MC02g0571 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0571
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionseed biotin-containing protein SBP65-like
LocationMC02: 4597164 .. 4599797 (+)
RNA-Seq ExpressionMC02g0571
SyntenyMC02g0571
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACGGAGAAGCTGAGCCGCAGAGACGACACGACGGCGGAGAGGGAGGGTCGGGTGGAGAAAGAAAGAGTCCCCCAATTGACCAGCCACTTTGAGGCCATAGCGATCCAGGGCAAACCCACTGACCCCACCAATGCCAAAGCCTCAATTTTACAGAAAACAGACGACACACAGAAGAGTGCCGGGAAAGATGCCCATGGAGTGGGAAAGAGCTCCGACACCAGCATTGGGAAGGATGAGGAGAGGCAAAGAGTTTCAGGTGGAGGTGGAAAATCAGAAGAAACCCATAAATTGGCTGCCCAATTCGAGTCTCTTGCTGACAAAGTGAGAGACAGGAGAGAGACGGATACAGAGAATGAAAGGGAGAGACAAGCACGTGCTGATAAACAGAGACAAGAAACAGGGGAACAACAACGAGTCTCGCAACAGACGAAAGGGAAAGAAGGTGAGATGGCTTCTGCTCAGAACCAACCATCCCTCGAGGAAATTTCCAATTACAGAGCAATAGCACAGCAGAAGTCAAATGAGGCAATAATGGCTGCCAGGGAACGCTACGACAAAGCCAACCAAGAGTCACTCAACAAAGAAGCTGCGAAACAGAGAGGCACGGAAGGCAAAGAAACCACTAAAGAGGGAGGCCAAACGGGTTTAGAGACGGTGAAAGAGACGCTAACGAGTGCAGCCCAAACGGCGAAGGAATACACCGCCCCAGTAGCGGAGAAGGCTAAAGACTACACAGTCTCCGCGGGCGAAACAACAGCTCATTACCTGGGAGAGAAGGCGGTGGCAGCCAAGGATGCCACTATAGAAAGCGGGAAAGTAGCGGCTGAATATGCAGGCAAGGCGGCAGGGGATTTGAAGGATAAGGCCACCGTGGCAGGGTGGAGCACGGCGCATTACACCTGCGACACCGCGGTGGAGGGGACAAAAGCAGCAGCTCGGCTCGTGAAGGGAGCAGCAGAGTATGCCGGAGCCATTGCGGCCAAGCCTCTATCTGCGGCAAAGAATGTAGCTGAATCAACAGGGGAGAGCATGAAGGACTACACAGCTAGAAAGAAAGAAGAAGCACAGAGAGAATCGATGCAAACACAGGCTGAGAAAGAATCAATGTATGAGAAAACAAAAGAAAGTTTTGAAGGGGCGAGAGAAGAAGTGAAGGAAAGGGGAAGAGGAGTAGGATATGAGAAGAGAGGGGAAGAGGGGAGAGCGGGTGATGGTGGGTTATTGGGGGCGATTGGGGAGACCATATATGAGATTGCACAGACAACCAAAGATTTAGTGATTGGGCCTGGTGATGAGACTGGAAAACAAAGCACGTTAGGGTTTGATCTTGGGTTAGGCGAGGAGGAAGAAGCAAGAGGCCAAGGATATGAGAAGAAAGAGCACTATGAAGCTGCTTCCAGAAAGGAACACAAGGCATGATGAAGCAGAGTTATGGGCTGTGTTTATGTGAATTTCTGAGTTTTTCCTTTTGTTTTAAATCCTCAAAAAGGGTTGGGAAGAATGGGAATAGTTTTGAAGTTCTGTTTCATCAGGGTGAATAAAATTTTGAGCATGTGTATCAGTTTTCTACTATGTTCTAAGAATCAATAACTCCAAACCGCTAAAATACAGTTTCTGCTACTCTTATCACATCACATCAGGCACACCATATAAATTTTGAACATCTTTTCTGTACACTATCAAAGTTTGCAGACTTTATTTTAAGAAAGAAAGCTACAAATTAATTCCGACACATGATAGGTCTACAATTCATGACTGTGAGGCAACTGCAACTACAGAATTGTGCCAAAACAAAGAATCCGATAGAATGTAACATGCTGAGTGGAGCATGAGTAGAGAAAGAAAGCAAGCAAGCAAGCAATGCCATATTCGGTACGCCAAAACTACCGCCATCTAATTTCTAATCGGATTCTACCATATGTTACAAATTATTACAATGGAGGCAATCCTCCCATAACAAGAGAGACTAAATCTAAGCATTCTATCATCGCCTAATAGACCACCCTTAGTTCGCATTCAGGACTGCTTGAAGGTACCTTCACAACATTTTCACTGTACACCAGCTGCAGATTCAAACCCACCATCTGAAGTCTTCCTCTAGGCATCAATCTACCTTCAAATCCATAAATATCAAGCAATCGTTATTCAACCTACTAGATAACGAACACACTTTTTAAGTGCAACACTGTCAACTGTGAGAAATATAAATGGGAGTGCAACTAAAATATAAACAAGTCTTCACATTATATACTAAATGGGATCTACGGACGAGTTTCTGGTGTCAGGCTACTATTTCCACATTAAACTTCAATCAAGTTATTAATTGTTGTAAATTTGGGCATTGTCTTAAAACTAAATAACTGATGTTCAAGATGATAAAAGAGTAGAAACAGTGAACAATTGATACACAACATGCTACGCTACAGAGATGCAATTATTCAGCATCGGATTTGCACAACAAAAGGCGGATATAAGAACAGTATTTATGCTCCATCGAAGAAAAGAAGTAACATGTAAATAGAATCGCATCAAGAAATACTTCTCCTCCAAAAACACAATAGAAAATGCCAAATAGTACGTTAAAAATCAAGCACCTAAACGCTAAAACAATAACCTCAGCAGTCAGCA

mRNA sequence

ATGGCGACGGAGAAGCTGAGCCGCAGAGACGACACGACGGCGGAGAGGGAGGGTCGGGTGGAGAAAGAAAGAGTCCCCCAATTGACCAGCCACTTTGAGGCCATAGCGATCCAGGGCAAACCCACTGACCCCACCAATGCCAAAGCCTCAATTTTACAGAAAACAGACGACACACAGAAGAGTGCCGGGAAAGATGCCCATGGAGTGGGAAAGAGCTCCGACACCAGCATTGGGAAGGATGAGGAGAGGCAAAGAGTTTCAGGTGGAGGTGGAAAATCAGAAGAAACCCATAAATTGGCTGCCCAATTCGAGTCTCTTGCTGACAAAGTGAGAGACAGGAGAGAGACGGATACAGAGAATGAAAGGGAGAGACAAGCACGTGCTGATAAACAGAGACAAGAAACAGGGGAACAACAACGAGTCTCGCAACAGACGAAAGGGAAAGAAGGTGAGATGGCTTCTGCTCAGAACCAACCATCCCTCGAGGAAATTTCCAATTACAGAGCAATAGCACAGCAGAAGTCAAATGAGGCAATAATGGCTGCCAGGGAACGCTACGACAAAGCCAACCAAGAGTCACTCAACAAAGAAGCTGCGAAACAGAGAGGCACGGAAGGCAAAGAAACCACTAAAGAGGGAGGCCAAACGGGTTTAGAGACGGTGAAAGAGACGCTAACGAGTGCAGCCCAAACGGCGAAGGAATACACCGCCCCAGTAGCGGAGAAGGCTAAAGACTACACAGTCTCCGCGGGCGAAACAACAGCTCATTACCTGGGAGAGAAGGCGGTGGCAGCCAAGGATGCCACTATAGAAAGCGGGAAAGTAGCGGCTGAATATGCAGGCAAGGCGGCAGGGGATTTGAAGGATAAGGCCACCGTGGCAGGGTGGAGCACGGCGCATTACACCTGCGACACCGCGGTGGAGGGGACAAAAGCAGCAGCTCGGCTCGTGAAGGGAGCAGCAGAGTATGCCGGAGCCATTGCGGCCAAGCCTCTATCTGCGGCAAAGAATGTAGCTGAATCAACAGGGGAGAGCATGAAGGACTACACAGCTAGAAAGAAAGAAGAAGCACAGAGAGAATCGATGCAAACACAGGCTGAGAAAGAATCAATGTATGAGAAAACAAAAGAAAGTTTTGAAGGGGCGAGAGAAGAAGTGAAGGAAAGGGGAAGAGGAGTAGGATATGAGAAGAGAGGGGAAGAGGGGAGAGCGGGTGATGGTGGGTTATTGGGGGCGATTGGGGAGACCATATATGAGATTGCACAGACAACCAAAGATTTAGTGATTGGGCCTGGTGATGAGACTGGAAAACAAAGCACGTTAGGGTTTGATCTTGGGTTAGGCGAGGAGGAAGAAGCAAGAGGCCAAGGATATGAGAAGAAAGAGCACTATGAAGCTGCTTCCAGAAAGGAACACAAGGCATGATGAAGCAGAGTTATGGGCTGTGTTTATGTGAATTTCTGAGTTTTTCCTTTTGTTTTAAATCCTCAAAAAGGGTTGGGAAGAATGGGAATAGTTTTGAAGTTCTGTTTCATCAGGGTGAATAAAATTTTGAGCATGTGTATCAGTTTTCTACTATGTTCTAAGAATCAATAACTCCAAACCGCTAAAATACAGTTTCTGCTACTCTTATCACATCACATCAGGCACACCATATAAATTTTGAACATCTTTTCTGTACACTATCAAAGTTTGCAGACTTTATTTTAAGAAAGAAAGCTACAAATTAATTCCGACACATGATAGGTCTACAATTCATGACTGTGAGGCAACTGCAACTACAGAATTGTGCCAAAACAAAGAATCCGATAGAATGTAACATGCTGAGTGGAGCATGAGTAGAGAAAGAAAGCAAGCAAGCAAGCAATGCCATATTCGGTACGCCAAAACTACCGCCATCTAATTTCTAATCGGATTCTACCATATGTTACAAATTATTACAATGGAGGCAATCCTCCCATAACAAGAGAGACTAAATCTAAGCATTCTATCATCGCCTAATAGACCACCCTTAGTTCGCATTCAGGACTGCTTGAAGGTACCTTCACAACATTTTCACTGTACACCAGCTGCAGATTCAAACCCACCATCTGAAGTCTTCCTCTAGGCATCAATCTACCTTCAAATCCATAAATATCAAGCAATCGTTATTCAACCTACTAGATAACGAACACACTTTTTAAGTGCAACACTGTCAACTGTGAGAAATATAAATGGGAGTGCAACTAAAATATAAACAAGTCTTCACATTATATACTAAATGGGATCTACGGACGAGTTTCTGGTGTCAGGCTACTATTTCCACATTAAACTTCAATCAAGTTATTAATTGTTGTAAATTTGGGCATTGTCTTAAAACTAAATAACTGATGTTCAAGATGATAAAAGAGTAGAAACAGTGAACAATTGATACACAACATGCTACGCTACAGAGATGCAATTATTCAGCATCGGATTTGCACAACAAAAGGCGGATATAAGAACAGTATTTATGCTCCATCGAAGAAAAGAAGTAACATGTAAATAGAATCGCATCAAGAAATACTTCTCCTCCAAAAACACAATAGAAAATGCCAAATAGTACGTTAAAAATCAAGCACCTAAACGCTAAAACAATAACCTCAGCAGTCAGCA

Coding sequence (CDS)

ATGGCGACGGAGAAGCTGAGCCGCAGAGACGACACGACGGCGGAGAGGGAGGGTCGGGTGGAGAAAGAAAGAGTCCCCCAATTGACCAGCCACTTTGAGGCCATAGCGATCCAGGGCAAACCCACTGACCCCACCAATGCCAAAGCCTCAATTTTACAGAAAACAGACGACACACAGAAGAGTGCCGGGAAAGATGCCCATGGAGTGGGAAAGAGCTCCGACACCAGCATTGGGAAGGATGAGGAGAGGCAAAGAGTTTCAGGTGGAGGTGGAAAATCAGAAGAAACCCATAAATTGGCTGCCCAATTCGAGTCTCTTGCTGACAAAGTGAGAGACAGGAGAGAGACGGATACAGAGAATGAAAGGGAGAGACAAGCACGTGCTGATAAACAGAGACAAGAAACAGGGGAACAACAACGAGTCTCGCAACAGACGAAAGGGAAAGAAGGTGAGATGGCTTCTGCTCAGAACCAACCATCCCTCGAGGAAATTTCCAATTACAGAGCAATAGCACAGCAGAAGTCAAATGAGGCAATAATGGCTGCCAGGGAACGCTACGACAAAGCCAACCAAGAGTCACTCAACAAAGAAGCTGCGAAACAGAGAGGCACGGAAGGCAAAGAAACCACTAAAGAGGGAGGCCAAACGGGTTTAGAGACGGTGAAAGAGACGCTAACGAGTGCAGCCCAAACGGCGAAGGAATACACCGCCCCAGTAGCGGAGAAGGCTAAAGACTACACAGTCTCCGCGGGCGAAACAACAGCTCATTACCTGGGAGAGAAGGCGGTGGCAGCCAAGGATGCCACTATAGAAAGCGGGAAAGTAGCGGCTGAATATGCAGGCAAGGCGGCAGGGGATTTGAAGGATAAGGCCACCGTGGCAGGGTGGAGCACGGCGCATTACACCTGCGACACCGCGGTGGAGGGGACAAAAGCAGCAGCTCGGCTCGTGAAGGGAGCAGCAGAGTATGCCGGAGCCATTGCGGCCAAGCCTCTATCTGCGGCAAAGAATGTAGCTGAATCAACAGGGGAGAGCATGAAGGACTACACAGCTAGAAAGAAAGAAGAAGCACAGAGAGAATCGATGCAAACACAGGCTGAGAAAGAATCAATGTATGAGAAAACAAAAGAAAGTTTTGAAGGGGCGAGAGAAGAAGTGAAGGAAAGGGGAAGAGGAGTAGGATATGAGAAGAGAGGGGAAGAGGGGAGAGCGGGTGATGGTGGGTTATTGGGGGCGATTGGGGAGACCATATATGAGATTGCACAGACAACCAAAGATTTAGTGATTGGGCCTGGTGATGAGACTGGAAAACAAAGCACGTTAGGGTTTGATCTTGGGTTAGGCGAGGAGGAAGAAGCAAGAGGCCAAGGATATGAGAAGAAAGAGCACTATGAAGCTGCTTCCAGAAAGGAACACAAGGCATGA

Protein sequence

MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQKSAGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRETDTENERERQARADKQRQETGEQQRVSQQTKGKEGEMASAQNQPSLEEISNYRAIAQQKSNEAIMAARERYDKANQESLNKEAAKQRGTEGKETTKEGGQTGLETVKETLTSAAQTAKEYTAPVAEKAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAEYAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYEKRGEEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARGQGYEKKEHYEAASRKEHKA
Homology
BLAST of MC02g0571 vs. ExPASy Swiss-Prot
Match: Q39846 (Seed biotin-containing protein SBP65 OS=Glycine max OX=3847 GN=SBP65 PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.0e-47
Identity = 205/558 (36.74%), Postives = 275/558 (49.28%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MA+E+L+RR++TT E+E  VEK RVP++ +HFE +A Q K +D T  K       D  Q 
Sbjct: 1   MASEQLARRENTTTEKEIHVEKHRVPKMATHFEHLAEQAKESDITAGK-------DTPQG 60

Query: 61  SAGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRETD--- 120
           S   +A   G+      GK        GG GK+ ETH+L A FESLADKV D    +   
Sbjct: 61  SI--EALQAGERVKDHAGK---AMGDIGGRGKARETHELGAHFESLADKVTDHAAANVVG 120

Query: 121 ---------------------------------------------TENERER----QARA 180
                                                        TE ER R    Q  A
Sbjct: 121 NKESQREARGGVRDVGKFEMRTEGGEKGNKDRPELKTRTREVIGRTEKERGRESGGQVVA 180

Query: 181 DKQRQETGEQQRVSQQTKG---------------------KEGEMASAQNQPSLEEISNY 240
           +K R+    + RV  + +G                     +E E  S   + + E+ISNY
Sbjct: 181 EKGRETETARGRVGAENEGARTTAVITCTLEKGGGTQKPIREEERESESERSAWEQISNY 240

Query: 241 RAIAQQKSNEAIMAARERYDKANQ---ESLN---------KEAAKQRGTEGKETTKEGGQ 300
                  S++A    +E+Y++A Q   E+LN            AK    + K+ T E GQ
Sbjct: 241 -------SDQATQGVKEKYERAKQAASETLNTTTQTAQEKSAQAKNLAAQAKDATLEKGQ 300

Query: 301 TGLETVKETLTSAAQTAKEYTAPVAEKAKDYTV-------SAGETTAHYLGEKAVAAKDA 360
            G    K+T++SAA+TA E TAPVAEKAKDYT+       SAG TTA Y+GEKAV AKD 
Sbjct: 301 QGYAVTKDTISSAAKTASEKTAPVAEKAKDYTLQAAEKAKSAGGTTASYVGEKAVQAKDV 360

Query: 361 TIESGKVAAEYAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIA 420
            +ESGK AA YA K A DL+DKAT  GW+ AH++ +  VEGTKAAA +V+GAA YAG  A
Sbjct: 361 AVESGKSAAGYAAKVAADLRDKATAVGWAAAHFSAEKTVEGTKAAAHVVEGAAGYAGHKA 420

Query: 421 AKPLS----AAKNVAESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKE-SFEGAR 451
           A+  S    A K +A S GE+ K+YTA+KKEEAQRE    +A+K S  ++ +E   EG  
Sbjct: 421 AELASMSAGAVKGLAASAGETAKEYTAKKKEEAQRE---LEAKKPSQPQEAEERPSEGIG 480

BLAST of MC02g0571 vs. ExPASy Swiss-Prot
Match: Q41060 (Seed biotin-containing protein SBP65 OS=Pisum sativum OX=3888 GN=SBP65 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.1e-30
Identity = 173/554 (31.23%), Postives = 265/554 (47.83%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTD----PTNAKASILQKTD 60
           MA+E+LSRR++ T ER+ +  ++ VPQ T+HFE         +    P N   + L +  
Sbjct: 1   MASEQLSRRENITTERKIQNAEDSVPQRTTHFELRETHELGPNFQSLPRNENQAYLDRGA 60

Query: 61  DTQKSAGKDAHGVGKSS---DTSIGKDEERQRVSGGGGKSEETHKLAAQ--FESLADKVR 120
               SA      + ++    + +I +   R++   GG +     ++ ++   +SLA+   
Sbjct: 61  RAPLSANVSESYLDRARVPLNANIPEHRVREKEDFGGVRDMGKFQMESKGGNKSLAE--- 120

Query: 121 DRRETDTENERERQARADKQ-RQETGEQQRVSQQTKGKEGEMASAQNQPSLEEISNYRAI 180
           DR   DT   R R        ++ +G+ Q V ++ + +E  M   + + ++EEIS YR  
Sbjct: 121 DRETLDT---RSRMVTGTPHIKEASGKGQVVEERERARERAMEEEEKRLTMEEISKYRNQ 180

Query: 181 AQQKSNEAIMAARERYDKANQ---ESLNK--EAAKQRG--TEGKETTKEGGQTGLETVKE 240
           AQQ + EA+ AA+E+Y++A Q   E+L    +AA+++G   + K+ T E  Q G E   +
Sbjct: 181 AQQSALEALSAAQEKYERAKQATNETLRNTTQAAQEKGEAAQAKDATFEKTQQGYEMTGD 240

Query: 241 TLTSAAQTAKEYTAPVA-----------EKAKDYTVSAGETTAHY---LGEK----AVAA 300
           T++++A+TA E  A              E  +D   +A  T A Y     EK    AV A
Sbjct: 241 TVSNSARTASEKAAQAKNTTLGKTQQGYEATRDTVSNAARTAAEYATPAAEKARCVAVQA 300

Query: 301 KDATIESGKV-------AAEYAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVK 360
           KD T+E+GK        AAE A K A DLK+KATVAGW+ +HY     V+GT+AAA  V+
Sbjct: 301 KDVTLETGKTAAEKAKCAAEIAAKVAVDLKEKATVAGWTASHYATQLTVDGTRAAANAVE 360

Query: 361 GAAEY----AGAIAAKPLSAAKNVAESTGESMKDYTARKKEEAQRE-------------- 420
           GA  Y    A  +AAK +   K +A S GE+ K++TARKKEE+ RE              
Sbjct: 361 GAVGYVAPKASELAAKSVETVKGLAASAGETAKEFTARKKEESWREYEAKRASQLQEGEE 420

Query: 421 --------------SMQTQAEKESMYEKTK----------------------ESFEGARE 459
                           +TQA+  ++ EK +                      +  + A  
Sbjct: 421 ILPSTGGIGKVLPSGERTQAQGTNLQEKVQGKGSDILGAVTETVSDIGSSMIKPIDNANT 480

BLAST of MC02g0571 vs. ExPASy Swiss-Prot
Match: Q9SKP0 (Late embryogenesis abundant protein ECP63 OS=Arabidopsis thaliana OX=3702 GN=ECP63 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.7e-05
Identity = 78/239 (32.64%), Postives = 105/239 (43.93%), Query Frame = 0

Query: 196 KEAAKQRGTEGKETTKEGGQTGLE--------TVKETLTSAAQTA------KEYTAPVAE 255
           ++A   +  E  E+TKEG Q   E        TV++   +A  TA      K+YT   A+
Sbjct: 70  RDAVVGKTHEAAESTKEGAQIASEKAVGAKDATVEKAKETADYTAEKVGEYKDYTVDKAK 129

Query: 256 KAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAEYAGKAAGDLKDKATVAGWSTAHY 315
           +AKD T    + TA+Y  +KAV AKD T E      +YA   A + KDK       TA+Y
Sbjct: 130 EAKDTTAEKAKETANYTADKAVEAKDKTAEKIGEYKDYAVDKAVEAKDKTAEKAKETANY 189

Query: 316 TCDTAVEGTKAAAR--------LVKGAAEYAGAIAAKPLSAAKNVAESTGESMKDYTARK 375
           T D A E     A          V  A E     A K + A    AE TGE  KDYT  K
Sbjct: 190 TADKAKEAKDKTAEKVGEYKDYTVDKAVEARDYTAEKAIEAKDKTAEKTGE-YKDYTVEK 249

Query: 376 KEEAQRESMQTQAE-KESMYEKTKES---FEGAREEVKERGRGVGYEKRGEEG--RAGD 407
             E +  ++    E K+S  E  K +     G  EE K  G+ V  +   +E   +AG+
Sbjct: 250 ATEGKDVTVSKLGELKDSAVETAKRAMGFLSGKTEEAK--GKAVETKDTAKENMEKAGE 305

BLAST of MC02g0571 vs. ExPASy Swiss-Prot
Match: Q03968 (Late embryogenesis abundant protein, group 3 OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 4.1e-04
Identity = 55/183 (30.05%), Postives = 83/183 (45.36%), Query Frame = 0

Query: 152 MASAQNQPSLEEISNYRAIAQQKSNEAIMAARERYDKANQESLNK-----EAAKQRGTEG 211
           MAS QNQ S       +A  ++K+ + + A +++  +  + +  K     EA KQ+  E 
Sbjct: 1   MASNQNQASY-HAGETKARNEEKTGQVMGATKDKAGQTTEATKQKAGETTEATKQKAAET 60

Query: 212 KETTKEGGQTGLETVKETLTSA-------AQTAKEYTAPVAEKAKDYTVSAGETTAHYLG 271
            E  K+      E  K+    A       AQ AKE T   A+ AK+      + TA  LG
Sbjct: 61  TEAAKQKASETAEATKQKAAEAKDKTAQTAQAAKEKTYETAQSAKERAAQGKDQTASTLG 120

Query: 272 EKAVAAKDATIESGKVAAEYAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKG 323
           EK  AAK    E+ + A + A +A    K KA+     TA YT ++AV G      +++ 
Sbjct: 121 EKTEAAKQKAAETTEAARQKAAEATEAAKQKAS----ETAQYTKESAVTGKDKTGSVLQQ 178

BLAST of MC02g0571 vs. NCBI nr
Match: XP_022970889.1 (seed biotin-containing protein SBP65-like [Cucurbita maxima])

HSP 1 Score: 590 bits (1521), Expect = 1.98e-206
Identity = 361/496 (72.78%), Postives = 409/496 (82.46%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MATEKLSRRDDTT E+E +VE+ERVPQLTSHFEAIA+ GK TDP+N KA+  ++T   QK
Sbjct: 1   MATEKLSRRDDTTKEKEIQVERERVPQLTSHFEAIAVHGKATDPSNEKATTPEETKARQK 60

Query: 61  S----AGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRET 120
           S     GK+ +G G +  T  G++EERQ V  GG K E++H+LAAQFESLADKVRD+RET
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGV--GGEKPEDSHELAAQFESLADKVRDKRET 120

Query: 121 DTENERERQARADKQR------QETG----EQQRVSQQTKGKEGEMASAQNQPSLEEISN 180
           DTENERERQARADK+R      ++ G    E+QR  +Q K +EGE+ S QNQPSLEEISN
Sbjct: 121 DTENERERQARADKERIAAREREQKGSRAEEKQRALEQAKEREGEIGSRQNQPSLEEISN 180

Query: 181 YRAIAQQKSNEAIMAARERYDKANQESLNK------EAAKQRGTE-GKETTKEGGQ-TGL 240
           YRAIAQQKSNEAIMAA+ERYDKAN++SL +        A  RGTE  ++T +EG Q +GL
Sbjct: 181 YRAIAQQKSNEAIMAAKERYDKANKKSLEQGVEAGTREAIPRGTEEAEDTVEEGNQYSGL 240

Query: 241 ETVKETLTSAAQTAKEYTAPVAEKAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAE 300
           ETVKETLTSAA+TAK+YTAP+AEKAKDYTVSAG TTAHYLGEKAV AKD  IESGKVAAE
Sbjct: 241 ETVKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAE 300

Query: 301 YAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV 360
           YAGKAA DLKDK  VAGWSTAHY+CDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV
Sbjct: 301 YAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV 360

Query: 361 AESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYEKR 420
           AESTGE++KDYTARKKEEAQRESM  Q EKESMYEKTKE+FE   EEVKERG   G E R
Sbjct: 361 AESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEEGIEEVKERGGERGEENR 420

Query: 421 GEEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARGQGY 474
            EE R  DGG+LGAIGETIYEIAQTTKD+VIGPGDETGKQSTLGF+LG  EE+E +GQ +
Sbjct: 421 VEEDR-NDGGILGAIGETIYEIAQTTKDIVIGPGDETGKQSTLGFELGQ-EEDEEKGQEH 480

BLAST of MC02g0571 vs. NCBI nr
Match: XP_023534218.1 (seed biotin-containing protein SBP65-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588 bits (1517), Expect = 8.02e-206
Identity = 362/496 (72.98%), Postives = 412/496 (83.06%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MATEKLSRRDDTT E+E +VE+ERVPQL SHFEAIA+ GK TDP+N KA+  ++T   Q+
Sbjct: 1   MATEKLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKATDPSNEKATTPEETKARQR 60

Query: 61  S----AGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRET 120
           S     GK+ +G G +  T  G++EERQ V  GG K E++H+LAAQFESLADKVRD+RET
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGV--GGEKPEDSHELAAQFESLADKVRDKRET 120

Query: 121 DTENERERQARADKQR------QETG----EQQRVSQQTKGKEGEMASAQNQPSLEEISN 180
           +TENERERQARADK+R      ++ G    E+QRVS+Q K KEGE+ S QNQPSLEEISN
Sbjct: 121 NTENERERQARADKERIAAREREQKGSRAEEKQRVSEQAKEKEGEIGSRQNQPSLEEISN 180

Query: 181 YRAIAQQKSNEAIMAARERYDKANQESLNK--EA----AKQRGTE-GKETTKEGGQ-TGL 240
           YRAIAQQKSNEAIMAA+ERYDKAN++SL +  EA    A  RGTE  ++T +EG Q TGL
Sbjct: 181 YRAIAQQKSNEAIMAAKERYDKANKKSLQQGVEAGTGEAIPRGTEEAEDTVEEGNQYTGL 240

Query: 241 ETVKETLTSAAQTAKEYTAPVAEKAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAE 300
           ETVKETLTSAA+TAK+YTAP+AEKAKDYTVSAG TTAHYLGEKAV AKD  IESGKVAA 
Sbjct: 241 ETVKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAG 300

Query: 301 YAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV 360
           YAGKAA DLKDK  VAGWSTAHY+CDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV
Sbjct: 301 YAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV 360

Query: 361 AESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYEKR 420
           AESTGE++KDYTARKKEEAQRESM  Q EK+SMYEKTKE+FEG  EEVKERG   G E R
Sbjct: 361 AESTGETVKDYTARKKEEAQRESMYKQTEKDSMYEKTKETFEGGIEEVKERGGERGGENR 420

Query: 421 GEEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARGQGY 474
            EE R+ DGG+LGAIGETIYEIAQTTK++VIGPGDETGKQSTLGF+LG  EE E +GQ +
Sbjct: 421 VEEDRS-DGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQ-EEGEEKGQEH 480

BLAST of MC02g0571 vs. NCBI nr
Match: KAG7034893.1 (Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 586 bits (1511), Expect = 6.31e-205
Identity = 360/495 (72.73%), Postives = 411/495 (83.03%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MATE+LSRRDDTT E+E +VE+ERVPQL SHFEAIA+ GK TDP+N KA+  ++T   Q+
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  S----AGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRET 120
           S     GK+ +G G +  T  G++EERQ V  GG K E++H+LAAQFESLADKVRD+RET
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGV--GGEKPEDSHELAAQFESLADKVRDKRET 120

Query: 121 DTENERERQARADKQR------QETG---EQQRVSQQTKGKEGEMASAQNQPSLEEISNY 180
           +TENERERQARADK+R      ++ G   E++R S+Q K KEGE+ S Q+QP+LEEISNY
Sbjct: 121 NTENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNY 180

Query: 181 RAIAQQKSNEAIMAARERYDKANQESLNK--EA----AKQRGTE-GKETTKEGGQ-TGLE 240
           RAIAQQKSNEAIMAA+ERYDKAN++SL K  EA    A  RGTE  ++T +EG Q TGLE
Sbjct: 181 RAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLE 240

Query: 241 TVKETLTSAAQTAKEYTAPVAEKAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAEY 300
           TVKETLTSAA+TAK+YTAP+AEKAKDYTVSAG TTAHYLGEKAV AKD  IESGKVAAEY
Sbjct: 241 TVKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEY 300

Query: 301 AGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA 360
           AGKAA DLKDK  VAGWSTAHY+CDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA
Sbjct: 301 AGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA 360

Query: 361 ESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYEKRG 420
           ESTGE++KDYTARKKEEAQRESM  Q EKESMYEKTKE+FEG  EEVKERG   G E R 
Sbjct: 361 ESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRV 420

Query: 421 EEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARGQGYE 474
           EE R  DGG+LGAIGETIYEIAQTTK++VIGPGDETGKQSTLGF+LG  EE E +GQ +E
Sbjct: 421 EEDRK-DGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQ-EEGEEKGQEHE 480

BLAST of MC02g0571 vs. NCBI nr
Match: KAG6604765.1 (Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 585 bits (1507), Expect = 2.55e-204
Identity = 359/495 (72.53%), Postives = 410/495 (82.83%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MATE+LSRRDDTT E+E +VE+ERVPQL SHFEAIA+ GK TDP+N KA+  ++T   Q+
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  S----AGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRET 120
           S     GK+ +G G +  T  G++EERQ V  GG K E++H+LAAQFESLADKVRD+RET
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGV--GGEKPEDSHELAAQFESLADKVRDKRET 120

Query: 121 DTENERERQARADKQR------QETG---EQQRVSQQTKGKEGEMASAQNQPSLEEISNY 180
           +TENERERQARADK+R      ++ G   E++R S+Q K KEGE+ S Q+QP+LEEISNY
Sbjct: 121 NTENERERQARADKERIAAREREQKGREEEKRRASEQAKEKEGEIGSRQDQPTLEEISNY 180

Query: 181 RAIAQQKSNEAIMAARERYDKANQESLNK--EA----AKQRGTE-GKETTKEGGQ-TGLE 240
           RAIAQQKSNEAIMAA+ERYDKAN++SL K  EA    A  RGTE  ++T +EG Q TGLE
Sbjct: 181 RAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLE 240

Query: 241 TVKETLTSAAQTAKEYTAPVAEKAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAEY 300
           T KETLTSAA+TAK+YTAP+AEKAKDYTVSAG TTAHYLGEKAV AKD  IESGKVAAEY
Sbjct: 241 TAKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEY 300

Query: 301 AGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA 360
           AGKAA DLKDK  VAGWSTAHY+CDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA
Sbjct: 301 AGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA 360

Query: 361 ESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYEKRG 420
           ESTGE++KDYTARKKEEAQRESM  Q EKESMYEKTKE+FEG  EEVKERG   G E R 
Sbjct: 361 ESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRV 420

Query: 421 EEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARGQGYE 474
           EE R  DGG+LGAIGETIYEIAQTTK++VIGPGDETGKQSTLGF+LG  EE E +GQ +E
Sbjct: 421 EEDRK-DGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQ-EEGEEKGQEHE 480

BLAST of MC02g0571 vs. NCBI nr
Match: XP_022947224.1 (seed biotin-containing protein SBP65-like [Cucurbita moschata])

HSP 1 Score: 583 bits (1504), Expect = 7.29e-204
Identity = 359/495 (72.53%), Postives = 410/495 (82.83%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MATE+LSRRDDTT E+E +VE+ERVPQL SHFEAIA+ GK TDP+N KA+  ++T   Q+
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  S----AGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRET 120
           S     GK+ +G G +  T  G++EERQ V  GG K E++H+LAAQFESLADKVRD+RET
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGV--GGEKPEDSHELAAQFESLADKVRDKRET 120

Query: 121 DTENERERQARADKQR------QETG---EQQRVSQQTKGKEGEMASAQNQPSLEEISNY 180
           +TENERERQARADK+R      ++ G   E++R S+Q K KEGE+ S Q+QP+LEEISNY
Sbjct: 121 NTENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNY 180

Query: 181 RAIAQQKSNEAIMAARERYDKANQESLNK--EA----AKQRGTE-GKETTKEGGQ-TGLE 240
           RAIAQQKSNEAIMAA+ERYDKAN++SL K  EA    A  RGTE  ++T +EG Q TGLE
Sbjct: 181 RAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLE 240

Query: 241 TVKETLTSAAQTAKEYTAPVAEKAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAEY 300
           TVKETLTSAA+TAK+YTAP+AEKAKDYTVSAG TTAHYLGEKAV AKD  IESGKVAAEY
Sbjct: 241 TVKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEY 300

Query: 301 AGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA 360
           AGKAA DLKDK  VAGWSTAHY+CDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA
Sbjct: 301 AGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA 360

Query: 361 ESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYEKRG 420
           ESTGE++KDYTARKKEEAQRESM  Q EKESMYEKTKE+FEG  EEVKERG   G E R 
Sbjct: 361 ESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRV 420

Query: 421 EEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARGQGYE 474
           EE R  DGG+LGAIGETIYEIAQTTK++VIGPGDETGKQSTLGF+LG  EE E +GQ + 
Sbjct: 421 EEDRK-DGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQ-EEGEEKGQEHV 480

BLAST of MC02g0571 vs. ExPASy TrEMBL
Match: A0A6J1I6Z1 (seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC111469726 PE=4 SV=1)

HSP 1 Score: 590 bits (1521), Expect = 9.58e-207
Identity = 361/496 (72.78%), Postives = 409/496 (82.46%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MATEKLSRRDDTT E+E +VE+ERVPQLTSHFEAIA+ GK TDP+N KA+  ++T   QK
Sbjct: 1   MATEKLSRRDDTTKEKEIQVERERVPQLTSHFEAIAVHGKATDPSNEKATTPEETKARQK 60

Query: 61  S----AGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRET 120
           S     GK+ +G G +  T  G++EERQ V  GG K E++H+LAAQFESLADKVRD+RET
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGV--GGEKPEDSHELAAQFESLADKVRDKRET 120

Query: 121 DTENERERQARADKQR------QETG----EQQRVSQQTKGKEGEMASAQNQPSLEEISN 180
           DTENERERQARADK+R      ++ G    E+QR  +Q K +EGE+ S QNQPSLEEISN
Sbjct: 121 DTENERERQARADKERIAAREREQKGSRAEEKQRALEQAKEREGEIGSRQNQPSLEEISN 180

Query: 181 YRAIAQQKSNEAIMAARERYDKANQESLNK------EAAKQRGTE-GKETTKEGGQ-TGL 240
           YRAIAQQKSNEAIMAA+ERYDKAN++SL +        A  RGTE  ++T +EG Q +GL
Sbjct: 181 YRAIAQQKSNEAIMAAKERYDKANKKSLEQGVEAGTREAIPRGTEEAEDTVEEGNQYSGL 240

Query: 241 ETVKETLTSAAQTAKEYTAPVAEKAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAE 300
           ETVKETLTSAA+TAK+YTAP+AEKAKDYTVSAG TTAHYLGEKAV AKD  IESGKVAAE
Sbjct: 241 ETVKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAE 300

Query: 301 YAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV 360
           YAGKAA DLKDK  VAGWSTAHY+CDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV
Sbjct: 301 YAGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNV 360

Query: 361 AESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYEKR 420
           AESTGE++KDYTARKKEEAQRESM  Q EKESMYEKTKE+FE   EEVKERG   G E R
Sbjct: 361 AESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEEGIEEVKERGGERGEENR 420

Query: 421 GEEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARGQGY 474
            EE R  DGG+LGAIGETIYEIAQTTKD+VIGPGDETGKQSTLGF+LG  EE+E +GQ +
Sbjct: 421 VEEDR-NDGGILGAIGETIYEIAQTTKDIVIGPGDETGKQSTLGFELGQ-EEDEEKGQEH 480

BLAST of MC02g0571 vs. ExPASy TrEMBL
Match: A0A6J1G664 (seed biotin-containing protein SBP65-like OS=Cucurbita moschata OX=3662 GN=LOC111451152 PE=4 SV=1)

HSP 1 Score: 583 bits (1504), Expect = 3.53e-204
Identity = 359/495 (72.53%), Postives = 410/495 (82.83%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MATE+LSRRDDTT E+E +VE+ERVPQL SHFEAIA+ GK TDP+N KA+  ++T   Q+
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  S----AGKDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRET 120
           S     GK+ +G G +  T  G++EERQ V  GG K E++H+LAAQFESLADKVRD+RET
Sbjct: 61  SDASGVGKETYGTGIARGTVQGREEERQGV--GGEKPEDSHELAAQFESLADKVRDKRET 120

Query: 121 DTENERERQARADKQR------QETG---EQQRVSQQTKGKEGEMASAQNQPSLEEISNY 180
           +TENERERQARADK+R      ++ G   E++R S+Q K KEGE+ S Q+QP+LEEISNY
Sbjct: 121 NTENERERQARADKERIAAREREQKGRAEEKRRASEQAKEKEGEIGSRQDQPTLEEISNY 180

Query: 181 RAIAQQKSNEAIMAARERYDKANQESLNK--EA----AKQRGTE-GKETTKEGGQ-TGLE 240
           RAIAQQKSNEAIMAA+ERYDKAN++SL K  EA    A  RGTE  ++T +EG Q TGLE
Sbjct: 181 RAIAQQKSNEAIMAAKERYDKANKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLE 240

Query: 241 TVKETLTSAAQTAKEYTAPVAEKAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAEY 300
           TVKETLTSAA+TAK+YTAP+AEKAKDYTVSAG TTAHYLGEKAV AKD  IESGKVAAEY
Sbjct: 241 TVKETLTSAAKTAKDYTAPIAEKAKDYTVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEY 300

Query: 301 AGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA 360
           AGKAA DLKDK  VAGWSTAHY+CDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA
Sbjct: 301 AGKAAEDLKDKTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVA 360

Query: 361 ESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYEKRG 420
           ESTGE++KDYTARKKEEAQRESM  Q EKESMYEKTKE+FEG  EEVKERG   G E R 
Sbjct: 361 ESTGETVKDYTARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERGGERGEENRV 420

Query: 421 EEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARGQGYE 474
           EE R  DGG+LGAIGETIYEIAQTTK++VIGPGDETGKQSTLGF+LG  EE E +GQ + 
Sbjct: 421 EEDRK-DGGILGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQ-EEGEEKGQEHV 480

BLAST of MC02g0571 vs. ExPASy TrEMBL
Match: A0A6J1FUV3 (seed biotin-containing protein SBP65-like OS=Cucurbita moschata OX=3662 GN=LOC111448646 PE=4 SV=1)

HSP 1 Score: 528 bits (1359), Expect = 3.44e-182
Identity = 329/499 (65.93%), Postives = 382/499 (76.55%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MA E+L+RRD+TT ERE +VEKERVPQLTSHFEAIA+Q         KA+  +KT +   
Sbjct: 1   MAAEQLNRRDNTTKEREIQVEKERVPQLTSHFEAIAVQ--------CKAATPEKTHELAS 60

Query: 61  SAGKDAHGVGKSSDTSIGKDEERQRVSG--GGGKSEETHKLAAQFESLADKVRDRRETDT 120
             GK+A G   +     G++  R    G  GGGK ++TH+LAAQF+SLA+KVRD+RE DT
Sbjct: 61  DGGKEAQGTSIAKGALTGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKREPDT 120

Query: 121 ENERERQARADKQRQET----GEQQRVSQQTKGKEGEMASAQNQPSLEEISNYRAIAQQK 180
           ENERER+ARADK+R++     GE+QR+S+Q KGKEGE+ S Q QPSLEEISN RA AQ+K
Sbjct: 121 ENERERRARADKEREQRRSRIGEEQRLSEQKKGKEGEIGSRQYQPSLEEISNVRATAQEK 180

Query: 181 SNEAIMAARERYDKANQESLNK------EAAKQRGTEGKETTKE--GGQTGLETVKETLT 240
           SN+   AA+ERYD+A +ESLN+      E   QRGTEG E T E  G QTGL  VKETLT
Sbjct: 181 SNKVTRAAKERYDEAKKESLNQGVGARTEEWDQRGTEGAEDTVEERGQQTGLGAVKETLT 240

Query: 241 SAAQTAKEYTAPVAEKAKDYTVS-----------AGETTAHYLGEKAVAAKDATIESGKV 300
           SAA+TAK+YT P+AEKAKDYTV             G+TTAHYLGEKAVAAKD  I  GKV
Sbjct: 241 SAAKTAKDYTVPIAEKAKDYTVQKAVEAKDITVCVGQTTAHYLGEKAVAAKDVAIGGGKV 300

Query: 301 AAEYAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAA 360
           AAEYAGKAA DLKDKA VAGWSTAHY+CDTAVEGTKAAARLVKGAAE AGAIAA+PLSAA
Sbjct: 301 AAEYAGKAAEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAARPLSAA 360

Query: 361 KNVAESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGY 420
           KNVAESTGES+K+YTARKKEEAQRES   QAEKESMYEKTKE+FEGAREEVKERG G   
Sbjct: 361 KNVAESTGESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVN 420

Query: 421 EKRGEEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARG 474
           EK   EG+  DGG+LGAIGETIYEIAQTTK++VIG GDET K ST  F+ G  EE +A  
Sbjct: 421 EKNAAEGKDDDGGILGAIGETIYEIAQTTKEIVIGHGDETRKHSTFDFEHGE-EEGQAND 480

BLAST of MC02g0571 vs. ExPASy TrEMBL
Match: A0A6J1JER8 (seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC111483844 PE=4 SV=1)

HSP 1 Score: 527 bits (1358), Expect = 4.71e-182
Identity = 330/499 (66.13%), Postives = 385/499 (77.15%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MA E+LSRRD+TT ERE +VEKE+VPQLTSHFEAIA+Q         KA+  +KT +   
Sbjct: 1   MAAEQLSRRDNTTKEREIQVEKEKVPQLTSHFEAIAVQ--------CKAATPEKTHELAS 60

Query: 61  SAGKDAHGVGKSSDTSIGKDEERQRVSG--GGGKSEETHKLAAQFESLADKVRDRRETDT 120
             GK+A G   +     G++  R    G  GGGK ++TH+LAAQF+SLA+KVRD+RETDT
Sbjct: 61  DGGKEAQGTSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDKRETDT 120

Query: 121 ENERERQARADKQRQETG----EQQRVSQQTKGKEGEMASAQNQPSLEEISNYRAIAQQK 180
           ENER+R+ARADK+R++ G    E+QR+S+Q KGKEG++ S QNQPSLEEISN RA  Q+K
Sbjct: 121 ENERKRRARADKEREQKGSRIGEEQRLSEQKKGKEGDIGSRQNQPSLEEISNVRATEQEK 180

Query: 181 SNEAIMAARERYDKANQESLNK------EAAKQRGTEGKETTKE--GGQTGLETVKETLT 240
           SN+A MAA+ERYD+A +ESLN+      E + QRGTEG E T E  G QTGL  V+ETLT
Sbjct: 181 SNKATMAAKERYDEAKKESLNQGVGARTEESDQRGTEGAEDTVEEKGQQTGLGAVEETLT 240

Query: 241 SAAQTAKEYTAPVAEKAKDYTV-----------SAGETTAHYLGEKAVAAKDATIESGKV 300
           SAA+TAK+YT P+AEKAKDYTV           + G+TTAHYLGEKAVAAKD  I  GKV
Sbjct: 241 SAAKTAKDYTVPIAEKAKDYTVQKAVEAKDITVTVGQTTAHYLGEKAVAAKDVVIGGGKV 300

Query: 301 AAEYAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAA 360
           AAEYAGKA  DLKDKA VAGWSTAHY+CDTAVEGTKAAARLVKGAAE AGAIAAKPLSAA
Sbjct: 301 AAEYAGKATEDLKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAAKPLSAA 360

Query: 361 KNVAESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGY 420
           K VAESTGES+K+YTARKKEEAQRES   QAEKESMYEKTKE+FEGAREEVKERG G   
Sbjct: 361 KTVAESTGESVKEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGEGRVN 420

Query: 421 EKRGEEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEEARG 474
           EK   EGR  DGG+LGAIGETIYEIAQTTK++VIG GDET KQ+T  F+ G  EE EA  
Sbjct: 421 EKNAAEGRD-DGGILGAIGETIYEIAQTTKEIVIGHGDETRKQNTFDFEHGE-EEGEAND 480

BLAST of MC02g0571 vs. ExPASy TrEMBL
Match: A0A5A7ULM4 (Seed biotin-containing protein SBP65 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G002010 PE=4 SV=1)

HSP 1 Score: 502 bits (1293), Expect = 1.58e-172
Identity = 314/476 (65.97%), Postives = 374/476 (78.57%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTDPTNAKASILQKTDDTQK 60
           MA+E+LSRRD+TT ERE +VEKERVPQLTSHFEAIA QGK T P        +KT++ Q+
Sbjct: 1   MASEQLSRRDNTTNEREIQVEKERVPQLTSHFEAIAGQGKATIP--------EKTEEKQR 60

Query: 61  SAG----KDAHGVGKSSDTSIGKDEERQRVSGGGGKSEETHKLAAQFESLADKVRDRRET 120
           S      K+ HG+  +     G++EER+RV G GGK  ETH+LA+QFESLA KVRD RET
Sbjct: 61  SGASGGRKEVHGISIAKGELPGREEERKRVEGEGGKPNETHELASQFESLAQKVRDNRET 120

Query: 121 DTENERERQARADKQRQETGEQQRVSQQTKGKEGEMASAQNQPSLEEISNYRAIAQQKSN 180
           DTENER+RQ R ++QR     +QR S+Q K KEGE+ + QNQPSL+EISNYRAIAQ++SN
Sbjct: 121 DTENERQRQDRENEQR---VSKQRDSEQAKEKEGEIGTRQNQPSLDEISNYRAIAQERSN 180

Query: 181 EAIMAARERYDKANQESLNK------EAAKQRGTE-GKETTKE--GGQTGLETVKETLTS 240
           EAI AA+ERY+KAN+ESL++      E ++QR TE G+E T E  G +TGLETVKETLTS
Sbjct: 181 EAIRAAKERYEKANKESLHQGVEGKTEESEQRETEEGEENTVEVGGERTGLETVKETLTS 240

Query: 241 AAQTAKEYTAPVAEKAKDYTV-----------SAGETTAHYLGEKAVAAKDATIESGKVA 300
           AA+TAK+YT P+AEKAKDYTV           SAG+TTAHYLGEKAVAAKD  +ESGKVA
Sbjct: 241 AAKTAKDYTVPIAEKAKDYTVQKAIEAKDITVSAGQTTAHYLGEKAVAAKDVAVESGKVA 300

Query: 301 AEYAGKAAGDLKDKATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAK 360
           AEYAGKAA DLKDKA VAGWSTAHY+CDTA+EGTKAAARLVKGAAEYAGAIA KPLSAAK
Sbjct: 301 AEYAGKAAEDLKDKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAGKPLSAAK 360

Query: 361 NVAESTGESMKDYTARKKEEAQRESMQTQAEKESMYEKTKESFEGAREEVKERGRGVGYE 420
           NVA+STGES+K+YTARKKE A+RE+     EKE+MYEKTK +FEG +EE+K    G G E
Sbjct: 361 NVAQSTGESVKEYTARKKEVAEREATYKTGEKETMYEKTKGTFEGGKEELK----GRGNE 420

Query: 421 KRGEEGRAGDGGLLGAIGETIYEIAQTTKDLVIGPGDETGKQSTLGFDLGLGEEEE 452
           KR ++    DGG+LGAIGETIYEIAQTTK++VIG GDE+GKQS LGF++G  E+ E
Sbjct: 421 KRVDD----DGGILGAIGETIYEIAQTTKEIVIGDGDESGKQSKLGFEIGEEEQYE 457

BLAST of MC02g0571 vs. TAIR 10
Match: AT2G42560.1 (late embryogenesis abundant domain-containing protein / LEA domain-containing protein )

HSP 1 Score: 94.7 bits (234), Expect = 2.1e-19
Identity = 170/622 (27.33%), Postives = 236/622 (37.94%), Query Frame = 0

Query: 1   MATEKLSRRDDTTAEREGRVEKERVPQLTSHFEAIAIQGKPTD--------------PTN 60
           MA+E+ +RR++   ERE +VEK+RVP++TSHFE++A +GK +D                +
Sbjct: 1   MASEQ-ARRENKVTEREVQVEKDRVPKMTSHFESMAEKGKDSDTHRHQTEGGGTQFVSLS 60

Query: 61  AKASILQKTD----DTQKSAGKDAHGVGK---SSDTSIGK----DEERQRVS-------- 120
            K S +  +D    +T+    +  H VGK   SSD+  GK     EE ++ S        
Sbjct: 61  DKGSNMPVSDEGEGETKMKRTQMPHSVGKFVTSSDSGTGKKKDEKEEHEKASLEDIHGYR 120

Query: 121 ---------------------------------------------------------GGG 180
                                                                    GGG
Sbjct: 121 ANAQQKSMDSIKAAEERYNKAKESLSHSGQEARGGRGEEMVGKGRDSGVRVSHVGAVGGG 180

Query: 181 GKSEETHKLAAQFESLADKVRDRRETDTENERERQARADK----QRQETGEQQRVSQQTK 240
           G  EE       F     +  +      E  RER+ +        R          QQ K
Sbjct: 181 GGGEEKESGVHGFHGEKARHAELLAAGGEEMREREGKESAGGVGGRSVKDTVAEKGQQAK 240

Query: 241 GKEGEMASAQNQPSLEEISNYRAIAQQKSNEAIMAARERYDKANQESLNKEAAKQRGTEG 300
              GE A      + E+       A +K  EA     E+  +A      K+ A Q+  E 
Sbjct: 241 ESVGEGAQKAGSATSEKAQRASEYATEKGKEAGNMTAEQAARA------KDYALQKAVEA 300

Query: 301 KETTKEGGQTGLETVKETLTSAAQ---TAKEYTAPVAEKAKDYTVSAGETTAHYL----- 360
           KET  E  Q   E +KET ++AA+    AK+YT   A +AKD      +  + Y+     
Sbjct: 301 KETAAEKAQRASEYMKETGSTAAEQAARAKDYTLQKAVEAKDVAAEKAQRASEYMTETGK 360

Query: 361 ----------------------------GEKAVAAKDATIESGKVAAEYAGKAAGDLKDK 420
                                       GE A   K+ T+E GK AA YAG AA    +K
Sbjct: 361 QAGNVAAQKGQEAASMTAKAKDYTVQKAGEAAGYIKETTVEGGKGAAHYAGVAA----EK 420

Query: 421 ATVAGWSTAHYTCDTAVEGTKAAARLVKGAAEYAG----AIAAKPLSAAKNVAESTGESM 431
           A   GW+ AH+T +  V+GTKA A  V+GA  YAG     + +K +   K  A    +++
Sbjct: 421 AAAVGWTAAHFTTEKVVQGTKAVAGTVEGAVGYAGHKAVEVGSKAVDLTKEKAAVAADTV 480

BLAST of MC02g0571 vs. TAIR 10
Match: AT2G36640.1 (embryonic cell protein 63 )

HSP 1 Score: 51.2 bits (121), Expect = 2.6e-06
Identity = 78/239 (32.64%), Postives = 105/239 (43.93%), Query Frame = 0

Query: 196 KEAAKQRGTEGKETTKEGGQTGLE--------TVKETLTSAAQTA------KEYTAPVAE 255
           ++A   +  E  E+TKEG Q   E        TV++   +A  TA      K+YT   A+
Sbjct: 70  RDAVVGKTHEAAESTKEGAQIASEKAVGAKDATVEKAKETADYTAEKVGEYKDYTVDKAK 129

Query: 256 KAKDYTVSAGETTAHYLGEKAVAAKDATIESGKVAAEYAGKAAGDLKDKATVAGWSTAHY 315
           +AKD T    + TA+Y  +KAV AKD T E      +YA   A + KDK       TA+Y
Sbjct: 130 EAKDTTAEKAKETANYTADKAVEAKDKTAEKIGEYKDYAVDKAVEAKDKTAEKAKETANY 189

Query: 316 TCDTAVEGTKAAAR--------LVKGAAEYAGAIAAKPLSAAKNVAESTGESMKDYTARK 375
           T D A E     A          V  A E     A K + A    AE TGE  KDYT  K
Sbjct: 190 TADKAKEAKDKTAEKVGEYKDYTVDKAVEARDYTAEKAIEAKDKTAEKTGE-YKDYTVEK 249

Query: 376 KEEAQRESMQTQAE-KESMYEKTKES---FEGAREEVKERGRGVGYEKRGEEG--RAGD 407
             E +  ++    E K+S  E  K +     G  EE K  G+ V  +   +E   +AG+
Sbjct: 250 ATEGKDVTVSKLGELKDSAVETAKRAMGFLSGKTEEAK--GKAVETKDTAKENMEKAGE 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q398461.0e-4736.74Seed biotin-containing protein SBP65 OS=Glycine max OX=3847 GN=SBP65 PE=2 SV=1[more]
Q410601.1e-3031.23Seed biotin-containing protein SBP65 OS=Pisum sativum OX=3888 GN=SBP65 PE=1 SV=1[more]
Q9SKP03.7e-0532.64Late embryogenesis abundant protein ECP63 OS=Arabidopsis thaliana OX=3702 GN=ECP... [more]
Q039684.1e-0430.05Late embryogenesis abundant protein, group 3 OS=Triticum aestivum OX=4565 PE=2 S... [more]
Match NameE-valueIdentityDescription
XP_022970889.11.98e-20672.78seed biotin-containing protein SBP65-like [Cucurbita maxima][more]
XP_023534218.18.02e-20672.98seed biotin-containing protein SBP65-like [Cucurbita pepo subsp. pepo][more]
KAG7034893.16.31e-20572.73Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. arg... [more]
KAG6604765.12.55e-20472.53Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022947224.17.29e-20472.53seed biotin-containing protein SBP65-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1I6Z19.58e-20772.78seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1G6643.53e-20472.53seed biotin-containing protein SBP65-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1FUV33.44e-18265.93seed biotin-containing protein SBP65-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JER84.71e-18266.13seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A5A7ULM41.58e-17265.97Seed biotin-containing protein SBP65 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT2G42560.12.1e-1927.33late embryogenesis abundant domain-containing protein / LEA domain-containing pr... [more]
AT2G36640.12.6e-0632.64embryonic cell protein 63 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 354..374
NoneNo IPR availableCOILSCoilCoilcoord: 172..199
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 447..474
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 74..136
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 341..409
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 137..167
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 187..219
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 187..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 346..404
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 429..474
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..167
NoneNo IPR availablePANTHERPTHR47877FAMILY NOT NAMEDcoord: 1..462
NoneNo IPR availablePANTHERPTHR47877:SF3LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN / LEA DOMAIN-CONTAINING PROTEINcoord: 1..462

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0571.1MC02g0571.1mRNA