MC02g0197 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0197
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPoly [ADP-ribose] polymerase
LocationMC02: 1850743 .. 1854948 (-)
RNA-Seq ExpressionMC02g0197
SyntenyMC02g0197
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAAAATTTTGAAGGTTCATGAAACGAGGTCCCATTCTCACACTCATGGTTCTGGTGAAGAAGAAAAGAAAGTGACAAGGAAGCAGAAGGCAGAGAGCAAGACACATGAAAGCGAACTTTCGCCAAAGAAAGTCAAGTCCGAAGATGAAAATAGCCGCCCCAACGGCAAATCCGAAGTCAGCCTTGCAGATGAATTTGAAGAGTTCTGTAAGGCTACGAGAGAGAGTCTCTCCATTGATCAAATGAAGGAGATTCTGCAAATGAATGACCAAGACTCCACTGGCCCTGACTATGAAGTTGCTTCTAAATGGTATACCACCGACAGACAATGCACATTGTAGCTTTTTCCTTATACCATTTGAACCGTTATTTTTCTTTAGCAATGCTAAAGAAAAATTCATAGATATTGTTGTATGTTTCTGAGACTGTTCTTACGTGGACAGTCAAGACTTGCTGTTCTATGGTGTCCTAGAAACATGCCCAGTTTGTAGTGGCAAGCTGGTATTTGATGGGAAGCGCTACTCTTGCAATGGCTTTCTAAGTGAATGGTCTACCTGTACCTTCAAAACGAAGGATCCTCCTAGGAAAAATGAAACCAGCAAGTTACCAGAATCAGTCTTGAATTCTCCCATTTCAGATGTAACCCTCCATTTCCCTACTTGTTTCTTCTTTTCCTAGATAGCCAACATTGTCATAGATCTCTGTTTCTTAATGGTGTAGTTGATAAAGAAGCATCAGAATCCGGGTCAACGTCCAAAGAGAAATCTTGGCCAGACAGATAAACCCTTCTCTGGAATGACAATTTCACTCTCTGGTCGTCTATCGAGAACTCATGTAAGTTCATGGGTTGAGATTGAGCTTTTATGGCACAAGTTTTTAACTTCCTACTACTATGTTTAACAAGTTTCCAACGATTGTCAACTCAGCATTACTGGAGAAAAGAAATTGAGAAGCATGGAGGGACCGTTAACAATTCTGTAATTGGTACGTTTTCTTTATCCTCGCCAAGTCCTTTTGTGTTGTCTTCGCTGTAATCACTGGAAGTATTGATGAACAAATCTTTGCAGGTGTCAGTTGCTTGGTTGTCTCTCCTGCTGAGAGAGAGCGTGGTGGATCTTCTAAAGTTGCAGAAGCAGTGTATATTTCTTCCTAACTAGCCAAGATATATGTAGTTTAAGGGCTGTTTGGTTTTCAAATTTTCATACTTGGGTTTAGGCTTTTACTCTCTCTCTCTCCATGTACTGTTGTTAAAACTTGAAACCAAGGCGCACCAAACAAGAAGATAAGAAAATTAAAATTGAGAAATGTGGTTTTAAAACCGGGCAAAGTGCTTCATCAAAGAAACGATCTAGTTTTCTGCCTAGTGAATCAAAATCTAGTTAACTGAACAAAATGAATGTTTCTCAAAGGTCCTGCTCTCTTAATAAGTATTCTCTTTTTCAGCGAAACAGGTATACGAGTCATCCGTGAAGCTTGGCTACTTGACAGCATTGAGAAGCAAGAAGCTCAGCCATTGGAAGCTTATGATGTTTTGACAGATCTCGCTGTGGAAGGCAAAGGGATCCCTTGGGATAAACAAGATCCCTCGGAAGAGGCACTTGAATCCCTCACAGCAGAAGTATGCATATAATTTTGAACAAACCGTAGATAAATATATAGAGTTCTTACCAATTACTTTTACATATGTTGTGCAGCTCAAGTTATATGGAAAGAGAGGGGTTTACAAGGATACCAAATTACGAGAACAGGGTGGAGAGATATTTGAAAAGGATGGAATCTTGTATAATTGTGCCTTCTCACTCTGCGATCAAGGCCGGGGACTCAATGAGTAGCATACTGAAACTTTTCTCCAGAAAGTTATTTGATCGTTAATTTTCTCCAGTTGGTTCTTATCCACAAGCTACTGTTGTAGGTATTGTATTGCTCAACTGATCAAAGTACCAGATAAGAACTTGCATCTATACTATAAGAAAGGAAACGTGGGGGATGATCCCAATGCAGAGGAAAGGCTGGAGGAATGGGAAAATGAGAAAACTGCTATAAAGGAGTTTGTGGGGCTGTTCGAAGAAATAACTGGAAATGAATTCGAGCCATGGGAAAGAGAGAAGAAGTTTGAGAAGAAACGCTTGTCGTTTTATCCCATCGATATGGTACAGTTCCAGTGTTTCAGTCAGTTTGTTCATGATATTTGAACTTCTTTATTTATAAGTTATTCTGGGGCAATGGCTATAGGATGATGGAGTAGATGTGAGGCATGGAGGGCTTGGTCTTAGGCAGCTAGGAGTTGCATCTGTTCACAGTAAGCTTGAGCCCGCGATTGCAAACTTTATGAAGGTTTTGTGCAGCCAGGAGATTTACAAGTAAGGAATTGTCTATAGAACCAGATTTCATTTCCCCTTCAATCCCACTCTCGTCATTAGATCTTACATCTTAGTCCTCGGATTTCAGGTATGCTCTCATGGAGATGGCACATGATCCTCCGGAATTACCTATGGGAATGCTTTCTAATCTTCACTTGAAGAGATGTAAGCCCTTCTTGGCCTATTCTCTTTTCTTCTCCTGTTTTTGTTCTTCTGCTCAAGTGTAAAGAGAGAACTTCTTTGCAGGCGAAGAGGTCCTTCTGGAATTCATAGAGAAAGTGAAATCAATGAAAGAGACGGGGCTTAAGGCTGAAGCTGTTTGGACAGATTATAGCAATAGATGGTTCACTCTTATGCACTCTACCAGGCCTTTGATCTTCAAGGACCATCAAGAAATTGCAGAGCATGTTAGCCTCAACTAGAAACTTTCTACATTTTGATGCAAAAACTTATTTTTATTTTTTTCTGGTTTATGTTTATCTTAGCCACCTAGTTATCCAGGCTGCAGCTGTGCTGGAGGGTGTTCGAGATGTAACAGTGGCTTCACATCTTATCGGAGACATGAGTGGATCTACCATCGAAGATCCACTCTCAGATCGATATGAGAAACTGGGTGGTTCTGTTTCTCTCCTGGAGAAGGATTCAGATGACTACAAAATGATCGTTAACTATCTGGAGAAAACCTATGAGCCTGTGAAAGTTGGAGACATTGTAAGAGCTGGCATTCAGTTAAATACTTGAAATATTTCAAAGAATGTTGGTTTTTGAGGTTGGCTTTTTGAACCTGTGTTTTCCAGGAATATGGAGTGAGTGTTGACAACATTTTTACGGTAGAGTCGACAGCTCTCCCCAGCTACGAAGAAATAAAGAAACTACCAAACAAAGTCCTATTGTGGTGTGGTGGGTTGGCCTAGTCCTGTTAATTAACCATGACTTCAAGGACGATGATCGTTTTTTTCTATCTAAATTCCCAACTTTCTTCACAGGCACCCGAACTTCAAACCTCTTGAGGCACTTGTACAAAGGCTTCTTGCCAGCCATATGCTCTCTTCCAGTCTCGGGTTACATGGTACTCAGCAATTGCAATCTTTTCTTAAAACACCAAAAGAAACCTCAATGTGGATTCTCATTTTGTTTTGTAGTTTGGCCGAGCGATTGTGTGCTCTGATGCTGCAGCAGAGGCAGCTAGGTATGGGTTTACGGCCGTAGACAGGCCAGAAGGGTTTTTGGTCCTGGCCATTGCATCTCTTGGAGAGGAGATCACAGAGATGACAAGCCCACCTGAGGTAAGTGTATAAACTTGATTTTGCGGTGTTCCTGCACATTCAGCAGGTGGTTAAATTAATCGGCACGCGGCTGGTACAGGATACAAAATCGTTGGAAGAGAAGAAGGTAGGAGTTAAAGCATTGGGGAGAAAGAAGACAGACGAATCAGAACACTTTGTTTGGAAAGATGACATTAAAGTTCCTTGTGGCTCTTTGATCGCTTCAGAACACAAAGATAGCCCTCTTGAATACAACGAGTATGCTGTCTACGACCCAAAGCAGGTATGCCTCTCTTACAAACTGCTACTACGTTCTTTCTCATCAAAGAAAAACCTATATCAGGAGCACATTCTCGCACATGCATAGCATTAATTTCCTGGTGAAACTAATTGAAATAAGTTTAAAAAGAAAAGAAACGAAACAAAATGAATGGAGTCCTCTCGTTTTTTATAATTGGCATTTATTTGGCAAGTCCATAAAATTAAACCCGTTTTCGGGTCCTATACCTTGCAGACAAGTATAAGGTTCTTGGTGGGGGTGAAGTACGAAGAGAAAGGTGTGGTGATGGAAACACAGGAA

mRNA sequence

GTAAAAATTTTGAAGGTTCATGAAACGAGGTCCCATTCTCACACTCATGGTTCTGGTGAAGAAGAAAAGAAAGTGACAAGGAAGCAGAAGGCAGAGAGCAAGACACATGAAAGCGAACTTTCGCCAAAGAAAGTCAAGTCCGAAGATGAAAATAGCCGCCCCAACGGCAAATCCGAAGTCAGCCTTGCAGATGAATTTGAAGAGTTCTGTAAGGCTACGAGAGAGAGTCTCTCCATTGATCAAATGAAGGAGATTCTGCAAATGAATGACCAAGACTCCACTGGCCCTGACTATGAAGTTGCTTCTAAATGTCAAGACTTGCTGTTCTATGGTGTCCTAGAAACATGCCCAGTTTGTAGTGGCAAGCTGGTATTTGATGGGAAGCGCTACTCTTGCAATGGCTTTCTAAGTGAATGGTCTACCTGTACCTTCAAAACGAAGGATCCTCCTAGGAAAAATGAAACCAGCAAGTTACCAGAATCAGTCTTGAATTCTCCCATTTCAGATTTGATAAAGAAGCATCAGAATCCGGGTCAACGTCCAAAGAGAAATCTTGGCCAGACAGATAAACCCTTCTCTGGAATGACAATTTCACTCTCTGGTCGTCTATCGAGAACTCATCATTACTGGAGAAAAGAAATTGAGAAGCATGGAGGGACCGTTAACAATTCTGTAATTGGTGTCAGTTGCTTGGTTGTCTCTCCTGCTGAGAGAGAGCGTGGTGGATCTTCTAAAGTTGCAGAAGCAGTCGAAACAGGTATACGAGTCATCCGTGAAGCTTGGCTACTTGACAGCATTGAGAAGCAAGAAGCTCAGCCATTGGAAGCTTATGATGTTTTGACAGATCTCGCTGTGGAAGGCAAAGGGATCCCTTGGGATAAACAAGATCCCTCGGAAGAGGCACTTGAATCCCTCACAGCAGAACTCAAGTTATATGGAAAGAGAGGGGTTTACAAGGATACCAAATTACGAGAACAGGGTGGAGAGATATTTGAAAAGGATGGAATCTTGTATAATTGTGCCTTCTCACTCTGCGATCAAGGCCGGGGACTCAATGAGTATTGTATTGCTCAACTGATCAAAGTACCAGATAAGAACTTGCATCTATACTATAAGAAAGGAAACGTGGGGGATGATCCCAATGCAGAGGAAAGGCTGGAGGAATGGGAAAATGAGAAAACTGCTATAAAGGAGTTTGTGGGGCTGTTCGAAGAAATAACTGGAAATGAATTCGAGCCATGGGAAAGAGAGAAGAAGTTTGAGAAGAAACGCTTGTCGTTTTATCCCATCGATATGGATGATGGAGTAGATGTGAGGCATGGAGGGCTTGGTCTTAGGCAGCTAGGAGTTGCATCTGTTCACAGTAAGCTTGAGCCCGCGATTGCAAACTTTATGAAGGTTTTGTGCAGCCAGGAGATTTACAAGTATGCTCTCATGGAGATGGCACATGATCCTCCGGAATTACCTATGGGAATGCTTTCTAATCTTCACTTGAAGAGATGCGAAGAGGTCCTTCTGGAATTCATAGAGAAAGTGAAATCAATGAAAGAGACGGGGCTTAAGGCTGAAGCTGTTTGGACAGATTATAGCAATAGATGGTTCACTCTTATGCACTCTACCAGGCCTTTGATCTTCAAGGACCATCAAGAAATTGCAGAGCATGCTGCAGCTGTGCTGGAGGGTGTTCGAGATGTAACAGTGGCTTCACATCTTATCGGAGACATGAGTGGATCTACCATCGAAGATCCACTCTCAGATCGATATGAGAAACTGGGTGGTTCTGTTTCTCTCCTGGAGAAGGATTCAGATGACTACAAAATGATCGTTAACTATCTGGAGAAAACCTATGAGCCTGTGAAAGTTGGAGACATTGAATATGGAGTGAGTGTTGACAACATTTTTACGGTAGAGTCGACAGCTCTCCCCAGCTACGAAGAAATAAAGAAACTACCAAACAAAGTCCTATTGTGGTGTGGCACCCGAACTTCAAACCTCTTGAGGCACTTGTACAAAGGCTTCTTGCCAGCCATATGCTCTCTTCCAGTCTCGGGTTACATGTTTGGCCGAGCGATTGTGTGCTCTGATGCTGCAGCAGAGGCAGCTAGGTATGGGTTTACGGCCGTAGACAGGCCAGAAGGGTTTTTGGTCCTGGCCATTGCATCTCTTGGAGAGGAGATCACAGAGATGACAAGCCCACCTGAGGATACAAAATCGTTGGAAGAGAAGAAGGTAGGAGTTAAAGCATTGGGGAGAAAGAAGACAGACGAATCAGAACACTTTGTTTGGAAAGATGACATTAAAGTTCCTTGTGGCTCTTTGATCGCTTCAGAACACAAAGATAGCCCTCTTGAATACAACGAGTATGCTGTCTACGACCCAAAGCAGACAAGTATAAGGTTCTTGGTGGGGGTGAAGTACGAAGAGAAAGGTGTGGTGATGGAAACACAGGAA

Coding sequence (CDS)

GTAAAAATTTTGAAGGTTCATGAAACGAGGTCCCATTCTCACACTCATGGTTCTGGTGAAGAAGAAAAGAAAGTGACAAGGAAGCAGAAGGCAGAGAGCAAGACACATGAAAGCGAACTTTCGCCAAAGAAAGTCAAGTCCGAAGATGAAAATAGCCGCCCCAACGGCAAATCCGAAGTCAGCCTTGCAGATGAATTTGAAGAGTTCTGTAAGGCTACGAGAGAGAGTCTCTCCATTGATCAAATGAAGGAGATTCTGCAAATGAATGACCAAGACTCCACTGGCCCTGACTATGAAGTTGCTTCTAAATGTCAAGACTTGCTGTTCTATGGTGTCCTAGAAACATGCCCAGTTTGTAGTGGCAAGCTGGTATTTGATGGGAAGCGCTACTCTTGCAATGGCTTTCTAAGTGAATGGTCTACCTGTACCTTCAAAACGAAGGATCCTCCTAGGAAAAATGAAACCAGCAAGTTACCAGAATCAGTCTTGAATTCTCCCATTTCAGATTTGATAAAGAAGCATCAGAATCCGGGTCAACGTCCAAAGAGAAATCTTGGCCAGACAGATAAACCCTTCTCTGGAATGACAATTTCACTCTCTGGTCGTCTATCGAGAACTCATCATTACTGGAGAAAAGAAATTGAGAAGCATGGAGGGACCGTTAACAATTCTGTAATTGGTGTCAGTTGCTTGGTTGTCTCTCCTGCTGAGAGAGAGCGTGGTGGATCTTCTAAAGTTGCAGAAGCAGTCGAAACAGGTATACGAGTCATCCGTGAAGCTTGGCTACTTGACAGCATTGAGAAGCAAGAAGCTCAGCCATTGGAAGCTTATGATGTTTTGACAGATCTCGCTGTGGAAGGCAAAGGGATCCCTTGGGATAAACAAGATCCCTCGGAAGAGGCACTTGAATCCCTCACAGCAGAACTCAAGTTATATGGAAAGAGAGGGGTTTACAAGGATACCAAATTACGAGAACAGGGTGGAGAGATATTTGAAAAGGATGGAATCTTGTATAATTGTGCCTTCTCACTCTGCGATCAAGGCCGGGGACTCAATGAGTATTGTATTGCTCAACTGATCAAAGTACCAGATAAGAACTTGCATCTATACTATAAGAAAGGAAACGTGGGGGATGATCCCAATGCAGAGGAAAGGCTGGAGGAATGGGAAAATGAGAAAACTGCTATAAAGGAGTTTGTGGGGCTGTTCGAAGAAATAACTGGAAATGAATTCGAGCCATGGGAAAGAGAGAAGAAGTTTGAGAAGAAACGCTTGTCGTTTTATCCCATCGATATGGATGATGGAGTAGATGTGAGGCATGGAGGGCTTGGTCTTAGGCAGCTAGGAGTTGCATCTGTTCACAGTAAGCTTGAGCCCGCGATTGCAAACTTTATGAAGGTTTTGTGCAGCCAGGAGATTTACAAGTATGCTCTCATGGAGATGGCACATGATCCTCCGGAATTACCTATGGGAATGCTTTCTAATCTTCACTTGAAGAGATGCGAAGAGGTCCTTCTGGAATTCATAGAGAAAGTGAAATCAATGAAAGAGACGGGGCTTAAGGCTGAAGCTGTTTGGACAGATTATAGCAATAGATGGTTCACTCTTATGCACTCTACCAGGCCTTTGATCTTCAAGGACCATCAAGAAATTGCAGAGCATGCTGCAGCTGTGCTGGAGGGTGTTCGAGATGTAACAGTGGCTTCACATCTTATCGGAGACATGAGTGGATCTACCATCGAAGATCCACTCTCAGATCGATATGAGAAACTGGGTGGTTCTGTTTCTCTCCTGGAGAAGGATTCAGATGACTACAAAATGATCGTTAACTATCTGGAGAAAACCTATGAGCCTGTGAAAGTTGGAGACATTGAATATGGAGTGAGTGTTGACAACATTTTTACGGTAGAGTCGACAGCTCTCCCCAGCTACGAAGAAATAAAGAAACTACCAAACAAAGTCCTATTGTGGTGTGGCACCCGAACTTCAAACCTCTTGAGGCACTTGTACAAAGGCTTCTTGCCAGCCATATGCTCTCTTCCAGTCTCGGGTTACATGTTTGGCCGAGCGATTGTGTGCTCTGATGCTGCAGCAGAGGCAGCTAGGTATGGGTTTACGGCCGTAGACAGGCCAGAAGGGTTTTTGGTCCTGGCCATTGCATCTCTTGGAGAGGAGATCACAGAGATGACAAGCCCACCTGAGGATACAAAATCGTTGGAAGAGAAGAAGGTAGGAGTTAAAGCATTGGGGAGAAAGAAGACAGACGAATCAGAACACTTTGTTTGGAAAGATGACATTAAAGTTCCTTGTGGCTCTTTGATCGCTTCAGAACACAAAGATAGCCCTCTTGAATACAACGAGTATGCTGTCTACGACCCAAAGCAGACAAGTATAAGGTTCTTGGTGGGGGTGAAGTACGAAGAGAAAGGTGTGGTGATGGAAACACAGGAA

Protein sequence

VKILKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLADEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKLVFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKRNLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGSSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVPDKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAHDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPLIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTSNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLGEEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPLEYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE
Homology
BLAST of MC02g0197 vs. ExPASy Swiss-Prot
Match: Q9SWB4 (Protein ADP-ribosyltransferase PARP3 OS=Glycine max OX=3847 GN=PARP3 PE=2 SV=1)

HSP 1 Score: 1258.8 bits (3256), Expect = 0.0e+00
Identity = 605/817 (74.05%), Postives = 705/817 (86.29%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLA 63
           +KV ETR  SH H  GEEEK +TRKQKAESK HE E SPKK K E E+   NGKSE  +A
Sbjct: 1   MKVQETR--SHVHALGEEEKVMTRKQKAESKAHEVEHSPKKAKVEKEDGHINGKSETGVA 60

Query: 64  DEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 123
           +E++EFCKAT E L ++QM++IL+ N  DS+G D E+  +CQDLLFYG L+ C VC+G L
Sbjct: 61  EEYDEFCKATTEHLPLEQMRDILEANGLDSSGSDLEITRRCQDLLFYGALDKCSVCNGSL 120

Query: 124 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 183
            FDG+RY C GF SEW++CTF T++PPRK E  KLP+SV NS  SDL+KK+Q+P  RP R
Sbjct: 121 EFDGRRYVCRGFYSEWASCTFSTRNPPRKQEPIKLPDSVQNSLASDLLKKYQDPSHRPHR 180

Query: 184 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 243
           +LG  +KPF+GM ISL GRL+RTHHYW+  IEKHGG V NS+IG +CLV SPAERERGG+
Sbjct: 181 DLGLAEKPFTGMMISLMGRLTRTHHYWKTTIEKHGGKVANSIIGSTCLVASPAERERGGT 240

Query: 244 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 303
           SK+AEA+E  I V+REAWL+DSIEKQE QPLEAYD+++DL+V+GKGIPWDKQDP EEA+E
Sbjct: 241 SKLAEAMERSIPVVREAWLIDSIEKQEPQPLEAYDLVSDLSVDGKGIPWDKQDPGEEAIE 300

Query: 304 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 363
           SL+AELKLYGKRGVYKDTKL+EQGG+IFE+DGILYNCAFS+CDQGRGLN+YC+ QLI VP
Sbjct: 301 SLSAELKLYGKRGVYKDTKLQEQGGKIFERDGILYNCAFSVCDQGRGLNDYCVMQLIVVP 360

Query: 364 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKK 423
           +  LHLY+KKG VGDDPNAEERLEEW+N   A+KEFV LFEEITGNEFEPWEREKKF+KK
Sbjct: 361 ENRLHLYFKKGRVGDDPNAEERLEEWDNVDGALKEFVRLFEEITGNEFEPWEREKKFQKK 420

Query: 424 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 483
            L FYPIDMDDG++VRHG LGLRQLG+A+ H KLEP +ANFMKVLCSQEIYKYALMEM +
Sbjct: 421 PLKFYPIDMDDGIEVRHGALGLRQLGIAATHCKLEPLVANFMKVLCSQEIYKYALMEMGY 480

Query: 484 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 543
           D P+LP+GM++NLHLK+CE+VLLEFI+KVKS+KETG KAEAVWTD+S RWFTLMHSTRP 
Sbjct: 481 DCPDLPIGMVTNLHLKKCEDVLLEFIDKVKSLKETGPKAEAVWTDFSQRWFTLMHSTRPF 540

Query: 544 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 603
            F+D+QEIA+HAAA LEGVRD+T ASHLIGDM+GSTI+DPLS+ Y+KLG S+S L+K SD
Sbjct: 541 NFRDYQEIADHAAAALEGVRDITQASHLIGDMTGSTIDDPLSETYKKLGCSISALDKSSD 600

Query: 604 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 663
           DY+MIV YLEKTYEPVKVGDIEYGVSV+NIF V++   PSYE+I KLPNKVLLWCG+R+S
Sbjct: 601 DYEMIVKYLEKTYEPVKVGDIEYGVSVENIFAVQTGGCPSYEDIIKLPNKVLLWCGSRSS 660

Query: 664 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 723
           NLLRHL KGFLPAICSLP+ GYMFG+AIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG
Sbjct: 661 NLLRHLQKGFLPAICSLPIPGYMFGKAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 720

Query: 724 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 783
            EITE+ +PPED  SLEEKKVGVK  G+KKTDESEHFVWKDDIKVPCG L+AS+H+DSPL
Sbjct: 721 NEITELKTPPEDASSLEEKKVGVKGPGKKKTDESEHFVWKDDIKVPCGKLVASDHQDSPL 780

Query: 784 EYNEYAVYDPKQTSIRFLVGVKY---EEKGVVMETQE 818
           EYNEYAVYD K+  I +LVGVKY   EEKG V++T E
Sbjct: 781 EYNEYAVYDKKRARISYLVGVKYEEKEEKGAVIDTAE 815

BLAST of MC02g0197 vs. ExPASy Swiss-Prot
Match: Q1SGF1 (Protein ADP-ribosyltransferase PARP3 OS=Medicago truncatula OX=3880 GN=PARP3 PE=3 SV=1)

HSP 1 Score: 1236.1 bits (3197), Expect = 0.0e+00
Identity = 603/811 (74.35%), Postives = 700/811 (86.31%), Query Frame = 0

Query: 8   ETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDE-NSRPNGKSEVSLADEF 67
           E+RSH+  H  GEEEK +TRKQKAESK HE E SPKK K EDE N   NGKS   +  E+
Sbjct: 4   ESRSHNVHHAHGEEEKVMTRKQKAESKAHEVEHSPKKAKVEDEKNGHTNGKSASDVVQEY 63

Query: 68  EEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKLVFD 127
           +EFCKAT E LS++QMKEIL+ ND DS+G D E+  +CQDLLF+G LE C VC+G + FD
Sbjct: 64  DEFCKATNEQLSLEQMKEILEANDLDSSGSDLEITRRCQDLLFFGALEKCMVCNGNMEFD 123

Query: 128 GKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKRNLG 187
           G+RY C GF SEWS+CTF T++PPRK+E  KLP+SV NSP+SDL+KK+Q+P +RP+R+LG
Sbjct: 124 GRRYGCRGFYSEWSSCTFSTREPPRKDEPIKLPDSVQNSPVSDLLKKYQDPSKRPQRDLG 183

Query: 188 QTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGSSKV 247
              KPF+GM ISL GRL+RTH             +N S  G SCLV SPAER+RGG+SK+
Sbjct: 184 LAIKPFTGMMISLMGRLNRTH-------------LNFS--GASCLVASPAERDRGGTSKL 243

Query: 248 AEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALESLT 307
           A+A+E GI V+REAWL DSIEKQE QPLE+YD+++DL+V+GKGIPWDKQDP EEA+ESL+
Sbjct: 244 ADAMERGIPVVREAWLTDSIEKQEPQPLESYDLVSDLSVDGKGIPWDKQDPGEEAIESLS 303

Query: 308 AELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVPDKN 367
           AELKLYGKRGVYKDTKL EQ G+IFEKDGILYNCAFSLCDQGR LN+YC+ QLI VP+ +
Sbjct: 304 AELKLYGKRGVYKDTKLHEQDGKIFEKDGILYNCAFSLCDQGRKLNDYCVMQLIVVPENS 363

Query: 368 LHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKKRLS 427
           LHLY+KKG VGDDP+AEERLEE EN   AIKEFV LFEEITGNEFE WEREKKF+KK L 
Sbjct: 364 LHLYFKKGRVGDDPSAEERLEECENVDNAIKEFVRLFEEITGNEFESWEREKKFQKKPLK 423

Query: 428 FYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAHDPP 487
           FYPIDMDDGV+VRHG LGLRQLG+A+ H KLEP +ANFMKVLCSQEIYKYALMEM +D P
Sbjct: 424 FYPIDMDDGVEVRHGALGLRQLGIAATHCKLEPMVANFMKVLCSQEIYKYALMEMGYDSP 483

Query: 488 ELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPLIFK 547
           +LP+GM++NLHLKRCEE+LLEFIEKVK++KETG KA+A+W+D+S +WFTLMHSTRP IF+
Sbjct: 484 DLPIGMVTNLHLKRCEEILLEFIEKVKTLKETGPKADAIWSDFSQKWFTLMHSTRPFIFR 543

Query: 548 DHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSDDYK 607
           D+QEIA+HAAA LEGVRD+T+ASHLIGDMSGSTI+DPLSD Y+KLG S++ LEK+S+DY+
Sbjct: 544 DYQEIADHAAAALEGVRDITLASHLIGDMSGSTIDDPLSDTYKKLGCSITPLEKNSNDYE 603

Query: 608 MIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTSNLL 667
           MIV YLEKTYEPVKVGDIEYGVSV+NIFTVES+A PSY +I K+PNKVLLWCG+R+SNLL
Sbjct: 604 MIVKYLEKTYEPVKVGDIEYGVSVENIFTVESSACPSYADIVKMPNKVLLWCGSRSSNLL 663

Query: 668 RHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLGEEI 727
           RHL+KGFLPAICSLPV GYMFG+AIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG EI
Sbjct: 664 RHLHKGFLPAICSLPVPGYMFGKAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLGNEI 723

Query: 728 TEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPLEYN 787
           TE+ SPPEDT SLEEKKVGVK LG+KKTDESEHFVWKDDIKVPCGS+IASEH+DSPLEYN
Sbjct: 724 TELKSPPEDTTSLEEKKVGVKGLGKKKTDESEHFVWKDDIKVPCGSIIASEHEDSPLEYN 783

Query: 788 EYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 818
           EYAVYDPKQ  I +LVGVKYEEK  V++T E
Sbjct: 784 EYAVYDPKQVRISYLVGVKYEEKDAVIDTAE 799

BLAST of MC02g0197 vs. ExPASy Swiss-Prot
Match: Q9FK91 (Protein ADP-ribosyltransferase PARP3 OS=Arabidopsis thaliana OX=3702 GN=PARP3 PE=2 SV=2)

HSP 1 Score: 1201.0 bits (3106), Expect = 0.0e+00
Identity = 572/809 (70.70%), Postives = 686/809 (84.80%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRP-NGKSEVSL 63
           +KVHETRSH+H   SG+E+K   RK KAE K  ESE S KK K E+++ R  NG  +   
Sbjct: 1   MKVHETRSHAHM--SGDEQKGNLRKHKAEGKLPESEQSQKKAKPENDDGRSVNGAGDA-- 60

Query: 64  ADEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGK 123
           A E+ EFCKA  E+LSIDQ+KE+L++N QD + P+  + ++CQDLLFYG L  CP+C G 
Sbjct: 61  ASEYNEFCKAVEENLSIDQIKEVLEINGQDCSAPEETLLAQCQDLLFYGALAKCPLCGGT 120

Query: 124 LVFDG-KRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRP 183
           L+ D  KR+ C G +SEW +C F TKDPPRK E  K+P+SV+NS ISDLIKKHQ+P  RP
Sbjct: 121 LICDNEKRFVCGGEISEWCSCVFSTKDPPRKEEPVKIPDSVMNSAISDLIKKHQDPKSRP 180

Query: 184 KRNLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERG 243
           KR LG  DKPF GM ISL GRL+RTH YW+K+IE++GG V+N+V GV+CLVVSPAERERG
Sbjct: 181 KRELGSADKPFVGMMISLMGRLTRTHQYWKKKIERNGGKVSNTVQGVTCLVVSPAERERG 240

Query: 244 GSSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEA 303
           G+SK+ EA+E G+ V+ EAWL+DS+EK EAQPLEAYDV++DL+VEGKGIPWDKQDPSEEA
Sbjct: 241 GTSKMVEAMEQGLPVVSEAWLIDSVEKHEAQPLEAYDVVSDLSVEGKGIPWDKQDPSEEA 300

Query: 304 LESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIK 363
           +ES +AELK+YGKRGVY DTKL+E+GG+IFEKDG+LYNCAFS+CD G+G NEYCI QL+ 
Sbjct: 301 IESFSAELKMYGKRGVYMDTKLQERGGKIFEKDGLLYNCAFSICDLGKGRNEYCIMQLVT 360

Query: 364 VPDKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFE 423
           VPD NL++Y+K+G VGDDPNAEERLEEWE+E+ AIKEF  LFEEI GNEFEPWEREKK +
Sbjct: 361 VPDSNLNMYFKRGKVGDDPNAEERLEEWEDEEAAIKEFARLFEEIAGNEFEPWEREKKIQ 420

Query: 424 KKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEM 483
           KK   F+PIDMDDG++VR G LGLRQLG+AS H KL+  +ANF+KVLC QEIY YALME+
Sbjct: 421 KKPHKFFPIDMDDGIEVRSGALGLRQLGIASAHCKLDSFVANFIKVLCGQEIYNYALMEL 480

Query: 484 AHDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTR 543
             DPP+LPMGML+++HLKRCEEVLLEF+EKVK+ KETG KAEA+W D+S+RWF+LMHSTR
Sbjct: 481 GLDPPDLPMGMLTDIHLKRCEEVLLEFVEKVKTTKETGQKAEAMWADFSSRWFSLMHSTR 540

Query: 544 PLIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKD 603
           P+   D  E+A+HAA+  E VRD+  AS LIGDM G T++DPLSDRY+KLG  +S+++K+
Sbjct: 541 PMRLHDVNELADHAASAFETVRDINTASRLIGDMRGDTLDDPLSDRYKKLGCKISVVDKE 600

Query: 604 SDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTR 663
           S+DYKM+V YLE TYEPVKV D+EYGVSV N+F VES A+PS ++IKKLPNKVLLWCG+R
Sbjct: 601 SEDYKMVVKYLETTYEPVKVSDVEYGVSVQNVFAVESDAIPSLDDIKKLPNKVLLWCGSR 660

Query: 664 TSNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIAS 723
           +SNLLRH+YKGFLPA+CSLPV GYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLA+AS
Sbjct: 661 SSNLLRHIYKGFLPAVCSLPVPGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAVAS 720

Query: 724 LGEEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDS 783
           LGEE+TE TSPPEDTK+LE+KK+GVK LGRKKT+ESEHF+W+DDIKVPCG L+ SEHKDS
Sbjct: 721 LGEEVTEFTSPPEDTKTLEDKKIGVKGLGRKKTEESEHFMWRDDIKVPCGRLVPSEHKDS 780

Query: 784 PLEYNEYAVYDPKQTSIRFLVGVKYEEKG 811
           PLEYNEYAVYDPKQTSIRFLV VKYEEKG
Sbjct: 781 PLEYNEYAVYDPKQTSIRFLVEVKYEEKG 805

BLAST of MC02g0197 vs. ExPASy Swiss-Prot
Match: Q0E0Q3 (Protein ADP-ribosyltransferase PARP3 OS=Oryza sativa subsp. japonica OX=39947 GN=PARP3 PE=2 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 8.2e-279
Identity = 481/833 (57.74%), Postives = 619/833 (74.31%), Query Frame = 0

Query: 6   VHETRSHSHTHGSGEEEKKVTRKQKAESKTHE-SELSPKKVKSEDENSRPNGKSEVS--- 65
           VHETRS +    S EE K   +KQK ESK  E  + +P K K   +N   +G+ E S   
Sbjct: 2   VHETRSRT-LAASQEEGKAAPKKQKTESKEQEGGQQAPSKNKKTADNEEHDGEQEPSKNK 61

Query: 66  --------------LADEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDL 125
                            EF EFCKA RE L+I+ M++ILQ N+QD++G +  V  +C+D+
Sbjct: 62  KLKAEESDLNGKATAVKEFSEFCKAIREHLTIEDMRKILQGNEQDASGSEDAVVPRCEDV 121

Query: 126 LFYGVLETCPVCSGKLVFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPI 185
           +FYG L+ CPVC G+L   G +Y+C G  SEW+ C+F T +P R+    K+P+ V N  +
Sbjct: 122 MFYGPLDKCPVCGGQLECKGLKYNCTGTHSEWACCSFSTNNPSRRGGPIKVPDDVKNDFV 181

Query: 186 SDLIKKHQNPGQRPKRNLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIG 245
              +K+ Q   + PKRNL   +  FSGM I+LSGR+SR+H Y++++I KHGG VNNSVIG
Sbjct: 182 RKWLKQ-QEGNKYPKRNL-DDEGIFSGMMIALSGRMSRSHGYFKEQIMKHGGKVNNSVIG 241

Query: 246 VSCLVVSPAERERGGSSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEG 305
           V+C+V SPAER +GGS   AEA+E G  V+ E W++DS++K+E QPL AYD+ +D+  EG
Sbjct: 242 VTCVVASPAERHQGGSGGFAEALERGTPVVSENWIIDSVQKKEKQPLAAYDIASDVVPEG 301

Query: 306 KGIPWDKQDPSEEALESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQ 365
           +G+P    DP+EEA+E+L AELKL GKR V+KD+KL + GG I+EKDGI+YNCAFS+CD 
Sbjct: 302 RGLPLGNLDPTEEAIETLAAELKLAGKRAVHKDSKLEKDGGHIYEKDGIIYNCAFSVCDL 361

Query: 366 GRGLNEYCIAQLIKVPDKNLHLYYKKGNVGDDPNAEERLEEWENE-KTAIKEFVGLFEEI 425
           G  +N+ CI QLI VP+ +LHLYYKKG +G D  AEER+E++ +    AIKEFV LFEE+
Sbjct: 362 GGDINQLCIMQLIMVPENHLHLYYKKGPIGHDQMAEERVEDFGSRFNDAIKEFVRLFEEV 421

Query: 426 TGNEFEPWEREKKFEKKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMK 485
           TGNEFEPWEREKKF+KK +  YP+DMDDGVDVRHGG+ LRQLG A+ H KL+P++   MK
Sbjct: 422 TGNEFEPWEREKKFKKKCMKMYPLDMDDGVDVRHGGVALRQLGAAAAHCKLDPSVTFIMK 481

Query: 486 VLCSQEIYKYALMEMAHDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVW 545
            LCSQEIY+YAL EM HD P+LP+GML++LHLKR EE LLE+ + V+S  E+G  A+A W
Sbjct: 482 QLCSQEIYRYALTEMGHDVPDLPIGMLTDLHLKRGEETLLEWKQDVESAPESGPAADAFW 541

Query: 546 TDYSNRWFTLMHSTRPLIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSD 605
            + SN+WFTL  +TRP   K +++IA++ A+ LE VRD+ VAS LIGD+ GST++DPLS 
Sbjct: 542 MEISNKWFTLFPTTRPYTMKGYEQIADNVASGLETVRDINVASRLIGDVFGSTLDDPLSQ 601

Query: 606 RYEKLGGSVSLLEKDSDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEE 665
            Y+KLG S++ + +DS+DYKMI+ YLEKTYEPVKVGD+ Y  +V+ I+ VES+ALPSY+E
Sbjct: 602 CYKKLGCSINRVVEDSEDYKMILKYLEKTYEPVKVGDVVYSATVERIYAVESSALPSYDE 661

Query: 666 IKKLPNKVLLWCGTRTSNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFT 725
           IKKLPNKVLLWCGTR+SNLLRHL  GF+PA+C +PV GYMFG+AIVCSDAAAEAA YGFT
Sbjct: 662 IKKLPNKVLLWCGTRSSNLLRHLRDGFVPAVCHIPVPGYMFGKAIVCSDAAAEAALYGFT 721

Query: 726 AVDRPEGFLVLAIASLGEEITEMTSPP--EDTKSLEEKKVGVKALGRKKTDESEHFVWKD 785
           AVDRPEG+LVLA+ASLG+EI E+T  P  ED K +EEKK+GVK +GRK TD SEHF W+D
Sbjct: 722 AVDRPEGYLVLAVASLGKEIQEITGTPGSEDVKRMEEKKMGVKGVGRKTTDPSEHFTWRD 781

Query: 786 DIKVPCGSLIASEHKDSPLEYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 818
            + VPCG L+ S +KD PLEYNEYAVYDPKQ SI FLVGVKYEE+ + +   E
Sbjct: 782 GVTVPCGKLVPSTNKDGPLEYNEYAVYDPKQVSIAFLVGVKYEEQNMEVVPDE 831

BLAST of MC02g0197 vs. ExPASy Swiss-Prot
Match: Q9ZP54 (Poly [ADP-ribose] polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=PARP1 PE=1 SV=2)

HSP 1 Score: 344.0 bits (881), Expect = 4.7e-93
Identity = 249/833 (29.89%), Postives = 417/833 (50.06%), Query Frame = 0

Query: 13  SHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLADEFEEFCKA 72
           + T  + +   +   K+K +S  +E     K  KS  + S        S   E E   K 
Sbjct: 199 TETAEARQTNSRAGTKRKNDSVDNEKS---KLAKSSFDMSTSGALQPCSKEKEMEAQTKE 258

Query: 73  -------TRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKLVF 132
                   ++ ++  +++E+L++N+Q + G + ++  KC D + +G L  CP+CSG L F
Sbjct: 259 LWDLKDDLKKYVTSAELREMLEVNEQSTRGSELDLRDKCADGMMFGPLALCPMCSGHLSF 318

Query: 133 DGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKRNL 192
            G  Y C+G++SEWS C+  T DP R     K+P+   N  +    K  ++   +PKR L
Sbjct: 319 SGGLYRCHGYISEWSKCSHSTLDPDRIKGKWKIPDETENQFLLKWNKSQKS--VKPKRIL 378

Query: 193 ----------GQTDKP---------FSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSV- 252
                     GQ  K           + + +S++G  ++    W+K IE+ G   + +V 
Sbjct: 379 RPVLSGETSQGQGSKDATDSSRSERLADLKVSIAGN-TKERQPWKKRIEEAGAEFHANVK 438

Query: 253 IGVSCLVVSPAERERGGSSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAV 312
            G SCLVV      R    + A  ++  + ++RE +L+D  +KQ   P + Y +      
Sbjct: 439 KGTSCLVVCGLTDIRDAEMRKARRMK--VAIVREDYLVDCFKKQRKLPFDKYKI------ 498

Query: 313 EGKGIPWDKQDPSEEALESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLC 372
                        E+  ESL   +K+ G+  V++ + L+E    I E    +YN   S+ 
Sbjct: 499 -------------EDTSESLVT-VKVKGRSAVHEASGLQEH-CHILEDGNSIYNTTLSMS 558

Query: 373 DQGRGLNEYCIAQLIKVPDK--NLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLF 432
           D   G+N Y I Q+I+  DK  + +++ K G VG++     ++EE  ++  A+ EF  LF
Sbjct: 559 DLSTGINSYYILQIIQ-EDKGSDCYVFRKWGRVGNEKIGGNKVEEM-SKSDAVHEFKRLF 618

Query: 433 EEITGNEFEPWEREKKFEKKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIAN 492
            E TGN +E WE++  F+K+   F P+D+D GV+ +     + +       S L P++  
Sbjct: 619 LEKTGNTWESWEQKTNFQKQPGKFLPLDIDYGVNKQ-----VAKKEPFQTSSNLAPSLIE 678

Query: 493 FMKVLCSQEIYKYALMEMAHDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAE 552
            MK+L   E Y+ A+ME   +  E+P+G LS  ++++  E L E    +          E
Sbjct: 679 LMKMLFDVETYRSAMMEFEINMSEMPLGKLSKHNIQKGFEALTEIQRLLTESDPQPTMKE 738

Query: 553 AVWTDYSNRWFTLMHSTRPLIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDP 612
           ++  D SNR+FT++ S  P I +D  +  +    +LE ++D+ +AS ++G    ST  + 
Sbjct: 739 SLLVDASNRFFTMIPSIHPHIIRDEDDF-KSKVKMLEALQDIEIASRIVGFDVDST--ES 798

Query: 613 LSDRYEKLGGSVSLLEKDSDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTA-LP 672
           L D+Y+KL   +S L  DS+DY++I  YL  T+ P      E+ + ++ +F +E      
Sbjct: 799 LDDKYKKLHCDISPLPHDSEDYRLIEKYLNTTHAPTHT---EWSLELEEVFALEREGEFD 858

Query: 673 SY-EEIKKLPNKVLLWCGTRTSNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAA 732
            Y    +KL NK+LLW G+R +N +  L +G   A    P +GYMFG+ I  +D  +++A
Sbjct: 859 KYAPHREKLGNKMLLWHGSRLTNFVGILNQGLRIAPPEAPATGYMFGKGIYFADLVSKSA 918

Query: 733 RYGFTAVDRPEGFLVLAIASLGE--EITE---MTSPPEDTKSLEEKKVGVKALGRKKTDE 792
           +Y +T    P G ++L+  +LGE  E+T+   M  PP         K   K LG+K   +
Sbjct: 919 QYCYTCKKNPVGLMLLSEVALGEIHELTKAKYMDKPPRG-------KHSTKGLGKKVPQD 978

Query: 793 SEHFVWKDDIKVPCGSLIASEHKDSPLEYNEYAVYDPKQTSIRFLVGVKYEEK 810
           SE   W+ D+ VPCG  ++S+ K S L YNEY VYD  Q  ++FL+ V+++ K
Sbjct: 979 SEFAKWRGDVTVPCGKPVSSKVKASELMYNEYIVYDTAQVKLQFLLKVRFKHK 982

BLAST of MC02g0197 vs. NCBI nr
Match: XP_022153011.1 (poly [ADP-ribose] polymerase 3 [Momordica charantia])

HSP 1 Score: 1618 bits (4191), Expect = 0.0
Identity = 809/814 (99.39%), Postives = 811/814 (99.63%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLA 63
           +KVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESE SPKKVKSEDENSRPNGKSEVSLA
Sbjct: 1   MKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESEQSPKKVKSEDENSRPNGKSEVSLA 60

Query: 64  DEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 123
           DEFEEFCKATRESLSI+QMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL
Sbjct: 61  DEFEEFCKATRESLSINQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 120

Query: 124 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 183
           VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR
Sbjct: 121 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 180

Query: 184 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 243
           NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS
Sbjct: 181 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 240

Query: 244 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 303
           SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE
Sbjct: 241 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 300

Query: 304 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 363
           SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP
Sbjct: 301 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 360

Query: 364 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKK 423
           DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREK FEKK
Sbjct: 361 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREK-FEKK 420

Query: 424 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 483
           RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH
Sbjct: 421 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 480

Query: 484 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 543
           DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL
Sbjct: 481 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 540

Query: 544 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 603
           IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD
Sbjct: 541 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 600

Query: 604 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 663
           DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS
Sbjct: 601 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 660

Query: 664 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 723
           NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLA ASLG
Sbjct: 661 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAXASLG 720

Query: 724 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 783
           EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL
Sbjct: 721 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 780

Query: 784 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE
Sbjct: 781 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 813

BLAST of MC02g0197 vs. NCBI nr
Match: KAG7035937.1 (Poly [ADP-ribose] polymerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1528 bits (3955), Expect = 0.0
Identity = 752/817 (92.04%), Postives = 786/817 (96.21%), Query Frame = 0

Query: 1   VKILKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEV 60
           V+ILKVHE RSHSH HGSGEEEK +TRKQKAESKTHE+E SPKKVKSEDEN RPN KSE 
Sbjct: 6   VQILKVHEKRSHSHAHGSGEEEKIMTRKQKAESKTHENEQSPKKVKSEDENGRPNCKSET 65

Query: 61  SLADEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCS 120
            LADEFE FCKATRES+SIDQMKEILQMN+QDSTGPDYEVASKCQDLLFYG LE CPVCS
Sbjct: 66  DLADEFESFCKATRESISIDQMKEILQMNEQDSTGPDYEVASKCQDLLFYGALEGCPVCS 125

Query: 121 GKLVFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQR 180
           G LVFDGKRY+CNGFLSEWSTCTFKTKDPPRKNET+KLPESVLNSPISDLIKK+QNPGQR
Sbjct: 126 GNLVFDGKRYACNGFLSEWSTCTFKTKDPPRKNETNKLPESVLNSPISDLIKKYQNPGQR 185

Query: 181 PKRNLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERER 240
            KRNLGQ+DKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERER
Sbjct: 186 RKRNLGQSDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERER 245

Query: 241 GGSSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEE 300
           GGSSKVAEAVE GIRV+REAWLLDSIEKQEAQPLEAYD++TDL VEGKGIPWDKQDPSEE
Sbjct: 246 GGSSKVAEAVERGIRVVREAWLLDSIEKQEAQPLEAYDIVTDLTVEGKGIPWDKQDPSEE 305

Query: 301 ALESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLI 360
           ALESLTAELKLYGKRGVYKDTKL+EQGGEIFEKDGILYNCAFSLCDQGRGLNEYCI QLI
Sbjct: 306 ALESLTAELKLYGKRGVYKDTKLQEQGGEIFEKDGILYNCAFSLCDQGRGLNEYCITQLI 365

Query: 361 KVPDKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKF 420
           KVPDKNLHL+YKKG VGD+PNAEERLEEWENE  AIKEFV LFEEITGNEFEPWEREKKF
Sbjct: 366 KVPDKNLHLFYKKGKVGDNPNAEERLEEWENEGNAIKEFVRLFEEITGNEFEPWEREKKF 425

Query: 421 EKKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALME 480
           EKKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEP +ANFMKVLCSQEIYKYALME
Sbjct: 426 EKKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPVVANFMKVLCSQEIYKYALME 485

Query: 481 MAHDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHST 540
           M HDPPE PMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHST
Sbjct: 486 MGHDPPEFPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHST 545

Query: 541 RPLIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEK 600
           RPLIFKD+QEIAEHAAAVLE VRD+TVASHLIGDM+GSTI+DPLSDRY+KLGGS+SLL+K
Sbjct: 546 RPLIFKDYQEIAEHAAAVLESVRDITVASHLIGDMTGSTIDDPLSDRYKKLGGSISLLDK 605

Query: 601 DSDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGT 660
           DSDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIF+VES ALPSYEEIKKLPNKVLLWCGT
Sbjct: 606 DSDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFSVESAALPSYEEIKKLPNKVLLWCGT 665

Query: 661 RTSNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIA 720
           R+SNLLRHL+KGFLPAICSLPV GYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAI 
Sbjct: 666 RSSNLLRHLHKGFLPAICSLPVPGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIV 725

Query: 721 SLGEEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKD 780
           SLGEE+ E+TSPPEDTKSLEEKK+GVKALGRKKTDESEHFVWKDDI+VPCG+LIASEHKD
Sbjct: 726 SLGEEMIEITSPPEDTKSLEEKKMGVKALGRKKTDESEHFVWKDDIRVPCGTLIASEHKD 785

Query: 781 SPLEYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           SPLEYNEY+VYD KQ SIRFLVGVKYEEK VVM+T+E
Sbjct: 786 SPLEYNEYSVYDTKQASIRFLVGVKYEEKEVVMDTKE 822

BLAST of MC02g0197 vs. NCBI nr
Match: XP_022958062.1 (poly [ADP-ribose] polymerase 3 [Cucurbita moschata])

HSP 1 Score: 1519 bits (3934), Expect = 0.0
Identity = 748/814 (91.89%), Postives = 782/814 (96.07%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLA 63
           +KVHE RSHSH HGSGEEEK +TRKQKAESKTHE+E SPKKVKSEDEN  PN KSE  LA
Sbjct: 1   MKVHEKRSHSHAHGSGEEEKIMTRKQKAESKTHENEQSPKKVKSEDENGCPNCKSETDLA 60

Query: 64  DEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 123
           DEFE FCKATRES+SIDQMKEILQMN+QDSTGPDYEVASKCQDLLFYG LE CPVCSG L
Sbjct: 61  DEFESFCKATRESISIDQMKEILQMNEQDSTGPDYEVASKCQDLLFYGALEGCPVCSGNL 120

Query: 124 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 183
           VFDGKRY+CNGFLSEWSTCTFKTKDPPRKNET+KLPESVLNSPISDLIKK+QNPGQR KR
Sbjct: 121 VFDGKRYACNGFLSEWSTCTFKTKDPPRKNETNKLPESVLNSPISDLIKKYQNPGQRRKR 180

Query: 184 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 243
           NLGQ+DKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS
Sbjct: 181 NLGQSDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 240

Query: 244 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 303
           SKVAEAVE GIRV+REAWLLDSIEKQEAQPLEAYD++TDL VEGKGIPWDKQDPSEEALE
Sbjct: 241 SKVAEAVERGIRVVREAWLLDSIEKQEAQPLEAYDIVTDLTVEGKGIPWDKQDPSEEALE 300

Query: 304 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 363
           SLTAELKLYGKRGVYKDTKL+EQGGEIFEKDGILYNCAFSLCDQGRGLNEYCI QLIKVP
Sbjct: 301 SLTAELKLYGKRGVYKDTKLQEQGGEIFEKDGILYNCAFSLCDQGRGLNEYCITQLIKVP 360

Query: 364 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKK 423
           DKNLHL+YKKG VGD+PNAEERLEEWENE  AIKEFV LFEEITGNEFEPWEREKKFEKK
Sbjct: 361 DKNLHLFYKKGKVGDNPNAEERLEEWENEGNAIKEFVRLFEEITGNEFEPWEREKKFEKK 420

Query: 424 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 483
           RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEP +ANFMKVLCSQEIYKYALMEM H
Sbjct: 421 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPVVANFMKVLCSQEIYKYALMEMGH 480

Query: 484 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 543
           DPPE PMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL
Sbjct: 481 DPPEFPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 540

Query: 544 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 603
           IFKD+QEIAEHAAAVLE VRD+TVASHLIGDM+GSTI+DPLSDRY+KLGGS+SLL+KDSD
Sbjct: 541 IFKDYQEIAEHAAAVLESVRDITVASHLIGDMTGSTIDDPLSDRYKKLGGSISLLDKDSD 600

Query: 604 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 663
           DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIF+VES ALPSYEEIKKLPNKVLLWCGTR+S
Sbjct: 601 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFSVESAALPSYEEIKKLPNKVLLWCGTRSS 660

Query: 664 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 723
           NLLRHL+KGFLPAICSLPV GYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAI SLG
Sbjct: 661 NLLRHLHKGFLPAICSLPVPGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIVSLG 720

Query: 724 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 783
           EE+ E+TSPPEDTKSLEEKK+GVKALGRKKTDESEHFVWKDDI+VPCG+LIASEHKDSPL
Sbjct: 721 EEMIEITSPPEDTKSLEEKKMGVKALGRKKTDESEHFVWKDDIRVPCGTLIASEHKDSPL 780

Query: 784 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           EYNEY+VYD KQ SIRFLVGVKYEEK VVM+T+E
Sbjct: 781 EYNEYSVYDTKQASIRFLVGVKYEEKEVVMDTKE 814

BLAST of MC02g0197 vs. NCBI nr
Match: XP_023532794.1 (poly [ADP-ribose] polymerase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1515 bits (3922), Expect = 0.0
Identity = 745/814 (91.52%), Postives = 779/814 (95.70%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLA 63
           +KVHE RSHSH HGSGEEEK +TRKQKAESKTHE+E SPKKVKSEDEN RPN KSE  LA
Sbjct: 1   MKVHEKRSHSHAHGSGEEEKIMTRKQKAESKTHENEQSPKKVKSEDENGRPNCKSETDLA 60

Query: 64  DEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 123
           DEFE FC ATRES+SIDQMKEILQMN+QDSTGPDYEVASKCQDLLFYG LE CPVCSG L
Sbjct: 61  DEFESFCNATRESISIDQMKEILQMNEQDSTGPDYEVASKCQDLLFYGALEGCPVCSGNL 120

Query: 124 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 183
           VFDGKRY+CNGFLSEWSTCTFKTKDPPRKNET+KLPESVLNSPISDLIKK+QNPGQR KR
Sbjct: 121 VFDGKRYACNGFLSEWSTCTFKTKDPPRKNETNKLPESVLNSPISDLIKKYQNPGQRRKR 180

Query: 184 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 243
           NLGQ+DKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS
Sbjct: 181 NLGQSDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 240

Query: 244 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 303
           SKVAEAVE GIRV+REAWLLDSIEKQEAQPLEAYD++TDL VEGKGIPWDKQDP EEALE
Sbjct: 241 SKVAEAVERGIRVVREAWLLDSIEKQEAQPLEAYDIVTDLTVEGKGIPWDKQDPFEEALE 300

Query: 304 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 363
           SL AELKLYGKRGVYKDTKL+EQGGEIFEKDGILYNCAFSLCDQGRGLNEYCI QLIKVP
Sbjct: 301 SLAAELKLYGKRGVYKDTKLQEQGGEIFEKDGILYNCAFSLCDQGRGLNEYCITQLIKVP 360

Query: 364 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKK 423
           DKNLHL+YKKG VGD+PNAEERLEEWENE  AIKEFV LFEEITGNEFEPWEREKKFEKK
Sbjct: 361 DKNLHLFYKKGKVGDNPNAEERLEEWENEGDAIKEFVRLFEEITGNEFEPWEREKKFEKK 420

Query: 424 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 483
           RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEP +ANFMKVLCSQEIYKYALMEM H
Sbjct: 421 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPVVANFMKVLCSQEIYKYALMEMGH 480

Query: 484 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 543
           DPPE PMGMLSNLHLKRCEEVL+EFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL
Sbjct: 481 DPPEFPMGMLSNLHLKRCEEVLMEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 540

Query: 544 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 603
           IFKD+QEIAEHAAAVLE VRD+TVASHLIGDM+GSTI+DPLSDRY+KLGGS+SLLEKDSD
Sbjct: 541 IFKDYQEIAEHAAAVLESVRDITVASHLIGDMTGSTIDDPLSDRYKKLGGSISLLEKDSD 600

Query: 604 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 663
           DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIF+VES ALPSYEEIKKLPNKVLLWCGTR+S
Sbjct: 601 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFSVESEALPSYEEIKKLPNKVLLWCGTRSS 660

Query: 664 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 723
           NL+RHL+KGFLPAICSLPV GYMFGRAIVCSDAA EAARYGFTAVDRPEGFLVLAI SLG
Sbjct: 661 NLMRHLHKGFLPAICSLPVPGYMFGRAIVCSDAAGEAARYGFTAVDRPEGFLVLAIVSLG 720

Query: 724 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 783
           EEI E+TSPPEDTKSLEEKK+GVKALGRKKTDESEHFVWKDDI+VPCG+LIASEHKDSPL
Sbjct: 721 EEIIEITSPPEDTKSLEEKKMGVKALGRKKTDESEHFVWKDDIRVPCGTLIASEHKDSPL 780

Query: 784 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           EYNEY+VYD KQ SIRFLVGVKYEEK VVM+T+E
Sbjct: 781 EYNEYSVYDTKQASIRFLVGVKYEEKEVVMDTKE 814

BLAST of MC02g0197 vs. NCBI nr
Match: XP_022995025.1 (poly [ADP-ribose] polymerase 3 [Cucurbita maxima])

HSP 1 Score: 1512 bits (3915), Expect = 0.0
Identity = 745/814 (91.52%), Postives = 777/814 (95.45%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLA 63
           +KVHE RSHSH+HGSGEEEK +TRKQKAESKTHE+E SPKKVKSEDEN R N KSE  LA
Sbjct: 1   MKVHEKRSHSHSHGSGEEEKIMTRKQKAESKTHENEQSPKKVKSEDENGRLNCKSESDLA 60

Query: 64  DEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 123
           DEFE FCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYG LE CPVCSG L
Sbjct: 61  DEFESFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGALEGCPVCSGNL 120

Query: 124 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 183
           VFDGKRY+CNGFLSEWSTCTFKTKDPPRKNET+KLPESVLNSPISDLIKK+QNPGQRPKR
Sbjct: 121 VFDGKRYACNGFLSEWSTCTFKTKDPPRKNETNKLPESVLNSPISDLIKKYQNPGQRPKR 180

Query: 184 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 243
           NLGQ+DKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS
Sbjct: 181 NLGQSDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 240

Query: 244 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 303
           SKVAEAVE GIRV+REAWLLDSIEKQEAQPLEAYD++TDL VEGKG PWDKQDPSEEALE
Sbjct: 241 SKVAEAVERGIRVVREAWLLDSIEKQEAQPLEAYDIVTDLTVEGKGTPWDKQDPSEEALE 300

Query: 304 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 363
           SLTAELKLYGKRGVYKDTKL+EQGGEIFEKDGILYNCAFSLCDQGRGLNEYCI QLIKVP
Sbjct: 301 SLTAELKLYGKRGVYKDTKLQEQGGEIFEKDGILYNCAFSLCDQGRGLNEYCITQLIKVP 360

Query: 364 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKK 423
           DK LHL+YKKG VGDDPNA+ERLEEWENE+ AIKEFV LFEEITGNEFEPWEREKKFEKK
Sbjct: 361 DKKLHLFYKKGKVGDDPNAQERLEEWENEENAIKEFVRLFEEITGNEFEPWEREKKFEKK 420

Query: 424 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 483
           RLSFYPIDMDDGVDVRHGGLGLRQLG+ASVHSKLEP + NFMKVLCSQEIYKYALMEM H
Sbjct: 421 RLSFYPIDMDDGVDVRHGGLGLRQLGIASVHSKLEPVVGNFMKVLCSQEIYKYALMEMGH 480

Query: 484 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 543
           DPPE PMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWF+LMHSTRPL
Sbjct: 481 DPPEFPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFSLMHSTRPL 540

Query: 544 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 603
           IFKD+QEIAEHAAAVLE VRD+TVASHLIGDMSGSTI+DPLSDRY+KLGGS+SLLEKDSD
Sbjct: 541 IFKDYQEIAEHAAAVLESVRDITVASHLIGDMSGSTIDDPLSDRYKKLGGSISLLEKDSD 600

Query: 604 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 663
           DYKMIVNYLEKTYEPVKVGDIEYG SVDNIF+VES ALPSYEEIKKLPNKVLLWCGTR+S
Sbjct: 601 DYKMIVNYLEKTYEPVKVGDIEYGASVDNIFSVESAALPSYEEIKKLPNKVLLWCGTRSS 660

Query: 664 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 723
           NLLRHL+KGFLPAICSLPV GYMFGRAIVC DAAAEAARYG TAVDRPEGFLVLAI SLG
Sbjct: 661 NLLRHLHKGFLPAICSLPVPGYMFGRAIVCCDAAAEAARYGLTAVDRPEGFLVLAIVSLG 720

Query: 724 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 783
           EEI E TSPPEDTKSLEEKK+GVKALGRKKTDESEHFVWKDDI+VPCG+LIASEHKDSPL
Sbjct: 721 EEIIERTSPPEDTKSLEEKKMGVKALGRKKTDESEHFVWKDDIRVPCGTLIASEHKDSPL 780

Query: 784 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           EYNEY+VYD KQ SIRFLV VKYEEK VVM+T+E
Sbjct: 781 EYNEYSVYDTKQASIRFLVRVKYEEKEVVMDTKE 814

BLAST of MC02g0197 vs. ExPASy TrEMBL
Match: A0A6J1DJF0 (Poly [ADP-ribose] polymerase OS=Momordica charantia OX=3673 GN=LOC111020617 PE=4 SV=1)

HSP 1 Score: 1618 bits (4191), Expect = 0.0
Identity = 809/814 (99.39%), Postives = 811/814 (99.63%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLA 63
           +KVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESE SPKKVKSEDENSRPNGKSEVSLA
Sbjct: 1   MKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESEQSPKKVKSEDENSRPNGKSEVSLA 60

Query: 64  DEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 123
           DEFEEFCKATRESLSI+QMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL
Sbjct: 61  DEFEEFCKATRESLSINQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 120

Query: 124 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 183
           VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR
Sbjct: 121 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 180

Query: 184 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 243
           NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS
Sbjct: 181 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 240

Query: 244 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 303
           SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE
Sbjct: 241 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 300

Query: 304 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 363
           SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP
Sbjct: 301 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 360

Query: 364 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKK 423
           DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREK FEKK
Sbjct: 361 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREK-FEKK 420

Query: 424 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 483
           RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH
Sbjct: 421 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 480

Query: 484 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 543
           DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL
Sbjct: 481 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 540

Query: 544 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 603
           IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD
Sbjct: 541 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 600

Query: 604 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 663
           DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS
Sbjct: 601 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 660

Query: 664 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 723
           NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLA ASLG
Sbjct: 661 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAXASLG 720

Query: 724 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 783
           EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL
Sbjct: 721 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 780

Query: 784 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE
Sbjct: 781 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 813

BLAST of MC02g0197 vs. ExPASy TrEMBL
Match: A0A6J1H0W3 (Poly [ADP-ribose] polymerase OS=Cucurbita moschata OX=3662 GN=LOC111459405 PE=4 SV=1)

HSP 1 Score: 1519 bits (3934), Expect = 0.0
Identity = 748/814 (91.89%), Postives = 782/814 (96.07%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLA 63
           +KVHE RSHSH HGSGEEEK +TRKQKAESKTHE+E SPKKVKSEDEN  PN KSE  LA
Sbjct: 1   MKVHEKRSHSHAHGSGEEEKIMTRKQKAESKTHENEQSPKKVKSEDENGCPNCKSETDLA 60

Query: 64  DEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 123
           DEFE FCKATRES+SIDQMKEILQMN+QDSTGPDYEVASKCQDLLFYG LE CPVCSG L
Sbjct: 61  DEFESFCKATRESISIDQMKEILQMNEQDSTGPDYEVASKCQDLLFYGALEGCPVCSGNL 120

Query: 124 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 183
           VFDGKRY+CNGFLSEWSTCTFKTKDPPRKNET+KLPESVLNSPISDLIKK+QNPGQR KR
Sbjct: 121 VFDGKRYACNGFLSEWSTCTFKTKDPPRKNETNKLPESVLNSPISDLIKKYQNPGQRRKR 180

Query: 184 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 243
           NLGQ+DKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS
Sbjct: 181 NLGQSDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 240

Query: 244 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 303
           SKVAEAVE GIRV+REAWLLDSIEKQEAQPLEAYD++TDL VEGKGIPWDKQDPSEEALE
Sbjct: 241 SKVAEAVERGIRVVREAWLLDSIEKQEAQPLEAYDIVTDLTVEGKGIPWDKQDPSEEALE 300

Query: 304 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 363
           SLTAELKLYGKRGVYKDTKL+EQGGEIFEKDGILYNCAFSLCDQGRGLNEYCI QLIKVP
Sbjct: 301 SLTAELKLYGKRGVYKDTKLQEQGGEIFEKDGILYNCAFSLCDQGRGLNEYCITQLIKVP 360

Query: 364 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKK 423
           DKNLHL+YKKG VGD+PNAEERLEEWENE  AIKEFV LFEEITGNEFEPWEREKKFEKK
Sbjct: 361 DKNLHLFYKKGKVGDNPNAEERLEEWENEGNAIKEFVRLFEEITGNEFEPWEREKKFEKK 420

Query: 424 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 483
           RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEP +ANFMKVLCSQEIYKYALMEM H
Sbjct: 421 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPVVANFMKVLCSQEIYKYALMEMGH 480

Query: 484 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 543
           DPPE PMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL
Sbjct: 481 DPPEFPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 540

Query: 544 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 603
           IFKD+QEIAEHAAAVLE VRD+TVASHLIGDM+GSTI+DPLSDRY+KLGGS+SLL+KDSD
Sbjct: 541 IFKDYQEIAEHAAAVLESVRDITVASHLIGDMTGSTIDDPLSDRYKKLGGSISLLDKDSD 600

Query: 604 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 663
           DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIF+VES ALPSYEEIKKLPNKVLLWCGTR+S
Sbjct: 601 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFSVESAALPSYEEIKKLPNKVLLWCGTRSS 660

Query: 664 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 723
           NLLRHL+KGFLPAICSLPV GYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAI SLG
Sbjct: 661 NLLRHLHKGFLPAICSLPVPGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIVSLG 720

Query: 724 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 783
           EE+ E+TSPPEDTKSLEEKK+GVKALGRKKTDESEHFVWKDDI+VPCG+LIASEHKDSPL
Sbjct: 721 EEMIEITSPPEDTKSLEEKKMGVKALGRKKTDESEHFVWKDDIRVPCGTLIASEHKDSPL 780

Query: 784 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           EYNEY+VYD KQ SIRFLVGVKYEEK VVM+T+E
Sbjct: 781 EYNEYSVYDTKQASIRFLVGVKYEEKEVVMDTKE 814

BLAST of MC02g0197 vs. ExPASy TrEMBL
Match: A0A6J1K2Y7 (Poly [ADP-ribose] polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490703 PE=4 SV=1)

HSP 1 Score: 1512 bits (3915), Expect = 0.0
Identity = 745/814 (91.52%), Postives = 777/814 (95.45%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLA 63
           +KVHE RSHSH+HGSGEEEK +TRKQKAESKTHE+E SPKKVKSEDEN R N KSE  LA
Sbjct: 1   MKVHEKRSHSHSHGSGEEEKIMTRKQKAESKTHENEQSPKKVKSEDENGRLNCKSESDLA 60

Query: 64  DEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKL 123
           DEFE FCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYG LE CPVCSG L
Sbjct: 61  DEFESFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGALEGCPVCSGNL 120

Query: 124 VFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKR 183
           VFDGKRY+CNGFLSEWSTCTFKTKDPPRKNET+KLPESVLNSPISDLIKK+QNPGQRPKR
Sbjct: 121 VFDGKRYACNGFLSEWSTCTFKTKDPPRKNETNKLPESVLNSPISDLIKKYQNPGQRPKR 180

Query: 184 NLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 243
           NLGQ+DKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS
Sbjct: 181 NLGQSDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGGS 240

Query: 244 SKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEALE 303
           SKVAEAVE GIRV+REAWLLDSIEKQEAQPLEAYD++TDL VEGKG PWDKQDPSEEALE
Sbjct: 241 SKVAEAVERGIRVVREAWLLDSIEKQEAQPLEAYDIVTDLTVEGKGTPWDKQDPSEEALE 300

Query: 304 SLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKVP 363
           SLTAELKLYGKRGVYKDTKL+EQGGEIFEKDGILYNCAFSLCDQGRGLNEYCI QLIKVP
Sbjct: 301 SLTAELKLYGKRGVYKDTKLQEQGGEIFEKDGILYNCAFSLCDQGRGLNEYCITQLIKVP 360

Query: 364 DKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEKK 423
           DK LHL+YKKG VGDDPNA+ERLEEWENE+ AIKEFV LFEEITGNEFEPWEREKKFEKK
Sbjct: 361 DKKLHLFYKKGKVGDDPNAQERLEEWENEENAIKEFVRLFEEITGNEFEPWEREKKFEKK 420

Query: 424 RLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMAH 483
           RLSFYPIDMDDGVDVRHGGLGLRQLG+ASVHSKLEP + NFMKVLCSQEIYKYALMEM H
Sbjct: 421 RLSFYPIDMDDGVDVRHGGLGLRQLGIASVHSKLEPVVGNFMKVLCSQEIYKYALMEMGH 480

Query: 484 DPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRPL 543
           DPPE PMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWF+LMHSTRPL
Sbjct: 481 DPPEFPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFSLMHSTRPL 540

Query: 544 IFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDSD 603
           IFKD+QEIAEHAAAVLE VRD+TVASHLIGDMSGSTI+DPLSDRY+KLGGS+SLLEKDSD
Sbjct: 541 IFKDYQEIAEHAAAVLESVRDITVASHLIGDMSGSTIDDPLSDRYKKLGGSISLLEKDSD 600

Query: 604 DYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRTS 663
           DYKMIVNYLEKTYEPVKVGDIEYG SVDNIF+VES ALPSYEEIKKLPNKVLLWCGTR+S
Sbjct: 601 DYKMIVNYLEKTYEPVKVGDIEYGASVDNIFSVESAALPSYEEIKKLPNKVLLWCGTRSS 660

Query: 664 NLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASLG 723
           NLLRHL+KGFLPAICSLPV GYMFGRAIVC DAAAEAARYG TAVDRPEGFLVLAI SLG
Sbjct: 661 NLLRHLHKGFLPAICSLPVPGYMFGRAIVCCDAAAEAARYGLTAVDRPEGFLVLAIVSLG 720

Query: 724 EEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSPL 783
           EEI E TSPPEDTKSLEEKK+GVKALGRKKTDESEHFVWKDDI+VPCG+LIASEHKDSPL
Sbjct: 721 EEIIERTSPPEDTKSLEEKKMGVKALGRKKTDESEHFVWKDDIRVPCGTLIASEHKDSPL 780

Query: 784 EYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           EYNEY+VYD KQ SIRFLV VKYEEK VVM+T+E
Sbjct: 781 EYNEYSVYDTKQASIRFLVRVKYEEKEVVMDTKE 814

BLAST of MC02g0197 vs. ExPASy TrEMBL
Match: A0A0A0KBU1 (Poly [ADP-ribose] polymerase OS=Cucumis sativus OX=3659 GN=Csa_6G152930 PE=4 SV=1)

HSP 1 Score: 1504 bits (3894), Expect = 0.0
Identity = 744/815 (91.29%), Postives = 780/815 (95.71%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVS-L 63
           +KVHETRSHSHTHG+GEEEK +TRKQKAESKTHE+E SPKK+KSEDEN R NGKSE S L
Sbjct: 1   MKVHETRSHSHTHGAGEEEKIMTRKQKAESKTHENEQSPKKIKSEDENGRANGKSEASDL 60

Query: 64  ADEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGK 123
             EFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYE+ASKCQDLLFYG LETCPVC G+
Sbjct: 61  TKEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEIASKCQDLLFYGALETCPVCRGR 120

Query: 124 LVFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPK 183
           LVFDGKRY CNGFLSEWSTCTFKTKDPPR+NET+KLPESVLNSP+SDLIKK+QNP QRPK
Sbjct: 121 LVFDGKRYVCNGFLSEWSTCTFKTKDPPRRNETNKLPESVLNSPVSDLIKKYQNPSQRPK 180

Query: 184 RNLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGG 243
           RNLGQ+DKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGG
Sbjct: 181 RNLGQSDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGG 240

Query: 244 SSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEAL 303
           SSKVAEAVE GIRVIREAWLLDSIEKQEAQPLEAYDV+TDLAVEGKGIPWDKQDPSEEAL
Sbjct: 241 SSKVAEAVERGIRVIREAWLLDSIEKQEAQPLEAYDVVTDLAVEGKGIPWDKQDPSEEAL 300

Query: 304 ESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKV 363
           ESLTAELKLYGKRGV+KDTKL+EQGGEIFEKD ILYNCAFSLCDQGRGLNEYCI QLIKV
Sbjct: 301 ESLTAELKLYGKRGVHKDTKLQEQGGEIFEKDRILYNCAFSLCDQGRGLNEYCITQLIKV 360

Query: 364 PDKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEK 423
           PDKNLHL+YKKG VGDDPNAEERLEEWENE  A+KEFV LF+EITGNEFE WEREKKFEK
Sbjct: 361 PDKNLHLFYKKGKVGDDPNAEERLEEWENEGNAVKEFVRLFKEITGNEFELWEREKKFEK 420

Query: 424 KRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMA 483
           KR SF+PIDMDDGVDVRHGGLGLRQLG+A+VHSKLEP IANFMKVLCSQEIYKYALMEMA
Sbjct: 421 KRGSFHPIDMDDGVDVRHGGLGLRQLGIATVHSKLEPEIANFMKVLCSQEIYKYALMEMA 480

Query: 484 HDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRP 543
           HDPPELPMGMLS  HL+RCEE+LLEFIEKVKSMKETG KAEAVWTDYSNRWFTLMHSTRP
Sbjct: 481 HDPPELPMGMLSKFHLERCEEILLEFIEKVKSMKETGFKAEAVWTDYSNRWFTLMHSTRP 540

Query: 544 LIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDS 603
           LIFKD+QEIAEHAAAVLEGVRDVTVASHLIGDMSGSTI+DPLSDRY KLGGSVSLLEKDS
Sbjct: 541 LIFKDYQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIDDPLSDRYGKLGGSVSLLEKDS 600

Query: 604 DDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRT 663
           DDYKMIVNYLEKTYEPVKVGDIEYGV+VDNIF+VES ALPSY EIKK PNKVLLWCGTR+
Sbjct: 601 DDYKMIVNYLEKTYEPVKVGDIEYGVTVDNIFSVESAALPSYGEIKKQPNKVLLWCGTRS 660

Query: 664 SNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASL 723
           SNLLRHL+KGFLPAICSLPV GYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAI SL
Sbjct: 661 SNLLRHLHKGFLPAICSLPVPGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIVSL 720

Query: 724 GEEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSP 783
           GEEITE+TS PEDTK LEEKK+GVKALGRKKTDESEHFVWKD+I+VPCG LI SEHKDSP
Sbjct: 721 GEEITEITSLPEDTKLLEEKKMGVKALGRKKTDESEHFVWKDEIRVPCGPLITSEHKDSP 780

Query: 784 LEYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           LEYNEYAVY+PKQT+IRFLVGVKYEEKGVVM+TQE
Sbjct: 781 LEYNEYAVYNPKQTNIRFLVGVKYEEKGVVMDTQE 815

BLAST of MC02g0197 vs. ExPASy TrEMBL
Match: A0A1S4E1X9 (Poly [ADP-ribose] polymerase OS=Cucumis melo OX=3656 GN=LOC103498021 PE=4 SV=1)

HSP 1 Score: 1501 bits (3885), Expect = 0.0
Identity = 743/815 (91.17%), Postives = 779/815 (95.58%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVS-L 63
           +KVH+TRSHSH HG+GEEEK +TRKQKAESKTHE+E SPKKVKSEDEN R NGKSE S L
Sbjct: 1   MKVHDTRSHSHAHGAGEEEKIMTRKQKAESKTHENEQSPKKVKSEDENGRANGKSEASDL 60

Query: 64  ADEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGK 123
            +EFEEFCKATRESLSIDQMKEILQMNDQDSTG DYE+ASKCQDLLFYG LETCPVC G+
Sbjct: 61  TNEFEEFCKATRESLSIDQMKEILQMNDQDSTGSDYEIASKCQDLLFYGALETCPVCRGR 120

Query: 124 LVFDGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPK 183
           LVFDGKRY CNGFLSEWSTCTFKTKDPPRKNET+KLPESVLNSP+SDLIKK QNP QRPK
Sbjct: 121 LVFDGKRYFCNGFLSEWSTCTFKTKDPPRKNETNKLPESVLNSPVSDLIKKCQNPSQRPK 180

Query: 184 RNLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGG 243
           RNLGQ+DKPFS MTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGG
Sbjct: 181 RNLGQSDKPFSRMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERGG 240

Query: 244 SSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEAL 303
           SSKVAEAVE GIRVIREAWLLDSIEKQEAQPLEAYDV+TDLAVEGKGIPWDKQDPSEEAL
Sbjct: 241 SSKVAEAVERGIRVIREAWLLDSIEKQEAQPLEAYDVVTDLAVEGKGIPWDKQDPSEEAL 300

Query: 304 ESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIKV 363
           ESLTAELKLYGKRGV+KDTKL+EQGG+IFEKDGILYNCAFSLCDQGRGLNEYCI QLIKV
Sbjct: 301 ESLTAELKLYGKRGVHKDTKLQEQGGKIFEKDGILYNCAFSLCDQGRGLNEYCITQLIKV 360

Query: 364 PDKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFEK 423
           PDKNLHL+YKKG VGDDPNAEERLEEWENE+ A+KEFV LFEEITGNEFE WEREKKFEK
Sbjct: 361 PDKNLHLFYKKGKVGDDPNAEERLEEWENEENAVKEFVRLFEEITGNEFELWEREKKFEK 420

Query: 424 KRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEMA 483
           KRLSFYPIDMDDGVDVRHGGLGLRQLG+A+VHSKLEP IANFMKVLCSQEIYKYALMEMA
Sbjct: 421 KRLSFYPIDMDDGVDVRHGGLGLRQLGIATVHSKLEPEIANFMKVLCSQEIYKYALMEMA 480

Query: 484 HDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTRP 543
           HDPPE PMGMLS  HL+RCEEVLLEFI KVKSMKETG KAEAVWTDYSNRWFTLMHSTRP
Sbjct: 481 HDPPEFPMGMLSKFHLERCEEVLLEFIGKVKSMKETGFKAEAVWTDYSNRWFTLMHSTRP 540

Query: 544 LIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKDS 603
           LIFKD+QEIAEHAAAVLEGVRDVTVASHLIGDMSGSTI+DPLSDRY KLGG+VSLLEKDS
Sbjct: 541 LIFKDYQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIDDPLSDRYGKLGGTVSLLEKDS 600

Query: 604 DDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTRT 663
           DDY+MIVNYLEKTYEPVKVGDIEYGV+VDNIF+VES ALPSYEEIKK PNKVLLWCGTR+
Sbjct: 601 DDYRMIVNYLEKTYEPVKVGDIEYGVTVDNIFSVESAALPSYEEIKKQPNKVLLWCGTRS 660

Query: 664 SNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIASL 723
           SNLLRHL+KGFLPAICSLPV GYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAI SL
Sbjct: 661 SNLLRHLHKGFLPAICSLPVPGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIVSL 720

Query: 724 GEEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDSP 783
           GEEITE+TS PEDTKSLEEKK+GVKALGRKKTDESEHFVWKD+I+VPCG LIASEHKDSP
Sbjct: 721 GEEITEITSLPEDTKSLEEKKMGVKALGRKKTDESEHFVWKDEIRVPCGPLIASEHKDSP 780

Query: 784 LEYNEYAVYDPKQTSIRFLVGVKYEEKGVVMETQE 817
           LEYNEYAVY+PKQT+IRFLVGVKYEEK  VM+TQE
Sbjct: 781 LEYNEYAVYNPKQTNIRFLVGVKYEEKDAVMDTQE 815

BLAST of MC02g0197 vs. TAIR 10
Match: AT5G22470.1 (NAD+ ADP-ribosyltransferases;NAD+ ADP-ribosyltransferases )

HSP 1 Score: 1201.0 bits (3106), Expect = 0.0e+00
Identity = 572/809 (70.70%), Postives = 686/809 (84.80%), Query Frame = 0

Query: 4   LKVHETRSHSHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRP-NGKSEVSL 63
           +KVHETRSH+H   SG+E+K   RK KAE K  ESE S KK K E+++ R  NG  +   
Sbjct: 1   MKVHETRSHAHM--SGDEQKGNLRKHKAEGKLPESEQSQKKAKPENDDGRSVNGAGDA-- 60

Query: 64  ADEFEEFCKATRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGK 123
           A E+ EFCKA  E+LSIDQ+KE+L++N QD + P+  + ++CQDLLFYG L  CP+C G 
Sbjct: 61  ASEYNEFCKAVEENLSIDQIKEVLEINGQDCSAPEETLLAQCQDLLFYGALAKCPLCGGT 120

Query: 124 LVFDG-KRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRP 183
           L+ D  KR+ C G +SEW +C F TKDPPRK E  K+P+SV+NS ISDLIKKHQ+P  RP
Sbjct: 121 LICDNEKRFVCGGEISEWCSCVFSTKDPPRKEEPVKIPDSVMNSAISDLIKKHQDPKSRP 180

Query: 184 KRNLGQTDKPFSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSVIGVSCLVVSPAERERG 243
           KR LG  DKPF GM ISL GRL+RTH YW+K+IE++GG V+N+V GV+CLVVSPAERERG
Sbjct: 181 KRELGSADKPFVGMMISLMGRLTRTHQYWKKKIERNGGKVSNTVQGVTCLVVSPAERERG 240

Query: 244 GSSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAVEGKGIPWDKQDPSEEA 303
           G+SK+ EA+E G+ V+ EAWL+DS+EK EAQPLEAYDV++DL+VEGKGIPWDKQDPSEEA
Sbjct: 241 GTSKMVEAMEQGLPVVSEAWLIDSVEKHEAQPLEAYDVVSDLSVEGKGIPWDKQDPSEEA 300

Query: 304 LESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLCDQGRGLNEYCIAQLIK 363
           +ES +AELK+YGKRGVY DTKL+E+GG+IFEKDG+LYNCAFS+CD G+G NEYCI QL+ 
Sbjct: 301 IESFSAELKMYGKRGVYMDTKLQERGGKIFEKDGLLYNCAFSICDLGKGRNEYCIMQLVT 360

Query: 364 VPDKNLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLFEEITGNEFEPWEREKKFE 423
           VPD NL++Y+K+G VGDDPNAEERLEEWE+E+ AIKEF  LFEEI GNEFEPWEREKK +
Sbjct: 361 VPDSNLNMYFKRGKVGDDPNAEERLEEWEDEEAAIKEFARLFEEIAGNEFEPWEREKKIQ 420

Query: 424 KKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIANFMKVLCSQEIYKYALMEM 483
           KK   F+PIDMDDG++VR G LGLRQLG+AS H KL+  +ANF+KVLC QEIY YALME+
Sbjct: 421 KKPHKFFPIDMDDGIEVRSGALGLRQLGIASAHCKLDSFVANFIKVLCGQEIYNYALMEL 480

Query: 484 AHDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAVWTDYSNRWFTLMHSTR 543
             DPP+LPMGML+++HLKRCEEVLLEF+EKVK+ KETG KAEA+W D+S+RWF+LMHSTR
Sbjct: 481 GLDPPDLPMGMLTDIHLKRCEEVLLEFVEKVKTTKETGQKAEAMWADFSSRWFSLMHSTR 540

Query: 544 PLIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDPLSDRYEKLGGSVSLLEKD 603
           P+   D  E+A+HAA+  E VRD+  AS LIGDM G T++DPLSDRY+KLG  +S+++K+
Sbjct: 541 PMRLHDVNELADHAASAFETVRDINTASRLIGDMRGDTLDDPLSDRYKKLGCKISVVDKE 600

Query: 604 SDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTALPSYEEIKKLPNKVLLWCGTR 663
           S+DYKM+V YLE TYEPVKV D+EYGVSV N+F VES A+PS ++IKKLPNKVLLWCG+R
Sbjct: 601 SEDYKMVVKYLETTYEPVKVSDVEYGVSVQNVFAVESDAIPSLDDIKKLPNKVLLWCGSR 660

Query: 664 TSNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAIAS 723
           +SNLLRH+YKGFLPA+CSLPV GYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLA+AS
Sbjct: 661 SSNLLRHIYKGFLPAVCSLPVPGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAVAS 720

Query: 724 LGEEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEHFVWKDDIKVPCGSLIASEHKDS 783
           LGEE+TE TSPPEDTK+LE+KK+GVK LGRKKT+ESEHF+W+DDIKVPCG L+ SEHKDS
Sbjct: 721 LGEEVTEFTSPPEDTKTLEDKKIGVKGLGRKKTEESEHFMWRDDIKVPCGRLVPSEHKDS 780

Query: 784 PLEYNEYAVYDPKQTSIRFLVGVKYEEKG 811
           PLEYNEYAVYDPKQTSIRFLV VKYEEKG
Sbjct: 781 PLEYNEYAVYDPKQTSIRFLVEVKYEEKG 805

BLAST of MC02g0197 vs. TAIR 10
Match: AT2G31320.1 (poly(ADP-ribose) polymerase 2 )

HSP 1 Score: 344.0 bits (881), Expect = 3.4e-94
Identity = 249/833 (29.89%), Postives = 417/833 (50.06%), Query Frame = 0

Query: 13  SHTHGSGEEEKKVTRKQKAESKTHESELSPKKVKSEDENSRPNGKSEVSLADEFEEFCKA 72
           + T  + +   +   K+K +S  +E     K  KS  + S        S   E E   K 
Sbjct: 199 TETAEARQTNSRAGTKRKNDSVDNEKS---KLAKSSFDMSTSGALQPCSKEKEMEAQTKE 258

Query: 73  -------TRESLSIDQMKEILQMNDQDSTGPDYEVASKCQDLLFYGVLETCPVCSGKLVF 132
                   ++ ++  +++E+L++N+Q + G + ++  KC D + +G L  CP+CSG L F
Sbjct: 259 LWDLKDDLKKYVTSAELREMLEVNEQSTRGSELDLRDKCADGMMFGPLALCPMCSGHLSF 318

Query: 133 DGKRYSCNGFLSEWSTCTFKTKDPPRKNETSKLPESVLNSPISDLIKKHQNPGQRPKRNL 192
            G  Y C+G++SEWS C+  T DP R     K+P+   N  +    K  ++   +PKR L
Sbjct: 319 SGGLYRCHGYISEWSKCSHSTLDPDRIKGKWKIPDETENQFLLKWNKSQKS--VKPKRIL 378

Query: 193 ----------GQTDKP---------FSGMTISLSGRLSRTHHYWRKEIEKHGGTVNNSV- 252
                     GQ  K           + + +S++G  ++    W+K IE+ G   + +V 
Sbjct: 379 RPVLSGETSQGQGSKDATDSSRSERLADLKVSIAGN-TKERQPWKKRIEEAGAEFHANVK 438

Query: 253 IGVSCLVVSPAERERGGSSKVAEAVETGIRVIREAWLLDSIEKQEAQPLEAYDVLTDLAV 312
            G SCLVV      R    + A  ++  + ++RE +L+D  +KQ   P + Y +      
Sbjct: 439 KGTSCLVVCGLTDIRDAEMRKARRMK--VAIVREDYLVDCFKKQRKLPFDKYKI------ 498

Query: 313 EGKGIPWDKQDPSEEALESLTAELKLYGKRGVYKDTKLREQGGEIFEKDGILYNCAFSLC 372
                        E+  ESL   +K+ G+  V++ + L+E    I E    +YN   S+ 
Sbjct: 499 -------------EDTSESLVT-VKVKGRSAVHEASGLQEH-CHILEDGNSIYNTTLSMS 558

Query: 373 DQGRGLNEYCIAQLIKVPDK--NLHLYYKKGNVGDDPNAEERLEEWENEKTAIKEFVGLF 432
           D   G+N Y I Q+I+  DK  + +++ K G VG++     ++EE  ++  A+ EF  LF
Sbjct: 559 DLSTGINSYYILQIIQ-EDKGSDCYVFRKWGRVGNEKIGGNKVEEM-SKSDAVHEFKRLF 618

Query: 433 EEITGNEFEPWEREKKFEKKRLSFYPIDMDDGVDVRHGGLGLRQLGVASVHSKLEPAIAN 492
            E TGN +E WE++  F+K+   F P+D+D GV+ +     + +       S L P++  
Sbjct: 619 LEKTGNTWESWEQKTNFQKQPGKFLPLDIDYGVNKQ-----VAKKEPFQTSSNLAPSLIE 678

Query: 493 FMKVLCSQEIYKYALMEMAHDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAE 552
            MK+L   E Y+ A+ME   +  E+P+G LS  ++++  E L E    +          E
Sbjct: 679 LMKMLFDVETYRSAMMEFEINMSEMPLGKLSKHNIQKGFEALTEIQRLLTESDPQPTMKE 738

Query: 553 AVWTDYSNRWFTLMHSTRPLIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTIEDP 612
           ++  D SNR+FT++ S  P I +D  +  +    +LE ++D+ +AS ++G    ST  + 
Sbjct: 739 SLLVDASNRFFTMIPSIHPHIIRDEDDF-KSKVKMLEALQDIEIASRIVGFDVDST--ES 798

Query: 613 LSDRYEKLGGSVSLLEKDSDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTVESTA-LP 672
           L D+Y+KL   +S L  DS+DY++I  YL  T+ P      E+ + ++ +F +E      
Sbjct: 799 LDDKYKKLHCDISPLPHDSEDYRLIEKYLNTTHAPTHT---EWSLELEEVFALEREGEFD 858

Query: 673 SY-EEIKKLPNKVLLWCGTRTSNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAEAA 732
            Y    +KL NK+LLW G+R +N +  L +G   A    P +GYMFG+ I  +D  +++A
Sbjct: 859 KYAPHREKLGNKMLLWHGSRLTNFVGILNQGLRIAPPEAPATGYMFGKGIYFADLVSKSA 918

Query: 733 RYGFTAVDRPEGFLVLAIASLGE--EITE---MTSPPEDTKSLEEKKVGVKALGRKKTDE 792
           +Y +T    P G ++L+  +LGE  E+T+   M  PP         K   K LG+K   +
Sbjct: 919 QYCYTCKKNPVGLMLLSEVALGEIHELTKAKYMDKPPRG-------KHSTKGLGKKVPQD 978

Query: 793 SEHFVWKDDIKVPCGSLIASEHKDSPLEYNEYAVYDPKQTSIRFLVGVKYEEK 810
           SE   W+ D+ VPCG  ++S+ K S L YNEY VYD  Q  ++FL+ V+++ K
Sbjct: 979 SEFAKWRGDVTVPCGKPVSSKVKASELMYNEYIVYDTAQVKLQFLLKVRFKHK 982

BLAST of MC02g0197 vs. TAIR 10
Match: AT4G02390.1 (poly(ADP-ribose) polymerase )

HSP 1 Score: 147.9 bits (372), Expect = 3.5e-35
Identity = 111/470 (23.62%), Postives = 221/470 (47.02%), Query Frame = 0

Query: 352 NEYCIAQLIKVPDKNLHLYYKK-GNVGDDPNAEERLE-EWENEKTAIKEFVGLFEEITGN 411
           N++ + Q+++   K  ++ Y + G VG     + +L+  +++   AI+ F   F + T N
Sbjct: 183 NKFFVLQVLESDSKKTYMVYTRWGRVG--VKGQSKLDGPYDSWDRAIEIFTNKFNDKTKN 242

Query: 412 EFEPWEREKKFEKKRLSFYPIDMDDGVDVR----HGGLGLRQLGVASVHSKLEPAIANFM 471
               W   K+F     S+  ++MD G +      +  +      V    SKL+  +A F+
Sbjct: 243 Y---WSDRKEFIPHPKSYTWLEMDYGKEENDSPVNNDIPSSSSEVKPEQSKLDTRVAKFI 302

Query: 472 KVLCSQEIYKYALMEMAHDPPELPMGMLSNLHLKRCEEVLLEFIEKVKSMKETGLKAEAV 531
            ++C+  +    +ME+ ++  +LP+G +S   + +  EVL    E +     T L+    
Sbjct: 303 SLICNVSMMAQHMMEIGYNANKLPLGKISKSTISKGYEVLKRISEVIDRYDRTRLE---- 362

Query: 532 WTDYSNRWFTLM-----HSTRPLIFKDHQEIAEHAAAVLEGVRDVTVASHLIGDMSGSTI 591
             + S  ++T++              D  +  +    ++E + ++ +A+ L+    G   
Sbjct: 363 --ELSGEFYTVIPHDFGFKKMSQFVIDTPQKLKQKIEMVEALGEIELATKLLSVDPGLQ- 422

Query: 592 EDPLSDRYEKLGGSVSLLEKDSDDYKMIVNYLEKTYEPVKVGDIEYGVSVDNIFTV-EST 651
           +DPL   Y++L   ++ +  DS+++ M+ NY+E T+     G   Y V +  +F    + 
Sbjct: 423 DDPLYYHYQQLNCGLTPVGNDSEEFSMVANYMENTHAKTHSG---YTVEIAQLFRASRAV 482

Query: 652 ALPSYEEIKKLPNKVLLWCGTRTSNLLRHLYKGFLPAICSLPVSGYMFGRAIVCSDAAAE 711
               +++     N++LLW G+R +N    L +G   A    PV+GYMFG+ +  +D  ++
Sbjct: 483 EADRFQQFSSSKNRMLLWHGSRLTNWAGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSK 542

Query: 712 AARYGFTAVDRPEGFLVLAIASLGEEITEMTSPPEDTKSLEEKKVGVKALGRKKTDESEH 771
           +A Y +      +G L+L   +LG ++ E+     +  +L   K+  K +G+   + SE 
Sbjct: 543 SANYCYANTGANDGVLLLCEVALG-DMNELLYSDYNADNLPPGKLSTKGVGKTAPNPSEA 602

Query: 772 FVWKDDIKVPCGSLIASEHKDSPLEYNEYAVYDPKQTSIRFLVGVKYEEK 810
              +D + VP G  +        L YNEY VY+ +Q  +R+++ VK+  K
Sbjct: 603 QTLEDGVVVPLGKPVERSCSKGMLLYNEYIVYNVEQIKMRYVIQVKFNYK 636

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SWB40.0e+0074.05Protein ADP-ribosyltransferase PARP3 OS=Glycine max OX=3847 GN=PARP3 PE=2 SV=1[more]
Q1SGF10.0e+0074.35Protein ADP-ribosyltransferase PARP3 OS=Medicago truncatula OX=3880 GN=PARP3 PE=... [more]
Q9FK910.0e+0070.70Protein ADP-ribosyltransferase PARP3 OS=Arabidopsis thaliana OX=3702 GN=PARP3 PE... [more]
Q0E0Q38.2e-27957.74Protein ADP-ribosyltransferase PARP3 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9ZP544.7e-9329.89Poly [ADP-ribose] polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=PARP1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_022153011.10.099.39poly [ADP-ribose] polymerase 3 [Momordica charantia][more]
KAG7035937.10.092.04Poly [ADP-ribose] polymerase 3, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022958062.10.091.89poly [ADP-ribose] polymerase 3 [Cucurbita moschata][more]
XP_023532794.10.091.52poly [ADP-ribose] polymerase 3 [Cucurbita pepo subsp. pepo][more]
XP_022995025.10.091.52poly [ADP-ribose] polymerase 3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1DJF00.099.39Poly [ADP-ribose] polymerase OS=Momordica charantia OX=3673 GN=LOC111020617 PE=4... [more]
A0A6J1H0W30.091.89Poly [ADP-ribose] polymerase OS=Cucurbita moschata OX=3662 GN=LOC111459405 PE=4 ... [more]
A0A6J1K2Y70.091.52Poly [ADP-ribose] polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490703 PE=4 SV... [more]
A0A0A0KBU10.091.29Poly [ADP-ribose] polymerase OS=Cucumis sativus OX=3659 GN=Csa_6G152930 PE=4 SV=... [more]
A0A1S4E1X90.091.17Poly [ADP-ribose] polymerase OS=Cucumis melo OX=3656 GN=LOC103498021 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G22470.10.0e+0070.70NAD+ ADP-ribosyltransferases;NAD+ ADP-ribosyltransferases [more]
AT2G31320.13.4e-9429.89poly(ADP-ribose) polymerase 2 [more]
AT4G02390.13.5e-3523.62poly(ADP-ribose) polymerase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001357BRCT domainSMARTSM00292BRCT_7coord: 189..269
e-value: 4.9E-12
score: 56.0
IPR001357BRCT domainPFAMPF00533BRCTcoord: 189..266
e-value: 2.1E-8
score: 34.4
IPR001357BRCT domainPROSITEPS50172BRCTcoord: 187..279
score: 17.34568
IPR012982PADR1 domainSMARTSM01335PADR1_2coord: 100..153
e-value: 1.9E-22
score: 90.6
IPR012982PADR1 domainPFAMPF08063PADR1coord: 103..152
e-value: 7.7E-20
score: 70.4
IPR008893WGR domainSMARTSM00773WGR_clscoord: 338..421
e-value: 2.4E-19
score: 80.3
IPR008893WGR domainPFAMPF05406WGRcoord: 338..417
e-value: 2.7E-11
score: 43.4
IPR004102Poly(ADP-ribose) polymerase, regulatory domainPFAMPF02877PARP_regcoord: 455..588
e-value: 9.4E-21
score: 74.3
IPR004102Poly(ADP-ribose) polymerase, regulatory domainPROSITEPS51060PARP_ALPHA_HDcoord: 454..573
score: 20.819633
IPR012317Poly(ADP-ribose) polymerase, catalytic domainPFAMPF00644PARPcoord: 602..805
e-value: 4.8E-45
score: 153.5
IPR012317Poly(ADP-ribose) polymerase, catalytic domainPROSITEPS51059PARP_CATALYTICcoord: 582..813
score: 63.278774
IPR036420BRCT domain superfamilyGENE3D3.40.50.10190BRCT domaincoord: 185..285
e-value: 4.0E-22
score: 80.1
IPR036420BRCT domain superfamilySUPERFAMILY52113BRCT domaincoord: 190..279
IPR036616Poly(ADP-ribose) polymerase, regulatory domain superfamilyGENE3D1.20.142.10coord: 450..591
e-value: 4.6E-19
score: 70.6
IPR036616Poly(ADP-ribose) polymerase, regulatory domain superfamilySUPERFAMILY47587Domain of poly(ADP-ribose) polymerasecoord: 454..590
NoneNo IPR availableGENE3D3.90.640.80coord: 60..180
e-value: 2.0E-25
score: 91.2
NoneNo IPR availableGENE3D3.90.228.10coord: 594..808
e-value: 5.0E-44
score: 152.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 171..193
NoneNo IPR availablePANTHERPTHR10459:SF107POLY [ADP-RIBOSE] POLYMERASEcoord: 4..808
NoneNo IPR availablePANTHERPTHR10459DNA LIGASEcoord: 4..808
NoneNo IPR availableCDDcd17747BRCT_PARP1coord: 192..267
e-value: 3.80545E-20
score: 83.3482
NoneNo IPR availableCDDcd01437parp_likecoord: 454..805
e-value: 1.64903E-106
score: 329.231
NoneNo IPR availableSUPERFAMILY56399ADP-ribosylationcoord: 592..808
IPR036930WGR domain superfamilySUPERFAMILY142921WGR domain-likecoord: 303..432

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0197.1MC02g0197.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006302 double-strand break repair
biological_process GO:0070212 protein poly-ADP-ribosylation
biological_process GO:0006471 protein ADP-ribosylation
cellular_component GO:0005730 nucleolus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003950 NAD+ ADP-ribosyltransferase activity
molecular_function GO:1990404 protein ADP-ribosylase activity