MC02g0533 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0533
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionseptum-promoting GTP-binding protein 1
LocationMC02: 4339902 .. 4342232 (-)
RNA-Seq ExpressionMC02g0533
SyntenyMC02g0533
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCACGTTGTAATGATAGGATGATTGATGGGGTGAGGTGAAGAGCATAAGATTTTGCCACGTGTAGTTTGTCATGTGAAGAGGTTTTTTCAAATGACTTTTGCCACTCGAATTCAATTTGGACATTTGGTTTTATTACATCATACCGGTAAACCGCCCCTTCCGTTAAAGTCGTCACATTGATAGCTAAATGTTGTTGTAACTGACAAAAAGAAGAATAAATCCCAATATCCAAAATAAAATAAAATTCATATAATAATTCATATTCTAAAAATACCCCCCCCCCNNNNNNNNNNNCCCCCCCCGCTTTTTCCTAGCTCTGGTATGAAGAGCACCAAGATCCGGGCTTCCCATTTTCATGGCCAGGAGACCAATCCACAGTCTCACTCCGGGCCATCCACAACATCCCAACTATGCCTCACCCTTCCCGGAAACAGCCTCCCCGGCCGATGCATCGCCCCGCAACGGCCCTCAAGCGCTGCACTCGGAAATTTTTCGACACGCTTCGAATGTGTTACTTGAAGAAGCCTAATACATATCGGATGGTACCTGAAGCCACTGCCTCGGCTTCGGGCAATGTGGCTGATGATGGCTTTGCGGCCGAGACGATGACACCCTTGTCCCACAATCATGGCCTGGACAGAGATTTGGTGTCCCTCAAGATCAGCCTATTGGGTGACAGCCAAATCGGAAAGACAAGTTTTCTGGTAAGATCAATTGTCTGTTGTTGGTTGGAGTTGAATGTTGGATTCTGGCTCTCTCTTTGGGCTTCATCTTTCAGTTGTTAATTTTTTGAAGGAAAAGTATGTTGGGAATGAAATGGAAGACGGAAGCAGAGCGCAGAAAGGATTGAACTTGATGGATAAAACACTGATGGTTAGAGGGGCACGTATTTCGTATAGCATTTGGGAAGTGGGAGGTAATTACCTAAAGGATTTTAAAATGTGGACGTGCAGTTTTATGATTGGCTGGAGGAAGGGACTAACGTGGATGTGGATGCAGGTGATAGAAAATCTCTGGATTACATTCCAACAGCTTGCAAGGACTCTGTAGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACCCTAAACAGGTGAGACTTGTTTCTCTGATCTAATATTACTTTGGCTTTCAGATTCTAAACCAAGGTTTTTCGGTGATTTGCAGCATTTTAAGGTGGTATAGACAAGCACTGAAATGGAATCAGGTAGTTTAGAACTCGTTTGATAACGTTATGGAAATGTTAGTTTTGAATTTTCTTTTCCTTGGCTCATATCTGGGATTGCAACCAAAGTTTCTGAAAGCAAAAGTTCGGCCTTTTCCCTCACTTTTATGAAGGTAGAAAACTCTGTTTGTTTTGCAGACAGCAATTCCAATCTTGATAGGAACAAAGTTCGACGATTTTATCCAACTCCCCATAGAACTGCAATGGGCAATTGCAAGCGAGGTAAAAATCGAAATCTTGGGTTCCAAGATCCTGCAATACTATTCCATAATTTTTTAGAGATTCTCACCAAATGTATCAGAGTACATATAAATCCTTCAAGTTAGGTAAGCCAGAGAATAAAGAATGTCTCAGAATTTTCCTTGTTTTGACAGGCAAGAACATACGCGAAGGCACTGAATGCGCCGTTGTTCTTCTCTAGTGCAGCTTACAACATCAATGTTAATAAGATCTTCAAATTCATCACAGCAAAGCTCTTTGACCTACCATGGACGGTCGAGCGCAACTTGACAGTTGGAGAGCCTATAATTGATTTCTAGAGTTGTTCTTCCACCCTACATATGCAATCTGGTCCTAAAGAAACTCTGAGAATCATCCTGCACAAGGATGAGTGTCCATATTGCTTTGACAACTATGTCAGATGTAGCGGAGAAAGGCAGAGGCGCGGAGCCGCGTCTCCGTCTCCGTAGTTGAGATGCATCTGCACTGCTCAAAAGTTGTCGAAAGGCAAGTAGAGACAATTGAGTAATGACCACACTTCTCTAAGTGTACCAGATCAAGCATGTCACTGCAAATGCAGCTTGCATCCTTTCAGAAAGTTTTAGCTGTAACTCCATGTATATCTGTAGTCACGCTCAATATCTTCTCTTTGATGAGAAAATTGAAGTGTCTATCAAATTAAACATCCTTCCTGATCGATGTGATAAAATAAAGTTATGTTGTTTATCTTTAGACATGCAGATGCGGCTATGGTAAAAAGTAAAAAACACTTATTCAATAACAGACCATCCTGCGGCTTACAAAGCAGTCTGGAATCAGGTCCTCCTCTTACAGGAAAACATTGTACAGTATCCAAATGCATATAAGATATAAGTTTGTTTAT

mRNA sequence

CCACGTTGTAATGATAGGATGATTGATGGGGTGAGGTGAAGAGCATAAGATTTTGCCACGTGTAGTTTGTCATGTGAAGAGGTTTTTTCAAATGACTTTTGCCACTCGAATTCAATTTGGACATTTGGTTTTATTACATCATACCGGTAAACCGCCCCTTCCGTTAAAGTCGTCACATTGATAGCTAAATGTTGTTGTAACTGACAAAAAGAAGAATAAATCCCAATATCCAAAATAAAATAAAATTCATATAATAATTCATATTCTAAAAATACCCCCCCCCCNNNNNNNNNNNCCCCCCCCGCTTTTTCCTAGCTCTGGTATGAAGAGCACCAAGATCCGGGCTTCCCATTTTCATGGCCAGGAGACCAATCCACAGTCTCACTCCGGGCCATCCACAACATCCCAACTATGCCTCACCCTTCCCGGAAACAGCCTCCCCGGCCGATGCATCGCCCCGCAACGGCCCTCAAGCGCTGCACTCGGAAATTTTTCGACACGCTTCGAATGTGTTACTTGAAGAAGCCTAATACATATCGGATGGTACCTGAAGCCACTGCCTCGGCTTCGGGCAATGTGGCTGATGATGGCTTTGCGGCCGAGACGATGACACCCTTGTCCCACAATCATGGCCTGGACAGAGATTTGGTGTCCCTCAAGATCAGCCTATTGGGTGACAGCCAAATCGGAAAGACAAGTTTTCTGGAAAAGTATGTTGGGAATGAAATGGAAGACGGAAGCAGAGCGCAGAAAGGATTGAACTTGATGGATAAAACACTGATGGTTAGAGGGGCACGTATTTCGTATAGCATTTGGGAAGTGGGAGGTGATAGAAAATCTCTGGATTACATTCCAACAGCTTGCAAGGACTCTGTAGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACCCTAAACAGCATTTTAAGGTGGTATAGACAAGCACTGAAATGGAATCAGACAGCAATTCCAATCTTGATAGGAACAAAGTTCGACGATTTTATCCAACTCCCCATAGAACTGCAATGGGCAATTGCAAGCGAGGCAAGAACATACGCGAAGGCACTGAATGCGCCGTTGTTCTTCTCTAGTGCAGCTTACAACATCAATGTTAATAAGATCTTCAAATTCATCACAGCAAAGCTCTTTGACCTACCATGGACGGTCGAGCGCAACTTGACAGTTGGAGAGCCTATAATTGATTTCTAGAGTTGTTCTTCCACCCTACATATGCAATCTGGTCCTAAAGAAACTCTGAGAATCATCCTGCACAAGGATGAGTGTCCATATTGCTTTGACAACTATGTCAGATGTAGCGGAGAAAGGCAGAGGCGCGGAGCCGCGTCTCCGTCTCCGTAGTTGAGATGCATCTGCACTGCTCAAAAGTTGTCGAAAGGCAAGTAGAGACAATTGAGTAATGACCACACTTCTCTAAGTGTACCAGATCAAGCATGTCACTGCAAATGCAGCTTGCATCCTTTCAGAAAGTTTTAGCTGTAACTCCATGTATATCTGTAGTCACGCTCAATATCTTCTCTTTGATGAGAAAATTGAAGTGTCTATCAAATTAAACATCCTTCCTGATCGATGTGATAAAATAAAGTTATGTTGTTTATCTTTAGACATGCAGATGCGGCTATGGTAAAAAGTAAAAAACACTTATTCAATAACAGACCATCCTGCGGCTTACAAAGCAGTCTGGAATCAGGTCCTCCTCTTACAGGAAAACATTGTACAGTATCCAAATGCATATAAGATATAAGTTTGTTTAT

Coding sequence (CDS)

ATGCCTCACCCTTCCCGGAAACAGCCTCCCCGGCCGATGCATCGCCCCGCAACGGCCCTCAAGCGCTGCACTCGGAAATTTTTCGACACGCTTCGAATGTGTTACTTGAAGAAGCCTAATACATATCGGATGGTACCTGAAGCCACTGCCTCGGCTTCGGGCAATGTGGCTGATGATGGCTTTGCGGCCGAGACGATGACACCCTTGTCCCACAATCATGGCCTGGACAGAGATTTGGTGTCCCTCAAGATCAGCCTATTGGGTGACAGCCAAATCGGAAAGACAAGTTTTCTGGAAAAGTATGTTGGGAATGAAATGGAAGACGGAAGCAGAGCGCAGAAAGGATTGAACTTGATGGATAAAACACTGATGGTTAGAGGGGCACGTATTTCGTATAGCATTTGGGAAGTGGGAGGTGATAGAAAATCTCTGGATTACATTCCAACAGCTTGCAAGGACTCTGTAGCAATTCTTTTTATGTTTGATCTAACAAGTAGGCGTACCCTAAACAGCATTTTAAGGTGGTATAGACAAGCACTGAAATGGAATCAGACAGCAATTCCAATCTTGATAGGAACAAAGTTCGACGATTTTATCCAACTCCCCATAGAACTGCAATGGGCAATTGCAAGCGAGGCAAGAACATACGCGAAGGCACTGAATGCGCCGTTGTTCTTCTCTAGTGCAGCTTACAACATCAATGTTAATAAGATCTTCAAATTCATCACAGCAAAGCTCTTTGACCTACCATGGACGGTCGAGCGCAACTTGACAGTTGGAGAGCCTATAATTGATTTCTAG

Protein sequence

MPHPSRKQPPRPMHRPATALKRCTRKFFDTLRMCYLKKPNTYRMVPEATASASGNVADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF
Homology
BLAST of MC02g0533 vs. ExPASy Swiss-Prot
Match: P87027 (Septum-promoting GTP-binding protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spg1 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.4e-49
Identity = 91/187 (48.66%), Postives = 131/187 (70.05%), Query Frame = 0

Query: 80  VSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGG 139
           V++K+ ++GDS IGKTS +  YV    ++ S    G+N M+KT+ +R   I++SIW++GG
Sbjct: 9   VTIKVGMIGDSSIGKTSLMVTYVQGSFDEESTQTLGVNFMEKTISIRNTEITFSIWDLGG 68

Query: 140 DRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFI 199
            R+ ++ +P  C D+VAILFMFDL+ + TLNSI  WYRQA  +N+TA+PILIGTK+D F+
Sbjct: 69  QREFVNMLPMVCNDAVAILFMFDLSRKSTLNSIKEWYRQARGFNKTAVPILIGTKYDHFM 128

Query: 200 QLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTV 259
             P E Q  I  +AR YAKA+ A L F S +++INV KIFK + AK+FDL  T+     V
Sbjct: 129 TFPREDQEEITKQARRYAKAMKASLVFCSTSHSINVQKIFKIVLAKVFDLKCTIPEIKNV 188

Query: 260 GEPIIDF 267
           G+PI+++
Sbjct: 189 GDPILEY 195

BLAST of MC02g0533 vs. ExPASy Swiss-Prot
Match: P38987 (Protein TEM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TEM1 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 9.2e-38
Identity = 78/188 (41.49%), Postives = 118/188 (62.77%), Query Frame = 0

Query: 77  RDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWE 136
           R+ V +++ L+GD+Q+GKTS + KYV N  +       G+N + + + +R   I +SI +
Sbjct: 16  RNQVEVQVGLVGDAQVGKTSLMVKYVQNIYDKEYTQTLGVNFLKRKVSIRSTDIIFSIMD 75

Query: 137 VGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFD 196
           +GG R+ ++ +P A   S  I+F+FDLT   TL+SI  WYRQA   N +AIPIL+GTK+D
Sbjct: 76  LGGQREFINMLPIATVGSSVIIFLFDLTRPETLSSIKEWYRQAYGLNDSAIPILVGTKYD 135

Query: 197 DFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERN 256
             I L  E Q  I+  +  YA+ +NAPL F S A +IN+ KIFK   AK+F+L  T+   
Sbjct: 136 LLIDLDPEYQEQISRTSMKYAQVMNAPLIFCSTAKSINIQKIFKIALAKIFNLTLTIPEI 195

Query: 257 LTVGEPII 265
             +G+P++
Sbjct: 196 NEIGDPLL 203

BLAST of MC02g0533 vs. ExPASy Swiss-Prot
Match: P07560 (Ras-related protein SEC4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC4 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.9e-14
Identity = 53/192 (27.60%), Postives = 94/192 (48.96%), Query Frame = 0

Query: 70  SHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGAR 129
           S  +G   D + +KI L+GDS +GK+  L ++V ++         G++   KT+ + G +
Sbjct: 10  SSGNGKSYDSI-MKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDFKIKTVDINGKK 69

Query: 130 ISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQALKW-NQTAIP 189
           +   +W+  G  +         + ++ I+ ++D+T  RT  +I +W++   +  N  A  
Sbjct: 70  VKLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTFTNIKQWFKTVNEHANDEAQL 129

Query: 190 ILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFD 249
           +L+G K D      +E +   A +    AK L  P   SSA  + NVN+IF F  AKL  
Sbjct: 130 LLVGNKSD------METRVVTADQGEALAKELGIPFIESSAKNDDNVNEIF-FTLAKL-- 189

Query: 250 LPWTVERNLTVG 261
           +   ++ N  VG
Sbjct: 190 IQEKIDSNKLVG 191

BLAST of MC02g0533 vs. ExPASy Swiss-Prot
Match: Q9JKM7 (Ras-related protein Rab-37 OS=Mus musculus OX=10090 GN=Rab37 PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 4.2e-14
Identity = 58/201 (28.86%), Postives = 95/201 (47.26%), Query Frame = 0

Query: 49  TASASGNVADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMED 108
           T +     A DG A E   P S N+ L     + K+ LLGDS +GKT FL ++       
Sbjct: 2   TGTPGAATAGDGEAPERSPPFSPNYDL-----TGKVMLLGDSGVGKTCFLIQFKDGAFLS 61

Query: 109 GS-RAQKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRR 168
           G+  A  G++  +K + V GAR+   IW+  G  +         +D+ A+L ++D+T++ 
Sbjct: 62  GTFIATVGIDSRNKVVTVDGARVKLQIWDTAGQERFRSVTHAYYRDAQALLLLYDITNQS 121

Query: 169 TLNSILRWYRQALKWNQTAIPI-LIGTKFDDFIQLPIELQWAIASE-ARTYAKALNAPLF 228
           + ++I  W  +  ++ Q  + I L+G K D      +  +  I SE   T A+    P  
Sbjct: 122 SFDNIRAWLTEIHEYAQRDVVIMLLGNKAD------VSSERVIRSEDGETLAREYGVPFM 181

Query: 229 FSSAAYNINVNKIFKFITAKL 247
            +SA   +NV   F  I  +L
Sbjct: 182 ETSAKTGMNVELAFLAIAKEL 191

BLAST of MC02g0533 vs. ExPASy Swiss-Prot
Match: Q9FIF9 (Ras-related protein RABA2d OS=Arabidopsis thaliana OX=3702 GN=RABA2D PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.6e-13
Identity = 49/182 (26.92%), Postives = 88/182 (48.35%), Query Frame = 0

Query: 69  LSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGA 128
           ++H    D D +  KI L+GDS +GKT+ L ++  NE    S++  G+    +TL V G 
Sbjct: 1   MAHRVEQDYDYL-FKIVLIGDSGVGKTNILSRFTRNEFCLESKSTIGVEFATRTLQVEGK 60

Query: 129 RISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIP 188
            +   IW+  G  +         + +V  L ++D+T R+T +++LRW R+      + I 
Sbjct: 61  TVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDITKRQTFDNVLRWLRELRDHADSNIV 120

Query: 189 ILI-GTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLF 248
           I++ G K D        L+     + +T A+        +SA    NV K F+ + A+++
Sbjct: 121 IMMAGNKAD-----LNHLRSVAEEDGQTLAETEGLSFLETSALEATNVEKAFQTVLAEIY 176

Query: 249 DL 250
            +
Sbjct: 181 HI 176

BLAST of MC02g0533 vs. NCBI nr
Match: XP_022140324.1 (septum-promoting GTP-binding protein 1 [Momordica charantia] >XP_022140325.1 septum-promoting GTP-binding protein 1 [Momordica charantia])

HSP 1 Score: 474 bits (1219), Expect = 4.84e-168
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0

Query: 33  MCYLKKPNTYRMVPEATASASGNVADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQI 92
           MCYLKKPNTYRMVPEATASASGNVADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQI
Sbjct: 1   MCYLKKPNTYRMVPEATASASGNVADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQI 60

Query: 93  GKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACK 152
           GKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACK
Sbjct: 61  GKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACK 120

Query: 153 DSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASE 212
           DSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASE
Sbjct: 121 DSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASE 180

Query: 213 ARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 266
           ARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 181 ARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 234

BLAST of MC02g0533 vs. NCBI nr
Match: KAG7034967.1 (spg1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421 bits (1083), Expect = 2.06e-146
Identity = 215/274 (78.47%), Postives = 232/274 (84.67%), Query Frame = 0

Query: 1   MPHPSRKQP-----PRPMHRPATALKRCTRKFFDTLRMCYLKKPNTYRMVPEATASASGN 60
           MP P RKQP      R MH PATALKR TR+F +++  CY KKP T R V +  A+AS +
Sbjct: 19  MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSKKPQTCRTVRDDAATASIS 78

Query: 61  ---VADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRA 120
              VAD GF AET TPL HNH  D+DLV LKISLLGDSQIGKTSFLEKYVGNEME+G   
Sbjct: 79  PRAVADGGFEAETRTPLCHNHSQDKDLVPLKISLLGDSQIGKTSFLEKYVGNEMEEGKSE 138

Query: 121 QKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSI 180
           Q+GLNLMDKTLMVRGARISYSIWEVGGD+KS D+IPTACKDSVAILFMFDLTSRRTL S+
Sbjct: 139 QRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLKSV 198

Query: 181 LRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYN 240
           LRWYRQA KWNQ AIP+LIGTKFDDFIQLPI+LQWAIASEAR YAKALNA LFFSSA YN
Sbjct: 199 LRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIASEARAYAKALNATLFFSSATYN 258

Query: 241 INVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 266
           INVNKIFKFITAKLFDLPWT+ERNLTVGEP+IDF
Sbjct: 259 INVNKIFKFITAKLFDLPWTLERNLTVGEPMIDF 292

BLAST of MC02g0533 vs. NCBI nr
Match: XP_023534173.1 (septum-promoting GTP-binding protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419 bits (1077), Expect = 8.87e-146
Identity = 214/274 (78.10%), Postives = 231/274 (84.31%), Query Frame = 0

Query: 1   MPHPSRKQP-----PRPMHRPATALKRCTRKFFDTLRMCYLKKPNTYRMVPEATASASGN 60
           MP P RKQP      R MH PATALKR TR+F +++  CY KKP T R V +  A+AS +
Sbjct: 1   MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSKKPQTCRTVRDDAATASIS 60

Query: 61  ---VADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRA 120
              VAD GF AET TPL HNH  D+DLV LKISLLGDSQIGKTSFLEKYVGNEME+    
Sbjct: 61  PRAVADGGFEAETRTPLCHNHSQDKDLVPLKISLLGDSQIGKTSFLEKYVGNEMEESKSE 120

Query: 121 QKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSI 180
           Q+GLNLMDKTLMVRGARISYSIWEVGGD+KS D+IPTACKDSVAILFMFDLTSRRTL S+
Sbjct: 121 QRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLKSV 180

Query: 181 LRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYN 240
           LRWYRQA KWNQ AIP+LIGTKFDDFIQLPI+LQWAIASEAR YAKALNA LFFSSA YN
Sbjct: 181 LRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIASEARAYAKALNATLFFSSATYN 240

Query: 241 INVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 266
           INVNKIFKFITAKLFDLPWT+ERNLTVGEP+IDF
Sbjct: 241 INVNKIFKFITAKLFDLPWTLERNLTVGEPMIDF 274

BLAST of MC02g0533 vs. NCBI nr
Match: XP_022971065.1 (septum-promoting GTP-binding protein 1-like [Cucurbita maxima])

HSP 1 Score: 419 bits (1078), Expect = 1.19e-145
Identity = 214/274 (78.10%), Postives = 231/274 (84.31%), Query Frame = 0

Query: 1   MPHPSRKQP-----PRPMHRPATALKRCTRKFFDTLRMCYLKKPNTYRMVPEATASASGN 60
           MP P RKQP      R MH PATALKR TR+F +++  CY KKP T R V +  A+AS +
Sbjct: 19  MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSKKPQTCRTVRDDAATASIS 78

Query: 61  ---VADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRA 120
              VAD GF AET TPL HNH  D+DLV LKISLLGDSQIGKT FLEKYVGNEME+G   
Sbjct: 79  PRAVADGGFEAETRTPLCHNHSQDKDLVPLKISLLGDSQIGKTCFLEKYVGNEMEEGKSE 138

Query: 121 QKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSI 180
           Q+GLNLMDKTLMVRGARISYSIWEVGGD+KS D+IPTACKDSVAILFMFDLTSRRTL S+
Sbjct: 139 QRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLKSV 198

Query: 181 LRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYN 240
           LRWYRQA KWNQ AIP+LIGTKFDDFIQLPI+LQWAIA EARTYAKALNA LFFSSA YN
Sbjct: 199 LRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIACEARTYAKALNATLFFSSATYN 258

Query: 241 INVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 266
           INVNKIFKFITAKLFDLPWT+ERNLTVGEP+IDF
Sbjct: 259 INVNKIFKFITAKLFDLPWTLERNLTVGEPMIDF 292

BLAST of MC02g0533 vs. NCBI nr
Match: XP_022947099.1 (septum-promoting GTP-binding protein 1-like [Cucurbita moschata])

HSP 1 Score: 418 bits (1074), Expect = 4.82e-145
Identity = 214/274 (78.10%), Postives = 231/274 (84.31%), Query Frame = 0

Query: 1   MPHPSRKQP-----PRPMHRPATALKRCTRKFFDTLRMCYLKKPNTYRMVPEATASASGN 60
           MP P RKQP      R MH PATALKR TR+F +++  CY KKP T R V +  A+AS +
Sbjct: 19  MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSKKPQTCRTVCDDAATASIS 78

Query: 61  ---VADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRA 120
              VAD G  AET TPL HNH  D+DLV LKISLLGDSQIGKTSFLEKYVGNEME+G   
Sbjct: 79  PRAVADGGSEAETRTPLCHNHSQDKDLVPLKISLLGDSQIGKTSFLEKYVGNEMEEGKSE 138

Query: 121 QKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSI 180
           Q+GLNLMDKTLMVRGARISYSIWEVGGD+KS D+IPTACKDSVAILFMFDLTSRRTL S+
Sbjct: 139 QRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLKSV 198

Query: 181 LRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYN 240
           LRWYRQA KWNQ AIP+LIGTKFDDFIQLPI+LQWAIASEAR YAKALNA LFFSSA YN
Sbjct: 199 LRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIASEARAYAKALNATLFFSSATYN 258

Query: 241 INVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 266
           INVNKIFKFITAKLFDLPWT+ERNLTVGEP+IDF
Sbjct: 259 INVNKIFKFITAKLFDLPWTLERNLTVGEPMIDF 292

BLAST of MC02g0533 vs. ExPASy TrEMBL
Match: A0A6J1CFF3 (septum-promoting GTP-binding protein 1 OS=Momordica charantia OX=3673 GN=LOC111011026 PE=4 SV=1)

HSP 1 Score: 474 bits (1219), Expect = 2.34e-168
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0

Query: 33  MCYLKKPNTYRMVPEATASASGNVADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQI 92
           MCYLKKPNTYRMVPEATASASGNVADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQI
Sbjct: 1   MCYLKKPNTYRMVPEATASASGNVADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQI 60

Query: 93  GKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACK 152
           GKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACK
Sbjct: 61  GKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACK 120

Query: 153 DSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASE 212
           DSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASE
Sbjct: 121 DSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASE 180

Query: 213 ARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 266
           ARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 181 ARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 234

BLAST of MC02g0533 vs. ExPASy TrEMBL
Match: A0A6J1I4P4 (septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469846 PE=4 SV=1)

HSP 1 Score: 419 bits (1078), Expect = 5.75e-146
Identity = 214/274 (78.10%), Postives = 231/274 (84.31%), Query Frame = 0

Query: 1   MPHPSRKQP-----PRPMHRPATALKRCTRKFFDTLRMCYLKKPNTYRMVPEATASASGN 60
           MP P RKQP      R MH PATALKR TR+F +++  CY KKP T R V +  A+AS +
Sbjct: 19  MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSKKPQTCRTVRDDAATASIS 78

Query: 61  ---VADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRA 120
              VAD GF AET TPL HNH  D+DLV LKISLLGDSQIGKT FLEKYVGNEME+G   
Sbjct: 79  PRAVADGGFEAETRTPLCHNHSQDKDLVPLKISLLGDSQIGKTCFLEKYVGNEMEEGKSE 138

Query: 121 QKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSI 180
           Q+GLNLMDKTLMVRGARISYSIWEVGGD+KS D+IPTACKDSVAILFMFDLTSRRTL S+
Sbjct: 139 QRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLKSV 198

Query: 181 LRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYN 240
           LRWYRQA KWNQ AIP+LIGTKFDDFIQLPI+LQWAIA EARTYAKALNA LFFSSA YN
Sbjct: 199 LRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIACEARTYAKALNATLFFSSATYN 258

Query: 241 INVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 266
           INVNKIFKFITAKLFDLPWT+ERNLTVGEP+IDF
Sbjct: 259 INVNKIFKFITAKLFDLPWTLERNLTVGEPMIDF 292

BLAST of MC02g0533 vs. ExPASy TrEMBL
Match: A0A6J1G5T0 (septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451069 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 2.33e-145
Identity = 214/274 (78.10%), Postives = 231/274 (84.31%), Query Frame = 0

Query: 1   MPHPSRKQP-----PRPMHRPATALKRCTRKFFDTLRMCYLKKPNTYRMVPEATASASGN 60
           MP P RKQP      R MH PATALKR TR+F +++  CY KKP T R V +  A+AS +
Sbjct: 19  MPQPCRKQPLNFGLKRQMHHPATALKRRTRQFLESILTCYSKKPQTCRTVCDDAATASIS 78

Query: 61  ---VADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRA 120
              VAD G  AET TPL HNH  D+DLV LKISLLGDSQIGKTSFLEKYVGNEME+G   
Sbjct: 79  PRAVADGGSEAETRTPLCHNHSQDKDLVPLKISLLGDSQIGKTSFLEKYVGNEMEEGKSE 138

Query: 121 QKGLNLMDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSI 180
           Q+GLNLMDKTLMVRGARISYSIWEVGGD+KS D+IPTACKDSVAILFMFDLTSRRTL S+
Sbjct: 139 QRGLNLMDKTLMVRGARISYSIWEVGGDKKSQDHIPTACKDSVAILFMFDLTSRRTLKSV 198

Query: 181 LRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYN 240
           LRWYRQA KWNQ AIP+LIGTKFDDFIQLPI+LQWAIASEAR YAKALNA LFFSSA YN
Sbjct: 199 LRWYRQAWKWNQAAIPVLIGTKFDDFIQLPIDLQWAIASEARAYAKALNATLFFSSATYN 258

Query: 241 INVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 266
           INVNKIFKFITAKLFDLPWT+ERNLTVGEP+IDF
Sbjct: 259 INVNKIFKFITAKLFDLPWTLERNLTVGEPMIDF 292

BLAST of MC02g0533 vs. ExPASy TrEMBL
Match: A0A6J1JFU6 (septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111484119 PE=4 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 6.72e-137
Identity = 203/268 (75.75%), Postives = 224/268 (83.58%), Query Frame = 0

Query: 1   MPHPSRKQPPRPMHRPATALKRCTRKFFDTLRMCYLK-KPNTYRMVPEATASASGNVADD 60
           MP P RKQP R   +  TALKR   +F D++  CY   KP  Y  VP    +AS  V D 
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILACYSSTKPKAYPAVPTEATAASTVVDDA 60

Query: 61  GFAAETMTP-LSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRAQKGLNL 120
           GFAAET+TP + H+HGLD+DLVSLKISLLGDSQIGKTSFL KYVGNE+++G   Q GLNL
Sbjct: 61  GFAAETLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 MDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQ 180
           MDKTLMVRGARISYSIWEV GD+KS DYIPTACKDSVAILFMFDLTSRRTL  I++WYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKI 240
           A K+NQ AIP+L+GTKFDDFIQLPI+LQWAIA+EARTYAKALNA LFFSS+ YNINVNKI
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARTYAKALNATLFFSSSTYNINVNKI 240

Query: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 266
           FKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 268

BLAST of MC02g0533 vs. ExPASy TrEMBL
Match: A0A6J1FYS5 (septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449038 PE=4 SV=1)

HSP 1 Score: 394 bits (1011), Expect = 3.87e-136
Identity = 201/268 (75.00%), Postives = 224/268 (83.58%), Query Frame = 0

Query: 1   MPHPSRKQPPRPMHRPATALKRCTRKFFDTLRMCYLK-KPNTYRMVPEATASASGNVADD 60
           MP P RKQP R   +  TALKR   +F D++  CY   KP  Y  VP+   +AS  V D 
Sbjct: 1   MPQPCRKQPLRLSVKRQTALKRHAGQFLDSILSCYSSTKPKAYPAVPDEATAASTVVEDA 60

Query: 61  GFAAETMTP-LSHNHGLDRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSRAQKGLNL 120
           GFAAE++TP + H+HGLD+DLVSLKISLLGDSQIGKTSFL KYVGNE+++G   Q GLNL
Sbjct: 61  GFAAESLTPSVCHDHGLDKDLVSLKISLLGDSQIGKTSFLVKYVGNELDEGRSEQTGLNL 120

Query: 121 MDKTLMVRGARISYSIWEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQ 180
           MDKTLMVRGARISYSIWEV GD+KS DYIPTACKDSVAILFMFDLTSRRTL  I++WYRQ
Sbjct: 121 MDKTLMVRGARISYSIWEVEGDKKSQDYIPTACKDSVAILFMFDLTSRRTLTGIVKWYRQ 180

Query: 181 ALKWNQTAIPILIGTKFDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKI 240
           A K+NQ AIP+L+GTKFDDFIQLPI+LQWAIA+EAR YAKALNA LFFSS+ YNINVNKI
Sbjct: 181 ARKYNQRAIPVLVGTKFDDFIQLPIDLQWAIANEARAYAKALNATLFFSSSTYNINVNKI 240

Query: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 266
           FKFITAKLFDLPWTVERNLTVGEPIIDF
Sbjct: 241 FKFITAKLFDLPWTVERNLTVGEPIIDF 268

BLAST of MC02g0533 vs. TAIR 10
Match: AT3G21700.3 (Ras-related small GTP-binding family protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.4e-81
Identity = 148/192 (77.08%), Postives = 166/192 (86.46%), Query Frame = 0

Query: 76  DRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSR-AQKGLNLMDKTLMVRGARISYSI 135
           D DLVSLKISLLGD +IGKTSFL KYVG E E   R  +KG+N  DKTL + GARISYSI
Sbjct: 101 DSDLVSLKISLLGDPEIGKTSFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSI 160

Query: 136 WEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTK 195
           WE+ G  +S D IP ACKDSVAILFMFDLTSR TLNS++ WY+QA K NQTAIP+++GTK
Sbjct: 161 WELEGAERSRDQIPVACKDSVAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTK 220

Query: 196 FDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVE 255
           FD+FIQLPI+LQW IAS+ARTYAKALNA LFFSSA+YNINVNKIFKF+TAKLFDLPWTVE
Sbjct: 221 FDEFIQLPIDLQWTIASQARTYAKALNATLFFSSASYNINVNKIFKFVTAKLFDLPWTVE 280

Query: 256 RNLTVGEPIIDF 267
           RNLT+GEPIIDF
Sbjct: 281 RNLTIGEPIIDF 292

BLAST of MC02g0533 vs. TAIR 10
Match: AT3G21700.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 295.8 bits (756), Expect = 3.4e-80
Identity = 148/192 (77.08%), Postives = 166/192 (86.46%), Query Frame = 0

Query: 76  DRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSR-AQKGLNLMDKTLMVRGARISYSI 135
           D DLVSLKISLLGD +IGKTSFL KYVG E E   R  +KG+N  DKTL + GARISYSI
Sbjct: 101 DSDLVSLKISLLGDPEIGKTSFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSI 160

Query: 136 WEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTK 195
           WE+  +R S D IP ACKDSVAILFMFDLTSR TLNS++ WY+QA K NQTAIP+++GTK
Sbjct: 161 WELEAER-SRDQIPVACKDSVAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTK 220

Query: 196 FDDFIQLPIELQWAIASEARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVE 255
           FD+FIQLPI+LQW IAS+ARTYAKALNA LFFSSA+YNINVNKIFKF+TAKLFDLPWTVE
Sbjct: 221 FDEFIQLPIDLQWTIASQARTYAKALNATLFFSSASYNINVNKIFKFVTAKLFDLPWTVE 280

Query: 256 RNLTVGEPIIDF 267
           RNLT+GEPIIDF
Sbjct: 281 RNLTIGEPIIDF 291

BLAST of MC02g0533 vs. TAIR 10
Match: AT5G54840.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 283.5 bits (724), Expect = 1.8e-76
Identity = 143/235 (60.85%), Postives = 174/235 (74.04%), Query Frame = 0

Query: 36  LKKPNTYRMVPEATASASGNV---ADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQI 95
           ++KP  YR +    +S +G++    DD     T++        D DLVSLKISLLGD Q 
Sbjct: 53  IEKPILYRRIVHKISSTAGDIFDGDDDTTTEPTVSGRRFGSSSDSDLVSLKISLLGDCQT 112

Query: 96  GKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRK-SLDYIPTAC 155
           GKT+F+ KYVG+E         GLNLMDKT  V+G  IS+SIW+VGGD K S D+IP AC
Sbjct: 113 GKTTFVVKYVGDE-NQSFLEMTGLNLMDKTFYVQGVTISFSIWDVGGDEKRSKDHIPIAC 172

Query: 156 KDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTKFDDFIQLPIELQWAIAS 215
           KD+VAILFMFDLTSR TLNS+  WY QA KWN+TAIPILIGTKFDDF++LP  LQW I +
Sbjct: 173 KDAVAILFMFDLTSRSTLNSVFGWYSQARKWNKTAIPILIGTKFDDFVRLPPNLQWTIVT 232

Query: 216 EARTYAKALNAPLFFSSAAYNINVNKIFKFITAKLFDLPWTVERNLTVGEPIIDF 267
           +AR YAK +NA LFFSSA +NINVNKIFKFI A+LF+LPW ++RNLT+GEPIID+
Sbjct: 233 QARAYAKVMNASLFFSSATHNINVNKIFKFILARLFNLPWKIDRNLTLGEPIIDY 286

BLAST of MC02g0533 vs. TAIR 10
Match: AT3G21700.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 193.4 bits (490), Expect = 2.4e-49
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0

Query: 76  DRDLVSLKISLLGDSQIGKTSFLEKYVGNEMEDGSR-AQKGLNLMDKTLMVRGARISYSI 135
           D DLVSLKISLLGD +IGKTSFL KYVG E E   R  +KG+N  DKTL + GARISYSI
Sbjct: 101 DSDLVSLKISLLGDPEIGKTSFLAKYVGEEKEVEMRELEKGINCTDKTLYMGGARISYSI 160

Query: 136 WEVGGDRKSLDYIPTACKDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAIPILIGTK 195
           WE+  +R S D IP ACKDSVAILFMFDLTSR TLNS++ WY+QA K NQTAIP+++GTK
Sbjct: 161 WELEAER-SRDQIPVACKDSVAILFMFDLTSRCTLNSVISWYQQARKSNQTAIPVMVGTK 220

Query: 196 FDDFIQLPIELQWAIASE 213
           FD+FIQLPI+LQW IAS+
Sbjct: 221 FDEFIQLPIDLQWTIASQ 237

BLAST of MC02g0533 vs. TAIR 10
Match: AT5G54840.2 (Ras-related small GTP-binding family protein )

HSP 1 Score: 155.2 bits (391), Expect = 7.2e-38
Identity = 83/156 (53.21%), Postives = 103/156 (66.03%), Query Frame = 0

Query: 36  LKKPNTYRMVPEATASASGNV---ADDGFAAETMTPLSHNHGLDRDLVSLKISLLGDSQI 95
           ++KP  YR +    +S +G++    DD     T++        D DLVSLKISLLGD Q 
Sbjct: 53  IEKPILYRRIVHKISSTAGDIFDGDDDTTTEPTVSGRRFGSSSDSDLVSLKISLLGDCQT 112

Query: 96  GKTSFLEKYVGNEMEDGSRAQKGLNLMDKTLMVRGARISYSIWEVGGDRK-SLDYIPTAC 155
           GKT+F+ KYVG+E         GLNLMDKT  V+G  IS+SIW+VGGD K S D+IP AC
Sbjct: 113 GKTTFVVKYVGDE-NQSFLEMTGLNLMDKTFYVQGVTISFSIWDVGGDEKRSKDHIPIAC 172

Query: 156 KDSVAILFMFDLTSRRTLNSILRWYRQALKWNQTAI 188
           KD+VAILFMFDLTSR TLNS+  WY QA KWN+  +
Sbjct: 173 KDAVAILFMFDLTSRSTLNSVFGWYSQARKWNKVRL 207

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P870271.4e-4948.66Septum-promoting GTP-binding protein 1 OS=Schizosaccharomyces pombe (strain 972 ... [more]
P389879.2e-3841.49Protein TEM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 ... [more]
P075601.9e-1427.60Ras-related protein SEC4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c... [more]
Q9JKM74.2e-1428.86Ras-related protein Rab-37 OS=Mus musculus OX=10090 GN=Rab37 PE=1 SV=2[more]
Q9FIF91.6e-1326.92Ras-related protein RABA2d OS=Arabidopsis thaliana OX=3702 GN=RABA2D PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022140324.14.84e-168100.00septum-promoting GTP-binding protein 1 [Momordica charantia] >XP_022140325.1 sep... [more]
KAG7034967.12.06e-14678.47spg1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023534173.18.87e-14678.10septum-promoting GTP-binding protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_022971065.11.19e-14578.10septum-promoting GTP-binding protein 1-like [Cucurbita maxima][more]
XP_022947099.14.82e-14578.10septum-promoting GTP-binding protein 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1CFF32.34e-168100.00septum-promoting GTP-binding protein 1 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1I4P45.75e-14678.10septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1G5T02.33e-14578.10septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JFU66.72e-13775.75septum-promoting GTP-binding protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1FYS53.87e-13675.00septum-promoting GTP-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT3G21700.31.4e-8177.08Ras-related small GTP-binding family protein [more]
AT3G21700.13.4e-8077.08Ras-related small GTP-binding family protein [more]
AT5G54840.11.8e-7660.85Ras-related small GTP-binding family protein [more]
AT3G21700.22.4e-4971.01Ras-related small GTP-binding family protein [more]
AT5G54840.27.2e-3853.21Ras-related small GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00449RASTRNSFRMNGcoord: 82..103
score: 34.52
coord: 184..197
score: 31.92
coord: 123..145
score: 17.59
coord: 224..246
score: 25.48
NoneNo IPR availableSMARTSM00175rab_sub_5coord: 82..249
e-value: 8.4E-7
score: -24.8
NoneNo IPR availablePANTHERPTHR47978:SF29MONOMERIC G-PROTEINcoord: 44..265
NoneNo IPR availablePANTHERPTHR47978FAMILY NOT NAMEDcoord: 44..265
NoneNo IPR availablePROSITEPS51419RABcoord: 76..266
score: 11.824186
IPR017231Small GTPase Tem1/Spg1PIRSFPIRSF037527GTPase_Tem1coord: 5..266
e-value: 2.3E-93
score: 309.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 76..256
e-value: 8.9E-31
score: 108.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 82..257
IPR001806Small GTPasePFAMPF00071Rascoord: 83..246
e-value: 3.7E-20
score: 72.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0533.1MC02g0533.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0090404 pollen tube tip
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding