MC02g0509 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0509
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptioncoumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like
LocationMC02: 4145318 .. 4146769 (-)
RNA-Seq ExpressionMC02g0509
SyntenyMC02g0509
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATGCCAACATCGTAAATGAGTGCTGGGTTTCTCCACCCGCAGGCTCAGTTGCCACCACAAATCTCCCCTTGTCTTTCTTCGACATGCTGTGGATTCCTAACCCCCCGATGCGACGCTTTTTCTTCTACGACTTCCCCCACTCCACACACCACTTCATGGACGCCATTTTTCCCACTCTCACACGCTCCCTTTCCCTCGTTCTCCAACACTTTTTTCCTCTCGCCGGAAATCTCATCTTCCCTTCTCAACCCCACACCCACAAGCCCCACATTCTCTACACAACGGCCCACGACTCGGTTCCCCTTACAGTGGCCGAGTCACCTCAAACTCAAAGTGGGTGCGACGTTTTCAACCGCCTCATCGCAGATTACCCCCGCCACGCCAAACACTTGTACCCCTTTGTTCCGGGCTTGCCCAAGCCACGTGTCTTGGCCGACGGCACGCGTGTGGTCCCACTCCTCGCACTCCAAATAACCGTTTTCCCAAACCGAGGTCTTTGTTTTTGTGTAGCGTTTCATCACGTGGCTGCCGATGGGATGGCTGCTCACCACTTCATCAAATCCTGGGCATCCATGTATAGAGACCTTGTCGCAGGTGGCTCCGGCTCGCTTCACGGACCGCCGCCCAACCACGACAGGGCAGCGGTCAAGGACCCAAATGACTTAGATTTAGAGTCTATCTTTTTACAAGAGTTGTCGGGCTGGGCTTCTTCTTGGAACCCAGTACCTGATAAGCCCATAAAAGATCTTGCGCCCACTGACAAGGATAGGGCGACGTTTGTCCTCACCAAAGCCCAAATCGAGAGATTGAAAACTTGGGTTTCAGCTCGAGCAGTAAATGACGAAGAGTTGGGGCCGTTGCGATTGTCAGCATTCGTTGTTACATGCTCTCTCATTTGGGTTTGCATGATCCAATCAACAGAGAGCGAATTAGGGAATGGTAATGCCATTTGTAATTTTGAAGCTGATAAATCTGATGAGCCTTGCTACCTTGTATTCGCAGCAGATTGCCGGAACCGCCTCAAGGATCCAATACCTTTCACTTATTTTGGGAACTGCATCGACATTTGTGTAGTAGAATTGAAGAAGAATGAAATTCTGAAACGTCACAGTATTGTTGGAGTTGCGAGAGCTATTGGCGAGCGAGTGAAGGAGTTTGAGAGTGAACCGTTGAAAACGGCTGACAAGTGGTTCTCAGCATGGAGGAAATGTGAAGAATCGGGACGCCGTTTAGCCATTACAGGATCGCCGAGATTAAGGGTTTACGATACAGATTTTGGGTGGGGGAAGCCCCAGAAGAGTGAGATTCTCAATGTTGACTCTTCTAGGTTAGTTTGTCTGCAGGAATCTAGAGATGGGAAAGGTGGGATTGAAGTTGGTTTGGGACTTACTAAGACTCGCATGGATCGCTTTGTCGCCATGTGGGATGCAAATTTGAAGCTCTTCTGTAGT

mRNA sequence

GATGCCAACATCGTAAATGAGTGCTGGGTTTCTCCACCCGCAGGCTCAGTTGCCACCACAAATCTCCCCTTGTCTTTCTTCGACATGCTGTGGATTCCTAACCCCCCGATGCGACGCTTTTTCTTCTACGACTTCCCCCACTCCACACACCACTTCATGGACGCCATTTTTCCCACTCTCACACGCTCCCTTTCCCTCGTTCTCCAACACTTTTTTCCTCTCGCCGGAAATCTCATCTTCCCTTCTCAACCCCACACCCACAAGCCCCACATTCTCTACACAACGGCCCACGACTCGGTTCCCCTTACAGTGGCCGAGTCACCTCAAACTCAAAGTGGGTGCGACGTTTTCAACCGCCTCATCGCAGATTACCCCCGCCACGCCAAACACTTGTACCCCTTTGTTCCGGGCTTGCCCAAGCCACGTGTCTTGGCCGACGGCACGCGTGTGGTCCCACTCCTCGCACTCCAAATAACCGTTTTCCCAAACCGAGGTCTTTGTTTTTGTGTAGCGTTTCATCACGTGGCTGCCGATGGGATGGCTGCTCACCACTTCATCAAATCCTGGGCATCCATGTATAGAGACCTTGTCGCAGGTGGCTCCGGCTCGCTTCACGGACCGCCGCCCAACCACGACAGGGCAGCGGTCAAGGACCCAAATGACTTAGATTTAGAGTCTATCTTTTTACAAGAGTTGTCGGGCTGGGCTTCTTCTTGGAACCCAGTACCTGATAAGCCCATAAAAGATCTTGCGCCCACTGACAAGGATAGGGCGACGTTTGTCCTCACCAAAGCCCAAATCGAGAGATTGAAAACTTGGGTTTCAGCTCGAGCAGTAAATGACGAAGAGTTGGGGCCGTTGCGATTGTCAGCATTCGTTGTTACATGCTCTCTCATTTGGGTTTGCATGATCCAATCAACAGAGAGCGAATTAGGGAATGGTAATGCCATTTGTAATTTTGAAGCTGATAAATCTGATGAGCCTTGCTACCTTGTATTCGCAGCAGATTGCCGGAACCGCCTCAAGGATCCAATACCTTTCACTTATTTTGGGAACTGCATCGACATTTGTGTAGTAGAATTGAAGAAGAATGAAATTCTGAAACGTCACAGTATTGTTGGAGTTGCGAGAGCTATTGGCGAGCGAGTGAAGGAGTTTGAGAGTGAACCGTTGAAAACGGCTGACAAGTGGTTCTCAGCATGGAGGAAATGTGAAGAATCGGGACGCCGTTTAGCCATTACAGGATCGCCGAGATTAAGGGTTTACGATACAGATTTTGGGTGGGGGAAGCCCCAGAAGAGTGAGATTCTCAATGTTGACTCTTCTAGGTTAGTTTGTCTGCAGGAATCTAGAGATGGGAAAGGTGGGATTGAAGTTGGTTTGGGACTTACTAAGACTCGCATGGATCGCTTTGTCGCCATGTGGGATGCAAATTTGAAGCTCTTCTGTAGT

Coding sequence (CDS)

GATGCCAACATCGTAAATGAGTGCTGGGTTTCTCCACCCGCAGGCTCAGTTGCCACCACAAATCTCCCCTTGTCTTTCTTCGACATGCTGTGGATTCCTAACCCCCCGATGCGACGCTTTTTCTTCTACGACTTCCCCCACTCCACACACCACTTCATGGACGCCATTTTTCCCACTCTCACACGCTCCCTTTCCCTCGTTCTCCAACACTTTTTTCCTCTCGCCGGAAATCTCATCTTCCCTTCTCAACCCCACACCCACAAGCCCCACATTCTCTACACAACGGCCCACGACTCGGTTCCCCTTACAGTGGCCGAGTCACCTCAAACTCAAAGTGGGTGCGACGTTTTCAACCGCCTCATCGCAGATTACCCCCGCCACGCCAAACACTTGTACCCCTTTGTTCCGGGCTTGCCCAAGCCACGTGTCTTGGCCGACGGCACGCGTGTGGTCCCACTCCTCGCACTCCAAATAACCGTTTTCCCAAACCGAGGTCTTTGTTTTTGTGTAGCGTTTCATCACGTGGCTGCCGATGGGATGGCTGCTCACCACTTCATCAAATCCTGGGCATCCATGTATAGAGACCTTGTCGCAGGTGGCTCCGGCTCGCTTCACGGACCGCCGCCCAACCACGACAGGGCAGCGGTCAAGGACCCAAATGACTTAGATTTAGAGTCTATCTTTTTACAAGAGTTGTCGGGCTGGGCTTCTTCTTGGAACCCAGTACCTGATAAGCCCATAAAAGATCTTGCGCCCACTGACAAGGATAGGGCGACGTTTGTCCTCACCAAAGCCCAAATCGAGAGATTGAAAACTTGGGTTTCAGCTCGAGCAGTAAATGACGAAGAGTTGGGGCCGTTGCGATTGTCAGCATTCGTTGTTACATGCTCTCTCATTTGGGTTTGCATGATCCAATCAACAGAGAGCGAATTAGGGAATGGTAATGCCATTTGTAATTTTGAAGCTGATAAATCTGATGAGCCTTGCTACCTTGTATTCGCAGCAGATTGCCGGAACCGCCTCAAGGATCCAATACCTTTCACTTATTTTGGGAACTGCATCGACATTTGTGTAGTAGAATTGAAGAAGAATGAAATTCTGAAACGTCACAGTATTGTTGGAGTTGCGAGAGCTATTGGCGAGCGAGTGAAGGAGTTTGAGAGTGAACCGTTGAAAACGGCTGACAAGTGGTTCTCAGCATGGAGGAAATGTGAAGAATCGGGACGCCGTTTAGCCATTACAGGATCGCCGAGATTAAGGGTTTACGATACAGATTTTGGGTGGGGGAAGCCCCAGAAGAGTGAGATTCTCAATGTTGACTCTTCTAGGTTAGTTTGTCTGCAGGAATCTAGAGATGGGAAAGGTGGGATTGAAGTTGGTTTGGGACTTACTAAGACTCGCATGGATCGCTTTGTCGCCATGTGGGATGCAAATTTGAAGCTCTTCTGTAGT

Protein sequence

DANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLTRSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLIADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMAAHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNPVPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVELKKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANLKLFCS
Homology
BLAST of MC02g0509 vs. ExPASy Swiss-Prot
Match: Q9LR73 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=3AT2 PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.7e-85
Identity = 177/486 (36.42%), Postives = 269/486 (55.35%), Query Frame = 0

Query: 3   NIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLTR 62
           NI+  C +SPP G+VA+T LPL+FFD  W+  P     FF+ + +ST  F+    P L  
Sbjct: 9   NIIETCHISPPKGTVASTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKH 68

Query: 63  SLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLIA 122
           SLS+ LQHFFP AG LI P +P    P++ Y    DS+  TVAES +T      F++L +
Sbjct: 69  SLSITLQHFFPYAGKLIIPPRP--DPPYLHYNAGEDSLVFTVAESTETD-----FDQLKS 128

Query: 123 DYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMAA 182
           D P+    L+  +P LP P V  +G ++ P++A+Q+T+FP  G+C   +  HV ADG+  
Sbjct: 129 DSPKDISVLHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTF 188

Query: 183 HHFIKSWASMYRDLVAGGSGSLHGPPPNHD-RAAVKDPNDLDLESIFLQELSGWASSWNP 242
            HF+K W S+ +      +  L    P H  R  +KDP ++    +           W+ 
Sbjct: 189 SHFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHL--------ERFWSQ 248

Query: 243 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 302
              K    + P +  RATF L++ QI+ LK+WV+ ++ N        +S FVVT + IWV
Sbjct: 249 NSAKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSP-----VSTFVVTLAFIWV 308

Query: 303 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLK--DPIPFTYFGNCIDICVV 362
            +I++          + + E +  DE  +L+   DCRNRLK   PIP TYFGNC+   +V
Sbjct: 309 SLIKT---------LVQDSETEAKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIV 368

Query: 363 ELKKNEILKRHSIVGVARAIGERVKE-FESEPLKTADKWFSAWRKCEESGRRLAITGSPR 422
            +KK+++L    ++  + AI  R+K+   S+ LKTA +W    RK   S    +I G+P+
Sbjct: 369 SVKKHDLLGEKCVMAASDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPK 428

Query: 423 LRVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDAN 482
           L +YD DFG GKP K EI+++++   +   ESRDG  G+E+G+ L K +MD F ++    
Sbjct: 429 LGLYDMDFGLGKPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSLLQKG 465

Query: 483 LKLFCS 485
           +K F +
Sbjct: 489 IKKFAT 465

BLAST of MC02g0509 vs. ExPASy Swiss-Prot
Match: Q9ZWB4 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=3AT1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.2e-83
Identity = 178/485 (36.70%), Postives = 268/485 (55.26%), Query Frame = 0

Query: 4   IVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLTRS 63
           I+  C +SPP G+V +T LPL+FFD  W+  P     FF+ + +ST  F+    P L  S
Sbjct: 10  IIETCHISPPKGTVPSTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKHS 69

Query: 64  LSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLIAD 123
           LS+ LQHFFP AG LI P +P    P++ Y    DS+  TVAES +T      F++L +D
Sbjct: 70  LSITLQHFFPYAGKLIIPPRP--DPPYLHYNDGQDSLVFTVAESTETD-----FDQLKSD 129

Query: 124 YPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMAAH 183
            P+    L+  +P LP P V  +G ++ P++A+Q+T+FP  G+C   +  HV ADG+   
Sbjct: 130 SPKDISVLHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFS 189

Query: 184 HFIKSWASMYRDLVAGGSGSLHGPPPNHD-RAAVKDPNDLDLESIFLQELSGWASSWNPV 243
           HF+K W S+ +      +  L    P H  R  +KDP ++    +           W+  
Sbjct: 190 HFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHL--------ERFWSQN 249

Query: 244 PDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWVC 303
             K    + P +  RATF L++ QI+ LK+WV+ ++ N        +S FVVT + IWV 
Sbjct: 250 SAKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSP-----VSTFVVTLAFIWVS 309

Query: 304 MIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLK--DPIPFTYFGNCIDICVVE 363
           +I++    L   +     E DK DE  +L+   DCRNRLK   PIP TYFGNC+   +V 
Sbjct: 310 LIKT----LVQDSETKANEEDK-DEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVS 369

Query: 364 LKKNEILKRHSIVGVARAIGERVKE-FESEPLKTADKWFSAWRKCEESGRRLAITGSPRL 423
           +KK+++L    ++  + AI  R+K+   S+ LKTA +W    RK   S    +I G+P+L
Sbjct: 370 VKKHDLLGEKCVLAASDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKL 429

Query: 424 RVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANL 483
            +YD DFG GKP K EI+++++   +   ESRDG  G+E+G+ L K +MD F ++    +
Sbjct: 430 GLYDMDFGLGKPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGI 469

Query: 484 KLFCS 485
           K F +
Sbjct: 490 KKFAT 469

BLAST of MC02g0509 vs. ExPASy Swiss-Prot
Match: Q9MBC1 (Anthocyanidin 3-O-glucoside 6''-O-acyltransferase (Fragment) OS=Perilla frutescens OX=48386 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.3e-69
Identity = 160/479 (33.40%), Postives = 250/479 (52.19%), Query Frame = 0

Query: 4   IVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLTRS 63
           ++  C V PP  SVA  ++PL+FFDM W+   PM +  FY+FP S  HF ++I P L +S
Sbjct: 1   VIETCRVGPPPDSVAEQSVPLTFFDMTWLHFHPMLQLLFYEFPCSKQHFSESIVPKLKQS 60

Query: 64  LSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLIAD 123
           LS  L HFFPL+ NLI+PS P    P   Y +  DSV  T+AES       D F+ L+ +
Sbjct: 61  LSKTLIHFFPLSCNLIYPSSPE-KMPEFRYLSG-DSVSFTIAES------SDDFDDLVGN 120

Query: 124 YPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMAAH 183
            P     LY FVP LP P V     ++  + A+Q+T+FP RG+   +A HH  +D  +  
Sbjct: 121 RPESPVRLYNFVPKLP-PIVEESDRKLFQVFAVQVTLFPGRGVGIGIATHHTVSDAPSFL 180

Query: 184 HFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNPVP 243
            FI +W+SM + +            P  DR+ +K P   D  SI+      W ++    P
Sbjct: 181 AFITAWSSMSKHIENEDEDEEFKSLPVFDRSVIKYPTKFD--SIY------WRNALK-FP 240

Query: 244 DKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWVCM 303
            +      PTD+ R TFV T+++I++LK W+ +R  +      + LS+FV   + +W  +
Sbjct: 241 LQSRHPSLPTDRIRTTFVFTQSKIKKLKGWIQSRVPS-----LVHLSSFVAIAAYMWAGI 300

Query: 304 IQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVELKK 363
            +S  +           + D+ +E  + +   D R RL  P+P  YFGNC+   +  +++
Sbjct: 301 TKSFTA-----------DEDQDNEDAFFLIPVDLRPRLDPPVPENYFGNCLSYALPRMRR 360

Query: 364 NEILKRHSIVGVARAIGERVKE--FESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRV 423
            E++    +   A  I   +K+   +   L+T +KW    RK  +     ++ GS +L +
Sbjct: 361 RELVGEKGVFLAAEVIAAEIKKRINDKRILETVEKWSPEIRKALQKS-YFSVAGSSKLDL 420

Query: 424 YDTDFGWGKPQKSEILNVDSSR-LVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANL 480
           Y  DFGWGK +K EIL++D  +  + L ++RD +GG+EV L L K +MD F A +   +
Sbjct: 421 YGADFGWGKARKQEILSIDGEKYAMTLCKARDFEGGLEVCLSLPKDKMDAFAAYFSLGI 444

BLAST of MC02g0509 vs. ExPASy Swiss-Prot
Match: Q589Y0 (Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.8e-68
Identity = 163/484 (33.68%), Postives = 251/484 (51.86%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A+++ +C V P  GS     LPL++FD +W+    MRR  FY  P S   F+  I PTL 
Sbjct: 2   ASVIEQCQVVPSPGSATELTLPLTYFDHVWLAFHRMRRILFYKLPISRPDFVQTIIPTLK 61

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
            SLSL L+++ PLAGN+  P Q  +  P + Y T  +SV +  +ES         FN LI
Sbjct: 62  DSLSLTLKYYLPLAGNVACP-QDWSGYPELRYVTG-NSVSVIFSESDMD------FNYLI 121

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
             +PR+ K  Y FVP L +P+  A G ++ P+LA+Q+T+FPN G+      HHVA DG  
Sbjct: 122 GYHPRNTKDFYHFVPQLAEPKD-APGVQLAPVLAIQVTLFPNHGISIGFTNHHVAGDGAT 181

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPP--PNHDRAAVKDPNDLDLESIFLQELSGWASSW 241
              F+++WA + +    GG          P +DR+ +KDPN + +            S W
Sbjct: 182 IVKFVRAWALLNK---FGGDEQFLANEFIPFYDRSVIKDPNGVGM------------SIW 241

Query: 242 NPVPD-----KPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVV 301
           N +       K    + P DK R TF++T+  I +LK  V  R           +++F V
Sbjct: 242 NEMKKYKHMMKMSDVVTPPDKVRGTFIITRHDIGKLKNLVLTRRPK-----LTHVTSFTV 301

Query: 302 TCSLIWVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCI 361
           TC+ +W C+I+S         A    E D++    +   AADCR +   P+P +YFGN +
Sbjct: 302 TCAYVWTCIIKS--------EAATGEEIDENGME-FFGCAADCRAQFNPPLPPSYFGNAL 361

Query: 362 DICVVELKKNEILKRHSIVGVARAIGERV-KEFESEPLKTADKWFSAWRKCEESGRRLAI 421
              V   ++ ++  +         IGE + K  + E    +  WF  + K  ++ R L++
Sbjct: 362 VGYVARTRQVDLAGKEGFTIAVELIGEAIRKRMKDEEWILSGSWFKEYDKV-DAKRSLSV 421

Query: 422 TGSPRLRVYDTDFGWGKPQKSEILNVDSSR--LVCLQESRDGKGGIEVGLGLTKTRMDRF 476
            GSP+L +Y  DFGWG+P+K E +++D+     + L +S+D  G +E+GL L+KTRM+ F
Sbjct: 422 AGSPKLDLYAADFGWGRPEKLEFVSIDNDDGISMSLSKSKDSDGDLEIGLSLSKTRMNAF 446

BLAST of MC02g0509 vs. ExPASy Swiss-Prot
Match: Q9ZWR8 (Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.2e-66
Identity = 162/492 (32.93%), Postives = 253/492 (51.42%), Query Frame = 0

Query: 4   IVNECWVSPPAGSV-ATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLTR 63
           ++ +C V+PP+ +     +LP++FFD+ W+    M+   FYDFP+   HF+D + P L  
Sbjct: 9   VLEKCQVTPPSDTTDVELSLPVTFFDIPWLHLNKMQSLLFYDFPYPRTHFLDTVIPNLKA 68

Query: 64  SLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAH-DSVPLTVAESPQTQSGCDVFNRLI 123
           SLSL L+H+ PL+GNL+ P +     P   Y+    DS+ L VAES Q       F+ L 
Sbjct: 69  SLSLTLKHYVPLSGNLLMPIK-SGEMPKFQYSRDEGDSITLIVAESDQD------FDYLK 128

Query: 124 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 183
                 +  L+     +P+        +V+PL+A+Q+TVFPNRG+   +  HH  AD  +
Sbjct: 129 GHQLVDSNDLHGLFYVMPRVIRTMQDYKVIPLVAVQVTVFPNRGIAVALTAHHSIADAKS 188

Query: 184 AHHFIKSWASMYR-----DLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWA 243
              FI +WA + +     DL++          P+ DR+ +KD     LE  F  E+    
Sbjct: 189 FVMFINAWAYINKFGKDADLLSANL------LPSFDRSIIKDL--YGLEETFWNEMQDVL 248

Query: 244 SSWNPVPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGP-LRLSAFVVT 303
             ++    KP +     +K RAT+VL+ A+I++LK     + +N     P +R++ F +T
Sbjct: 249 EMFSRFGSKPPR----FNKVRATYVLSLAEIQKLKN----KVLNLRGSEPTIRVTTFTMT 308

Query: 304 CSLIWVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCID 363
           C  +W CM++S +      + +    ++  +E  Y  F ADCR  L  P P  YFGNC+ 
Sbjct: 309 CGYVWTCMVKSKD------DVVSEESSNDENELEYFSFTADCRGLLTPPCPPNYFGNCLA 368

Query: 364 ICVVELKKNEILKRHSIVGVARAIGERVKE---FESEPLKTADKWFSAWRKCEESGRRLA 423
            CV +    E++    ++    AIGE +++    E   L  A  W S       S R L 
Sbjct: 369 SCVAKATHKELVGDKGLLVAVAAIGEAIEKRLHNEKGVLADAKTWLSESNGI-PSKRFLG 428

Query: 424 ITGSPRLRVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFV 483
           ITGSP+   Y  DFGWGKP K +I +VD + L+ + +SRD + G+E+G+ L K  MD F 
Sbjct: 429 ITGSPKFDSYGVDFGWGKPAKFDITSVDYAELIYVIQSRDFEKGVEIGVSLPKIHMDAFA 467

Query: 484 AMWDANLKLFCS 485
            +++     FCS
Sbjct: 489 KIFEEG---FCS 467

BLAST of MC02g0509 vs. NCBI nr
Match: XP_022140373.1 (LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Momordica charantia])

HSP 1 Score: 656 bits (1693), Expect = 1.19e-232
Identity = 332/485 (68.45%), Postives = 376/485 (77.53%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N C +SPPAGSV TT+LPLSF DMLWI   PMRR FFY FPHSTHHF+ AIFPTL 
Sbjct: 10  AKVLNHCRISPPAGSVPTTHLPLSFLDMLWIVCRPMRRLFFYHFPHSTHHFIHAIFPTLK 69

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
           RSLSL LQHFFPLAGN I P  PH  +PHIL  T HDSVPLTVAES    SGCD F+RLI
Sbjct: 70  RSLSLALQHFFPLAGNFILPPPPH--RPHIL-CTPHDSVPLTVAES----SGCD-FDRLI 129

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           AD+PR AKH YP VP LP  R   DG RVVP+ ALQITVFPN+GLC  +AFHHV ADGMA
Sbjct: 130 ADHPRPAKHFYPLVPDLPNSRASPDGARVVPVFALQITVFPNQGLCISIAFHHVVADGMA 189

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
           AH F+KS AS++R+   GGSGSL GP P HDRAAVKDPN L  ESIFLQELS W S WNP
Sbjct: 190 AHDFLKSXASIHRN--GGGSGSLDGPLPYHDRAAVKDPNGL--ESIFLQELSYWDSLWNP 249

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVND--EELGPLRLSAFVVTCSLI 301
           VPDKPI DLAP DK RATFV+ K QIERLKTW+S +A +D  EEL PLRLSA +VTCSLI
Sbjct: 250 VPDKPIIDLAPADKVRATFVINKTQIERLKTWISNQAAHDDNEELKPLRLSALIVTCSLI 309

Query: 302 WVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVV 361
           W C+I+STE E    +       D+ DE C+ V  ADCRNRLKDP+P TYFGNC+D+  V
Sbjct: 310 WGCLIKSTEGEFAQNDL-----PDELDELCFFVLLADCRNRLKDPLPPTYFGNCLDMGAV 369

Query: 362 ELKKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRL 421
            LKK+E+LK +S V  A+AIG+RV+EFESEPLKTADKWFS WR C ES   L I GSP+L
Sbjct: 370 VLKKSELLKSNSSVVAAKAIGKRVEEFESEPLKTADKWFSEWR-CLESKHLLTIAGSPKL 429

Query: 422 RVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANL 481
           RVYDTDFGWGKP+KSE+L+VDSS  + LQE RD KGGIEVGLGLTKT MDRF+A+W+ NL
Sbjct: 430 RVYDTDFGWGKPKKSEVLHVDSSLAISLQECRDEKGGIEVGLGLTKTHMDRFIAIWEENL 476

Query: 482 KLFCS 484
            + C+
Sbjct: 490 NVLCT 476

BLAST of MC02g0509 vs. NCBI nr
Match: XP_022971001.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 621 bits (1601), Expect = 9.23e-219
Identity = 303/482 (62.86%), Postives = 365/482 (75.73%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N  W+SPP GSV TT LPLSF D LW    PMRR FF+ FPHST+HF+D++FPTL 
Sbjct: 8   AKLLNLYWISPPPGSVPTTRLPLSFLDFLWTICSPMRRLFFFQFPHSTNHFIDSVFPTLK 67

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
            SLSL LQ FFPLAGNLI P  PH  KPHILYT  HDSVPLTVAES    +G D FN LI
Sbjct: 68  HSLSLSLQQFFPLAGNLILPPPPH--KPHILYTP-HDSVPLTVAES----TGGD-FNTLI 127

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           AD PR+AKH YPFVP LPK R+L DG+R +P  A+QIT+FPN+GLC C+AFHH  ADGMA
Sbjct: 128 ADSPRNAKHFYPFVPDLPKSRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHAVADGMA 187

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
            HHF+KSWAS+YR+   G S S  GP P HDRAAVKDPN L  ESIFL ELS W S WNP
Sbjct: 188 VHHFLKSWASIYRN--GGDSVSFEGPLPCHDRAAVKDPNGL--ESIFLHELSSWDSLWNP 247

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 301
           VP+KPI D AP DK R T VLT+  IE+LKTWVSA++ +D+EL PLRLS+F+VTCSL+W 
Sbjct: 248 VPEKPIIDQAPVDKVRTTLVLTRPIIEKLKTWVSAQSNSDKELKPLRLSSFIVTCSLVWG 307

Query: 302 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVEL 361
           C+I+ST++E           ++  DE CY V  ADCRNRLKD IP TYFGNC+D+  V L
Sbjct: 308 CLIKSTQNEASG--------SEDEDELCYFVLVADCRNRLKDQIPPTYFGNCLDVGAVVL 367

Query: 362 KKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRV 421
           KK+E+LKR+SIV VA+AIG RV+EFESEP K A++WFS WR C ++   L++ GSP+ R 
Sbjct: 368 KKSELLKRNSIVVVAKAIGNRVEEFESEPFKMAEEWFSLWRACGKAKHFLSVAGSPKSRS 427

Query: 422 YDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANLKL 481
           YDTDFGWG+P+KSE+L+VDSS  + L ESRD +GGIE+G+GL KT+MDR + +W+ NLK 
Sbjct: 428 YDTDFGWGRPKKSEVLHVDSSIAISLHESRDEEGGIEIGMGLHKTQMDRLMTIWEQNLKG 469

Query: 482 FC 483
            C
Sbjct: 488 LC 469

BLAST of MC02g0509 vs. NCBI nr
Match: XP_022971000.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 615 bits (1585), Expect = 2.49e-216
Identity = 301/483 (62.32%), Postives = 366/483 (75.78%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N   +SPPAGSV TT LP+SF D +W+   PMRR FF+ FPHST+HFMD++FPTL 
Sbjct: 8   AKLLNLYRISPPAGSVPTTLLPISFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPTLK 67

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
            SLSL LQHFFPLAGNLI P QP   KPHILYT  HDSVPLTV ES    +G D FN LI
Sbjct: 68  SSLSLSLQHFFPLAGNLILPPQPQ--KPHILYTP-HDSVPLTVTES----TGGD-FNTLI 127

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           ADYPR AKH YPFVP +P+ R+L DG+R +P  A+QIT+FPN+GLC C+AFHHV ADG+A
Sbjct: 128 ADYPRDAKHFYPFVPDMPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHVVADGIA 187

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
            HHFIKSWAS+ R+   G S S  GP P H+RAA+KD   L  ESIFLQELS W S WNP
Sbjct: 188 IHHFIKSWASISRN--GGTSDSFEGPLPCHNRAAIKDVKGL--ESIFLQELSSWDSLWNP 247

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 301
           VP KPI D AP D+ R T VLT+  IE+LKTWVSA + NDEEL PLRLS+F+VTCSL+W 
Sbjct: 248 VPYKPIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEELKPLRLSSFIVTCSLVWG 307

Query: 302 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVEL 361
           C+I+ST+SE+         +++  DE C+  F ADCRNRLKDP+P TYFGNC+D+ VVEL
Sbjct: 308 CLIKSTQSEVS--------DSEDEDELCHFGFLADCRNRLKDPLPPTYFGNCLDVGVVEL 367

Query: 362 KKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRV 421
           KK+E++KR+S V  A+AIG RV+EFE+EPLK A+KWFS W+   +    + I GSP+LR 
Sbjct: 368 KKSELVKRNSTVMAAKAIGNRVEEFENEPLKVAEKWFSLWKAYSKDKHFVTIAGSPKLRA 427

Query: 422 YDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANLKL 481
           YDTDFGWG+  KSE+L+VDSS  + LQ+SRD +GGIE+GLGL  TRMDRF+A+W+ NLK 
Sbjct: 428 YDTDFGWGRAMKSEVLHVDSSESISLQDSRDEEGGIEIGLGLHNTRMDRFMAIWEQNLKD 470

Query: 482 FCS 484
            CS
Sbjct: 488 LCS 470

BLAST of MC02g0509 vs. NCBI nr
Match: XP_023533558.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615 bits (1586), Expect = 6.85e-216
Identity = 301/483 (62.32%), Postives = 367/483 (75.98%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N   +SPPAGSV TT LPLSF D +W+   PMRR FF+ FPHST+HFMD++FPTL 
Sbjct: 46  AKLLNLYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPTLK 105

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
           RSLSL LQHFFPLAGNLI P QPH  KPHILYT  HDSVPLTVAES    +G D F  LI
Sbjct: 106 RSLSLSLQHFFPLAGNLILPPQPH--KPHILYTP-HDSVPLTVAES----TGGD-FTTLI 165

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           ADYPR AKH YPFVP +P+ R+L DG+R +P  A+QIT+FPN+GLC C+AF HV ADG+A
Sbjct: 166 ADYPRDAKHFYPFVPDMPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFQHVVADGIA 225

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
            HHFIKSWAS+ R+   G S S  GP P H+RAA+KD   L  ESIFLQELS W S WNP
Sbjct: 226 IHHFIKSWASISRN--GGTSDSFEGPLPCHNRAAIKDVKGL--ESIFLQELSSWDSLWNP 285

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 301
           VP KPI D AP D+ R T VLT+  IE+LKTWVSA + NDEEL PLRLS+F+VTCSL+W 
Sbjct: 286 VPYKPIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEELKPLRLSSFIVTCSLVWG 345

Query: 302 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVEL 361
           C+I+ST+SE          +++  DE CY  F ADCRNR+KDP+P TYFGNC+D+ VVEL
Sbjct: 346 CLIKSTQSEAS--------DSEDEDELCYFGFLADCRNRVKDPLPPTYFGNCLDVGVVEL 405

Query: 362 KKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRV 421
           KK+E++KR+SIV  A+AIG RV+EFE+EPLK A+KWFS W+   +    + I GSP+L+ 
Sbjct: 406 KKSELVKRNSIVMAAKAIGNRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKA 465

Query: 422 YDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANLKL 481
           YDTDFGWG+  KSE+L+VD+S  + LQ+SRD +GGIE+GLGL  T+MDRF+A+W+ NLK 
Sbjct: 466 YDTDFGWGRAMKSEVLHVDNSESISLQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNLKD 508

Query: 482 FCS 484
            CS
Sbjct: 526 LCS 508

BLAST of MC02g0509 vs. NCBI nr
Match: KAG6604829.1 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612 bits (1578), Expect = 2.89e-215
Identity = 299/482 (62.03%), Postives = 361/482 (74.90%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N  W+SPPAGSV TT LPLSF D LW    PMRR FF+ FPHST+HF+D++FPTL 
Sbjct: 8   AKLLNLYWISPPAGSVPTTRLPLSFLDFLWTICSPMRRLFFFQFPHSTNHFIDSVFPTLK 67

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
             LSL LQ FFPLAGNLI P  PH  KPHILYT  HDSVPLTV ES    +G D FN LI
Sbjct: 68  HCLSLSLQQFFPLAGNLILPPPPH--KPHILYTP-HDSVPLTVTES----TGGD-FNTLI 127

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           AD PR AKH YPFVP LP+ R+L DG+R +P  A+QIT+FPN+GLC C+AFHH  ADGMA
Sbjct: 128 ADSPRDAKHFYPFVPDLPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHAVADGMA 187

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
            HHF+KSWAS+YR+   G S S  GP P HDRAAVKDPN L  ESIF +ELS W S WNP
Sbjct: 188 VHHFLKSWASIYRN--GGDSVSFEGPLPCHDRAAVKDPNGL--ESIFSRELSSWDSLWNP 247

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 301
           VP KPI D AP DK R T VLT+  IE+LKTWVSA + +D+EL PLRLS+F+VTCSL+W 
Sbjct: 248 VPYKPIIDQAPIDKVRTTLVLTRPIIEKLKTWVSAESNSDKELKPLRLSSFIVTCSLVWG 307

Query: 302 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVEL 361
           C+I+ST+SE           ++  DE CY V  ADCRNRLKD IP TYFGNC+D+  V L
Sbjct: 308 CLIKSTQSEASG--------SEDEDELCYFVLVADCRNRLKDQIPPTYFGNCLDVGAVVL 367

Query: 362 KKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRV 421
           +K+E+LKR+S   VA+AIG+RV+EFESEP K A++WFS WR C ++   L++ GSP+ R 
Sbjct: 368 RKSELLKRNSTAIVAKAIGKRVEEFESEPFKMAEEWFSLWRACGKAKHFLSVAGSPKSRT 427

Query: 422 YDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANLKL 481
           YDTDFGWG+P+KSE+L+VDSS  + L ESRD +GGIE+G+GL KT+MDRF+ +W+ NLK 
Sbjct: 428 YDTDFGWGRPKKSEVLHVDSSIAISLHESRDEEGGIEIGMGLHKTQMDRFMTIWEQNLKG 469

Query: 482 FC 483
            C
Sbjct: 488 LC 469

BLAST of MC02g0509 vs. ExPASy TrEMBL
Match: A0A6J1CHV4 (LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111011063 PE=3 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 5.75e-233
Identity = 332/485 (68.45%), Postives = 376/485 (77.53%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N C +SPPAGSV TT+LPLSF DMLWI   PMRR FFY FPHSTHHF+ AIFPTL 
Sbjct: 10  AKVLNHCRISPPAGSVPTTHLPLSFLDMLWIVCRPMRRLFFYHFPHSTHHFIHAIFPTLK 69

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
           RSLSL LQHFFPLAGN I P  PH  +PHIL  T HDSVPLTVAES    SGCD F+RLI
Sbjct: 70  RSLSLALQHFFPLAGNFILPPPPH--RPHIL-CTPHDSVPLTVAES----SGCD-FDRLI 129

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           AD+PR AKH YP VP LP  R   DG RVVP+ ALQITVFPN+GLC  +AFHHV ADGMA
Sbjct: 130 ADHPRPAKHFYPLVPDLPNSRASPDGARVVPVFALQITVFPNQGLCISIAFHHVVADGMA 189

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
           AH F+KS AS++R+   GGSGSL GP P HDRAAVKDPN L  ESIFLQELS W S WNP
Sbjct: 190 AHDFLKSXASIHRN--GGGSGSLDGPLPYHDRAAVKDPNGL--ESIFLQELSYWDSLWNP 249

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVND--EELGPLRLSAFVVTCSLI 301
           VPDKPI DLAP DK RATFV+ K QIERLKTW+S +A +D  EEL PLRLSA +VTCSLI
Sbjct: 250 VPDKPIIDLAPADKVRATFVINKTQIERLKTWISNQAAHDDNEELKPLRLSALIVTCSLI 309

Query: 302 WVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVV 361
           W C+I+STE E    +       D+ DE C+ V  ADCRNRLKDP+P TYFGNC+D+  V
Sbjct: 310 WGCLIKSTEGEFAQNDL-----PDELDELCFFVLLADCRNRLKDPLPPTYFGNCLDMGAV 369

Query: 362 ELKKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRL 421
            LKK+E+LK +S V  A+AIG+RV+EFESEPLKTADKWFS WR C ES   L I GSP+L
Sbjct: 370 VLKKSELLKSNSSVVAAKAIGKRVEEFESEPLKTADKWFSEWR-CLESKHLLTIAGSPKL 429

Query: 422 RVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANL 481
           RVYDTDFGWGKP+KSE+L+VDSS  + LQE RD KGGIEVGLGLTKT MDRF+A+W+ NL
Sbjct: 430 RVYDTDFGWGKPKKSEVLHVDSSLAISLQECRDEKGGIEVGLGLTKTHMDRFIAIWEENL 476

Query: 482 KLFCS 484
            + C+
Sbjct: 490 NVLCT 476

BLAST of MC02g0509 vs. ExPASy TrEMBL
Match: A0A6J1I239 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469804 PE=3 SV=1)

HSP 1 Score: 621 bits (1601), Expect = 4.47e-219
Identity = 303/482 (62.86%), Postives = 365/482 (75.73%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N  W+SPP GSV TT LPLSF D LW    PMRR FF+ FPHST+HF+D++FPTL 
Sbjct: 8   AKLLNLYWISPPPGSVPTTRLPLSFLDFLWTICSPMRRLFFFQFPHSTNHFIDSVFPTLK 67

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
            SLSL LQ FFPLAGNLI P  PH  KPHILYT  HDSVPLTVAES    +G D FN LI
Sbjct: 68  HSLSLSLQQFFPLAGNLILPPPPH--KPHILYTP-HDSVPLTVAES----TGGD-FNTLI 127

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           AD PR+AKH YPFVP LPK R+L DG+R +P  A+QIT+FPN+GLC C+AFHH  ADGMA
Sbjct: 128 ADSPRNAKHFYPFVPDLPKSRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHAVADGMA 187

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
            HHF+KSWAS+YR+   G S S  GP P HDRAAVKDPN L  ESIFL ELS W S WNP
Sbjct: 188 VHHFLKSWASIYRN--GGDSVSFEGPLPCHDRAAVKDPNGL--ESIFLHELSSWDSLWNP 247

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 301
           VP+KPI D AP DK R T VLT+  IE+LKTWVSA++ +D+EL PLRLS+F+VTCSL+W 
Sbjct: 248 VPEKPIIDQAPVDKVRTTLVLTRPIIEKLKTWVSAQSNSDKELKPLRLSSFIVTCSLVWG 307

Query: 302 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVEL 361
           C+I+ST++E           ++  DE CY V  ADCRNRLKD IP TYFGNC+D+  V L
Sbjct: 308 CLIKSTQNEASG--------SEDEDELCYFVLVADCRNRLKDQIPPTYFGNCLDVGAVVL 367

Query: 362 KKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRV 421
           KK+E+LKR+SIV VA+AIG RV+EFESEP K A++WFS WR C ++   L++ GSP+ R 
Sbjct: 368 KKSELLKRNSIVVVAKAIGNRVEEFESEPFKMAEEWFSLWRACGKAKHFLSVAGSPKSRS 427

Query: 422 YDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANLKL 481
           YDTDFGWG+P+KSE+L+VDSS  + L ESRD +GGIE+G+GL KT+MDR + +W+ NLK 
Sbjct: 428 YDTDFGWGRPKKSEVLHVDSSIAISLHESRDEEGGIEIGMGLHKTQMDRLMTIWEQNLKG 469

Query: 482 FC 483
            C
Sbjct: 488 LC 469

BLAST of MC02g0509 vs. ExPASy TrEMBL
Match: A0A6J1I4H8 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469803 PE=3 SV=1)

HSP 1 Score: 615 bits (1585), Expect = 1.21e-216
Identity = 301/483 (62.32%), Postives = 366/483 (75.78%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N   +SPPAGSV TT LP+SF D +W+   PMRR FF+ FPHST+HFMD++FPTL 
Sbjct: 8   AKLLNLYRISPPAGSVPTTLLPISFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPTLK 67

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
            SLSL LQHFFPLAGNLI P QP   KPHILYT  HDSVPLTV ES    +G D FN LI
Sbjct: 68  SSLSLSLQHFFPLAGNLILPPQPQ--KPHILYTP-HDSVPLTVTES----TGGD-FNTLI 127

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           ADYPR AKH YPFVP +P+ R+L DG+R +P  A+QIT+FPN+GLC C+AFHHV ADG+A
Sbjct: 128 ADYPRDAKHFYPFVPDMPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHVVADGIA 187

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
            HHFIKSWAS+ R+   G S S  GP P H+RAA+KD   L  ESIFLQELS W S WNP
Sbjct: 188 IHHFIKSWASISRN--GGTSDSFEGPLPCHNRAAIKDVKGL--ESIFLQELSSWDSLWNP 247

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 301
           VP KPI D AP D+ R T VLT+  IE+LKTWVSA + NDEEL PLRLS+F+VTCSL+W 
Sbjct: 248 VPYKPIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEELKPLRLSSFIVTCSLVWG 307

Query: 302 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVEL 361
           C+I+ST+SE+         +++  DE C+  F ADCRNRLKDP+P TYFGNC+D+ VVEL
Sbjct: 308 CLIKSTQSEVS--------DSEDEDELCHFGFLADCRNRLKDPLPPTYFGNCLDVGVVEL 367

Query: 362 KKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRV 421
           KK+E++KR+S V  A+AIG RV+EFE+EPLK A+KWFS W+   +    + I GSP+LR 
Sbjct: 368 KKSELVKRNSTVMAAKAIGNRVEEFENEPLKVAEKWFSLWKAYSKDKHFVTIAGSPKLRA 427

Query: 422 YDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANLKL 481
           YDTDFGWG+  KSE+L+VDSS  + LQ+SRD +GGIE+GLGL  TRMDRF+A+W+ NLK 
Sbjct: 428 YDTDFGWGRAMKSEVLHVDSSESISLQDSRDEEGGIEIGLGLHNTRMDRFMAIWEQNLKD 470

Query: 482 FCS 484
            CS
Sbjct: 488 LCS 470

BLAST of MC02g0509 vs. ExPASy TrEMBL
Match: A0A6J1G865 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451633 PE=3 SV=1)

HSP 1 Score: 607 bits (1564), Expect = 2.09e-213
Identity = 299/485 (61.65%), Postives = 361/485 (74.43%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N  W+SPPAGSV TT LPLSF D LW    PMRR FF+ FPHST+HF+D++FPTL 
Sbjct: 8   AKLLNLYWISPPAGSVPTTRLPLSFLDFLWTICSPMRRLFFFQFPHSTNHFIDSVFPTLK 67

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
            SLSL LQ FFPLAGNLI P  PH  KPHILYT  HDSVPLTV ES    +G D FN LI
Sbjct: 68  HSLSLSLQQFFPLAGNLILPPPPH--KPHILYTP-HDSVPLTVTES----TGGD-FNTLI 127

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           AD PR AKH YPFVP LP+ R+L DG+R +P  A+QIT+FPN+GLC C+AFHH  ADGMA
Sbjct: 128 ADSPRDAKHFYPFVPDLPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHAVADGMA 187

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQ---ELSGWASS 241
            HHF+KSWAS+YR+   G S S  GP P HDRAAVKDPN L  ESIF +    LS W S 
Sbjct: 188 VHHFLKSWASIYRN--GGDSVSFEGPLPCHDRAAVKDPNGL--ESIFSRCEYSLSSWDSL 247

Query: 242 WNPVPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSL 301
           WNPVP KPI D AP DK R T VLT+  IE+LKTWVSA + +D+EL PLRLS+F+VTCSL
Sbjct: 248 WNPVPYKPIIDQAPIDKVRTTLVLTRPIIEKLKTWVSAESNSDKELKPLRLSSFIVTCSL 307

Query: 302 IWVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICV 361
           +W C+I+ST+SE           ++  DE CY V  ADCRNRLKD IP TYFGNC+D+  
Sbjct: 308 VWGCLIKSTQSEASG--------SEDEDELCYFVLVADCRNRLKDQIPPTYFGNCLDVGA 367

Query: 362 VELKKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPR 421
           V L+K+E+LKR+S   VA+AIG+RV+EFESEP K A++WFS WR C ++   L++ GSP+
Sbjct: 368 VVLRKSELLKRNSTAIVAKAIGKRVEEFESEPFKMAEEWFSLWRACGKAKHFLSVAGSPK 427

Query: 422 LRVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDAN 481
            R YDTDFGWG+P+KSE+L+VDSS  + L ESRD +GGIE+G+GL KT+MDRF+ +W+ N
Sbjct: 428 SRTYDTDFGWGRPKKSEVLHVDSSIAISLHESRDEEGGIEIGMGLHKTQMDRFMTIWEQN 472

Query: 482 LKLFC 483
           LK  C
Sbjct: 488 LKGLC 472

BLAST of MC02g0509 vs. ExPASy TrEMBL
Match: A0A6J1G6S5 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451276 PE=3 SV=1)

HSP 1 Score: 606 bits (1563), Expect = 1.03e-212
Identity = 297/483 (61.49%), Postives = 364/483 (75.36%), Query Frame = 0

Query: 2   ANIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLT 61
           A ++N   +SPPAGSV TT LPLSF D +W+   PMRR FF+ FPHST+HFMD++FPTL 
Sbjct: 46  AKLLNVYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPTLK 105

Query: 62  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 121
           RSLSL LQHFFPLAGNLI P  PH  KPHILYT  HDSVPLTV ES    +G D FN LI
Sbjct: 106 RSLSLSLQHFFPLAGNLILPPPPH--KPHILYTP-HDSVPLTVTES----TGGD-FNTLI 165

Query: 122 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 181
           ADYPR AKH YPFVP +P+  +L DG+R +P  A+QIT+FPN+GLC C+AF HV ADG+A
Sbjct: 166 ADYPRDAKHFYPFVPDMPESCLLPDGSRAIPNFAIQITIFPNQGLCICIAFQHVVADGIA 225

Query: 182 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWNP 241
            HHFIKSWAS+ R+   G S S  GP P H+RAA+KD   L  ESIFLQELS W S WNP
Sbjct: 226 IHHFIKSWASISRN--GGASDSFEGPLPCHNRAAIKDVKGL--ESIFLQELSSWDSLWNP 285

Query: 242 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 301
           VP KPI D AP D+ R T VLT+  IE+LKTWVSA + NDEE  PLRLS+F+VTCSL+W 
Sbjct: 286 VPYKPIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEESKPLRLSSFIVTCSLVWG 345

Query: 302 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGNCIDICVVEL 361
           C+I+ST+SE          ++   D+ CY  F ADCRNR+KDP+P TYFGNC+D+ VVEL
Sbjct: 346 CLIKSTQSEAS--------DSVDEDKLCYFGFLADCRNRVKDPLPPTYFGNCLDVGVVEL 405

Query: 362 KKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSPRLRV 421
           KK+E++KR+SIV  A+AIG+RV+EFE+EPLK A+KWFS W+   +    + I GSP+L+ 
Sbjct: 406 KKSELVKRNSIVMAAKAIGKRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKA 465

Query: 422 YDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANLKL 481
           YDTDFGWG+  KSE+L+VDSS  + +Q+SRD +GGIE+GLGL  T+MDRF+A+W+ NLK 
Sbjct: 466 YDTDFGWGRAMKSEVLHVDSSESISVQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNLKD 508

Query: 482 FCS 484
            CS
Sbjct: 526 LCS 508

BLAST of MC02g0509 vs. TAIR 10
Match: AT1G03495.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-86
Identity = 177/486 (36.42%), Postives = 269/486 (55.35%), Query Frame = 0

Query: 3   NIVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLTR 62
           NI+  C +SPP G+VA+T LPL+FFD  W+  P     FF+ + +ST  F+    P L  
Sbjct: 9   NIIETCHISPPKGTVASTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKH 68

Query: 63  SLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLIA 122
           SLS+ LQHFFP AG LI P +P    P++ Y    DS+  TVAES +T      F++L +
Sbjct: 69  SLSITLQHFFPYAGKLIIPPRP--DPPYLHYNAGEDSLVFTVAESTETD-----FDQLKS 128

Query: 123 DYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMAA 182
           D P+    L+  +P LP P V  +G ++ P++A+Q+T+FP  G+C   +  HV ADG+  
Sbjct: 129 DSPKDISVLHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTF 188

Query: 183 HHFIKSWASMYRDLVAGGSGSLHGPPPNHD-RAAVKDPNDLDLESIFLQELSGWASSWNP 242
            HF+K W S+ +      +  L    P H  R  +KDP ++    +           W+ 
Sbjct: 189 SHFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHL--------ERFWSQ 248

Query: 243 VPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWV 302
              K    + P +  RATF L++ QI+ LK+WV+ ++ N        +S FVVT + IWV
Sbjct: 249 NSAKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSP-----VSTFVVTLAFIWV 308

Query: 303 CMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLK--DPIPFTYFGNCIDICVV 362
            +I++          + + E +  DE  +L+   DCRNRLK   PIP TYFGNC+   +V
Sbjct: 309 SLIKT---------LVQDSETEAKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIV 368

Query: 363 ELKKNEILKRHSIVGVARAIGERVKE-FESEPLKTADKWFSAWRKCEESGRRLAITGSPR 422
            +KK+++L    ++  + AI  R+K+   S+ LKTA +W    RK   S    +I G+P+
Sbjct: 369 SVKKHDLLGEKCVMAASDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPK 428

Query: 423 LRVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDAN 482
           L +YD DFG GKP K EI+++++   +   ESRDG  G+E+G+ L K +MD F ++    
Sbjct: 429 LGLYDMDFGLGKPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSLLQKG 465

Query: 483 LKLFCS 485
           +K F +
Sbjct: 489 IKKFAT 465

BLAST of MC02g0509 vs. TAIR 10
Match: AT1G03940.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 312.0 bits (798), Expect = 8.4e-85
Identity = 178/485 (36.70%), Postives = 268/485 (55.26%), Query Frame = 0

Query: 4   IVNECWVSPPAGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDAIFPTLTRS 63
           I+  C +SPP G+V +T LPL+FFD  W+  P     FF+ + +ST  F+    P L  S
Sbjct: 10  IIETCHISPPKGTVPSTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKHS 69

Query: 64  LSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLIAD 123
           LS+ LQHFFP AG LI P +P    P++ Y    DS+  TVAES +T      F++L +D
Sbjct: 70  LSITLQHFFPYAGKLIIPPRP--DPPYLHYNDGQDSLVFTVAESTETD-----FDQLKSD 129

Query: 124 YPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMAAH 183
            P+    L+  +P LP P V  +G ++ P++A+Q+T+FP  G+C   +  HV ADG+   
Sbjct: 130 SPKDISVLHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFS 189

Query: 184 HFIKSWASMYRDLVAGGSGSLHGPPPNHD-RAAVKDPNDLDLESIFLQELSGWASSWNPV 243
           HF+K W S+ +      +  L    P H  R  +KDP ++    +           W+  
Sbjct: 190 HFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHL--------ERFWSQN 249

Query: 244 PDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLIWVC 303
             K    + P +  RATF L++ QI+ LK+WV+ ++ N        +S FVVT + IWV 
Sbjct: 250 SAKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSP-----VSTFVVTLAFIWVS 309

Query: 304 MIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLK--DPIPFTYFGNCIDICVVE 363
           +I++    L   +     E DK DE  +L+   DCRNRLK   PIP TYFGNC+   +V 
Sbjct: 310 LIKT----LVQDSETKANEEDK-DEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVS 369

Query: 364 LKKNEILKRHSIVGVARAIGERVKE-FESEPLKTADKWFSAWRKCEESGRRLAITGSPRL 423
           +KK+++L    ++  + AI  R+K+   S+ LKTA +W    RK   S    +I G+P+L
Sbjct: 370 VKKHDLLGEKCVLAASDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKL 429

Query: 424 RVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDANL 483
            +YD DFG GKP K EI+++++   +   ESRDG  G+E+G+ L K +MD F ++    +
Sbjct: 430 GLYDMDFGLGKPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGI 469

Query: 484 KLFCS 485
           K F +
Sbjct: 490 KKFAT 469

BLAST of MC02g0509 vs. TAIR 10
Match: AT5G39090.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 237.7 bits (605), Expect = 2.0e-62
Identity = 157/483 (32.51%), Postives = 238/483 (49.28%), Query Frame = 0

Query: 3   NIVNECWVSPP-AGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDA-IFPTL 62
           N ++   V+P  + S A+  LPL+FFD+LW+ +  + R  FY          D+ I P L
Sbjct: 6   NFIHVSRVTPSNSNSSASLTLPLTFFDLLWLKHKAVERVIFYKLTDVNRSLFDSVIVPNL 65

Query: 63  TRSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRL 122
             SLS  L H+ PLAG++I+  +PH  KP I+Y T +D+V  TVAES         F+ L
Sbjct: 66  KSSLSSSLSHYLPLAGHIIW--EPHDPKPKIVY-TQNDAVSFTVAESNSD------FSLL 125

Query: 123 IADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGM 182
               P  +  L+P VP L      +D +  V  ++ Q+T+FPN+G C  V  HH  +DG 
Sbjct: 126 TGKEPFSSTELHPLVPELQN----SDDSAAV--VSFQVTLFPNQGFCIGVTTHHAVSDGK 185

Query: 183 AAHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESIFLQELSGWASSWN 242
               F+KSWA + +   +     L    P +DR  +K P ++D + + +     W S   
Sbjct: 186 TTTTFLKSWAHLCKHQDSSLPDDL---IPFYDRTVIKGPPEIDTKVLKI-----WHSIHK 245

Query: 243 PVPDKPI-KDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGPLRLSAFVVTCSLI 302
           P   K + +    +D  R TF LT+  IE+L+  +   +        +RLS FV+T S +
Sbjct: 246 PKSLKLLPRPEIESDVVRYTFELTRENIEKLRDKLKRES---SSFSSVRLSTFVITFSYV 305

Query: 303 WVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKD-PIPFTYFGNCIDICV 362
           + C+I       G+G        D  + P    FA DCR  + D PIP TYFGNC+   V
Sbjct: 306 FTCLI-------GSG-------GDDPNRPVGYRFAVDCRRLIDDPPIPLTYFGNCVYSAV 365

Query: 363 -VELKKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGRRLAITGSP 422
            + L     L     V  AR I + V+E +S       +    + K     + +++ GS 
Sbjct: 366 KIPLDAGMFLGEQGFVVAARLISDSVEELDSNVAWKIPELLETYEKAPVDSQFVSVAGST 425

Query: 423 RLRVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDRFVAMWDA 481
           R  +Y  DFGWGKP KS ++++D    + + ESRDG GG+E+G  L K  M+  + +   
Sbjct: 426 RFGIYGLDFGWGKPFKSLLVSIDQRGKISIAESRDGSGGVEIGFSLKKQEMNVLIDLLHK 448

BLAST of MC02g0509 vs. TAIR 10
Match: AT3G29670.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 223.4 bits (568), Expect = 3.9e-58
Identity = 159/489 (32.52%), Postives = 243/489 (49.69%), Query Frame = 0

Query: 3   NIVNECWVSPPAGSVATT----NLPLSFFDMLWIPNPPMRRFFFYDFPHSTH-HFMDAIF 62
           +++    V+P   SV  +     LPL+FFD+ W+   P++R FFY+   ST  HF   I 
Sbjct: 4   HVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSIIL 63

Query: 63  PTLTRSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVF 122
           P L  SLSL+L+++ PL G++ +  +P+  KP I+  + +  V +T+AES         F
Sbjct: 64  PKLKDSLSLILRNYLPLTGHITW--EPNEPKPSII-VSENGVVLVTIAESDAD------F 123

Query: 123 NRLIADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAA 182
           + L     R    L+  VP LP    ++D +      ++QIT+FPN+G    VA HH   
Sbjct: 124 SHLSGYGQRPLSELHALVPKLP----VSDDSATA--FSIQITLFPNQGFSIGVAAHHAVL 183

Query: 183 DGMAAHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESI----FLQELS 242
           DG  +  FIK+WA + +  +     +L    P++DR+ +K P  LD + I     L+E  
Sbjct: 184 DGKTSSTFIKAWAQICKQELQSMPENL---TPSYDRSLIKYPTYLDEKMIELVRSLKEDQ 243

Query: 243 GWASSWNPVPDKPIKDLAPTDKDRATFVLTKAQIERLKTWVSARAVNDEELGP-LRLSAF 302
               S   +P   + D    D   AT VL++A IERL+  V       + + P L LS F
Sbjct: 244 TNIRSLTSLPSSKLGD----DVVLATLVLSRADIERLREQV-------KNVSPSLHLSTF 303

Query: 303 VVTCSLIWVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYFGN 362
           V+  +  W C +++       GN          D    L+F  D R+RL   +P TYFGN
Sbjct: 304 VIAYAYAWTCFVKAR-----GGN---------KDRSVSLLFVGDFRDRLDPKLPGTYFGN 363

Query: 363 C-IDICVVELKKNEILKRHSIVGVARAIGERVKEFESEPLKT-ADKWFSAWRKCEESGRR 422
           C I +     K  E ++    V  A  I + VK   S  ++T AD +   +     S + 
Sbjct: 364 CMIPVGCYNRKAAEFMEEKGFVTAAEIISDLVKGLSSRKIETIADTFVEGFSFQSWSTQF 423

Query: 423 LAITGSPRLRVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMDR 480
             I GS RL VY+ DFGWG+P K +I+++D    + + E RD  GG+E+G+ L KT MD 
Sbjct: 424 GTIAGSTRLGVYEADFGWGRPVKVDIVSIDQGEAIAMAERRDESGGVEIGMCLKKTEMDS 449

BLAST of MC02g0509 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 222.6 bits (566), Expect = 6.7e-58
Identity = 155/491 (31.57%), Postives = 230/491 (46.84%), Query Frame = 0

Query: 4   IVNECWVSPP-AGSVATTNLPLSFFDMLWIPNPPMRRFFFYDFPHSTHHFMDA-IFPTLT 63
           +++   V+P  + S  +  LPL+FFD+LW     + R  FY    ++  F D+ I P L 
Sbjct: 12  VIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVPNLK 71

Query: 64  RSLSLVLQHFFPLAGNLIFPSQPHTHKPHILYTTAHDSVPLTVAESPQTQSGCDVFNRLI 123
            SLS  L H+ PLAG L++  +P   KP I+Y T +D+V  TVAES         F+RL 
Sbjct: 72  TSLSSSLSHYLPLAGKLVW--EPLDPKPKIVY-TPNDAVSFTVAESNAD------FSRLT 131

Query: 124 ADYPRHAKHLYPFVPGLPKPRVLADGTRVVPLLALQITVFPNRGLCFCVAFHHVAADGMA 183
              P     LYP VP L    V  D    V   + Q+T+FPN+G C  V  HH   DG  
Sbjct: 132 GKEPFPTTELYPLVPEL---HVSDDSASAV---SFQVTLFPNQGFCISVNAHHAVLDGKT 191

Query: 184 AHHFIKSWASMYRDLVAGGSGSLHGPPPNHDRAAVKDPNDLDLESI-----FLQELSGWA 243
             +F+KSWA   ++  +     L    P +DR  +KDP DLD + +       +  +G  
Sbjct: 192 TTNFLKSWARTCKNQDSFLPQDL---IPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGK 251

Query: 244 SSWNPVPDKPI--KDLAPTDKDRATFVLTKAQI----ERLKTWVSARAVNDEELGPLRLS 303
              NP   K +   ++ P D  R T  LT+  I    ERLK   S+ +V+      LRLS
Sbjct: 252 EPENPKSLKLLWSPEIGP-DVFRYTLNLTREDIQKLRERLKKESSSSSVSSSP-KELRLS 311

Query: 304 AFVVTCSLIWVCMIQSTESELGNGNAICNFEADKSDEPCYLVFAADCRNRLKDPIPFTYF 363
            FV+  S    C+I++   +                 P    FA DCR+ +  P+P +YF
Sbjct: 312 TFVIVYSYALTCLIKARGGD--------------PSRPVGYGFAVDCRSLMVPPVPSSYF 371

Query: 364 GNCIDICV-VELKKNEILKRHSIVGVARAIGERVKEFESEPLKTADKWFSAWRKCEESGR 423
           GNC+  C  + L     +     +  AR + + V+  +        +    +       +
Sbjct: 372 GNCVSACFKMSLTAETFMSEEGFLAAARMVSDSVEALDENVALKIPEILEGFTTLSPGTQ 431

Query: 424 RLAITGSPRLRVYDTDFGWGKPQKSEILNVDSSRLVCLQESRDGKGGIEVGLGLTKTRMD 481
            L++ GS R  VY  DFGWG+P+K  ++++D    +   ESRDG GG+E+G  L K  MD
Sbjct: 432 VLSVAGSTRFGVYGLDFGWGRPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMD 468

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LR731.7e-8536.42Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabid... [more]
Q9ZWB41.2e-8336.70Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabid... [more]
Q9MBC11.3e-6933.40Anthocyanidin 3-O-glucoside 6''-O-acyltransferase (Fragment) OS=Perilla frutesce... [more]
Q589Y01.8e-6833.68Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=... [more]
Q9ZWR82.2e-6632.93Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022140373.11.19e-23268.45LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltr... [more]
XP_022971001.19.23e-21962.86coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
XP_022971000.12.49e-21662.32coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
XP_023533558.16.85e-21662.32coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
KAG6604829.12.89e-21562.03Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1, partial ... [more]
Match NameE-valueIdentityDescription
A0A6J1CHV45.75e-23368.45LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltr... [more]
A0A6J1I2394.47e-21962.86coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
A0A6J1I4H81.21e-21662.32coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
A0A6J1G8652.09e-21361.65coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
A0A6J1G6S51.03e-21261.49coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
Match NameE-valueIdentityDescription
AT1G03495.11.2e-8636.42HXXXD-type acyl-transferase family protein [more]
AT1G03940.18.4e-8536.70HXXXD-type acyl-transferase family protein [more]
AT5G39090.12.0e-6232.51HXXXD-type acyl-transferase family protein [more]
AT3G29670.13.9e-5832.52HXXXD-type acyl-transferase family protein [more]
AT5G39050.16.7e-5831.57HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 2..239
e-value: 6.1E-59
score: 201.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 251..482
e-value: 1.8E-49
score: 169.8
IPR003480TransferasePFAMPF02458Transferasecoord: 13..472
e-value: 2.9E-37
score: 128.3
NoneNo IPR availablePANTHERPTHR31625FAMILY NOT NAMEDcoord: 3..483
NoneNo IPR availablePANTHERPTHR31625:SF52COUMAROYL-COA:ANTHOCYANIDIN 3-O-GLUCOSIDE-6''-O-COUMAROYLTRANSFERASE 1-RELATEDcoord: 3..483
NoneNo IPR availableSUPERFAMILY52777CoA-dependent acyltransferasescoord: 147..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0509.1MC02g0509.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups