MC02g0489 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0489
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionformimidoyltransferase-cyclodeaminase-like isoform X2
LocationMC02: 4043349 .. 4045869 (-)
RNA-Seq ExpressionMC02g0489
SyntenyMC02g0489
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGACATCTTCGCTTCATCTCTCAATCTCGAAGAGACCCACCTGAAGGAAGGCTACGCCGAGGGCTATAAAGACGGTCTAGTCGCTGGCAAAGAGGAGGCTAAACAAGTAGGCCTTAAAGTTGGGTTCCAGGTCGGCGAGGAGTTGGGATTCTACAGAGGGTGTGTGGACGCGTGGAATTCTGCAATTCTGATCGACCCGGAACGGTTCTCGGTTCGGGTTCGGAAGAGTATCAAGCAGATGGAGGAGTTGGTGGAGAAATACCCACTTCAGGACCCTGAGAATGAGCAAGTTCAGGAGCTGATGGAAGGCTTGAGGCTCAAGTTCAGAGCGATTTGCGCCACTCTGGGTGTCAAATTGGAGTATAATGGCTATCCGAAATCGGCTTCTGATGGAAAAGAGATTGAGTTTTGATGTGATTTTTGTGGAGATTGTGTAATAGATAGGCTACATGGAGATTTCATAGTAGGTGATATAATGATTGGTGAAATTTGGGTGTTTTTTTAGATGATTGTTTTGCCTTATTATATCTAATAAGAACTCTCTGTTTCATCTTTTGTAGACTCCTCTCTTTGTTGCGCTGTAAAGGCTGGCTGTATTATTTCTATTACGGCATTTAGTTTGTCTATCAAAATATGAAAATAAAAGAATGTTGAAAAATGATTCAATATGCATGATGTACGAGTAATAGAAAACTTTCGCATGAAAGTTTTAAGTTGATCACGATTCATGATAATCACTTTATTTCCCTTGGTCCGACATCATAGTTTGTATAATCCGGTGCATTTCTCGCAAATATGAACTCAAGTGCAGCATTGCTTTGCTTTTCACAAAATTCTAGATTTGCTTTCAAAATTTGTTAATCCGTCTATCAACCTTGAAGAGAAATTACAGACGTGAAGTTATATCCTTTAACTACTGCTTGTAACATGTTCTTATCATTTTGTGCAGCTAGAGACCTGTAGTAAGGAGAGAAATGTCGAAATTGGTTCTTGCTTGCTGCAAGGTTTACATATCCGAAAGCCGAAACAAGGCTGCATTGGACTCAATTGAACGAGCTGCCAAGTTCTTCCATGAAGCCCCCATTATCAACAAATTCACGGATGAGGTTTATAACAGAGTTGGATATACCCTTGTTTCCAAGCTCCCATCAGAGCCATCTGTCCAGTCATGTTCCTTGAGAAGTGCAGTTGTGACCATGGTTAAGGCTGCATTTTCGGCAATTGACTTCGACTCGCATTGTGGCAGCCACCCACGACTTGGAGTTGTGGATCATATATGCTTTCATCCTTTGGCCCTTGCTTCCTTGGATGATGCAGCTATAATTGCCAGGTCTCTGGCGGCTGATATTGGATTTGGTCTTCAAGGTTTGTCTTTTTTTTCCACACCATACCATAATGCTAGATTTTTGGATGTGAACGTGAAAAGAATGCTAACTTAGGAGGCATTTCCATTGATGGGCTGAGCTGAAAAACAGTGGTATACTTTGTATTTCCCTAAACATTTTTAATGGGCTTCTTAAATGCAGTGTTCGCATGTTAAAACTCAGATATGAAGGCTTAATCTTTAACCTTTGACTTGAGTGTATATTATTCTTAAAACTCCTTGCTTTAATTTATTGAATTGGTAGTGCCTCGATGGTGTGCTATCAAACTTTAGATGCATAACAATATTGGAAAACCAAATGTCATTACATTGTCACCGTCATTTGTGATATGAGAGTTGAATATTTCTATGAGTTTCATGGCATCAGTTGCTATGTGTGTTATCCCTCCTGTCATTTATATTCTGCGATCCTGACGATCTTAACCATAGCCTGCAGTCCCGACATTTCTATACGGAGCGGCTCATGAAGAGGGGAGGAAGCTGGCCTTGATCAGAAGAGAGCTGGGTTATTTCAAGCCAAATTCTGATGGTTTCCAGTGGGCTGGAGGGCTGAAATCAGAGACATTGCCACTGAAGCCAGACAGGGGTCCAGCTGAAGCAAGTAAGGCAAAAGGGGTCGTCGTAATCGGGGCAACGAAGTGGGTCGATAATTACAATGTCCCGATCTTCTCTACCAATACCGCTGCTGTGTGTAAAATTGCAAAACAAGTGAGCGAGAGAGGAGGTGGACTGCCTTCAGTTCAAGCAATGGCCCTGGGTCATAAAGAAGGTGTAATTGAGGTGGCTTGTAATTTGCTTGAACCAAGTGAAGTGGGAGGGAAAATGGTTCAGCAGGAAGTTGAGCGGCTTGCAGAAAGTGAAGGCTTGGCTGTGGGGGAAGGATATTTCACAGACCTCTCACAAGAGGGCATAACTGAAAGGTACCTCAAATTGGTTTCTTCTGTGTAACCTGAAAGTGGCTTGAGATTTATTTATATTTGTCGTCTCATGTCTTGTGTGTCTTCTGCTTTGGATCTAATACTACCAGCACCTCACATCGTGCTCAAATTCCAATGTTTCGATCCTTCAAATTTGTGATTTTACAGATATTTCCACTGATATTGTAAATTGACATTGACTTTTCAGAGAGAGA

mRNA sequence

ATGGACGACATCTTCGCTTCATCTCTCAATCTCGAAGAGACCCACCTGAAGGAAGGCTACGCCGAGGGCTATAAAGACGGTCTAGTCGCTGGCAAAGAGGAGGCTAAACAAGTAGGCCTTAAAGTTGGGTTCCAGGTCGGCGAGGAGTTGGGATTCTACAGAGGGTGTGTGGACGCGTGGAATTCTGCAATTCTGATCGACCCGGAACGGTTCTCGGTTCGGGTTCGGAAGAGTATCAAGCAGATGGAGGAGTTGGTGGAGAAATACCCACTTCAGGACCCTGAGAATGAGCAAGTTCAGGAGCTGATGGAAGGCTTGAGGCTCAAGTTCAGAGCGATTTGCGCCACTCTGGGTGTCAAATTGGAACCTGTAGTAAGGAGAGAAATGTCGAAATTGGTTCTTGCTTGCTGCAAGGTTTACATATCCGAAAGCCGAAACAAGGCTGCATTGGACTCAATTGAACGAGCTGCCAAGTTCTTCCATGAAGCCCCCATTATCAACAAATTCACGGATGAGGTTTATAACAGAGTTGGATATACCCTTGTTTCCAAGCTCCCATCAGAGCCATCTGTCCAGTCATGTTCCTTGAGAAGTGCAGTTGTGACCATGGTTAAGGCTGCATTTTCGGCAATTGACTTCGACTCGCATTGTGGCAGCCACCCACGACTTGGAGTTGTGGATCATATATGCTTTCATCCTTTGGCCCTTGCTTCCTTGGATGATGCAGCTATAATTGCCAGGTCTCTGGCGGCTGATATTGGATTTGGTCTTCAAGTCCCGACATTTCTATACGGAGCGGCTCATGAAGAGGGGAGGAAGCTGGCCTTGATCAGAAGAGAGCTGGGTTATTTCAAGCCAAATTCTGATGGTTTCCAGTGGGCTGGAGGGCTGAAATCAGAGACATTGCCACTGAAGCCAGACAGGGGTCCAGCTGAAGCAAGTAAGGCAAAAGGGGTCGTCGTAATCGGGGCAACGAAGTGGGTCGATAATTACAATGTCCCGATCTTCTCTACCAATACCGCTGCTGTGTGTAAAATTGCAAAACAAGTGAGCGAGAGAGGAGGTGGACTGCCTTCAGTTCAAGCAATGGCCCTGGGTCATAAAGAAGGTGTAATTGAGGTGGCTTGTAATTTGCTTGAACCAAGTGAAGTGGGAGGGAAAATGGTTCAGCAGGAAGTTGAGCGGCTTGCAGAAAGTGAAGGCTTGGCTGTGGGGGAAGGATATTTCACAGACCTCTCACAAGAGGGCATAACTGAAAGGTACCTCAAATTGGTTTCTTCTGTGTAACCTGAAAGTGGCTTGAGATTTATTTATATTTGTCGTCTCATGTCTTGTGTGTCTTCTGCTTTGGATCTAATACTACCAGCACCTCACATCGTGCTCAAATTCCAATGTTTCGATCCTTCAAATTTGTGATTTTACAGATATTTCCACTGATATTGTAAATTGACATTGACTTTTCAGAGAGAGA

Coding sequence (CDS)

ATGGACGACATCTTCGCTTCATCTCTCAATCTCGAAGAGACCCACCTGAAGGAAGGCTACGCCGAGGGCTATAAAGACGGTCTAGTCGCTGGCAAAGAGGAGGCTAAACAAGTAGGCCTTAAAGTTGGGTTCCAGGTCGGCGAGGAGTTGGGATTCTACAGAGGGTGTGTGGACGCGTGGAATTCTGCAATTCTGATCGACCCGGAACGGTTCTCGGTTCGGGTTCGGAAGAGTATCAAGCAGATGGAGGAGTTGGTGGAGAAATACCCACTTCAGGACCCTGAGAATGAGCAAGTTCAGGAGCTGATGGAAGGCTTGAGGCTCAAGTTCAGAGCGATTTGCGCCACTCTGGGTGTCAAATTGGAACCTGTAGTAAGGAGAGAAATGTCGAAATTGGTTCTTGCTTGCTGCAAGGTTTACATATCCGAAAGCCGAAACAAGGCTGCATTGGACTCAATTGAACGAGCTGCCAAGTTCTTCCATGAAGCCCCCATTATCAACAAATTCACGGATGAGGTTTATAACAGAGTTGGATATACCCTTGTTTCCAAGCTCCCATCAGAGCCATCTGTCCAGTCATGTTCCTTGAGAAGTGCAGTTGTGACCATGGTTAAGGCTGCATTTTCGGCAATTGACTTCGACTCGCATTGTGGCAGCCACCCACGACTTGGAGTTGTGGATCATATATGCTTTCATCCTTTGGCCCTTGCTTCCTTGGATGATGCAGCTATAATTGCCAGGTCTCTGGCGGCTGATATTGGATTTGGTCTTCAAGTCCCGACATTTCTATACGGAGCGGCTCATGAAGAGGGGAGGAAGCTGGCCTTGATCAGAAGAGAGCTGGGTTATTTCAAGCCAAATTCTGATGGTTTCCAGTGGGCTGGAGGGCTGAAATCAGAGACATTGCCACTGAAGCCAGACAGGGGTCCAGCTGAAGCAAGTAAGGCAAAAGGGGTCGTCGTAATCGGGGCAACGAAGTGGGTCGATAATTACAATGTCCCGATCTTCTCTACCAATACCGCTGCTGTGTGTAAAATTGCAAAACAAGTGAGCGAGAGAGGAGGTGGACTGCCTTCAGTTCAAGCAATGGCCCTGGGTCATAAAGAAGGTGTAATTGAGGTGGCTTGTAATTTGCTTGAACCAAGTGAAGTGGGAGGGAAAATGGTTCAGCAGGAAGTTGAGCGGCTTGCAGAAAGTGAAGGCTTGGCTGTGGGGGAAGGATATTTCACAGACCTCTCACAAGAGGGCATAACTGAAAGGTACCTCAAATTGGTTTCTTCTGTGTAA

Protein sequence

MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAWNSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVKLEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYTLVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLDDAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSETLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSVQAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITERYLKLVSSV
Homology
BLAST of MC02g0489 vs. ExPASy Swiss-Prot
Match: Q6KZM5 (Glutamate formimidoyltransferase OS=Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828) OX=263820 GN=PTO1242 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 7.4e-13
Identity = 63/225 (28.00%), Postives = 102/225 (45.33%), Query Frame = 0

Query: 142 SESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYTLVSKLPSEPSVQSCSLRSAVV 201
           SE R+ + ++ I  + K      I++   D  +NR   T    +          +  A +
Sbjct: 16  SEGRDISKIEKIIDSIKNIEGVKILDLNVDPQHNRSVITFTCGIE--------RIIEAGI 75

Query: 202 TMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLDDAAIIARSLAADIGFGLQVPT 261
           +M+K A S ID + H G HPR G  D     P+  AS+DD  I +R+L   +G  L +P 
Sbjct: 76  SMIKTAASLIDMEKHSGLHPRFGATDVFPIIPIT-ASMDDCIIASRNLGRLVGSELNIPV 135

Query: 262 FLY--GAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSETLPLKPDRGPAEASKAKGV 321
           ++Y   A   E R L  IR +   ++   +          +T   +PD GP     A G 
Sbjct: 136 YMYSESAMVPERRNLENIRNKNVQYEELKE--------LIKTDKYRPDFGPDSLGSA-GA 195

Query: 322 VVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSVQAMA 365
           V+IGA   +  YN+ I + +     +IA  +  R GGL +++ +A
Sbjct: 196 VIIGARPALIAYNIYISTDDIKIGRRIASALRGRDGGLNTLKTLA 222

BLAST of MC02g0489 vs. ExPASy Swiss-Prot
Match: Q8CH62 (Protein LTO1 homolog OS=Mus musculus OX=10090 GN=Lto1 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.1e-11
Identity = 39/118 (33.05%), Postives = 64/118 (54.24%), Query Frame = 0

Query: 3   DIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAWNS 62
           DIF + +  +E    EGY EGY++G   G  E K+ G+  G ++G E+G YRG   AW  
Sbjct: 6   DIFDAVVMADERFHGEGYQEGYEEGSSLGIVEGKRYGMVHGAKIGSEIGCYRGFALAWKC 65

Query: 63  AILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 121
            +         R  K ++ +  L++ +P  DP  E++ E ++ +R KFR +C+ L V+
Sbjct: 66  LLHSGAGEKDSRKMKVVEALIALLQDFPYDDPTYEKLHEDLDRIRGKFRQLCSLLNVQ 123

BLAST of MC02g0489 vs. ExPASy Swiss-Prot
Match: Q8WV07 (Protein LTO1 homolog OS=Homo sapiens OX=9606 GN=LTO1 PE=1 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 1.2e-10
Identity = 39/130 (30.00%), Postives = 68/130 (52.31%), Query Frame = 0

Query: 3   DIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAWNS 62
           DIF + +  +E    EGY EGY++G   G  E +Q G   G ++G E+G Y+G   AW  
Sbjct: 6   DIFDAIVMADERFHGEGYREGYEEGSSLGVMEGRQHGTLHGAKIGSEIGCYQGFAFAWKC 65

Query: 63  AILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVKLE 122
            +         R  K ++ +  +++K+P  DP  +++ E ++ +R KF+  C+ L V+ +
Sbjct: 66  LLHSCTTEKDSRKMKVLESLIGMIQKFPYDDPTYDKLHEDLDKIRGKFKQFCSLLNVQPD 125

Query: 123 PVVRREMSKL 133
             +  E S L
Sbjct: 126 FKISAEGSGL 135

BLAST of MC02g0489 vs. ExPASy Swiss-Prot
Match: Q75JW3 (Protein LTO1 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0272178 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.5e-08
Identity = 36/128 (28.12%), Postives = 65/128 (50.78%), Query Frame = 0

Query: 5   FASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAWNSAI 64
           F   L++E         +G  DG   G  E  Q+G + G ++G+E+G+Y+ CV  WN  +
Sbjct: 4   FDQLLSVESDAYISSKEQGIDDGKRLGYVEGYQLGFEKGIELGQEIGYYQSCVTVWNHLV 63

Query: 65  LIDPE------------RFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRA 121
            I+              +FSVR  ++++++ +L+E Y L D  +E +   +  +RLKF+ 
Sbjct: 64  SINNNNNNNNNNNKNNLKFSVRGIQNLEKLTKLLEDYHL-DFNDENIMNTLSEIRLKFKL 123

BLAST of MC02g0489 vs. NCBI nr
Match: XP_022140379.1 (formimidoyltransferase-cyclodeaminase-like isoform X2 [Momordica charantia])

HSP 1 Score: 834 bits (2155), Expect = 9.89e-305
Identity = 426/428 (99.53%), Postives = 426/428 (99.53%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW
Sbjct: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK
Sbjct: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120

Query: 121 LEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180
           LE  VRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT
Sbjct: 121 LEYNVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180

Query: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240
           LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD
Sbjct: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240

Query: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300
           DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE
Sbjct: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300

Query: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360
           TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV
Sbjct: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360

Query: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420
           QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER
Sbjct: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420

Query: 421 YLKLVSSV 428
           YLKLVSSV
Sbjct: 421 YLKLVSSV 428

BLAST of MC02g0489 vs. NCBI nr
Match: XP_008448205.1 (PREDICTED: formimidoyltransferase-cyclodeaminase isoform X2 [Cucumis melo])

HSP 1 Score: 723 bits (1867), Expect = 7.25e-261
Identity = 364/426 (85.45%), Postives = 397/426 (93.19%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIF SSLNLEE HLKEGYA+GY+DGLVAGKEEA+QVGLKVGF+VGEE+GFYRGCVD W
Sbjct: 1   MDDIFDSSLNLEEAHLKEGYADGYEDGLVAGKEEAEQVGLKVGFEVGEEIGFYRGCVDVW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NSAI I+PERFSVRVRKS+K +EEL+EKYPLQDPENEQVQELMEGLRLKFRAI ATLGVK
Sbjct: 61  NSAIRIEPERFSVRVRKSVKLLEELLEKYPLQDPENEQVQELMEGLRLKFRAISATLGVK 120

Query: 121 LEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180
           LE  V+R+MSKL+LACCKVYISESRNKAAL+SIERA K F +APIINKFTDEVYNRVGYT
Sbjct: 121 LEYNVKRKMSKLILACCKVYISESRNKAALESIERATKLFPDAPIINKFTDEVYNRVGYT 180

Query: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240
           LVSKLPS+PS +SCSL SAV+ MVKAAFSAIDF+SHCGSHPRLGVVDHICFHPLA ASL+
Sbjct: 181 LVSKLPSQPSGKSCSLISAVLNMVKAAFSAIDFNSHCGSHPRLGVVDHICFHPLASASLE 240

Query: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300
           DAA+IA+ LAAD+G+ LQVPTFLYGAAHEEGRKL+ IRRELGYFKPNS+G +WAGGLKS+
Sbjct: 241 DAALIAKYLAADVGYRLQVPTFLYGAAHEEGRKLSEIRRELGYFKPNSEGLKWAGGLKSD 300

Query: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360
           +LPLKPD GPAEASKAKGVVVIGATKWVDNYNVP+FSTN +AV KIAKQVSERGGGL SV
Sbjct: 301 SLPLKPDDGPAEASKAKGVVVIGATKWVDNYNVPVFSTNISAVRKIAKQVSERGGGLSSV 360

Query: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420
           QAMAL H EGVIEVACNLLEPS+VGGK+VQQEVERLAE+EGL VGEGYFTDLSQE I ER
Sbjct: 361 QAMALAHDEGVIEVACNLLEPSKVGGKVVQQEVERLAENEGLGVGEGYFTDLSQENIIER 420

Query: 421 YLKLVS 426
           YLKL S
Sbjct: 421 YLKLFS 426

BLAST of MC02g0489 vs. NCBI nr
Match: XP_038902357.1 (formimidoyltransferase-cyclodeaminase-like [Benincasa hispida])

HSP 1 Score: 723 bits (1866), Expect = 1.94e-260
Identity = 370/443 (83.52%), Postives = 397/443 (89.62%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIF SSLNLEETHLKEG+AEGYKDGLVAGKEEA+QVGLKVGF+VGEELGFYRGCVD W
Sbjct: 1   MDDIFDSSLNLEETHLKEGFAEGYKDGLVAGKEEAEQVGLKVGFEVGEELGFYRGCVDVW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NS I I+PERFS+RVRKS+KQMEELVEKYPLQDPENEQVQELMEGLRLKFRAI ATLGVK
Sbjct: 61  NSVIRIEPERFSIRVRKSVKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAISATLGVK 120

Query: 121 LE-----------------PVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEA 180
           LE                  V R++MSKLVLACCKVYISESRNKA L+SIE+AAK F +A
Sbjct: 121 LEYTGYPKSTSDGKDIEFPAVKRKKMSKLVLACCKVYISESRNKAGLESIEQAAKLFPDA 180

Query: 181 PIINKFTDEVYNRVGYTLVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRL 240
           PIINKFTDEVYNRVGYTLVSKLPS+PS +SCSLRSAV+ MVKAAFSAID + HCGSHPRL
Sbjct: 181 PIINKFTDEVYNRVGYTLVSKLPSQPSGKSCSLRSAVLNMVKAAFSAIDLNLHCGSHPRL 240

Query: 241 GVVDHICFHPLALASLDDAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGY 300
           GVVDHICFHPLA ASLDDAA+IA+SLAADIG GLQVPTFLYGAAHEEGRKLA+IRRELGY
Sbjct: 241 GVVDHICFHPLASASLDDAAVIAKSLAADIGCGLQVPTFLYGAAHEEGRKLAMIRRELGY 300

Query: 301 FKPNSDGFQWAGGLKSETLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAV 360
           FKPNSDG  WAGGLKS++LPL+PD GPAEASKAKGVVVIGATKWVDNYNVPIFSTN  A+
Sbjct: 301 FKPNSDGLLWAGGLKSDSLPLRPDEGPAEASKAKGVVVIGATKWVDNYNVPIFSTNIGAI 360

Query: 361 CKIAKQVSERGGGLPSVQAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLA 420
            KIAKQVSERGGGL SVQAMAL H EGVIEVACNLLE ++VGGKMVQQEVERLAE+EGL 
Sbjct: 361 RKIAKQVSERGGGLSSVQAMALAHDEGVIEVACNLLESNKVGGKMVQQEVERLAENEGLG 420

Query: 421 VGEGYFTDLSQEGITERYLKLVS 426
           VG+GYFTDLSQE I ERYLKL+S
Sbjct: 421 VGKGYFTDLSQESIIERYLKLLS 443

BLAST of MC02g0489 vs. NCBI nr
Match: XP_004139960.1 (formimidoyltransferase-cyclodeaminase isoform X2 [Cucumis sativus])

HSP 1 Score: 720 bits (1858), Expect = 1.70e-259
Identity = 363/426 (85.21%), Postives = 394/426 (92.49%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIF SSLNLEE HLKEGYA+GYKDGLVAGKEEA+QVGLKVGF+VGEELGFYRGCVD W
Sbjct: 1   MDDIFDSSLNLEEAHLKEGYADGYKDGLVAGKEEAEQVGLKVGFEVGEELGFYRGCVDVW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NS I I+PERFS+RVRKS+K MEEL+EKYPLQDPENEQVQELMEGLRLKFRA+ ATLGVK
Sbjct: 61  NSVIRIEPERFSIRVRKSVKLMEELLEKYPLQDPENEQVQELMEGLRLKFRAVSATLGVK 120

Query: 121 LEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180
           LE  V+R+MSKL LACCKVYISESRNKAAL+SIERA K F +APIINKFTDEVYNRVGYT
Sbjct: 121 LEYHVKRKMSKLFLACCKVYISESRNKAALESIERATKLFPDAPIINKFTDEVYNRVGYT 180

Query: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240
           LVSKLPS  S +SCSL SAV+ MVKAAFSAIDF+SHCGSHPRLGVVDHICFHPLA A+L+
Sbjct: 181 LVSKLPSHLSGKSCSLISAVLNMVKAAFSAIDFNSHCGSHPRLGVVDHICFHPLASATLE 240

Query: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300
           DAA+IA+ LAAD+G+ LQVPTFLYGAAHEEGRKLA+IRRELGYFKPNS+G +WAGGLKS+
Sbjct: 241 DAALIAKYLAADVGYSLQVPTFLYGAAHEEGRKLAVIRRELGYFKPNSEGSKWAGGLKSD 300

Query: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360
           +LPLKPD GPAEASKAKGVVVIGATKWVDNYNVP+FSTN +AV KIAKQVSERGGGL SV
Sbjct: 301 SLPLKPDDGPAEASKAKGVVVIGATKWVDNYNVPVFSTNISAVRKIAKQVSERGGGLSSV 360

Query: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420
           QAMAL H EGVIEVACNLLEPS+VGGKMVQQEVERLAE+EGL VGEGYFTDLSQE I ER
Sbjct: 361 QAMALAHDEGVIEVACNLLEPSKVGGKMVQQEVERLAENEGLGVGEGYFTDLSQESIIER 420

Query: 421 YLKLVS 426
           YL+L S
Sbjct: 421 YLELFS 426

BLAST of MC02g0489 vs. NCBI nr
Match: KGN46702.2 (hypothetical protein Csa_020611 [Cucumis sativus])

HSP 1 Score: 663 bits (1710), Expect = 4.18e-237
Identity = 340/420 (80.95%), Postives = 367/420 (87.38%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIF SSLNLEE HLKEGYA+GYKDGLVAGKEEA+QVGLKVGF+VGEELGFYRGCVD W
Sbjct: 1   MDDIFDSSLNLEEAHLKEGYADGYKDGLVAGKEEAEQVGLKVGFEVGEELGFYRGCVDVW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NS I I+PERFS+RVRKS+K MEEL+EKYPLQDPENEQVQELMEGLRLKFRA        
Sbjct: 61  NSVIRIEPERFSIRVRKSVKLMEELLEKYPLQDPENEQVQELMEGLRLKFRA-------- 120

Query: 121 LEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180
                             VYISESRNKAAL+SIERA K F +APIINKFTDEVYNRVGYT
Sbjct: 121 ------------------VYISESRNKAALESIERATKLFPDAPIINKFTDEVYNRVGYT 180

Query: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240
           LVSKLPS  S +SCSL SAV+ MVKAAFSAIDF+SHCGSHPRLGVVDHICFHPLA A+L+
Sbjct: 181 LVSKLPSHLSGKSCSLISAVLNMVKAAFSAIDFNSHCGSHPRLGVVDHICFHPLASATLE 240

Query: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300
           DAA+IA+ LAAD+G+ LQVPTFLYGAAHEEGRKLA+IRRELGYFKPNS+G +WAGGLKS+
Sbjct: 241 DAALIAKYLAADVGYSLQVPTFLYGAAHEEGRKLAVIRRELGYFKPNSEGSKWAGGLKSD 300

Query: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360
           +LPLKPD GPAEASKAKGVVVIGATKWVDNYNVP+FSTN +AV KIAKQVSERGGGL SV
Sbjct: 301 SLPLKPDDGPAEASKAKGVVVIGATKWVDNYNVPVFSTNISAVRKIAKQVSERGGGLSSV 360

Query: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420
           QAMAL H EGVIEVACNLLEPS+VGGKMVQQEVERLAE+EGL VGEGYFTDLSQE I ER
Sbjct: 361 QAMALAHDEGVIEVACNLLEPSKVGGKMVQQEVERLAENEGLGVGEGYFTDLSQESIIER 394

BLAST of MC02g0489 vs. ExPASy TrEMBL
Match: A0A6J1CFK0 (formimidoyltransferase-cyclodeaminase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011067 PE=4 SV=1)

HSP 1 Score: 834 bits (2155), Expect = 4.79e-305
Identity = 426/428 (99.53%), Postives = 426/428 (99.53%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW
Sbjct: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK
Sbjct: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120

Query: 121 LEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180
           LE  VRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT
Sbjct: 121 LEYNVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180

Query: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240
           LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD
Sbjct: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240

Query: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300
           DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE
Sbjct: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300

Query: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360
           TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV
Sbjct: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360

Query: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420
           QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER
Sbjct: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420

Query: 421 YLKLVSSV 428
           YLKLVSSV
Sbjct: 421 YLKLVSSV 428

BLAST of MC02g0489 vs. ExPASy TrEMBL
Match: A0A1S3BJ53 (formimidoyltransferase-cyclodeaminase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490466 PE=4 SV=1)

HSP 1 Score: 723 bits (1867), Expect = 3.51e-261
Identity = 364/426 (85.45%), Postives = 397/426 (93.19%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIF SSLNLEE HLKEGYA+GY+DGLVAGKEEA+QVGLKVGF+VGEE+GFYRGCVD W
Sbjct: 1   MDDIFDSSLNLEEAHLKEGYADGYEDGLVAGKEEAEQVGLKVGFEVGEEIGFYRGCVDVW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NSAI I+PERFSVRVRKS+K +EEL+EKYPLQDPENEQVQELMEGLRLKFRAI ATLGVK
Sbjct: 61  NSAIRIEPERFSVRVRKSVKLLEELLEKYPLQDPENEQVQELMEGLRLKFRAISATLGVK 120

Query: 121 LEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180
           LE  V+R+MSKL+LACCKVYISESRNKAAL+SIERA K F +APIINKFTDEVYNRVGYT
Sbjct: 121 LEYNVKRKMSKLILACCKVYISESRNKAALESIERATKLFPDAPIINKFTDEVYNRVGYT 180

Query: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240
           LVSKLPS+PS +SCSL SAV+ MVKAAFSAIDF+SHCGSHPRLGVVDHICFHPLA ASL+
Sbjct: 181 LVSKLPSQPSGKSCSLISAVLNMVKAAFSAIDFNSHCGSHPRLGVVDHICFHPLASASLE 240

Query: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300
           DAA+IA+ LAAD+G+ LQVPTFLYGAAHEEGRKL+ IRRELGYFKPNS+G +WAGGLKS+
Sbjct: 241 DAALIAKYLAADVGYRLQVPTFLYGAAHEEGRKLSEIRRELGYFKPNSEGLKWAGGLKSD 300

Query: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360
           +LPLKPD GPAEASKAKGVVVIGATKWVDNYNVP+FSTN +AV KIAKQVSERGGGL SV
Sbjct: 301 SLPLKPDDGPAEASKAKGVVVIGATKWVDNYNVPVFSTNISAVRKIAKQVSERGGGLSSV 360

Query: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420
           QAMAL H EGVIEVACNLLEPS+VGGK+VQQEVERLAE+EGL VGEGYFTDLSQE I ER
Sbjct: 361 QAMALAHDEGVIEVACNLLEPSKVGGKVVQQEVERLAENEGLGVGEGYFTDLSQENIIER 420

Query: 421 YLKLVS 426
           YLKL S
Sbjct: 421 YLKLFS 426

BLAST of MC02g0489 vs. ExPASy TrEMBL
Match: A0A2C9US66 (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_13G150900 PE=4 SV=1)

HSP 1 Score: 613 bits (1582), Expect = 1.27e-217
Identity = 313/428 (73.13%), Postives = 354/428 (82.71%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           + DIF SSLNLE+TH KEGY EGY  GL+AGKEEA+QVGLK GF+VGEELGFYRGCVD W
Sbjct: 12  IQDIFESSLNLEDTHYKEGYDEGYSHGLLAGKEEARQVGLKTGFEVGEELGFYRGCVDVW 71

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NSAI IDP  FS RV+KSIKQMEEL+EKYP  DPE+E V+E+M+ LRLKFR I A LGVK
Sbjct: 72  NSAIRIDPTAFSTRVQKSIKQMEELIEKYPRLDPEDESVEEIMDSLRLKFRVIRAGLGVK 131

Query: 121 LEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 180
           LE   R +M K  LACCKVYISESRNKAAL+SIE AAK F +API+NKF D  YNRVGYT
Sbjct: 132 LEYDGREDMLKWTLACCKVYISESRNKAALESIEHAAKLFPQAPIVNKFEDATYNRVGYT 191

Query: 181 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 240
           LVS L  +PS+ SC L+SAV+ MVK+AF AID ++HCGSHPRLGVVDHICFHPL  ASL+
Sbjct: 192 LVSSLTPKPSLDSCPLKSAVLAMVKSAFEAIDLEAHCGSHPRLGVVDHICFHPLEHASLN 251

Query: 241 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 300
            AA IA+SLAAD+G  LQVPTFLYGAA+E+GR L  IRRELGYFKPN  G QW GG KSE
Sbjct: 252 QAAGIAKSLAADVGSSLQVPTFLYGAANEQGRTLDSIRRELGYFKPNY-GHQWTGGPKSE 311

Query: 301 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 360
            LP+KPD GPA+ ++AKGVVVIGAT+WVDNYNVP+ ST+ AAV KIAK+VS RGGGL SV
Sbjct: 312 LLPMKPDEGPAQVTRAKGVVVIGATQWVDNYNVPVLSTDIAAVRKIAKKVSGRGGGLSSV 371

Query: 361 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 420
           Q MAL H + VIEVACNLLEPS+VGG+ VQQEVERLAE EG+AVG+GYFTD SQE I E 
Sbjct: 372 QTMALAHGDDVIEVACNLLEPSKVGGERVQQEVERLAEEEGIAVGKGYFTDFSQEKIIES 431

Query: 421 YLKLVSSV 428
           YLKL S +
Sbjct: 432 YLKLSSQL 438

BLAST of MC02g0489 vs. ExPASy TrEMBL
Match: A0A7J6EXW9 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_001825 PE=4 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 1.19e-212
Identity = 306/437 (70.02%), Postives = 353/437 (80.78%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIF SSLNLEE HLKEG+ EGYKDGL +GKE+ KQVGLK GF+VGEELGFY+GCVD W
Sbjct: 1   MDDIFDSSLNLEEAHLKEGFDEGYKDGLGSGKEDGKQVGLKTGFEVGEELGFYKGCVDIW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NSAI +DP +FS RV+K IKQMEEL+EKYP+ +PENE V E+M+ LRLKFRA+CA++GVK
Sbjct: 61  NSAIHVDPTQFSSRVQKGIKQMEELIEKYPIMEPENESVHEIMDSLRLKFRAVCASMGVK 120

Query: 121 LE-------PVVRRE--MSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTD 180
           LE         V +E  M K +L CCKVYISESRN+AAL+SIERAAK F +APIINKF D
Sbjct: 121 LEYNGYPKSSSVEKEKIMLKSMLGCCKVYISESRNRAALESIERAAKLFPKAPIINKFVD 180

Query: 181 EVYNRVGYTLVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICF 240
           E YNRVGYTLVSKL  +PS   C LR++V+ MVKAA  +ID + H GSHPRLG+VDHICF
Sbjct: 181 ETYNRVGYTLVSKLDPQPSSDPCPLRTSVLAMVKAALESIDLELHSGSHPRLGIVDHICF 240

Query: 241 HPLALASLDDAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGF 300
           HPL   SLD AA IA+SLAAD+G+GLQVPTFLYGAAH EGR L  IRRELGYFKPNS G 
Sbjct: 241 HPLLSTSLDQAANIAKSLAADVGYGLQVPTFLYGAAHGEGRTLDTIRRELGYFKPNSSGN 300

Query: 301 QWAGGLKSETLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVS 360
           QW GGLK E+L LKPD GP      KGV+VIGAT+WVDNYNVP+FST+ +AV +IAK VS
Sbjct: 301 QWVGGLKQESLALKPDEGPPHVIPTKGVIVIGATRWVDNYNVPVFSTDLSAVRRIAKGVS 360

Query: 361 ERGGGLPSVQAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTD 420
            RGGGLPSVQAMAL H E VIEVACNLL+P+ V G  VQ EVERLA+ EG++VG+GYFTD
Sbjct: 361 GRGGGLPSVQAMALAHGETVIEVACNLLDPNNVRGDRVQLEVERLAKEEGISVGKGYFTD 420

Query: 421 LSQEGITERYLKLVSSV 428
           LSQE I + YLKL S++
Sbjct: 421 LSQEKIIQNYLKLDSAL 437

BLAST of MC02g0489 vs. ExPASy TrEMBL
Match: A0A7J6H820 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_005504 PE=4 SV=1)

HSP 1 Score: 605 bits (1560), Expect = 1.63e-212
Identity = 306/437 (70.02%), Postives = 353/437 (80.78%), Query Frame = 0

Query: 1   MDDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAW 60
           MDDIF SSLNLEE HLKEG+ EGYKDGL +GKE+ KQVGLK GF+VGEELGFY+GCVD W
Sbjct: 1   MDDIFDSSLNLEEAHLKEGFDEGYKDGLGSGKEDGKQVGLKTGFEVGEELGFYKGCVDIW 60

Query: 61  NSAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGVK 120
           NSAI +DP +FS RV+K IKQMEEL+EKYP+ +PENE V E+M+ LRLKFRA+CA++GVK
Sbjct: 61  NSAIHVDPTQFSSRVQKGIKQMEELIEKYPIMEPENESVHEIMDSLRLKFRAVCASMGVK 120

Query: 121 LE-------PVVRRE--MSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTD 180
           LE         V +E  M K +L CCKVYISESRN+AAL+SIERAAK F +APIINKF D
Sbjct: 121 LEYNGYPKSSSVEKEKIMLKSMLGCCKVYISESRNRAALESIERAAKLFPKAPIINKFVD 180

Query: 181 EVYNRVGYTLVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICF 240
           E YNRVGYTLVSKL  +PS   C LR++V+ MVKAA  +ID + H GSHPRLG+VDHICF
Sbjct: 181 ETYNRVGYTLVSKLDPQPSSDPCPLRTSVLAMVKAALESIDLELHSGSHPRLGIVDHICF 240

Query: 241 HPLALASLDDAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGF 300
           HPL   SLD AA IA+SLAAD+G+GLQVPTFLYGAAH EGR L  IRRELGYFKPNS G 
Sbjct: 241 HPLLSTSLDQAANIAKSLAADVGYGLQVPTFLYGAAHGEGRTLDTIRRELGYFKPNSSGN 300

Query: 301 QWAGGLKSETLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVS 360
           QW GGLK E+L LKPD GP      KGV+VIGAT+WVDNYNVP+FST+ +AV +IAK VS
Sbjct: 301 QWVGGLKQESLALKPDEGPPHVIPTKGVIVIGATRWVDNYNVPVFSTDLSAVRRIAKGVS 360

Query: 361 ERGGGLPSVQAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTD 420
            RGGGLPSVQAMAL H E VIEVACNLL+P+ V G  VQ EVERLA+ EG++VG+GYFTD
Sbjct: 361 GRGGGLPSVQAMALAHGETVIEVACNLLDPNNVRGDRVQLEVERLAKEEGISVGKGYFTD 420

Query: 421 LSQEGITERYLKLVSSV 428
           LSQE I + YLKL S++
Sbjct: 421 LSQEKIIQNYLKLDSAL 437

BLAST of MC02g0489 vs. TAIR 10
Match: AT2G20830.2 (transferases;folic acid binding )

HSP 1 Score: 467.2 bits (1201), Expect = 1.4e-131
Identity = 236/427 (55.27%), Postives = 304/427 (71.19%), Query Frame = 0

Query: 2   DDIFASSLNLEETHLKEGYAEGYKDGLVAGKEEAKQVGLKVGFQVGEELGFYRGCVDAWN 61
           +D     + LEETH+++G+ EGY++GLV+G+E+A+ +GLK+GF+ GE +GFYRGC   WN
Sbjct: 7   EDFLDCIVRLEETHVQQGFDEGYEEGLVSGREDARHLGLKLGFETGELIGFYRGCSALWN 66

Query: 62  SAILIDPERFSVRVRKSIKQMEELVEKYPLQDPENEQVQELMEGLRLKFRAICATLGV-K 121
           SA+ IDP RFS ++ K +     L++K PL DPE+E    + + LR+KF  ICA+LG  K
Sbjct: 67  SALRIDPTRFSPQLHKHLNDFHVLLDKIPLLDPEDEAKDGIKDDLRVKFSIICASLGFSK 126

Query: 122 LEPVVRREMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYT 181
            +     EM + +L CCKVYISE+RNK AL++IERA K F  A I+NKF D  Y RVGYT
Sbjct: 127 KQFEWSEEMLREMLGCCKVYISEARNKTALEAIERALKPFPPAAIVNKFEDAAYGRVGYT 186

Query: 182 LVSKLPSEPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLD 241
           +VS L +     S SL++AV  MVK A   I+ + HCGSHPRLGVVDHICFHPL+  S++
Sbjct: 187 VVSSLANG---SSSSLKNAVFAMVKTALDTINLELHCGSHPRLGVVDHICFHPLSQTSIE 246

Query: 242 DAAIIARSLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSE 301
             + +A SLA DIG  L+VPT+LYGAA +E   L  IRR+LGYFK N +G +WAGG   E
Sbjct: 247 QVSSVANSLAMDIGSILRVPTYLYGAAEKEQCTLDSIRRKLGYFKANREGHEWAGGFDLE 306

Query: 302 TLPLKPDRGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSV 361
            +PLKPD GP E SKAKGVV +GA  WV NYNVP+ S +  AV +IA++ SERGGGL SV
Sbjct: 307 MVPLKPDAGPQEVSKAKGVVAVGACGWVSNYNVPVMSNDLKAVRRIARKTSERGGGLASV 366

Query: 362 QAMALGHKEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITER 421
           Q MAL H EGVIEVACNLL PS+VGG  VQ  +ERL   EGL VG+GY+TD + + I ER
Sbjct: 367 QTMALVHGEGVIEVACNLLNPSQVGGDEVQGLIERLGREEGLLVGKGYYTDYTPDQIVER 426

Query: 422 YLKLVSS 428
           Y+ L+++
Sbjct: 427 YMDLLNN 430

BLAST of MC02g0489 vs. TAIR 10
Match: AT2G20830.3 (transferases;folic acid binding )

HSP 1 Score: 352.1 bits (902), Expect = 6.5e-97
Identity = 178/300 (59.33%), Postives = 218/300 (72.67%), Query Frame = 0

Query: 128 EMSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYTLVSKLPS 187
           EM + +L CCKVYISE+RNK AL++IERA K F  A I+NKF D  Y RVGYT+VS L +
Sbjct: 44  EMLREMLGCCKVYISEARNKTALEAIERALKPFPPAAIVNKFEDAAYGRVGYTVVSSLAN 103

Query: 188 EPSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLDDAAIIAR 247
                S SL++AV  MVK A   I+ + HCGSHPRLGVVDHICFHPL+  S++  + +A 
Sbjct: 104 G---SSSSLKNAVFAMVKTALDTINLELHCGSHPRLGVVDHICFHPLSQTSIEQVSSVAN 163

Query: 248 SLAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSETLPLKPD 307
           SLA DIG  L+VPT+LYGAA +E   L  IRR+LGYFK N +G +WAGG   E +PLKPD
Sbjct: 164 SLAMDIGSILRVPTYLYGAAEKEQCTLDSIRRKLGYFKANREGHEWAGGFDLEMVPLKPD 223

Query: 308 RGPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSVQAMALGH 367
            GP E SKAKGVV +GA  WV NYNVP+ S +  AV +IA++ SERGGGL SVQ MAL H
Sbjct: 224 AGPQEVSKAKGVVAVGACGWVSNYNVPVMSNDLKAVRRIARKTSERGGGLASVQTMALVH 283

Query: 368 KEGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITERYLKLVSS 427
            EGVIEVACNLL PS+VGG  VQ  +ERL   EGL VG+GY+TD + + I ERY+ L+++
Sbjct: 284 GEGVIEVACNLLNPSQVGGDEVQGLIERLGREEGLLVGKGYYTDYTPDQIVERYMDLLNN 340

BLAST of MC02g0489 vs. TAIR 10
Match: AT2G20830.1 (transferases;folic acid binding )

HSP 1 Score: 349.7 bits (896), Expect = 3.2e-96
Identity = 177/299 (59.20%), Postives = 217/299 (72.58%), Query Frame = 0

Query: 129 MSKLVLACCKVYISESRNKAALDSIERAAKFFHEAPIINKFTDEVYNRVGYTLVSKLPSE 188
           M + +L CCKVYISE+RNK AL++IERA K F  A I+NKF D  Y RVGYT+VS L + 
Sbjct: 1   MLREMLGCCKVYISEARNKTALEAIERALKPFPPAAIVNKFEDAAYGRVGYTVVSSLANG 60

Query: 189 PSVQSCSLRSAVVTMVKAAFSAIDFDSHCGSHPRLGVVDHICFHPLALASLDDAAIIARS 248
               S SL++AV  MVK A   I+ + HCGSHPRLGVVDHICFHPL+  S++  + +A S
Sbjct: 61  ---SSSSLKNAVFAMVKTALDTINLELHCGSHPRLGVVDHICFHPLSQTSIEQVSSVANS 120

Query: 249 LAADIGFGLQVPTFLYGAAHEEGRKLALIRRELGYFKPNSDGFQWAGGLKSETLPLKPDR 308
           LA DIG  L+VPT+LYGAA +E   L  IRR+LGYFK N +G +WAGG   E +PLKPD 
Sbjct: 121 LAMDIGSILRVPTYLYGAAEKEQCTLDSIRRKLGYFKANREGHEWAGGFDLEMVPLKPDA 180

Query: 309 GPAEASKAKGVVVIGATKWVDNYNVPIFSTNTAAVCKIAKQVSERGGGLPSVQAMALGHK 368
           GP E SKAKGVV +GA  WV NYNVP+ S +  AV +IA++ SERGGGL SVQ MAL H 
Sbjct: 181 GPQEVSKAKGVVAVGACGWVSNYNVPVMSNDLKAVRRIARKTSERGGGLASVQTMALVHG 240

Query: 369 EGVIEVACNLLEPSEVGGKMVQQEVERLAESEGLAVGEGYFTDLSQEGITERYLKLVSS 428
           EGVIEVACNLL PS+VGG  VQ  +ERL   EGL VG+GY+TD + + I ERY+ L+++
Sbjct: 241 EGVIEVACNLLNPSQVGGDEVQGLIERLGREEGLLVGKGYYTDYTPDQIVERYMDLLNN 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6KZM57.4e-1328.00Glutamate formimidoyltransferase OS=Picrophilus torridus (strain ATCC 700027 / D... [more]
Q8CH621.1e-1133.05Protein LTO1 homolog OS=Mus musculus OX=10090 GN=Lto1 PE=1 SV=1[more]
Q8WV071.2e-1030.00Protein LTO1 homolog OS=Homo sapiens OX=9606 GN=LTO1 PE=1 SV=2[more]
Q75JW32.5e-0828.13Protein LTO1 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0272178 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022140379.19.89e-30599.53formimidoyltransferase-cyclodeaminase-like isoform X2 [Momordica charantia][more]
XP_008448205.17.25e-26185.45PREDICTED: formimidoyltransferase-cyclodeaminase isoform X2 [Cucumis melo][more]
XP_038902357.11.94e-26083.52formimidoyltransferase-cyclodeaminase-like [Benincasa hispida][more]
XP_004139960.11.70e-25985.21formimidoyltransferase-cyclodeaminase isoform X2 [Cucumis sativus][more]
KGN46702.24.18e-23780.95hypothetical protein Csa_020611 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1CFK04.79e-30599.53formimidoyltransferase-cyclodeaminase-like isoform X2 OS=Momordica charantia OX=... [more]
A0A1S3BJ533.51e-26185.45formimidoyltransferase-cyclodeaminase isoform X2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A2C9US661.27e-21773.13Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_13G150900 PE=4 SV=... [more]
A0A7J6EXW91.19e-21270.02Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_001825 PE=4 SV=1[more]
A0A7J6H8201.63e-21270.02Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_005504 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G20830.21.4e-13155.27transferases;folic acid binding [more]
AT2G20830.36.5e-9759.33transferases;folic acid binding [more]
AT2G20830.13.2e-9659.20transferases;folic acid binding [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013802Formiminotransferase, C-terminal subdomainSMARTSM01221FTCD_2coord: 331..421
e-value: 0.0067
score: -23.0
IPR012886Formiminotransferase, N-terminal subdomainSMARTSM01222FTCD_N_2coord: 133..326
e-value: 2.5E-85
score: 299.4
IPR012886Formiminotransferase, N-terminal subdomainPFAMPF07837FTCD_Ncoord: 134..325
e-value: 6.5E-52
score: 175.8
IPR019191Essential protein Yae1, N-terminalPFAMPF09811Yae1_Ncoord: 19..57
e-value: 2.7E-9
score: 36.6
IPR037064Formiminotransferase, N-terminal subdomain superfamilyGENE3D3.30.990.10coord: 132..327
e-value: 5.2E-59
score: 200.9
IPR037070Formiminotransferase, C-terminal subdomain superfamilyGENE3D3.30.70.670coord: 330..423
e-value: 2.9E-10
score: 42.4
NoneNo IPR availablePANTHERPTHR12234:SF1FORMIMINOTRANSFERASE N-TERMINAL SUBDOMAIN-CONTAINING PROTEINcoord: 130..424
NoneNo IPR availablePANTHERPTHR12234FORMIMINOTRANSFERASE-CYCLODEAMINASEcoord: 130..424
IPR022384Formiminotransferase catalytic domain superfamilySUPERFAMILY55116Formiminotransferase domain of formiminotransferase-cyclodeaminase.coord: 134..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0489.1MC02g0489.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0034220 ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005542 folic acid binding
molecular_function GO:0008381 mechanosensitive ion channel activity
molecular_function GO:0016740 transferase activity