MC02g0157 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g0157 vs. ExPASy Swiss-Prot
Match: Q93WX6 (Cysteine desulfurase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NFS2 PE=1 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 9.8e-200
Identity = 356/474 (75.11%), Postives = 400/474 (84.39%), Query Frame = 0

Query: 2   MEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHFA 61
           ME V +KLPSF    P+  S    S  R        R SS  SV ++A+ ++ T S    
Sbjct: 1   MEGVAMKLPSF----PNAISIGHRSFSR-------VRCSSSLSVCSAAAASSATISTDSE 60

Query: 62  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHY 121
           S+SLGH  R DF ILHQEVNGSKLVYLD+AATSQKP AVL ALQNYYE YNSNVHRGIHY
Sbjct: 61  SVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHY 120

Query: 122 LSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTV 181
           LSAKATDE+ELARKKVA F+NA+++REI+FTRNATEAINLVAYSWGL+NLK  DE+ILTV
Sbjct: 121 LSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTV 180

Query: 182 AEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASV 241
           AEHHS IVPWQ+V+++TGAVLKFV+L+E +VP +  L+E+ + KTKLV  HHVSNVLAS 
Sbjct: 181 AEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASS 240

Query: 242 LPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKS 301
           LPIE+IV WAH  GAKVLVDACQSVPHMVVDVQ L+ADFLVASSHKMCGPTGIGFLYGKS
Sbjct: 241 LPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGKS 300

Query: 302 DLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQK 361
           DLL +MPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAI LGAAVDYLSGIGM K
Sbjct: 301 DLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMPK 360

Query: 362 IHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHG 421
           IH YE E+ +YLYE L ++P++RIYGP P+ +V R ALCSFNVE +HPTD+ATFLDQQHG
Sbjct: 361 IHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTDLATFLDQQHG 420

Query: 422 VAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 476
           VAIRSGHHCAQPLHR LGV++SARASLYFYNTK+DVD FI AL DTVSFFNSFK
Sbjct: 421 VAIRSGHHCAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTVSFFNSFK 463

BLAST of MC02g0157 vs. ExPASy Swiss-Prot
Match: Q55793 (Probable cysteine desulfurase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=csd PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.8e-148
Identity = 260/409 (63.57%), Postives = 309/409 (75.55%), Query Frame = 0

Query: 64  SLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLS 123
           SL  T R DFPIL+QE+NG  LVYLDNAATSQKP AVL+ L +YYE  N+NVHRG H LS
Sbjct: 8   SLAATVRQDFPILNQEINGHPLVYLDNAATSQKPRAVLEKLMHYYENDNANVHRGAHQLS 67

Query: 124 AKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAE 183
            +ATD YE  R KVA F+NA   REI++TRNATEAINLVAYSWG+ NLK+ DEII TV E
Sbjct: 68  VRATDAYEAVRNKVAKFINARSPREIVYTRNATEAINLVAYSWGMNNLKAGDEIITTVME 127

Query: 184 HHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLP 243
           HHS +VPWQ+VA +TGAVLKFV LDE +   L   K + + KTKLV   H+SN L  V P
Sbjct: 128 HHSNLVPWQMVAAKTGAVLKFVQLDEQESFDLEHFKTLLSEKTKLVTVVHISNTLGCVNP 187

Query: 244 IEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDL 303
            E+I   AH+ GAKVLVDACQS PH  +DVQ +D D+LVAS HKMC PTGIGFLYGK ++
Sbjct: 188 AEEIAQLAHQAGAKVLVDACQSAPHYPLDVQLIDCDWLVASGHKMCAPTGIGFLYGKEEI 247

Query: 304 LSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIH 363
           L AMPPF GGGEMI++VF DH T  E P +FEAGTPAI EAI LGAAVDYL+ +GM+ IH
Sbjct: 248 LEAMPPFFGGGEMIAEVFFDHFTTGELPHKFEAGTPAIAEAIALGAAVDYLTDLGMENIH 307

Query: 364 SYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVA 423
           +YE EL  YL++ L  +P +R+YGP P  +  RAAL SFNV  +H +D+AT +DQ  G+A
Sbjct: 308 NYEVELTHYLWQGLGQIPQLRLYGPNP-KHGDRAALASFNVAGLHASDVATMVDQD-GIA 367

Query: 424 IRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 473
           IRSGHHC QPLHR    S SARASLYFYNTKE++D F+Q+L  T+ FF+
Sbjct: 368 IRSGHHCTQPLHRLFDASGSARASLYFYNTKEEIDLFLQSLQATIRFFS 414

BLAST of MC02g0157 vs. ExPASy Swiss-Prot
Match: O32164 (Cysteine desulfurase SufS OS=Bacillus subtilis (strain 168) OX=224308 GN=sufS PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 3.2e-118
Identity = 211/404 (52.23%), Postives = 277/404 (68.56%), Query Frame = 0

Query: 70  RPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKATDE 129
           R  FPILHQ+VNG  LVYLD+AATSQKP AV++ L  YY  YNSNVHRG+H L  +ATD 
Sbjct: 7   REQFPILHQQVNGHDLVYLDSAATSQKPRAVIETLDKYYNQYNSNVHRGVHTLGTRATDG 66

Query: 130 YELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIV 189
           YE AR+KV  F+NA    EIIFT+  T ++N+VA S+  ANLK  DE+++T  EHH+ I+
Sbjct: 67  YEGAREKVRKFINAKSMAEIIFTKGTTTSLNMVALSYARANLKPGDEVVITYMEHHANII 126

Query: 190 PWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIEDIVG 249
           PWQ   + TGA LK++ L E     L D++E  T+ TK+V   HVSNVL +V PI+++  
Sbjct: 127 PWQQAVKATGATLKYIPLQEDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNPIKEMAK 186

Query: 250 WAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSAMPP 309
            AH  GA ++VD  QS PHM +DVQ LD DF   SSHKMCGPTG+G LYGK  LL  M P
Sbjct: 187 IAHDNGAVIVVDGAQSTPHMKIDVQDLDCDFFALSSHKMCGPTGVGVLYGKKALLENMEP 246

Query: 310 FLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYEEEL 369
              GGEMI  V L  ST+ E P +FEAGTP I  AIGLGAA+D+L  IG+ +I  +E +L
Sbjct: 247 AEFGGEMIDFVGLYESTWKELPWKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKL 306

Query: 370 ARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRSGHH 429
           A Y  E  R +  + +YGP      +RA L +FN++++HP D+AT LD + G+A+R+GHH
Sbjct: 307 AAYALERFRQLDGVTVYGPE-----ERAGLVTFNLDDVHPHDVATVLDAE-GIAVRAGHH 366

Query: 430 CAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNS 474
           CAQPL + L V+++ARAS Y YNT+E++D  ++AL  T  +F +
Sbjct: 367 CAQPLMKWLDVTATARASFYLYNTEEEIDKLVEALQKTKEYFTN 404

BLAST of MC02g0157 vs. ExPASy Swiss-Prot
Match: Q9K7A0 (Probable cysteine desulfurase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=csd PE=3 SV=2)

HSP 1 Score: 422.2 bits (1084), Expect = 8.0e-117
Identity = 215/405 (53.09%), Postives = 276/405 (68.15%), Query Frame = 0

Query: 67  HTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKA 126
           H  +  FP+L QEVNGS LVYLD+AATSQKPIAV++AL +YY  YNSNVHRG+H L   A
Sbjct: 4   HEIKKLFPVLDQEVNGSPLVYLDSAATSQKPIAVIEALDDYYRRYNSNVHRGVHTLGTLA 63

Query: 127 TDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHS 186
           TD YE AR+K+  F++A+   EIIFTR  T AINLVA S+G ANL   DEI++T  EHHS
Sbjct: 64  TDGYEGAREKIRRFIHASSTEEIIFTRGTTTAINLVAASYGRANLGEGDEIVITPMEHHS 123

Query: 187 AIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIED 246
            I+PWQ VA+ TGA L ++ L +    K+ D+++  + KTK+V   HVSNVL ++ P+++
Sbjct: 124 NIIPWQQVAKATGATLTYLPLQKDGTIKIEDVEKTISEKTKIVAIMHVSNVLGTINPVKE 183

Query: 247 IVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSA 306
           I   AHR GA +LVD  QS PHM +DVQ L  DF   S HKM GPTGIG LYGK   L  
Sbjct: 184 IAEIAHRHGAIMLVDGAQSAPHMKIDVQELGCDFFAFSGHKMAGPTGIGVLYGKKAHLEK 243

Query: 307 MPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYE 366
           M P   GGEMI  V L  ST+ E P +FE GTP I  AIGLGAA+D+L  IG+ +I  +E
Sbjct: 244 MEPVEFGGEMIDFVGLYDSTWKELPWKFEGGTPIIAGAIGLGAAIDFLEDIGLDEIEKHE 303

Query: 367 EELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRS 426
            ELA+Y  + L  +  + +YGP      +RA L +FN+E++HP D+AT LD   G+A+R+
Sbjct: 304 HELAQYALDRLSELEGMTVYGPQ-----KRAGLVTFNIEDVHPHDVATVLDAD-GIAVRA 363

Query: 427 GHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFF 472
           GHHCAQPL + L V+++ARAS Y YNTKED+D   + L  T  +F
Sbjct: 364 GHHCAQPLMKWLDVTATARASFYLYNTKEDIDALAKGLEKTKEYF 402

BLAST of MC02g0157 vs. ExPASy Swiss-Prot
Match: Q8CTA4 (Probable cysteine desulfurase OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=csd PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.1e-113
Identity = 207/401 (51.62%), Postives = 270/401 (67.33%), Query Frame = 0

Query: 72  DFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKATDEYE 131
           DFPIL Q+VN  +L YLD+ ATSQ P+ VL  L +YY+ YNSNVHRG+H L + ATD YE
Sbjct: 14  DFPILEQKVNNKRLAYLDSTATSQTPVQVLNVLDDYYKRYNSNVHRGVHTLGSLATDGYE 73

Query: 132 LARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIVPW 191
            AR+ V  F+NA    EIIFTR  T +IN+VA+S+G AN+   DEI++T  EHH+ IVPW
Sbjct: 74  NARETVRRFINAKYFEEIIFTRGTTASINIVAHSYGDANISEGDEIVVTEMEHHANIVPW 133

Query: 192 QLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIEDIVGWA 251
           Q +A+R  A LKF+ + +    +L D+K     KTK+V   HVSNVL ++  ++ I   A
Sbjct: 134 QQLAKRKNATLKFIPMTKDGELQLDDIKATINDKTKIVAIAHVSNVLGTINDVKTIAKIA 193

Query: 252 HRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSAMPPFL 311
           H  GA + VD  QS PHM +D+Q +DADF   S HKM GPTGIG LYGK +LL  M P  
Sbjct: 194 HEHGAVISVDGAQSAPHMALDMQDIDADFYSFSGHKMLGPTGIGVLYGKRELLQNMEPVE 253

Query: 312 GGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYEEELAR 371
            GG+MI  V    ST+A+ P++FEAGTP I +AIGL  A+ Y+  +G   IH +E+EL  
Sbjct: 254 FGGDMIDFVSKYDSTWADLPTKFEAGTPLIAQAIGLAEAIHYIENLGFNAIHQHEKELTE 313

Query: 372 YLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRSGHHCA 431
           Y YE +  +  + IYGP      +RA + +FN+ +IHP D+AT +D + GVA+R+GHHCA
Sbjct: 314 YAYEQMLTIDGLEIYGPPKD---RRAGVITFNLADIHPHDVATAVDTE-GVAVRAGHHCA 373

Query: 432 QPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 473
           QPL + LGVSS+ARAS Y YNTKEDVD  +QAL  T  FF+
Sbjct: 374 QPLMKWLGVSSTARASFYVYNTKEDVDQLVQALKQTKEFFS 410

BLAST of MC02g0157 vs. NCBI nr
Match: XP_022153071.1 (cysteine desulfurase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF 60
           MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF
Sbjct: 1   MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF 60

Query: 61  ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH 120
           ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH
Sbjct: 61  ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH 120

Query: 121 YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT 180
           YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT
Sbjct: 121 YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT 180

Query: 181 VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS 240
           VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS
Sbjct: 181 VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS 240

Query: 241 VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK 300
           VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK
Sbjct: 241 VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK 300

Query: 301 SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ 360
           SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ
Sbjct: 301 SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ 360

Query: 361 KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH 420
           KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH
Sbjct: 361 KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH 420

Query: 421 GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 475
           GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK
Sbjct: 421 GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 475

BLAST of MC02g0157 vs. NCBI nr
Match: XP_023513282.1 (cysteine desulfurase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 860 bits (2222), Expect = 3.00e-313
Identity = 441/483 (91.30%), Postives = 457/483 (94.62%), Query Frame = 0

Query: 1   MMEVVVLKLPSFQF----INPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASASTA 60
           MME VVLKLPSFQF    INPSRCSSCFSSTLR S S +LR    RFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQLLFINPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 61  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120
            ETD+LH  SLSLGH+TRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHSTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 121 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 180
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 240
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEMFTTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMFTTKTKLVVTH 240

Query: 241 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300
           HVSNVLASVLPIEDIV WAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 301 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 360
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 361 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 420
           YLSGIGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N +RAALCSFN+EN+HPTDI
Sbjct: 361 YLSGIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAERAALCSFNIENMHPTDI 420

Query: 421 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 475
           ATFLDQQHG+AIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFD 480

BLAST of MC02g0157 vs. NCBI nr
Match: XP_022943969.1 (cysteine desulfurase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 857 bits (2214), Expect = 4.96e-312
Identity = 440/483 (91.10%), Postives = 456/483 (94.41%), Query Frame = 0

Query: 1   MMEVVVLKLPSFQF----INPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASASTA 60
           MME VVLKLPSFQF    INPSRCSSCFSSTLR S S +LR    RFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQLHFINPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 61  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120
            ETD+LH  SLSLGH+TRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHSTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 121 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 180
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 240
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEMFTTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMFTTKTKLVVTH 240

Query: 241 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300
           HVSNVLASVLPIEDIV WAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 301 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 360
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 361 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 420
           YLS IGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALCSFN+EN+HPTDI
Sbjct: 361 YLSEIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALCSFNIENMHPTDI 420

Query: 421 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 475
           ATFLDQQHG+AIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTV+FF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVNFFD 480

BLAST of MC02g0157 vs. NCBI nr
Match: XP_022986219.1 (cysteine desulfurase 1, chloroplastic-like, partial [Cucurbita maxima])

HSP 1 Score: 849 bits (2193), Expect = 7.01e-309
Identity = 435/480 (90.62%), Postives = 451/480 (93.96%), Query Frame = 0

Query: 1   MMEVVVLKLPSFQF----INPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASASTA 60
           MME VVLKLPSFQF    +NPSRCSSCFSSTLR S S +LR    RFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQFHFMNPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 61  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120
            ETD+LH  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 121 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 180
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 240
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEM TTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMLTTKTKLVVTH 240

Query: 241 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300
           HVSNVLASVLPIEDIV WAH FGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHHFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 301 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 360
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 361 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 420
           YLSGIGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALCSFN++N+HPTDI
Sbjct: 361 YLSGIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALCSFNIDNMHPTDI 420

Query: 421 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 472
           ATFLDQQHG+AIRSGHHCAQPLHR LGVSSSARASLYFYNTKEDVDYFIQALNDTVSFF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRVLGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFD 478

BLAST of MC02g0157 vs. NCBI nr
Match: XP_008449615.1 (PREDICTED: cysteine desulfurase 1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 832 bits (2150), Expect = 3.24e-302
Identity = 430/485 (88.66%), Postives = 450/485 (92.78%), Query Frame = 0

Query: 1   MMEVVVLKLPSF------QFINPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASAS 60
           MME +VLKLPSF      Q +NP+RC SCFSSTLR S S  LR    RFSSP SVSASAS
Sbjct: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60

Query: 61  TATETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEA 120
           TA ETDSLH  SLSLGHTTRPDFPILHQE+NGSKLVYLDNAATSQKPI+VL ALQNYYEA
Sbjct: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120

Query: 121 YNSNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYE ARKKVASF+NA +A+EIIFTRNATEAINLVAYSWGLAN
Sbjct: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVV 240
           LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSL EHDVP L D KEMF+TKTKLVV
Sbjct: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240

Query: 241 THHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCG 300
           THHVSNVLASVLPIE+IVG AHRFGAKVLVDACQSVPHMVV+VQALDADFLVASSHKMCG
Sbjct: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 300

Query: 301 PTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAA 360
           PTGIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAIGLGAA
Sbjct: 301 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 360

Query: 361 VDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPT 420
           +DYLSGIGM KIHSYEEELA YLYE+LRAVPNIRIYGPAPTA+VQRAALCSFN+ +IHPT
Sbjct: 361 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 420

Query: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSF 475
           DIATFLDQQHGVAIRSGHHCAQPLHRALGV+SSAR SLYFYNTKEDVDYFIQALNDTVSF
Sbjct: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 480

BLAST of MC02g0157 vs. ExPASy TrEMBL
Match: A0A6J1DJL0 (Cysteine desulfurase OS=Momordica charantia OX=3673 GN=LOC111020663 PE=4 SV=1)

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF 60
           MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF
Sbjct: 1   MMEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHF 60

Query: 61  ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH 120
           ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH
Sbjct: 61  ASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIH 120

Query: 121 YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT 180
           YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT
Sbjct: 121 YLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILT 180

Query: 181 VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS 240
           VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS
Sbjct: 181 VAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLAS 240

Query: 241 VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK 300
           VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK
Sbjct: 241 VLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGK 300

Query: 301 SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ 360
           SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ
Sbjct: 301 SDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQ 360

Query: 361 KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH 420
           KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH
Sbjct: 361 KIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQH 420

Query: 421 GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 475
           GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK
Sbjct: 421 GVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 475

BLAST of MC02g0157 vs. ExPASy TrEMBL
Match: A0A6J1FVQ2 (Cysteine desulfurase OS=Cucurbita moschata OX=3662 GN=LOC111448537 PE=4 SV=1)

HSP 1 Score: 857 bits (2214), Expect = 2.40e-312
Identity = 440/483 (91.10%), Postives = 456/483 (94.41%), Query Frame = 0

Query: 1   MMEVVVLKLPSFQF----INPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASASTA 60
           MME VVLKLPSFQF    INPSRCSSCFSSTLR S S +LR    RFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQLHFINPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 61  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120
            ETD+LH  SLSLGH+TRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHSTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 121 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 180
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 240
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEMFTTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMFTTKTKLVVTH 240

Query: 241 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300
           HVSNVLASVLPIEDIV WAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 301 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 360
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 361 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 420
           YLS IGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALCSFN+EN+HPTDI
Sbjct: 361 YLSEIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALCSFNIENMHPTDI 420

Query: 421 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 475
           ATFLDQQHG+AIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTV+FF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVNFFD 480

BLAST of MC02g0157 vs. ExPASy TrEMBL
Match: A0A6J1JFG5 (Cysteine desulfurase OS=Cucurbita maxima OX=3661 GN=LOC111484037 PE=4 SV=1)

HSP 1 Score: 849 bits (2193), Expect = 3.39e-309
Identity = 435/480 (90.62%), Postives = 451/480 (93.96%), Query Frame = 0

Query: 1   MMEVVVLKLPSFQF----INPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASASTA 60
           MME VVLKLPSFQF    +NPSRCSSCFSSTLR S S +LR    RFSSPFSVSASASTA
Sbjct: 1   MMEGVVLKLPSFQFQFHFMNPSRCSSCFSSTLRTSSSPSLRFRFRRFSSPFSVSASASTA 60

Query: 61  TETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120
            ETD+LH  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN
Sbjct: 61  RETDNLHLGSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYN 120

Query: 121 SNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLK 180
           SNVHRGIHYLSAKATDEYELARKKVASF+NA +AREI+FTRNATEAINLVAYSWG+ANLK
Sbjct: 121 SNVHRGIHYLSAKATDEYELARKKVASFINAGDAREIVFTRNATEAINLVAYSWGIANLK 180

Query: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTH 240
           SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVP L D KEM TTKTKLVVTH
Sbjct: 181 SEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPNLKDFKEMLTTKTKLVVTH 240

Query: 241 HVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300
           HVSNVLASVLPIEDIV WAH FGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT
Sbjct: 241 HVSNVLASVLPIEDIVSWAHHFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPT 300

Query: 301 GIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVD 360
           GIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+AEPPSRFEAGTPAIGEAIGLGAA+D
Sbjct: 301 GIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAID 360

Query: 361 YLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDI 420
           YLSGIGMQKIH+YEEELA YLYE+LRAVPNIRIYGP P  N QRAALCSFN++N+HPTDI
Sbjct: 361 YLSGIGMQKIHTYEEELANYLYENLRAVPNIRIYGPVP--NAQRAALCSFNIDNMHPTDI 420

Query: 421 ATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFN 472
           ATFLDQQHG+AIRSGHHCAQPLHR LGVSSSARASLYFYNTKEDVDYFIQALNDTVSFF+
Sbjct: 421 ATFLDQQHGIAIRSGHHCAQPLHRVLGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFD 478

BLAST of MC02g0157 vs. ExPASy TrEMBL
Match: A0A1S3BLT6 (Cysteine desulfurase OS=Cucumis melo OX=3656 GN=LOC103491447 PE=4 SV=1)

HSP 1 Score: 832 bits (2150), Expect = 1.57e-302
Identity = 430/485 (88.66%), Postives = 450/485 (92.78%), Query Frame = 0

Query: 1   MMEVVVLKLPSF------QFINPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASAS 60
           MME +VLKLPSF      Q +NP+RC SCFSSTLR S S  LR    RFSSP SVSASAS
Sbjct: 1   MMEGMVLKLPSFHSQFQFQLMNPNRCISCFSSTLRASSSPLLRFGNPRFSSPLSVSASAS 60

Query: 61  TATETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEA 120
           TA ETDSLH  SLSLGHTTRPDFPILHQE+NGSKLVYLDNAATSQKPI+VL ALQNYYEA
Sbjct: 61  TAKETDSLHLNSLSLGHTTRPDFPILHQEINGSKLVYLDNAATSQKPISVLNALQNYYEA 120

Query: 121 YNSNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH LSAKATDEYE ARKKVASF+NA +A+EIIFTRNATEAINLVAYSWGLAN
Sbjct: 121 YNSNVHRGIHSLSAKATDEYERARKKVASFINAGDAKEIIFTRNATEAINLVAYSWGLAN 180

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVV 240
           LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSL EHDVP L D KEMF+TKTKLVV
Sbjct: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLGEHDVPNLKDFKEMFSTKTKLVV 240

Query: 241 THHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCG 300
           THHVSNVLASVLPIE+IVG AHRFGAKVLVDACQSVPHMVV+VQALDADFLVASSHKMCG
Sbjct: 241 THHVSNVLASVLPIEEIVGLAHRFGAKVLVDACQSVPHMVVNVQALDADFLVASSHKMCG 300

Query: 301 PTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAA 360
           PTGIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAIGLGAA
Sbjct: 301 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 360

Query: 361 VDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPT 420
           +DYLSGIGM KIHSYEEELA YLYE+LRAVPNIRIYGPAPTA+VQRAALCSFN+ +IHPT
Sbjct: 361 IDYLSGIGMTKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 420

Query: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSF 475
           DIATFLDQQHGVAIRSGHHCAQPLHRALGV+SSAR SLYFYNTKEDVDYFIQALNDTVSF
Sbjct: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVNSSARVSLYFYNTKEDVDYFIQALNDTVSF 480

BLAST of MC02g0157 vs. ExPASy TrEMBL
Match: A0A0A0KEG9 (Cysteine desulfurase OS=Cucumis sativus OX=3659 GN=Csa_6G427980 PE=4 SV=1)

HSP 1 Score: 828 bits (2139), Expect = 2.20e-300
Identity = 428/485 (88.25%), Postives = 449/485 (92.58%), Query Frame = 0

Query: 1   MMEVVVLKLPSF------QFINPSRCSSCFSSTLRNSPSSALR----RFSSPFSVSASAS 60
           MME +VLK PSF      QF+NP+RC SCFSSTLR S S  LR    R  S FSVSASAS
Sbjct: 30  MMEGMVLKSPSFHSQFHFQFMNPNRCISCFSSTLRASSSPLLRFRNPRLPSHFSVSASAS 89

Query: 61  TATETDSLHFASLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEA 120
           TA ETDSLH  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPI+VL ALQNYY+A
Sbjct: 90  TAKETDSLHLNSLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPISVLNALQNYYQA 149

Query: 121 YNSNVHRGIHYLSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLAN 180
           YNSNVHRGIH+LSAKATDEYE ARKKVASF+NA +A+EI+FTRNATEAINLVAYSWGLAN
Sbjct: 150 YNSNVHRGIHFLSAKATDEYERARKKVASFINAGDAKEIVFTRNATEAINLVAYSWGLAN 209

Query: 181 LKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVV 240
           LKS DEIILTVAEHHSAIVPWQLVAERTGAVLKFV+L EHDVP L DLKEMF+TKTKLVV
Sbjct: 210 LKSGDEIILTVAEHHSAIVPWQLVAERTGAVLKFVNLGEHDVPNLKDLKEMFSTKTKLVV 269

Query: 241 THHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCG 300
           THHVSNVLASVLPI +IVG AHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCG
Sbjct: 270 THHVSNVLASVLPIGEIVGLAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCG 329

Query: 301 PTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAA 360
           PTGIGFLYGK DLLSAMPPFLGGGEMISDVFLDHST+A+PPSRFEAGTPAIGEAIGLGAA
Sbjct: 330 PTGIGFLYGKIDLLSAMPPFLGGGEMISDVFLDHSTFAQPPSRFEAGTPAIGEAIGLGAA 389

Query: 361 VDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPT 420
           +DYLSGIGMQKIHSYEEELA YLYE+LRAVPNIRIYGPAPTA+VQRAALCSFN+ +IHPT
Sbjct: 390 IDYLSGIGMQKIHSYEEELANYLYENLRAVPNIRIYGPAPTAHVQRAALCSFNIGDIHPT 449

Query: 421 DIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSF 475
           DIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSAR SLYFYNTKEDVDYFIQALNDTVSF
Sbjct: 450 DIATFLDQQHGVAIRSGHHCAQPLHRALGVSSSARVSLYFYNTKEDVDYFIQALNDTVSF 509

BLAST of MC02g0157 vs. TAIR 10
Match: AT1G08490.1 (chloroplastic NIFS-like cysteine desulfurase )

HSP 1 Score: 697.6 bits (1799), Expect = 7.0e-201
Identity = 356/474 (75.11%), Postives = 400/474 (84.39%), Query Frame = 0

Query: 2   MEVVVLKLPSFQFINPSRCSSCFSSTLRNSPSSALRRFSSPFSVSASASTATETDSLHFA 61
           ME V +KLPSF    P+  S    S  R        R SS  SV ++A+ ++ T S    
Sbjct: 1   MEGVAMKLPSF----PNAISIGHRSFSR-------VRCSSSLSVCSAAAASSATISTDSE 60

Query: 62  SLSLGHTTRPDFPILHQEVNGSKLVYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHY 121
           S+SLGH  R DF ILHQEVNGSKLVYLD+AATSQKP AVL ALQNYYE YNSNVHRGIHY
Sbjct: 61  SVSLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHY 120

Query: 122 LSAKATDEYELARKKVASFVNAAEAREIIFTRNATEAINLVAYSWGLANLKSEDEIILTV 181
           LSAKATDE+ELARKKVA F+NA+++REI+FTRNATEAINLVAYSWGL+NLK  DE+ILTV
Sbjct: 121 LSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTV 180

Query: 182 AEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASV 241
           AEHHS IVPWQ+V+++TGAVLKFV+L+E +VP +  L+E+ + KTKLV  HHVSNVLAS 
Sbjct: 181 AEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASS 240

Query: 242 LPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKS 301
           LPIE+IV WAH  GAKVLVDACQSVPHMVVDVQ L+ADFLVASSHKMCGPTGIGFLYGKS
Sbjct: 241 LPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGKS 300

Query: 302 DLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQK 361
           DLL +MPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAI LGAAVDYLSGIGM K
Sbjct: 301 DLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMPK 360

Query: 362 IHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHG 421
           IH YE E+ +YLYE L ++P++RIYGP P+ +V R ALCSFNVE +HPTD+ATFLDQQHG
Sbjct: 361 IHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTDLATFLDQQHG 420

Query: 422 VAIRSGHHCAQPLHRALGVSSSARASLYFYNTKEDVDYFIQALNDTVSFFNSFK 476
           VAIRSGHHCAQPLHR LGV++SARASLYFYNTK+DVD FI AL DTVSFFNSFK
Sbjct: 421 VAIRSGHHCAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTVSFFNSFK 463

BLAST of MC02g0157 vs. TAIR 10
Match: AT5G65720.1 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 89.0 bits (219), Expect = 1.1e-17
Identity = 92/388 (23.71%), Postives = 165/388 (42.53%), Query Frame = 0

Query: 86  VYLDNAATSQKPIAVLKALQNYYEAYNSNVHRGIHYLSAKATDEYELARKKVASFVNAAE 145
           +YLD  AT+     V  A+         N H   H    +A +  E AR +VA  + A+ 
Sbjct: 55  LYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEAS- 114

Query: 146 AREIIFTRNATEAINLVAYSWGLANLKSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFV 205
            +EI+F   ATEA N+ A    +   K   + ++T    H  ++      ++ G  + ++
Sbjct: 115 PKEIVFVSGATEANNM-AVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYL 174

Query: 206 SLDEHDVPKLADLKEMFTTKTKLVVTHHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQS 265
            +    +  L  L+E     T LV    V+N +  V P+E+I             DA Q+
Sbjct: 175 PVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQA 234

Query: 266 VPHMVVDVQALDADFLVASSHKMCGPTGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHS 325
           +  + VDV+  +   +  S+HK+ GP G+G LY +      + P + GG           
Sbjct: 235 IGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQ--------- 294

Query: 326 TYAEPPSRFEAGTPAIGEAIGLGAAVDYLSGIGMQKIHSYEEELARYLYESLRAVPNIRI 385
                     +GT A  + +G GAA +    + M+++  Y+E+  + L E L      ++
Sbjct: 295 -----ERGLRSGTGATQQIVGFGAACE----LAMKEM-EYDEKWIKGLQERLLNGVREKL 354

Query: 386 YGPAPTANVQRAALCSFNVENIHPTDIATFLDQQHGVAIRSGHHCA----QPLH--RALG 445
            G     ++    + + N+   +    +  +  +  VA+ SG  C     +P +  RALG
Sbjct: 355 DGVVVNGSMDSRYVGNLNLSFAYVEGESLLMGLKE-VAVSSGSACTSASLEPSYVLRALG 414

Query: 446 VS-----SSARASLYFYNTKEDVDYFIQ 463
           V      +S R  +  + TKE++D  ++
Sbjct: 415 VDEDMAHTSIRFGIGRFTTKEEIDKAVE 420

BLAST of MC02g0157 vs. TAIR 10
Match: AT5G65720.2 (nitrogen fixation S (NIFS)-like 1 )

HSP 1 Score: 66.6 bits (161), Expect = 6.0e-11
Identity = 66/302 (21.85%), Postives = 127/302 (42.05%), Query Frame = 0

Query: 172 KSEDEIILTVAEHHSAIVPWQLVAERTGAVLKFVSLDEHDVPKLADLKEMFTTKTKLVVT 231
           K   + ++T    H  ++      ++ G  + ++ +    +  L  L+E     T LV  
Sbjct: 11  KDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTDGLVDLEMLREAIRPDTGLVSI 70

Query: 232 HHVSNVLASVLPIEDIVGWAHRFGAKVLVDACQSVPHMVVDVQALDADFLVASSHKMCGP 291
             V+N +  V P+E+I             DA Q++  + VDV+  +   +  S+HK+ GP
Sbjct: 71  MAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQAIGKIPVDVKKWNVALMSMSAHKIYGP 130

Query: 292 TGIGFLYGKSDLLSAMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIGLGAAV 351
            G+G LY +      + P + GG                     +GT A  + +G GAA 
Sbjct: 131 KGVGALYVRRRPRIRLEPLMNGGGQ--------------ERGLRSGTGATQQIVGFGAAC 190

Query: 352 DYLSGIGMQKIHSYEEELARYLYESLRAVPNIRIYGPAPTANVQRAALCSFNVENIHPTD 411
           +    + M+++  Y+E+  + L E L      ++ G     ++    + + N+   +   
Sbjct: 191 E----LAMKEM-EYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNLSFAYVEG 250

Query: 412 IATFLDQQHGVAIRSGHHCA----QPLH--RALGVS-----SSARASLYFYNTKEDVDYF 463
            +  +  +  VA+ SG  C     +P +  RALGV      +S R  +  + TKE++D  
Sbjct: 251 ESLLMGLKE-VAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTKEEIDKA 292