MC02g0528 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0528
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Isocitrate lyase
Location	MC02: 4302875 .. 4305332 (+)
RNA-Seq Expression	MC02g0528
Synteny	MC02g0528

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGATAATGGAAGAAGAAGGAAGATTCGAAGCGGAAGTTGCAGAGGTGGAGGCATGGTGGAACTCAGAGAGGTTCAAGCTGACGCGGCGGCCGTACAAGGCCAGAGACGTGGTCTCGTTACGAGGCAGCCTAGGACAGAGCTACGCCTCAAACGAATTGGCCAAAAAGCTATGGCGGACGCTCAAAACCCACCAAGCCAACGGCACGGCCTCCAGAACATTCGGCGCCCTCGACCCGGTCCAAGTCACCATGATGGCTAAGCATTTGGACACCATCTACGTCTCCGGCTGGCAGTGCTCCTCCACCCACACCTCCACCAACGAGCCAGGCCCGGACCTCGCCGATTACCCATACGACACCGTTCCCAACAAGGTCGAGCATCTGTTCTTCGCGCAGCAGTACCACGACAGGAAGCAGAGGGAGGCCAGGATGAGTATGAGCAGAGAGGAAAGAGCCAGAACCCCTTATGTTGACTATCTCAAACCCATCATCGCCGACGGCGACACCGGCTTCGGCGGCACCACCGCCACGGTTAAGCTCTGCAAGCTATTCGTGGAGCGCGGTGCGGCCGGGGTCCACATTGAGGATCAGTCCTCTGTGACCAAAAAGTGCGGTCATATGGCCGGGAAAGTGCTCGTGGCGATCAGCGAGCACATCAACCGGCTCGTGGCGGCTCGATTGCAGTTCGATGTAATGGGGGTTGAGACGGTTCTCGTGGCTCGAACCGACGCCGTCGCGGCCACTTTGATCCAGACGAATGTTGACAGCAGAGACCACCAGTTCATTTTGGGCGCGACAAACCCGAATGTCAGAGGGAAGAGCTTGGCAGGTGTTCTGGCCGAAGCCATGGCGGCCGGGAAAACAGGGGCCGAGCTTCAGGCGCTTGAAGATCAATGGATTTCAATGGCGCAACTGAAGACATTCTCCGAATGCGTCACGGACGCAATCATGAATACGAACGCCACCGAGAATGAAAAGAGGAGGAAATTGGACGAGTGGATGAACCATTCGAGTTACGAGAAATGTATCTCGAACGAACAGGGTCGGGAAATCGCGGAGAAATTAGGGCTGAAGAACCTATTCTGGGACTGGGATTTGCCGAGAACAAGAGAGGGGTTTTACAGGTTCAAAGGGTCGGTAATGGCGGCCATTGTTCGTGGGTGGGCTTTCGCCCCACACGCGGACCTAATATGGATGGAAACGTCGAGCCCGGACTTGGTAGAGTGCACGACATTCGCGAAAGGGGTGAAATCGATGCACCCAGAAATGATGCTGGCTTACAATCTGTCGCCATCGTTCAACTGGGACGCATCGGGAATGAGCGACAAGCAGATGGAGGAGTTCATCCCGAGAATAGCGAGGCTGGGATTCTGCTGGCAGTTCATAACGCTGGCGGGGTTCCATGCGGACGCGCTGGTGGTGGACACGTTCGCCAGAGACTACGCGAGAAGGGGAATGCTGGCTTACGTGGAGAGAATCCAGAGGGAAGAGAGAAGCAATGGAGTGGACACGCTGGCCCATCAGAAATGGTCCGGCGCCAACTACTACGACCGCTATTTGAAGACGGTTCAAGGGGGCATTTCGTCCACCGCCGCCATGGGAAAAGGCAAGCCAATCCAATCCAAACCAAACGAATTAGTATTATGATTTTATGAATTATCCAACTAACTTAACGAATTAGTATTGGTTTTGGGTTGCAGGAGTGACGGAGGAGCAGTTCAAGGAGTCGTGGACGAGGCAGGGAGCAGGGGACTTGGGAGAAGAAGGGACCGTGGTGGTGGCCAAGTCAAGGATGTGATTCTGAGCTTTGGTTGGATTGGAGAAGGGGAACACAATAATATGGCGTTGCATATTTTATTTCGTTGGTTGTTTGGGTCAACCCGTTTTTGGTGTCCAATAACCAAACTTTATTTATTTTCTACGTTTGTTGGTTAATGTGCTTCAATTTTAGTTACCAGCATTATGTATTGTCTTGTCGTCGTATTCATATTTTATTATCTGTTCAGTGAAGGCATCTTTTAAGGTTGGAAATCTAATCTTGAAGAACTATAGCAAAGAGGTCTTGCTTTTCTCGGAAGGCAAACTTCAAAAACTATAGAAAGAAAAACGGCGAAACTGAGTTCACTTTCTGGTACCATTTACTTCCATGGCTTCAAAACTGAGAGGACGATTATGATTGCTATAACCAGGAGTAGAATGATGGTAATCATAATGCGATTTCTTGATTTCCTCTGTAAGCTCTTTGCCGTCAGGAGTGTATCTGTTCCCGTCGTCACCTATGTCAAAAGAAGTTGCAGCAGTTTCAAAACAACTTCCTACTTGATCAAGTCAACTATGAAAAAACTAGGACCAATCAAAATAATTATAAGGACACCCTCCACCATGTAAACTGTTGTGCCACATTGACCAAATAGAGGTTCAAGTTCTGAGAAAATTTAGGCAGCAACCTGATTTTCTA

mRNA sequence

CAGATAATGGAAGAAGAAGGAAGATTCGAAGCGGAAGTTGCAGAGGTGGAGGCATGGTGGAACTCAGAGAGGTTCAAGCTGACGCGGCGGCCGTACAAGGCCAGAGACGTGGTCTCGTTACGAGGCAGCCTAGGACAGAGCTACGCCTCAAACGAATTGGCCAAAAAGCTATGGCGGACGCTCAAAACCCACCAAGCCAACGGCACGGCCTCCAGAACATTCGGCGCCCTCGACCCGGTCCAAGTCACCATGATGGCTAAGCATTTGGACACCATCTACGTCTCCGGCTGGCAGTGCTCCTCCACCCACACCTCCACCAACGAGCCAGGCCCGGACCTCGCCGATTACCCATACGACACCGTTCCCAACAAGGTCGAGCATCTGTTCTTCGCGCAGCAGTACCACGACAGGAAGCAGAGGGAGGCCAGGATGAGTATGAGCAGAGAGGAAAGAGCCAGAACCCCTTATGTTGACTATCTCAAACCCATCATCGCCGACGGCGACACCGGCTTCGGCGGCACCACCGCCACGGTTAAGCTCTGCAAGCTATTCGTGGAGCGCGGTGCGGCCGGGGTCCACATTGAGGATCAGTCCTCTGTGACCAAAAAGTGCGGTCATATGGCCGGGAAAGTGCTCGTGGCGATCAGCGAGCACATCAACCGGCTCGTGGCGGCTCGATTGCAGTTCGATGTAATGGGGGTTGAGACGGTTCTCGTGGCTCGAACCGACGCCGTCGCGGCCACTTTGATCCAGACGAATGTTGACAGCAGAGACCACCAGTTCATTTTGGGCGCGACAAACCCGAATGTCAGAGGGAAGAGCTTGGCAGGTGTTCTGGCCGAAGCCATGGCGGCCGGGAAAACAGGGGCCGAGCTTCAGGCGCTTGAAGATCAATGGATTTCAATGGCGCAACTGAAGACATTCTCCGAATGCGTCACGGACGCAATCATGAATACGAACGCCACCGAGAATGAAAAGAGGAGGAAATTGGACGAGTGGATGAACCATTCGAGTTACGAGAAATGTATCTCGAACGAACAGGGTCGGGAAATCGCGGAGAAATTAGGGCTGAAGAACCTATTCTGGGACTGGGATTTGCCGAGAACAAGAGAGGGGTTTTACAGGTTCAAAGGGTCGGTAATGGCGGCCATTGTTCGTGGGTGGGCTTTCGCCCCACACGCGGACCTAATATGGATGGAAACGTCGAGCCCGGACTTGGTAGAGTGCACGACATTCGCGAAAGGGGTGAAATCGATGCACCCAGAAATGATGCTGGCTTACAATCTGTCGCCATCGTTCAACTGGGACGCATCGGGAATGAGCGACAAGCAGATGGAGGAGTTCATCCCGAGAATAGCGAGGCTGGGATTCTGCTGGCAGTTCATAACGCTGGCGGGGTTCCATGCGGACGCGCTGGTGGTGGACACGTTCGCCAGAGACTACGCGAGAAGGGGAATGCTGGCTTACGTGGAGAGAATCCAGAGGGAAGAGAGAAGCAATGGAGTGGACACGCTGGCCCATCAGAAATGGTCCGGCGCCAACTACTACGACCGCTATTTGAAGACGGTTCAAGGGGGCATTTCGTCCACCGCCGCCATGGGAAAAGGCAGAGTGACGGAGGAGCAGTTCAAGGAGTCGTGGACGAGGCAGGGAGCAGGGGACTTGGGAGAAGAAGGGACCGTGGTGGTGGCCAAGTCAAGGATGTGATTCTGAGCTTTGGTTGGATTGGAGAAGGGGAACACAATAATATGGCGTTGCATATTTTATTTCGTTGGTTGTTTGGGTCAACCCGTTTTTGGTGTCCAATAACCAAACTTTATTTATTTTCTACGTTTGTTGGTTAATGTGCTTCAATTTTAGTTACCAGCATTATGTATTGTCTTGTCGTCGTATTCATATTTTATTATCTGTTCAGTGAAGGCATCTTTTAAGGTTGGAAATCTAATCTTGAAGAACTATAGCAAAGAGGTCTTGCTTTTCTCGGAAGGCAAACTTCAAAAACTATAGAAAGAAAAACGGCGAAACTGAGTTCACTTTCTGGTACCATTTACTTCCATGGCTTCAAAACTGAGAGGACGATTATGATTGCTATAACCAGGAGTAGAATGATGGTAATCATAATGCGATTTCTTGATTTCCTCTGTAAGCTCTTTGCCGTCAGGAGTGTATCTGTTCCCGTCGTCACCTATGTCAAAAGAAGTTGCAGCAGTTTCAAAACAACTTCCTACTTGATCAAGTCAACTATGAAAAAACTAGGACCAATCAAAATAATTATAAGGACACCCTCCACCATGTAAACTGTTGTGCCACATTGACCAAATAGAGGTTCAAGTTCTGAGAAAATTTAGGCAGCAACCTGATTTTCTA

Coding sequence (CDS)

CAGATAATGGAAGAAGAAGGAAGATTCGAAGCGGAAGTTGCAGAGGTGGAGGCATGGTGGAACTCAGAGAGGTTCAAGCTGACGCGGCGGCCGTACAAGGCCAGAGACGTGGTCTCGTTACGAGGCAGCCTAGGACAGAGCTACGCCTCAAACGAATTGGCCAAAAAGCTATGGCGGACGCTCAAAACCCACCAAGCCAACGGCACGGCCTCCAGAACATTCGGCGCCCTCGACCCGGTCCAAGTCACCATGATGGCTAAGCATTTGGACACCATCTACGTCTCCGGCTGGCAGTGCTCCTCCACCCACACCTCCACCAACGAGCCAGGCCCGGACCTCGCCGATTACCCATACGACACCGTTCCCAACAAGGTCGAGCATCTGTTCTTCGCGCAGCAGTACCACGACAGGAAGCAGAGGGAGGCCAGGATGAGTATGAGCAGAGAGGAAAGAGCCAGAACCCCTTATGTTGACTATCTCAAACCCATCATCGCCGACGGCGACACCGGCTTCGGCGGCACCACCGCCACGGTTAAGCTCTGCAAGCTATTCGTGGAGCGCGGTGCGGCCGGGGTCCACATTGAGGATCAGTCCTCTGTGACCAAAAAGTGCGGTCATATGGCCGGGAAAGTGCTCGTGGCGATCAGCGAGCACATCAACCGGCTCGTGGCGGCTCGATTGCAGTTCGATGTAATGGGGGTTGAGACGGTTCTCGTGGCTCGAACCGACGCCGTCGCGGCCACTTTGATCCAGACGAATGTTGACAGCAGAGACCACCAGTTCATTTTGGGCGCGACAAACCCGAATGTCAGAGGGAAGAGCTTGGCAGGTGTTCTGGCCGAAGCCATGGCGGCCGGGAAAACAGGGGCCGAGCTTCAGGCGCTTGAAGATCAATGGATTTCAATGGCGCAACTGAAGACATTCTCCGAATGCGTCACGGACGCAATCATGAATACGAACGCCACCGAGAATGAAAAGAGGAGGAAATTGGACGAGTGGATGAACCATTCGAGTTACGAGAAATGTATCTCGAACGAACAGGGTCGGGAAATCGCGGAGAAATTAGGGCTGAAGAACCTATTCTGGGACTGGGATTTGCCGAGAACAAGAGAGGGGTTTTACAGGTTCAAAGGGTCGGTAATGGCGGCCATTGTTCGTGGGTGGGCTTTCGCCCCACACGCGGACCTAATATGGATGGAAACGTCGAGCCCGGACTTGGTAGAGTGCACGACATTCGCGAAAGGGGTGAAATCGATGCACCCAGAAATGATGCTGGCTTACAATCTGTCGCCATCGTTCAACTGGGACGCATCGGGAATGAGCGACAAGCAGATGGAGGAGTTCATCCCGAGAATAGCGAGGCTGGGATTCTGCTGGCAGTTCATAACGCTGGCGGGGTTCCATGCGGACGCGCTGGTGGTGGACACGTTCGCCAGAGACTACGCGAGAAGGGGAATGCTGGCTTACGTGGAGAGAATCCAGAGGGAAGAGAGAAGCAATGGAGTGGACACGCTGGCCCATCAGAAATGGTCCGGCGCCAACTACTACGACCGCTATTTGAAGACGGTTCAAGGGGGCATTTCGTCCACCGCCGCCATGGGAAAAGGCAGAGTGACGGAGGAGCAGTTCAAGGAGTCGTGGACGAGGCAGGGAGCAGGGGACTTGGGAGAAGAAGGGACCGTGGTGGTGGCCAAGTCAAGGATGTGA

Protein sequence

QIMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVARTDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWISMAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHPEMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDYARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEEQFKESWTRQGAGDLGEEGTVVVAKSRM

Homology

[Close]

BLAST of MC02g0528 vs. ExPASy Swiss-Prot
Match: P49296 (Isocitrate lyase OS=Cucumis sativus OX=3659 PE=3 SV=1)

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 546/567 (96.30%), Postives = 559/567 (98.59%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVD RDHQFILGATNPN+RGKSLAG LAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKG+KS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGMKSIHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E MLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 569
           QFKESWTR+GA +LGEEG VVVAKSRM
Sbjct: 551 QFKESWTREGAVNLGEEGNVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. ExPASy Swiss-Prot
Match: P93110 (Isocitrate lyase OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 1106.3 bits (2860), Expect = 0.0e+00
Identity = 545/567 (96.12%), Postives = 560/567 (98.77%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVDSRDHQFILGATNPN+RGKSLAGVLAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMN+N TE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 569
           QFKESWTR GAG+LGEEG+VVVAKSRM
Sbjct: 551 QFKESWTRAGAGNLGEEGSVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. ExPASy Swiss-Prot
Match: P17069 (Isocitrate lyase OS=Gossypium hirsutum OX=3635 PE=2 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 2.3e-304
Identity = 504/567 (88.89%), Postives = 544/567 (95.94%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFE EVAEV+AWWNSERFKLTRRPY ARDVV+LRGSL QSY SNE+AKKLW TL
Sbjct: 11  IMEEEGRFETEVAEVQAWWNSERFKLTRRPYSARDVVALRGSLKQSYGSNEMAKKLWTTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVD+RDHQFILGATNPN+RGKSLA +LAE MAAGK G +LQA+ED W++
Sbjct: 251 TDAVAATLIQTNVDTRDHQFILGATNPNLRGKSLANMLAEGMAAGKNGPQLQAIEDNWLA 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           +AQLKTFSECV DAI + N TE+EKRR+++EWMNHSSY+KC+SNEQ REIAE+LGL+NLF
Sbjct: 311 IAQLKTFSECVMDAIKSMNITEDEKRRRMNEWMNHSSYDKCLSNEQAREIAERLGLQNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRF+GSVMAAIVRGWAFAPHADLIWMETSSPD+VECT FA+GVKSMHP
Sbjct: 371 WDWDLPRTREGFYRFRGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTRFAEGVKSMHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGM+D+ M +FIPRIA+LGFCWQFITLAGFHADALV DTFARD+
Sbjct: 431 EIMLAYNLSPSFNWDASGMTDEHMRDFIPRIAKLGFCWQFITLAGFHADALVTDTFARDF 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVE+IQREER+NGVDTLAHQKWSGAN+YDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVEKIQREERNNGVDTLAHQKWSGANFYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 569
           QFKE+WTR GAG++G EG +VVAK+RM
Sbjct: 551 QFKETWTRPGAGNIGSEGNLVVAKARM 576

[Close]

BLAST of MC02g0528 vs. ExPASy Swiss-Prot
Match: P15479 (Isocitrate lyase OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 3.8e-299
Identity = 499/567 (88.01%), Postives = 536/567 (94.53%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY ARDVV+LRG+L QSYASNELAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTARDVVALRGNLKQSYASNELAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETLLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDA AA LIQ+NVD+RDHQFILG TNPN+RGKSLA +LA  MA GKTGAELQA ED W++
Sbjct: 251 TDAEAANLIQSNVDTRDHQFILGVTNPNLRGKSLATLLATGMANGKTGAELQATEDNWLA 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTF ECV DAI N NA E+EKRR+++EWMNH+SY+KC+S EQGREIA+++GLKNLF
Sbjct: 311 MAQLKTFPECVMDAIKNMNAGEDEKRRRMNEWMNHTSYDKCLSYEQGREIADRMGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAA+VRG AFAPHAD+IWMET+ PD  ECT FA+GVKSMHP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAVVRGRAFAPHADIIWMETAKPDFAECTAFAEGVKSMHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGM+D+QM +FIPRIARLGFCWQFITL GFHADALV+DTFA+DY
Sbjct: 431 EIMLAYNLSPSFNWDASGMTDEQMRDFIPRIARLGFCWQFITLGGFHADALVIDTFAKDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERKNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 569
           QFKE+WTR GA ++G  G+ VVAK+RM
Sbjct: 551 QFKETWTRPGAMEMGSAGSEVVAKARM 576

[Close]

BLAST of MC02g0528 vs. ExPASy Swiss-Prot
Match: P49297 (Isocitrate lyase OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 2.5e-290
Identity = 482/567 (85.01%), Postives = 533/567 (94.00%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEE RFEAEVAEV+AWWN+ERF+LT+R Y ARDVV+LRG++ QSYASNELA+KLWRTL
Sbjct: 11  IMEEERRFEAEVAEVQAWWNTERFRLTKRAYSARDVVALRGTMRQSYASNELAQKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSM REERARTP++DYLKPIIADGDTGFGG TATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMCREERARTPFIDYLKPIIADGDTGFGGATATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMG ETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGTETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVD+RDHQFILGA+NPN++GK LA  L+EAMAAGKTG ELQA+ED W+ 
Sbjct: 251 TDAVAATLIQTNVDTRDHQFILGASNPNLKGKGLATHLSEAMAAGKTGPELQAIEDNWLG 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MA+LKTFS+CVTDAI   N  E EK+RKL+EWMNHSSYEKC+S+EQ RE+AE+LGL NLF
Sbjct: 311 MAELKTFSQCVTDAIKKMNLAEYEKQRKLNEWMNHSSYEKCLSHEQAREVAERLGLPNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRF+GSV AAIVRGWAFA + DL+WMETSSPD+VECT F++GVK++ P
Sbjct: 371 WDWDLPRTREGFYRFQGSVEAAIVRGWAFAEYCDLVWMETSSPDMVECTKFSQGVKTLRP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGM+D QM +FIPRIA+LG+CWQFITLAGFHADAL+VDTFA+D+
Sbjct: 431 ELMLAYNLSPSFNWDASGMNDNQMMDFIPRIAKLGYCWQFITLAGFHADALIVDTFAKDF 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVE+IQREERSNGVDTLAHQKWSGANYYDR L+TVQGGI+STAAMGKG VTEE
Sbjct: 491 ARRGMLAYVEKIQREERSNGVDTLAHQKWSGANYYDRVLRTVQGGITSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 569
           QF+E WTR GA +LG +G+VV+AK+RM
Sbjct: 551 QFEEKWTRTGATNLG-DGSVVIAKARM 575

[Close]

BLAST of MC02g0528 vs. NCBI nr
Match: XP_038900496.1 (isocitrate lyase [Benincasa hispida])

HSP 1 Score: 1105 bits (2857), Expect = 0.0
Identity = 548/567 (96.65%), Postives = 560/567 (98.77%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           K HQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KNHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVDSRDHQFILGATNPN+RGKSLAGVLAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMNTNATE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E MLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR+GA +LGEEG VVVAKSRM
Sbjct: 551 QFKESWTREGAVNLGEEGNVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. NCBI nr
Match: P49296.1 (RecName: Full=Isocitrate lyase; Short=ICL; AltName: Full=Isocitrase; AltName: Full=Isocitratsysase [Cucumis sativus] >CAA84632.1 isocitrate lyase [Cucumis sativus])

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 546/567 (96.30%), Postives = 559/567 (98.59%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVD RDHQFILGATNPN+RGKSLAG LAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKG+KS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGMKSIHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E MLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR+GA +LGEEG VVVAKSRM
Sbjct: 551 QFKESWTREGAVNLGEEGNVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. NCBI nr
Match: XP_004151880.1 (isocitrate lyase [Cucumis sativus] >KGN63267.1 hypothetical protein Csa_022060 [Cucumis sativus])

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 545/567 (96.12%), Postives = 558/567 (98.41%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLV +SEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVGVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVD RDHQFILGATNPN+RGKSLAG LAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKG+KS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGMKSIHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E MLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR+GA +LGEEG VVVAKSRM
Sbjct: 551 QFKESWTREGAVNLGEEGNVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. NCBI nr
Match: KAG6570876.1 (hypothetical protein SDJN03_29791, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 546/569 (95.96%), Postives = 561/569 (98.59%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVDSRDHQFILGATNPN+RGKSLAGVLAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMN+N TE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG--RVT 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG  RVT
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGKQRVT 550

Query: 542 EEQFKESWTRQGAGDLGEEGTVVVAKSRM 568
           EEQFKESWTR GAG+LGEEG+VVVAKSRM
Sbjct: 551 EEQFKESWTRAGAGNLGEEGSVVVAKSRM 579

[Close]

BLAST of MC02g0528 vs. NCBI nr
Match: XP_022986274.1 (isocitrate lyase [Cucurbita maxima] >P93110.1 RecName: Full=Isocitrate lyase; Short=ICL; AltName: Full=Isocitrase; AltName: Full=Isocitratsysase [Cucurbita maxima] >BAA11320.1 Isocitrate Lyase [Cucurbita hybrid cultivar])

HSP 1 Score: 1101 bits (2847), Expect = 0.0
Identity = 545/567 (96.12%), Postives = 560/567 (98.77%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVDSRDHQFILGATNPN+RGKSLAGVLAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMN+N TE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR GAG+LGEEG+VVVAKSRM
Sbjct: 551 QFKESWTRAGAGNLGEEGSVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. ExPASy TrEMBL
Match: A0A0A0LR30 (Isocitrate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G420990 PE=3 SV=1)

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 545/567 (96.12%), Postives = 558/567 (98.41%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLV +SEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVGVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVD RDHQFILGATNPN+RGKSLAG LAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKG+KS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGMKSIHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E MLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR+GA +LGEEG VVVAKSRM
Sbjct: 551 QFKESWTREGAVNLGEEGNVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. ExPASy TrEMBL
Match: A0A6J1JFM0 (Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111484071 PE=3 SV=1)

HSP 1 Score: 1101 bits (2847), Expect = 0.0
Identity = 545/567 (96.12%), Postives = 560/567 (98.77%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVDSRDHQFILGATNPN+RGKSLAGVLAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMN+N TE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR GAG+LGEEG+VVVAKSRM
Sbjct: 551 QFKESWTRAGAGNLGEEGSVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. ExPASy TrEMBL
Match: A0A1S3C1U5 (Isocitrate lyase OS=Cucumis melo OX=3656 GN=LOC103495946 PE=3 SV=1)

HSP 1 Score: 1100 bits (2845), Expect = 0.0
Identity = 545/567 (96.12%), Postives = 559/567 (98.59%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVD RDHQFILGATNPN+RGKSLAGVLAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMNT ATE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTIATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSIHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E MLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR+GA +LGEEG VVVAKSRM
Sbjct: 551 QFKESWTREGAVNLGEEGNVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. ExPASy TrEMBL
Match: A0A6J1FXZ6 (Isocitrate lyase OS=Cucurbita moschata OX=3662 GN=LOC111448305 PE=3 SV=1)

HSP 1 Score: 1099 bits (2843), Expect = 0.0
Identity = 544/567 (95.94%), Postives = 559/567 (98.59%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+AWWNSERFKLTRRPY A+DVVSLRGSL QSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQTNVD RDHQFILGATNPN+RGKSLAGVLAEAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDGRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMN+N TE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR GAG+LGEEG+VVVAKSRM
Sbjct: 551 QFKESWTRAGAGNLGEEGSVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. ExPASy TrEMBL
Match: A0A6J1I0N5 (Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111469780 PE=3 SV=1)

HSP 1 Score: 1088 bits (2813), Expect = 0.0
Identity = 537/567 (94.71%), Postives = 554/567 (97.71%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV AWWNSERFKLTRRPY A+DVVSLRGSL QSYASNELAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVHAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASNELAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAV ATLIQTNVDSRDHQFILGATNPN+RGKSLAG L+EAMAAGKTGAELQALEDQWIS
Sbjct: 251 TDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAELQALEDQWIS 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
           MAQLKTFSECVTDAIMN NATENEKRRKLDEWM+ SSYEKC+SNEQGREI EKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNMNATENEKRRKLDEWMSQSSYEKCLSNEQGREIVEKLGLKNLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPD+VECT+FAKGVKS+HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTSFAKGVKSVHP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 ELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGML YVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMG+G VTEE
Sbjct: 491 ARRGMLGYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGRG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 568
           QFKESWTR+GA +LGE G VVVAKSRM
Sbjct: 551 QFKESWTREGAVNLGEGGNVVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. TAIR 10
Match: AT3G21720.1 (isocitrate lyase )

HSP 1 Score: 981.5 bits (2536), Expect = 2.9e-286
Identity = 477/567 (84.13%), Postives = 519/567 (91.53%), Query Frame = 0


Query: 2   IMEEEGRFEAEVAEVEAWWNSERFKLTRRPYKARDVVSLRGSLGQSYASNELAKKLWRTL 61
           IMEEEGRFEAEVAEV+ WW+SERFKLTRRPY ARDVV+LRG L Q YASNE+AKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQTWWSSERFKLTRRPYTARDVVALRGHLKQGYASNEMAKKLWRTL 70

Query: 62  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 121
           K+HQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KSHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 122 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 181
           PNKVEHLFFAQQYHDRKQREARMSMSREER +TP+VDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERTKTPFVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 182 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETVLVAR 241
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMG ETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGTETVLVAR 250

Query: 242 TDAVAATLIQTNVDSRDHQFILGATNPNVRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 301
           TDAVAATLIQ+N+D+RDHQFILGATNP++RGKSL+ +LAE M  GK G  LQ++EDQW+ 
Sbjct: 251 TDAVAATLIQSNIDARDHQFILGATNPSLRGKSLSSLLAEGMTVGKNGPALQSIEDQWLG 310

Query: 302 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 361
            A L TFSE V  AI   N  ENEK ++L EW+ H+ YE C+SNEQGR +A KLG+ +LF
Sbjct: 311 SAGLMTFSEAVVQAIKRMNLNENEKNQRLSEWLTHARYENCLSNEQGRVLAAKLGVTDLF 370

Query: 362 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSMHP 421
           WDWDLPRTREGFYRF+GSV AA+VRGWAFA  AD+IWMET+SPDL ECT FA+G+KS  P
Sbjct: 371 WDWDLPRTREGFYRFQGSVAAAVVRGWAFAQIADIIWMETASPDLNECTQFAEGIKSKTP 430

Query: 422 EMMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 481
           E+MLAYNLSPSFNWDASGM+D+QM EFIPRIARLG+CWQFITLAGFHADALVVDTFA+DY
Sbjct: 431 EVMLAYNLSPSFNWDASGMTDQQMVEFIPRIARLGYCWQFITLAGFHADALVVDTFAKDY 490

Query: 482 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGRVTEE 541
           ARRGMLAYVERIQREER++GVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG VTEE
Sbjct: 491 ARRGMLAYVERIQREERTHGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG-VTEE 550

Query: 542 QFKESWTRQGAGDLGEEGTVVVAKSRM 569
           QFKESWTR GA  +GE  ++VVAKSRM
Sbjct: 551 QFKESWTRPGADGMGEGTSLVVAKSRM 576

[Close]

BLAST of MC02g0528 vs. TAIR 10
Match: AT1G21440.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.8e-09
Identity = 37/86 (43.02%), Postives = 50/86 (58.14%), Query Frame = 0


Query: 162 PIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINR 221
           PIIAD DTG G      +  K  +  GAAG  +EDQ +  K+CGHM GK ++   EH  +
Sbjct: 115 PIIADADTGGGNALNVQRTVKDLIAAGAAGCFLEDQ-AWPKRCGHMRGKEVIPAEEHAAK 174

Query: 222 LVAARLQFDVMG-VETVLVARTDAVA 247
           + +AR   D +G  +  L+ARTDA A
Sbjct: 175 IASAR---DAIGDADFFLIARTDARA 196

[Close]

BLAST of MC02g0528 vs. TAIR 10
Match: AT1G77060.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 4.0e-09
Identity = 38/88 (43.18%), Postives = 50/88 (56.82%), Query Frame = 0


Query: 162 PIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINR 221
           PIIAD DTG G      +  K  +  GAAG  +EDQ +  KKCGHM GK ++   EH  +
Sbjct: 117 PIIADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQ-AWPKKCGHMRGKQVIPAEEHAAK 176

Query: 222 LVAARLQFDVMG-VETVLVARTDAVAAT 249
           + +AR   D +G  +  LVARTD  A +
Sbjct: 177 IASAR---DAIGDSDFFLVARTDVRATS 200

The following BLAST results are available for this feature:

BLAST of MC02g0528 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49296	0.0e+00	96.30	Isocitrate lyase OS=Cucumis sativus OX=3659 PE=3 SV=1	[more]
P93110	0.0e+00	96.12	Isocitrate lyase OS=Cucurbita maxima OX=3661 PE=2 SV=1	[more]
P17069	2.3e-304	88.89	Isocitrate lyase OS=Gossypium hirsutum OX=3635 PE=2 SV=1	[more]
P15479	3.8e-299	88.01	Isocitrate lyase OS=Ricinus communis OX=3988 PE=2 SV=1	[more]
P49297	2.5e-290	85.01	Isocitrate lyase OS=Solanum lycopersicum OX=4081 PE=2 SV=1	[more]

BLAST of MC02g0528 vs. NCBI nr


Match Name	Identity	Description
XP_038900496.1	96.65	isocitrate lyase [Benincasa hispida]	[more]
P49296.1	96.30	RecName: Full=Isocitrate lyase; Short=ICL; AltName: Full=Isocitrase; AltName: Fu...	[more]
XP_004151880.1	96.12	isocitrate lyase [Cucumis sativus] >KGN63267.1 hypothetical protein Csa_022060 [...	[more]
KAG6570876.1	95.96	hypothetical protein SDJN03_29791, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022986274.1	96.12	isocitrate lyase [Cucurbita maxima] >P93110.1 RecName: Full=Isocitrate lyase; Sh...	[more]

BLAST of MC02g0528 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A0A0LR30	96.12	Isocitrate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G420990 PE=3 SV=1	[more]
A0A6J1JFM0	96.12	Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111484071 PE=3 SV=1	[more]
A0A1S3C1U5	96.12	Isocitrate lyase OS=Cucumis melo OX=3656 GN=LOC103495946 PE=3 SV=1	[more]
A0A6J1FXZ6	95.94	Isocitrate lyase OS=Cucurbita moschata OX=3662 GN=LOC111448305 PE=3 SV=1	[more]
A0A6J1I0N5	94.71	Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111469780 PE=3 SV=1	[more]

BLAST of MC02g0528 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21720.1	2.9e-286	84.13	isocitrate lyase	[more]
AT1G21440.1	1.8e-09	43.02	Phosphoenolpyruvate carboxylase family protein	[more]
AT1G77060.1	4.0e-09	43.18	Phosphoenolpyruvate carboxylase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006254	Isocitrate lyase	PFAM	PF00463	ICL	coord: 12..543 e-value: 2.0E-274 score: 911.0
IPR006254	Isocitrate lyase	TIGRFAM	TIGR01346	TIGR01346	coord: 13..543 e-value: 1.4E-262 score: 869.8
IPR006254	Isocitrate lyase	PIRSF	PIRSF001362	ICL	coord: 3..324 e-value: 1.5E-62 score: 209.6 coord: 359..546 e-value: 1.1E-53 score: 180.3
IPR006254	Isocitrate lyase	PANTHER	PTHR21631	ISOCITRATE LYASE/MALATE SYNTHASE	coord: 3..546
None	No IPR available	GENE3D	1.10.10.850		coord: 253..368 e-value: 1.2E-243 score: 811.1
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 8..526 e-value: 1.2E-243 score: 811.1
IPR018523	Isocitrate lyase/phosphorylmutase, conserved site	PROSITE	PS00161	ISOCITRATE_LYASE	coord: 202..207
IPR039556	ICL/PEPM domain	CDD	cd00377	ICL_PEPM	coord: 66..480 e-value: 2.09029E-45 score: 158.42
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 3..526

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g0528.1	MC02g0528.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006097	glyoxylate cycle
biological_process	GO:0006099	tricarboxylic acid cycle
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0009514	glyoxysome
molecular_function	GO:0004451	isocitrate lyase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003824	catalytic activity