGTCTGATGTCAATGATGTGCAGCTCTTGTTGAATAGCCTCAAAGACCATGATGGAGATAAGCTTGTAGTTCAGTTTAGAGAAGATTATGCACATGCTGATTTTGTCATGGGTGAAAACGCTAAGCAAGTTGTGTATGATCCCCTGATGGCTTTCTTGAAGCTTCAGTGAAGATATTGATGTTATGTGAATATCATGTCTATAACTAAACATCTAAGTGCATTTAGTTGTCGGTGTCCACGAACATGTAGAACAAGGCCTTGTAGGGAATATCACAATGGTCAACAAAGCTTACGGGTGGTTTCTACCTTGTTTGTAACTTCATGTGTAACTATATAAAGAATGATGCAAAAGGAAAAGAAACTCTTATACCAATATTTTGTGAATGGATTTTTGCTGTTAGTATTAGGTTCTATCCAGCCATACAATGCTCCAATATGTTCAGCCTGAATTTTCAGCTTTGAGATTTGATTGTATTGACAGTATGTTGGAAAAGGGAACTGGATGATAGTCAGGGAATAAAGAATGGCGTCTTCGAACAATGAACCAACGACGCTTCATTATCGTTCGGCAAGAGAATGAAGCAAACCGAATGGATTTAGCAAAATATTGGCACACAAACAAGGTTTCTTCTATATCATGGGAAATACAAGAGTTAAAAACAGCAGCATCATTCATTCAGAAAGGAACATGACCTCTTTTTCTTCCTCAAAGATAGATGTTTGGCCTGATACCGAAGTGATTCTGTGCTGCAAGGTGAGCTGCAACGAAAGGATCACGATCGACACTGTAGCTAACTGCACCGTAACACTTTTTCCTTTTCGATGGCAGGAAGAGCTCTTCACAGAAAATTGCGCACCGAGGTGAGGCCAAAGTCCCTTGCTCGGGTTTCTTACACTGTAAGGACACCAACATTTTCTTCAGTTTCCTTAAACACTTCACAAGCTTCTTCATTCTCACCACACTGGTTGGCTGGCTTGTTCGGTTGTATTTGTAACTCAATTTATTGTGTGCCAATCACATAGAATTTGTGGTGGTTTTGCTTCCAAGTAGTTACTTCATGCATGAATGAACAAGTACCTAATAGACTTGTCCTTTTCTTGCTTTTAGAAACTCACAGCTTGTGTGGCATGGAACTTGGAG

Coding sequence (CDS)

ATGAACTCGCCCAATCCCTTCACTTCGATCGTTCTTCTACTTCTGTTCTATGGCTCCGCATTTGGGATCAGAAGAGTTTCTGCATTGGAACCCGGGTCTGTCGATGGTATATGTAAATCGTTAGTGGAGCCACACGATTATGCTTGCGAAGAACATCTGGTGATAACGAACGATGGGTTCATTCTCAGCATGCAGAGGATTCCATCAGGGCGGACGAGCTCAGCCAATGGGCCACCAGTTCTGCTACAGCACGGGCTTCTAATGGATGCGGTTACATGGCTGATGCTTCCTCCAGAAAGTTCTTTGGCCTTCATTTTGGCAGATAAAGGGTTTGATGTGTGGCTTGCGAACACCCGTGGAACAAAATTTAGCCAAGGACACACATCACTCGGCCCTGATGATCCTAGTTTCTGGGACTGGTCATGGGATGAACTGGTAGCTCTTGATCTACCTGCCACTCTCCAGTATGTTCATAACCATACTGGGCAGAAGATGCACTATGTTGGACATTCATTGGGAACACTAATTGCTCTTGCTGCATTTTCCAAACACCAATTGCTAAACATGTTGAGGTCAGCTGCTTTGATTAGCCCAATTGCCCATTTGGGTAAAATAACCTCGCCAATTGCGATAAATGCTGCAGATAACTTTCTTGGTGAGGTTTTGTTCTGGTTGGGTGTCAAAGAGTTTGATCCCAGAGGGAAGGCTGCAGTCCAACTTCTTGTGGATGTTTGTGCAAAACCAGGTGTTGATTGCATCAACTTGTTGACCTCTTTCACAGGCCAGAACTGCTGCCTCAATCCCTCTGTTACACAAATCTTCCTAACCCATGAGCCTCAGCCAACAGCAACAAGGAACATGATTCATCTGGCTCAGATGATCAGAAGCGGAACCATAGCAATGTATGATTATGTTGACGTGATTGAAAACATGAAACACTACGGGCAACCAACTCCTCCAGAGTACAATATGACGAGCATTCCAAATGACTTCCCTCTCTTTCTTAGCTATGGAGGGGCTGATGCCCTGTCTGATGTCAATGATGTGCAGCTCTTGTTGAATAGCCTCAAAGACCATGATGGAGATAAGCTTGTAGTTCAGTTTAGAGAAGATTATGCACATGCTGATTTTGTCATGGGTGAAAACGCTAAGCAAGTTGTGTATGATCCCCTGATGGCTTTCTTGAAGCTTCAGTGA

Protein sequence

MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAAFSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQLLVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ
Homology
BLAST of MC02g0587 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 6.5e-112
Identity = 189/368 (51.36%), Postives = 255/368 (69.29%), Query Frame = 0

Query: 36  GICKSLVEPHDYACEEHLVITNDGFILSMQRIPSGRTSSANG-----PPVLLQHGLLMDA 95
           GIC S V    Y CEEH V+T DG+IL+MQRIP GR  +  G      PVL+QHG+L+D 
Sbjct: 50  GICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDG 109

Query: 96  VTWLMLPPESSLAFILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDL 155
           ++WL+ P + +L  ILAD+GFDVW+ NTRGT+FS+ H  L P   +FW+W+WDELV+ DL
Sbjct: 110 MSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDL 169

Query: 156 PATLQYVHNHTGQKMHYVGHSLGTLIALAAFSKHQLLNMLRSAALISPIAHLGKITSPIA 215
           PA   ++H  TGQK+HY+GHSLGTLI  A+FS+  L++ +RSAA++SP+A+L  +T+ I 
Sbjct: 170 PAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIG 229

Query: 216 INAADNFLGEVLFWLGVKEFDPRGKAAVQLLVDVCAKPGVDCINLLTSFTGQNCCLNPSV 275
             AA  FL E    LG  EF+P+       +  +C K G+DC +L++  TG+NCCLN S 
Sbjct: 230 DIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAST 289

Query: 276 TQIFLTHEPQPTATRNMIHLAQMIRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIPND 335
             +FL +EPQ T+T+NMIHLAQ +R   +  Y+Y     N+KHYGQ  PP YN+++IP++
Sbjct: 290 IDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHE 349

Query: 336 FPLFLSYGGADALSDVNDVQLLLNSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDP 395
            PLF SYGG D+L+DV DV+ LL+  K HD DK+ VQF +DYAHADF+MG  AK VVY+ 
Sbjct: 350 LPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQ 409

Query: 396 LMAFLKLQ 399
           +  F K Q
Sbjct: 410 VATFFKRQ 417

BLAST of MC02g0587 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 4.4e-84
Identity = 149/368 (40.49%), Postives = 225/368 (61.14%), Query Frame = 0

Query: 29  LEPGSVDGICKSLVEPHDYACEEHLVITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLM 88
           L    V+ +C  L+ P +Y+C EH + T DG+IL++QR+ S      +GPPVLLQHGL M
Sbjct: 24  LHGSPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQSGPPVLLQHGLFM 83

Query: 89  DAVTWLMLPPESSLAFILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVAL 148
               W +  P+ SL FILAD GFDVW+ N RGT++S GH +L   D  FWDWSW +L   
Sbjct: 84  AGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMY 143

Query: 149 DLPATLQYVHNHTGQKMHYVGHSLGTLIALAAFSKHQLLNMLRSAALISPIAHLGKITSP 208
           DL   +QY+++ +  K+  VGHS GT+++ AA ++  +  M+ +AAL+ PI++L  +T+P
Sbjct: 144 DLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAP 203

Query: 209 IAINAADNFLGEVLFWLGVKEFDPRGKAAVQLLVDVCAKPGVDCINLLTSFTGQNCCLNP 268
           +        L +++  LG+ + + R    V+L+  +C +  +DC + LTS TG NCC N 
Sbjct: 204 LVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLC-EGHMDCTDFLTSITGTNCCFNA 263

Query: 269 SVTQIFLTHEPQPTATRNMIHLAQMIRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIP 328
           S  + +L +EP P++ +N+ HL QMIR GT A YDY    +N++ YG   PPE+ ++ IP
Sbjct: 264 SKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDY-GYFKNLRTYGLSKPPEFILSHIP 323

Query: 329 NDFPLFLSYGGADALSDVNDVQLLLNSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVY 388
              P+++ YGG D L+DV DV+  L  L         + + EDY H DFV+G +AK+ VY
Sbjct: 324 ASLPMWMGYGGTDGLADVTDVEHTLAELPSSP----ELLYLEDYGHIDFVLGSSAKEDVY 383

Query: 389 DPLMAFLK 397
             ++ F +
Sbjct: 384 KHMIQFFR 385

BLAST of MC02g0587 vs. ExPASy Swiss-Prot
Match: P04634 (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 8.3e-59
Identity = 141/397 (35.52%), Postives = 220/397 (55.42%), Query Frame = 0

Query: 10  IVLLLLFYGSAFGIRRVSALEPGSVDG--ICKSLVEPHDYACEEHLVITNDGFILSMQRI 69
           I  ++  +G A G+     L PG+ +       ++    Y C+E+ V+T DG+IL + RI
Sbjct: 6   ITSVISTFGGAHGL--FGKLGPGNPEANMNISQMITYWGYPCQEYEVVTEDGYILGVYRI 65

Query: 70  PSGRTSSAN---GPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRGTKFS 129
           P G+ +S N    P V LQHGL+  A  W+   P +SLAF+LAD G+DVWL N+RG  +S
Sbjct: 66  PHGKNNSENIGKRPVVYLQHGLIASATNWIANLPNNSLAFMLADAGYDVWLGNSRGNTWS 125

Query: 130 QGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQ-KMHYVGHSLGTLIALAAFSK 189
           + +    PD   FW +S+DE+   DLPAT+ ++   TGQ K+HYVGHS GT I   AFS 
Sbjct: 126 RKNVYYSPDSVEFWAFSFDEMAKYDLPATINFIVQKTGQEKIHYVGHSQGTTIGFIAFST 185

Query: 190 H-QLLNMLRSAALISPIAHLGKITSPI-AINAADNFLGEVLFWLGVKEFDPRGKAAVQLL 249
           +  L   +++   ++P+A +    SP+  I+    FL +++F  G K F P       L 
Sbjct: 186 NPTLAKKIKTFYALAPVATVKYTQSPLKKISFIPTFLFKLMF--GKKMFLPHTYFDDFLG 245

Query: 250 VDVCAKPGVD--CINLLTSFTG-QNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGT 309
            +VC++  +D  C N L  F G     LN S   ++L H P  T+ ++ +H AQ++RSG 
Sbjct: 246 TEVCSREVLDLLCSNTLFIFCGFDKKNLNVSRFDVYLGHNPAGTSVQDFLHWAQLVRSGK 305

Query: 310 IAMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKD 369
              +++    +NM HY Q TPPEY+++++    P+ +  GG D L+D  DV +LL  L +
Sbjct: 306 FQAFNWGSPSQNMLHYNQKTPPEYDVSAM--TVPVAVWNGGNDILADPQDVAMLLPKLSN 365

Query: 370 HDGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFL 396
               K ++     Y H DF+   +A Q VY+ +++ +
Sbjct: 366 LLFHKEILA----YNHLDFIWAMDAPQEVYNEMISMM 392

BLAST of MC02g0587 vs. ExPASy Swiss-Prot
Match: P80035 (Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV=2)

HSP 1 Score: 227.6 bits (579), Expect = 2.4e-58
Identity = 135/361 (37.40%), Postives = 205/361 (56.79%), Query Frame = 0

Query: 47  YACEEHLVITNDGFILSMQRIPSGRTSSAN---GPPVLLQHGLLMDAVTWLMLPPESSLA 106
           Y  EE+ V+T DG+IL + RIP GR +S N    P   LQHGLL  A  W+   P +SLA
Sbjct: 44  YPAEEYEVVTEDGYILGIDRIPYGRKNSENIGRRPVAFLQHGLLASATNWISNLPNNSLA 103

Query: 107 FILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQ 166
           FILAD G+DVWL N+RG  +++ +    PD   FW +S+DE+   DLPAT+ ++   TGQ
Sbjct: 104 FILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFILKKTGQ 163

Query: 167 -KMHYVGHSLGTLIALAAFSKH-QLLNMLRSAALISPIAHLGKITSPI--AINAADNFLG 226
            K+HYVGHS GT I   AFS + +L   +++   ++P+A + K T  +   +    +FL 
Sbjct: 164 DKLHYVGHSQGTTIGFIAFSTNPKLAKRIKTFYALAPVATV-KYTETLLNKLMLVPSFLF 223

Query: 227 EVLFWLGVKEFDPRGKAAVQLLVDVCAKPGVD--CINLLTSFTG-QNCCLNPSVTQIFLT 286
           +++F  G K F P       L  +VC++  VD  C N L    G     LN S   ++L+
Sbjct: 224 KLIF--GNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFDTMNLNMSRLDVYLS 283

Query: 287 HEPQPTATRNMIHLAQMIRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLS 346
           H P  T+ +N++H +Q ++SG    +D+   ++NM HY Q  PP YN+T +    P+ + 
Sbjct: 284 HNPAGTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYYNLTDM--HVPIAVW 343

Query: 347 YGGADALSDVNDVQLLLNSLKDHDGDKLVVQFRE--DYAHADFVMGENAKQVVYDPLMAF 396
            GG D L+D +DV LLL+ L +      ++  R+   Y H DF+   +A Q VY+ +++ 
Sbjct: 344 NGGNDLLADPHDVDLLLSKLPN------LIYHRKIPPYNHLDFIWAMDAPQAVYNEIVSM 393

BLAST of MC02g0587 vs. ExPASy Swiss-Prot
Match: Q9CPP7 (Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.0e-57
Identity = 135/358 (37.71%), Postives = 201/358 (56.15%), Query Frame = 0

Query: 47  YACEEHLVITNDGFILSMQRIPSGRTSSAN---GPPVLLQHGLLMDAVTWLMLPPESSLA 106
           Y  EE+ V+T DG+IL + RIP G+ +S N    P   LQHGL+  A  W+   P +SLA
Sbjct: 43  YPSEEYEVVTEDGYILGVYRIPYGKKNSENIGKRPVAYLQHGLIASATNWITNLPNNSLA 102

Query: 107 FILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQ 166
           FILAD G+DVWL N+RG  +S+ +    PD   FW +S+DE+   DLPAT+ ++   TGQ
Sbjct: 103 FILADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIDFIVQKTGQ 162

Query: 167 -KMHYVGHSLGTLIALAAFSKH-QLLNMLRSAALISPIAHLGKITSPI-AINAADNFLGE 226
            K+HYVGHS GT I   AFS +  L   ++    ++P+A +    SP   I+    FL +
Sbjct: 163 EKIHYVGHSQGTTIGFIAFSTNPALAKKIKRFYALAPVATVKYTESPFKKISLIPKFLLK 222

Query: 227 VLFWLGVKEFDPRGKAAVQLLVDVCAKPGVD--CINLLTSFTG-QNCCLNPSVTQIFLTH 286
           V+F  G K F P       L  +VC++  +D  C N L  F G     LN S   ++L H
Sbjct: 223 VIF--GNKMFMPHNYLDQFLGTEVCSRELLDLLCSNALFIFCGFDKKNLNVSRFDVYLGH 282

Query: 287 EPQPTATRNMIHLAQMIRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSY 346
            P  T+T+++ H AQ+ +SG +  Y++   ++NM HY Q TPP Y+++++    P+ +  
Sbjct: 283 NPAGTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYDVSAM--TVPIAVWN 342

Query: 347 GGADALSDVNDVQLLLNSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFL 396
           GG D L+D  DV +LL  L +    K ++     Y H DF+   +A Q VY+ ++  +
Sbjct: 343 GGHDILADPQDVAMLLPKLPNLLYHKEIL----PYNHLDFIWAMDAPQEVYNEIVTMM 392

BLAST of MC02g0587 vs. NCBI nr
Match: XP_038901645.1 (triacylglycerol lipase 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 763 bits (1971), Expect = 1.05e-277
Identity = 367/398 (92.21%), Postives = 385/398 (96.73%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           M SPNPF  IVLLLLF G A GI+RVSALEPGSVDGICKSLVEPHDYACEEHLVIT+DGF
Sbjct: 1   MYSPNPFKFIVLLLLFCGLAAGIKRVSALEPGSVDGICKSLVEPHDYACEEHLVITSDGF 60

Query: 61  ILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRG 120
           ILSMQRIPSGRT+SANGPPVLLQHGLLMDAVTWLMLPPESSLAF+LADKGFDVWLANTRG
Sbjct: 61  ILSMQRIPSGRTNSANGPPVLLQHGLLMDAVTWLMLPPESSLAFLLADKGFDVWLANTRG 120

Query: 121 TKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAA 180
           TKFSQGHTSLGPDDP FWDWSWDELVA DLPATLQYVH+HTGQKMHYVGHSLGTLIALAA
Sbjct: 121 TKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLIALAA 180

Query: 181 FSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQL 240
           FSKHQLL+MLRSAALISPIAHLGK+TSPIA NAADNFLGEVLFWLGVKEFDPRGKA +QL
Sbjct: 181 FSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGIQL 240

Query: 241 LVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTIA 300
           LV+VCAKPGVDC+NLLTSFTGQNCCLNPSV+QIFLTHEPQPTAT+NMIHL+QMIRSGTI 
Sbjct: 241 LVEVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTIT 300

Query: 301 MYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHD 360
           MYDYVD+I+NMKHYGQPTPPEYNM SIPNDFPLFLSYGGADALSDVNDVQLLL++LKDHD
Sbjct: 301 MYDYVDIIQNMKHYGQPTPPEYNMASIPNDFPLFLSYGGADALSDVNDVQLLLDNLKDHD 360

Query: 361 GDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           GDKLVVQFREDYAHADFVMGENAKQVVYDPL+AF KLQ
Sbjct: 361 GDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFKLQ 398

BLAST of MC02g0587 vs. NCBI nr
Match: XP_008456397.1 (PREDICTED: triacylglycerol lipase 2-like [Cucumis melo] >TYK14548.1 triacylglycerol lipase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 752 bits (1942), Expect = 2.76e-273
Identity = 361/398 (90.70%), Postives = 382/398 (95.98%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           M SPN F SIVLLLLFYG A GI+RVSALEPGSV+GICKSLVEPHD+ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVLLLLFYGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRG 120
           ILSMQRIPSGRT+SANGPPVLLQHGLLMDA TWLMLPPESSLAF+LADKGFDVWLANTRG
Sbjct: 61  ILSMQRIPSGRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTRG 120

Query: 121 TKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAA 180
           TKFSQGH+SLGPDDP FWDWSWDELVA DLPATLQYVH+HTGQKMHYVGHSLGTLIALAA
Sbjct: 121 TKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLIALAA 180

Query: 181 FSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQL 240
           FSKHQLL+MLRSAALISPIAHLGK+TSPIA NAADNFLGEVLFWLGVKEFDPRGKA VQL
Sbjct: 181 FSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGVQL 240

Query: 241 LVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTIA 300
           LVDVCAKPGVDC+NLLTSFTGQNCCLNPSV+QIFLTHEPQPTAT+NMIHL+QMIRSGTI+
Sbjct: 241 LVDVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTIS 300

Query: 301 MYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHD 360
           MYDYVDVI+N+KHYGQPTPPEYNM SIP DFPLFL+YGGADALSDVNDVQLLL++LKDHD
Sbjct: 301 MYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDHD 360

Query: 361 GDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           GDKLVVQFREDYAHADFVMG+NAKQ VYDPL+AF  LQ
Sbjct: 361 GDKLVVQFREDYAHADFVMGKNAKQAVYDPLIAFFNLQ 398

BLAST of MC02g0587 vs. NCBI nr
Match: KGN47007.1 (hypothetical protein Csa_020982 [Cucumis sativus])

HSP 1 Score: 739 bits (1909), Expect = 2.95e-268
Identity = 355/398 (89.20%), Postives = 377/398 (94.72%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           M SPN F SIV  LLF G A GI+RVSALEPGSV+GICKSLVEPHD+ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVFSLLFCGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRG 120
           ILSMQRIPS RT+SANGPPVLLQHGLLMDA TWLMLPPESSLAF+LADKGFDVWLANTRG
Sbjct: 61  ILSMQRIPSRRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTRG 120

Query: 121 TKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAA 180
           TKFSQGH+SLGPDDP FWDWSWDELVA DLPATLQYVH+HTGQKMHYVGHSLGTL ALAA
Sbjct: 121 TKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLTALAA 180

Query: 181 FSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQL 240
           FSKHQLL+MLRSAALISPIAHLGK+TSPIA NAADNFLGEVLFWLGVKEFDPRGKA +QL
Sbjct: 181 FSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGIQL 240

Query: 241 LVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTIA 300
           LV+VCAKPGVDC+NLLTSFTGQNCCLNPSV+QIFLTHEPQPTAT+NMIHL+QMIRSGTI+
Sbjct: 241 LVEVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTIS 300

Query: 301 MYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHD 360
           MYDYVDVI+N+KHYGQPTPPEYNM SIP DFPLFL+YGGADALSDVNDVQLLL++LKDHD
Sbjct: 301 MYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDHD 360

Query: 361 GDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           GDKLVVQFREDYAHADFVMGENAKQ VYDPL+AF  LQ
Sbjct: 361 GDKLVVQFREDYAHADFVMGENAKQAVYDPLIAFFNLQ 398

BLAST of MC02g0587 vs. NCBI nr
Match: KAG6604747.1 (Triacylglycerol lipase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034875.1 Triacylglycerol lipase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 726 bits (1874), Expect = 5.68e-263
Identity = 358/399 (89.72%), Postives = 372/399 (93.23%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           MNSP+PF  IVLLLL  G       VSALEPGSVDGICKSLVEPH+YACEEHLVITNDGF
Sbjct: 1   MNSPDPFNPIVLLLLLCGFII----VSALEPGSVDGICKSLVEPHNYACEEHLVITNDGF 60

Query: 61  ILSMQRIPSG-RTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTR 120
           ILSMQRIPSG RTSSANGPPVLLQHGLLMDA TWL+LPPESSLAF+LADKGFDVWLANTR
Sbjct: 61  ILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTR 120

Query: 121 GTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALA 180
           GTKFSQGHTSLGPDDP FWDWSWDELVA DLPATLQYVHN TGQ MHYVGHSLGTLIALA
Sbjct: 121 GTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALA 180

Query: 181 AFSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQ 240
           AFSKHQLL+ML SAALISPIAHLG +TSPIA NAADNFLGEVLFWLGVKEFDPRGKAAVQ
Sbjct: 181 AFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQ 240

Query: 241 LLVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTI 300
           LLV+VCAKPGVDCINLLTSFTGQNCCLNPSV+Q FL HEPQPTAT+NMIHL+QMIRSGTI
Sbjct: 241 LLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTI 300

Query: 301 AMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDH 360
           AMYDYVDVIEN+KHYGQ TPP YNMTSIPNDFPLFLSYGGADALSDVNDVQLLL++ KDH
Sbjct: 301 AMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDH 360

Query: 361 DGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           DGDKLVVQFREDYAHADFVMGENAKQVVYDPL+AF  LQ
Sbjct: 361 DGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 395

BLAST of MC02g0587 vs. NCBI nr
Match: KAA0054492.1 (triacylglycerol lipase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 711 bits (1834), Expect = 7.06e-257
Identity = 347/399 (86.97%), Postives = 371/399 (92.98%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           M SPN F SIVLLLLFYG A GI+RVSALEPGSV+GICKSLVEPHD+ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVLLLLFYGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRG 120
           ILSMQRIPSGRT+SANGPPVLLQHGLLMDA TWLMLPPESSLAF+LADKGFDVWLANTRG
Sbjct: 61  ILSMQRIPSGRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTRG 120

Query: 121 TKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAA 180
           TKFSQGH+SLGPDDP FWDWSWDELVA DLPATLQYVH+HTGQKMHYVGHSLGTLIALAA
Sbjct: 121 TKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLIALAA 180

Query: 181 FSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQL 240
           FSKHQLL+MLRSAALISPIAHLGK+TSPIA NAADNFLGE+  +  +       KA VQL
Sbjct: 181 FSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEIFTYFKLTN----RKAGVQL 240

Query: 241 LVDVCAKPGVDCINLLTSFTG-QNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTI 300
           LVDVCAKPGVDC+NLLTSFTG QNCCLNPSV+QIFLTHEPQPTAT+NMIHL+QMIRSGTI
Sbjct: 241 LVDVCAKPGVDCVNLLTSFTGCQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTI 300

Query: 301 AMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDH 360
           +MYDYVDVI+N+KHYGQPTPPEYNM SIP DFPLFL+YGGADALSDVNDVQLLL++LKDH
Sbjct: 301 SMYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDH 360

Query: 361 DGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           DGDKLVVQFREDYAHADFVMG+NAKQ VYDPL+AF  LQ
Sbjct: 361 DGDKLVVQFREDYAHADFVMGKNAKQAVYDPLIAFFNLQ 395

BLAST of MC02g0587 vs. ExPASy TrEMBL
Match: A0A5D3CTL1 (Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold552G00300 PE=3 SV=1)

HSP 1 Score: 752 bits (1942), Expect = 1.34e-273
Identity = 361/398 (90.70%), Postives = 382/398 (95.98%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           M SPN F SIVLLLLFYG A GI+RVSALEPGSV+GICKSLVEPHD+ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVLLLLFYGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRG 120
           ILSMQRIPSGRT+SANGPPVLLQHGLLMDA TWLMLPPESSLAF+LADKGFDVWLANTRG
Sbjct: 61  ILSMQRIPSGRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTRG 120

Query: 121 TKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAA 180
           TKFSQGH+SLGPDDP FWDWSWDELVA DLPATLQYVH+HTGQKMHYVGHSLGTLIALAA
Sbjct: 121 TKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLIALAA 180

Query: 181 FSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQL 240
           FSKHQLL+MLRSAALISPIAHLGK+TSPIA NAADNFLGEVLFWLGVKEFDPRGKA VQL
Sbjct: 181 FSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGVQL 240

Query: 241 LVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTIA 300
           LVDVCAKPGVDC+NLLTSFTGQNCCLNPSV+QIFLTHEPQPTAT+NMIHL+QMIRSGTI+
Sbjct: 241 LVDVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTIS 300

Query: 301 MYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHD 360
           MYDYVDVI+N+KHYGQPTPPEYNM SIP DFPLFL+YGGADALSDVNDVQLLL++LKDHD
Sbjct: 301 MYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDHD 360

Query: 361 GDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           GDKLVVQFREDYAHADFVMG+NAKQ VYDPL+AF  LQ
Sbjct: 361 GDKLVVQFREDYAHADFVMGKNAKQAVYDPLIAFFNLQ 398

BLAST of MC02g0587 vs. ExPASy TrEMBL
Match: A0A1S3C4E7 (Lipase OS=Cucumis melo OX=3656 GN=LOC103496348 PE=3 SV=1)

HSP 1 Score: 752 bits (1942), Expect = 1.34e-273
Identity = 361/398 (90.70%), Postives = 382/398 (95.98%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           M SPN F SIVLLLLFYG A GI+RVSALEPGSV+GICKSLVEPHD+ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVLLLLFYGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRG 120
           ILSMQRIPSGRT+SANGPPVLLQHGLLMDA TWLMLPPESSLAF+LADKGFDVWLANTRG
Sbjct: 61  ILSMQRIPSGRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTRG 120

Query: 121 TKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAA 180
           TKFSQGH+SLGPDDP FWDWSWDELVA DLPATLQYVH+HTGQKMHYVGHSLGTLIALAA
Sbjct: 121 TKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLIALAA 180

Query: 181 FSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQL 240
           FSKHQLL+MLRSAALISPIAHLGK+TSPIA NAADNFLGEVLFWLGVKEFDPRGKA VQL
Sbjct: 181 FSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGVQL 240

Query: 241 LVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTIA 300
           LVDVCAKPGVDC+NLLTSFTGQNCCLNPSV+QIFLTHEPQPTAT+NMIHL+QMIRSGTI+
Sbjct: 241 LVDVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTIS 300

Query: 301 MYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHD 360
           MYDYVDVI+N+KHYGQPTPPEYNM SIP DFPLFL+YGGADALSDVNDVQLLL++LKDHD
Sbjct: 301 MYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDHD 360

Query: 361 GDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           GDKLVVQFREDYAHADFVMG+NAKQ VYDPL+AF  LQ
Sbjct: 361 GDKLVVQFREDYAHADFVMGKNAKQAVYDPLIAFFNLQ 398

BLAST of MC02g0587 vs. ExPASy TrEMBL
Match: A0A0A0KF57 (Lipase OS=Cucumis sativus OX=3659 GN=Csa_6G168240 PE=3 SV=1)

HSP 1 Score: 739 bits (1909), Expect = 1.43e-268
Identity = 355/398 (89.20%), Postives = 377/398 (94.72%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           M SPN F SIV  LLF G A GI+RVSALEPGSV+GICKSLVEPHD+ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVFSLLFCGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRG 120
           ILSMQRIPS RT+SANGPPVLLQHGLLMDA TWLMLPPESSLAF+LADKGFDVWLANTRG
Sbjct: 61  ILSMQRIPSRRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTRG 120

Query: 121 TKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAA 180
           TKFSQGH+SLGPDDP FWDWSWDELVA DLPATLQYVH+HTGQKMHYVGHSLGTL ALAA
Sbjct: 121 TKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLTALAA 180

Query: 181 FSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQL 240
           FSKHQLL+MLRSAALISPIAHLGK+TSPIA NAADNFLGEVLFWLGVKEFDPRGKA +QL
Sbjct: 181 FSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGIQL 240

Query: 241 LVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTIA 300
           LV+VCAKPGVDC+NLLTSFTGQNCCLNPSV+QIFLTHEPQPTAT+NMIHL+QMIRSGTI+
Sbjct: 241 LVEVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTIS 300

Query: 301 MYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHD 360
           MYDYVDVI+N+KHYGQPTPPEYNM SIP DFPLFL+YGGADALSDVNDVQLLL++LKDHD
Sbjct: 301 MYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDHD 360

Query: 361 GDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           GDKLVVQFREDYAHADFVMGENAKQ VYDPL+AF  LQ
Sbjct: 361 GDKLVVQFREDYAHADFVMGENAKQAVYDPLIAFFNLQ 398

BLAST of MC02g0587 vs. ExPASy TrEMBL
Match: A0A5A7UFE9 (Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G002710 PE=3 SV=1)

HSP 1 Score: 711 bits (1834), Expect = 3.42e-257
Identity = 347/399 (86.97%), Postives = 371/399 (92.98%), Query Frame = 0

Query: 1   MNSPNPFTSIVLLLLFYGSAFGIRRVSALEPGSVDGICKSLVEPHDYACEEHLVITNDGF 60
           M SPN F SIVLLLLFYG A GI+RVSALEPGSV+GICKSLVEPHD+ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVLLLLFYGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDVWLANTRG 120
           ILSMQRIPSGRT+SANGPPVLLQHGLLMDA TWLMLPPESSLAF+LADKGFDVWLANTRG
Sbjct: 61  ILSMQRIPSGRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTRG 120

Query: 121 TKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLGTLIALAA 180
           TKFSQGH+SLGPDDP FWDWSWDELVA DLPATLQYVH+HTGQKMHYVGHSLGTLIALAA
Sbjct: 121 TKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLIALAA 180

Query: 181 FSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPRGKAAVQL 240
           FSKHQLL+MLRSAALISPIAHLGK+TSPIA NAADNFLGE+  +  +       KA VQL
Sbjct: 181 FSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEIFTYFKLTN----RKAGVQL 240

Query: 241 LVDVCAKPGVDCINLLTSFTG-QNCCLNPSVTQIFLTHEPQPTATRNMIHLAQMIRSGTI 300
           LVDVCAKPGVDC+NLLTSFTG QNCCLNPSV+QIFLTHEPQPTAT+NMIHL+QMIRSGTI
Sbjct: 241 LVDVCAKPGVDCVNLLTSFTGCQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTI 300

Query: 301 AMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDH 360
           +MYDYVDVI+N+KHYGQPTPPEYNM SIP DFPLFL+YGGADALSDVNDVQLLL++LKDH
Sbjct: 301 SMYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDH 360

Query: 361 DGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           DGDKLVVQFREDYAHADFVMG+NAKQ VYDPL+AF  LQ
Sbjct: 361 DGDKLVVQFREDYAHADFVMGKNAKQAVYDPLIAFFNLQ 395

BLAST of MC02g0587 vs. ExPASy TrEMBL
Match: A0A6J1D7I1 (triacylglycerol lipase 2-like OS=Momordica charantia OX=3673 GN=LOC111018074 PE=4 SV=1)

HSP 1 Score: 704 bits (1818), Expect = 5.10e-253
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 54  VITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDV 113
           VITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDV
Sbjct: 159 VITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLPPESSLAFILADKGFDV 218

Query: 114 WLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLG 173
           WLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLG
Sbjct: 219 WLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYVHNHTGQKMHYVGHSLG 278

Query: 174 TLIALAAFSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPR 233
           TLIALAAFSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPR
Sbjct: 279 TLIALAAFSKHQLLNMLRSAALISPIAHLGKITSPIAINAADNFLGEVLFWLGVKEFDPR 338

Query: 234 GKAAVQLLVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQM 293
           GKAAVQLLVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQM
Sbjct: 339 GKAAVQLLVDVCAKPGVDCINLLTSFTGQNCCLNPSVTQIFLTHEPQPTATRNMIHLAQM 398

Query: 294 IRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLL 353
           IRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLL
Sbjct: 399 IRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLL 458

Query: 354 NSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 398
           NSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ
Sbjct: 459 NSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLMAFLKLQ 503

BLAST of MC02g0587 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 405.6 bits (1041), Expect = 4.6e-113
Identity = 189/368 (51.36%), Postives = 255/368 (69.29%), Query Frame = 0

Query: 36  GICKSLVEPHDYACEEHLVITNDGFILSMQRIPSGRTSSANG-----PPVLLQHGLLMDA 95
           GIC S V    Y CEEH V+T DG+IL+MQRIP GR  +  G      PVL+QHG+L+D 
Sbjct: 50  GICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDG 109

Query: 96  VTWLMLPPESSLAFILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDL 155
           ++WL+ P + +L  ILAD+GFDVW+ NTRGT+FS+ H  L P   +FW+W+WDELV+ DL
Sbjct: 110 MSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDL 169

Query: 156 PATLQYVHNHTGQKMHYVGHSLGTLIALAAFSKHQLLNMLRSAALISPIAHLGKITSPIA 215
           PA   ++H  TGQK+HY+GHSLGTLI  A+FS+  L++ +RSAA++SP+A+L  +T+ I 
Sbjct: 170 PAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIG 229

Query: 216 INAADNFLGEVLFWLGVKEFDPRGKAAVQLLVDVCAKPGVDCINLLTSFTGQNCCLNPSV 275
             AA  FL E    LG  EF+P+       +  +C K G+DC +L++  TG+NCCLN S 
Sbjct: 230 DIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAST 289

Query: 276 TQIFLTHEPQPTATRNMIHLAQMIRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIPND 335
             +FL +EPQ T+T+NMIHLAQ +R   +  Y+Y     N+KHYGQ  PP YN+++IP++
Sbjct: 290 IDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHE 349

Query: 336 FPLFLSYGGADALSDVNDVQLLLNSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDP 395
            PLF SYGG D+L+DV DV+ LL+  K HD DK+ VQF +DYAHADF+MG  AK VVY+ 
Sbjct: 350 LPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQ 409

Query: 396 LMAFLKLQ 399
           +  F K Q
Sbjct: 410 VATFFKRQ 417

BLAST of MC02g0587 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 313.2 bits (801), Expect = 3.1e-85
Identity = 149/368 (40.49%), Postives = 225/368 (61.14%), Query Frame = 0

Query: 29  LEPGSVDGICKSLVEPHDYACEEHLVITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLM 88
           L    V+ +C  L+ P +Y+C EH + T DG+IL++QR+ S      +GPPVLLQHGL M
Sbjct: 24  LHGSPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQSGPPVLLQHGLFM 83

Query: 89  DAVTWLMLPPESSLAFILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVAL 148
               W +  P+ SL FILAD GFDVW+ N RGT++S GH +L   D  FWDWSW +L   
Sbjct: 84  AGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMY 143

Query: 149 DLPATLQYVHNHTGQKMHYVGHSLGTLIALAAFSKHQLLNMLRSAALISPIAHLGKITSP 208
           DL   +QY+++ +  K+  VGHS GT+++ AA ++  +  M+ +AAL+ PI++L  +T+P
Sbjct: 144 DLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAP 203

Query: 209 IAINAADNFLGEVLFWLGVKEFDPRGKAAVQLLVDVCAKPGVDCINLLTSFTGQNCCLNP 268
           +        L +++  LG+ + + R    V+L+  +C +  +DC + LTS TG NCC N 
Sbjct: 204 LVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLC-EGHMDCTDFLTSITGTNCCFNA 263

Query: 269 SVTQIFLTHEPQPTATRNMIHLAQMIRSGTIAMYDYVDVIENMKHYGQPTPPEYNMTSIP 328
           S  + +L +EP P++ +N+ HL QMIR GT A YDY    +N++ YG   PPE+ ++ IP
Sbjct: 264 SKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDY-GYFKNLRTYGLSKPPEFILSHIP 323

Query: 329 NDFPLFLSYGGADALSDVNDVQLLLNSLKDHDGDKLVVQFREDYAHADFVMGENAKQVVY 388
              P+++ YGG D L+DV DV+  L  L         + + EDY H DFV+G +AK+ VY
Sbjct: 324 ASLPMWMGYGGTDGLADVTDVEHTLAELPSSP----ELLYLEDYGHIDFVLGSSAKEDVY 383

Query: 389 DPLMAFLK 397
             ++ F +
Sbjct: 384 KHMIQFFR 385

BLAST of MC02g0587 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 1.6e-17
Identity = 99/385 (25.71%), Postives = 156/385 (40.52%), Query Frame = 0

Query: 38  CKSLVEPHDYACEEHLVITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLP 97
           C+ ++    Y  E   VIT+DG++L ++RIP      A    V LQHG+L  ++ W+   
Sbjct: 296 CQDVITELGYPYEAIRVITSDGYVLVLERIPRRDARKA----VFLQHGVLDSSMGWVSNG 355

Query: 98  PESSLAFILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYV 157
              S AF   D+G+DV+L N RG   S+ H +       FW +S +E    D+PA ++ +
Sbjct: 356 VVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEKI 415

Query: 158 H-------------------NHTGQKMHYVGHSLGTLIALAAFSKHQLL---NMLRSAAL 217
           H                        K+  + HSLG    L      ++    + L    L
Sbjct: 416 HEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRLSRLIL 475

Query: 218 ISPIA-HLGKITSPIAINAADNFLGEVL------FWLGVKEFDPRGKAAVQLLVDVCAKP 277
           +SP   H         +     F+  VL      F++  + F        +   +  A  
Sbjct: 476 LSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLARDFHNYPALG 535

Query: 278 GVDCINLLTSFTGQNCCLNPSVTQIFLTH----EPQPTATRNMIHLAQMIRSGTIAMYDY 337
           G+  +  L S+       N  V  + L H    +    + R   HLAQ+  +G   MYDY
Sbjct: 536 GL--VQTLMSYVVGGDSSN-WVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRMYDY 595

Query: 338 VDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHDGDKL 389
                NM+ YG P P +   +    D P+ L  G  D +   + V+   N ++D + D  
Sbjct: 596 GSRSANMEVYGSPEPLDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAEVD-- 655

BLAST of MC02g0587 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 1.6e-17
Identity = 99/385 (25.71%), Postives = 156/385 (40.52%), Query Frame = 0

Query: 38  CKSLVEPHDYACEEHLVITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLP 97
           C+ ++    Y  E   VIT+DG++L ++RIP      A    V LQHG+L  ++ W+   
Sbjct: 227 CQDVITELGYPYEAIRVITSDGYVLVLERIPRRDARKA----VFLQHGVLDSSMGWVSNG 286

Query: 98  PESSLAFILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYV 157
              S AF   D+G+DV+L N RG   S+ H +       FW +S +E    D+PA ++ +
Sbjct: 287 VVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEKI 346

Query: 158 H-------------------NHTGQKMHYVGHSLGTLIALAAFSKHQLL---NMLRSAAL 217
           H                        K+  + HSLG    L      ++    + L    L
Sbjct: 347 HEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRLSRLIL 406

Query: 218 ISPIA-HLGKITSPIAINAADNFLGEVL------FWLGVKEFDPRGKAAVQLLVDVCAKP 277
           +SP   H         +     F+  VL      F++  + F        +   +  A  
Sbjct: 407 LSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLARDFHNYPALG 466

Query: 278 GVDCINLLTSFTGQNCCLNPSVTQIFLTH----EPQPTATRNMIHLAQMIRSGTIAMYDY 337
           G+  +  L S+       N  V  + L H    +    + R   HLAQ+  +G   MYDY
Sbjct: 467 GL--VQTLMSYVVGGDSSN-WVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRMYDY 526

Query: 338 VDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHDGDKL 389
                NM+ YG P P +   +    D P+ L  G  D +   + V+   N ++D + D  
Sbjct: 527 GSRSANMEVYGSPEPLDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAEVD-- 586

BLAST of MC02g0587 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 8.1e-17
Identity = 97/384 (25.26%), Postives = 152/384 (39.58%), Query Frame = 0

Query: 38  CKSLVEPHDYACEEHLVITNDGFILSMQRIPSGRTSSANGPPVLLQHGLLMDAVTWLMLP 97
           C+ ++    Y  E   V+T+DG+ L ++RIP      A    V LQHG++  ++ W+   
Sbjct: 294 CQDVITELGYPYEAIRVVTSDGYGLLLERIPRRDARKA----VYLQHGVMDSSMGWVSNG 353

Query: 98  PESSLAFILADKGFDVWLANTRGTKFSQGHTSLGPDDPSFWDWSWDELVALDLPATLQYV 157
              S AF   D+G+DV+L N RG   S+ H         FW +S +E    D+PA ++ +
Sbjct: 354 VVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVKKNISSKDFWRYSINEHATEDIPAMIEKI 413

Query: 158 H-------------------NHTGQKMHYVGHSLG---TLIALAAFSKHQLLNMLRSAAL 217
           H                        K+  V HSLG    L+ +      +  + L    L
Sbjct: 414 HEIKTSELKLYQPTMEEVVNEDQPYKLCVVSHSLGGAAVLMYVITRKIEEKPHRLSRLIL 473

Query: 218 ISPIA-HLGKITSPIAINAADNFLGEVL------FWLGVKEFDPRGKAAVQLLVDVCAKP 277
           +SP   H         +     FLG VL      F++  K F        +L  D    P
Sbjct: 474 LSPAGFHYDSNMCFTLMEYTFLFLGPVLSRIVPAFYIPTKFF---RMLLNKLARDFHNYP 533

Query: 278 GVDCI--NLLTSFTGQNCCLNPSVTQI--FLTHEPQPTATRNMIHLAQMIRSGTIAMYDY 337
            V  +   L++   G +      V  +  +  ++    + R   HLAQ+  SG   M+DY
Sbjct: 534 AVGGLVQTLMSYVVGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQIKHSGKFKMFDY 593

Query: 338 VDVIENMKHYGQPTPPEYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLNSLKDHDGDKL 389
                NM  YG P P +        D P+ L  G  D +   + V+     ++D   D  
Sbjct: 594 GSSSANMDVYGSPEPLDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKHYRVMRDSGVDVS 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q67ZU16.5e-11251.36Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1[more]
Q71DJ54.4e-8440.49Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
P046348.3e-5935.52Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1[more]
P800352.4e-5837.40Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV... [more]
Q9CPP72.0e-5737.71Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038901645.11.05e-27792.21triacylglycerol lipase 2-like isoform X1 [Benincasa hispida][more]
XP_008456397.12.76e-27390.70PREDICTED: triacylglycerol lipase 2-like [Cucumis melo] >TYK14548.1 triacylglyce... [more]
KGN47007.12.95e-26889.20hypothetical protein Csa_020982 [Cucumis sativus][more]
KAG6604747.15.68e-26389.72Triacylglycerol lipase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG70... [more]
KAA0054492.17.06e-25786.97triacylglycerol lipase 2-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A5D3CTL11.34e-27390.70Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold552G00300 PE=3 SV... [more]
A0A1S3C4E71.34e-27390.70Lipase OS=Cucumis melo OX=3656 GN=LOC103496348 PE=3 SV=1[more]
A0A0A0KF571.43e-26889.20Lipase OS=Cucumis sativus OX=3659 GN=Csa_6G168240 PE=3 SV=1[more]
A0A5A7UFE93.42e-25786.97Lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G002710 PE=3 SV... [more]
A0A6J1D7I15.10e-253100.00triacylglycerol lipase 2-like OS=Momordica charantia OX=3673 GN=LOC111018074 PE=... [more]
Match NameE-valueIdentityDescription
AT5G14180.14.6e-11351.36Myzus persicae-induced lipase 1 [more]
AT2G15230.13.1e-8540.49lipase 1 [more]
AT1G73920.11.6e-1725.71alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.21.6e-1725.71alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.18.1e-1725.26alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 41..95
e-value: 8.1E-19
score: 67.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 32..397
e-value: 1.3E-119
score: 401.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 41..396
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 102..358
e-value: 3.6E-7
score: 29.7
IPR025483Lipase, eukaryoticPIRSFPIRSF000862Steryl_ester_lipcoord: 7..398
e-value: 4.6E-120
score: 399.1
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 9..398
NoneNo IPR availablePANTHERPTHR11005:SF128LIPASEcoord: 9..398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0587.1MC02g0587.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016788 hydrolase activity, acting on ester bonds