MC02g0334 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0334
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionrop guanine nucleotide exchange factor 1
LocationMC02: 2943937 .. 2947067 (-)
RNA-Seq ExpressionMC02g0334
SyntenyMC02g0334
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAAACAAAAAAAAACAACCGCCGTTTCCAGTGTCACAAGTAATTCCCTCCAAATCCAACGGTAAATCGACGGTGAAGGTTCTCCGGGACCCACTTGGGCAGAGTCCACGGTGCATGATCGTGTCCACGTCATCGCTCGCAGTCCAGCCTGGCGAAACGGACTGTGGAATTACGGTGGGCCCACAGGGAAAGGCCGTGTCGGATGCGAAGACCTACTGTTACGGAAAGACAAAGCTGTCCAAACGGCGTCGCTTTTGCTTTTCCTCTGCCTCATCATCTTCACACAGAGAGAGGAGAGAGAAAGAAGAGAGAGAGAGCTCAGTGTGAGTGAGAACCTGAGCGAGGGGCCATATATGCTTCTTTTTTGTTCGCTTTATCAATTATCGCTTCCTCTTCATCTCTCCTGCAAGTTTTTTCTTCTGGGTTTCAGACGACCACGCCATCCATTGTTCTTTTTCTGGTCTTCTGCATCGACGAATGTGCGTCTGAGGGGTTTCTTTTGCTTTTCCGGCGATGGGTAGTGTGTCTTCCGATGACGGCTCCGACCAGCTGAGCGAGCGGTGCGGGAGCTACAGTTTGAGTGCGGATGTGAGCGAGTCCGAGAGTTGCAGTAGCTTCTCCTGCCGGAGGTTTGATGGCGAAGGGGCTTCAAGCTCAATGGCGTCGTCTCCGCATCCTGTTTCCGGGAATTTCTGCTTTCCGCCGCCGGTTTCGTTGCCGGTCATTGGAGGCAAGGATGTTTTTGTTTGGGATGACAAGTCTAAGAAGCGGGACGCTGATTTATCTGGTAATTTTATTTCTCTTGTGGGTTTGTTTTTAGTGAGAAAATGGATTTTGTTTCAGTATCTGTCGCCAGTCGGTCGATCTCTTTGTGTAATTCGACAATGTGGCAAGATTTTAGGCATTGGAGGGTTTTTTTTGCAAGAGTAATTCGATTCCCTCTGTCTTGCTAAATTTCTGAGATAATTGAGAGAACTATTACGATGGTGTTTTCTCCCCTTCTTTTTCTTTCAGAGATTTAGCTGCATAAACAGATCAGAGAGTGTACTGTAGGATGAGAACTGAACTGTGGTTTCTTCTTGCTTCCTGACGGCGAAGCAGAGAAACCATCCATTTTTTTAATGAGAGATCTCATCTATTGTGGAATAGGTTAAAACGGCGGTTTACCATCTTTAGCCTCTGATCTCTCATTTTTATTTTATGCAGAAGTTGAGATGATGAAGGAGAGGTTTGCGAAGCTCCTCCTTGGGGAAGACATGTCAGGGGGAGGGAAAGGGGTCTGTACCGCCCTTGCTATCTCAAATGCCATCACCAATCTCTCTGGTAGATCTTTATTTTGAGCCCCTTTAAGTGATTGTAAATTCGTAACTGTGTATTTCTCCGATTGCGTGAAGTTTAAGAACATTTCTGATAATGTTACAACCATTTTGGTCTGAAATTACAGCTACTGTATTTGGTGAATTGTGGAGATTGGAGCCACTGGCACCACAGAAAAGGGCAATGTGGCATAGAGAAATGGAATGGCTTCTATGCGTTTGTGATTCCATTGTGGAATTGGTTCCTACAGTTCAGCCTTTCCCAGGTGGAGGCACGTATGAAGTGATGATGTCGAAACCGCGCTCGGACTTGTATATGAACCTCCCTGCCCTGAAGAAGCTTGACGCAATGCTTCTCAGCATGCTCGATGGATTTTGCCATACGGAATTTTGGTATGTCGACCGGGGGATAGTTGTGGGTGACTTGAATGACTGTAATGGTTTCTTGCCCGGTGGGAGGCCTTCGATCAGGCAAGAAGATAAGTGGTGGCTTCCATGCCCAAAAGTTCCAGCAGATGGTCTGTCTGAAGATGCGAGAAAGAGGCTGCAGCAGTGCAGGGACTGTACTAATCAAATACTCAAAGCAGCCATGGCAATCAACAGCAATGTACTTGCTGAAATGGAAATTCCTACTGCCTACAAGGAAACCTTGCCCAAGGTATGGGTTTCTTGTCTGTTGACTGGCAATTCACTGAACTTTTGATGTTATCTTCCACGTATATCGATCTGCTGCTGAAGTTCTTTTTGCTGGTTGCTGTTCTCCATTTGCATGATTTAATCTTTTCTAAGAATCTATGTTTCTTGATCTGTTATACATGCTTCAAATGGTCCTGTTAATGTTAATGGGTTTCATATTTACATCGTCGTGCCACCTCTGGAATTCATTGCCGTGCCACCCCTGGAATTCATTGTTCATCAGATTGGAAAACAGATTCATGATGATTAAGTTCCTTTAGTTTTCTATCGTTATGATGAATGCAGAGCGGAAAAGCTTGCTTGGGAGATATCATTTATCGCTACATAACCGCTGACCACTTCTTGCCCGAATGCCTCCTTGATTGCTTGGACCTTTCATCTGAGCATCACACCGTGGAAGTAGCGAATAGGATCGAGGCTTCAGTTCATATTTGGAGGGCAAAAGACCATAAAAGAAGTGGTCACCGCAGTAAGGGAAGACGGCCAAATTGGAGTGGCAAGGTCAAGGGAATTGTTGCAGATTCGGGGAAGAGTCACAACCTGGCCCGGAGAGCGGAGACGCTTCTCGAAAGTCTAAGACTAAGATTTCCAGGATTACCACAGACAGGATTGGACATGGCCAAGATCCAATATAACAAAGTAATCCTTTCAATCCCTTTGGTTGTCTTTATCAACTGGAGTCAAGGGCTTGTCTAATGCCAAAAACTTTCCTGCAGGATGTGGGGCAGTCAATTCTGGAGAGCTATTCAAGGGTGATGGAGAGTTTAGCATTCAACATCATGGCACGTATTGACGACGTTCTATACGTAGATGATGCCATAAAGCGGTGCGTTGAAGCAGAGACAATCTCCCTTTTCAACCGAGGCTTGGGCGGTCTTCCCATCCAGAAACGCATGTCTCCCAGCCCCTTCTCAATCCACCACAGCCCATTTGCCTCCCCATTTGCAACTCCAACATTCTGTTCCTCGACTCCCATCGGCGGAAGCCCAGGAAGAGCATCCCCAACTGTAAAAAGAAGCAATGTCAAAGAAGCAGGCCAAGCTGACAAGGAGAGGATTTGGTCATACGCAGGGAATCTGAGTGGTAGAAGAATCTCTGGGGTTACTCCAGAGCGAGAC

mRNA sequence

CCCAAACAAAAAAAAACAACCGCCGTTTCCAGTGTCACAAGTAATTCCCTCCAAATCCAACGGTAAATCGACGGTGAAGGTTCTCCGGGACCCACTTGGGCAGAGTCCACGGTGCATGATCGTGTCCACGTCATCGCTCGCAGTCCAGCCTGGCGAAACGGACTGTGGAATTACGGTGGGCCCACAGGGAAAGGCCGTGTCGGATGCGAAGACCTACTGTTACGGAAAGACAAAGCTGTCCAAACGGCGTCGCTTTTGCTTTTCCTCTGCCTCATCATCTTCACACAGAGAGAGGAGAGAGAAAGAAGAGAGAGAGAGCTCAGTGTGAGTGAGAACCTGAGCGAGGGGCCATATATGCTTCTTTTTTGTTCGCTTTATCAATTATCGCTTCCTCTTCATCTCTCCTGCAAGTTTTTTCTTCTGGGTTTCAGACGACCACGCCATCCATTGTTCTTTTTCTGGTCTTCTGCATCGACGAATGTGCGTCTGAGGGGTTTCTTTTGCTTTTCCGGCGATGGGTAGTGTGTCTTCCGATGACGGCTCCGACCAGCTGAGCGAGCGGTGCGGGAGCTACAGTTTGAGTGCGGATGTGAGCGAGTCCGAGAGTTGCAGTAGCTTCTCCTGCCGGAGGTTTGATGGCGAAGGGGCTTCAAGCTCAATGGCGTCGTCTCCGCATCCTGTTTCCGGGAATTTCTGCTTTCCGCCGCCGGTTTCGTTGCCGGTCATTGGAGGCAAGGATGTTTTTGTTTGGGATGACAAGTCTAAGAAGCGGGACGCTGATTTATCTGAAGTTGAGATGATGAAGGAGAGGTTTGCGAAGCTCCTCCTTGGGGAAGACATGTCAGGGGGAGGGAAAGGGGTCTGTACCGCCCTTGCTATCTCAAATGCCATCACCAATCTCTCTGCTACTGTATTTGGTGAATTGTGGAGATTGGAGCCACTGGCACCACAGAAAAGGGCAATGTGGCATAGAGAAATGGAATGGCTTCTATGCGTTTGTGATTCCATTGTGGAATTGGTTCCTACAGTTCAGCCTTTCCCAGGTGGAGGCACGTATGAAGTGATGATGTCGAAACCGCGCTCGGACTTGTATATGAACCTCCCTGCCCTGAAGAAGCTTGACGCAATGCTTCTCAGCATGCTCGATGGATTTTGCCATACGGAATTTTGGTATGTCGACCGGGGGATAGTTGTGGGTGACTTGAATGACTGTAATGGTTTCTTGCCCGGTGGGAGGCCTTCGATCAGGCAAGAAGATAAGTGGTGGCTTCCATGCCCAAAAGTTCCAGCAGATGGTCTGTCTGAAGATGCGAGAAAGAGGCTGCAGCAGTGCAGGGACTGTACTAATCAAATACTCAAAGCAGCCATGGCAATCAACAGCAATGTACTTGCTGAAATGGAAATTCCTACTGCCTACAAGGAAACCTTGCCCAAGAGCGGAAAAGCTTGCTTGGGAGATATCATTTATCGCTACATAACCGCTGACCACTTCTTGCCCGAATGCCTCCTTGATTGCTTGGACCTTTCATCTGAGCATCACACCGTGGAAGTAGCGAATAGGATCGAGGCTTCAGTTCATATTTGGAGGGCAAAAGACCATAAAAGAAGTGGTCACCGCAGTAAGGGAAGACGGCCAAATTGGAGTGGCAAGGTCAAGGGAATTGTTGCAGATTCGGGGAAGAGTCACAACCTGGCCCGGAGAGCGGAGACGCTTCTCGAAAGTCTAAGACTAAGATTTCCAGGATTACCACAGACAGGATTGGACATGGCCAAGATCCAATATAACAAAGTAATCCTTTCAATCCCTTTGGTTGTCTTTATCAACTGGAGTCAAGGGTGTCTAATGCCAAAAACTTTCCTGCAGGATGTGGGGCAGTCAATTCTGGAGAGCTATTCAAGGGTGATGGAGAGTTTAGCATTCAACATCATGGCACGTATTGACGACGTTCTATACGTAGATGATGCCATAAAGCGGTGCGTTGAAGCAGAGACAATCTCCCTTTTCAACCGAGGCTTGGGCGGTCTTCCCATCCAGAAACGCATGTCTCCCAGCCCCTTCTCAATCCACCACAGCCCATTTGCCTCCCCATTTGCAACTCCAACATTCTGTTCCTCGACTCCCATCGGCGGAAGCCCAGGAAGAGCATCCCCAACTGTAAAAAGAAGCAATGTCAAAGAAGCAGGCCAAGCTGACAAGGAGAGGATTTGGTCATACGCAGGGAATCTGAGTGGTAGAAGAATCTCTGGGGTTACTCCAGAGCGAGAC

Coding sequence (CDS)

ATGGGTAGTGTGTCTTCCGATGACGGCTCCGACCAGCTGAGCGAGCGGTGCGGGAGCTACAGTTTGAGTGCGGATGTGAGCGAGTCCGAGAGTTGCAGTAGCTTCTCCTGCCGGAGGTTTGATGGCGAAGGGGCTTCAAGCTCAATGGCGTCGTCTCCGCATCCTGTTTCCGGGAATTTCTGCTTTCCGCCGCCGGTTTCGTTGCCGGTCATTGGAGGCAAGGATGTTTTTGTTTGGGATGACAAGTCTAAGAAGCGGGACGCTGATTTATCTGAAGTTGAGATGATGAAGGAGAGGTTTGCGAAGCTCCTCCTTGGGGAAGACATGTCAGGGGGAGGGAAAGGGGTCTGTACCGCCCTTGCTATCTCAAATGCCATCACCAATCTCTCTGCTACTGTATTTGGTGAATTGTGGAGATTGGAGCCACTGGCACCACAGAAAAGGGCAATGTGGCATAGAGAAATGGAATGGCTTCTATGCGTTTGTGATTCCATTGTGGAATTGGTTCCTACAGTTCAGCCTTTCCCAGGTGGAGGCACGTATGAAGTGATGATGTCGAAACCGCGCTCGGACTTGTATATGAACCTCCCTGCCCTGAAGAAGCTTGACGCAATGCTTCTCAGCATGCTCGATGGATTTTGCCATACGGAATTTTGGTATGTCGACCGGGGGATAGTTGTGGGTGACTTGAATGACTGTAATGGTTTCTTGCCCGGTGGGAGGCCTTCGATCAGGCAAGAAGATAAGTGGTGGCTTCCATGCCCAAAAGTTCCAGCAGATGGTCTGTCTGAAGATGCGAGAAAGAGGCTGCAGCAGTGCAGGGACTGTACTAATCAAATACTCAAAGCAGCCATGGCAATCAACAGCAATGTACTTGCTGAAATGGAAATTCCTACTGCCTACAAGGAAACCTTGCCCAAGAGCGGAAAAGCTTGCTTGGGAGATATCATTTATCGCTACATAACCGCTGACCACTTCTTGCCCGAATGCCTCCTTGATTGCTTGGACCTTTCATCTGAGCATCACACCGTGGAAGTAGCGAATAGGATCGAGGCTTCAGTTCATATTTGGAGGGCAAAAGACCATAAAAGAAGTGGTCACCGCAGTAAGGGAAGACGGCCAAATTGGAGTGGCAAGGTCAAGGGAATTGTTGCAGATTCGGGGAAGAGTCACAACCTGGCCCGGAGAGCGGAGACGCTTCTCGAAAGTCTAAGACTAAGATTTCCAGGATTACCACAGACAGGATTGGACATGGCCAAGATCCAATATAACAAAGTAATCCTTTCAATCCCTTTGGTTGTCTTTATCAACTGGAGTCAAGGGTGTCTAATGCCAAAAACTTTCCTGCAGGATGTGGGGCAGTCAATTCTGGAGAGCTATTCAAGGGTGATGGAGAGTTTAGCATTCAACATCATGGCACGTATTGACGACGTTCTATACGTAGATGATGCCATAAAGCGGTGCGTTGAAGCAGAGACAATCTCCCTTTTCAACCGAGGCTTGGGCGGTCTTCCCATCCAGAAACGCATGTCTCCCAGCCCCTTCTCAATCCACCACAGCCCATTTGCCTCCCCATTTGCAACTCCAACATTCTGTTCCTCGACTCCCATCGGCGGAAGCCCAGGAAGAGCATCCCCAACTGTAAAAAGAAGCAATGTCAAAGAAGCAGGCCAAGCTGACAAGGAGAGGATTTGGTCATACGCAGGGAATCTGAGTGGTAGAAGAATCTCTGGGGTTACTCCAGAGCGAGAC

Protein sequence

MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNFCFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRASPTVKRSNVKEAGQADKERIWSYAGNLSGRRISGVTPERD
Homology
BLAST of MC02g0334 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 734.6 bits (1895), Expect = 8.9e-211
Identity = 391/589 (66.38%), Postives = 455/589 (77.25%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSG-N 60
           MGS+SS++  +  SERCGSYS SAD+SESES SSFSC RFDGEGASSS+ SSP  V+G  
Sbjct: 1   MGSLSSEEDDEVSSERCGSYSPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVAGRG 60

Query: 61  FCFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           F FP PV LPVIGGKDV VWDD  K+ D DLSE+EMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FYFPAPVMLPVIGGKDV-VWDD--KQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSATVFGELWRLEPLAPQK+AMW RE+EWLLCV DSIVEL+P++Q FPGGG
Sbjct: 121 LAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGG 180

Query: 181 TYEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPG 240
           TYE+M ++PRSDLY NLPALKKLDAML+ MLD F  TEFWY DRGIV+G   DC+     
Sbjct: 181 TYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLG---DCDKDSYN 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPT 300
              S+RQEDKWWLPCPKVP +GLSE+ARK+LQQCRD  NQILKAA+AINS VLAEMEIP 
Sbjct: 241 SPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPD 300

Query: 301 AYKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRA 360
            Y ETLPKSGK CLG+IIY+Y+TA+ F PECLLDCLDLSSEH T+E+ANRIEA+VH+WR 
Sbjct: 301 PYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQ 360

Query: 361 KDHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMA 420
           K+ +R   ++K +  +W GKVKG+V D+ ++  L +RAETLL+SLR+RFPGLPQT LDM 
Sbjct: 361 KNGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMN 420

Query: 421 KIQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVL 480
           KIQYNK                         DVGQSILESYSRVMES+AFNI ARIDDVL
Sbjct: 421 KIQYNK-------------------------DVGQSILESYSRVMESMAFNITARIDDVL 480

Query: 481 YVDDAIKRCVE-AETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIG 540
           YVDDA++R +   E++SLF+  + GL  QK      FS+  SP  SPFATP    ++   
Sbjct: 481 YVDDAMRRSISVTESLSLFS--INGLNPQK-----AFSVQSSPHGSPFATPALSVAS--- 540

Query: 541 GSPGRASP--TVKRSNVKEAG-QADKERIWSYAGNLSGRRISGVTPERD 585
            SP RA P  +VKR+  +E G   + E+ WSYAGNLS RR++GVTPERD
Sbjct: 541 RSPRRAPPLYSVKRNGTREKGIVGETEKAWSYAGNLSSRRVTGVTPERD 548

BLAST of MC02g0334 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 7.5e-133
Identity = 270/532 (50.75%), Postives = 348/532 (65.41%), Query Frame = 0

Query: 28  ESESCSSFSCRRFDGEGASSSMAS-SPHPVSGNFCFPPPVSLPVIGGKDVFVWDDKSKKR 87
           ES  CSS + R+ + E + S     +  PVS  +      S+  I G      + K  + 
Sbjct: 17  ESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRW------SVKNIDG------EKKKIRS 76

Query: 88  DADLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQ 147
           D+ +SEVEMMKERF+KLLLGEDMSG G GVCTALAISNAITNL AT+FG+LWRLEPL  +
Sbjct: 77  DSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTE 136

Query: 148 KRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLYMNLPALKKLDAML 207
           K+ MW REMEWLLCV D IVE+ PT Q FP G   E+M  +PRSDLY+NLPAL+KLD ML
Sbjct: 137 KKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNML 196

Query: 208 LSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSIRQEDKWWLPCPKVPADGLSEDA 267
           L +LD F  TEFWYVD+GI+  + +  +G     +   RQEDKWWLP P+V   GL E++
Sbjct: 197 LEILDSFEETEFWYVDQGIMAHE-SAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENS 256

Query: 268 RKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKSGKACLGDIIYRYITADHF 327
           RK+LQ  RDCTNQILKAAMAINS  LA+MEIP +Y E+LP+ G++CLGD+IYRYI++D F
Sbjct: 257 RKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQF 316

Query: 328 LPECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKRSGHR-SKGRRPNWSGKVKGIVA 387
            PECLLDCLDLSSEH  +E+ANR+E+S+++W    HKR+  + +   + +W   VK ++ 
Sbjct: 317 SPECLLDCLDLSSEHQAIEIANRVESSIYLW----HKRTNSKPATNTKTSWE-MVKELMV 376

Query: 388 DSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQYNKVILSIPLVVFINWSQGCLMP 447
           D+ K   +A RAE+LL SL+ RFPGLPQT LDM+KIQYNK                    
Sbjct: 377 DADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNK-------------------- 436

Query: 448 KTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAETISLFNRGLGGLP 507
                D+G+SILESYSRV+ESLAFNI+ARIDD+L+VDD  +   +    +L N G     
Sbjct: 437 -----DIGKSILESYSRVLESLAFNIVARIDDLLFVDDLTRHSSDQIPTTLGNNG----- 496

Query: 508 IQKRMSPSPFSIHHSPFASPFATPT---FCSSTPIGGSPGR----ASPTVKR 551
                +P   ++  S + +P  +P+     SS  +  SP R     S +VKR
Sbjct: 497 ---NDAPKSIAVPVSNYTTPSYSPSKQELRSSITVPPSPSRFKIPHSSSVKR 497

BLAST of MC02g0334 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 4.5e-122
Identity = 254/539 (47.12%), Postives = 339/539 (62.89%), Query Frame = 0

Query: 21  SLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNFCFPPPVSLPV---------I 80
           S+   ++ES+  SS S   ++    ++  +SSP P        P                
Sbjct: 21  SMEDILTESKERSSTSGASYESSSTTTVASSSPPPPPSQILGWPIRKASFRKNSKESVNF 80

Query: 81  GGKDVFVWDDKS-KKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLS 140
             K + + DD   K ++ + ++VEMMKERFAKLLLGEDMSG GKGVCTALAISNAITNL 
Sbjct: 81  DHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALAISNAITNLC 140

Query: 141 ATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRS 200
           AT+FG+LWRLEPL+ +K+ MW REMEW+L V D IVEL P+ Q +P G  +EVM  +PR 
Sbjct: 141 ATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTCRPRF 200

Query: 201 DLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSIRQEDKW 260
           DL++NLPAL+KLD MLL +L  F  TEFWYVD+GIV  + ND  G     R   RQE+KW
Sbjct: 201 DLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASE-ND--GSASFRRKIQRQEEKW 260

Query: 261 WLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKSGK 320
           WLP P++  +GL+E+AR  L   R+C  QILKAAMAINS  L EM++P +Y ETLPK+G+
Sbjct: 261 WLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNGR 320

Query: 321 ACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKRSG--HR 380
           +CLGD+IYRY+T+D F  E LLDCLDLSSEH  +++ANR+EAS+++WR +   + G  + 
Sbjct: 321 SCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNNN 380

Query: 381 SKGRRPNWSGK-VKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQYNKVI 440
           +    P  + + VK ++A   K   L  R+ETLL  L+ RFP L QT LD++KIQ+NK  
Sbjct: 381 TSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWNK-- 440

Query: 441 LSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKR 500
                                  D+G+SILESYSR +ESLA NI+ARIDD+LYVDD  K+
Sbjct: 441 -----------------------DIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQ 500

Query: 501 CVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRASP 547
             +   +S  +  +  +   K++ P P+ I  S   +P+ T    S+TP G SP   SP
Sbjct: 501 SDDNNLLS--SPAVSSIIAHKKVVPLPYLI--SASGTPYRTS--FSTTP-GFSPSMISP 524

BLAST of MC02g0334 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 7.0e-115
Identity = 259/587 (44.12%), Postives = 343/587 (58.43%), Query Frame = 0

Query: 26  VSESESCSSFSCRRFDGEGASSSMASSPHPVSGNFCFPPPVSLPVIG------------G 85
           V+ES + SS S     G G+SS  +S    V+      PP    ++G            G
Sbjct: 29  VTESTTDSSLSSSS-SGVGSSSGRSS----VAERSVSSPPTKSQILGWPLGQGSWRKSSG 88

Query: 86  K-----DVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITN 145
           K        + D   K+   + SE+E++KER AKLLLGEDMSG G+GVC ALAISNAITN
Sbjct: 89  KMKKKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITN 148

Query: 146 LSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKP 205
           L A + G+ WRLEP+  +K+ MW RE+E LL V D IVELVP+ Q FP G   EVM  +P
Sbjct: 149 LYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRP 208

Query: 206 RSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSIRQED 265
           RSDL+  LPAL+KLD ML+ +LD F  TEFWYVD+GIV  +    N F   G       D
Sbjct: 209 RSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESARSNSFREDG-------D 268

Query: 266 KWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKS 325
           KWWLP P+VP+DGL+E  RK+L   R+ TNQILKA M+INS  LAEME+P +Y E LPK+
Sbjct: 269 KWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKN 328

Query: 326 GKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKR---S 385
           G++CLGD +YR IT+D+F  + LL+ +DLSSE   VE+ANR+EAS+++WR + H R   S
Sbjct: 329 GRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLIS 388

Query: 386 GHRSKGRRPNWSGKVKGIV---ADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQ 445
            +RS   R  W   VK ++    D  K    A RAE+LL  L+ RFPGL QT LD +KIQ
Sbjct: 389 LYRSTSTR--WGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQ 448

Query: 446 YNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVD 505
           YNK                         DVG+SILESYSRV+ESLA++I  RI++VL++D
Sbjct: 449 YNK-------------------------DVGKSILESYSRVLESLAYSIGVRIEEVLFMD 508

Query: 506 DAIKRCVEAETISLFNR----------GLGGLPIQKRMSPSPFSIHHSPFASPFATPTFC 565
           D  K   + +  S  ++          G  G   +K  +PS FS+  S  ++P+ T +F 
Sbjct: 509 DISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTLSFS 568

Query: 566 SSTPI-----------GGSPGRASPTVKRSNVKE-AGQADKERIWSY 568
           +STP            GG  G  +P +   N++E  G   K+ + +Y
Sbjct: 569 ASTPSYSPMPLISPINGGRGGERAPFLSGRNIRERCGFGPKKALANY 576

BLAST of MC02g0334 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 8.3e-108
Identity = 212/401 (52.87%), Postives = 273/401 (68.08%), Query Frame = 0

Query: 92  EVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKRAMW 151
           E+E MKERFAKLLLGEDMSG GKGVCTA+ ISNAITNL ATVFG+  RLEPL  +KRA+W
Sbjct: 104 ELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALW 163

Query: 152 HREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLYMNLPALKKLDAMLLSMLD 211
            REM  LL VCD IVE +P  Q    G T EVM S+PR+D+Y+NLPAL+KLD+ML+  LD
Sbjct: 164 KREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALD 223

Query: 212 GFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSI--RQEDKWWLPCPKVPADGLSEDARKR 271
            F +TEFWY + G +   +          R  I  R+E+KWWLP P VP++GLS+ ARK+
Sbjct: 224 SFQNTEFWYAEEGSL--SMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQ 283

Query: 272 LQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKSGKACLGDIIYRYIT-ADHFLP 331
           L+  R+ TNQI KAAMAINS++L+EMEIP +Y  TLPK GK+ +GD IYRY++ +  F P
Sbjct: 284 LKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFP 343

Query: 332 ECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKRSGHRSKGRRPNWSGKVKGIVAD-- 391
           E LLDCL++SSEH  V++A+R+EAS++ WR K    +   S      W+  VK +++   
Sbjct: 344 EQLLDCLNISSEHEAVQLADRVEASMYTWRRKSCLSNSKNS------WN-MVKDLMSTTE 403

Query: 392 -SGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQYNKVILSIPLVVFINWSQGCLMP 451
            + K++ +A RAETLL  L+ R+P L QT LD+ KIQYNK                    
Sbjct: 404 RTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNK-------------------- 463

Query: 452 KTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIK 487
                DVG+++LESYSRV+E LAFNI+A IDDVLYVD  ++
Sbjct: 464 -----DVGKAVLESYSRVLEGLAFNIVAWIDDVLYVDKTMR 470

BLAST of MC02g0334 vs. NCBI nr
Match: XP_022140655.1 (rop guanine nucleotide exchange factor 1 [Momordica charantia])

HSP 1 Score: 1119 bits (2895), Expect = 0.0
Identity = 558/584 (95.55%), Postives = 558/584 (95.55%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF
Sbjct: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG
Sbjct: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300

Query: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360
           YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK
Sbjct: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360

Query: 361 DHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420
           DHKRSGHRSKGRRP WSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK
Sbjct: 361 DHKRSGHRSKGRRPTWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420

Query: 421 IQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLY 480
           IQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVLY
Sbjct: 421 IQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVLY 480

Query: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540
           VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS
Sbjct: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540

Query: 541 PGRASPTVKRSNVKEAGQADKERIWSYAGNLSGRRISGVTPERD 584
           PGRASPTVKRSNVKEAGQADKERIWSYAGNLSGRRISGVTPERD
Sbjct: 541 PGRASPTVKRSNVKEAGQADKERIWSYAGNLSGRRISGVTPERD 559

BLAST of MC02g0334 vs. NCBI nr
Match: XP_038901121.1 (rop guanine nucleotide exchange factor 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1048 bits (2709), Expect = 0.0
Identity = 525/594 (88.38%), Postives = 544/594 (91.58%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF
Sbjct: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLPV+ GKDVF+WDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPVLAGKDVFLWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSK RSDL+MNLPALKKLDA+LL +L GFCHTEFWYVDRGIV+GDLNDCN FLPGG
Sbjct: 181 YEVMMSKLRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEME+PTA
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPTA 300

Query: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360
           Y ETLPKSGKACLGDIIYRY+TADHF PECLLDCLDLSSEHHT+E+ANRIEAS+HIWR K
Sbjct: 301 YMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQK 360

Query: 361 DHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420
           DHKRSGHRSKGRRP WSGKVKGIVAD  KS+NLARRAETLL+SLRLRFPGLPQT LDMAK
Sbjct: 361 DHKRSGHRSKGRRPTWSGKVKGIVADPRKSNNLARRAETLLDSLRLRFPGLPQTALDMAK 420

Query: 421 IQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLY 480
           IQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVLY
Sbjct: 421 IQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVLY 480

Query: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540
           VDDAIKRCVEAE+ISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS
Sbjct: 481 VDDAIKRCVEAESISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540

Query: 541 PGRASP--TVKRSN-VKEA-------GQADKERIWSYAGNLSGRRISGVTPERD 584
           PGR SP  TVKRSN +KE         Q++KERIWSYAGNLSGRR+SGVTPERD
Sbjct: 541 PGRLSPSPTVKRSNNMKETVLRQDKQSQSEKERIWSYAGNLSGRRVSGVTPERD 569

BLAST of MC02g0334 vs. NCBI nr
Match: XP_038901120.1 (rop guanine nucleotide exchange factor 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1043 bits (2697), Expect = 0.0
Identity = 525/595 (88.24%), Postives = 544/595 (91.43%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF
Sbjct: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLPV+ GKDVF+WDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPVLAGKDVFLWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSK RSDL+MNLPALKKLDA+LL +L GFCHTEFWYVDRGIV+GDLNDCN FLPGG
Sbjct: 181 YEVMMSKLRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEME+PTA
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPTA 300

Query: 301 YKETLPK-SGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRA 360
           Y ETLPK SGKACLGDIIYRY+TADHF PECLLDCLDLSSEHHT+E+ANRIEAS+HIWR 
Sbjct: 301 YMETLPKQSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQ 360

Query: 361 KDHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMA 420
           KDHKRSGHRSKGRRP WSGKVKGIVAD  KS+NLARRAETLL+SLRLRFPGLPQT LDMA
Sbjct: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPRKSNNLARRAETLLDSLRLRFPGLPQTALDMA 420

Query: 421 KIQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVL 480
           KIQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVL
Sbjct: 421 KIQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVL 480

Query: 481 YVDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGG 540
           YVDDAIKRCVEAE+ISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGG
Sbjct: 481 YVDDAIKRCVEAESISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGG 540

Query: 541 SPGRASP--TVKRSN-VKEA-------GQADKERIWSYAGNLSGRRISGVTPERD 584
           SPGR SP  TVKRSN +KE         Q++KERIWSYAGNLSGRR+SGVTPERD
Sbjct: 541 SPGRLSPSPTVKRSNNMKETVLRQDKQSQSEKERIWSYAGNLSGRRVSGVTPERD 570

BLAST of MC02g0334 vs. NCBI nr
Match: XP_004139953.1 (rop guanine nucleotide exchange factor 1 [Cucumis sativus] >KGN46713.1 hypothetical protein Csa_020632 [Cucumis sativus])

HSP 1 Score: 1040 bits (2688), Expect = 0.0
Identity = 516/595 (86.72%), Postives = 541/595 (90.92%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           MGSVSSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVS NF
Sbjct: 1   MGSVSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSANF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLPV+GGKDVFVWDDKSKKR+ADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPVLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSKPRSDL+MNLPALKKLDA+LL +L GFCHTEFWYVDRGIV+GDLNDCN FLPGG
Sbjct: 181 YEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEME+P A
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPAA 300

Query: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360
           Y ETLPKSGKACLGDIIYRY+TADHF PECLLDCLDLSSEHHT+E+ANRIEAS+HIWR K
Sbjct: 301 YMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQK 360

Query: 361 DHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420
           DHKRSGHR+KGRRP WSGKVKGIV D  KS+NLARRAETLL+SLRLRFPGLPQT LDMAK
Sbjct: 361 DHKRSGHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMAK 420

Query: 421 IQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLY 480
           IQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVLY
Sbjct: 421 IQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVLY 480

Query: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540
           VDDAIKRCV+AE+ISLFNRGLGGLPIQKRMSPSPFSIH SPFASPFATPTFCSSTP+GGS
Sbjct: 481 VDDAIKRCVKAESISLFNRGLGGLPIQKRMSPSPFSIHQSPFASPFATPTFCSSTPVGGS 540

Query: 541 PGRASP--TVKRSNVKEA---------GQADKERIWSYAGNLSGRRISGVTPERD 584
           PGR SP  TVKRSN K+           Q++K++IWSYAGNLSGRR+SGVTPERD
Sbjct: 541 PGRLSPPPTVKRSNTKKETAVLSKDKQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of MC02g0334 vs. NCBI nr
Match: XP_008448331.1 (PREDICTED: rop guanine nucleotide exchange factor 1 [Cucumis melo] >KAA0061984.1 rop guanine nucleotide exchange factor 1 [Cucumis melo var. makuwa] >TYK30532.1 rop guanine nucleotide exchange factor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1033 bits (2671), Expect = 0.0
Identity = 513/595 (86.22%), Postives = 538/595 (90.42%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           M S+SSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVS NF
Sbjct: 1   MSSMSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSANF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLP++GGKDVFVWDDKSKKR+ADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPLLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSKPRSDL+MNLPALKKLDA+LL +L GFCHTEFWYVDRGIV+GDLNDCN FLPGG
Sbjct: 181 YEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVL EME+P A
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLVEMEVPAA 300

Query: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360
           Y ETLPKSGKACLGDIIYRY+TADHF PECLLDCLDLSSEHHT+E+ANRIEAS+HIWR K
Sbjct: 301 YMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQK 360

Query: 361 DHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420
           DHKRS HR+KGRRP WSGKVKGIV D  KS+NLARRAETLL+SLRLRFPGLPQT LDMAK
Sbjct: 361 DHKRSAHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMAK 420

Query: 421 IQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLY 480
           IQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVLY
Sbjct: 421 IQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVLY 480

Query: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540
           VDDAIKRCVEAE+ISLFNRGLGGLPIQKRMSPSPFSIH SPFASPFATPTFCSSTPIGGS
Sbjct: 481 VDDAIKRCVEAESISLFNRGLGGLPIQKRMSPSPFSIHQSPFASPFATPTFCSSTPIGGS 540

Query: 541 PGRASP--TVKRSNVKEA---------GQADKERIWSYAGNLSGRRISGVTPERD 584
           PGR SP  TVKRSN K+           Q++K++IWSYAGNLSGRR+SGVTPERD
Sbjct: 541 PGRLSPSPTVKRSNTKKETAVLSKDKQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of MC02g0334 vs. ExPASy TrEMBL
Match: A0A6J1CFP8 (rop guanine nucleotide exchange factor 1 OS=Momordica charantia OX=3673 GN=LOC111011261 PE=4 SV=1)

HSP 1 Score: 1119 bits (2895), Expect = 0.0
Identity = 558/584 (95.55%), Postives = 558/584 (95.55%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF
Sbjct: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG
Sbjct: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300

Query: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360
           YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK
Sbjct: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360

Query: 361 DHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420
           DHKRSGHRSKGRRP WSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK
Sbjct: 361 DHKRSGHRSKGRRPTWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420

Query: 421 IQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLY 480
           IQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVLY
Sbjct: 421 IQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVLY 480

Query: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540
           VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS
Sbjct: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540

Query: 541 PGRASPTVKRSNVKEAGQADKERIWSYAGNLSGRRISGVTPERD 584
           PGRASPTVKRSNVKEAGQADKERIWSYAGNLSGRRISGVTPERD
Sbjct: 541 PGRASPTVKRSNVKEAGQADKERIWSYAGNLSGRRISGVTPERD 559

BLAST of MC02g0334 vs. ExPASy TrEMBL
Match: A0A0A0KB11 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G126260 PE=4 SV=1)

HSP 1 Score: 1040 bits (2688), Expect = 0.0
Identity = 516/595 (86.72%), Postives = 541/595 (90.92%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           MGSVSSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVS NF
Sbjct: 1   MGSVSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSANF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLPV+GGKDVFVWDDKSKKR+ADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPVLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSKPRSDL+MNLPALKKLDA+LL +L GFCHTEFWYVDRGIV+GDLNDCN FLPGG
Sbjct: 181 YEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEME+P A
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPAA 300

Query: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360
           Y ETLPKSGKACLGDIIYRY+TADHF PECLLDCLDLSSEHHT+E+ANRIEAS+HIWR K
Sbjct: 301 YMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQK 360

Query: 361 DHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420
           DHKRSGHR+KGRRP WSGKVKGIV D  KS+NLARRAETLL+SLRLRFPGLPQT LDMAK
Sbjct: 361 DHKRSGHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMAK 420

Query: 421 IQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLY 480
           IQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVLY
Sbjct: 421 IQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVLY 480

Query: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540
           VDDAIKRCV+AE+ISLFNRGLGGLPIQKRMSPSPFSIH SPFASPFATPTFCSSTP+GGS
Sbjct: 481 VDDAIKRCVKAESISLFNRGLGGLPIQKRMSPSPFSIHQSPFASPFATPTFCSSTPVGGS 540

Query: 541 PGRASP--TVKRSNVKEA---------GQADKERIWSYAGNLSGRRISGVTPERD 584
           PGR SP  TVKRSN K+           Q++K++IWSYAGNLSGRR+SGVTPERD
Sbjct: 541 PGRLSPPPTVKRSNTKKETAVLSKDKQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of MC02g0334 vs. ExPASy TrEMBL
Match: A0A5D3E3R0 (Rop guanine nucleotide exchange factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1985G00080 PE=4 SV=1)

HSP 1 Score: 1033 bits (2671), Expect = 0.0
Identity = 513/595 (86.22%), Postives = 538/595 (90.42%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           M S+SSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVS NF
Sbjct: 1   MSSMSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSANF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLP++GGKDVFVWDDKSKKR+ADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPLLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSKPRSDL+MNLPALKKLDA+LL +L GFCHTEFWYVDRGIV+GDLNDCN FLPGG
Sbjct: 181 YEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVL EME+P A
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLVEMEVPAA 300

Query: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360
           Y ETLPKSGKACLGDIIYRY+TADHF PECLLDCLDLSSEHHT+E+ANRIEAS+HIWR K
Sbjct: 301 YMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQK 360

Query: 361 DHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420
           DHKRS HR+KGRRP WSGKVKGIV D  KS+NLARRAETLL+SLRLRFPGLPQT LDMAK
Sbjct: 361 DHKRSAHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMAK 420

Query: 421 IQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLY 480
           IQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVLY
Sbjct: 421 IQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVLY 480

Query: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540
           VDDAIKRCVEAE+ISLFNRGLGGLPIQKRMSPSPFSIH SPFASPFATPTFCSSTPIGGS
Sbjct: 481 VDDAIKRCVEAESISLFNRGLGGLPIQKRMSPSPFSIHQSPFASPFATPTFCSSTPIGGS 540

Query: 541 PGRASP--TVKRSNVKEA---------GQADKERIWSYAGNLSGRRISGVTPERD 584
           PGR SP  TVKRSN K+           Q++K++IWSYAGNLSGRR+SGVTPERD
Sbjct: 541 PGRLSPSPTVKRSNTKKETAVLSKDKQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of MC02g0334 vs. ExPASy TrEMBL
Match: A0A1S3BKB9 (rop guanine nucleotide exchange factor 1 OS=Cucumis melo OX=3656 GN=LOC103490557 PE=4 SV=1)

HSP 1 Score: 1033 bits (2671), Expect = 0.0
Identity = 513/595 (86.22%), Postives = 538/595 (90.42%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           M S+SSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVS NF
Sbjct: 1   MSSMSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSANF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLP++GGKDVFVWDDKSKKR+ADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPLLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGG 240
           YEVMMSKPRSDL+MNLPALKKLDA+LL +L GFCHTEFWYVDRGIV+GDLNDCN FLPGG
Sbjct: 181 YEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPGG 240

Query: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTA 300
           RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVL EME+P A
Sbjct: 241 RPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLVEMEVPAA 300

Query: 301 YKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAK 360
           Y ETLPKSGKACLGDIIYRY+TADHF PECLLDCLDLSSEHHT+E+ANRIEAS+HIWR K
Sbjct: 301 YMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQK 360

Query: 361 DHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAK 420
           DHKRS HR+KGRRP WSGKVKGIV D  KS+NLARRAETLL+SLRLRFPGLPQT LDMAK
Sbjct: 361 DHKRSAHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMAK 420

Query: 421 IQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLY 480
           IQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVLY
Sbjct: 421 IQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVLY 480

Query: 481 VDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGS 540
           VDDAIKRCVEAE+ISLFNRGLGGLPIQKRMSPSPFSIH SPFASPFATPTFCSSTPIGGS
Sbjct: 481 VDDAIKRCVEAESISLFNRGLGGLPIQKRMSPSPFSIHQSPFASPFATPTFCSSTPIGGS 540

Query: 541 PGRASP--TVKRSNVKEA---------GQADKERIWSYAGNLSGRRISGVTPERD 584
           PGR SP  TVKRSN K+           Q++K++IWSYAGNLSGRR+SGVTPERD
Sbjct: 541 PGRLSPSPTVKRSNTKKETAVLSKDKQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of MC02g0334 vs. ExPASy TrEMBL
Match: A0A6J1G702 (rop guanine nucleotide exchange factor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451330 PE=4 SV=1)

HSP 1 Score: 1032 bits (2669), Expect = 0.0
Identity = 521/597 (87.27%), Postives = 535/597 (89.61%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60
           M SVSSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF
Sbjct: 1   MASVSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNF 60

Query: 61  CFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120
           CFPPPVSLPVIGGKDV VWD K KKRD DLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL
Sbjct: 61  CFPPPVSLPVIGGKDVLVWDGKPKKRDDDLSEVEMMKERFAKLLLGEDMSGGGKGVCTAL 120

Query: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180
           AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT
Sbjct: 121 AISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGT 180

Query: 181 YEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNG-FLPG 240
           YEVMMSKPRSDL+MNLPALKKLDAMLL+MLDGFCHTEFWYVDRGIV+GDLNDCN  FLPG
Sbjct: 181 YEVMMSKPRSDLHMNLPALKKLDAMLLAMLDGFCHTEFWYVDRGIVLGDLNDCNNDFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEME+PT
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300

Query: 301 AYKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRA 360
           AY ETLP+SGKACLGDI+YRYITADHF PECLLDCLDLSSEHHT+E+ANRIEASVHIWR 
Sbjct: 301 AYMETLPRSGKACLGDIVYRYITADHFSPECLLDCLDLSSEHHTLEIANRIEASVHIWRL 360

Query: 361 KDHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMA 420
           KD KRSGHRS GRRP WSGKVKG+VA  GKSHN+ARRAETLLESLRLRFPGLPQT LDMA
Sbjct: 361 KDQKRSGHRSTGRRPTWSGKVKGMVAVPGKSHNMARRAETLLESLRLRFPGLPQTALDMA 420

Query: 421 KIQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVL 480
           KIQYNK                         DVGQSILESYSRVMESLAFNIMARIDDVL
Sbjct: 421 KIQYNK-------------------------DVGQSILESYSRVMESLAFNIMARIDDVL 480

Query: 481 YVDDAIKRCVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGG 540
           YVDDAIK+CVEAE+ISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPF TPTFCSSTPI G
Sbjct: 481 YVDDAIKQCVEAESISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFGTPTFCSSTPIEG 540

Query: 541 SPGRASP--TVKRSNVKEAGQ----------ADKERIWSYAGNLSGRRISGVTPERD 584
           SP R SP  TVKRSNVKE             A  E+IWSYAGNLSGRRISGVTPERD
Sbjct: 541 SPERLSPSPTVKRSNVKETPDVLRPDKHSQAAGNEKIWSYAGNLSGRRISGVTPERD 572

BLAST of MC02g0334 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 734.6 bits (1895), Expect = 6.3e-212
Identity = 391/589 (66.38%), Postives = 455/589 (77.25%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSG-N 60
           MGS+SS++  +  SERCGSYS SAD+SESES SSFSC RFDGEGASSS+ SSP  V+G  
Sbjct: 1   MGSLSSEEDDEVSSERCGSYSPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVAGRG 60

Query: 61  FCFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           F FP PV LPVIGGKDV VWDD  K+ D DLSE+EMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FYFPAPVMLPVIGGKDV-VWDD--KQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSATVFGELWRLEPLAPQK+AMW RE+EWLLCV DSIVEL+P++Q FPGGG
Sbjct: 121 LAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGG 180

Query: 181 TYEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPG 240
           TYE+M ++PRSDLY NLPALKKLDAML+ MLD F  TEFWY DRGIV+G   DC+     
Sbjct: 181 TYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLG---DCDKDSYN 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPT 300
              S+RQEDKWWLPCPKVP +GLSE+ARK+LQQCRD  NQILKAA+AINS VLAEMEIP 
Sbjct: 241 SPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPD 300

Query: 301 AYKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRA 360
            Y ETLPKSGK CLG+IIY+Y+TA+ F PECLLDCLDLSSEH T+E+ANRIEA+VH+WR 
Sbjct: 301 PYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQ 360

Query: 361 KDHKRSGHRSKGRRPNWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMA 420
           K+ +R   ++K +  +W GKVKG+V D+ ++  L +RAETLL+SLR+RFPGLPQT LDM 
Sbjct: 361 KNGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMN 420

Query: 421 KIQYNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVL 480
           KIQYNK                         DVGQSILESYSRVMES+AFNI ARIDDVL
Sbjct: 421 KIQYNK-------------------------DVGQSILESYSRVMESMAFNITARIDDVL 480

Query: 481 YVDDAIKRCVE-AETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIG 540
           YVDDA++R +   E++SLF+  + GL  QK      FS+  SP  SPFATP    ++   
Sbjct: 481 YVDDAMRRSISVTESLSLFS--INGLNPQK-----AFSVQSSPHGSPFATPALSVAS--- 540

Query: 541 GSPGRASP--TVKRSNVKEAG-QADKERIWSYAGNLSGRRISGVTPERD 585
            SP RA P  +VKR+  +E G   + E+ WSYAGNLS RR++GVTPERD
Sbjct: 541 RSPRRAPPLYSVKRNGTREKGIVGETEKAWSYAGNLSSRRVTGVTPERD 548

BLAST of MC02g0334 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 475.7 bits (1223), Expect = 5.3e-134
Identity = 270/532 (50.75%), Postives = 348/532 (65.41%), Query Frame = 0

Query: 28  ESESCSSFSCRRFDGEGASSSMAS-SPHPVSGNFCFPPPVSLPVIGGKDVFVWDDKSKKR 87
           ES  CSS + R+ + E + S     +  PVS  +      S+  I G      + K  + 
Sbjct: 17  ESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRW------SVKNIDG------EKKKIRS 76

Query: 88  DADLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQ 147
           D+ +SEVEMMKERF+KLLLGEDMSG G GVCTALAISNAITNL AT+FG+LWRLEPL  +
Sbjct: 77  DSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTE 136

Query: 148 KRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLYMNLPALKKLDAML 207
           K+ MW REMEWLLCV D IVE+ PT Q FP G   E+M  +PRSDLY+NLPAL+KLD ML
Sbjct: 137 KKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNML 196

Query: 208 LSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSIRQEDKWWLPCPKVPADGLSEDA 267
           L +LD F  TEFWYVD+GI+  + +  +G     +   RQEDKWWLP P+V   GL E++
Sbjct: 197 LEILDSFEETEFWYVDQGIMAHE-SAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQENS 256

Query: 268 RKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKSGKACLGDIIYRYITADHF 327
           RK+LQ  RDCTNQILKAAMAINS  LA+MEIP +Y E+LP+ G++CLGD+IYRYI++D F
Sbjct: 257 RKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQF 316

Query: 328 LPECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKRSGHR-SKGRRPNWSGKVKGIVA 387
            PECLLDCLDLSSEH  +E+ANR+E+S+++W    HKR+  + +   + +W   VK ++ 
Sbjct: 317 SPECLLDCLDLSSEHQAIEIANRVESSIYLW----HKRTNSKPATNTKTSWE-MVKELMV 376

Query: 388 DSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQYNKVILSIPLVVFINWSQGCLMP 447
           D+ K   +A RAE+LL SL+ RFPGLPQT LDM+KIQYNK                    
Sbjct: 377 DADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNK-------------------- 436

Query: 448 KTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAETISLFNRGLGGLP 507
                D+G+SILESYSRV+ESLAFNI+ARIDD+L+VDD  +   +    +L N G     
Sbjct: 437 -----DIGKSILESYSRVLESLAFNIVARIDDLLFVDDLTRHSSDQIPTTLGNNG----- 496

Query: 508 IQKRMSPSPFSIHHSPFASPFATPT---FCSSTPIGGSPGR----ASPTVKR 551
                +P   ++  S + +P  +P+     SS  +  SP R     S +VKR
Sbjct: 497 ---NDAPKSIAVPVSNYTTPSYSPSKQELRSSITVPPSPSRFKIPHSSSVKR 497

BLAST of MC02g0334 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 439.9 bits (1130), Expect = 3.2e-123
Identity = 254/539 (47.12%), Postives = 339/539 (62.89%), Query Frame = 0

Query: 21  SLSADVSESESCSSFSCRRFDGEGASSSMASSPHPVSGNFCFPPPVSLPV---------I 80
           S+   ++ES+  SS S   ++    ++  +SSP P        P                
Sbjct: 21  SMEDILTESKERSSTSGASYESSSTTTVASSSPPPPPSQILGWPIRKASFRKNSKESVNF 80

Query: 81  GGKDVFVWDDKS-KKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLS 140
             K + + DD   K ++ + ++VEMMKERFAKLLLGEDMSG GKGVCTALAISNAITNL 
Sbjct: 81  DHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALAISNAITNLC 140

Query: 141 ATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRS 200
           AT+FG+LWRLEPL+ +K+ MW REMEW+L V D IVEL P+ Q +P G  +EVM  +PR 
Sbjct: 141 ATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTCRPRF 200

Query: 201 DLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSIRQEDKW 260
           DL++NLPAL+KLD MLL +L  F  TEFWYVD+GIV  + ND  G     R   RQE+KW
Sbjct: 201 DLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASE-ND--GSASFRRKIQRQEEKW 260

Query: 261 WLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKSGK 320
           WLP P++  +GL+E+AR  L   R+C  QILKAAMAINS  L EM++P +Y ETLPK+G+
Sbjct: 261 WLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNGR 320

Query: 321 ACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKRSG--HR 380
           +CLGD+IYRY+T+D F  E LLDCLDLSSEH  +++ANR+EAS+++WR +   + G  + 
Sbjct: 321 SCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNNN 380

Query: 381 SKGRRPNWSGK-VKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQYNKVI 440
           +    P  + + VK ++A   K   L  R+ETLL  L+ RFP L QT LD++KIQ+NK  
Sbjct: 381 TSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWNK-- 440

Query: 441 LSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKR 500
                                  D+G+SILESYSR +ESLA NI+ARIDD+LYVDD  K+
Sbjct: 441 -----------------------DIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQ 500

Query: 501 CVEAETISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRASP 547
             +   +S  +  +  +   K++ P P+ I  S   +P+ T    S+TP G SP   SP
Sbjct: 501 SDDNNLLS--SPAVSSIIAHKKVVPLPYLI--SASGTPYRTS--FSTTP-GFSPSMISP 524

BLAST of MC02g0334 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 416.0 bits (1068), Expect = 5.0e-116
Identity = 259/587 (44.12%), Postives = 343/587 (58.43%), Query Frame = 0

Query: 26  VSESESCSSFSCRRFDGEGASSSMASSPHPVSGNFCFPPPVSLPVIG------------G 85
           V+ES + SS S     G G+SS  +S    V+      PP    ++G            G
Sbjct: 29  VTESTTDSSLSSSS-SGVGSSSGRSS----VAERSVSSPPTKSQILGWPLGQGSWRKSSG 88

Query: 86  K-----DVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITN 145
           K        + D   K+   + SE+E++KER AKLLLGEDMSG G+GVC ALAISNAITN
Sbjct: 89  KMKKKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITN 148

Query: 146 LSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKP 205
           L A + G+ WRLEP+  +K+ MW RE+E LL V D IVELVP+ Q FP G   EVM  +P
Sbjct: 149 LYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRP 208

Query: 206 RSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSIRQED 265
           RSDL+  LPAL+KLD ML+ +LD F  TEFWYVD+GIV  +    N F   G       D
Sbjct: 209 RSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESARSNSFREDG-------D 268

Query: 266 KWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKS 325
           KWWLP P+VP+DGL+E  RK+L   R+ TNQILKA M+INS  LAEME+P +Y E LPK+
Sbjct: 269 KWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKN 328

Query: 326 GKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKR---S 385
           G++CLGD +YR IT+D+F  + LL+ +DLSSE   VE+ANR+EAS+++WR + H R   S
Sbjct: 329 GRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLIS 388

Query: 386 GHRSKGRRPNWSGKVKGIV---ADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQ 445
            +RS   R  W   VK ++    D  K    A RAE+LL  L+ RFPGL QT LD +KIQ
Sbjct: 389 LYRSTSTR--WGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQ 448

Query: 446 YNKVILSIPLVVFINWSQGCLMPKTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVD 505
           YNK                         DVG+SILESYSRV+ESLA++I  RI++VL++D
Sbjct: 449 YNK-------------------------DVGKSILESYSRVLESLAYSIGVRIEEVLFMD 508

Query: 506 DAIKRCVEAETISLFNR----------GLGGLPIQKRMSPSPFSIHHSPFASPFATPTFC 565
           D  K   + +  S  ++          G  G   +K  +PS FS+  S  ++P+ T +F 
Sbjct: 509 DISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTLSFS 568

Query: 566 SSTPI-----------GGSPGRASPTVKRSNVKE-AGQADKERIWSY 568
           +STP            GG  G  +P +   N++E  G   K+ + +Y
Sbjct: 569 ASTPSYSPMPLISPINGGRGGERAPFLSGRNIRERCGFGPKKALANY 576

BLAST of MC02g0334 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 392.5 bits (1007), Expect = 5.9e-109
Identity = 212/401 (52.87%), Postives = 273/401 (68.08%), Query Frame = 0

Query: 92  EVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKRAMW 151
           E+E MKERFAKLLLGEDMSG GKGVCTA+ ISNAITNL ATVFG+  RLEPL  +KRA+W
Sbjct: 104 ELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALW 163

Query: 152 HREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLYMNLPALKKLDAMLLSMLD 211
            REM  LL VCD IVE +P  Q    G T EVM S+PR+D+Y+NLPAL+KLD+ML+  LD
Sbjct: 164 KREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALD 223

Query: 212 GFCHTEFWYVDRGIVVGDLNDCNGFLPGGRPSI--RQEDKWWLPCPKVPADGLSEDARKR 271
            F +TEFWY + G +   +          R  I  R+E+KWWLP P VP++GLS+ ARK+
Sbjct: 224 SFQNTEFWYAEEGSL--SMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQ 283

Query: 272 LQQCRDCTNQILKAAMAINSNVLAEMEIPTAYKETLPKSGKACLGDIIYRYIT-ADHFLP 331
           L+  R+ TNQI KAAMAINS++L+EMEIP +Y  TLPK GK+ +GD IYRY++ +  F P
Sbjct: 284 LKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFP 343

Query: 332 ECLLDCLDLSSEHHTVEVANRIEASVHIWRAKDHKRSGHRSKGRRPNWSGKVKGIVAD-- 391
           E LLDCL++SSEH  V++A+R+EAS++ WR K    +   S      W+  VK +++   
Sbjct: 344 EQLLDCLNISSEHEAVQLADRVEASMYTWRRKSCLSNSKNS------WN-MVKDLMSTTE 403

Query: 392 -SGKSHNLARRAETLLESLRLRFPGLPQTGLDMAKIQYNKVILSIPLVVFINWSQGCLMP 451
            + K++ +A RAETLL  L+ R+P L QT LD+ KIQYNK                    
Sbjct: 404 RTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNK-------------------- 463

Query: 452 KTFLQDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIK 487
                DVG+++LESYSRV+E LAFNI+A IDDVLYVD  ++
Sbjct: 464 -----DVGKAVLESYSRVLEGLAFNIVAWIDDVLYVDKTMR 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93ZY28.9e-21166.38Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9LZN07.5e-13350.75Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
F4K2954.5e-12247.12Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9M0567.0e-11544.12Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
A4IJ278.3e-10852.87Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Match NameE-valueIdentityDescription
XP_022140655.10.095.55rop guanine nucleotide exchange factor 1 [Momordica charantia][more]
XP_038901121.10.088.38rop guanine nucleotide exchange factor 1 isoform X2 [Benincasa hispida][more]
XP_038901120.10.088.24rop guanine nucleotide exchange factor 1 isoform X1 [Benincasa hispida][more]
XP_004139953.10.086.72rop guanine nucleotide exchange factor 1 [Cucumis sativus] >KGN46713.1 hypotheti... [more]
XP_008448331.10.086.22PREDICTED: rop guanine nucleotide exchange factor 1 [Cucumis melo] >KAA0061984.1... [more]
Match NameE-valueIdentityDescription
A0A6J1CFP80.095.55rop guanine nucleotide exchange factor 1 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A0A0KB110.086.72PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G126260 PE=4 ... [more]
A0A5D3E3R00.086.22Rop guanine nucleotide exchange factor 1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3BKB90.086.22rop guanine nucleotide exchange factor 1 OS=Cucumis melo OX=3656 GN=LOC103490557... [more]
A0A6J1G7020.087.27rop guanine nucleotide exchange factor 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT4G38430.16.3e-21266.38rho guanyl-nucleotide exchange factor 1 [more]
AT5G02010.15.3e-13450.75RHO guanyl-nucleotide exchange factor 7 [more]
AT5G05940.13.2e-12347.12ROP guanine nucleotide exchange factor 5 [more]
AT3G55660.15.0e-11644.12ROP (rho of plants) guanine nucleotide exchange factor 6 [more]
AT4G00460.25.9e-10952.87RHO guanyl-nucleotide exchange factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.58.2010PRONE domain, subdomain 1coord: 450..483
e-value: 3.0E-11
score: 45.1
NoneNo IPR availableGENE3D1.20.58.2010PRONE domain, subdomain 1coord: 83..231
e-value: 1.5E-69
score: 235.4
NoneNo IPR availableGENE3D1.20.58.1310PRONE domain, subdomain 2coord: 296..423
e-value: 1.6E-44
score: 152.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 535..557
NoneNo IPR availablePANTHERPTHR33101:SF6ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 1..425
NoneNo IPR availablePANTHERPTHR33101:SF6ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 448..584
IPR005512PRONE domainPFAMPF03759PRONEcoord: 449..486
e-value: 8.6E-15
score: 54.4
coord: 91..427
e-value: 1.3E-154
score: 514.4
IPR005512PRONE domainPROSITEPS51334PRONEcoord: 83..492
score: 110.791611
IPR038937Rop guanine nucleotide exchange factorPANTHERPTHR33101ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 1..425
coord: 448..584

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0334.1MC02g0334.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity