MC02g0172 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g0172 vs. ExPASy Swiss-Prot
Match: Q0WPH8 (Serine/threonine-protein kinase Nek5 OS=Arabidopsis thaliana OX=3702 GN=NEK5 PE=1 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 1.2e-263
Identity = 556/1001 (55.54%), Postives = 670/1001 (66.93%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYE+MEQIGRGAFGAAILV+HK+E+KKYVLKKIRLARQTERCRRSAHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNG++FPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSN+VLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCC+YEMAA+RPAFKAFDMAGLISK+NRSSIGPLP CYSPSLK LIK
Sbjct: 181 DIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPE+RPNASE+LKHPYLQP++EQYRP++S     TPEKP ++R+ RRSMAESQNS
Sbjct: 241 GMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLS-AASITPEKPLNSREGRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEE--QTHNICSL 360
           NSS S+KD+   S+KN    V +  +KVTE+D   VDD D  + +    E     ++ + 
Sbjct: 301 NSS-SEKDNFYVSDKNIRYVVPSNGNKVTETDSGFVDDEDILDHVQQSAENGNLQSVSAT 360

Query: 361 KVNDPMLTKPSQEDHQSNT-ESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQ 420
           K +   + KP   D + +  + R PKTIRNIM+ LKE K REN SP+R NR +      Q
Sbjct: 361 KPDGHGILKPVHSDQRPDVIQPRHPKTIRNIMMVLKEEKARENGSPMRSNRSRPSSVPTQ 420

Query: 421 KAHAEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPK 480
           K + E P K+ K       SK+NA TPI  P       S R       KH +PV+D +PK
Sbjct: 421 KNNVETPSKIPKLGDIAHSSKTNASTPI--PPSKLASDSARTPGSFPPKHHMPVIDSSPK 480

Query: 481 TKPRHDSSPPLYPVK-QVEDGLSAKSRQRTPPNLLRRPSFPA-RTRQVGLDVPDAVSSME 540
            KPR+D   P    K + E+ +S K RQRTPP L RR S  A ++RQ+G D+        
Sbjct: 481 LKPRNDRISPSPAAKHEAEEAMSVKRRQRTPPTLPRRTSLIAHQSRQLGADI-------S 540

Query: 541 NIRQKETSE--EGRTSNQVHSSHEDHVVSMELSKETQ-HNTSEHCSKGMQTDSSNSVSSS 600
           N+  KET++      S    +SH+  V +  +S   +   TS   +KGMQ++SSNS+SSS
Sbjct: 541 NMAAKETAKLHPSVPSESETNSHQSRVHASPVSTTPEPKRTSVGSAKGMQSESSNSISSS 600

Query: 601 VSIQGFEICDDAATPFSDITEPLF--DHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRD 660
           +S+Q FE+CDDA+TP+ D+TE     DH+   H E   + P         ++S   ++R 
Sbjct: 601 LSMQAFELCDDASTPYIDMTEHTTPDDHRRSCHSEYSYSFP---------DISSEMMIR- 660

Query: 661 KGNHGVDAKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINM 720
           +  H    + TE    +S     + NT A  + E                + KDD P  +
Sbjct: 661 RDEHSTSMRLTEIPDSVS----GVQNTIAHHQPEREQGSCPT--------VLKDDSPATL 720

Query: 721 PSNNRPNMVTETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTL-- 780
            S   PN     +     GDDKFTV+E +SSV     L      S       D  T++  
Sbjct: 721 QS-YEPNTSQHQH-----GDDKFTVKEFVSSVPGPAPLPLHVEPSHQVNSHSDNKTSVMS 780

Query: 781 HNPSAEKLAS-SHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKD 840
            N + EK  S SH     DDVIHVIRHSSFRVGS+QPV E++EVGVQNVD+GKL+ VV+D
Sbjct: 781 QNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSDQPVMESVEVGVQNVDMGKLINVVRD 840

Query: 841 ELELKNGTTTTPLTLKSSGYS-------------DAVCLKSNTSDHSSIKETSIGNPPSV 900
           E+E++ G T +      S  S             D +   S T   +S  ++S    P+ 
Sbjct: 841 EMEVRKGATPSESPTTRSIISEPDSRTEPRPKEPDPITNYSETKSFNSCSDSS----PAE 900

Query: 901 PKTNSDSSEHTTKPITPVVDEEVPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLE 960
            +TNS   E  T P  PV         KETLD+KSFRQRAEALEGLLELSA+LL+ +RLE
Sbjct: 901 TRTNSFVPEEETTPTPPV---------KETLDIKSFRQRAEALEGLLELSADLLEQSRLE 949

Query: 961 ELAVVLKPFGKDKVSPRETAIWLAKSLKGMMIED--NSRSS 974
           ELA+VL+PFGK+KVSPRETAIWLAKSLKGMMIED  N+ SS
Sbjct: 961 ELAIVLQPFGKNKVSPRETAIWLAKSLKGMMIEDINNNNSS 949

BLAST of MC02g0172 vs. ExPASy Swiss-Prot
Match: Q94CU5 (Serine/threonine-protein kinase Nek5 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK5 PE=2 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.1e-218
Identity = 493/988 (49.90%), Postives = 617/988 (62.45%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           M+SRMDQYE+MEQIGRGAFGAAILVNHK+EKKKYVLKKIRLARQTERCR+SAHQEMALIA
Sbjct: 1   MDSRMDQYEVMEQIGRGAFGAAILVNHKTEKKKYVLKKIRLARQTERCRKSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           R+QHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKK+NG +FPEEKL KWF QL LA
Sbjct: 61  RLQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKANGTYFPEEKLLKWFAQLALA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           V+YLHSNFVLHRDLKCSNIFLTKDQD+RLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VDYLHSNFVLHRDLKCSNIFLTKDQDIRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLP CYSPS+K+LIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPPCYSPSMKSLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRP--SISPTVDFTPEKPRSARDSRRSMAESQ 300
            MLRK+PEHRP ASE+LK PYLQP++ QYRP   IS  +    +   S+R S++SM+ SQ
Sbjct: 241 SMLRKSPEHRPTASEILKSPYLQPYVNQYRPFADISHPIHSLEKPITSSRSSQKSMSGSQ 300

Query: 301 NSNSSNSDKDSMVSSEKNT--PATVSNCDDKVTESDMASVDDHDGSEPLLPCEEQTHN-- 360
            S+ S SD DS+ SSE+NT  P+T SN      +++ A   DH   +     ++   N  
Sbjct: 301 CSSISGSDIDSIQSSERNTSGPSTSSN---NTIDTEGAEATDHVSVKNCSRSDDVKSNKE 360

Query: 361 ICSLKVNDPMLTKPSQEDHQSNTESRRPKTIRNIMIALK-EGKVRENYSPIRGNRIKSGV 420
               ++     +K    D +   E ++PK I+ I+  L+ E K+R+N SPIR +R+K   
Sbjct: 361 TVGPELERQDSSKSIHVDQRPRNEIKQPKIIKKILTTLREESKLRQNNSPIRASRVKLNS 420

Query: 421 ASNQKAHAEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVD 480
            SN++  ++      K    +  S  +++      AK   E  +R Q     KH  P+V+
Sbjct: 421 PSNREQLSDDS----KHSSDISSSSKSSEVTSRESAKVICEPVKRAQASPPLKHLSPIVE 480

Query: 481 PTPKTKPRHDSSPPLYPVKQVEDGLSA---KSRQRTPPNLLRRPSFPARTRQVGLDVPDA 540
            +PK K + D      P KQ  + + A   K + RTPP+  RR S P R R +G + P  
Sbjct: 481 HSPKAKIKQDEPLQPDPAKQAMEDVDAAVGKVKNRTPPSYSRRLSIPPR-RPLGAESP-L 540

Query: 541 VSSMENIRQKETSEEGRT-SNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSV 600
            +  +    K   E  ++    VH    D +                   G+Q    N+ 
Sbjct: 541 HADTKRAHNKVIKERAKSPCRPVHGPDNDIIEPPGFPMAPPSPLG-----GVQMKVGNAR 600

Query: 601 SSSVSIQGFEICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLR 660
           + S   +   I +D+    SD +     +   E+ E+ E      S  L S         
Sbjct: 601 AKSAPPRAVSIKEDS----SDCSSSTIAY--AENTELSEPSKQDSSAQLVSSCKCSIPDA 660

Query: 661 DKGNHGVDAKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPIN 720
               H + A P   S E+++ N            E+ + E    + SE+  IFKD++P +
Sbjct: 661 AIQKHDLTAMP---SSELNTTNFQKSMASNDDVCENLALEPSSDI-SEQVSIFKDNVPCS 720

Query: 721 MPSNNRPNMVTETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTLH 780
             S +  N + + +      +DKFTV+ELLSSVA+    V++ + +  K   P +     
Sbjct: 721 KISQSTANAIVQND------EDKFTVQELLSSVADIAPFVSTKNFALEKGSPPIQ----- 780

Query: 781 NPSAEKLASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDEL 840
             S E+ +S HL    +DVIHVIRHSSFRV  EQ V EN E+GVQ+ DVGKL+ VV++E+
Sbjct: 781 --SLERTSSPHLNPPIEDVIHVIRHSSFRVCGEQAVAENAEMGVQSSDVGKLLNVVREEV 840

Query: 841 ELKNGTTTTPLTLKSSGYSDAVCLKSNTSDHSSIKE----TSIGNPPSVPKTNSDSSEHT 900
           + ++  +     L      D    K N S+ ++I      + +    +VP+ NS     T
Sbjct: 841 DSRSIPSN---NLVPHRLPDCAAPKPNISETNTISSKTACSDVVKFLTVPEVNS-----T 900

Query: 901 TKPITPVVDEEVPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGK 960
           T  I     EE   T KE LDVKSFRQRAEALEGLLELSA+LLQHNRLEELAVVLKPFGK
Sbjct: 901 TTAINNGFKEEASPT-KEILDVKSFRQRAEALEGLLELSADLLQHNRLEELAVVLKPFGK 942

Query: 961 DKVSPRETAIWLAKSLKGMMIEDNSRSS 974
           DKVSPRETAIWLAKS KGMM ++ SRSS
Sbjct: 961 DKVSPRETAIWLAKSFKGMMNDEASRSS 942

BLAST of MC02g0172 vs. ExPASy Swiss-Prot
Match: Q60DG4 (Serine/threonine-protein kinase Nek4 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK4 PE=2 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 1.4e-200
Identity = 467/992 (47.08%), Postives = 604/992 (60.89%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQ+GRGAFGAAILVNHK E+KKYVLKKIRLARQTERCR+SAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQVGRGAFGAAILVNHKIERKKYVLKKIRLARQTERCRKSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           R+QHPYIVEFKEAWVEKGCYVCIVTGYCEGGDM ELMKK NG +FPEEKL KWF QL+LA
Sbjct: 61  RLQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMDELMKKLNGTYFPEEKLLKWFAQLVLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           V+YLHSN+VLHRDLKCSNIFLTKDQD+RLGDFGLAKTLK DDL SSVVGTPNYMCPELL 
Sbjct: 121 VDYLHSNYVLHRDLKCSNIFLTKDQDIRLGDFGLAKTLKEDDLTSSVVGTPNYMCPELLT 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLP+CYS S+KTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPACYSSSMKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRP-SISPTVDFTPEKPRS-ARDSRRSMAESQ 300
            MLRK+PEHRP ASE+LK+PYLQP++ Q RP S +PT    PEKP S +R ++R  +ESQ
Sbjct: 241 SMLRKSPEHRPTASEILKNPYLQPYVNQCRPLSDAPTPIRMPEKPLSTSRSNQRCTSESQ 300

Query: 301 NSNSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCE-----EQTH 360
           +S+ S SD DS  SS+++T     + D K+  +D+ S+ D D ++    C          
Sbjct: 301 SSSISCSDIDSTQSSDRSTSGGAPSTDSKL--NDIRSIQDADRADSDEKCVTPEDLRGNK 360

Query: 361 NICSLKVNDPMLTKPSQEDHQSNTESRRPKTIRNIMIALK-EGKVRENYSPIRGNRIKSG 420
           NI   ++     +K   + H+   ES++PK I  IM  L+ E ++REN SP+  + +K  
Sbjct: 361 NISGAELKRQDSSKSVHQHHRG--ESKQPKIIEKIMTTLREESRLRENNSPVSSSGVKLT 420

Query: 421 VASNQKAHAEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVV 480
            A + K  AE   +  +P   V  S    D         + E    +QV    K   P V
Sbjct: 421 SAVSNKNQAEQSSESSRPHSGVSYSSKFGDISSNGWTNTSDECVDPVQVPLQLKQLSPTV 480

Query: 481 DPTPKTKPRHDSSPPLYPVKQV-EDGLSAK--SRQRTPPNLLRRPSFPARTRQVGLD-VP 540
           +  PK K    S+P   P KQ+ E+G SA   S+ ++ P+  RRPS P R    G+  VP
Sbjct: 481 EHCPKLKNSGSSTPE--PAKQIAENGSSASGMSKTKSSPSSSRRPS-PQRQTVAGIPIVP 540

Query: 541 DAVSSMENIRQKETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNS 600
             VS   +I+ +      R ++  ++S  +    + +S     N SE   K   + +  +
Sbjct: 541 FTVSKRAHIKAESEKTPPRPAHSPNNSLHNLPPLIPIST----NLSEENIKLGNSQAMPA 600

Query: 601 VSSSVSIQGFEICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLL 660
               V+    E     +    D  E     ++ E +      PP   P L +        
Sbjct: 601 PLEFVTAASKEDISFYSNSVVDCVEKAEPSEVFESNSPAYLTPPWTGPVLDA-------- 660

Query: 661 RDKGNHGVDAKPTERSFEISSDNVNLHNTEAGVKKESPSAELD-LPLGSEERFIFKDDIP 720
             KG +G+ A P     EI +    L  + A     S S+ LD   L  E+ F+ KDD  
Sbjct: 661 --KGENGLIAIPCS---EIHTG--TLQKSMASNDDSSLSSPLDTFYLSFEQEFVCKDD-- 720

Query: 721 INMPSNNRPNMVTETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTT 780
                 ++ +    + +T   G+DKFTV+ELL+S       V SP  S+    LP+  ++
Sbjct: 721 ------SQSSKHGHSAVTLLSGEDKFTVQELLASTP-----VISPFVSSTSNTLPEDKSS 780

Query: 781 LHNPSAEKLASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKD 840
               S +K + SH     D     IR +SF V  E P +E ++   ++    KL+ VV++
Sbjct: 781 YQ--SFKKQSDSHSGPPVDVPAQTIRLNSFLVSDEWPTSETVQGEARDTAASKLLNVVRE 840

Query: 841 ELELKNGTTTTPLTLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTK 900
           + ++++ + +T  + + SG       ++      ++ ET++ +  S+P  +      T  
Sbjct: 841 DFDVRSSSCST--STQPSG-------QTPVRSKLNVPETNLASNISIPSISEAVRLSTAM 900

Query: 901 PITPVVDE------EVPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLK 960
            + P   E      E  + AKE LDV SFRQRAEALEGLLELSA+LL++NRLEELA+VL+
Sbjct: 901 DVKPYTSEASNGVKEEASPAKEALDVTSFRQRAEALEGLLELSADLLENNRLEELAIVLQ 942

Query: 961 PFGKDKVSPRETAIWLAKSLKGMMIEDNSRSS 974
           PFGK+KVSPRETAIWLA+S KGMM E+  R S
Sbjct: 961 PFGKNKVSPRETAIWLARSFKGMMNEEGGRLS 942

BLAST of MC02g0172 vs. ExPASy Swiss-Prot
Match: Q6ZEZ5 (Serine/threonine-protein kinase Nek3 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK3 PE=1 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 6.2e-116
Identity = 198/311 (63.67%), Postives = 256/311 (82.32%), Query Frame = 0

Query: 5   MDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+QYE++EQIG+G+FG+A+LV HK EKK+YVLKKIRLARQT+RCRRSAHQEM LIA+V++
Sbjct: 1   MEQYEVLEQIGKGSFGSALLVRHKVEKKRYVLKKIRLARQTDRCRRSAHQEMELIAKVRN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           PYIVE+K++WVEKGCYVCIV GYCEGGDM+E +KK+N  +F EE+LC W  QLL+A++YL
Sbjct: 61  PYIVEYKDSWVEKGCYVCIVIGYCEGGDMSEAIKKANSNYFSEERLCMWLVQLLMALDYL 120

Query: 125 HSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H N +LHRD+KCSNIFLTKDQ++RLGDFGLAK L +DDL SSVVGTP+YMCPELLADIPY
Sbjct: 121 HVNHILHRDVKCSNIFLTKDQNIRLGDFGLAKVLTSDDLTSSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEM A +PAFKAFDM  LI+KI++S + PLP+ YS + + LIK MLR
Sbjct: 181 GSKSDIWSLGCCLYEMTALKPAFKAFDMQTLINKISKSVLAPLPTIYSGAFRGLIKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNSNSSN 304
           K+P+HRP+A+ELLKHP+LQPF+ + +   SP  +  P+  +++     +  +++ S S +
Sbjct: 241 KSPDHRPSAAELLKHPHLQPFVLELQLKSSPARNLFPDTNKASCSDDENNWKAKYSKSHS 300

Query: 305 SDKDSMVSSEK 316
              D +V  +K
Sbjct: 301 FKVDRIVKVDK 311

HSP 2 Score: 82.4 bits (202), Expect = 3.1e-14
Identity = 56/132 (42.42%), Postives = 70/132 (53.03%), Query Frame = 0

Query: 837 TTPLTLKSSGYSDAVCLKSNTS-----DHSSIKETSIGNPPSVPKTNSDSSEHTTKPITP 896
           TT L L     +D    K   +       SS   T   + PS+  T+S+          P
Sbjct: 461 TTKLKLYPPSITDQSITKDKCTFQVLRSDSSKNHTGDSSDPSILGTDSN----------P 520

Query: 897 VVDEEVPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPR 956
           ++        +   D  S+RQRAEALEGLLE SA+LLQ  R EEL ++LKPFG  K SPR
Sbjct: 521 LITSSSDWMKQRRFDTTSYRQRAEALEGLLEFSAQLLQQERFEELGILLKPFGPGKASPR 580

Query: 957 ETAIWLAKSLKG 964
           ETAIWL+KS KG
Sbjct: 581 ETAIWLSKSFKG 582

BLAST of MC02g0172 vs. ExPASy Swiss-Prot
Match: Q10GB1 (Serine/threonine-protein kinase Nek1 OS=Oryza sativa subsp. japonica OX=39947 GN=NEK1 PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.1e-115
Identity = 208/335 (62.09%), Postives = 262/335 (78.21%), Query Frame = 0

Query: 5   MDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+QYE++EQIG+GAFG+A+LV HK EKKKYVLKKIRLARQT+R RRSAHQEM LIA V++
Sbjct: 1   MEQYEVLEQIGKGAFGSALLVRHKVEKKKYVLKKIRLARQTDRTRRSAHQEMQLIATVRN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCI+ GYCEGGDMAE +K++ G HF EEKLCKW  QLL+A++YL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCEGGDMAEAIKRATGDHFSEEKLCKWLVQLLMALDYL 120

Query: 125 HSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H+N +LHRD+KCSNIFLT+DQ +RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFLTRDQSIRLGDFGLAKILTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEM A RPAFKAFDM  LISKI +S + PLP+ YS + + LIK MLR
Sbjct: 181 GTKSDIWSLGCCIYEMTALRPAFKAFDMQALISKITKSIVSPLPTRYSGAFRGLIKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNSNSSN 304
           K+PEHRP+A+ELLKHP+LQP++ Q     SP  +  P   +S  D  + M     S   +
Sbjct: 241 KSPEHRPSAAELLKHPHLQPYVLQVHLKSSPARNIIPSH-QSPIDKVKKMTFPTESMCRS 300

Query: 305 SDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDH 340
             + + + +E+    +  + + K T S + S+ D+
Sbjct: 301 KGRRNSLGNERIVTFSKPSPERKFT-SSIQSIKDY 333

HSP 2 Score: 84.7 bits (208), Expect = 6.2e-15
Identity = 42/64 (65.62%), Postives = 50/64 (78.12%), Query Frame = 0

Query: 899 ATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLA 958
           +T +   D  S++QRAEALEGLLE SA+LLQ  R +EL V+LKPFG +KVSPRETAIWL 
Sbjct: 532 STRQRRFDTSSYQQRAEALEGLLEFSAQLLQQERYDELGVLLKPFGPEKVSPRETAIWLT 591

Query: 959 KSLK 963
           KS K
Sbjct: 592 KSFK 595

BLAST of MC02g0172 vs. NCBI nr
Match: XP_022153020.1 (serine/threonine-protein kinase Nek5 [Momordica charantia] >XP_022153021.1 serine/threonine-protein kinase Nek5 [Momordica charantia])

HSP 1 Score: 1890 bits (4895), Expect = 0.0
Identity = 972/973 (99.90%), Postives = 972/973 (99.90%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEEQTHNICSLKV 360
           NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDG EPLLPCEEQTHNICSLKV
Sbjct: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGXEPLLPCEEQTHNICSLKV 360

Query: 361 NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH 420
           NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH
Sbjct: 361 NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH 420

Query: 421 AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP 480
           AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP
Sbjct: 421 AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP 480

Query: 481 RHDSSPPLYPVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGLDVPDAVSSMENIRQK 540
           RHDSSPPLYPVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGLDVPDAVSSMENIRQK
Sbjct: 481 RHDSSPPLYPVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGLDVPDAVSSMENIRQK 540

Query: 541 ETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGFEI 600
           ETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGFEI
Sbjct: 541 ETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGFEI 600

Query: 601 CDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVDAKP 660
           CDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVDAKP
Sbjct: 601 CDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVDAKP 660

Query: 661 TERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPNMVT 720
           TERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPNMVT
Sbjct: 661 TERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPNMVT 720

Query: 721 ETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTLHNPSAEKLASSH 780
           ETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTLHNPSAEKLASSH
Sbjct: 721 ETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTLHNPSAEKLASSH 780

Query: 781 LPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTTTTPL 840
           LPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTTTTPL
Sbjct: 781 LPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTTTTPL 840

Query: 841 TLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEEVPAT 900
           TLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEEVPAT
Sbjct: 841 TLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEEVPAT 900

Query: 901 AKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLAKS 960
           AKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLAKS
Sbjct: 901 AKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLAKS 960

Query: 961 LKGMMIEDNSRSS 973
           LKGMMIEDNSRSS
Sbjct: 961 LKGMMIEDNSRSS 973

BLAST of MC02g0172 vs. NCBI nr
Match: XP_008449590.1 (PREDICTED: serine/threonine-protein kinase Nek5 isoform X1 [Cucumis melo])

HSP 1 Score: 1636 bits (4237), Expect = 0.0
Identity = 855/976 (87.60%), Postives = 911/976 (93.34%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQP++EQYRPSISPTVDF+P+KPR  RDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPTVDFSPDKPRLTRDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEE-QTHNICSLK 360
           NSSNSD+DS+ SS+KNTPA  +NCDDKVT+SDMASVDDH+GSEPLLPCEE QTHN+CSLK
Sbjct: 301 NSSNSDRDSLFSSDKNTPAMTANCDDKVTDSDMASVDDHEGSEPLLPCEEEQTHNVCSLK 360

Query: 361 VNDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKA 420
           VNDPM +KPSQED QSN+ESRRPKTIRNIM+ALKEGK RE+YSP+RGNR+KSGVASNQKA
Sbjct: 361 VNDPMHSKPSQED-QSNSESRRPKTIRNIMLALKEGKARESYSPVRGNRVKSGVASNQKA 420

Query: 421 HAEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTK 480
           +AEVPPKVIKPP+AVPGSKS+ DTPITV AKANIESSRR QVV+T KHQLPVVDPTPK+K
Sbjct: 421 YAEVPPKVIKPPMAVPGSKSSPDTPITVSAKANIESSRRNQVVSTPKHQLPVVDPTPKSK 480

Query: 481 PRHDSSPPLY-PVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGL-DVPDAVSSMENI 540
           PRHDSSPPL  PVK VEDG+S+KSRQRTPP+LLRRPSFPARTRQVG  DVPDAVSS+E I
Sbjct: 481 PRHDSSPPLCRPVKPVEDGVSSKSRQRTPPSLLRRPSFPARTRQVGQQDVPDAVSSVERI 540

Query: 541 RQKETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQG 600
           RQKET  E RTS++VHSSH+DHVVS+EL+KETQH +SE CSKGMQTDSSNSVSSSVSIQG
Sbjct: 541 RQKETLPEERTSHKVHSSHQDHVVSVELTKETQHISSEPCSKGMQTDSSNSVSSSVSIQG 600

Query: 601 FEICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVD 660
           FEICDDAATPF+DI EP+FDHQ +EHDEIEE HPP CSP LQ EMSEH LL DKGNHGV 
Sbjct: 601 FEICDDAATPFADIAEPVFDHQSIEHDEIEEIHPPVCSPSLQYEMSEHNLLEDKGNHGVY 660

Query: 661 AKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPN 720
            KPT+ SFEISSD VNLH+TE G+KKESPS ELDLPL SE RF+FKDDIPINMP+N   N
Sbjct: 661 DKPTKCSFEISSDIVNLHDTEVGIKKESPSVELDLPLRSEGRFVFKDDIPINMPTNGS-N 720

Query: 721 MVTETNITSSFGDDKFTVRELLSSVAE-STALVASPSTSTHKCLLPDKVTTLHNPSAEKL 780
           MV+E    SS GDDK+TVRELLSSVAE +T LVASPS+STHKCLLPDKVTTLH  S EKL
Sbjct: 721 MVSEKITISSHGDDKYTVRELLSSVAEPATPLVASPSSSTHKCLLPDKVTTLHTSSPEKL 780

Query: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840
           ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT
Sbjct: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840

Query: 841 TTPLTLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEE 900
            TPLTLKS+  SDA+CLK NT +HS+IKE++  NPP +P TNSDS+EHT KP+TPVV+EE
Sbjct: 841 NTPLTLKSNSCSDALCLKPNTCEHSAIKESTGANPPMLPTTNSDSTEHT-KPVTPVVEEE 900

Query: 901 VPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960
           VPA  KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW
Sbjct: 901 VPA--KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960

Query: 961 LAKSLKGMMIEDNSRS 972
           LAKSLKGMMIEDN+RS
Sbjct: 961 LAKSLKGMMIEDNARS 971

BLAST of MC02g0172 vs. NCBI nr
Match: XP_038901200.1 (serine/threonine-protein kinase Nek5 [Benincasa hispida])

HSP 1 Score: 1634 bits (4231), Expect = 0.0
Identity = 860/975 (88.21%), Postives = 906/975 (92.92%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQP++EQYRPSISPTVDF+P+KPR ARDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPTVDFSPDKPRPARDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEEQTHNICSLKV 360
           NSSNSD+DS+ SS+KNTPAT SNCDDKVT+SDMASVDDHDGSEPL P EEQTHN+CSLKV
Sbjct: 301 NSSNSDRDSLFSSDKNTPATASNCDDKVTDSDMASVDDHDGSEPLHPSEEQTHNMCSLKV 360

Query: 361 NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH 420
           NDP+ +KPSQED QSNTESRRPKTIRNIM+ALKEGK RENYSP+RGNR+KSGVASNQKA+
Sbjct: 361 NDPVHSKPSQED-QSNTESRRPKTIRNIMLALKEGKARENYSPVRGNRVKSGVASNQKAY 420

Query: 421 AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP 480
           AE+PPKVIK P+AVPG+KSN DTPITV  KANIESSRR QVVAT KHQLPV DPTPKTKP
Sbjct: 421 AEMPPKVIKLPMAVPGAKSNPDTPITVSTKANIESSRRNQVVATPKHQLPVADPTPKTKP 480

Query: 481 RHDSSPPLY-PVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGLDVPDAVSSMENIRQ 540
           RHDSSPPL  PVK +EDGLS+KSRQRTPP LLRRPSFPARTRQVG DVPDAVSS E I Q
Sbjct: 481 RHDSSPPLCCPVKPMEDGLSSKSRQRTPPTLLRRPSFPARTRQVGQDVPDAVSSTERIHQ 540

Query: 541 KETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGFE 600
           KETS E RTSN+VHSS++DHVVS+EL+KETQH +SEHCSKGMQTDSSNSVSSSVSIQGFE
Sbjct: 541 KETSPEERTSNKVHSSNQDHVVSVELTKETQHTSSEHCSKGMQTDSSNSVSSSVSIQGFE 600

Query: 601 ICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVDAK 660
           I DDAATPF+ I EP+FDHQ +EHDEIEETHPP CS  LQ EMSE  LL  KGNH V  K
Sbjct: 601 IRDDAATPFAYIAEPVFDHQSIEHDEIEETHPPVCSSSLQYEMSEQNLLGVKGNHEVYDK 660

Query: 661 PTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPNMV 720
           P E SFEISSD VNLHNTEAGV+KESPS ELDLPL SE RFIFKDD+PINM SN RPNMV
Sbjct: 661 PAECSFEISSDIVNLHNTEAGVEKESPSVELDLPLKSEGRFIFKDDVPINMASN-RPNMV 720

Query: 721 TETNITSSFGDDKFTVRELLSSVAE-STALVASPSTSTHKCLLPDKVTTLHNPSAEKLAS 780
           +E    SS GDDKFTVRELLSSVAE +T LVA+PS++THKCLLPDKVTTLHN SAEKLA+
Sbjct: 721 SEPFTISSHGDDKFTVRELLSSVAEPTTPLVATPSSTTHKCLLPDKVTTLHNSSAEKLAA 780

Query: 781 SHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTTTT 840
           SHLPAAFDD+IHVIRHSSFRVGSEQPVTENLEVGVQ+VDVGKLVTVVKDELELKNGTTT 
Sbjct: 781 SHLPAAFDDIIHVIRHSSFRVGSEQPVTENLEVGVQSVDVGKLVTVVKDELELKNGTTT- 840

Query: 841 PLTLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEEVP 900
           PLTLKS+  SDA+CLK NTSDHS +KE+S GNPP VP TNSDSSEHT K ITPVV+EEVP
Sbjct: 841 PLTLKSNSCSDALCLKPNTSDHSGVKESSGGNPPMVPTTNSDSSEHT-KLITPVVEEEVP 900

Query: 901 ATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLA 960
           A  KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLA
Sbjct: 901 A--KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLA 960

Query: 961 KSLKGMMIEDNSRSS 973
           KSLKGMMIEDN+RSS
Sbjct: 961 KSLKGMMIEDNARSS 969

BLAST of MC02g0172 vs. NCBI nr
Match: KAA0061732.1 (serine/threonine-protein kinase Nek5 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1631 bits (4223), Expect = 0.0
Identity = 852/976 (87.30%), Postives = 908/976 (93.03%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQP++EQYRPSISPTVDF+P+KPR  RDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPTVDFSPDKPRLTRDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEE-QTHNICSLK 360
           NSSNSD+DS+ SS+KNTPAT +NCDDKVT+SDMAS+DDH+GSEPLLPCEE QTHN+CSLK
Sbjct: 301 NSSNSDRDSLFSSDKNTPATTANCDDKVTDSDMASIDDHEGSEPLLPCEEEQTHNVCSLK 360

Query: 361 VNDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKA 420
           VNDPM +KPSQED QSN+ESRRPKTIRNIM+ALKEGK RE+YSP+RGNR+KSGVASNQKA
Sbjct: 361 VNDPMHSKPSQED-QSNSESRRPKTIRNIMLALKEGKARESYSPVRGNRVKSGVASNQKA 420

Query: 421 HAEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTK 480
           +A+VPPKVIKPP+AVPGSK + DTPITV AKANIESSRR QVV T KHQLPVVDPTPKTK
Sbjct: 421 YADVPPKVIKPPMAVPGSKYSPDTPITVSAKANIESSRRNQVVCTPKHQLPVVDPTPKTK 480

Query: 481 PRHDSSPPLY-PVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGL-DVPDAVSSMENI 540
           PRHDSSPPL  PVK VEDG+S+KSRQRTPP+LLRRPSFPARTRQVG  DVPDAVSS+E I
Sbjct: 481 PRHDSSPPLCRPVKPVEDGVSSKSRQRTPPSLLRRPSFPARTRQVGQQDVPDAVSSVERI 540

Query: 541 RQKETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQG 600
           RQKET  E RT ++VHSSH+DHVVS+EL+KETQH +SE CSKGMQTDSSNSVSSSVSIQG
Sbjct: 541 RQKETLPEERTCHKVHSSHQDHVVSVELTKETQHISSEPCSKGMQTDSSNSVSSSVSIQG 600

Query: 601 FEICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVD 660
           FEICDDAATPF+DI EP+FDHQ +EHDEIEE HPP CSP LQ EMSEH LL DKGNHGV 
Sbjct: 601 FEICDDAATPFADIAEPVFDHQSIEHDEIEEIHPPVCSPSLQYEMSEHNLLEDKGNHGVY 660

Query: 661 AKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPN 720
            KPT+ SFEISSD VNLH+TE G+KKESPS ELDLPL SE RF+FKDDIPINMP+N   N
Sbjct: 661 DKPTKCSFEISSDIVNLHDTEVGIKKESPSVELDLPLRSEGRFVFKDDIPINMPTNGS-N 720

Query: 721 MVTETNITSSFGDDKFTVRELLSSVAE-STALVASPSTSTHKCLLPDKVTTLHNPSAEKL 780
           MV+E    SS GDDK+TVRELLSSVAE +T LVASPS+STHKCLLPDKVTTLH  S EKL
Sbjct: 721 MVSEQITISSHGDDKYTVRELLSSVAEPATPLVASPSSSTHKCLLPDKVTTLHTSSPEKL 780

Query: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840
           ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT
Sbjct: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840

Query: 841 TTPLTLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEE 900
            T LTLKS+  SDA+CLK NT +HS+IKE++  NPP +P TNSDS+EHT KP+TPVV+EE
Sbjct: 841 NTSLTLKSNSCSDALCLKPNTCEHSAIKESTGANPPMLPTTNSDSTEHT-KPVTPVVEEE 900

Query: 901 VPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960
           VPA  KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW
Sbjct: 901 VPA--KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960

Query: 961 LAKSLKGMMIEDNSRS 972
           LAKSLKGMMIEDN+RS
Sbjct: 961 LAKSLKGMMIEDNARS 971

BLAST of MC02g0172 vs. NCBI nr
Match: XP_011657591.1 (serine/threonine-protein kinase Nek5 [Cucumis sativus] >KGN48049.1 hypothetical protein Csa_003049 [Cucumis sativus])

HSP 1 Score: 1629 bits (4219), Expect = 0.0
Identity = 851/977 (87.10%), Postives = 904/977 (92.53%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQP++EQYRPSISP VDF+P+KPR  RDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPNVDFSPDKPRLTRDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEEQTHNICSLKV 360
           NSSNSD+DS+ SS+KNTPA  +NCD+KVT+SDMASVDDH+GSEPLLPCEEQTHN+CSLKV
Sbjct: 301 NSSNSDRDSLFSSDKNTPAMTANCDEKVTDSDMASVDDHEGSEPLLPCEEQTHNVCSLKV 360

Query: 361 NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH 420
           NDPM  KPSQED QSN+ESRRPKTIRNIM+ALKEGK RENYSP+RGNR KSGVASNQKA 
Sbjct: 361 NDPMHPKPSQED-QSNSESRRPKTIRNIMLALKEGKARENYSPVRGNRAKSGVASNQKAF 420

Query: 421 AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP 480
           A+VPPKVIKPP+AVPGSKS+ DTPITV  KANIESSRR Q V+T KHQLPVVDPTPKT+P
Sbjct: 421 ADVPPKVIKPPMAVPGSKSSPDTPITVSTKANIESSRRNQAVSTPKHQLPVVDPTPKTRP 480

Query: 481 RHDSSPPLY-PVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGL-DVPDAVSSMENIR 540
           RHDSSPPL  PVK VEDG+S+KSRQ+TPP+LLRRPSFP RTRQVG  DVPDAVSS E IR
Sbjct: 481 RHDSSPPLCRPVKPVEDGVSSKSRQKTPPSLLRRPSFPVRTRQVGQQDVPDAVSSTERIR 540

Query: 541 QKETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGF 600
           QKET  E RT ++VHSSH+DHVVS+EL+KETQH +SEHCSKGMQTDSSNSVSSSVSIQGF
Sbjct: 541 QKETLPEERTCHKVHSSHQDHVVSVELTKETQHTSSEHCSKGMQTDSSNSVSSSVSIQGF 600

Query: 601 EICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGV-D 660
           EICDDAATPF+DI EP+FDHQ +EHDEIEE HPP CSP LQ E+SEH LL DKGNHGV D
Sbjct: 601 EICDDAATPFADIAEPVFDHQNIEHDEIEEIHPPVCSPSLQYEISEHNLLEDKGNHGVYD 660

Query: 661 AKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPN 720
              T+ SFE SSDNVNLHNTEAG+KKESPS ELD PL SE RFIFKDDIPI+MP+N   N
Sbjct: 661 KSTTKCSFETSSDNVNLHNTEAGIKKESPSVELDHPLRSEGRFIFKDDIPISMPTNGS-N 720

Query: 721 MVTETNITSSFGDDKFTVRELLSSVAE-STALVASPSTSTHKCLLPDKVTTLHNPSAEKL 780
           MV+E    SS GDDKFTVRELLSSVAE +T LVASPS+STHKCLLPDKVTTLH  SAEKL
Sbjct: 721 MVSEQITISSHGDDKFTVRELLSSVAEPATPLVASPSSSTHKCLLPDKVTTLHTSSAEKL 780

Query: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840
           A SHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT
Sbjct: 781 AGSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840

Query: 841 TTPLTLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEE 900
           TTPLTLKSS  SDA+C K NTS+HS+IKE++  NPP +P TNSDS+EHT KP+TPVV+EE
Sbjct: 841 TTPLTLKSSSCSDALCSKPNTSEHSAIKESTGANPPMLPTTNSDSTEHT-KPVTPVVEEE 900

Query: 901 VPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960
           VPA  KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW
Sbjct: 901 VPA--KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960

Query: 961 LAKSLKGMMIEDNSRSS 973
           LA+SLKGMMIEDN+RSS
Sbjct: 961 LARSLKGMMIEDNARSS 972

BLAST of MC02g0172 vs. ExPASy TrEMBL
Match: A0A6J1DJG0 (serine/threonine-protein kinase Nek5 OS=Momordica charantia OX=3673 GN=LOC111020624 PE=4 SV=1)

HSP 1 Score: 1890 bits (4895), Expect = 0.0
Identity = 972/973 (99.90%), Postives = 972/973 (99.90%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEEQTHNICSLKV 360
           NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDG EPLLPCEEQTHNICSLKV
Sbjct: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGXEPLLPCEEQTHNICSLKV 360

Query: 361 NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH 420
           NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH
Sbjct: 361 NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH 420

Query: 421 AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP 480
           AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP
Sbjct: 421 AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP 480

Query: 481 RHDSSPPLYPVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGLDVPDAVSSMENIRQK 540
           RHDSSPPLYPVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGLDVPDAVSSMENIRQK
Sbjct: 481 RHDSSPPLYPVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGLDVPDAVSSMENIRQK 540

Query: 541 ETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGFEI 600
           ETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGFEI
Sbjct: 541 ETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGFEI 600

Query: 601 CDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVDAKP 660
           CDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVDAKP
Sbjct: 601 CDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVDAKP 660

Query: 661 TERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPNMVT 720
           TERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPNMVT
Sbjct: 661 TERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPNMVT 720

Query: 721 ETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTLHNPSAEKLASSH 780
           ETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTLHNPSAEKLASSH
Sbjct: 721 ETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTLHNPSAEKLASSH 780

Query: 781 LPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTTTTPL 840
           LPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTTTTPL
Sbjct: 781 LPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTTTTPL 840

Query: 841 TLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEEVPAT 900
           TLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEEVPAT
Sbjct: 841 TLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEEVPAT 900

Query: 901 AKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLAKS 960
           AKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLAKS
Sbjct: 901 AKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLAKS 960

Query: 961 LKGMMIEDNSRSS 973
           LKGMMIEDNSRSS
Sbjct: 961 LKGMMIEDNSRSS 973

BLAST of MC02g0172 vs. ExPASy TrEMBL
Match: A0A1S3BME0 (serine/threonine-protein kinase Nek5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491429 PE=4 SV=1)

HSP 1 Score: 1636 bits (4237), Expect = 0.0
Identity = 855/976 (87.60%), Postives = 911/976 (93.34%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQP++EQYRPSISPTVDF+P+KPR  RDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPTVDFSPDKPRLTRDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEE-QTHNICSLK 360
           NSSNSD+DS+ SS+KNTPA  +NCDDKVT+SDMASVDDH+GSEPLLPCEE QTHN+CSLK
Sbjct: 301 NSSNSDRDSLFSSDKNTPAMTANCDDKVTDSDMASVDDHEGSEPLLPCEEEQTHNVCSLK 360

Query: 361 VNDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKA 420
           VNDPM +KPSQED QSN+ESRRPKTIRNIM+ALKEGK RE+YSP+RGNR+KSGVASNQKA
Sbjct: 361 VNDPMHSKPSQED-QSNSESRRPKTIRNIMLALKEGKARESYSPVRGNRVKSGVASNQKA 420

Query: 421 HAEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTK 480
           +AEVPPKVIKPP+AVPGSKS+ DTPITV AKANIESSRR QVV+T KHQLPVVDPTPK+K
Sbjct: 421 YAEVPPKVIKPPMAVPGSKSSPDTPITVSAKANIESSRRNQVVSTPKHQLPVVDPTPKSK 480

Query: 481 PRHDSSPPLY-PVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGL-DVPDAVSSMENI 540
           PRHDSSPPL  PVK VEDG+S+KSRQRTPP+LLRRPSFPARTRQVG  DVPDAVSS+E I
Sbjct: 481 PRHDSSPPLCRPVKPVEDGVSSKSRQRTPPSLLRRPSFPARTRQVGQQDVPDAVSSVERI 540

Query: 541 RQKETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQG 600
           RQKET  E RTS++VHSSH+DHVVS+EL+KETQH +SE CSKGMQTDSSNSVSSSVSIQG
Sbjct: 541 RQKETLPEERTSHKVHSSHQDHVVSVELTKETQHISSEPCSKGMQTDSSNSVSSSVSIQG 600

Query: 601 FEICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVD 660
           FEICDDAATPF+DI EP+FDHQ +EHDEIEE HPP CSP LQ EMSEH LL DKGNHGV 
Sbjct: 601 FEICDDAATPFADIAEPVFDHQSIEHDEIEEIHPPVCSPSLQYEMSEHNLLEDKGNHGVY 660

Query: 661 AKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPN 720
            KPT+ SFEISSD VNLH+TE G+KKESPS ELDLPL SE RF+FKDDIPINMP+N   N
Sbjct: 661 DKPTKCSFEISSDIVNLHDTEVGIKKESPSVELDLPLRSEGRFVFKDDIPINMPTNGS-N 720

Query: 721 MVTETNITSSFGDDKFTVRELLSSVAE-STALVASPSTSTHKCLLPDKVTTLHNPSAEKL 780
           MV+E    SS GDDK+TVRELLSSVAE +T LVASPS+STHKCLLPDKVTTLH  S EKL
Sbjct: 721 MVSEKITISSHGDDKYTVRELLSSVAEPATPLVASPSSSTHKCLLPDKVTTLHTSSPEKL 780

Query: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840
           ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT
Sbjct: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840

Query: 841 TTPLTLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEE 900
            TPLTLKS+  SDA+CLK NT +HS+IKE++  NPP +P TNSDS+EHT KP+TPVV+EE
Sbjct: 841 NTPLTLKSNSCSDALCLKPNTCEHSAIKESTGANPPMLPTTNSDSTEHT-KPVTPVVEEE 900

Query: 901 VPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960
           VPA  KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW
Sbjct: 901 VPA--KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960

Query: 961 LAKSLKGMMIEDNSRS 972
           LAKSLKGMMIEDN+RS
Sbjct: 961 LAKSLKGMMIEDNARS 971

BLAST of MC02g0172 vs. ExPASy TrEMBL
Match: A0A5A7V0M3 (Serine/threonine-protein kinase Nek5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold212G001070 PE=4 SV=1)

HSP 1 Score: 1631 bits (4223), Expect = 0.0
Identity = 852/976 (87.30%), Postives = 908/976 (93.03%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQP++EQYRPSISPTVDF+P+KPR  RDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPTVDFSPDKPRLTRDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEE-QTHNICSLK 360
           NSSNSD+DS+ SS+KNTPAT +NCDDKVT+SDMAS+DDH+GSEPLLPCEE QTHN+CSLK
Sbjct: 301 NSSNSDRDSLFSSDKNTPATTANCDDKVTDSDMASIDDHEGSEPLLPCEEEQTHNVCSLK 360

Query: 361 VNDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKA 420
           VNDPM +KPSQED QSN+ESRRPKTIRNIM+ALKEGK RE+YSP+RGNR+KSGVASNQKA
Sbjct: 361 VNDPMHSKPSQED-QSNSESRRPKTIRNIMLALKEGKARESYSPVRGNRVKSGVASNQKA 420

Query: 421 HAEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTK 480
           +A+VPPKVIKPP+AVPGSK + DTPITV AKANIESSRR QVV T KHQLPVVDPTPKTK
Sbjct: 421 YADVPPKVIKPPMAVPGSKYSPDTPITVSAKANIESSRRNQVVCTPKHQLPVVDPTPKTK 480

Query: 481 PRHDSSPPLY-PVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGL-DVPDAVSSMENI 540
           PRHDSSPPL  PVK VEDG+S+KSRQRTPP+LLRRPSFPARTRQVG  DVPDAVSS+E I
Sbjct: 481 PRHDSSPPLCRPVKPVEDGVSSKSRQRTPPSLLRRPSFPARTRQVGQQDVPDAVSSVERI 540

Query: 541 RQKETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQG 600
           RQKET  E RT ++VHSSH+DHVVS+EL+KETQH +SE CSKGMQTDSSNSVSSSVSIQG
Sbjct: 541 RQKETLPEERTCHKVHSSHQDHVVSVELTKETQHISSEPCSKGMQTDSSNSVSSSVSIQG 600

Query: 601 FEICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGVD 660
           FEICDDAATPF+DI EP+FDHQ +EHDEIEE HPP CSP LQ EMSEH LL DKGNHGV 
Sbjct: 601 FEICDDAATPFADIAEPVFDHQSIEHDEIEEIHPPVCSPSLQYEMSEHNLLEDKGNHGVY 660

Query: 661 AKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPN 720
            KPT+ SFEISSD VNLH+TE G+KKESPS ELDLPL SE RF+FKDDIPINMP+N   N
Sbjct: 661 DKPTKCSFEISSDIVNLHDTEVGIKKESPSVELDLPLRSEGRFVFKDDIPINMPTNGS-N 720

Query: 721 MVTETNITSSFGDDKFTVRELLSSVAE-STALVASPSTSTHKCLLPDKVTTLHNPSAEKL 780
           MV+E    SS GDDK+TVRELLSSVAE +T LVASPS+STHKCLLPDKVTTLH  S EKL
Sbjct: 721 MVSEQITISSHGDDKYTVRELLSSVAEPATPLVASPSSSTHKCLLPDKVTTLHTSSPEKL 780

Query: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840
           ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT
Sbjct: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840

Query: 841 TTPLTLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEE 900
            T LTLKS+  SDA+CLK NT +HS+IKE++  NPP +P TNSDS+EHT KP+TPVV+EE
Sbjct: 841 NTSLTLKSNSCSDALCLKPNTCEHSAIKESTGANPPMLPTTNSDSTEHT-KPVTPVVEEE 900

Query: 901 VPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960
           VPA  KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW
Sbjct: 901 VPA--KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960

Query: 961 LAKSLKGMMIEDNSRS 972
           LAKSLKGMMIEDN+RS
Sbjct: 961 LAKSLKGMMIEDNARS 971

BLAST of MC02g0172 vs. ExPASy TrEMBL
Match: A0A0A0KGX5 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G425860 PE=4 SV=1)

HSP 1 Score: 1629 bits (4219), Expect = 0.0
Identity = 851/977 (87.10%), Postives = 904/977 (92.53%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSNFVLHRDLKCSNIFLTKD+DVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNFVLHRDLKCSNIFLTKDKDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK
Sbjct: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPEHRPNASELLKHPYLQP++EQYRPSISP VDF+P+KPR  RDSRRSMAESQNS
Sbjct: 241 GMLRKNPEHRPNASELLKHPYLQPYVEQYRPSISPNVDFSPDKPRLTRDSRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEEQTHNICSLKV 360
           NSSNSD+DS+ SS+KNTPA  +NCD+KVT+SDMASVDDH+GSEPLLPCEEQTHN+CSLKV
Sbjct: 301 NSSNSDRDSLFSSDKNTPAMTANCDEKVTDSDMASVDDHEGSEPLLPCEEQTHNVCSLKV 360

Query: 361 NDPMLTKPSQEDHQSNTESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQKAH 420
           NDPM  KPSQED QSN+ESRRPKTIRNIM+ALKEGK RENYSP+RGNR KSGVASNQKA 
Sbjct: 361 NDPMHPKPSQED-QSNSESRRPKTIRNIMLALKEGKARENYSPVRGNRAKSGVASNQKAF 420

Query: 421 AEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPKTKP 480
           A+VPPKVIKPP+AVPGSKS+ DTPITV  KANIESSRR Q V+T KHQLPVVDPTPKT+P
Sbjct: 421 ADVPPKVIKPPMAVPGSKSSPDTPITVSTKANIESSRRNQAVSTPKHQLPVVDPTPKTRP 480

Query: 481 RHDSSPPLY-PVKQVEDGLSAKSRQRTPPNLLRRPSFPARTRQVGL-DVPDAVSSMENIR 540
           RHDSSPPL  PVK VEDG+S+KSRQ+TPP+LLRRPSFP RTRQVG  DVPDAVSS E IR
Sbjct: 481 RHDSSPPLCRPVKPVEDGVSSKSRQKTPPSLLRRPSFPVRTRQVGQQDVPDAVSSTERIR 540

Query: 541 QKETSEEGRTSNQVHSSHEDHVVSMELSKETQHNTSEHCSKGMQTDSSNSVSSSVSIQGF 600
           QKET  E RT ++VHSSH+DHVVS+EL+KETQH +SEHCSKGMQTDSSNSVSSSVSIQGF
Sbjct: 541 QKETLPEERTCHKVHSSHQDHVVSVELTKETQHTSSEHCSKGMQTDSSNSVSSSVSIQGF 600

Query: 601 EICDDAATPFSDITEPLFDHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRDKGNHGV-D 660
           EICDDAATPF+DI EP+FDHQ +EHDEIEE HPP CSP LQ E+SEH LL DKGNHGV D
Sbjct: 601 EICDDAATPFADIAEPVFDHQNIEHDEIEEIHPPVCSPSLQYEISEHNLLEDKGNHGVYD 660

Query: 661 AKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINMPSNNRPN 720
              T+ SFE SSDNVNLHNTEAG+KKESPS ELD PL SE RFIFKDDIPI+MP+N   N
Sbjct: 661 KSTTKCSFETSSDNVNLHNTEAGIKKESPSVELDHPLRSEGRFIFKDDIPISMPTNGS-N 720

Query: 721 MVTETNITSSFGDDKFTVRELLSSVAE-STALVASPSTSTHKCLLPDKVTTLHNPSAEKL 780
           MV+E    SS GDDKFTVRELLSSVAE +T LVASPS+STHKCLLPDKVTTLH  SAEKL
Sbjct: 721 MVSEQITISSHGDDKFTVRELLSSVAEPATPLVASPSSSTHKCLLPDKVTTLHTSSAEKL 780

Query: 781 ASSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840
           A SHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT
Sbjct: 781 AGSHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKDELELKNGTT 840

Query: 841 TTPLTLKSSGYSDAVCLKSNTSDHSSIKETSIGNPPSVPKTNSDSSEHTTKPITPVVDEE 900
           TTPLTLKSS  SDA+C K NTS+HS+IKE++  NPP +P TNSDS+EHT KP+TPVV+EE
Sbjct: 841 TTPLTLKSSSCSDALCSKPNTSEHSAIKESTGANPPMLPTTNSDSTEHT-KPVTPVVEEE 900

Query: 901 VPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960
           VPA  KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW
Sbjct: 901 VPA--KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIW 960

Query: 961 LAKSLKGMMIEDNSRSS 973
           LA+SLKGMMIEDN+RSS
Sbjct: 961 LARSLKGMMIEDNARSS 972

BLAST of MC02g0172 vs. ExPASy TrEMBL
Match: A0A5D3BAN0 (Serine/threonine-protein kinase Nek5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00280 PE=4 SV=1)

HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 855/1005 (85.07%), Postives = 911/1005 (90.65%), Query Frame = 0

Query: 1    MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
            MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA
Sbjct: 1    MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60

Query: 61   RVQHPYIVEFKEAWVEK-----------------------------GCYVCIVTGYCEGG 120
            RVQHPYIVEFKEAWVEK                             GCYVCIVTGYCEGG
Sbjct: 61   RVQHPYIVEFKEAWVEKVSIRFVNFLSLHGAKLSRKLLIVFDLQMQGCYVCIVTGYCEGG 120

Query: 121  DMAELMKKSNGMHFPEEKLCKWFTQLLLAVEYLHSNFVLHRDLKCSNIFLTKDQDVRLGD 180
            DMAELMKKSNGMHFPEEKLCKWFTQLLLAVEYLHSNFVLHRDLKCSNIFLTKD+DVRLGD
Sbjct: 121  DMAELMKKSNGMHFPEEKLCKWFTQLLLAVEYLHSNFVLHRDLKCSNIFLTKDKDVRLGD 180

Query: 181  FGLAKTLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAAHRPAFKAFD 240
            FGLAKTLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAAHRPAFKAFD
Sbjct: 181  FGLAKTLKADDLASSVVGTPNYMCPELLADIPYGFKSDIWSLGCCMYEMAAHRPAFKAFD 240

Query: 241  MAGLISKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPFIEQYRP 300
            MAGLISKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQP++EQYRP
Sbjct: 241  MAGLISKINRSSIGPLPSCYSPSLKTLIKGMLRKNPEHRPNASELLKHPYLQPYVEQYRP 300

Query: 301  SISPTVDFTPEKPRSARDSRRSMAESQNSNSSNSDKDSMVSSEKNTPATVSNCDDKVTES 360
            SISPTVDF+P+KPR  RDSRRSMAESQNSNSSNSD+DS+ SS+KNTPA  +NCDDKVT+S
Sbjct: 301  SISPTVDFSPDKPRLTRDSRRSMAESQNSNSSNSDRDSLFSSDKNTPAMTANCDDKVTDS 360

Query: 361  DMASVDDHDGSEPLLPCEE-QTHNICSLKVNDPMLTKPSQEDHQSNTESRRPKTIRNIMI 420
            DMASVDDH+GSEPLLPCEE QTHN+CSLKVNDPM +KPSQED QSN+ESRRPKTIRNIM+
Sbjct: 361  DMASVDDHEGSEPLLPCEEEQTHNVCSLKVNDPMHSKPSQED-QSNSESRRPKTIRNIML 420

Query: 421  ALKEGKVRENYSPIRGNRIKSGVASNQKAHAEVPPKVIKPPVAVPGSKSNADTPITVPAK 480
            ALKEGK RE+YSP+RGNR+KSGVASNQKA+AEVPPKVIKPP+AVPGSKS+ DTPITV AK
Sbjct: 421  ALKEGKARESYSPVRGNRVKSGVASNQKAYAEVPPKVIKPPMAVPGSKSSPDTPITVSAK 480

Query: 481  ANIESSRRIQVVATSKHQLPVVDPTPKTKPRHDSSPPLY-PVKQVEDGLSAKSRQRTPPN 540
            ANIESSRR QVV+T KHQLPVVDPTPK+KPRHDSSPPL  PVK VEDG+S+KSRQRTPP+
Sbjct: 481  ANIESSRRNQVVSTPKHQLPVVDPTPKSKPRHDSSPPLCRPVKPVEDGVSSKSRQRTPPS 540

Query: 541  LLRRPSFPARTRQVGL-DVPDAVSSMENIRQKETSEEGRTSNQVHSSHEDHVVSMELSKE 600
            LLRRPSFPARTRQVG  DVPDAVSS+E IRQKET  E RTS++VHSSH+DHVVS+EL+KE
Sbjct: 541  LLRRPSFPARTRQVGQQDVPDAVSSVERIRQKETLPEERTSHKVHSSHQDHVVSVELTKE 600

Query: 601  TQHNTSEHCSKGMQTDSSNSVSSSVSIQGFEICDDAATPFSDITEPLFDHQIVEHDEIEE 660
            TQH +SE CSKGMQTDSSNSVSSSVSIQGFEICDDAATPF+DI EP+FDHQ +EHDEIEE
Sbjct: 601  TQHISSEPCSKGMQTDSSNSVSSSVSIQGFEICDDAATPFADIAEPVFDHQSIEHDEIEE 660

Query: 661  THPPSCSPYLQSEMSEHKLLRDKGNHGVDAKPTERSFEISSDNVNLHNTEAGVKKESPSA 720
             HPP CSP LQ EMSEH LL DKGNHGV  KPT+ SFEISSD VNLH+TE G+KKESPS 
Sbjct: 661  IHPPVCSPSLQYEMSEHNLLEDKGNHGVYDKPTKCSFEISSDIVNLHDTEVGIKKESPSV 720

Query: 721  ELDLPLGSEERFIFKDDIPINMPSNNRPNMVTETNITSSFGDDKFTVRELLSSVAE-STA 780
            ELDLPL SE RF+FKDDIPINMP+N   NMV+E    SS GDDK+TVRELLSSVAE +T 
Sbjct: 721  ELDLPLRSEGRFVFKDDIPINMPTNGS-NMVSEKITISSHGDDKYTVRELLSSVAEPATP 780

Query: 781  LVASPSTSTHKCLLPDKVTTLHNPSAEKLASSHLPAAFDDVIHVIRHSSFRVGSEQPVTE 840
            LVASPS+STHKCLLPDKVTTLH  S EKLASSHLPAAFDDVIHVIRHSSFRVGSEQPVTE
Sbjct: 781  LVASPSSSTHKCLLPDKVTTLHTSSPEKLASSHLPAAFDDVIHVIRHSSFRVGSEQPVTE 840

Query: 841  NLEVGVQNVDVGKLVTVVKDELELKNGTTTTPLTLKSSGYSDAVCLKSNTSDHSSIKETS 900
            NLEVGVQNVDVGKLVTVVKDELELKNGTT TPLTLKS+  SDA+CLK NT +HS+IKE++
Sbjct: 841  NLEVGVQNVDVGKLVTVVKDELELKNGTTNTPLTLKSNSCSDALCLKPNTCEHSAIKEST 900

Query: 901  IGNPPSVPKTNSDSSEHTTKPITPVVDEEVPATAKETLDVKSFRQRAEALEGLLELSAEL 960
              NPP +P TNSDS+EHT KP+TPVV+EEVPA  KETLDVKSFRQRAEALEGLLELSAEL
Sbjct: 901  GANPPMLPTTNSDSTEHT-KPVTPVVEEEVPA--KETLDVKSFRQRAEALEGLLELSAEL 960

Query: 961  LQHNRLEELAVVLKPFGKDKVSPRETAIWLAKSLKGMMIEDNSRS 972
            LQHNRLEELAVVLKPFGKDKVSPRETAIWLAKSLKGMMIEDN+RS
Sbjct: 961  LQHNRLEELAVVLKPFGKDKVSPRETAIWLAKSLKGMMIEDNARS 1000

BLAST of MC02g0172 vs. TAIR 10
Match: AT3G44200.1 (NIMA (never in mitosis, gene A)-related 6 )

HSP 1 Score: 911.0 bits (2353), Expect = 8.2e-265
Identity = 556/1001 (55.54%), Postives = 670/1001 (66.93%), Query Frame = 0

Query: 1   MESRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIA 60
           MESRMDQYE+MEQIGRGAFGAAILV+HK+E+KKYVLKKIRLARQTERCRRSAHQEM+LIA
Sbjct: 1   MESRMDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIA 60

Query: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLA 120
           RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNG++FPEEKLCKWFTQLLLA
Sbjct: 61  RVQHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLA 120

Query: 121 VEYLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLA 180
           VEYLHSN+VLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDL SSVVGTPNYMCPELLA
Sbjct: 121 VEYLHSNYVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLA 180

Query: 181 DIPYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIK 240
           DIPYGFKSDIWSLGCC+YEMAA+RPAFKAFDMAGLISK+NRSSIGPLP CYSPSLK LIK
Sbjct: 181 DIPYGFKSDIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCYSPSLKALIK 240

Query: 241 GMLRKNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFTPEKPRSARDSRRSMAESQNS 300
           GMLRKNPE+RPNASE+LKHPYLQP++EQYRP++S     TPEKP ++R+ RRSMAESQNS
Sbjct: 241 GMLRKNPEYRPNASEILKHPYLQPYVEQYRPTLS-AASITPEKPLNSREGRRSMAESQNS 300

Query: 301 NSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEE--QTHNICSL 360
           NSS S+KD+   S+KN    V +  +KVTE+D   VDD D  + +    E     ++ + 
Sbjct: 301 NSS-SEKDNFYVSDKNIRYVVPSNGNKVTETDSGFVDDEDILDHVQQSAENGNLQSVSAT 360

Query: 361 KVNDPMLTKPSQEDHQSNT-ESRRPKTIRNIMIALKEGKVRENYSPIRGNRIKSGVASNQ 420
           K +   + KP   D + +  + R PKTIRNIM+ LKE K REN SP+R NR +      Q
Sbjct: 361 KPDGHGILKPVHSDQRPDVIQPRHPKTIRNIMMVLKEEKARENGSPMRSNRSRPSSVPTQ 420

Query: 421 KAHAEVPPKVIKPPVAVPGSKSNADTPITVPAKANIESSRRIQVVATSKHQLPVVDPTPK 480
           K + E P K+ K       SK+NA TPI  P       S R       KH +PV+D +PK
Sbjct: 421 KNNVETPSKIPKLGDIAHSSKTNASTPI--PPSKLASDSARTPGSFPPKHHMPVIDSSPK 480

Query: 481 TKPRHDSSPPLYPVK-QVEDGLSAKSRQRTPPNLLRRPSFPA-RTRQVGLDVPDAVSSME 540
            KPR+D   P    K + E+ +S K RQRTPP L RR S  A ++RQ+G D+        
Sbjct: 481 LKPRNDRISPSPAAKHEAEEAMSVKRRQRTPPTLPRRTSLIAHQSRQLGADI-------S 540

Query: 541 NIRQKETSE--EGRTSNQVHSSHEDHVVSMELSKETQ-HNTSEHCSKGMQTDSSNSVSSS 600
           N+  KET++      S    +SH+  V +  +S   +   TS   +KGMQ++SSNS+SSS
Sbjct: 541 NMAAKETAKLHPSVPSESETNSHQSRVHASPVSTTPEPKRTSVGSAKGMQSESSNSISSS 600

Query: 601 VSIQGFEICDDAATPFSDITEPLF--DHQIVEHDEIEETHPPSCSPYLQSEMSEHKLLRD 660
           +S+Q FE+CDDA+TP+ D+TE     DH+   H E   + P         ++S   ++R 
Sbjct: 601 LSMQAFELCDDASTPYIDMTEHTTPDDHRRSCHSEYSYSFP---------DISSEMMIR- 660

Query: 661 KGNHGVDAKPTERSFEISSDNVNLHNTEAGVKKESPSAELDLPLGSEERFIFKDDIPINM 720
           +  H    + TE    +S     + NT A  + E                + KDD P  +
Sbjct: 661 RDEHSTSMRLTEIPDSVS----GVQNTIAHHQPEREQGSCPT--------VLKDDSPATL 720

Query: 721 PSNNRPNMVTETNITSSFGDDKFTVRELLSSVAESTALVASPSTSTHKCLLPDKVTTL-- 780
            S   PN     +     GDDKFTV+E +SSV     L      S       D  T++  
Sbjct: 721 QS-YEPNTSQHQH-----GDDKFTVKEFVSSVPGPAPLPLHVEPSHQVNSHSDNKTSVMS 780

Query: 781 HNPSAEKLAS-SHLPAAFDDVIHVIRHSSFRVGSEQPVTENLEVGVQNVDVGKLVTVVKD 840
            N + EK  S SH     DDVIHVIRHSSFRVGS+QPV E++EVGVQNVD+GKL+ VV+D
Sbjct: 781 QNSALEKNNSHSHPHPVVDDVIHVIRHSSFRVGSDQPVMESVEVGVQNVDMGKLINVVRD 840

Query: 841 ELELKNGTTTTPLTLKSSGYS-------------DAVCLKSNTSDHSSIKETSIGNPPSV 900
           E+E++ G T +      S  S             D +   S T   +S  ++S    P+ 
Sbjct: 841 EMEVRKGATPSESPTTRSIISEPDSRTEPRPKEPDPITNYSETKSFNSCSDSS----PAE 900

Query: 901 PKTNSDSSEHTTKPITPVVDEEVPATAKETLDVKSFRQRAEALEGLLELSAELLQHNRLE 960
            +TNS   E  T P  PV         KETLD+KSFRQRAEALEGLLELSA+LL+ +RLE
Sbjct: 901 TRTNSFVPEEETTPTPPV---------KETLDIKSFRQRAEALEGLLELSADLLEQSRLE 949

Query: 961 ELAVVLKPFGKDKVSPRETAIWLAKSLKGMMIED--NSRSS 974
           ELA+VL+PFGK+KVSPRETAIWLAKSLKGMMIED  N+ SS
Sbjct: 961 ELAIVLQPFGKNKVSPRETAIWLAKSLKGMMIEDINNNNSS 949

BLAST of MC02g0172 vs. TAIR 10
Match: AT3G20860.1 (NIMA-related kinase 5 )

HSP 1 Score: 419.5 bits (1077), Expect = 7.5e-117
Identity = 211/331 (63.75%), Postives = 261/331 (78.85%), Query Frame = 0

Query: 3   SRMDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARV 62
           S+MD YE++EQIGRGAFG+A LV HKSE++KYV+KKIRLA+QTERC+ +A QEM+LI+++
Sbjct: 10  SKMDDYEVVEQIGRGAFGSAFLVIHKSERRKYVVKKIRLAKQTERCKLAAIQEMSLISKL 69

Query: 63  QHPYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLAVE 122
           + PYIVE+K++WVEK C VCIVT YCEGGDM +++KKS G+   EEKLC+W  QLLLA++
Sbjct: 70  KSPYIVEYKDSWVEKDC-VCIVTSYCEGGDMTQMIKKSRGVFASEEKLCRWMVQLLLAID 129

Query: 123 YLHSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADI 182
           YLH+N VLHRDLKCSNIFLTK+ +VRLGDFGLAK L  DDLASS+VGTPNYMCPELLADI
Sbjct: 130 YLHNNRVLHRDLKCSNIFLTKENEVRLGDFGLAKLLGKDDLASSMVGTPNYMCPELLADI 189

Query: 183 PYGFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGM 242
           PYG+KSDIWSLGCCM+E+AAH+PAFKA DMA LI+KINRSS+ PLP  YS SLK LIK M
Sbjct: 190 PYGYKSDIWSLGCCMFEVAAHQPAFKAPDMAALINKINRSSLSPLPVMYSSSLKRLIKSM 249

Query: 243 LRKNPEHRPNASELLKHPYLQPFIEQ-------YRPSISPTVDFT-------PEKPRSAR 302
           LRKNPEHRP A+ELL+HP+LQP++ Q       ++P +S +   T       P K +SA+
Sbjct: 250 LRKNPEHRPTAAELLRHPHLQPYLAQCQNLSPVFKPVVSKSEHNTNENRTGLPPKTKSAK 309

Query: 303 DSRRSMAESQNSNSSNSDKDSMVSSEKNTPA 320
              +   ES+ +   N D  S  S +K  PA
Sbjct: 310 TPIKHNQESEETEKKNKDTSSS-SKDKERPA 338

HSP 2 Score: 63.9 bits (154), Expect = 8.0e-10
Identity = 43/101 (42.57%), Postives = 58/101 (57.43%), Query Frame = 0

Query: 876 KTNSDSSEHTTKPITPVVDEEVPATAKETLDVKSFR-------QRAEALEGLLELSAELL 935
           K N D+S  +     P   +E+   +  TL ++ F+       +RAEALE LLEL A LL
Sbjct: 322 KKNKDTSSSSKDKERPAKSQEMSVISTLTL-LREFQKKSPKSEERAEALESLLELCAGLL 381

Query: 936 QHNRLEELAVVLKPFGKDKVSPRETAIWLAKSLKGMMIEDN 970
           +  + +EL  VLKPFG + VS RETAIWL KSL  +  + N
Sbjct: 382 RQEKFDELEGVLKPFGDETVSSRETAIWLTKSLMNVKRKQN 421

BLAST of MC02g0172 vs. TAIR 10
Match: AT3G04810.1 (NIMA-related kinase 2 )

HSP 1 Score: 416.8 bits (1070), Expect = 4.9e-116
Identity = 212/346 (61.27%), Postives = 266/346 (76.88%), Query Frame = 0

Query: 5   MDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+ YE++EQIG+G+FG+A+LV HK EKK YVLKKIRLARQT R RRSAHQEM LI+++ +
Sbjct: 1   MENYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIHN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCI+ GYC+GGDMAE +KK+NG+HF EEKLCKW  Q+LLA+EYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYL 120

Query: 125 HSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H+N +LHRD+KCSNIFLTKDQD+RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFLTKDQDIRLGDFGLAKVLTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCCMYEM A +PAFKAFDM GLI++INRS + PLP+ YS + + L+K MLR
Sbjct: 181 GSKSDIWSLGCCMYEMTAMKPAFKAFDMQGLINRINRSIVPPLPAQYSAAFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPFIEQYRPSIS-PTVDFTPEK-PRSARDSRRSMAESQNSNS 304
           KNPE RP+A+ELL+ P LQP+I++    ++ P  +  P + P S    R S  E +   +
Sbjct: 241 KNPELRPSAAELLRQPLLQPYIQKIHLKVNDPGSNVLPAQWPESESARRNSFPEQRRRPA 300

Query: 305 SNSD-----------KDSMVSSEKNTPATVSNCDDKVTESDMASVD 338
             S            +DS+ S +K  PA + N + +V  S  AS D
Sbjct: 301 GKSHSFGPSRFRGNLEDSVSSIKKTVPAYL-NRERQVDLSTDASGD 345

HSP 2 Score: 79.7 bits (195), Expect = 1.4e-14
Identity = 40/61 (65.57%), Postives = 48/61 (78.69%), Query Frame = 0

Query: 902 KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLAKSL 961
           ++  D  S++QRAEALEGLLE SA LLQ  R +EL V+L+PFG  KVSPRETAIWL+KS 
Sbjct: 537 RQRFDPSSYQQRAEALEGLLEFSARLLQDERYDELNVLLRPFGPGKVSPRETAIWLSKSF 596

Query: 962 K 963
           K
Sbjct: 597 K 597

BLAST of MC02g0172 vs. TAIR 10
Match: AT3G04810.2 (NIMA-related kinase 2 )

HSP 1 Score: 416.8 bits (1070), Expect = 4.9e-116
Identity = 212/346 (61.27%), Postives = 266/346 (76.88%), Query Frame = 0

Query: 5   MDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+ YE++EQIG+G+FG+A+LV HK EKK YVLKKIRLARQT R RRSAHQEM LI+++ +
Sbjct: 1   MENYEVLEQIGKGSFGSALLVRHKHEKKLYVLKKIRLARQTGRTRRSAHQEMELISKIHN 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEKGCYVCI+ GYC+GGDMAE +KK+NG+HF EEKLCKW  Q+LLA+EYL
Sbjct: 61  PFIVEYKDSWVEKGCYVCIIIGYCKGGDMAEAIKKTNGVHFTEEKLCKWLVQILLALEYL 120

Query: 125 HSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           H+N +LHRD+KCSNIFLTKDQD+RLGDFGLAK L +DDLASSVVGTP+YMCPELLADIPY
Sbjct: 121 HANHILHRDVKCSNIFLTKDQDIRLGDFGLAKVLTSDDLASSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCCMYEM A +PAFKAFDM GLI++INRS + PLP+ YS + + L+K MLR
Sbjct: 181 GSKSDIWSLGCCMYEMTAMKPAFKAFDMQGLINRINRSIVPPLPAQYSAAFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPFIEQYRPSIS-PTVDFTPEK-PRSARDSRRSMAESQNSNS 304
           KNPE RP+A+ELL+ P LQP+I++    ++ P  +  P + P S    R S  E +   +
Sbjct: 241 KNPELRPSAAELLRQPLLQPYIQKIHLKVNDPGSNVLPAQWPESESARRNSFPEQRRRPA 300

Query: 305 SNSD-----------KDSMVSSEKNTPATVSNCDDKVTESDMASVD 338
             S            +DS+ S +K  PA + N + +V  S  AS D
Sbjct: 301 GKSHSFGPSRFRGNLEDSVSSIKKTVPAYL-NRERQVDLSTDASGD 345

HSP 2 Score: 79.7 bits (195), Expect = 1.4e-14
Identity = 40/61 (65.57%), Postives = 48/61 (78.69%), Query Frame = 0

Query: 902 KETLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRETAIWLAKSL 961
           ++  D  S++QRAEALEGLLE SA LLQ  R +EL V+L+PFG  KVSPRETAIWL+KS 
Sbjct: 537 RQRFDPSSYQQRAEALEGLLEFSARLLQDERYDELNVLLRPFGPGKVSPRETAIWLSKSF 596

Query: 962 K 963
           K
Sbjct: 597 K 597

BLAST of MC02g0172 vs. TAIR 10
Match: AT1G54510.1 (NIMA-related serine/threonine kinase 1 )

HSP 1 Score: 413.3 bits (1061), Expect = 5.4e-115
Identity = 215/390 (55.13%), Postives = 283/390 (72.56%), Query Frame = 0

Query: 5   MDQYEIMEQIGRGAFGAAILVNHKSEKKKYVLKKIRLARQTERCRRSAHQEMALIARVQH 64
           M+QYE +EQIG+G+FG+A+LV HK EKKKYVLKKIRLARQT+R RRSAHQEM LI++++H
Sbjct: 1   MEQYEFLEQIGKGSFGSALLVRHKHEKKKYVLKKIRLARQTQRTRRSAHQEMELISKMRH 60

Query: 65  PYIVEFKEAWVEKGCYVCIVTGYCEGGDMAELMKKSNGMHFPEEKLCKWFTQLLLAVEYL 124
           P+IVE+K++WVEK CYVCIV GYCEGGDMA+ +KKSNG+HF EEKLCKW  QLL+ +EYL
Sbjct: 61  PFIVEYKDSWVEKACYVCIVIGYCEGGDMAQAIKKSNGVHFQEEKLCKWLVQLLMGLEYL 120

Query: 125 HSNFVLHRDLKCSNIFLTKDQDVRLGDFGLAKTLKADDLASSVVGTPNYMCPELLADIPY 184
           HSN +LHRD+KCSNIFLTK+QD+RLGDFGLAK L +DDL SSVVGTP+YMCPELLADIPY
Sbjct: 121 HSNHILHRDVKCSNIFLTKEQDIRLGDFGLAKILTSDDLTSSVVGTPSYMCPELLADIPY 180

Query: 185 GFKSDIWSLGCCMYEMAAHRPAFKAFDMAGLISKINRSSIGPLPSCYSPSLKTLIKGMLR 244
           G KSDIWSLGCC+YEMA  +PAFKAFDM  LI+KIN++ + PLP+ YS   + L+K MLR
Sbjct: 181 GSKSDIWSLGCCIYEMAYLKPAFKAFDMQALINKINKTIVSPLPAKYSGPFRGLVKSMLR 240

Query: 245 KNPEHRPNASELLKHPYLQPFIEQYRPSISPTVDFT--PEKPRSARDSRR---------- 304
           KNPE RP+AS+LL+HP+LQP++   +  ++     T  PE P S R  ++          
Sbjct: 241 KNPEVRPSASDLLRHPHLQPYVLDVKLRLNNLRRKTLPPELPSSKRIMKKAHFSEPAVTC 300

Query: 305 -SMAESQNSNSSNSDKDSMVSSEKNTPATVSNCDDKVTESDMASVDDHDGSEPLLPCEEQ 364
            +  E Q+ +  N D+     +E++T +++     ++++  + S      S+  L C++ 
Sbjct: 301 PAFGERQHRSLWN-DRALNPEAEEDTASSIKCISRRISDLSIES-----SSKGTLICKQV 360

Query: 365 THNICSLKVNDPMLTKPSQEDHQSNTESRR 382
           + + C  KV+   L K S    +     RR
Sbjct: 361 SSSAC--KVSKYPLAKSSVTSRRIMETGRR 382

HSP 2 Score: 82.0 bits (201), Expect = 2.8e-15
Identity = 57/129 (44.19%), Postives = 76/129 (58.91%), Query Frame = 0

Query: 838 TPLTLKSSGYSDAVCLKSNTSDHSSIK--ETSIGNPPSVPKTNSDSSEHTTKPITPVVDE 897
           TP+  K+S  S +V  +S T D  +++   T  G   ++    SD S  + +  T     
Sbjct: 461 TPMQRKTSSKSSSVSDRSITKDKCTVQTHTTWQGIQLNMVDNISDGSSSSDQNATAGASS 520

Query: 898 EVPATAKE--TLDVKSFRQRAEALEGLLELSAELLQHNRLEELAVVLKPFGKDKVSPRET 957
              +++      D  S+RQRA+ALEGLLE SA LLQ  R +EL V+LKPFG  KVSPRET
Sbjct: 521 HTTSSSSRRCRFDPSSYRQRADALEGLLEFSARLLQEGRYDELNVLLKPFGPGKVSPRET 580

Query: 958 AIWLAKSLK 963
           AIW+AKSLK
Sbjct: 581 AIWIAKSLK 589