MC02g0458 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0458
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNEDD8-activating enzyme E1 regulatory subunit
LocationMC02: 3845544 .. 3854130 (-)
RNA-Seq ExpressionMC02g0458
SyntenyMC02g0458
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGATCTGGGGTGAACAAGGACAGGCAGCCCTTGAAAAAGCGAGTATATGCCTTCTCAACTGTGGCCCTACAGGTTCCGAGACTTTGAAAAATCTTGTTCTTGGAGGGATTGGAAGTATCACCATAGTTGACGGTACCAAGGTAGAAGAGGGCGATCTTGGCAACAACTTCATGGGTAAGTAATACGTATTTGTCATTGGCAATTGCCTATGGACCTATGAATAATATATTGACATAATTTAAGTAGCTCAATCAATCTGAAGATAAATCAATCATTCAGGACCACTTCCAATTAGCATTCAGGAGTTACCTTTTCAAAATAACGTTTTGAGAAATCATTGTCAACAATAAACAATCCTTTTTCAAATTCTGCAATCCATATATTATATATATTTGAACAATCTGCAGAATATTAATAAATACTTTTGAGCTATAACTACTTCTGTAACGGAAAAACAACACATAGTTTATCAACTAAAACATTTTCTTGTTTCATATCATTATTTTTCTAGTGGATGAATCAAGTATCGGACAGTCAAAAGCAAAGTGTGTATGTGCATTTCTTCAAGAGCTAAATGATGCTGTAAAAGCTAAGTTTATAGAAGAGTTTCCAGAGGCGTTAATTGAAACAAGACCATCATTCTTTTCTCAGTTTACGTTAGTAGTTGCTACACAGGTAATGTGATATTCATACTTGTTAATTTATGTGGCACCTCTTTACTAGATTTCTTGGTTTTTCTCTCACCTCTGTCATCTCTTATATTACCCCATCACGTCCTTGGTGCAATGGTTTTTGATATCTCTAGGATTGTTGACATAAATGAAATTGTTTCTTATAGAAAAATTATAAAAAATCAGGATTGTTTCATATTCTTTTTAGTTACCATAGTAGAAATGTATATTCTTTTTTACAAGAAACACAAATTTTGGTAGAGTACTCAAAAGCCTGGGTTTTGTTAGATTGTGAGTTTTTTGGATACTAGTTCTGTAATATGTTATAGTATGGCTATAAAAAAAAAATGTTATAGTATGGAAACTTTTTTCTAAGGTTTTGTTGGGGATAGAGTGAAGATTTACTTGTATGTGCTATGTGGTTTATAGATTTGTTGTTTTGTTGAAACTTATGTAACTTTTCACTATCTTAATTGCACTTTGTAACGGATGCTTTGAATTTATTCAGTTGGGTGAGGAGTGGATTGTAAAGCTTGACAGAATCTGTAGGAAGGCAAATGTCATATTGATTGTTGCACGCTCCTACGGCCTTACTGGGCTTGTCAGAATCAGTTTAAAGGTAATGTATTTACTAATGGTACTTTAGTTGTCCAACCTTAAACAAACTATGATCAATGGGAAAACTTATATTAGTTACACAGAAAAAACTATGTGGTATGCATAGAATGGATCAAAATATTTATGTTACCTCCTTTACGAGAAAAAAAAGCTGACTGACATATGCTATTTGACTAATTTGGACCTTTGCAATCCTCTTCTTGAACTTATATCATTTTTAATTATGGTTATTTACCTGATTTGTGGCTAAATATCAAATAACTAGTTGTGAAGCTTAGCTTGAACTTGGAGAAATAGAGGCTCATGAGTCGGAGCTTTTCCAGGTGGAAATGGTAAAATGCCGACTCAAAACAACTTGAACTATGACTTTTTTGAGTCCTGTATGTAAGCTACTTAATAAATACTGAGGTCTTGTGTGCCAAAAATTTTCAATAAGGTTGCACTTCCACTAACACTTTCAATGTTCCAAATAAGTTTTTCCCACTCGATTTCCAAAAAGTAAAAATTTATAAATAGTCAAGGGTGCTAAAACTAGATATTTTATCCATGCATATCAGGAGCATACTGTCATTGAATCAAAGCCTGATCATTTTCTGGACGATCTCCGGTTAAATAACCCATGGCCAGAGCTGAGGAGGTAGAGTCTTTAGTCAAAGCCTTTCTATATTTTTAATTGGATGGATTTTATGTGTGAATATTGTCGTTTGCATTTAGTTTCTGGTCTATCTAAGCCGTGAAATTTACTACCAAGTATGGCTACTGGTTTGAAGTTTCATTCTTTCAAGATTGTGATATTGCATGCACTCACCTCTTACAGGTTTGCTGAAAGTATTGATGTCAATGTTCCTGACCCTGTTGCTCACAAACACATTCCATATGTTGTTGTCCTTGTCAAAATGGCAGAAGAGTGGTCCAAAAGCCATGGCGGCAGTTTACCATCAAGTAGAGAGGAGAAGAAAGCGTTCAAGGTTGCATTACCTTTTTTTTAATTATTATTTTTAAATCAGAAAATACAAAAGCATACAAAAGGGCAAGGTTGCATTTCCTTTTTGCAATCAGTGATCTTTGACAGTTACTAAACTTATTGGCTCTTGGACAATGTAGGATCTTCTCAAAGCCAAGATGATTGCAATGGATGAAGATAACTACAGAGAAGCTATTGAAGCCTCTTTCAAAGTCTTTACTCCTCATGGAATCAGTAAGCAAGCATTTTTTTTTAAGCATATAGACTAATTGATTTAGAACACAAACCTGTTGTTGGTCTTTGATATTGGTTATGAAAAATGTTTTATCAGTTGCTTGTGTGTATTCTTGCCTTTCGGAACTTAGGCTAACTTCTATAGGCCTTTTGTTGAGAATCCATCTCTTTTTGACAAAGCAATGCCTAGAAGGTTCCTCGTCCCTGCAGACAAGTTTGATTACAGCTTATTTTTTAGGACCTTATAATGCTCTAGGTCCAGATGGGTGTTATTTGCTAAATTATTTAAGAGATGTTGAAATACTCTCAAGCATGATATTACGGAGGTGTTCCATGATTGCTTGAGAGTGGTATCATTATTGCTTGAGAGTAGGTTCTGGAATGGTCTCTGGCTTCAGTACATTAGCCTCACTACCTGTTTATAAATCGTCATTGCTAAAGTCCCGGTAGAAAGGCTCAGGAAGTTTCTTCCTTCAATTATCTCTATTCCCCAATTTGCTTCCATTGAAAATCGTTGAGTTATTGTTATTGCTAATTAGCTTATTGATGAATGGCAGAGAAATAGAAAGAAAGGTTTTCTTATTAAATTAGACCTAGAAAAATCCTTTGGTAAAGTGGACTAGTACTTCCTAGAAAAAGCCTCCAAGCTAAGGGCCTTAATCTTTGCTGGAGGAAGTGAATCTTCGTCTGCATCTTTAGTATCAATTGTTCCATTCTTTTGGATAGGAATTGTCCATGATCATTAATGGTAGGTGAGGGAAAATAACCCTTTTTGGTGGAATCGGACCATGGATTCTCTTTCTCCCTTTTCTTTAATTGTCTTGGTAGACTTCTTGATAAAGGTGCTTGATTTAGCTTGGTAGGACACTTTATTGTGGAGTTCCACTATTCTCAGGCTGAAATTTGGATAATCTTTCCAACATTTTCAATATTATAAAAAACTCTGGAGCTGCTTTAGGCCTTGATTCTGACTTCATTAAGATTGTTAATGCAGGCATCAATTATGAGGATTTCATTGTTAAACAAGTCGATTCTCTTTATGACTGAAGTGTCCCCTCCATCTTGTTCTTCCTTTAAATGGTAGACTGCAACCTACTCTTTTTGTCCTCCCATATTGGAGAAAAAGATTGAGTGCAAACTCTAAAATGGATCAATTTCCTCATTTCTGAAGTGGGCAACAGGGCAAGTTAGCTCCTATTTAAGCTAACTCTTGTAGATCTTCCTTACTGCATGCCTCTTTATCTCATGTTCGTTAAAGTTGCGAAGGGTATTGAGAGTATTGAAACTTTCTATGGGTAGGTATGTCTTCAGATGCTGGTTCTCACCTTGTCAAATGGTCCAAAGTTAGGTCTTGGTATAGGTAATATCTCGAAAAAGATGCTCCATGGAGGATTACTATGGGCAACATAATGGAATAGCAAGTGGTTGGGGAACATATCTATTTATACTTCTAAAGGGCCAGCATTCTATCTTCAAGCTCAAGAATATATCATCCTCCAAGAATTTTGTTGGAAATGGTGGTATGACTTGCTAATGGAATGATCACTGTATTTCTAGGGGAACTTTCTCTTTTTGCTCGTATGCCAGCGAAATCTGAGGAGAAAGCCTTCGTTTCCTCTCAATGGTTCAAACTAACGTCAACACTAGGTTCTCAGACTATCTTTTAGAAAGGAGAGGAAAATCTTGTGTATCACGAGACCTTTATTTGATACTTACAATTCGAAAGCAATAGTAGAATTTTCAAGATATTGAAGCATCCTTTCTTAAATTTTGGTATAATGTGATTTTTATTTCTTTTACTTGGTTCAAATTCCACCATCTGTTTTGTAATTATAGCTGAACAGTCTTTGTGTCAAAGACATTGGTAGTGGAACAAAAAATAAATCTGTAGGTCTTTCCTGGAACTGCTAGTTCAACTTCTCTTGCTCTGCTCAGAATTAGTTTTCATTTTACGTGGTCCAATCACTTCAGCATGTCATGGAAGGATTTTGGAAGACCACCACTTATTATTTATACCTAAAAGCTGAACTCTTAACTTTAGAAATTTTGAACATACGACATTGACTGTCTATGGTTTCAATATGTTTACAATTTTAGTGTTCAATGGTGTGTTTTAAGGTTTTTGTTGGAGTCTAATCTTTTTAGTCTTCCAAAGGTGACGGTTATAATCGGATGTCTAAAATATGAGCTTTATCATTTTCTTCCTATCAGGTCCAGATCTGAAACAGATAGTTGATGATAGCTGTGCTGAAGTTGATTCTAATTCATCGGATTTTTGGATATTAGTGGCTGCTTTGAAGGTTACTGAAAATTTTGCATTGTTACATTAGTTCTGTATCTTTTATGAAGTGTCTGTTTCTTTTCTCTCTCTCCCTCTCTTTTTTTTTTTTCTCCTAGAGTTCTTTAACTTGTTATTTGTGAAATTTTATATTCCAAATTCCAACACTTGACTGCTTCTCAGGCATTATTTCTAGTTGTGGAATTCAATCTTGTAAACTACTTGAGGTTCTTAGGATTGACCAGTCACCATCATCATACTTAACTTACCATTTTTCTAATGCATATGACTGCGAGATATGTAATTGATTTATTACATGGATATTGCTTGATTCTGTGCCATGCAGTTCATTGCTTAACAAGTAAGCCTTAGACCTTGGAAGGATCTGCTTGTGGATTTACTTATTTGTTCTATCCCATTTGGGGGATCAGGAAATTCGAGGGTAGATTCATTTGGATTAACATGCCATTAGATTACATTGGTAATTTAACTTTGCTTCTGGTTTAATCTTTTATTTTATAACAGTTAAAAATCTATTAAACATGTTAAACTTGTATTCCTTATGGTTTAAATCAATAATTTTATGAGTAATTCATGCATTACATATGATTTTAGTAAATTTCTAATATTCGTATAACATATAACTAAAAAGTCACAACTTATTTATTTTATTAATATTATCAACACTTCAATGTTTACTTTTTATTATTTTTTAAAACTTGTATGAAACAATGTATTGAAATCTATTGAACTGCTGCTACTGCTATTCTTCTCAAACTTGTTACCTGAACTTCATGCAGGAGTTCATAGTGAACGAAGGAGGTGGGGAGGCACCCATGGAGGGATCAATACCAGATATGACATCTTCAACTGAGTGAGTGATTCGATTTTCTTTGGAATACTATCAGTGGAATTTAATAAACTTATTTATCGACTGAAGGCTGGATTCCAATTTTTGTAGGCATTATGTTAATTTGCAAAACATCTACCAAGCCAAGGCCGAGGCTGACTTTATGGTAATAGATCAAAGAGCAAGGAACATTTTGAAAAAAATTGGCAGAGATCCAAATAGCATCTCAAAGACGACAACCAAAAGCTTCTGTAAAAATGCAAGAAAACTGAAAGTATGCACTGAACTATTATATTACTATTATTATTTTGTACAATCTGCTGGTATCTCAAGTTGATATGACATGGATTTTCTTATTATAAAGCTTCCATTCTCTCTTATCAAAAAAATATTATAATGCTTCCATTCTCCAAGATATGCTAATAATCTTCCTTTAAAGAGCCTAATTATGTTTTAGCCATCAAGAAATATGCGATCAGGGCATTTTTTACTTTGTCCTGGGTGTAGTTCTGGAGTCTATCACAGGACAGAAGTTTCTCCAACCGAATAGTTGTTGTCTCTATCTTGTTTTAAAAAAAATTGTTTCTCAATAAATTAAAAAAATTTTGAACTTTATTATACTTCAACTGGGTTGAAAGTATGGATGCATGCCTACAAATGCACGCATACATAGCTGTATTTTTTCTAATGCATTATGGGAAAATTTTGTAGGTTTGCAGATATCGTTCGCTTGAGGATGAGTTCAATTCTCCAGTCTTCACTGAGCTACAGAAGTATTTAACTGACGAGGATTTCAGGTGACACGAAATTTGTTAACGTGCTTCTTCTATTGCTTTCTGATGTTATGTTTTTACCATACTCAGTGGCAACGATGAATGAACTATATTATATAAGTTGTGGGCTACATTTGTATTCAAATACAAGGGAGTAAAAAACCGTATTCTTAGAAATTTATGGATATCATGATGTGGTTTTTGAAGGTTCTATTCTCAGAAGTGCTCACTTGGATGTGTCTAATGCATATAAGCATATGTTAACTCCTTGGCTACTATTCAAGCTTGACAGTTGATTAACTTTCTTAATCTCCTTTGATTGTTGTCTCTCTCTTAGTTTAGGATGGACGACAAGCTTTTGTTTACCGTTCATATTTTTCTTAGCAAAAACAAAACAAAATTTTTTATTCCCTTTATGTATTGGTGTAAAAACTATTTTTTATGCTGTTTATATCATTAATCTTAAATTTGGAGCATCATATTACATCCCTGCTTTCTAGAAGTATTTTCCATTTCAACATCTTCCTGCCGATTCTGGTCTATTTCTTTTCAGTTATAGAATTGTTTCATGCAACCAGTTACACCGGGAGTTGTCTGATTACTATTACATGGTCTATTTATTTATCCAAAACTTCTGCAATCAATTTATACGTTACCATGGAGTAGTCGGTTCCTCTGCATGTGCTTACCAGCATAGCTCTTGGAACTTCTCATCAATTGAATTCTATTTTATATATTTTTTCTTTTTTTTTAGCAGATTCTATTTTTTTTCTTTAAAGAAGTAATCTCTAATCTGCAAAATGTAATGAATTGAAAGGCACAAGTATCAGCCACACATTGTTAGTATGAATAGTTACAATATACTGATTTTGATTTCTCATTTTCCAGTGTCGCAGTTGGTTTCTATATTCTGCTTCGAGCTGTTGATCGTTTTGCTGCCAATTACAACAGTTTTCCTGGGCAATTTGATGGGTAATACCTCCTGCTGTTGTAAATGGATTATGTGTTCATCATTTGCCACAACTCACTCTGCATTGATGATGTATACTTTATAATTAATTAATGCCTTGCAGGGGGATGGATGAGGACATATCCCGATTGAAAACTACTGCCGTTGGCCTACTCAGTGACTTGGGTTGCAATGGCTTGACATTGTCAGAGGATCTCATTAATGAGATGTGCCGATTCGGCGCTGCGGAGCTCCATACAGTCGCTGCCTTCATTGGAGGGATTGCATCACAAGAAGTAATCAAGGTAACATTCACATACTTTCTGAGCCTTCTCTTATTTTTGATCTGTCAGTGCCAGTGGATATAGTAAGATTGGCAAATTAAATTTCATTCATCATTTCTAGTGTCGGTGGTCATTTGCTTATGGGGCTGCATTTGAAGTCAAATGAATTTGATATTACCCCACCAAAATTTACATTTTCATAAGAGTTTCTCTCCCCTAAAATGACGTATTAAAAAAGTTCTTTTTTCTTCAGCTAATCACCCGACAGTTTGTACCCATGTCTGGAACTTACGTATTCAATGGCATTGATCACAAGTCGCAGTTGTTGTCTCTCTAACGGGTTTTAAAGCCATAAAATGTTGAGAAAGAACTGGTATCCATCACCTTCTCCCCAATACCATCAACCTTGAAGTTTTGCTGGATTCCAGATTCACCTCTTCGGTTTTCATTGGCGGACCAGACACTTTCCCTGGAGGAGAAAGCGCTCACTTCTTATTTGGAGTTATGGTGCATTGTTGTATTCATTGCTCAATTCATACTTCGTGCAATTCCCTGTTGATGAAGGTTCGATTAGATCGAAATTTTGATCGTATCTCTGACTTTGCAGAGGGAGAGCAAAAGGGATTATTAGAAAGTTTCTGATTCTATATCTTATTTCTATCTTTATGACCTTCAGTCCTATGAGTATGACCTTTCAATGGCCTCCTCGAACCAATCTGGGTTTCAAAATATAACAATTATGATCGTCTTTGGGGCATTGCTGAATTTGGTGAACGTCATTTATCGTCTTTTCTTTATCATTGTTACTTTCTACCCAGTGAGTGTTCTGAGCTGTAACTATACCATTTCAGGAACTTGTTGCACTGGCACCTACCAACGCGTTGGGTATTAAATACACATATTCGGTTTAAGTATATGATTTTCAATTTTAAAATATAGTGATATTTAAATCCTATTGATCAACC

mRNA sequence

AGGATCTGGGGTGAACAAGGACAGGCAGCCCTTGAAAAAGCGAGTATATGCCTTCTCAACTGTGGCCCTACAGGTTCCGAGACTTTGAAAAATCTTGTTCTTGGAGGGATTGGAAGTATCACCATAGTTGACGGTACCAAGGTAGAAGAGGGCGATCTTGGCAACAACTTCATGGTGGATGAATCAAGTATCGGACAGTCAAAAGCAAAGTGTGTATGTGCATTTCTTCAAGAGCTAAATGATGCTGTAAAAGCTAAGTTTATAGAAGAGTTTCCAGAGGCGTTAATTGAAACAAGACCATCATTCTTTTCTCAGTTTACGTTAGTAGTTGCTACACAGTTGGGTGAGGAGTGGATTGTAAAGCTTGACAGAATCTGTAGGAAGGCAAATGTCATATTGATTGTTGCACGCTCCTACGGCCTTACTGGGCTTGTCAGAATCAGTTTAAAGGAGCATACTGTCATTGAATCAAAGCCTGATCATTTTCTGGACGATCTCCGGTTAAATAACCCATGGCCAGAGCTGAGGAGGTTTGCTGAAAGTATTGATGTCAATGTTCCTGACCCTGTTGCTCACAAACACATTCCATATGTTGTTGTCCTTGTCAAAATGGCAGAAGAGTGGTCCAAAAGCCATGGCGGCAGTTTACCATCAAGTAGAGAGGAGAAGAAAGCGTTCAAGGATCTTCTCAAAGCCAAGATGATTGCAATGGATGAAGATAACTACAGAGAAGCTATTGAAGCCTCTTTCAAAGTCTTTACTCCTCATGGAATCAGTCCAGATCTGAAACAGATAGTTGATGATAGCTGTGCTGAAGTTGATTCTAATTCATCGGATTTTTGGATATTAGTGGCTGCTTTGAAGGAGTTCATAGTGAACGAAGGAGGTGGGGAGGCACCCATGGAGGGATCAATACCAGATATGACATCTTCAACTGAGCATTATGTTAATTTGCAAAACATCTACCAAGCCAAGGCCGAGGCTGACTTTATGGTAATAGATCAAAGAGCAAGGAACATTTTGAAAAAAATTGGCAGAGATCCAAATAGCATCTCAAAGACGACAACCAAAAGCTTCTGTAAAAATGCAAGAAAACTGAAAGTTTGCAGATATCGTTCGCTTGAGGATGAGTTCAATTCTCCAGTCTTCACTGAGCTACAGAAGTATTTAACTGACGAGGATTTCAGTGTCGCAGTTGGTTTCTATATTCTGCTTCGAGCTGTTGATCGTTTTGCTGCCAATTACAACAGTTTTCCTGGGCAATTTGATGGGGGGATGGATGAGGACATATCCCGATTGAAAACTACTGCCGTTGGCCTACTCAGTGACTTGGGTTGCAATGGCTTGACATTGTCAGAGGATCTCATTAATGAGATGTGCCGATTCGGCGCTGCGGAGCTCCATACAGTCGCTGCCTTCATTGGAGGGATTGCATCACAAGAAGTAATCAAGCTAATCACCCGACAGTTTGTACCCATGTCTGGAACTTACGTATTCAATGGCATTGATCACAAGTCGCAGTTGTTGTCTCTCTAACGGGTTTTAAAGCCATAAAATGTTGAGAAAGAACTGGTATCCATCACCTTCTCCCCAATACCATCAACCTTGAAGTTTTGCTGGATTCCAGATTCACCTCTTCGGTTTTCATTGGCGGACCAGACACTTTCCCTGGAGGAGAAAGCGCTCACTTCTTATTTGGAGTTATGGTGCATTGTTGTATTCATTGCTCAATTCATACTTCGTGCAATTCCCTGTTGATGAAGGTTCGATTAGATCGAAATTTTGATCGTATCTCTGACTTTGCAGAGGGAGAGCAAAAGGGATTATTAGAAAGTTTCTGATTCTATATCTTATTTCTATCTTTATGACCTTCAGTCCTATGAGTATGACCTTTCAATGGCCTCCTCGAACCAATCTGGGTTTCAAAATATAACAATTATGATCGTCTTTGGGGCATTGCTGAATTTGGTGAACGTCATTTATCGTCTTTTCTTTATCATTGTTACTTTCTACCCAGTGAGTGTTCTGAGCTGTAACTATACCATTTCAGGAACTTGTTGCACTGGCACCTACCAACGCGTTGGGTATTAAATACACATATTCGGTTTAAGTATATGATTTTCAATTTTAAAATATAGTGATATTTAAATCCTATTGATCAACC

Coding sequence (CDS)

AGGATCTGGGGTGAACAAGGACAGGCAGCCCTTGAAAAAGCGAGTATATGCCTTCTCAACTGTGGCCCTACAGGTTCCGAGACTTTGAAAAATCTTGTTCTTGGAGGGATTGGAAGTATCACCATAGTTGACGGTACCAAGGTAGAAGAGGGCGATCTTGGCAACAACTTCATGGTGGATGAATCAAGTATCGGACAGTCAAAAGCAAAGTGTGTATGTGCATTTCTTCAAGAGCTAAATGATGCTGTAAAAGCTAAGTTTATAGAAGAGTTTCCAGAGGCGTTAATTGAAACAAGACCATCATTCTTTTCTCAGTTTACGTTAGTAGTTGCTACACAGTTGGGTGAGGAGTGGATTGTAAAGCTTGACAGAATCTGTAGGAAGGCAAATGTCATATTGATTGTTGCACGCTCCTACGGCCTTACTGGGCTTGTCAGAATCAGTTTAAAGGAGCATACTGTCATTGAATCAAAGCCTGATCATTTTCTGGACGATCTCCGGTTAAATAACCCATGGCCAGAGCTGAGGAGGTTTGCTGAAAGTATTGATGTCAATGTTCCTGACCCTGTTGCTCACAAACACATTCCATATGTTGTTGTCCTTGTCAAAATGGCAGAAGAGTGGTCCAAAAGCCATGGCGGCAGTTTACCATCAAGTAGAGAGGAGAAGAAAGCGTTCAAGGATCTTCTCAAAGCCAAGATGATTGCAATGGATGAAGATAACTACAGAGAAGCTATTGAAGCCTCTTTCAAAGTCTTTACTCCTCATGGAATCAGTCCAGATCTGAAACAGATAGTTGATGATAGCTGTGCTGAAGTTGATTCTAATTCATCGGATTTTTGGATATTAGTGGCTGCTTTGAAGGAGTTCATAGTGAACGAAGGAGGTGGGGAGGCACCCATGGAGGGATCAATACCAGATATGACATCTTCAACTGAGCATTATGTTAATTTGCAAAACATCTACCAAGCCAAGGCCGAGGCTGACTTTATGGTAATAGATCAAAGAGCAAGGAACATTTTGAAAAAAATTGGCAGAGATCCAAATAGCATCTCAAAGACGACAACCAAAAGCTTCTGTAAAAATGCAAGAAAACTGAAAGTTTGCAGATATCGTTCGCTTGAGGATGAGTTCAATTCTCCAGTCTTCACTGAGCTACAGAAGTATTTAACTGACGAGGATTTCAGTGTCGCAGTTGGTTTCTATATTCTGCTTCGAGCTGTTGATCGTTTTGCTGCCAATTACAACAGTTTTCCTGGGCAATTTGATGGGGGGATGGATGAGGACATATCCCGATTGAAAACTACTGCCGTTGGCCTACTCAGTGACTTGGGTTGCAATGGCTTGACATTGTCAGAGGATCTCATTAATGAGATGTGCCGATTCGGCGCTGCGGAGCTCCATACAGTCGCTGCCTTCATTGGAGGGATTGCATCACAAGAAGTAATCAAGCTAATCACCCGACAGTTTGTACCCATGTCTGGAACTTACGTATTCAATGGCATTGATCACAAGTCGCAGTTGTTGTCTCTCTAA

Protein sequence

RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVDESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIVKLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAESIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDEDNYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFCKNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPGQFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQEVIKLITRQFVPMSGTYVFNGIDHKSQLLSL
Homology
BLAST of MC02g0458 vs. ExPASy Swiss-Prot
Match: P42744 (NEDD8-activating enzyme E1 regulatory subunit AXR1 OS=Arabidopsis thaliana OX=3702 GN=AXR1 PE=1 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 7.5e-238
Identity = 392/512 (76.56%), Postives = 460/512 (89.84%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGE GQAALE+ASICLLNCGPTGSE LKNLVLGG+GSIT+VDG+KV+ GDLGNNFMVD
Sbjct: 29  RIWGEVGQAALEEASICLLNCGPTGSEALKNLVLGGVGSITVVDGSKVQFGDLGNNFMVD 88

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
             S+GQSKAK VCAFLQELND+V AKFIEE P+ LI T PSFFSQFTLV+ATQL E+ ++
Sbjct: 89  AKSVGQSKAKSVCAFLQELNDSVNAKFIEENPDTLITTNPSFFSQFTLVIATQLVEDSML 148

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLDRICR ANV L++ RSYGL G VRIS+KEH +I+SKPDHFLDDLRLNNPWPEL+ F E
Sbjct: 149 KLDRICRDANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRLNNPWPELKSFVE 208

Query: 181 SIDVNVPDP-VAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDE 240
           +ID+NV +P  AHKHIPYVV+LVKMAEEW++SH G+LPS+REEKK FKDL+K+KM++ DE
Sbjct: 209 TIDLNVSEPAAAHKHIPYVVILVKMAEEWAQSHSGNLPSTREEKKEFKDLVKSKMVSTDE 268

Query: 241 DNYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEA 300
           DNY+EAIEA+FKVF P GIS +++++++DSCAEV+SNSS FW++VAALKEF++NEGGGEA
Sbjct: 269 DNYKEAIEAAFKVFAPRGISSEVQKLINDSCAEVNSNSSAFWVMVAALKEFVLNEGGGEA 328

Query: 301 PMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSF 360
           P+EGSIPDMTSSTEHY+NLQ IY AKAEADF+VI++R +NILKKIGRDP+SI K T KSF
Sbjct: 329 PLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVIEERVKNILKKIGRDPSSIPKPTIKSF 388

Query: 361 CKNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFP 420
           CKNARKLK+CRYR +EDEF +P  TE+QKYL DED+S A+GFYILLRA DRFAANYN FP
Sbjct: 389 CKNARKLKLCRYRMVEDEFRNPSVTEIQKYLADEDYSGAMGFYILLRAADRFAANYNKFP 448

Query: 421 GQFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIAS 480
           GQFDGGMDEDISRLKTTA+ LL+DLGCNG  L +DLI+EMCRFGA+E+H V+AF+GGIAS
Sbjct: 449 GQFDGGMDEDISRLKTTALSLLTDLGCNGSVLPDDLIHEMCRFGASEIHVVSAFVGGIAS 508

Query: 481 QEVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 512
           QEVIKL+T+QFVPM GTY+FNGIDHKSQLL L
Sbjct: 509 QEVIKLVTKQFVPMLGTYIFNGIDHKSQLLKL 540

BLAST of MC02g0458 vs. ExPASy Swiss-Prot
Match: Q9ZV69 (NEDD8-activating enzyme E1 regulatory subunit AXL OS=Arabidopsis thaliana OX=3702 GN=AXL1 PE=1 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 2.1e-224
Identity = 372/511 (72.80%), Postives = 441/511 (86.30%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGE GQ+ALE ASICLLNCGPTGSE LKNLV+GGIGSITIVDG+KVE GDLGNNFMVD
Sbjct: 13  RIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKVEIGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
             S+GQS+AK VC FLQELND+VKA F+EE P+ LI T PSFFSQFTLV+ATQL E+ +V
Sbjct: 73  AKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTLVIATQLVEDSMV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLDRICR+ANV+L++ARSYGLTG VRIS+KEHT IE+KPDH LDDLRLN+PWPEL+ + E
Sbjct: 133 KLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRLNSPWPELKSYVE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           SID+NV +P AHKHIPYVV+LVK+AEEW++ H G+LPS+REEK  FKDL+K+KM++ DE+
Sbjct: 193 SIDLNVEEPAAHKHIPYVVILVKVAEEWAQHHSGNLPSTREEKNEFKDLVKSKMVSADEE 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NY+EA+ A+FKVF P GIS +++ I  DSCAEV SNSSDFW++VAALKEFI NEGGGE P
Sbjct: 253 NYKEALLAAFKVFAPTGISQEIQDINHDSCAEVGSNSSDFWVMVAALKEFISNEGGGEVP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           +EGS+PDM SSTEHY+NLQ IY +KAEADF+ ++QR ++IL K+G+DP+SISK T KSFC
Sbjct: 313 LEGSMPDMISSTEHYINLQKIYHSKAEADFLSMEQRVKSILVKVGQDPSSISKPTIKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYR++EDEF SP  TEL KYL DE++S A+GFYILLRAVDRFA  Y  FPG
Sbjct: 373 KNARKLKVCRYRTIEDEFKSPSTTELHKYLADENYSGAIGFYILLRAVDRFAGTYKKFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDG  DED S+LKT A+ LLS++GC+G  L E+L NEMCRFGAAE+H VAA IGGI SQ
Sbjct: 433 QFDGSTDEDASQLKTIALSLLSEMGCDGYELQEELYNEMCRFGAAEIHVVAALIGGITSQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 512
           EVIKLIT+QFVP  GT++FNGIDHKSQ L+L
Sbjct: 493 EVIKLITKQFVPKRGTFIFNGIDHKSQSLTL 523

BLAST of MC02g0458 vs. ExPASy Swiss-Prot
Match: Q54JM3 (NEDD8-activating enzyme E1 regulatory subunit OS=Dictyostelium discoideum OX=44689 GN=nae1 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 3.5e-118
Identity = 216/503 (42.94%), Postives = 310/503 (61.63%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           R+WGE GQ+ LE++ I LLN   TG+ETLKNLVL GIGS T+VD  KV E DLGNNF V+
Sbjct: 16  RLWGEDGQSKLERSHILLLNGSATGTETLKNLVLPGIGSFTVVDNKKVTESDLGNNFFVE 75

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
            SS+G+ +A  VC  L+ELND VK   +EE P  LI    SFF  F+LVVA +L EE ++
Sbjct: 76  RSSLGKPRATVVCELLRELNDRVKGFSVEECPIHLINNNISFFKDFSLVVANRLSEEALL 135

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
            L +   + N+ L++  SYG  G +RIS  EH +IESKPD  +DDLR+ NP+ +L   A+
Sbjct: 136 TLSQYLTEQNIPLLITNSYGYIGYLRISTPEHQIIESKPDDPIDDLRIYNPFKQLVDMAD 195

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +++++  +   H H+PYV++L+K  +EW  +H   +P +R EK  FK    +   + DE 
Sbjct: 196 ALELDKLNTQQHSHVPYVLLLIKFLKEWRSTHNDKMPETRAEKDEFKKFFNSHSWSADEM 255

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           N+ E I+   K   P  +  D++ ++ D    +  NS DFW+LVAALKEF+ N      P
Sbjct: 256 NFVEGIQNLLKYIQPPRVPGDVQNLLKDPKTNITENSDDFWVLVAALKEFMTN-NDNTLP 315

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           + G++PDMTS T +++ LQ  YQ KA AD          IL K+G+  +SIS    K FC
Sbjct: 316 LHGNVPDMTSETHNFIQLQKGYQEKALADLSEFSGYVDQILTKVGK--SSISSDLVKKFC 375

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KN R L + RYR++ +E+N P    ++  L   D    + FYILLR +D+F   Y+ +PG
Sbjct: 376 KNTRFLNIIRYRTISEEYNQPKTNLIKSELEQAD--TVMVFYILLRGIDKFYKTYHKYPG 435

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
             D   + DI  LKT     L+++  +   + +D I E  RFG +ELH +A+ +GG+ SQ
Sbjct: 436 SSD-DFESDIPLLKTVITQYLAEINISNDLVKDDYIAEFVRFGGSELHNIASLMGGVTSQ 495

Query: 481 EVIKLITRQFVPMSGTYVFNGID 504
           E+IKLIT Q+ P++ T++FNGI+
Sbjct: 496 EIIKLITHQYTPLNNTFIFNGIN 512

BLAST of MC02g0458 vs. ExPASy Swiss-Prot
Match: Q6NTW6 (NEDD8-activating enzyme E1 regulatory subunit OS=Xenopus laevis OX=8355 GN=nae1 PE=2 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.3e-117
Identity = 213/518 (41.12%), Postives = 326/518 (62.93%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           R+WG+ GQ ALE A +CL+N   TG+E LKNLVL GIG  TIVDG  V   D+GNNF + 
Sbjct: 17  RLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGLFTIVDGNVVAGEDVGNNFFLQ 76

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           + SIG+++A+     LQELND V   F+ E PE L++  PSFF +FT+V++TQL E  ++
Sbjct: 77  KESIGKNRAQTSMELLQELNDDVTGNFVPESPEQLLDKDPSFFCKFTMVISTQLPESTLL 136

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           +L      +N+ L+V R+YG  G +RI +KEHTVIES PD+ L+DLRL+ P+PELR   +
Sbjct: 137 RLAETLWDSNIPLLVCRAYGFVGYMRIIVKEHTVIESHPDNALEDLRLDQPFPELREHLQ 196

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKM------ 240
           S D++  +   H H P+++V+ K  ++W   +GG +P S +EK++F+DL++  +      
Sbjct: 197 SYDLDHMERKDHSHTPWIIVVAKYLDKWRSENGGQMPKSYKEKESFRDLIRQGILKNENG 256

Query: 241 IAMDEDNYREAIEASFKVFTPHGISPDLKQIV-DDSCAEVDSNSSDFWILVAALKEFIVN 300
           +  DE+N+ EAI+          +S  +++I+ DD C  +   S+ FWIL  A+KEF+  
Sbjct: 257 VPEDEENFEEAIKNVNTALNITKVSSSVEEILNDDRCTNLTHQSTSFWILARAVKEFMAM 316

Query: 301 EGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISK 360
           EG G  P+ G+IPDM + ++ ++ LQNIY+ KA+ D   ++     +L+ +GR P SIS+
Sbjct: 317 EGKGNLPLRGTIPDMIADSDKFIKLQNIYREKAKKDASAVESCVSKLLQSVGRPPESISE 376

Query: 361 TTTKSFCKNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAA 420
              + FC+N   L+V R RSLE+E+      +       E+    +  Y++LRAVDRF  
Sbjct: 377 RDIRLFCRNCAFLRVVRCRSLEEEYGLDTAKKDDIVSLMENPDNEIVLYLMLRAVDRFQK 436

Query: 421 NYNSFPGQFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAF 480
               +PG ++  ++ DI +LK+   GLL + G + LT+ ++ I E CR+GAAE HT+A+F
Sbjct: 437 QLGRYPGIYNYQIESDIGKLKSCLNGLLQEYGLS-LTVKDEYIQEFCRYGAAEPHTIASF 496

Query: 481 IGGIASQEVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 512
           +GG A+QE IK+IT+QFV  + T+++N +   S    L
Sbjct: 497 LGGAAAQEAIKIITKQFVIFNNTFIYNAMLQTSATFQL 533

BLAST of MC02g0458 vs. ExPASy Swiss-Prot
Match: Q8VBW6 (NEDD8-activating enzyme E1 regulatory subunit OS=Mus musculus OX=10090 GN=Nae1 PE=1 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.7e-117
Identity = 212/518 (40.93%), Postives = 320/518 (61.78%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           R+WG+ GQ ALE A +CL+N   TG+E LKNLVL GIGS TI+DG  V   D GNNF + 
Sbjct: 18  RLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNLVSGEDAGNNFFLQ 77

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           +SSIG+++A+    FLQELN  V   F+EE PE L++  PSFF +FT+VVATQL E  ++
Sbjct: 78  KSSIGKNRAQAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLLESTLL 137

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           +L  +   + + L++ R+YGL G +RI +KEH VIES PD+ L+DLRL+ P+PELR   +
Sbjct: 138 RLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHLQ 197

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMI----- 240
           S D++  +   H H P++V++ K   +W     G +P S +EK+ F+DL++  ++     
Sbjct: 198 SYDLDHMEKKDHSHTPWIVIIAKYLAQWYNETNGRIPKSYKEKEDFRDLIRQGILKNENG 257

Query: 241 -AMDEDNYREAIEASFKVFTPHGISPDLKQIV-DDSCAEVDSNSSDFWILVAALKEFIVN 300
              DE+N+ EAI+          I   ++ I  DD C  +   +  FWIL  ALKEF+  
Sbjct: 258 APEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAK 317

Query: 301 EGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISK 360
           EG G  P+ G+IPDM + +  Y+ LQN+Y+ KA+ D   +      +L+ +G+ P SIS+
Sbjct: 318 EGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAVGNHVAKLLQSVGQAPESISE 377

Query: 361 TTTKSFCKNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAA 420
              K  C N+  L+V R RSL +E+      + +   + ++    +  Y++LRAVDRF  
Sbjct: 378 KELKLLCSNSAFLRVVRCRSLAEEYGLDTVNKDEIISSMDNPDNEIVLYLMLRAVDRFHK 437

Query: 421 NYNSFPGQFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAF 480
            +  +PG  +  ++EDI +LK+   G L + G + + + +D ++E CR+GAAE HT+AAF
Sbjct: 438 QHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLS-VMVKDDYVHEFCRYGAAEPHTIAAF 497

Query: 481 IGGIASQEVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 512
           +GG A+QEVIK+IT+QFV  + TY+++G+   S    L
Sbjct: 498 LGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL 534

BLAST of MC02g0458 vs. NCBI nr
Match: XP_022140497.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1 [Momordica charantia])

HSP 1 Score: 1013 bits (2620), Expect = 0.0
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD
Sbjct: 13  RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE
Sbjct: 133 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED
Sbjct: 193 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP
Sbjct: 253 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ
Sbjct: 433 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. NCBI nr
Match: XP_023512938.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 487/511 (95.30%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTK+EEGDLGNNFMVD
Sbjct: 13  RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTLVVATQL EEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQLVEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLD+ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRF+E
Sbjct: 133 KLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFSE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +IDVN+PDPVAHKH PYVV+LVKMAEEW+KSHGGSLPSSREEKK FKDLLKAKMIAMDED
Sbjct: 193 TIDVNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NYREAIEASFKVF PHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFI NEGGGEAP
Sbjct: 253 NYREAIEASFKVFAPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIANEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADFM+I++RARNILKKIGRDPNSISKTT KSFC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDPNSISKTTIKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYRSLEDEFNSP+F ELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGG+DEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH VAAFIGGIASQ
Sbjct: 433 QFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHAVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGT+VFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. NCBI nr
Match: XP_022944430.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita moschata] >KAG7010733.1 NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 485/511 (94.91%), Postives = 500/511 (97.85%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTK+EEGDLGNNFMVD
Sbjct: 13  RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTLVVATQL EEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQLVEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLD+ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRF+E
Sbjct: 133 KLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFSE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +ID+N+PDPVAHKH PYVV+LVKMAEEW+KSHGGSLPSSREEKK FKDLLKAKMIAMDED
Sbjct: 193 TIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NYREAIEASFKVF PHGISPDLKQIVDD CAEVDSNSSDFWILVAALKEFI NEGGGEAP
Sbjct: 253 NYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALKEFIANEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADFM+I++RARNILKKIGRDPNSISKTT KSFC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDPNSISKTTIKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYRSLEDEFNSP+F ELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGG+DEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH VAAFIGGIASQ
Sbjct: 433 QFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHAVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGT+VFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. NCBI nr
Match: XP_022986290.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita maxima])

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 484/511 (94.72%), Postives = 500/511 (97.85%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSIT+VDGTK+EEGDLGNNFMVD
Sbjct: 13  RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITLVDGTKIEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTLVVATQL EEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQLVEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLD+ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRF+E
Sbjct: 133 KLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFSE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +ID+N+PDPVAHKH PYVV+LVKMAEEW+KSHGGSLPSSREEKK FKDLLKAKMIAMDED
Sbjct: 193 TIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NYREAIEASFKVF PHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFI NEGGGEAP
Sbjct: 253 NYREAIEASFKVFAPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIANEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADFM+I++RARNILKKIGRDPNSISKTT K FC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDPNSISKTTIKGFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYRSLEDEFNSP+F ELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGG+DEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH VAAFIGGIASQ
Sbjct: 433 QFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHAVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGT+VFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. NCBI nr
Match: KAG6570885.1 (NEDD8-activating enzyme E1 regulatory subunit AXR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 479/505 (94.85%), Postives = 494/505 (97.82%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTK+EEGDLGNNFMVD
Sbjct: 13  RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTLVVATQL EEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQLVEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLD+ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRF+E
Sbjct: 133 KLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFSE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +ID+N+PDPVAHKH PYVV+LVKMAEEW+KSHGGSLPSSREEKK FKDLLKAKMIAMDED
Sbjct: 193 TIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NYREAIEASFKVF PHGISPDLKQIVDD CAEVDSNSSDFWILVAALKEFI NEGGGEAP
Sbjct: 253 NYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALKEFIANEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADFM+I++RARNILKKIGRDPNSISKTT KSFC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDPNSISKTTIKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYRSLEDEFNSP+F ELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGG+DEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH VAAFIGGIASQ
Sbjct: 433 QFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHAVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHK 505
           EVIKLITRQFVPMSGT+VFNGIDHK
Sbjct: 493 EVIKLITRQFVPMSGTFVFNGIDHK 517

BLAST of MC02g0458 vs. ExPASy TrEMBL
Match: A0A6J1CH54 (NEDD8-activating enzyme E1 regulatory subunit OS=Momordica charantia OX=3673 GN=LOC111011149 PE=3 SV=1)

HSP 1 Score: 1013 bits (2620), Expect = 0.0
Identity = 511/511 (100.00%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD
Sbjct: 13  RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE
Sbjct: 133 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED
Sbjct: 193 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP
Sbjct: 253 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ
Sbjct: 433 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. ExPASy TrEMBL
Match: A0A6J1FYN4 (NEDD8-activating enzyme E1 regulatory subunit OS=Cucurbita moschata OX=3662 GN=LOC111448883 PE=3 SV=1)

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 485/511 (94.91%), Postives = 500/511 (97.85%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTK+EEGDLGNNFMVD
Sbjct: 13  RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTLVVATQL EEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQLVEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLD+ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRF+E
Sbjct: 133 KLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFSE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +ID+N+PDPVAHKH PYVV+LVKMAEEW+KSHGGSLPSSREEKK FKDLLKAKMIAMDED
Sbjct: 193 TIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NYREAIEASFKVF PHGISPDLKQIVDD CAEVDSNSSDFWILVAALKEFI NEGGGEAP
Sbjct: 253 NYREAIEASFKVFAPHGISPDLKQIVDDCCAEVDSNSSDFWILVAALKEFIANEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADFM+I++RARNILKKIGRDPNSISKTT KSFC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDPNSISKTTIKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYRSLEDEFNSP+F ELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGG+DEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH VAAFIGGIASQ
Sbjct: 433 QFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHAVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGT+VFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. ExPASy TrEMBL
Match: A0A6J1JDN6 (NEDD8-activating enzyme E1 regulatory subunit OS=Cucurbita maxima OX=3661 GN=LOC111484081 PE=3 SV=1)

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 484/511 (94.72%), Postives = 500/511 (97.85%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSIT+VDGTK+EEGDLGNNFMVD
Sbjct: 13  RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITLVDGTKIEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEE PEALIETRPSFFSQFTLVVATQL EEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEECPEALIETRPSFFSQFTLVVATQLVEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLD+ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRF+E
Sbjct: 133 KLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFSE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +ID+N+PDPVAHKH PYVV+LVKMAEEW+KSHGGSLPSSREEKK FKDLLKAKMIAMDED
Sbjct: 193 TIDLNIPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKEFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NYREAIEASFKVF PHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFI NEGGGEAP
Sbjct: 253 NYREAIEASFKVFAPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIANEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADFM+I++RARNILKKIGRDPNSISKTT K FC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMLIEERARNILKKIGRDPNSISKTTIKGFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYRSLEDEFNSP+F ELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLKVCRYRSLEDEFNSPIFAELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGG+DEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH VAAFIGGIASQ
Sbjct: 433 QFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHAVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGT+VFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. ExPASy TrEMBL
Match: A0A0A0LMV7 (NEDD8-activating enzyme E1 regulatory subunit OS=Cucumis sativus OX=3659 GN=Csa_2G418860 PE=3 SV=1)

HSP 1 Score: 964 bits (2491), Expect = 0.0
Identity = 479/511 (93.74%), Postives = 498/511 (97.46%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWG+QGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTK+EEGDLGNNFMVD
Sbjct: 13  RIWGDQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEE+PEALIETRPSFFSQFTLVVATQL EEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEYPEALIETRPSFFSQFTLVVATQLVEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLD+ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE
Sbjct: 133 KLDKICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +ID+N PDPVAHKH PYVV+LVKMAEEW+KSHGGSLPSSREEKKAFKDLLKAKMIAMDED
Sbjct: 193 TIDLNAPDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NY+EAIEASFKVF P GIS DLKQIV+DSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP
Sbjct: 253 NYKEAIEASFKVFAPRGISSDLKQIVNDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADF +I++RARNILKKIGRDPNSISKTT KSFC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFKIIEERARNILKKIGRDPNSISKTTVKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKL+VCRYRSLEDEFNSP+  ELQKYLTDEDFSVAVGFY+LLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLRVCRYRSLEDEFNSPIVPELQKYLTDEDFSVAVGFYLLLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGG+DEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELH VAAF GGIASQ
Sbjct: 433 QFDGGIDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHVVAAFTGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGT+VFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. ExPASy TrEMBL
Match: A0A1S3C1I6 (NEDD8-activating enzyme E1 regulatory subunit OS=Cucumis melo OX=3656 GN=LOC103495961 PE=3 SV=1)

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 475/511 (92.95%), Postives = 492/511 (96.28%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWG+QGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTK+EEGDLGNNFMVD
Sbjct: 13  RIWGDQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKIEEGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
           ESSIGQSKAKCVCAFLQELNDAVKAKFIEE+PEALIETRP FFSQFTLVVATQL EEWIV
Sbjct: 73  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEYPEALIETRPWFFSQFTLVVATQLVEEWIV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLD ICR ANVIL+VARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE
Sbjct: 133 KLDNICRNANVILVVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           +ID+N  DPVAHKH PYVV+LVKMAEEW+KSHGGSLPS REEKKAFKDLLKAKMIAMDED
Sbjct: 193 TIDLNASDPVAHKHTPYVVILVKMAEEWAKSHGGSLPSGREEKKAFKDLLKAKMIAMDED 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NY+EAIEASFKVF P GIS DLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP
Sbjct: 253 NYKEAIEASFKVFAPRGISSDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           MEGSIPDMTSSTEHYVNLQNIYQAKAEADF +I++RARNILKKIGRDPNSISKTT KSFC
Sbjct: 313 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFKIIEERARNILKKIGRDPNSISKTTIKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKL+VCRYRSL DEFNSP+  ELQK LTDEDFSVAVGFY+LLRAVDRFAANYNSFPG
Sbjct: 373 KNARKLRVCRYRSLADEFNSPIVPELQKNLTDEDFSVAVGFYLLLRAVDRFAANYNSFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDGG+DEDISRLKTTAV LLSDLGCNGLTLSEDLINEMCRFGAAELH VAAFIGGIASQ
Sbjct: 433 QFDGGIDEDISRLKTTAVSLLSDLGCNGLTLSEDLINEMCRFGAAELHVVAAFIGGIASQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 511
           EVIKLITRQFVPMSGT+VFNGIDHKSQLLSL
Sbjct: 493 EVIKLITRQFVPMSGTFVFNGIDHKSQLLSL 523

BLAST of MC02g0458 vs. TAIR 10
Match: AT1G05180.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 824.3 bits (2128), Expect = 5.3e-239
Identity = 392/512 (76.56%), Postives = 460/512 (89.84%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGE GQAALE+ASICLLNCGPTGSE LKNLVLGG+GSIT+VDG+KV+ GDLGNNFMVD
Sbjct: 29  RIWGEVGQAALEEASICLLNCGPTGSEALKNLVLGGVGSITVVDGSKVQFGDLGNNFMVD 88

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
             S+GQSKAK VCAFLQELND+V AKFIEE P+ LI T PSFFSQFTLV+ATQL E+ ++
Sbjct: 89  AKSVGQSKAKSVCAFLQELNDSVNAKFIEENPDTLITTNPSFFSQFTLVIATQLVEDSML 148

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLDRICR ANV L++ RSYGL G VRIS+KEH +I+SKPDHFLDDLRLNNPWPEL+ F E
Sbjct: 149 KLDRICRDANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRLNNPWPELKSFVE 208

Query: 181 SIDVNVPDP-VAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDE 240
           +ID+NV +P  AHKHIPYVV+LVKMAEEW++SH G+LPS+REEKK FKDL+K+KM++ DE
Sbjct: 209 TIDLNVSEPAAAHKHIPYVVILVKMAEEWAQSHSGNLPSTREEKKEFKDLVKSKMVSTDE 268

Query: 241 DNYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEA 300
           DNY+EAIEA+FKVF P GIS +++++++DSCAEV+SNSS FW++VAALKEF++NEGGGEA
Sbjct: 269 DNYKEAIEAAFKVFAPRGISSEVQKLINDSCAEVNSNSSAFWVMVAALKEFVLNEGGGEA 328

Query: 301 PMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSF 360
           P+EGSIPDMTSSTEHY+NLQ IY AKAEADF+VI++R +NILKKIGRDP+SI K T KSF
Sbjct: 329 PLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVIEERVKNILKKIGRDPSSIPKPTIKSF 388

Query: 361 CKNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFP 420
           CKNARKLK+CRYR +EDEF +P  TE+QKYL DED+S A+GFYILLRA DRFAANYN FP
Sbjct: 389 CKNARKLKLCRYRMVEDEFRNPSVTEIQKYLADEDYSGAMGFYILLRAADRFAANYNKFP 448

Query: 421 GQFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIAS 480
           GQFDGGMDEDISRLKTTA+ LL+DLGCNG  L +DLI+EMCRFGA+E+H V+AF+GGIAS
Sbjct: 449 GQFDGGMDEDISRLKTTALSLLTDLGCNGSVLPDDLIHEMCRFGASEIHVVSAFVGGIAS 508

Query: 481 QEVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 512
           QEVIKL+T+QFVPM GTY+FNGIDHKSQLL L
Sbjct: 509 QEVIKLVTKQFVPMLGTYIFNGIDHKSQLLKL 540

BLAST of MC02g0458 vs. TAIR 10
Match: AT2G32410.1 (AXR1-like )

HSP 1 Score: 779.6 bits (2012), Expect = 1.5e-225
Identity = 372/511 (72.80%), Postives = 441/511 (86.30%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGE GQ+ALE ASICLLNCGPTGSE LKNLV+GGIGSITIVDG+KVE GDLGNNFMVD
Sbjct: 13  RIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKVEIGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
             S+GQS+AK VC FLQELND+VKA F+EE P+ LI T PSFFSQFTLV+ATQL E+ +V
Sbjct: 73  AKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTLVIATQLVEDSMV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLDRICR+ANV+L++ARSYGLTG VRIS+KEHT IE+KPDH LDDLRLN+PWPEL+ + E
Sbjct: 133 KLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRLNSPWPELKSYVE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           SID+NV +P AHKHIPYVV+LVK+AEEW++ H G+LPS+REEK  FKDL+K+KM++ DE+
Sbjct: 193 SIDLNVEEPAAHKHIPYVVILVKVAEEWAQHHSGNLPSTREEKNEFKDLVKSKMVSADEE 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NY+EA+ A+FKVF P GIS +++ I  DSCAEV SNSSDFW++VAALKEFI NEGGGE P
Sbjct: 253 NYKEALLAAFKVFAPTGISQEIQDINHDSCAEVGSNSSDFWVMVAALKEFISNEGGGEVP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           +EGS+PDM SSTEHY+NLQ IY +KAEADF+ ++QR ++IL K+G+DP+SISK T KSFC
Sbjct: 313 LEGSMPDMISSTEHYINLQKIYHSKAEADFLSMEQRVKSILVKVGQDPSSISKPTIKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAVGFYILLRAVDRFAANYNSFPG 420
           KNARKLKVCRYR++EDEF SP  TEL KYL DE++S A+GFYILLRAVDRFA  Y  FPG
Sbjct: 373 KNARKLKVCRYRTIEDEFKSPSTTELHKYLADENYSGAIGFYILLRAVDRFAGTYKKFPG 432

Query: 421 QFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSEDLINEMCRFGAAELHTVAAFIGGIASQ 480
           QFDG  DED S+LKT A+ LLS++GC+G  L E+L NEMCRFGAAE+H VAA IGGI SQ
Sbjct: 433 QFDGSTDEDASQLKTIALSLLSEMGCDGYELQEELYNEMCRFGAAEIHVVAALIGGITSQ 492

Query: 481 EVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 512
           EVIKLIT+QFVP  GT++FNGIDHKSQ L+L
Sbjct: 493 EVIKLITKQFVPKRGTFIFNGIDHKSQSLTL 523

BLAST of MC02g0458 vs. TAIR 10
Match: AT1G05180.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 652.9 bits (1683), Expect = 2.1e-187
Identity = 309/418 (73.92%), Postives = 371/418 (88.76%), Query Frame = 0

Query: 95  LIETRPSFFSQFTLVVATQLGEEWIVKLDRICRKANVILIVARSYGLTGLVRISLKEHTV 154
           L   +P+ F  FTLV+ATQL E+ ++KLDRICR ANV L++ RSYGL G VRIS+KEH +
Sbjct: 21  LANQKPNLF--FTLVIATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPI 80

Query: 155 IESKPDHFLDDLRLNNPWPELRRFAESIDVNVPDP-VAHKHIPYVVVLVKMAEEWSKSHG 214
           I+SKPDHFLDDLRLNNPWPEL+ F E+ID+NV +P  AHKHIPYVV+LVKMAEEW++SH 
Sbjct: 81  IDSKPDHFLDDLRLNNPWPELKSFVETIDLNVSEPAAAHKHIPYVVILVKMAEEWAQSHS 140

Query: 215 GSLPSSREEKKAFKDLLKAKMIAMDEDNYREAIEASFKVFTPHGISPDLKQIVDDSCAEV 274
           G+LPS+REEKK FKDL+K+KM++ DEDNY+EAIEA+FKVF P GIS +++++++DSCAEV
Sbjct: 141 GNLPSTREEKKEFKDLVKSKMVSTDEDNYKEAIEAAFKVFAPRGISSEVQKLINDSCAEV 200

Query: 275 DSNSSDFWILVAALKEFIVNEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVI 334
           +SNSS FW++VAALKEF++NEGGGEAP+EGSIPDMTSSTEHY+NLQ IY AKAEADF+VI
Sbjct: 201 NSNSSAFWVMVAALKEFVLNEGGGEAPLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVI 260

Query: 335 DQRARNILKKIGRDPNSISKTTTKSFCKNARKLKVCRYRSLEDEFNSPVFTELQKYLTDE 394
           ++R +NILKKIGRDP+SI K T KSFCKNARKLK+CRYR +EDEF +P  TE+QKYL DE
Sbjct: 261 EERVKNILKKIGRDPSSIPKPTIKSFCKNARKLKLCRYRMVEDEFRNPSVTEIQKYLADE 320

Query: 395 DFSVAVGFYILLRAVDRFAANYNSFPGQFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSE 454
           D+S A+GFYILLRA DRFAANYN FPGQFDGGMDEDISRLKTTA+ LL+DLGCNG  L +
Sbjct: 321 DYSGAMGFYILLRAADRFAANYNKFPGQFDGGMDEDISRLKTTALSLLTDLGCNGSVLPD 380

Query: 455 DLINEMCRFGAAELHTVAAFIGGIASQEVIKLITRQFVPMSGTYVFNGIDHKSQLLSL 512
           DLI+EMCRFGA+E+H V+AF+GGIASQEVIKL+T+QFVPM GTY+FNGIDHKSQLL L
Sbjct: 381 DLIHEMCRFGASEIHVVSAFVGGIASQEVIKLVTKQFVPMLGTYIFNGIDHKSQLLKL 436

BLAST of MC02g0458 vs. TAIR 10
Match: AT2G32410.2 (AXR1-like )

HSP 1 Score: 609.0 bits (1569), Expect = 3.5e-174
Identity = 289/399 (72.43%), Postives = 348/399 (87.22%), Query Frame = 0

Query: 1   RIWGEQGQAALEKASICLLNCGPTGSETLKNLVLGGIGSITIVDGTKVEEGDLGNNFMVD 60
           RIWGE GQ+ALE ASICLLNCGPTGSE LKNLV+GGIGSITIVDG+KVE GDLGNNFMVD
Sbjct: 13  RIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKVEIGDLGNNFMVD 72

Query: 61  ESSIGQSKAKCVCAFLQELNDAVKAKFIEEFPEALIETRPSFFSQFTLVVATQLGEEWIV 120
             S+GQS+AK VC FLQELND+VKA F+EE P+ LI T PSFFSQFTLV+ATQL E+ +V
Sbjct: 73  AKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTLVIATQLVEDSMV 132

Query: 121 KLDRICRKANVILIVARSYGLTGLVRISLKEHTVIESKPDHFLDDLRLNNPWPELRRFAE 180
           KLDRICR+ANV+L++ARSYGLTG VRIS+KEHT IE+KPDH LDDLRLN+PWPEL+ + E
Sbjct: 133 KLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRLNSPWPELKSYVE 192

Query: 181 SIDVNVPDPVAHKHIPYVVVLVKMAEEWSKSHGGSLPSSREEKKAFKDLLKAKMIAMDED 240
           SID+NV +P AHKHIPYVV+LVK+AEEW++ H G+LPS+REEK  FKDL+K+KM++ DE+
Sbjct: 193 SIDLNVEEPAAHKHIPYVVILVKVAEEWAQHHSGNLPSTREEKNEFKDLVKSKMVSADEE 252

Query: 241 NYREAIEASFKVFTPHGISPDLKQIVDDSCAEVDSNSSDFWILVAALKEFIVNEGGGEAP 300
           NY+EA+ A+FKVF P GIS +++ I  DSCAEV SNSSDFW++VAALKEFI NEGGGE P
Sbjct: 253 NYKEALLAAFKVFAPTGISQEIQDINHDSCAEVGSNSSDFWVMVAALKEFISNEGGGEVP 312

Query: 301 MEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVIDQRARNILKKIGRDPNSISKTTTKSFC 360
           +EGS+PDM SSTEHY+NLQ IY +KAEADF+ ++QR ++IL K+G+DP+SISK T KSFC
Sbjct: 313 LEGSMPDMISSTEHYINLQKIYHSKAEADFLSMEQRVKSILVKVGQDPSSISKPTIKSFC 372

Query: 361 KNARKLKVCRYRSLEDEFNSPVFTELQKYLTDEDFSVAV 400
           KNARKLKVCRYR++EDEF SP  TEL KYL DE++S ++
Sbjct: 373 KNARKLKVCRYRTIEDEFKSPSTTELHKYLADENYSCSI 411

BLAST of MC02g0458 vs. TAIR 10
Match: AT1G05180.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 606.3 bits (1562), Expect = 2.3e-173
Identity = 290/407 (71.25%), Postives = 355/407 (87.22%), Query Frame = 0

Query: 95  LIETRPSFFSQFTLVVATQLGEEWIVKLDRICRKANVILIVARSYGLTGLVRISLKEHTV 154
           L   +P+ F  FTLV+ATQL E+ ++KLDRICR ANV L++ RSYGL G VRIS+KEH +
Sbjct: 21  LANQKPNLF--FTLVIATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPI 80

Query: 155 IESKPDHFLDDLRLNNPWPELRRFAESIDVNVPDP-VAHKHIPYVVVLVKMAEEWSKSHG 214
           I+SKPDHFLDDLRLNNPWPEL+ F E+ID+NV +P  AHKHIPYVV+LVKMAEEW++SH 
Sbjct: 81  IDSKPDHFLDDLRLNNPWPELKSFVETIDLNVSEPAAAHKHIPYVVILVKMAEEWAQSHS 140

Query: 215 GSLPSSREEKKAFKDLLKAKMIAMDEDNYREAIEASFKVFTPHGISPDLKQIVDDSCAEV 274
           G+LPS+REEKK FKDL+K+KM++ DEDNY+EAIEA+FKVF P GIS +++++++DSCAEV
Sbjct: 141 GNLPSTREEKKEFKDLVKSKMVSTDEDNYKEAIEAAFKVFAPRGISSEVQKLINDSCAEV 200

Query: 275 DSNSSDFWILVAALKEFIVNEGGGEAPMEGSIPDMTSSTEHYVNLQNIYQAKAEADFMVI 334
           +SNSS FW++VAALKEF++NEGGGEAP+EGSIPDMTSSTEHY+NLQ IY AKAEADF+VI
Sbjct: 201 NSNSSAFWVMVAALKEFVLNEGGGEAPLEGSIPDMTSSTEHYINLQKIYLAKAEADFLVI 260

Query: 335 DQRARNILKKIGRDPNSISKTTTKSFCKNARKLKVCRYRSLEDEFNSPVFTELQKYLTDE 394
           ++R +NILKKIGRDP+SI K T KSFCKNARKLK+CRYR +EDEF +P  TE+QKYL DE
Sbjct: 261 EERVKNILKKIGRDPSSIPKPTIKSFCKNARKLKLCRYRMVEDEFRNPSVTEIQKYLADE 320

Query: 395 DFSVAVGFYILLRAVDRFAANYNSFPGQFDGGMDEDISRLKTTAVGLLSDLGCNGLTLSE 454
           D+S A+GFYILLRA DRFAANYN FPGQFDGGMDEDISRLKTTA+ LL+DLGCNG  L +
Sbjct: 321 DYSGAMGFYILLRAADRFAANYNKFPGQFDGGMDEDISRLKTTALSLLTDLGCNGSVLPD 380

Query: 455 DLINEMCRFGAAELHTVAAFIGGIASQEVIKLITRQFVPMSGTYVFN 501
           DLI+EMCRFGA+E+H V+AF+GGIASQEVIK+   +F+ +S  + F+
Sbjct: 381 DLIHEMCRFGASEIHVVSAFVGGIASQEVIKV---RFIFLSHCFKFS 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P427447.5e-23876.56NEDD8-activating enzyme E1 regulatory subunit AXR1 OS=Arabidopsis thaliana OX=37... [more]
Q9ZV692.1e-22472.80NEDD8-activating enzyme E1 regulatory subunit AXL OS=Arabidopsis thaliana OX=370... [more]
Q54JM33.5e-11842.94NEDD8-activating enzyme E1 regulatory subunit OS=Dictyostelium discoideum OX=446... [more]
Q6NTW61.3e-11741.12NEDD8-activating enzyme E1 regulatory subunit OS=Xenopus laevis OX=8355 GN=nae1 ... [more]
Q8VBW61.7e-11740.93NEDD8-activating enzyme E1 regulatory subunit OS=Mus musculus OX=10090 GN=Nae1 P... [more]
Match NameE-valueIdentityDescription
XP_022140497.10.0100.00NEDD8-activating enzyme E1 regulatory subunit AXR1 [Momordica charantia][more]
XP_023512938.10.095.30NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita pepo subsp. pepo][more]
XP_022944430.10.094.91NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita moschata] >KAG7010... [more]
XP_022986290.10.094.72NEDD8-activating enzyme E1 regulatory subunit AXR1 [Cucurbita maxima][more]
KAG6570885.10.094.85NEDD8-activating enzyme E1 regulatory subunit AXR1, partial [Cucurbita argyrospe... [more]
Match NameE-valueIdentityDescription
A0A6J1CH540.0100.00NEDD8-activating enzyme E1 regulatory subunit OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1FYN40.094.91NEDD8-activating enzyme E1 regulatory subunit OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JDN60.094.72NEDD8-activating enzyme E1 regulatory subunit OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0LMV70.093.74NEDD8-activating enzyme E1 regulatory subunit OS=Cucumis sativus OX=3659 GN=Csa_... [more]
A0A1S3C1I60.092.95NEDD8-activating enzyme E1 regulatory subunit OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT1G05180.15.3e-23976.56NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G32410.11.5e-22572.80AXR1-like [more]
AT1G05180.22.1e-18773.92NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G32410.23.5e-17472.43AXR1-like [more]
AT1G05180.32.3e-17371.25NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 3..490
e-value: 1.3E-21
score: 77.1
IPR030667NEDD8-activating enzyme E1 regulatory subunit APP-BP1PIRSFPIRSF039099APP-BP1coord: 1..511
e-value: 2.2E-168
score: 559.4
NoneNo IPR availableGENE3D3.40.50.720coord: 1..256
e-value: 4.7E-58
score: 198.8
coord: 348..511
e-value: 2.2E-36
score: 127.7
NoneNo IPR availablePANTHERPTHR10953:SF217NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT AXR1coord: 5..511
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 5..511
NoneNo IPR availableCDDcd01493APPBP1_RUBcoord: 1..509
e-value: 0.0
score: 596.594
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 1..507

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0458.1MC02g0458.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045116 protein neddylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0019781 NEDD8 activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity