MC02g0404 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0404
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	glucan endo-1,3-beta-glucosidase 1
Location	MC02: 3466060 .. 3466824 (+)
RNA-Seq Expression	MC02g0404
Synteny	MC02g0404

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGCTAATCTTGTATTGATTTTTGTCCAAAAATTATACCTTGCTAATCAAGATATCATTTTCAGTAATAAAATCACGATTTCCTGTACATCTAGTTCCATTCGATGCCATGGTTTTGATTGTACAAAACCGATGCCATTTTTCTTTTGAGGTATGGCCTAAGCCTACCCACTTGGAAGCCAATTCGAATTAATATATGCCATCTTTTGGGGAATATCAACTGTTTTTGCTTAATTAGTTGTAATCAAATTTGATAAATAGCAGCACATGGGCAACAGGGCAAGCCCTCTTTCTCGAATGCATCGCTCTCTCTCTGCCATGGCTGCTGTTTTGACAAGAACACTGCTAACTCTGCTATTACTGTCAACAATTCAGCAAAAAGCAGGTAACGACCAGAATCCACTTGAAATTTTCAAGTCAAAGAATGCTCAGTACAAATCTCCAAGTTTTCATTTCTACAATTTCTTGGTTTGATTATATTGTGCCCACCAAGTGTTTGTTAAAATGAGTAGGTGGGGAGTTTGAGCAATGGTGTGTAGCAGACGAGCAGAGCCCAGATGATGAATTGCAAATGGCTTTGGATTGGGCGTGTGGAGGAGGAGGTGCCAACTGCAGCAAGATCCAAGGAAACCAATCTTGCTTTTACCCAAATACAGTGAAAGACCATGCTTCATTTGCCTTCAACTCCTACTTCCAGAACTTCAAGCACCACGGTGGATCCTGCTTCTTCAAAGGAGCTGCCATCATTACTGAGACTGATCCTAGT

mRNA sequence

TGGCTAATCTTGTATTGATTTTTGTCCAAAAATTATACCTTGCTAATCAAGATATCATTTTCAGTAATAAAATCACGATTTCCTGTACATCTAGTTCCATTCGATGCCATGGTTTTGATTGTACAAAACCGATGCCATTTTTCTTTTGAGCAGCACATGGGCAACAGGGCAAGCCCTCTTTCTCGAATGCATCGCTCTCTCTCTGCCATGGCTGCTGTTTTGACAAGAACACTGCTAACTCTGCTATTACTGTCAACAATTCAGCAAAAAGCAGGTGGGGAGTTTGAGCAATGGTGTGTAGCAGACGAGCAGAGCCCAGATGATGAATTGCAAATGGCTTTGGATTGGGCGTGTGGAGGAGGAGGTGCCAACTGCAGCAAGATCCAAGGAAACCAATCTTGCTTTTACCCAAATACAGTGAAAGACCATGCTTCATTTGCCTTCAACTCCTACTTCCAGAACTTCAAGCACCACGGTGGATCCTGCTTCTTCAAAGGAGCTGCCATCATTACTGAGACTGATCCTAGT

Coding sequence (CDS)

ATGGTTTTGATTGTACAAAACCGATGCCATTTTTCTTTTGAGCAGCACATGGGCAACAGGGCAAGCCCTCTTTCTCGAATGCATCGCTCTCTCTCTGCCATGGCTGCTGTTTTGACAAGAACACTGCTAACTCTGCTATTACTGTCAACAATTCAGCAAAAAGCAGGTGGGGAGTTTGAGCAATGGTGTGTAGCAGACGAGCAGAGCCCAGATGATGAATTGCAAATGGCTTTGGATTGGGCGTGTGGAGGAGGAGGTGCCAACTGCAGCAAGATCCAAGGAAACCAATCTTGCTTTTACCCAAATACAGTGAAAGACCATGCTTCATTTGCCTTCAACTCCTACTTCCAGAACTTCAAGCACCACGGTGGATCCTGCTTCTTCAAAGGAGCTGCCATCATTACTGAGACTGATCCTAGT

Protein sequence

MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITETDPS

Homology

[Close]

BLAST of MC02g0404 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 98.6 bits (244), Expect = 6.0e-20
Identity = 46/81 (56.79%), Postives = 56/81 (69.14%), Query Frame = 0


Query: 60  EQWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNF 119
           + +C+A +      LQ ALDWACG G +NCS+IQ  +SC+ PN VK HASFAFNSY+Q  
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 120 KHHGGSCFFKGAAIITETDPS 141
               GSC FKG A+IT TDPS
Sbjct: 439 GRASGSCDFKGVAMITTTDPS 459

[Close]

BLAST of MC02g0404 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 3.9e-19
Identity = 44/79 (55.70%), Postives = 52/79 (65.82%), Query Frame = 0


Query: 62  WCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKH 121
           +CVA   + DD+L   L+WACG G ANC+ IQ  Q C+ PN VK HASFAFN Y+Q  K 
Sbjct: 362 FCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKS 421

Query: 122 HGGSCFFKGAAIITETDPS 141
            GG+C F G AI T  DPS
Sbjct: 422 AGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of MC02g0404 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 4.3e-18
Identity = 46/123 (37.40%), Postives = 68/123 (55.28%), Query Frame = 0


Query: 18  GNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQMA 77
           G   +P+S  + +     +  +  ++      TI    GG  ++WC+A  Q+   ELQ A
Sbjct: 347 GENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTA 406

Query: 78  LDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITET 137
           LDWACG G  +CS +Q +Q CF P+TV  HAS+AFN+Y+Q        C F GA++  + 
Sbjct: 407 LDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDK 466

Query: 138 DPS 141
           DPS
Sbjct: 467 DPS 469

[Close]

BLAST of MC02g0404 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 8.0e-17
Identity = 38/86 (44.19%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 55  AGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNS 114
           + G    WC+A  ++ + +L+ ALDWACG G  +C+ IQ +Q CF P+T+  HASF FNS
Sbjct: 362 SSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNS 421

Query: 115 YFQNFKHHGGSCFFKGAAIITETDPS 141
           YFQ  +    +C F GA +    DPS
Sbjct: 422 YFQQNRATDVACSFGGAGVKVNKDPS 447

[Close]

BLAST of MC02g0404 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-15
Identity = 37/81 (45.68%), Postives = 52/81 (64.20%), Query Frame = 0


Query: 60  EQWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNF 119
           + +C+A E+     LQ ALDWACG G  +CS +   +SC+ P+ V  H+++AFN+Y+Q  
Sbjct: 358 QTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKM 417

Query: 120 KHHGGSCFFKGAAIITETDPS 141
               GSC FKG A +T TDPS
Sbjct: 418 GKASGSCDFKGVATVTTTDPS 438

[Close]

BLAST of MC02g0404 vs. NCBI nr
Match: XP_022140326.1 (glucan endo-1,3-beta-glucosidase 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 290 bits (741), Expect = 1.12e-98
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE 60
           MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE
Sbjct: 1   MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE 60

Query: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120
           QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK
Sbjct: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120

Query: 121 HHGGSCFFKGAAIITETDPS 140
           HHGGSCFFKGAAIITETDPS
Sbjct: 121 HHGGSCFFKGAAIITETDPS 140

[Close]

BLAST of MC02g0404 vs. NCBI nr
Match: XP_022140327.1 (glucan endo-1,3-beta-glucosidase 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 283 bits (724), Expect = 4.25e-96
Identity = 139/140 (99.29%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE 60
           MVLIVQNRCHFSFE HMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE
Sbjct: 1   MVLIVQNRCHFSFE-HMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE 60

Query: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120
           QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK
Sbjct: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120

Query: 121 HHGGSCFFKGAAIITETDPS 140
           HHGGSCFFKGAAIITETDPS
Sbjct: 121 HHGGSCFFKGAAIITETDPS 139

[Close]

BLAST of MC02g0404 vs. NCBI nr
Match: XP_022140328.1 (glucan endo-1,3-beta-glucosidase 1-like isoform X3 [Momordica charantia])

HSP 1 Score: 256 bits (654), Expect = 1.21e-85
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0


Query: 17  MGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQM 76
           MGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQM
Sbjct: 1   MGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQM 60

Query: 77  ALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITE 136
           ALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITE
Sbjct: 61  ALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITE 120

Query: 137 TDPS 140
           TDPS
Sbjct: 121 TDPS 124

[Close]

BLAST of MC02g0404 vs. NCBI nr
Match: XP_004140010.1 (glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis sativus])

HSP 1 Score: 190 bits (482), Expect = 1.15e-59
Identity = 90/107 (84.11%), Postives = 95/107 (88.79%), Query Frame = 0


Query: 34  MAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSKIQ 93
           M A+LT+TLL LLLLSTI Q  GGEFEQWCVADEQ+PDDELQMALDWACG GGANCS IQ
Sbjct: 1   MVALLTKTLLVLLLLSTIHQNTGGEFEQWCVADEQTPDDELQMALDWACGRGGANCSSIQ 60

Query: 94  GNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITETDPS 140
            NQ CF PNTVKDHASFAFN+YFQ+FKH GGSCFFKGAAIITE DPS
Sbjct: 61  PNQPCFNPNTVKDHASFAFNNYFQSFKHQGGSCFFKGAAIITELDPS 107

[Close]

BLAST of MC02g0404 vs. NCBI nr
Match: XP_022947538.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata] >XP_022971134.1 glucan endo-1,3-beta-glucosidase 12 [Cucurbita maxima] >XP_023532863.1 glucan endo-1,3-beta-glucosidase 12-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 190 bits (482), Expect = 1.23e-59
Identity = 93/109 (85.32%), Postives = 97/109 (88.99%), Query Frame = 0


Query: 34  MAAVLTRTLLTL--LLLSTIQQKAGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSK 93
           MAA+ T TLLTL  LLLSTIQ KAGGEFEQWC+ADEQ+PDDELQMALDWACG GG NCSK
Sbjct: 1   MAALWTGTLLTLALLLLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60

Query: 94  IQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITETDPS 140
           IQ NQ CF PNTVKDHASFAFN+YFQNFKH GGSCFFKGAAIITE DPS
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 109

[Close]

BLAST of MC02g0404 vs. ExPASy TrEMBL
Match: A0A6J1CFS1 (glucan endo-1,3-beta-glucosidase 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011027 PE=4 SV=1)

HSP 1 Score: 290 bits (741), Expect = 5.44e-99
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE 60
           MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE
Sbjct: 1   MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE 60

Query: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120
           QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK
Sbjct: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120

Query: 121 HHGGSCFFKGAAIITETDPS 140
           HHGGSCFFKGAAIITETDPS
Sbjct: 121 HHGGSCFFKGAAIITETDPS 140

[Close]

BLAST of MC02g0404 vs. ExPASy TrEMBL
Match: A0A6J1CGI9 (glucan endo-1,3-beta-glucosidase 1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011027 PE=4 SV=1)

HSP 1 Score: 283 bits (724), Expect = 2.06e-96
Identity = 139/140 (99.29%), Postives = 139/140 (99.29%), Query Frame = 0


Query: 1   MVLIVQNRCHFSFEQHMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE 60
           MVLIVQNRCHFSFE HMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE
Sbjct: 1   MVLIVQNRCHFSFE-HMGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFE 60

Query: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120
           QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK
Sbjct: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120

Query: 121 HHGGSCFFKGAAIITETDPS 140
           HHGGSCFFKGAAIITETDPS
Sbjct: 121 HHGGSCFFKGAAIITETDPS 139

[Close]

BLAST of MC02g0404 vs. ExPASy TrEMBL
Match: A0A6J1CHS0 (glucan endo-1,3-beta-glucosidase 1-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011027 PE=4 SV=1)

HSP 1 Score: 256 bits (654), Expect = 5.87e-86
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0


Query: 17  MGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQM 76
           MGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQM
Sbjct: 1   MGNRASPLSRMHRSLSAMAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQM 60

Query: 77  ALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITE 136
           ALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITE
Sbjct: 61  ALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITE 120

Query: 137 TDPS 140
           TDPS
Sbjct: 121 TDPS 124

[Close]

BLAST of MC02g0404 vs. ExPASy TrEMBL
Match: A0A0A0KDX6 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G110840 PE=4 SV=1)

HSP 1 Score: 190 bits (482), Expect = 5.58e-60
Identity = 90/107 (84.11%), Postives = 95/107 (88.79%), Query Frame = 0


Query: 34  MAAVLTRTLLTLLLLSTIQQKAGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSKIQ 93
           M A+LT+TLL LLLLSTI Q  GGEFEQWCVADEQ+PDDELQMALDWACG GGANCS IQ
Sbjct: 1   MVALLTKTLLVLLLLSTIHQNTGGEFEQWCVADEQTPDDELQMALDWACGRGGANCSSIQ 60

Query: 94  GNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITETDPS 140
            NQ CF PNTVKDHASFAFN+YFQ+FKH GGSCFFKGAAIITE DPS
Sbjct: 61  PNQPCFNPNTVKDHASFAFNNYFQSFKHQGGSCFFKGAAIITELDPS 107

[Close]

BLAST of MC02g0404 vs. ExPASy TrEMBL
Match: A0A6J1I7Q3 (glucan endo-1,3-beta-glucosidase 12 OS=Cucurbita maxima OX=3661 GN=LOC111469899 PE=4 SV=1)

HSP 1 Score: 190 bits (482), Expect = 5.95e-60
Identity = 93/109 (85.32%), Postives = 97/109 (88.99%), Query Frame = 0


Query: 34  MAAVLTRTLLTL--LLLSTIQQKAGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSK 93
           MAA+ T TLLTL  LLLSTIQ KAGGEFEQWC+ADEQ+PDDELQMALDWACG GG NCSK
Sbjct: 1   MAALWTGTLLTLALLLLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60

Query: 94  IQGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITETDPS 140
           IQ NQ CF PNTVKDHASFAFN+YFQNFKH GGSCFFKGAAIITE DPS
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 109

[Close]

BLAST of MC02g0404 vs. TAIR 10
Match: AT5G35740.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 7.2e-37
Identity = 71/109 (65.14%), Postives = 90/109 (82.57%), Query Frame = 0


Query: 34  MAAVLTRTLLTLLLLS-TIQQKAGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSKI 93
           M+  L R LL LL+++ T+ +++  E EQWC+ADEQ+PDDELQ ALDWACG GGA+CSK+
Sbjct: 1   MSTFLIRILLPLLIIAMTLPRRSEAESEQWCIADEQTPDDELQAALDWACGKGGADCSKM 60

Query: 94  -QGNQSCFYPNTVKDHASFAFNSYFQNFKHHGGSCFFKGAAIITETDPS 141
            Q NQ CF PNT++DHASFAFNSY+Q +K+ GGSC+FKGAA+ITE DPS
Sbjct: 61  QQENQPCFLPNTIRDHASFAFNSYYQTYKNKGGSCYFKGAAMITELDPS 109

[Close]

BLAST of MC02g0404 vs. TAIR 10
Match: AT2G04910.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.2e-28
Identity = 55/86 (63.95%), Postives = 64/86 (74.42%), Query Frame = 0


Query: 55  AGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNS 114
           A  EF QWCVAD Q PD+ +Q A+DWAC  GGA+CS IQ NQ CF PNTVKDHAS  FN+
Sbjct: 8   AKAEFGQWCVADGQIPDNVIQAAVDWACQTGGADCSTIQPNQPCFLPNTVKDHASVVFNN 67

Query: 115 YFQNFKHHGGSCFFKGAAIITETDPS 141
           Y+Q +K +GGSC F   A IT+TDPS
Sbjct: 68  YYQRYKRNGGSCNFNSTAFITQTDPS 93

[Close]

BLAST of MC02g0404 vs. TAIR 10
Match: AT2G04910.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.2e-28
Identity = 55/86 (63.95%), Postives = 64/86 (74.42%), Query Frame = 0


Query: 55  AGGEFEQWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNS 114
           A  EF QWCVAD Q PD+ +Q A+DWAC  GGA+CS IQ NQ CF PNTVKDHAS  FN+
Sbjct: 8   AKAEFGQWCVADGQIPDNVIQAAVDWACQTGGADCSTIQPNQPCFLPNTVKDHASVVFNN 67

Query: 115 YFQNFKHHGGSCFFKGAAIITETDPS 141
           Y+Q +K +GGSC F   A IT+TDPS
Sbjct: 68  YYQRYKRNGGSCNFNSTAFITQTDPS 93

[Close]

BLAST of MC02g0404 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 4.2e-21
Identity = 47/81 (58.02%), Postives = 59/81 (72.84%), Query Frame = 0


Query: 61  QWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNFK 120
           QWC+A   +    LQ+ALD+ACG GGA+C +IQ   +C+ PNT++DHASFAFNSY+Q  K
Sbjct: 147 QWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQ--K 206

Query: 121 HHGG-SCFFKGAAIITETDPS 141
           H G  SC F GAA +T TDPS
Sbjct: 207 HPGSDSCNFGGAAQLTSTDPS 225

[Close]

BLAST of MC02g0404 vs. TAIR 10
Match: AT1G11820.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 98.6 bits (244), Expect = 4.2e-21
Identity = 46/81 (56.79%), Postives = 56/81 (69.14%), Query Frame = 0


Query: 60  EQWCVADEQSPDDELQMALDWACGGGGANCSKIQGNQSCFYPNTVKDHASFAFNSYFQNF 119
           + +C+A +      LQ ALDWACG G +NCS+IQ  +SC+ PN VK HASFAFNSY+Q  
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 120 KHHGGSCFFKGAAIITETDPS 141
               GSC FKG A+IT TDPS
Sbjct: 439 GRASGSCDFKGVAMITTTDPS 459

The following BLAST results are available for this feature:

BLAST of MC02g0404 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65399	6.0e-20	56.79	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q94CD8	3.9e-19	55.70	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q8VYE5	4.3e-18	37.40	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	8.0e-17	44.19	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q9ZU91	1.2e-15	45.68	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]

BLAST of MC02g0404 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140326.1	1.12e-98	100.00	glucan endo-1,3-beta-glucosidase 1-like isoform X1 [Momordica charantia]	[more]
XP_022140327.1	4.25e-96	99.29	glucan endo-1,3-beta-glucosidase 1-like isoform X2 [Momordica charantia]	[more]
XP_022140328.1	1.21e-85	100.00	glucan endo-1,3-beta-glucosidase 1-like isoform X3 [Momordica charantia]	[more]
XP_004140010.1	1.15e-59	84.11	glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis sativus]	[more]
XP_022947538.1	1.23e-59	85.32	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata] >XP_022971134.1 gl...	[more]

BLAST of MC02g0404 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CFS1	5.44e-99	100.00	glucan endo-1,3-beta-glucosidase 1-like isoform X1 OS=Momordica charantia OX=367...	[more]
A0A6J1CGI9	2.06e-96	99.29	glucan endo-1,3-beta-glucosidase 1-like isoform X2 OS=Momordica charantia OX=367...	[more]
A0A6J1CHS0	5.87e-86	100.00	glucan endo-1,3-beta-glucosidase 1-like isoform X3 OS=Momordica charantia OX=367...	[more]
A0A0A0KDX6	5.58e-60	84.11	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G110840 PE=4 SV=...	[more]
A0A6J1I7Q3	5.95e-60	85.32	glucan endo-1,3-beta-glucosidase 12 OS=Cucurbita maxima OX=3661 GN=LOC111469899 ...	[more]

BLAST of MC02g0404 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35740.1	7.2e-37	65.14	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G04910.1	1.2e-28	63.95	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G04910.2	1.2e-28	63.95	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G29380.1	4.2e-21	58.02	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G11820.2	4.2e-21	56.79	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 61..140 e-value: 6.1E-37 score: 138.7
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 61..132 e-value: 6.6E-21 score: 74.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 58..140 e-value: 1.0E-10 score: 43.9
None	No IPR available	PANTHER	PTHR31044:SF35	CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEIN	coord: 37..140
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 37..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g0404.1	MC02g0404.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0016787	hydrolase activity