MC02g0500 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0500
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptioncoumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like
LocationMC02: 4087214 .. 4088236 (-)
RNA-Seq ExpressionMC02g0500
SyntenyMC02g0500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTTCCACGCCCTTATAGCCGACCATGCAAAAGATGCCACAGACGTGTATCAATTATGTCAGGACTGGCCCAATTTCCGCGTCGCCTCGGATGGGACGACTCAGGCGATTCCTCTTCTCACACTGCGAGTCACCATCTTCCCCTACCAAGGCCTCTCCATTGGACTGTCGTTCCTCCACGTCGCCGTCGACGGACCCGCATTCCACCATTTCGTCAAATCATGGGCGTCCATCTGTAAAGGGCAAGACTTCGATGGGCCAACGCCGGTTCACGATCGGACTGTGGTTCGAGACCCGAATGGACTTGAATCGACCATTCTCCAGCTACAGTGGAGCATGGCTGGGCCCATCAATTTCGGGAGTGAGCCCATCAGTAAGCGTCTGGCCGACATGGTTCGGGCGACATTTGAGCTGTCGAAAGCCCAAATTGAGGGACTGAAGAGCTGGGCCAGGGCGGAATCTCCACATTTTTCGAGTTTCGTTGCAGTATGCTCTCTTGTTTGGGTTTGTTTCATCAAATCGCGTGAAGGCGAAGGAGGAAATGGAAAAGGAAGTGAAGTTTGTTGCTTCGCATTCGCAGCAGACTGCCGTAAGCGACTTGGATATCCGATGCCTGCGGGGTATTTCGGGAATTGCCTTGCGTTTTGCCATGCGGTAGTAGAGAAGGGGGAGCTTTTGGGTGGAGATGGTGTAATTGTGGGAATGAAGGCCACTGGGAAAAGAGTGGGAGAAGCGGAGGATGAGCCGTTGAAAGAGACGGCGGAGTGGGCGTCGGTTTGGAAGAAGTATAAAGAGCGTGGAGTTGTGTTCGCAGTGGCTGGGTCGCCGAGGTTGGGTGCGTACGATGTGGACTTTGGGTGGGGGAGGCCCATAAAAACTGATGTGATTCACATAGATTCAGGGAGCCTATTTTCTCTGGGAGAATCGAGAAATGAGAATGGTGGAATCGAGGTTGGGTTGGCTCTCAGTACCGCTCACATGGCTCGCTTTGTTGCCGTGTGGGAACAAACACTGAATCTTTTC

mRNA sequence

CACTTCCACGCCCTTATAGCCGACCATGCAAAAGATGCCACAGACGTGTATCAATTATGTCAGGACTGGCCCAATTTCCGCGTCGCCTCGGATGGGACGACTCAGGCGATTCCTCTTCTCACACTGCGAGTCACCATCTTCCCCTACCAAGGCCTCTCCATTGGACTGTCGTTCCTCCACGTCGCCGTCGACGGACCCGCATTCCACCATTTCGTCAAATCATGGGCGTCCATCTGTAAAGGGCAAGACTTCGATGGGCCAACGCCGGTTCACGATCGGACTGTGGTTCGAGACCCGAATGGACTTGAATCGACCATTCTCCAGCTACAGTGGAGCATGGCTGGGCCCATCAATTTCGGGAGTGAGCCCATCAGTAAGCGTCTGGCCGACATGGTTCGGGCGACATTTGAGCTGTCGAAAGCCCAAATTGAGGGACTGAAGAGCTGGGCCAGGGCGGAATCTCCACATTTTTCGAGTTTCGTTGCAGTATGCTCTCTTGTTTGGGTTTGTTTCATCAAATCGCGTGAAGGCGAAGGAGGAAATGGAAAAGGAAGTGAAGTTTGTTGCTTCGCATTCGCAGCAGACTGCCGTAAGCGACTTGGATATCCGATGCCTGCGGGGTATTTCGGGAATTGCCTTGCGTTTTGCCATGCGGTAGTAGAGAAGGGGGAGCTTTTGGGTGGAGATGGTGTAATTGTGGGAATGAAGGCCACTGGGAAAAGAGTGGGAGAAGCGGAGGATGAGCCGTTGAAAGAGACGGCGGAGTGGGCGTCGGTTTGGAAGAAGTATAAAGAGCGTGGAGTTGTGTTCGCAGTGGCTGGGTCGCCGAGGTTGGGTGCGTACGATGTGGACTTTGGGTGGGGGAGGCCCATAAAAACTGATGTGATTCACATAGATTCAGGGAGCCTATTTTCTCTGGGAGAATCGAGAAATGAGAATGGTGGAATCGAGGTTGGGTTGGCTCTCAGTACCGCTCACATGGCTCGCTTTGTTGCCGTGTGGGAACAAACACTGAATCTTTTC

Coding sequence (CDS)

CACTTCCACGCCCTTATAGCCGACCATGCAAAAGATGCCACAGACGTGTATCAATTATGTCAGGACTGGCCCAATTTCCGCGTCGCCTCGGATGGGACGACTCAGGCGATTCCTCTTCTCACACTGCGAGTCACCATCTTCCCCTACCAAGGCCTCTCCATTGGACTGTCGTTCCTCCACGTCGCCGTCGACGGACCCGCATTCCACCATTTCGTCAAATCATGGGCGTCCATCTGTAAAGGGCAAGACTTCGATGGGCCAACGCCGGTTCACGATCGGACTGTGGTTCGAGACCCGAATGGACTTGAATCGACCATTCTCCAGCTACAGTGGAGCATGGCTGGGCCCATCAATTTCGGGAGTGAGCCCATCAGTAAGCGTCTGGCCGACATGGTTCGGGCGACATTTGAGCTGTCGAAAGCCCAAATTGAGGGACTGAAGAGCTGGGCCAGGGCGGAATCTCCACATTTTTCGAGTTTCGTTGCAGTATGCTCTCTTGTTTGGGTTTGTTTCATCAAATCGCGTGAAGGCGAAGGAGGAAATGGAAAAGGAAGTGAAGTTTGTTGCTTCGCATTCGCAGCAGACTGCCGTAAGCGACTTGGATATCCGATGCCTGCGGGGTATTTCGGGAATTGCCTTGCGTTTTGCCATGCGGTAGTAGAGAAGGGGGAGCTTTTGGGTGGAGATGGTGTAATTGTGGGAATGAAGGCCACTGGGAAAAGAGTGGGAGAAGCGGAGGATGAGCCGTTGAAAGAGACGGCGGAGTGGGCGTCGGTTTGGAAGAAGTATAAAGAGCGTGGAGTTGTGTTCGCAGTGGCTGGGTCGCCGAGGTTGGGTGCGTACGATGTGGACTTTGGGTGGGGGAGGCCCATAAAAACTGATGTGATTCACATAGATTCAGGGAGCCTATTTTCTCTGGGAGAATCGAGAAATGAGAATGGTGGAATCGAGGTTGGGTTGGCTCTCAGTACCGCTCACATGGCTCGCTTTGTTGCCGTGTGGGAACAAACACTGAATCTTTTC

Protein sequence

HFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESPHFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF
Homology
BLAST of MC02g0500 vs. ExPASy Swiss-Prot
Match: Q9LR73 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=3AT2 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.0e-49
Identity = 119/353 (33.71%), Postives = 182/353 (51.56%), Query Frame = 0

Query: 2   FHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHV 61
           F  L +D  KD + ++ +    P   V+ +G  Q  P++ ++VTIFP  G+ IG S  HV
Sbjct: 116 FDQLKSDSPKDISVLHGVLPKLPPPHVSPEG-IQMRPIMAMQVTIFPGAGICIGNSATHV 175

Query: 62  AVDGPAFHHFVKSWASICKGQDFDGPT------PVHD-RTVVRDPNGLESTILQLQWSMA 121
             DG  F HF+K W S+ K    D  T      P+H  R +++DP  + +  L+  WS  
Sbjct: 176 VADGVTFSHFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHLERFWSQ- 235

Query: 122 GPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESPH---FSSFVAVCSLVWVCF 181
              N           +MVRATF LS+ QI+ LKSW   +S +    S+FV   + +WV  
Sbjct: 236 ---NSAKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSL 295

Query: 182 IKSREGEGGNGKGSEVCCFAFAADCRKRLGY--PMPAGYFGNCLAFCHAVVEKGELLGGD 241
           IK+   +       EV       DCR RL Y  P+P  YFGNC+A     V+K +LLG  
Sbjct: 296 IKTLVQDSETEAKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEK 355

Query: 242 GVIVGMKATGKRVGEAEDEPLKETA-EWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWG 301
            V+    A   R+ +     L +TA  W    +K+       ++AG+P+LG YD+DFG G
Sbjct: 356 CVMAASDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLG 415

Query: 302 RPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 342
           +P K +++HI++G   +  ESR+ + G+E+G+AL    M  F ++ ++ +  F
Sbjct: 416 KPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSLLQKGIKKF 463

BLAST of MC02g0500 vs. ExPASy Swiss-Prot
Match: Q9ZWB4 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=3AT1 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.8e-49
Identity = 121/357 (33.89%), Postives = 183/357 (51.26%), Query Frame = 0

Query: 2   FHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHV 61
           F  L +D  KD + ++ +    P   V+ +G  Q  P++ ++VTIFP  G+ IG S  HV
Sbjct: 116 FDQLKSDSPKDISVLHGVLPKLPPPHVSPEG-IQMRPIMAMQVTIFPGAGICIGNSATHV 175

Query: 62  AVDGPAFHHFVKSWASICKGQDFDGPT------PVHD-RTVVRDPNGLESTILQLQWSMA 121
             DG  F HF+K W S+ K    D  T      P+H  R +++DP  + +  L+  WS  
Sbjct: 176 VADGVTFSHFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHLERFWSQ- 235

Query: 122 GPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESPH---FSSFVAVCSLVWVCF 181
              N           +MVRATF LS+ QI+ LKSW   +S +    S+FV   + +WV  
Sbjct: 236 ---NSAKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSL 295

Query: 182 IKS----REGEGGNGKGSEVCCFAFAADCRKRLGY--PMPAGYFGNCLAFCHAVVEKGEL 241
           IK+     E +       EV       DCR RL Y  P+P  YFGNC+A     V+K +L
Sbjct: 296 IKTLVQDSETKANEEDKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDL 355

Query: 242 LGGDGVIVGMKATGKRVGEAEDEPLKETA-EWASVWKKYKERGVVFAVAGSPRLGAYDVD 301
           LG   V+    A   R+ +     L +TA  W    +K+       ++AG+P+LG YD+D
Sbjct: 356 LGEKCVLAASDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMD 415

Query: 302 FGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 342
           FG G+P K +++HI++G   +  ESR+ + G+E+G+AL    M  F ++ +Q +  F
Sbjct: 416 FGLGKPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGIKKF 467

BLAST of MC02g0500 vs. ExPASy Swiss-Prot
Match: Q9LRQ7 (BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.5e-45
Identity = 123/334 (36.83%), Postives = 176/334 (52.69%), Query Frame = 0

Query: 24  PNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICK--- 83
           P  +V+SD  +    LL+L++T+FP QG SIG +  HV +DG     F KSWA ICK   
Sbjct: 129 PELQVSSDSAS----LLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICKHGT 188

Query: 84  -GQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG---SEPISKRL-ADMVRAT 143
             QDFD PT V DRTV+  P GLE  I QL   ++   ++    + P +K +  D+VR T
Sbjct: 189 TPQDFDLPT-VLDRTVINVPAGLEQKIFQLSSYISEEKDYARTLTLPPAKEIDNDVVRVT 248

Query: 144 FELSKAQIEGLKSWARAESP----HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFA 203
            EL++  IE LK  A+ ES     H S+FV   + V  C +KS  G+      +    F 
Sbjct: 249 LELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGD-----ANRPVRFM 308

Query: 204 FAADCRKRLGYPMPAGYFGNC-LAFCHAVVEKGELLGGDGVIVGMKATGKRV---GEAED 263
           +AAD R RL  P+P  YFGNC L       +    LG DG + G++     V   G    
Sbjct: 309 YAADFRNRLDPPVPLTYFGNCVLPIDFNGYKATTFLGKDGYVNGVEILSDSVRGLGSRNI 368

Query: 264 EPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLG 323
           E + E  E  +   K   + V   V GS + G Y  DFGWGRP+KTDV+ +   + FS+ 
Sbjct: 369 ESIWEVYEDGTKNMKLDTQNV--TVTGSNQFGIYGSDFGWGRPVKTDVMSLYKNNEFSMS 428

Query: 324 ESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 342
             R+E GG+E+G++L    M  F++++    +++
Sbjct: 429 ARRDEIGGLEIGISLKKCEMNVFLSLFTSDFDIY 450

BLAST of MC02g0500 vs. ExPASy Swiss-Prot
Match: Q9LRQ8 (Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 7.1e-43
Identity = 107/327 (32.72%), Postives = 163/327 (49.85%), Query Frame = 0

Query: 24  PNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQD 83
           P   V+ D  T      ++++T+FP QG SIG++  H  +DG     F+K+WA ICK + 
Sbjct: 133 PKLPVSDDSAT----AFSIQITLFPNQGFSIGVAAHHAVLDGKTSSTFIKAWAQICKQEL 192

Query: 84  FDGP---TPVHDRTVVRDPNGLESTILQLQWSM----AGPINFGSEPISKRLADMVRATF 143
              P   TP +DR++++ P  L+  +++L  S+        +  S P SK   D+V AT 
Sbjct: 193 QSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTSLPSSKLGDDVVLATL 252

Query: 144 ELSKAQIEGLKSWARAESP--HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAA 203
            LS+A IE L+   +  SP  H S+FV   +  W CF+K+R   GGN   S      F  
Sbjct: 253 VLSRADIERLREQVKNVSPSLHLSTFVIAYAYAWTCFVKAR---GGNKDRS--VSLLFVG 312

Query: 204 DCRKRLGYPMPAGYFGNCLAFCHAVVEK-GELLGGDGVIVGMKATGKRVGEAEDEPLKET 263
           D R RL   +P  YFGNC+        K  E +   G +   +     V       ++  
Sbjct: 313 DFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEIISDLVKGLSSRKIETI 372

Query: 264 AEWASVWKKYKERGVVF-AVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNE 323
           A+       ++     F  +AGS RLG Y+ DFGWGRP+K D++ ID G   ++ E R+E
Sbjct: 373 ADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVSIDQGEAIAMAERRDE 432

Query: 324 NGGIEVGLALSTAHMARFVAVWEQTLN 340
           +GG+E+G+ L    M   V+ +   L+
Sbjct: 433 SGGVEIGMCLKKTEMDSVVSFFNNGLH 450

BLAST of MC02g0500 vs. ExPASy Swiss-Prot
Match: Q589Y0 (Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.3e-43
Identity = 117/352 (33.24%), Postives = 174/352 (49.43%), Query Frame = 0

Query: 2   FHALIADHAKDATDVYQLCQDWPNFRVASDGT-TQAIPLLTLRVTIFPYQGLSIGLSFLH 61
           F+ LI  H ++  D Y      P      D    Q  P+L ++VT+FP  G+SIG +  H
Sbjct: 109 FNYLIGYHPRNTKDFYHFV---PQLAEPKDAPGVQLAPVLAIQVTLFPNHGISIGFTNHH 168

Query: 62  VAVDGPAFHHFVKSWASICKGQDFDGP--------TPVHDRTVVRDPNGLESTILQLQWS 121
           VA DG     FV++WA + K   F G          P +DR+V++DPNG+  +I      
Sbjct: 169 VAGDGATIVKFVRAWALLNK---FGGDEQFLANEFIPFYDRSVIKDPNGVGMSIWNEMKK 228

Query: 122 MAGPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESP---HFSSFVAVCSLVWV 181
               +   S+ ++    D VR TF +++  I  LK+      P   H +SF   C+ VW 
Sbjct: 229 YKHMMKM-SDVVTP--PDKVRGTFIITRHDIGKLKNLVLTRRPKLTHVTSFTVTCAYVWT 288

Query: 182 CFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGD 241
           C IKS    G     + +  F  AADCR +   P+P  YFGN L    A   + +L G +
Sbjct: 289 CIIKSEAATGEEIDENGMEFFGCAADCRAQFNPPLPPSYFGNALVGYVARTRQVDLAGKE 348

Query: 242 GVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGVV-----FAVAGSPRLGAYDVD 301
           G  + ++     +GEA  + +K+  EW      +KE   V      +VAGSP+L  Y  D
Sbjct: 349 GFTIAVEL----IGEAIRKRMKD-EEWILSGSWFKEYDKVDAKRSLSVAGSPKLDLYAAD 408

Query: 302 FGWGRPIKTDVIHIDS--GSLFSLGESRNENGGIEVGLALSTAHMARFVAVW 335
           FGWGRP K + + ID+  G   SL +S++ +G +E+GL+LS   M  F A++
Sbjct: 409 FGWGRPEKLEFVSIDNDDGISMSLSKSKDSDGDLEIGLSLSKTRMNAFAAMF 446

BLAST of MC02g0500 vs. NCBI nr
Match: XP_022140618.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Momordica charantia])

HSP 1 Score: 712 bits (1837), Expect = 1.46e-258
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   HFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLH 60
           HFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLH
Sbjct: 39  HFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLH 98

Query: 61  VAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG 120
           VAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG
Sbjct: 99  VAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG 158

Query: 121 SEPISKRLADMVRATFELSKAQIEGLKSWARAESPHFSSFVAVCSLVWVCFIKSREGEGG 180
           SEPISKRLADMVRATFELSKAQIEGLKSWARAESPHFSSFVAVCSLVWVCFIKSREGEGG
Sbjct: 159 SEPISKRLADMVRATFELSKAQIEGLKSWARAESPHFSSFVAVCSLVWVCFIKSREGEGG 218

Query: 181 NGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGK 240
           NGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGK
Sbjct: 219 NGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGK 278

Query: 241 RVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDS 300
           RVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDS
Sbjct: 279 RVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDS 338

Query: 301 GSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 341
           GSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF
Sbjct: 339 GSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 379

BLAST of MC02g0500 vs. NCBI nr
Match: XP_023512859.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432 bits (1110), Expect = 9.64e-147
Identity = 217/345 (62.90%), Postives = 251/345 (72.75%), Query Frame = 0

Query: 1   HFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLH 60
           HFHALI DH KDA DVY L QDWP   + SDGT Q IPL+ L++TIFPY  LS+ +SFLH
Sbjct: 115 HFHALIPDHPKDARDVYPLFQDWPMSCLLSDGT-QVIPLMALQITIFPYHALSLCVSFLH 174

Query: 61  VAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG 120
           VA DGPA HHF++SWASI  G   +GP P HDRTVV+DP GLES  L+     + PINF 
Sbjct: 175 VAADGPALHHFIRSWASISAGNGLNGPLPSHDRTVVQDPEGLESAYLRHH---SEPINFD 234

Query: 121 SEPISKRLADMVRATFELSKAQIEGLKSWARAE---SPHFSSFVAVCSLVWVCFIKSREG 180
           SEPIS+RL+D VRATF LSK QIE LK+WA ++   S  FSSFV   S+VWV FI SRE 
Sbjct: 235 SEPISQRLSDKVRATFSLSKPQIERLKNWANSQITKSTQFSSFVVTSSVVWVSFIMSREL 294

Query: 181 -EGGNGKGSEVCCFAFAADCR-KRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGM 240
             GG+G   E CCFAFAA+CR +RLGY +P  YFGNC AFC   VEK ELLG +G++V M
Sbjct: 295 ISGGSGAEDEACCFAFAANCRNERLGYRIPVEYFGNCFAFCLTAVEKSELLGENGLVVAM 354

Query: 241 KATGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDV 300
           KA  KRVGE   E LK+     S WK++ ERG     AGSPRLGAYD +FGWGRPIKT+V
Sbjct: 355 KAIEKRVGEVNKETLKDVMAEMSAWKEFVERGAALCAAGSPRLGAYDANFGWGRPIKTEV 414

Query: 301 IHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNL 340
           + +D G LFS+GESRNENGGIEVGL L +AHMA FVAVWEQ L L
Sbjct: 415 VQVDRGRLFSIGESRNENGGIEVGLGLGSAHMASFVAVWEQNLKL 455

BLAST of MC02g0500 vs. NCBI nr
Match: XP_022986200.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Cucurbita maxima])

HSP 1 Score: 348 bits (892), Expect = 4.62e-116
Identity = 171/277 (61.73%), Postives = 200/277 (72.20%), Query Frame = 0

Query: 69  HHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFGSEPISKRL 128
           HHF++SWASI  G   +GP P HDRTVV+DP GLES  L+  WS+A PI   SEPIS+RL
Sbjct: 17  HHFIRSWASISAGNGLNGPLPSHDRTVVQDPEGLESAFLRHHWSVAEPIFLDSEPISQRL 76

Query: 129 ADMVRATFELSKAQIEGLKSWARAE---SPHFSSFVAVCSLVWVCFIKSREG-EGGNGKG 188
           +D VRATF LSK QI  LK+WA ++   S  FSSFV  CS+VWV FI SRE   GG G  
Sbjct: 77  SDKVRATFSLSKPQISRLKNWANSQITKSTQFSSFVVTCSVVWVSFIMSRELISGGCGAE 136

Query: 189 SEVCCFAFAADCR-KRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGKRVG 248
            E CCFAF A+CR +RLGY +PA YFGNC AFC   VEK ELLG +G++V MKA  KRVG
Sbjct: 137 DEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIEKRVG 196

Query: 249 EAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSL 308
           E + E LK+     +VWK++ ERG +    GSPRLGAYD  FGWGRPIKT+V+ +D G L
Sbjct: 197 EVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRPIKTEVVQVDRGRL 256

Query: 309 FSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNL 340
           FS+GESRNENGGIEVGL L + HMA FVAVWEQ L L
Sbjct: 257 FSIGESRNENGGIEVGLGLGSTHMASFVAVWEQNLKL 293

BLAST of MC02g0500 vs. NCBI nr
Match: KAG7010704.1 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 323 bits (827), Expect = 4.06e-106
Identity = 165/273 (60.44%), Postives = 196/273 (71.79%), Query Frame = 0

Query: 27  RVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQDFDG 86
           R+ SDGT Q IPL+ L++TIFPY GLS+ +SFLHVA DGPA HHF++SWASI  G   +G
Sbjct: 4   RLLSDGT-QVIPLMALQITIFPYHGLSLCVSFLHVAADGPALHHFIRSWASISAGNGLNG 63

Query: 87  PTPVHDRTVVRDPNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFELSKAQIEGL 146
           P P HDRTVV+DP GLES  L+     + PINF SE +S+RL+D VRATF LSK QIE L
Sbjct: 64  PLPSHDRTVVQDPEGLESAYLRHH---SEPINFDSETMSQRLSDKVRATFSLSKPQIERL 123

Query: 147 KSWARAE---SPHFSSFVAVCSLVWVCFIKSREG-EGGNGKGSEVCCFAFAADCR-KRLG 206
           K+WA ++   S  FSSFV   S+VWV FI SRE   GG+G   E CCFAFAA+CR +RLG
Sbjct: 124 KNWANSQITKSTQFSSFVVTSSVVWVSFIMSRELISGGSGAEDEACCFAFAANCRNERLG 183

Query: 207 YPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGKRVGEAEDEPLKETAEWASVWK 266
           Y +PA YFGNC AFC   VEK ELLG +G++V MKA  KRVGE +   LK+     SVWK
Sbjct: 184 YRIPAEYFGNCFAFCLTAVEKSELLGENGLVVAMKAIEKRVGEVKKVTLKDVMAEMSVWK 243

Query: 267 KYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTD 294
           +  ERG     AGSPRLGAYD +FGWGRPIKT+
Sbjct: 244 ELVERGAALCAAGSPRLGAYDTNFGWGRPIKTE 272

BLAST of MC02g0500 vs. NCBI nr
Match: KAE8075836.1 (hypothetical protein FH972_014523 [Carpinus fangiana])

HSP 1 Score: 325 bits (834), Expect = 6.20e-105
Identity = 166/353 (47.03%), Postives = 221/353 (62.61%), Query Frame = 0

Query: 2   FHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHV 61
           F+ L+A++ +D  +++      P   V+SD T + IPL+ ++VT+FP +G+ IG+ F HV
Sbjct: 114 FNRLVANYPRDVRELHHFVPQLPPAHVSSDKT-RVIPLMAMQVTVFPNEGICIGIIFCHV 173

Query: 62  AVDGPAFHHFVKSWASICK-GQDF---DGPTPVHDRTVVRDPNGLESTILQLQWSMAGPI 121
           A DG AFHHF+KSWAS+C+ G D    D   P +DR V++DPNGLE  +L+  WS     
Sbjct: 174 AADGRAFHHFMKSWASVCRTGGDLTCLDRSPPFNDRAVMKDPNGLEQNLLEDWWSWGSAW 233

Query: 122 NFGSEPISKRLADMVRATFELSKAQIEGLKSWAR--------AESPHFSSFVAVCSLVWV 181
              +  I+   A+ VRATF LS+A IEGLK W +        +E  H S+FVA C+ +WV
Sbjct: 234 TDDTGLIND-FAEKVRATFVLSRAHIEGLKQWVKGQCMNKEDSEPLHVSTFVATCAFIWV 293

Query: 182 CFIKSR-EGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGG 241
           C IKS+  G       SE+  FAF ADCR RL +P+P  YFGNCLA C   +EKGELLG 
Sbjct: 294 CLIKSQYNGVSDFSDNSELFYFAFVADCRNRLKFPVPCTYFGNCLAICIVALEKGELLGE 353

Query: 242 DGVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWG 301
            G+   +K  G +V ++E E L+    W   WK+  E G +  VAGSPRLG Y+ DFGWG
Sbjct: 354 TGIFEAVKVIGNKVRQSETEALRGAETWLLDWKEKSETGTLVTVAGSPRLGVYETDFGWG 413

Query: 302 RPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 341
           RP K++V+HID     SL E R+E GGIEVGLAL   ++A F A WEQ+L LF
Sbjct: 414 RPKKSEVVHIDVTGAISLAECRDEEGGIEVGLALRRKNIADFTAAWEQSLKLF 464

BLAST of MC02g0500 vs. ExPASy TrEMBL
Match: A0A6J1CIE1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111011225 PE=3 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 7.09e-259
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   HFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLH 60
           HFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLH
Sbjct: 39  HFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLH 98

Query: 61  VAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG 120
           VAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG
Sbjct: 99  VAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG 158

Query: 121 SEPISKRLADMVRATFELSKAQIEGLKSWARAESPHFSSFVAVCSLVWVCFIKSREGEGG 180
           SEPISKRLADMVRATFELSKAQIEGLKSWARAESPHFSSFVAVCSLVWVCFIKSREGEGG
Sbjct: 159 SEPISKRLADMVRATFELSKAQIEGLKSWARAESPHFSSFVAVCSLVWVCFIKSREGEGG 218

Query: 181 NGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGK 240
           NGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGK
Sbjct: 219 NGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGK 278

Query: 241 RVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDS 300
           RVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDS
Sbjct: 279 RVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDS 338

Query: 301 GSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 341
           GSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF
Sbjct: 339 GSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 379

BLAST of MC02g0500 vs. ExPASy TrEMBL
Match: A0A6J1JDF0 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484014 PE=3 SV=1)

HSP 1 Score: 348 bits (892), Expect = 2.24e-116
Identity = 171/277 (61.73%), Postives = 200/277 (72.20%), Query Frame = 0

Query: 69  HHFVKSWASICKGQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFGSEPISKRL 128
           HHF++SWASI  G   +GP P HDRTVV+DP GLES  L+  WS+A PI   SEPIS+RL
Sbjct: 17  HHFIRSWASISAGNGLNGPLPSHDRTVVQDPEGLESAFLRHHWSVAEPIFLDSEPISQRL 76

Query: 129 ADMVRATFELSKAQIEGLKSWARAE---SPHFSSFVAVCSLVWVCFIKSREG-EGGNGKG 188
           +D VRATF LSK QI  LK+WA ++   S  FSSFV  CS+VWV FI SRE   GG G  
Sbjct: 77  SDKVRATFSLSKPQISRLKNWANSQITKSTQFSSFVVTCSVVWVSFIMSRELISGGCGAE 136

Query: 189 SEVCCFAFAADCR-KRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGKRVG 248
            E CCFAF A+CR +RLGY +PA YFGNC AFC   VEK ELLG +G++V MKA  KRVG
Sbjct: 137 DEACCFAFTANCRNERLGYRIPAEYFGNCFAFCLTTVEKSELLGENGLVVAMKAIEKRVG 196

Query: 249 EAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSL 308
           E + E LK+     +VWK++ ERG +    GSPRLGAYD  FGWGRPIKT+V+ +D G L
Sbjct: 197 EVKKETLKDAMAETTVWKEFVERGTMLCAVGSPRLGAYDTSFGWGRPIKTEVVQVDRGRL 256

Query: 309 FSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNL 340
           FS+GESRNENGGIEVGL L + HMA FVAVWEQ L L
Sbjct: 257 FSIGESRNENGGIEVGLGLGSTHMASFVAVWEQNLKL 293

BLAST of MC02g0500 vs. ExPASy TrEMBL
Match: A0A5N6RBV5 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_014523 PE=3 SV=1)

HSP 1 Score: 325 bits (834), Expect = 3.00e-105
Identity = 166/353 (47.03%), Postives = 221/353 (62.61%), Query Frame = 0

Query: 2   FHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHV 61
           F+ L+A++ +D  +++      P   V+SD T + IPL+ ++VT+FP +G+ IG+ F HV
Sbjct: 114 FNRLVANYPRDVRELHHFVPQLPPAHVSSDKT-RVIPLMAMQVTVFPNEGICIGIIFCHV 173

Query: 62  AVDGPAFHHFVKSWASICK-GQDF---DGPTPVHDRTVVRDPNGLESTILQLQWSMAGPI 121
           A DG AFHHF+KSWAS+C+ G D    D   P +DR V++DPNGLE  +L+  WS     
Sbjct: 174 AADGRAFHHFMKSWASVCRTGGDLTCLDRSPPFNDRAVMKDPNGLEQNLLEDWWSWGSAW 233

Query: 122 NFGSEPISKRLADMVRATFELSKAQIEGLKSWAR--------AESPHFSSFVAVCSLVWV 181
              +  I+   A+ VRATF LS+A IEGLK W +        +E  H S+FVA C+ +WV
Sbjct: 234 TDDTGLIND-FAEKVRATFVLSRAHIEGLKQWVKGQCMNKEDSEPLHVSTFVATCAFIWV 293

Query: 182 CFIKSR-EGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGG 241
           C IKS+  G       SE+  FAF ADCR RL +P+P  YFGNCLA C   +EKGELLG 
Sbjct: 294 CLIKSQYNGVSDFSDNSELFYFAFVADCRNRLKFPVPCTYFGNCLAICIVALEKGELLGE 353

Query: 242 DGVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWG 301
            G+   +K  G +V ++E E L+    W   WK+  E G +  VAGSPRLG Y+ DFGWG
Sbjct: 354 TGIFEAVKVIGNKVRQSETEALRGAETWLLDWKEKSETGTLVTVAGSPRLGVYETDFGWG 413

Query: 302 RPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 341
           RP K++V+HID     SL E R+E GGIEVGLAL   ++A F A WEQ+L LF
Sbjct: 414 RPKKSEVVHIDVTGAISLAECRDEEGGIEVGLALRRKNIADFTAAWEQSLKLF 464

BLAST of MC02g0500 vs. ExPASy TrEMBL
Match: A0A1Q3B9H9 (Transferase domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_08224 PE=3 SV=1)

HSP 1 Score: 308 bits (788), Expect = 4.30e-98
Identity = 156/352 (44.32%), Postives = 209/352 (59.38%), Query Frame = 0

Query: 3   HALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVA 62
           H +I +HA+D  +++      P   V+SDGT + +PLL ++VT+FP+ G+ IG+ F HVA
Sbjct: 131 HQVIGNHARDVRELHPFVPRLPPSCVSSDGT-RVLPLLAVQVTVFPFSGVCIGVEFSHVA 190

Query: 63  VDGPAFHHFVKSWASICKGQD-----FDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPI 122
            DG AF+HF+KSWASIC+         D   P H R  + DP G+ES +L+  W  A   
Sbjct: 191 ADGRAFNHFMKSWASICRSNGNSLTYVDNWLPAHGRDQIEDPQGIESILLKDWWDWASNW 250

Query: 123 NFGSEPISKRLADMVRATFELSKAQIEGLKSWARAE--------SPHFSSFVAVCSLVWV 182
                PI+  LAD VRAT  L + QIE LK W              H S+FV  CSL+WV
Sbjct: 251 EEDKSPINDVLADKVRATLVLGRGQIERLKLWVSLNCMNHNEQGQAHLSTFVVTCSLIWV 310

Query: 183 CFIKSREGEGGN-GKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGG 242
           C +KS+E E  N     E+C F F ADC  RL + +PA YFGNCLA C   ++  +++G 
Sbjct: 311 CSVKSQESEVSNLHNNDELCYFCFVADCCNRLDFSIPATYFGNCLAICVVTLKMSDIMGE 370

Query: 243 DGVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWG 302
           DG++   KA G +V E E   L+   +W S WK+  E G V  VAGSPRL  Y+ DFGWG
Sbjct: 371 DGIVAATKAIGNKVRELESGALRGADKWLSCWKEISEAGHVVTVAGSPRLRVYETDFGWG 430

Query: 303 RPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNL 340
           +PIK++V+HID      + ESR+E GGIE+GLALS A +  F A +E+ L L
Sbjct: 431 KPIKSEVVHIDMSRGIYICESRDEEGGIEIGLALSKAQINVFNAFFEEALML 481

BLAST of MC02g0500 vs. ExPASy TrEMBL
Match: A0A6J1CHV4 (LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111011063 PE=3 SV=1)

HSP 1 Score: 301 bits (771), Expect = 1.30e-95
Identity = 164/360 (45.56%), Postives = 213/360 (59.17%), Query Frame = 0

Query: 2   FHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHV 61
           F  LIADH + A   Y L  D PN R + DG  + +P+  L++T+FP QGL I ++F HV
Sbjct: 117 FDRLIADHPRPAKHFYPLVPDLPNSRASPDGA-RVVPVFALQITVFPNQGLCISIAFHHV 176

Query: 62  AVDGPAFHHFVKSWASICKGQ----DFDGPTPVHDRTVVRDPNGLESTILQ--LQW-SMA 121
             DG A H F+KS ASI +        DGP P HDR  V+DPNGLES  LQ    W S+ 
Sbjct: 177 VADGMAAHDFLKSXASIHRNGGGSGSLDGPLPYHDRAAVKDPNGLESIFLQELSYWDSLW 236

Query: 122 GPINFGSEPISKRL-ADMVRATFELSKAQIEGLKSWARAESPH----------FSSFVAV 181
            P+    +PI     AD VRATF ++K QIE LK+W   ++ H           S+ +  
Sbjct: 237 NPVP--DKPIIDLAPADKVRATFVINKTQIERLKTWISNQAAHDDNEELKPLRLSALIVT 296

Query: 182 CSLVWVCFIKSREGEGGNG----KGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAV 241
           CSL+W C IKS EGE        +  E+C F   ADCR RL  P+P  YFGNCL     V
Sbjct: 297 CSLIWGCLIKSTEGEFAQNDLPDELDELCFFVLLADCRNRLKDPLPPTYFGNCLDMGAVV 356

Query: 242 VEKGELLGGDGVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGSPRLG 301
           ++K ELL  +  +V  KA GKRV E E EPLK   +W S W+  + + ++  +AGSP+L 
Sbjct: 357 LKKSELLKSNSSVVAAKAIGKRVEEFESEPLKTADKWFSEWRCLESKHLL-TIAGSPKLR 416

Query: 302 AYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLN 339
            YD DFGWG+P K++V+H+DS    SL E R+E GGIEVGL L+  HM RF+A+WE+ LN
Sbjct: 417 VYDTDFGWGKPKKSEVLHVDSSLAISLQECRDEKGGIEVGLGLTKTHMDRFIAIWEENLN 472

BLAST of MC02g0500 vs. TAIR 10
Match: AT1G03495.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 198.7 bits (504), Expect = 7.3e-51
Identity = 119/353 (33.71%), Postives = 182/353 (51.56%), Query Frame = 0

Query: 2   FHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHV 61
           F  L +D  KD + ++ +    P   V+ +G  Q  P++ ++VTIFP  G+ IG S  HV
Sbjct: 116 FDQLKSDSPKDISVLHGVLPKLPPPHVSPEG-IQMRPIMAMQVTIFPGAGICIGNSATHV 175

Query: 62  AVDGPAFHHFVKSWASICKGQDFDGPT------PVHD-RTVVRDPNGLESTILQLQWSMA 121
             DG  F HF+K W S+ K    D  T      P+H  R +++DP  + +  L+  WS  
Sbjct: 176 VADGVTFSHFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHLERFWSQ- 235

Query: 122 GPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESPH---FSSFVAVCSLVWVCF 181
              N           +MVRATF LS+ QI+ LKSW   +S +    S+FV   + +WV  
Sbjct: 236 ---NSAKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSL 295

Query: 182 IKSREGEGGNGKGSEVCCFAFAADCRKRLGY--PMPAGYFGNCLAFCHAVVEKGELLGGD 241
           IK+   +       EV       DCR RL Y  P+P  YFGNC+A     V+K +LLG  
Sbjct: 296 IKTLVQDSETEAKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEK 355

Query: 242 GVIVGMKATGKRVGEAEDEPLKETA-EWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWG 301
            V+    A   R+ +     L +TA  W    +K+       ++AG+P+LG YD+DFG G
Sbjct: 356 CVMAASDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLG 415

Query: 302 RPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 342
           +P K +++HI++G   +  ESR+ + G+E+G+AL    M  F ++ ++ +  F
Sbjct: 416 KPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSLLQKGIKKF 463

BLAST of MC02g0500 vs. TAIR 10
Match: AT1G03940.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.2e-50
Identity = 121/357 (33.89%), Postives = 183/357 (51.26%), Query Frame = 0

Query: 2   FHALIADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHV 61
           F  L +D  KD + ++ +    P   V+ +G  Q  P++ ++VTIFP  G+ IG S  HV
Sbjct: 116 FDQLKSDSPKDISVLHGVLPKLPPPHVSPEG-IQMRPIMAMQVTIFPGAGICIGNSATHV 175

Query: 62  AVDGPAFHHFVKSWASICKGQDFDGPT------PVHD-RTVVRDPNGLESTILQLQWSMA 121
             DG  F HF+K W S+ K    D  T      P+H  R +++DP  + +  L+  WS  
Sbjct: 176 VADGVTFSHFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHLERFWSQ- 235

Query: 122 GPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESPH---FSSFVAVCSLVWVCF 181
              N           +MVRATF LS+ QI+ LKSW   +S +    S+FV   + +WV  
Sbjct: 236 ---NSAKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSL 295

Query: 182 IKS----REGEGGNGKGSEVCCFAFAADCRKRLGY--PMPAGYFGNCLAFCHAVVEKGEL 241
           IK+     E +       EV       DCR RL Y  P+P  YFGNC+A     V+K +L
Sbjct: 296 IKTLVQDSETKANEEDKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDL 355

Query: 242 LGGDGVIVGMKATGKRVGEAEDEPLKETA-EWASVWKKYKERGVVFAVAGSPRLGAYDVD 301
           LG   V+    A   R+ +     L +TA  W    +K+       ++AG+P+LG YD+D
Sbjct: 356 LGEKCVLAASDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMD 415

Query: 302 FGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 342
           FG G+P K +++HI++G   +  ESR+ + G+E+G+AL    M  F ++ +Q +  F
Sbjct: 416 FGLGKPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGIKKF 467

BLAST of MC02g0500 vs. TAIR 10
Match: AT3G29680.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.2e-46
Identity = 123/334 (36.83%), Postives = 176/334 (52.69%), Query Frame = 0

Query: 24  PNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICK--- 83
           P  +V+SD  +    LL+L++T+FP QG SIG +  HV +DG     F KSWA ICK   
Sbjct: 129 PELQVSSDSAS----LLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICKHGT 188

Query: 84  -GQDFDGPTPVHDRTVVRDPNGLESTILQLQWSMAGPINFG---SEPISKRL-ADMVRAT 143
             QDFD PT V DRTV+  P GLE  I QL   ++   ++    + P +K +  D+VR T
Sbjct: 189 TPQDFDLPT-VLDRTVINVPAGLEQKIFQLSSYISEEKDYARTLTLPPAKEIDNDVVRVT 248

Query: 144 FELSKAQIEGLKSWARAESP----HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFA 203
            EL++  IE LK  A+ ES     H S+FV   + V  C +KS  G+      +    F 
Sbjct: 249 LELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGD-----ANRPVRFM 308

Query: 204 FAADCRKRLGYPMPAGYFGNC-LAFCHAVVEKGELLGGDGVIVGMKATGKRV---GEAED 263
           +AAD R RL  P+P  YFGNC L       +    LG DG + G++     V   G    
Sbjct: 309 YAADFRNRLDPPVPLTYFGNCVLPIDFNGYKATTFLGKDGYVNGVEILSDSVRGLGSRNI 368

Query: 264 EPLKETAEWASVWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLG 323
           E + E  E  +   K   + V   V GS + G Y  DFGWGRP+KTDV+ +   + FS+ 
Sbjct: 369 ESIWEVYEDGTKNMKLDTQNV--TVTGSNQFGIYGSDFGWGRPVKTDVMSLYKNNEFSMS 428

Query: 324 ESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 342
             R+E GG+E+G++L    M  F++++    +++
Sbjct: 429 ARRDEIGGLEIGISLKKCEMNVFLSLFTSDFDIY 450

BLAST of MC02g0500 vs. TAIR 10
Match: AT3G29670.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 176.0 bits (445), Expect = 5.1e-44
Identity = 107/327 (32.72%), Postives = 163/327 (49.85%), Query Frame = 0

Query: 24  PNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQD 83
           P   V+ D  T      ++++T+FP QG SIG++  H  +DG     F+K+WA ICK + 
Sbjct: 133 PKLPVSDDSAT----AFSIQITLFPNQGFSIGVAAHHAVLDGKTSSTFIKAWAQICKQEL 192

Query: 84  FDGP---TPVHDRTVVRDPNGLESTILQLQWSM----AGPINFGSEPISKRLADMVRATF 143
              P   TP +DR++++ P  L+  +++L  S+        +  S P SK   D+V AT 
Sbjct: 193 QSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTSLPSSKLGDDVVLATL 252

Query: 144 ELSKAQIEGLKSWARAESP--HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAA 203
            LS+A IE L+   +  SP  H S+FV   +  W CF+K+R   GGN   S      F  
Sbjct: 253 VLSRADIERLREQVKNVSPSLHLSTFVIAYAYAWTCFVKAR---GGNKDRS--VSLLFVG 312

Query: 204 DCRKRLGYPMPAGYFGNCLAFCHAVVEK-GELLGGDGVIVGMKATGKRVGEAEDEPLKET 263
           D R RL   +P  YFGNC+        K  E +   G +   +     V       ++  
Sbjct: 313 DFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEIISDLVKGLSSRKIETI 372

Query: 264 AEWASVWKKYKERGVVF-AVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNE 323
           A+       ++     F  +AGS RLG Y+ DFGWGRP+K D++ ID G   ++ E R+E
Sbjct: 373 ADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVSIDQGEAIAMAERRDE 432

Query: 324 NGGIEVGLALSTAHMARFVAVWEQTLN 340
           +GG+E+G+ L    M   V+ +   L+
Sbjct: 433 SGGVEIGMCLKKTEMDSVVSFFNNGLH 450

BLAST of MC02g0500 vs. TAIR 10
Match: AT5G39090.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 173.7 bits (439), Expect = 2.5e-43
Identity = 103/299 (34.45%), Postives = 158/299 (52.84%), Query Frame = 0

Query: 39  LLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQDFDGP---TPVHDRTV 98
           +++ +VT+FP QG  IG++  H   DG     F+KSWA +CK QD   P    P +DRTV
Sbjct: 143 VVSFQVTLFPNQGFCIGVTTHHAVSDGKTTTTFLKSWAHLCKHQDSSLPDDLIPFYDRTV 202

Query: 99  VRDPNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESP 158
           ++ P  +++ +L++  S+  P +    P  +  +D+VR TFEL++  IE L+   + ES 
Sbjct: 203 IKGPPEIDTKVLKIWHSIHKPKSLKLLPRPEIESDVVRYTFELTRENIEKLRDKLKRESS 262

Query: 159 HFSS-----FVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAADCRKRL-GYPMPAGYF 218
            FSS     FV   S V+ C I    G GG+     V  + FA DCR+ +   P+P  YF
Sbjct: 263 SFSSVRLSTFVITFSYVFTCLI----GSGGDDPNRPV-GYRFAVDCRRLIDDPPIPLTYF 322

Query: 219 GNCLAFCHAV-VEKGELLGGDGVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGV 278
           GNC+     + ++ G  LG  G +V  +     V E +     +  E    ++K      
Sbjct: 323 GNCVYSAVKIPLDAGMFLGEQGFVVAARLISDSVEELDSNVAWKIPELLETYEKAPVDSQ 382

Query: 279 VFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHM 328
             +VAGS R G Y +DFGWG+P K+ ++ ID     S+ ESR+ +GG+E+G +L    M
Sbjct: 383 FVSVAGSTRFGIYGLDFGWGKPFKSLLVSIDQRGKISIAESRDGSGGVEIGFSLKKQEM 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LR731.0e-4933.71Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabid... [more]
Q9ZWB41.8e-4933.89Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabid... [more]
Q9LRQ74.5e-4536.83BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2... [more]
Q9LRQ87.1e-4332.72Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2... [more]
Q589Y09.3e-4333.24Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=... [more]
Match NameE-valueIdentityDescription
XP_022140618.11.46e-258100.00coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Mom... [more]
XP_023512859.19.64e-14762.90coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
XP_022986200.14.62e-11661.73coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Cuc... [more]
KAG7010704.14.06e-10660.44Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2, partial ... [more]
KAE8075836.16.20e-10547.03hypothetical protein FH972_014523 [Carpinus fangiana][more]
Match NameE-valueIdentityDescription
A0A6J1CIE17.09e-259100.00coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=M... [more]
A0A6J1JDF02.24e-11661.73coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=C... [more]
A0A5N6RBV53.00e-10547.03Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_014523 PE=3 SV=1[more]
A0A1Q3B9H94.30e-9844.32Transferase domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3... [more]
A0A6J1CHV41.30e-9545.56LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltr... [more]
Match NameE-valueIdentityDescription
AT1G03495.17.3e-5133.71HXXXD-type acyl-transferase family protein [more]
AT1G03940.11.2e-5033.89HXXXD-type acyl-transferase family protein [more]
AT3G29680.13.2e-4636.83HXXXD-type acyl-transferase family protein [more]
AT3G29670.15.1e-4432.72HXXXD-type acyl-transferase family protein [more]
AT5G39090.12.5e-4334.45HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 29..333
e-value: 1.3E-34
score: 119.6
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 128..341
e-value: 5.9E-51
score: 174.6
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..118
e-value: 4.1E-23
score: 84.0
NoneNo IPR availablePANTHERPTHR31625FAMILY NOT NAMEDcoord: 2..340
NoneNo IPR availablePANTHERPTHR31625:SF52COUMAROYL-COA:ANTHOCYANIDIN 3-O-GLUCOSIDE-6''-O-COUMAROYLTRANSFERASE 1-RELATEDcoord: 2..340

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0500.1MC02g0500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups