MC02g0093 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g0093 vs. ExPASy Swiss-Prot
Match: Q6H7M3 (Copper-transporting ATPase HMA4 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA4 PE=1 SV=1)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 559/881 (63.45%), Postives = 715/881 (81.16%), Query Frame = 0

Query: 21  SRTLR-IIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAESIENALMMVDGVKRAVVGIT 80
           +RT++  IE  NF+V+   EQ+IA CRL+IKGM CTSC+ES+E AL MV GVK+A VG+ 
Sbjct: 88  ARTIKEAIEGLNFEVDELQEQEIAVCRLQIKGMACTSCSESVERALQMVPGVKKAAVGLA 147

Query: 81  IGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKVHVKLDEVNF-EDMITIRTS 140
           + EAKV FD ++ + D ++EA++DAGF  +LI+S D+ NKVH+KL+ V+  ED+  I++ 
Sbjct: 148 LEEAKVHFDPNITSRDLIIEAIEDAGFGADLISSGDDVNKVHLKLEGVSSPEDIKLIQSR 207

Query: 141 LENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDI 200
           LE+  GVN VE D + Q + ++Y+PD TGPR LIQCI++AAQ  + +NASLY PP++R+ 
Sbjct: 208 LESVEGVNNVECDTAGQTIIVAYDPDVTGPRLLIQCIQDAAQPPKYFNASLYSPPKQREA 267

Query: 201 EQLEEIRTHRNLFLLSCLFSVPVVLL-MVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCT 260
           E+  EIR +RN FL SCLFSVPV +  MVLPM+ P+G+WL Y V N +T+GMLL+W+LC+
Sbjct: 268 ERHHEIRNYRNQFLWSCLFSVPVFMFSMVLPMISPFGDWLFYKVCNNMTIGMLLRWLLCS 327

Query: 261 PVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFE 320
           PVQFI+GWRFYVG+Y+AL++  +NMDVLVALGTNAAYFYSVYIV+KA  S+ F+G+DFFE
Sbjct: 328 PVQFIIGWRFYVGAYHALKRGYSNMDVLVALGTNAAYFYSVYIVLKALTSESFEGQDFFE 387

Query: 321 TSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQML 380
           TSAMLISFILLGKYLEV+AKGKTSDAL+KL  LAP+T+CLLTLD + N ++E EI TQ+L
Sbjct: 388 TSAMLISFILLGKYLEVVAKGKTSDALSKLTELAPETACLLTLDKDGNAISETEISTQLL 447

Query: 381 QRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFV 440
           QRNDVIKIVPG KVPVDG+VI GQSHVNES ITGE+RP  KKPGDKV+ GTVN+NGC+ V
Sbjct: 448 QRNDVIKIVPGEKVPVDGVVIKGQSHVNESMITGEARPIAKKPGDKVIGGTVNDNGCIIV 507

Query: 441 KATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEA 500
           K THVG++T LSQIVQLVE+AQL+RA  QKLAD+IS+FFVP VVV AFLTWLGW + G+ 
Sbjct: 508 KVTHVGSETALSQIVQLVEAAQLARAPVQKLADRISRFFVPTVVVAAFLTWLGWFVAGQF 567

Query: 501 GIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGAS 560
            IYP+ WIPK MD FELALQFGISVLV+ACPCALGLATPTAVMVA+GKGAS GVLIKG +
Sbjct: 568 DIYPREWIPKAMDSFELALQFGISVLVVACPCALGLATPTAVMVATGKGASQGVLIKGGN 627

Query: 561 ALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVV 620
           ALE A+KVK ++FDKTGTLT+G+P VV   +FS   + ELC +A   E+NS+HPL+ A+V
Sbjct: 628 ALEKAHKVKAIIFDKTGTLTVGKPSVVQTKVFSKIPLLELCDLAAGAEANSEHPLSKAIV 687

Query: 621 QHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEV 680
           ++ KK++++YG  ++     ++F V PG GV   V+ K L LVGN+RLM+   + + +EV
Sbjct: 688 EYTKKLREQYGSHSDHIMESKDFEVHPGAGVSANVEGK-LVLVGNKRLMQEFEVPISSEV 747

Query: 681 DRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRF 740
           + ++++ E  A+TC+LVAI+  + G   V+DP KP A   ISYL S GISSIMVTGDN  
Sbjct: 748 EGHMSETEELARTCVLVAIDRTICGALSVSDPLKPEAGRAISYLSSMGISSIMVTGDNWA 807

Query: 741 TAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIG 800
           TA ++A+++GI  V +E+DP+GKA KIK L+M G  VAMVGDG+NDSPALAA+D+G+AIG
Sbjct: 808 TAKSIAKEVGIGTVFAEIDPVGKAEKIKDLQMKGLTVAMVGDGINDSPALAAADVGLAIG 867

Query: 801 AGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYP 860
           AGTD+AIEAADIVLMRS++EDV+TAIDLSR+T  RI  NY+WALGYN++ +P+AAG+L+P
Sbjct: 868 AGTDVAIEAADIVLMRSSLEDVITAIDLSRKTLSRIRLNYVWALGYNVLGMPVAAGVLFP 927

Query: 861 FTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQSI 899
           FTG RLPPWLAGACMAASSVSVVCSSLLL+ +K+PL+ + +
Sbjct: 928 FTGIRLPPWLAGACMAASSVSVVCSSLLLQLYKKPLHVEEV 967

BLAST of MC02g0093 vs. ExPASy Swiss-Prot
Match: Q9SH30 (Probable copper-transporting ATPase HMA5 OS=Arabidopsis thaliana OX=3702 GN=HMA5 PE=1 SV=2)

HSP 1 Score: 884.8 bits (2285), Expect = 8.2e-256
Identity = 480/860 (55.81%), Postives = 619/860 (71.98%), Query Frame = 0

Query: 39  EQDIATCRLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVL 98
           E+    CR+RI GMTCTSC+ +IE  L  V+GV+RA V + I EA++ +D  L + DR+L
Sbjct: 125 ERSRQVCRIRINGMTCTSCSSTIERVLQSVNGVQRAHVALAIEEAEIHYDPRLSSYDRLL 184

Query: 99  EAVDDAGFDVELINSADEGNKVHVKLD-EVNFEDMITIRTSLENAVGVNLVEMDFSQQKV 158
           E +++AGF+  LI++ ++ +K+ +K+D E+  E M  I  SLE   GV  VE+     K+
Sbjct: 185 EEIENAGFEAVLISTGEDVSKIDLKIDGELTDESMKVIERSLEALPGVQSVEISHGTDKI 244

Query: 159 TISYEPDQTGPRFLIQCIEEAAQGLRTY-NASLYLPPR-RRDIEQLEEIRTHRNLFLLSC 218
           ++ Y+PD TGPR  IQ IE    G   +  A+++      R+ ++  EI+ +   FL S 
Sbjct: 245 SVLYKPDVTGPRNFIQVIESTVFGHSGHIKATIFSEGGVGRESQKQGEIKQYYKSFLWSL 304

Query: 219 LFSVPVVL-LMVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYA 278
           +F+VPV L  MV   +P   + L + V NMLTVG +++ +L TPVQF++GWRFY GSY A
Sbjct: 305 VFTVPVFLTAMVFMYIPGIKDLLMFKVINMLTVGEIIRCVLATPVQFVIGWRFYTGSYKA 364

Query: 279 LRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLEV 338
           LR+ SANMDVL+ALGTNAAYFYS+Y V++A  S  F+G DFFETSAMLISFI+LGKYLEV
Sbjct: 365 LRRGSANMDVLIALGTNAAYFYSLYTVLRAATSPDFKGVDFFETSAMLISFIILGKYLEV 424

Query: 339 IAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVD 398
           +AKGKTS A+AKL  LAPDT+ LL+LD   N+  E EID +++Q+NDVIKIVPGAKV  D
Sbjct: 425 MAKGKTSQAIAKLMNLAPDTAILLSLDKEGNVTGEEEIDGRLIQKNDVIKIVPGAKVASD 484

Query: 399 GIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQL 458
           G VI GQSHVNES ITGE+RP  K+ GD V+ GT+NENG L VK T VG+++ L+QIV+L
Sbjct: 485 GYVIWGQSHVNESMITGEARPVAKRKGDTVIGGTLNENGVLHVKVTRVGSESALAQIVRL 544

Query: 459 VESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFEL 518
           VESAQL++A  QKLAD+ISKFFVP V+ ++F TWL W + G+   YP+ WIP  MD FEL
Sbjct: 545 VESAQLAKAPVQKLADRISKFFVPLVIFLSFSTWLAWFLAGKLHWYPESWIPSSMDSFEL 604

Query: 519 ALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASALENAYKVKTVVFDKTG 578
           ALQFGISV+VIACPCALGLATPTAVMV +G GAS GVLIKG  ALE A+KV  +VFDKTG
Sbjct: 605 ALQFGISVMVIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALERAHKVNCIVFDKTG 664

Query: 579 TLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHAKKVQQKYGFSAERP 638
           TLT+G+P VV   L  N  + E   +  A E NS+HPLA A+V++AKK +       E P
Sbjct: 665 TLTMGKPVVVKTKLLKNMVLREFYELVAATEVNSEHPLAKAIVEYAKKFRD----DEENP 724

Query: 639 GHVE--NFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDRYIADNEHKAQTCI 698
              E  +F    G GV   V  + + +VGN+ LM  + + +  + +  +AD+E  AQT I
Sbjct: 725 AWPEACDFVSITGKGVKATVKGREI-MVGNKNLMNDHKVIIPDDAEELLADSEDMAQTGI 784

Query: 699 LVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTAAALARDLGIDDVL 758
           LV+IN  + G   V+DP KP A+  IS L+S  I SIMVTGDN  TA ++AR++GID V+
Sbjct: 785 LVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSIMVTGDNWGTANSIAREVGIDSVI 844

Query: 759 SEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAGTDIAIEAADIVLM 818
           +E  P  KA K+K L+  G +VAMVGDG+NDSPAL A+D+G+AIGAGTDIAIEAADIVLM
Sbjct: 845 AEAKPEQKAEKVKELQAAGHVVAMVGDGINDSPALVAADVGMAIGAGTDIAIEAADIVLM 904

Query: 819 RSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFTGTRLPPWLAGACM 878
           +SN+EDV+TAIDLSR+T  RI  NY+WALGYN++ +PIAAG+L+P T  RLPPW+AGA M
Sbjct: 905 KSNLEDVITAIDLSRKTFSRIRLNYVWALGYNLMGIPIAAGVLFPGTRFRLPPWIAGAAM 964

Query: 879 AASSVSVVCSSLLLKCFKRP 893
           AASSVSVVC SLLLK +KRP
Sbjct: 965 AASSVSVVCCSLLLKNYKRP 979

BLAST of MC02g0093 vs. ExPASy Swiss-Prot
Match: A3AWA4 (Copper-transporting ATPase HMA5 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA5 PE=2 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 8.0e-251
Identity = 463/860 (53.84%), Postives = 616/860 (71.63%), Query Frame = 0

Query: 39  EQDIATCRLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVL 98
           E++I  CRL IKGMTCTSCA ++E+ L +V GV+RA V +   EA++ +D  ++   ++ 
Sbjct: 149 EKNILVCRLHIKGMTCTSCASTVESILQVVPGVQRASVALATEEAEIRYDRRIVTASQLT 208

Query: 99  EAVDDAGFDVELINSADEGNKVHVKLD-EVNFEDMITIRTSLENAVGVNLVEMDFSQQKV 158
            AV++ GF+  LI + D+ +++ +K+D  +N   ++ +++S++   GV  +++D    K+
Sbjct: 209 HAVEETGFEAILITTGDDQSRIDLKVDGTLNERSIMIVKSSVQALPGVEDIKVDPELHKI 268

Query: 159 TISYEPDQTGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLF 218
           TISY+PDQTGPR LI+ IE AA G  T   S+Y     R   +  EI+ +R  FL S +F
Sbjct: 269 TISYKPDQTGPRDLIEVIESAASGDLT--VSIYPEADGRQQHRHGEIKRYRQSFLWSLVF 328

Query: 219 SVPVVLL-MVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALR 278
           ++PV L  MV   +P   + L+  V NM+++G LL+WIL TPVQF++G RFY G+Y AL 
Sbjct: 329 TIPVFLTSMVFMYIPGLKDGLEKKVINMMSIGELLRWILSTPVQFVIGRRFYTGAYKALS 388

Query: 279 QKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIA 338
             S+NMDVL+ALGTN AYFYSVY +++A +S  +   DFFETS+MLISFILLGKYLE++A
Sbjct: 389 HGSSNMDVLIALGTNTAYFYSVYSILRAASSHNYMATDFFETSSMLISFILLGKYLEILA 448

Query: 339 KGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGI 398
           KGKTS+A+AKL  LAP+T+ +L  D   N++ E EID++++Q+NDVIK+VPG KV  DG 
Sbjct: 449 KGKTSEAIAKLMDLAPETATMLIYDHEGNVVGEKEIDSRLIQKNDVIKVVPGGKVASDGF 508

Query: 399 VIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVE 458
           VI GQSHVNES ITGESRP  K+ GD V+ GTVNENG L V+AT VG+++ L+QIV+LVE
Sbjct: 509 VIWGQSHVNESMITGESRPVAKRKGDTVIGGTVNENGVLHVRATFVGSESALAQIVRLVE 568

Query: 459 SAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELAL 518
           SAQ+++A  QK ADQIS+ FVP V++++ LTWL W + G    YP  WIP  MD F+LAL
Sbjct: 569 SAQMAKAPVQKFADQISRVFVPLVIILSLLTWLAWFLAGRLHGYPNSWIPSSMDSFQLAL 628

Query: 519 QFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASALENAYKVKTVVFDKTGTL 578
           QFGISV+VIACPCALGLATPTAVMVA+G GAS GVLIKG  ALE+A KV  +VFDKTGTL
Sbjct: 629 QFGISVMVIACPCALGLATPTAVMVATGVGASQGVLIKGGQALESAQKVDCIVFDKTGTL 688

Query: 579 TLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGH 638
           T+G+P VV+  L  N  + E      A E NS+HPL  AVV+HAKK      F +E   H
Sbjct: 689 TIGKPVVVNTRLLKNMVLREFYAYVAAAEVNSEHPLGKAVVEHAKK------FHSEE-SH 748

Query: 639 V----ENFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDRYIADNEHKAQTCI 698
           V     +F    G GV  K+  +   +VGN+  M  + I +  E    + + E KAQT I
Sbjct: 749 VWTEARDFISVTGHGVKAKISGR-AVMVGNKSFMLTSGIDIPVEALEILTEEEEKAQTAI 808

Query: 699 LVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTAAALARDLGIDDVL 758
           +VA++  V G   V+DP KP A+ VISYL+S  + SIMVTGDN  TA A+++++GI++ +
Sbjct: 809 IVAMDQEVVGIISVSDPIKPNAREVISYLKSMKVESIMVTGDNWGTANAISKEVGIENTV 868

Query: 759 SEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAGTDIAIEAADIVLM 818
           +E  P  KA K+K L+  G  VAMVGDG+NDSPAL ++D+G+AIGAGTD+AIEAADIVLM
Sbjct: 869 AEAKPEQKAEKVKELQSAGRTVAMVGDGINDSPALVSADVGLAIGAGTDVAIEAADIVLM 928

Query: 819 RSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFTGTRLPPWLAGACM 878
           +SN+EDV+TAIDLSR+T +RI  NY+WALGYNII +PIAAG+L+P T  RLPPW+AGA M
Sbjct: 929 KSNLEDVITAIDLSRKTFFRIRMNYVWALGYNIIGIPIAAGVLFPSTRFRLPPWVAGAAM 988

Query: 879 AASSVSVVCSSLLLKCFKRP 893
           AASSVSVVC SLLL+ +K P
Sbjct: 989 AASSVSVVCWSLLLRYYKSP 998

BLAST of MC02g0093 vs. ExPASy Swiss-Prot
Match: A0A0P0X004 (Cation-transporting ATPase HMA5 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA9 PE=2 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 4.9e-200
Identity = 392/869 (45.11%), Postives = 558/869 (64.21%), Query Frame = 0

Query: 40  QDIATCRLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLE 99
           Q   + + RI GMTC +C  S+E  L  + GVK AVV +     +V +D S+IN+D ++E
Sbjct: 130 QKTLSAQFRIGGMTCANCVNSVEGILKRLSGVKGAVVALATSLGEVEYDPSVINKDEIVE 189

Query: 100 AVDDAGFDVELINSADEGNKVHVKLDEVNFE-DMITIRTSLENAVGVNLVEMDFSQQKVT 159
           A++DAGF+   + S+++ +K+ + L  ++ E D+  +   L+  +G+   +++ +  +V 
Sbjct: 190 AIEDAGFEAAFLQSSEQ-DKILLGLTGLHTERDVNVLHDILKKMIGLRQFDVNATVSEVE 249

Query: 160 ISYEPDQTGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFS 219
           I ++P+  G R ++  IE  + G     A +  P  R       E     +L   S   S
Sbjct: 250 IIFDPEAVGLRSIVDAIETGSNG--RLKAHVQNPYARGASNDAHEAAKMLHLLRSSLFLS 309

Query: 220 VPVVLL-MVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQ 279
           +PV  + MV P + P+   +  M      +G LLKWIL + VQF+VG RFY+ +Y ALR 
Sbjct: 310 IPVFFIRMVCPHI-PFIRSILMMHCGPFHMGDLLKWILVSIVQFVVGKRFYIAAYRALRH 369

Query: 280 KSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAK 339
            S NMDVLV LGT A+Y YSV  ++   A   F    +FETSAM+I+F+L GKYLEV+AK
Sbjct: 370 GSTNMDVLVVLGTTASYVYSVCALLYG-AFTGFHPPIYFETSAMIITFVLFGKYLEVLAK 429

Query: 340 GKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIV 399
           GKTSDA+ KL  L P T+ LL  D       E EID  ++Q  D++K++PG+KVP DG+V
Sbjct: 430 GKTSDAIKKLVELVPATALLLLKDKEGKYTEEREIDALLVQPGDILKVLPGSKVPADGVV 489

Query: 400 IHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVES 459
           + G SHVNES ITGES P  K+    V+ GT+N +G L ++A  VG++T LSQI+ LVE+
Sbjct: 490 VWGTSHVNESMITGESAPIPKEVSSAVIGGTMNLHGVLHIQANKVGSETVLSQIISLVET 549

Query: 460 AQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQ 519
           AQ+S+A  QK AD ++  FVP V+ ++ +T+L W +CG  G YP  WI    + F  +L 
Sbjct: 550 AQMSKAPIQKFADYVASIFVPIVITLSMITFLVWFLCGWVGAYPNSWISGTSNCFVFSLM 609

Query: 520 FGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLT 579
           F I+V+VIACPCALGLATPTAVMVA+G GA+ GVL+KG  ALE A  V  V+FDKTGTLT
Sbjct: 610 FAIAVVVIACPCALGLATPTAVMVATGVGANHGVLVKGGDALERAQNVNYVIFDKTGTLT 669

Query: 580 LGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHA-------KKVQQKYGFS 639
            G+  V +  +FS   + +   +  + E++S+HPLA A+V++A       K    K G  
Sbjct: 670 QGKAVVTTAKVFSGMDLGDFLTLVASAEASSEHPLAKAIVEYAFHFHFFGKLPTSKDGIE 729

Query: 640 AERPG-------HVENFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDRYIAD 699
             +          VE+F+  PG GV   ++ K   LVGNR L+  N + +  E + ++ D
Sbjct: 730 QRKEDRLSQLLLQVEDFSALPGKGVQCLINGK-RVLVGNRTLVTENGVNVPPEAENFLVD 789

Query: 700 NEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTAAALA 759
            E  A+T ILV+ +    G  G+ DP K  A  V+  L+  G+  +M+TGDN  TA A+A
Sbjct: 790 LELNAKTGILVSYDDDFVGLMGITDPLKREAAVVVEGLKKMGVHPVMLTGDNWRTAKAVA 849

Query: 760 RDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAGTDIA 819
           +++GI+DV +EV P GKA+ +++L+  G IVAMVGDG+NDSPALAA+D+G+AIG GTDIA
Sbjct: 850 KEVGIEDVRAEVMPAGKADVVRSLQKDGSIVAMVGDGINDSPALAAADVGMAIGGGTDIA 909

Query: 820 IEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFTGTRL 879
           IEAAD VL+R+N+EDV+TAIDLSR+T  RI +NY +A+ YN++ +P+AAG L+PFT  ++
Sbjct: 910 IEAADYVLVRNNLEDVITAIDLSRKTFSRIRWNYFFAMAYNVVAIPVAAGALFPFTRLQM 969

Query: 880 PPWLAGACMAASSVSVVCSSLLLKCFKRP 893
           PPWLAGACMA SSVSVVCSSLLL+ +++P
Sbjct: 970 PPWLAGACMAFSSVSVVCSSLLLRRYRKP 992

BLAST of MC02g0093 vs. ExPASy Swiss-Prot
Match: Q9S7J8 (Copper-transporting ATPase RAN1 OS=Arabidopsis thaliana OX=3702 GN=RAN1 PE=1 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 1.4e-199
Identity = 396/874 (45.31%), Postives = 558/874 (63.84%), Query Frame = 0

Query: 39  EQDIATC--RLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDR 98
           EQ  AT   +  I GMTC +C  S+E  L  + GVKRAVV ++    +V +D ++IN+D 
Sbjct: 127 EQTQATLVGQFTIGGMTCAACVNSVEGILRDLPGVKRAVVALSTSLGEVEYDPNVINKDD 186

Query: 99  VLEAVDDAGFDVELINSADEGNKVHVKLDEV-NFEDMITIRTSLENAVGVNLVEMDFSQQ 158
           ++ A++DAGF+  L+ S ++ +K+ +++D + N  D   +   L    GV    +D    
Sbjct: 187 IVNAIEDAGFEGSLVQS-NQQDKLVLRVDGILNELDAQVLEGILTRLNGVRQFRLDRISG 246

Query: 159 KVTISYEPDQTGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSC 218
           ++ + ++P+    R L+  IEE   G   +   +  P  R   +   E       F+ S 
Sbjct: 247 ELEVVFDPEVVSSRSLVDGIEE--DGFGKFKLRVMSPYERLSSKDTGEASNMFRRFISSL 306

Query: 219 LFSVPVVLLMVL-PMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYA 278
           + S+P+  + V+ P +  +   L +       +G  LKW L + +QF++G RFYV ++ A
Sbjct: 307 VLSIPLFFIQVICPHIALFDALLVWRC-GPFMMGDWLKWALVSVIQFVIGKRFYVAAWRA 366

Query: 279 LRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLEV 338
           LR  S NMDVLVALGT+A+YFYSV  ++   A   F    +F+ SAMLI+F+LLGKYLE 
Sbjct: 367 LRNGSTNMDVLVALGTSASYFYSVGALLYG-AVTGFWSPTYFDASAMLITFVLLGKYLES 426

Query: 339 IAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVD 398
           +AKGKTSDA+ KL +L P T+ LLT      ++ E EID  ++Q  D +K+ PGAK+P D
Sbjct: 427 LAKGKTSDAMKKLVQLTPATAILLTEGKGGKLVGEREIDALLIQPGDTLKVHPGAKIPAD 486

Query: 399 GIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQL 458
           G+V+ G S+VNES +TGES P  K+    V+ GT+N +G L +KAT VG+D  LSQI+ L
Sbjct: 487 GVVVWGSSYVNESMVTGESVPVSKEVDSPVIGGTINMHGALHMKATKVGSDAVLSQIISL 546

Query: 459 VESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFEL 518
           VE+AQ+S+A  QK AD ++  FVP V+ +A  T +GW I G  G YP  W+P+    F  
Sbjct: 547 VETAQMSKAPIQKFADYVASIFVPVVITLALFTLVGWSIGGAVGAYPDEWLPENGTHFVF 606

Query: 519 ALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASALENAYKVKTVVFDKTG 578
           +L F ISV+VIACPCALGLATPTAVMVA+G GA+ GVLIKG  ALE A+KVK V+FDKTG
Sbjct: 607 SLMFSISVVVIACPCALGLATPTAVMVATGVGATNGVLIKGGDALEKAHKVKYVIFDKTG 666

Query: 579 TLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHAK------------- 638
           TLT G+  V +  +FS     E   +  + E++S+HPLA A+V +A+             
Sbjct: 667 TLTQGKATVTTTKVFSEMDRGEFLTLVASAEASSEHPLAKAIVAYARHFHFFDESTEDGE 726

Query: 639 ---KVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVD 698
              K  Q  G+  +      +F+  PG G+   V+ K + LVGNR+LM  N I +   V+
Sbjct: 727 TNNKDLQNSGWLLD----TSDFSALPGKGIQCLVNEKMI-LVGNRKLMSENAINIPDHVE 786

Query: 699 RYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFT 758
           +++ D E   +T ++VA NG++ G  G+ DP K  A  V+  L   G+  IMVTGDN  T
Sbjct: 787 KFVEDLEESGKTGVIVAYNGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMVTGDNWRT 846

Query: 759 AAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGA 818
           A A+A+++GI+DV +EV P GKA+ I++L+  G  VAMVGDG+NDSPALAA+D+G+AIGA
Sbjct: 847 ARAVAKEVGIEDVRAEVMPAGKADVIRSLQKDGSTVAMVGDGINDSPALAAADVGMAIGA 906

Query: 819 GTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPF 878
           GTD+AIEAAD VLMR+N+EDV+TAIDLSR+T  RI  NY++A+ YN++ +PIAAG+ +P 
Sbjct: 907 GTDVAIEAADYVLMRNNLEDVITAIDLSRKTLTRIRLNYVFAMAYNVVSIPIAAGVFFPV 966

Query: 879 TGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRP 893
              +LPPW AGACMA SSVSVVCSSLLL+ +K+P
Sbjct: 967 LRVQLPPWAAGACMALSSVSVVCSSLLLRRYKKP 990

BLAST of MC02g0093 vs. NCBI nr
Match: XP_022148856.1 (copper-transporting ATPase HMA4-like [Momordica charantia] >XP_022148857.1 copper-transporting ATPase HMA4-like [Momordica charantia])

HSP 1 Score: 1745 bits (4520), Expect = 0.0
Identity = 899/899 (100.00%), Postives = 899/899 (100.00%), Query Frame = 0

Query: 1   MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES 60
           MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES
Sbjct: 1   MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES 60

Query: 61  IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV 120
           IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV
Sbjct: 61  IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV 120

Query: 121 HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ 180
           HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ
Sbjct: 121 HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ 180

Query: 181 GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLLMVLPMLPPYGEWLDYM 240
           GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLLMVLPMLPPYGEWLDYM
Sbjct: 181 GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLLMVLPMLPPYGEWLDYM 240

Query: 241 VYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYI 300
           VYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYI
Sbjct: 241 VYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYI 300

Query: 301 VIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTL 360
           VIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTL
Sbjct: 301 VIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTL 360

Query: 361 DDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKP 420
           DDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKP
Sbjct: 361 DDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKP 420

Query: 421 GDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAV 480
           GDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAV
Sbjct: 421 GDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAV 480

Query: 481 VVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVM 540
           VVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVM
Sbjct: 481 VVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVM 540

Query: 541 VASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHV 600
           VASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHV
Sbjct: 541 VASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHV 600

Query: 601 AMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALV 660
           AMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALV
Sbjct: 601 AMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALV 660

Query: 661 GNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISY 720
           GNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISY
Sbjct: 661 GNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISY 720

Query: 721 LRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDG 780
           LRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDG
Sbjct: 721 LRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDG 780

Query: 781 VNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWA 840
           VNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWA
Sbjct: 781 VNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWA 840

Query: 841 LGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQSII 899
           LGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQSII
Sbjct: 841 LGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQSII 899

BLAST of MC02g0093 vs. NCBI nr
Match: XP_022149488.1 (copper-transporting ATPase HMA4-like [Momordica charantia])

HSP 1 Score: 1298 bits (3359), Expect = 0.0
Identity = 664/898 (73.94%), Postives = 772/898 (85.97%), Query Frame = 0

Query: 1   MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES 60
           M++ LD+L+ PLNP   ++ +R + II+AG F  + +L QD+  CRL+I+ MT  + A+S
Sbjct: 1   METKLDDLKIPLNPTTLNNENRAVEIIDAGGFPADEFL-QDVEICRLKIRSMTFRANAQS 60

Query: 61  IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV 120
           ++NAL  V GVKR V+   I EA V FD +  NE++++EA+  +GF+VEL+  AD   KV
Sbjct: 61  LQNALTTVHGVKRTVIAPEIEEATVHFDPNSTNEEQIIEAISGSGFEVELLTFADNPYKV 120

Query: 121 HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ 180
           H+KL+ V+FEDM  IR+SLE A GVN VEMD   QKV I Y+PDQTGPR L+QC      
Sbjct: 121 HIKLERVSFEDMAAIRSSLEQACGVNFVEMDVVGQKVAIGYDPDQTGPRSLLQC------ 180

Query: 181 GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLL-MVLPMLPPYGEWLDY 240
            L++Y ASLY+PPRRRD++QL+E+ T+RNLFLLSCLFSVPVV   MVLPMLPPYG+WL+Y
Sbjct: 181 -LKSYRASLYVPPRRRDVQQLQEVCTYRNLFLLSCLFSVPVVAFAMVLPMLPPYGDWLNY 240

Query: 241 MVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVY 300
             YNMLTVGM+L+WI CTPVQFI G RFYVGSY+ALRQKSANMDVLVALGTNAAYFYSVY
Sbjct: 241 RAYNMLTVGMVLRWIFCTPVQFIAGRRFYVGSYHALRQKSANMDVLVALGTNAAYFYSVY 300

Query: 301 IVIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLT 360
           I+ KAF S  F+G+DFFETS+MLISFILLGKYLEV+AKGKTSDALAKLA LAPDT+CL+T
Sbjct: 301 ILFKAFTSTSFRGQDFFETSSMLISFILLGKYLEVMAKGKTSDALAKLAHLAPDTACLMT 360

Query: 361 LDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKK 420
           L+D+ N+L+E+EIDTQ++QRND++KIVPGAKVPVDGIVI GQSHVNESTITGE++  +K 
Sbjct: 361 LNDHGNVLSEMEIDTQLIQRNDIVKIVPGAKVPVDGIVISGQSHVNESTITGEAKSILKT 420

Query: 421 PGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPA 480
           PGDKV+ GTVNENGCLFVK THVG DTTLS+IVQLVESAQLSRA AQKLAD+ISKFFVP 
Sbjct: 421 PGDKVIGGTVNENGCLFVKTTHVGLDTTLSRIVQLVESAQLSRAPAQKLADRISKFFVPV 480

Query: 481 VVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAV 540
           VVV A +TWLGWL  GEAG+YPK+WIPKGMDEFELALQFGISVLVIACPCALGLATPTA+
Sbjct: 481 VVVAASVTWLGWLFWGEAGLYPKYWIPKGMDEFELALQFGISVLVIACPCALGLATPTAI 540

Query: 541 MVASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCH 600
           MVASGKGASLGVLIKGASAL+NAYKVKTVVFDKTGTLT+GRP+VVSVVLFS F M  LC 
Sbjct: 541 MVASGKGASLGVLIKGASALQNAYKVKTVVFDKTGTLTVGRPEVVSVVLFSTFPMEVLCD 600

Query: 601 VAMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLAL 660
           VA+A+ESNS+HPLA +VV+HAKK+++K+G   ER   VENF VFPG GVGGKVD K   L
Sbjct: 601 VAIAVESNSEHPLAKSVVEHAKKMRKKFGSRTERLKRVENFEVFPGAGVGGKVD-KIPVL 660

Query: 661 VGNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVIS 720
           VGNRRL++A+NI +G +VDRY+ +NE  AQTCILVAINGRVAGGFGV DPPKPG +AVIS
Sbjct: 661 VGNRRLLQAHNIAVGPQVDRYVVENERLAQTCILVAINGRVAGGFGVTDPPKPGTRAVIS 720

Query: 721 YLRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGD 780
           YLRS GI+SIMVTGDN  TA A+AR +GI++V +E+DP GKANKIK LKMTG+IVAMVGD
Sbjct: 721 YLRSIGITSIMVTGDNWATAFAVARGVGINEVFAEMDPTGKANKIKTLKMTGNIVAMVGD 780

Query: 781 GVNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIW 840
           GVNDS ALAA+D+GIAIGAGT+IAIEAADIVLMRSN+EDVVTAIDLSRQT YRIW NYIW
Sbjct: 781 GVNDSHALAAADVGIAIGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIW 840

Query: 841 ALGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQS 897
           ALGYNII +PIAAGILYPF G RLPPWLAGACMAASSVSVVCSSL+LKC+KRPLNFQS
Sbjct: 841 ALGYNIIGMPIAAGILYPFIGIRLPPWLAGACMAASSVSVVCSSLVLKCYKRPLNFQS 889

BLAST of MC02g0093 vs. NCBI nr
Match: KAG6605210.1 (Copper-transporting ATPase HMA4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1273 bits (3294), Expect = 0.0
Identity = 658/898 (73.27%), Postives = 760/898 (84.63%), Query Frame = 0

Query: 1   MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES 60
           MD  +++L+ PLNP+  D+  R    +EAG F    + E+ IA CRLRI+ M CT+ A S
Sbjct: 1   MDVSINDLKVPLNPLDLDNEDRVAEFLEAGGFAAEEFSERGIAICRLRIRKMVCTAKARS 60

Query: 61  IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV 120
           +++A+ MV+GVKR ++G  I EAK+ FD +L  E  +L A DD+GF+ ELI++ADE  KV
Sbjct: 61  LQHAVRMVNGVKRVLIGPGIDEAKIFFDPNLTTEAEILAATDDSGFESELISAADEAYKV 120

Query: 121 HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ 180
           H+KLD+V   DM  IRTSLE A GVN VEMD   Q V+I Y+PD+TGPR L+QC      
Sbjct: 121 HIKLDQVGPADMTAIRTSLEQASGVNSVEMDGLGQMVSIHYDPDRTGPRSLLQC------ 180

Query: 181 GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLL-MVLPMLPPYGEWLDY 240
            L++Y ASLY+PP+RRD+EQ +E  T+RNLFL SCLFSVPVV   MVLPMLPPYGEWL++
Sbjct: 181 -LKSYGASLYVPPKRRDVEQHQEACTYRNLFLFSCLFSVPVVAFAMVLPMLPPYGEWLNF 240

Query: 241 MVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVY 300
            VYNMLTVGM+LKWI CTPVQF  G RFYVGSY AL+QKSANMDVLVA+GTNAAYFYSVY
Sbjct: 241 RVYNMLTVGMVLKWIFCTPVQFFAGRRFYVGSYRALQQKSANMDVLVAVGTNAAYFYSVY 300

Query: 301 IVIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLT 360
           IV KA  S  F+G+DFFE S+MLISFI LGKYLEV+AKGK+SDALAKLA LAP T+CL+T
Sbjct: 301 IVFKASTSNSFRGKDFFEFSSMLISFIQLGKYLEVMAKGKSSDALAKLAHLAPHTACLMT 360

Query: 361 LDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKK 420
            DD+ N+L+E+EIDTQ++QRND+IKIV GAKVPVDGIVI G+S+VNESTITGE+R   K 
Sbjct: 361 FDDHGNLLSEMEIDTQLIQRNDIIKIVAGAKVPVDGIVISGESNVNESTITGEARSIAKT 420

Query: 421 PGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPA 480
           PGDKV+ GTVNENGCLF+K THVG+DTTLS+IVQLVESAQLSRA AQKLAD+ISKFFVP 
Sbjct: 421 PGDKVIGGTVNENGCLFIKTTHVGSDTTLSRIVQLVESAQLSRAPAQKLADRISKFFVPV 480

Query: 481 VVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAV 540
           VVV AF+TWLGWLICGEAG+YPKH+IPKGMDEFELALQFGISVLVIACPCALGLATPTA+
Sbjct: 481 VVVAAFVTWLGWLICGEAGLYPKHFIPKGMDEFELALQFGISVLVIACPCALGLATPTAI 540

Query: 541 MVASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCH 600
           MVASGKGASLGVLIKGASAL+NAYKVK VVFDKTGTLT+GRP+VVSVVLFS F M ELC 
Sbjct: 541 MVASGKGASLGVLIKGASALQNAYKVKMVVFDKTGTLTVGRPEVVSVVLFSTFPMQELCD 600

Query: 601 VAMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLAL 660
           VA+AIESNSKHPLA  VV HA K+++K+G   E    ++NF VFPG GVGGK+D K + L
Sbjct: 601 VAIAIESNSKHPLAKCVVDHAMKMRKKFGARTECRKRIQNFEVFPGGGVGGKIDRKPV-L 660

Query: 661 VGNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVIS 720
           VGNRRLMRA+NI +G +VDRY+ +NE  AQTCILVAINGRVAGGFGV D PK G KAVIS
Sbjct: 661 VGNRRLMRAHNIVVGPQVDRYVIENERLAQTCILVAINGRVAGGFGVIDAPKSGTKAVIS 720

Query: 721 YLRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGD 780
           YLRS GISSIMVTGDN  TA A+AR +GI++V +E+DPIGKANKIK+LK TG+IVAMVGD
Sbjct: 721 YLRSIGISSIMVTGDNWATAFAVARGVGINEVFAEMDPIGKANKIKSLKTTGNIVAMVGD 780

Query: 781 GVNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIW 840
           GVNDS ALAA+D+GIAIGAGT+IAIEAADIVLMRSN+EDVVTAIDLSRQT YRIW NYIW
Sbjct: 781 GVNDSHALAAADVGIAIGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIW 840

Query: 841 ALGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQS 897
           ALGYNII +PIAAGILYPFTG RLPPWLAGACMAASS+SVVCSSLLLKC++RPLNFQS
Sbjct: 841 ALGYNIIGMPIAAGILYPFTGIRLPPWLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 890

BLAST of MC02g0093 vs. NCBI nr
Match: XP_022947845.1 (copper-transporting ATPase HMA4-like [Cucurbita moschata])

HSP 1 Score: 1266 bits (3276), Expect = 0.0
Identity = 659/913 (72.18%), Postives = 761/913 (83.35%), Query Frame = 0

Query: 1   MDSDLDELRAPLNPVHPDDGS---------------RTLRIIEAGNFDVNGYLEQDIATC 60
           MD  +++L+ PLNP+  D+ +               R    +EAG F    + EQ IA C
Sbjct: 1   MDGSINDLKVPLNPLDLDNNNNVSDHKYVVGISQEDRVAEFLEAGGFAAEEFSEQVIAIC 60

Query: 61  RLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAG 120
           RLRI+ M CT+ A S+++A+ MV+GVKR ++G  I EAK+ FD +L NE  +L A DD+G
Sbjct: 61  RLRIRRMVCTAKARSLQHAVRMVNGVKRVLIGPGIDEAKIFFDPNLTNEAEILAATDDSG 120

Query: 121 FDVELINSADEGNKVHVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQ 180
           F+ ELI++ADE  KVH+KLD+V   DM  IRTSLE A GVN VEMD   Q V+I Y+PD+
Sbjct: 121 FESELISAADEAYKVHIKLDQVGPADMTAIRTSLEQASGVNSVEMDGLGQMVSIHYDPDR 180

Query: 181 TGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLL- 240
           TGPR L+QC       L++Y ASLY+PP+RRD+EQ +E  T+RNLFL SCLFSVPVV   
Sbjct: 181 TGPRSLLQC-------LKSYGASLYVPPKRRDVEQHQEACTYRNLFLFSCLFSVPVVAFA 240

Query: 241 MVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDV 300
           MVLPMLPPYGEWL++ VYNMLTVGM+LKWI CTPVQF  G RFYVGSY AL+QKSANMDV
Sbjct: 241 MVLPMLPPYGEWLNFRVYNMLTVGMVLKWIFCTPVQFFAGRRFYVGSYRALQQKSANMDV 300

Query: 301 LVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDAL 360
           LVA+GTNAAYFYSVYIV KA  S  F+G+DFFE S+MLISFI LGKYLEV+AKGK+SDAL
Sbjct: 301 LVAVGTNAAYFYSVYIVFKASTSNSFRGKDFFEFSSMLISFIQLGKYLEVMAKGKSSDAL 360

Query: 361 AKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHV 420
           AKLA LAP  +CL+T DD+ N+L+E+EIDTQ++QRND+IKIV GAKVPVDGIVI G+S+V
Sbjct: 361 AKLAHLAPHAACLMTFDDHGNLLSEMEIDTQLIQRNDIIKIVAGAKVPVDGIVISGESNV 420

Query: 421 NESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRAS 480
           NESTITGE+R   K PGDKV+ GTVNENGCLF+K THVG+DTTLS+IVQLVESAQLSRA 
Sbjct: 421 NESTITGEARSIAKTPGDKVIGGTVNENGCLFIKTTHVGSDTTLSRIVQLVESAQLSRAP 480

Query: 481 AQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLV 540
           AQKLAD+ISKFFVP VVV AF+TWLGWLICGEAG+YPKH+IPKGMDEFELALQFGISVLV
Sbjct: 481 AQKLADRISKFFVPVVVVAAFVTWLGWLICGEAGLYPKHFIPKGMDEFELALQFGISVLV 540

Query: 541 IACPCALGLATPTAVMVASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVV 600
           IACPCALGLATPTA+MVASGKGASLGVLIKGASAL+NAYKVK VVFDKTGTLT+GRP+VV
Sbjct: 541 IACPCALGLATPTAIMVASGKGASLGVLIKGASALQNAYKVKMVVFDKTGTLTVGRPEVV 600

Query: 601 SVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFP 660
           SVVLFS F M ELC VA+AIESNSKHPLA  VV HA K+++K+G   E    ++NF VFP
Sbjct: 601 SVVLFSTFPMQELCDVAIAIESNSKHPLAKCVVDHAMKMRKKFGARTECRKRIQNFEVFP 660

Query: 661 GTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGF 720
           G GVGGK+D K + LVGNRRLMRA+NI +G +VDRY+ +NE  AQTCILVAINGRVAGGF
Sbjct: 661 GGGVGGKIDRKPV-LVGNRRLMRAHNIVVGPQVDRYVIENERLAQTCILVAINGRVAGGF 720

Query: 721 GVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKI 780
           GV D PK G KAVISYLRS GISSIMVTGDN  TA A+AR +GI++V +E+DPIGKANKI
Sbjct: 721 GVIDAPKSGTKAVISYLRSIGISSIMVTGDNWATAFAVARGVGINEVFAEMDPIGKANKI 780

Query: 781 KALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAID 840
           K+LK TG+IVAMVGDGVNDS ALAA+D+GIAIGAGT+IAIEAADIVLMRSN+EDVVTAID
Sbjct: 781 KSLKTTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIEAADIVLMRSNLEDVVTAID 840

Query: 841 LSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSL 897
           LSRQT YRIW NYIWALGYNII +PIAAGILYPFTG RLPPWLAGACMAASS+SVVCSSL
Sbjct: 841 LSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFTGIRLPPWLAGACMAASSLSVVCSSL 900

BLAST of MC02g0093 vs. NCBI nr
Match: XP_023007109.1 (copper-transporting ATPase HMA4-like, partial [Cucurbita maxima])

HSP 1 Score: 1259 bits (3258), Expect = 0.0
Identity = 650/877 (74.12%), Postives = 746/877 (85.06%), Query Frame = 0

Query: 22  RTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIG 81
           R    +EAG F    + EQ IA CRLRI+ M CT+ A S+++A+ MV+GVKR ++G  I 
Sbjct: 20  RVAEFLEAGGFAAEEFSEQGIAICRLRIRRMVCTAKARSLQHAVRMVNGVKRVLIGPGID 79

Query: 82  EAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKVHVKLDEVNFEDMITIRTSLEN 141
           EAK+ FD +L  E  +L A DD+GF+ ELI++ADE  KVH+KLD+V   DM  IRTSLE 
Sbjct: 80  EAKIFFDPNLTTEAEILAATDDSGFESELISAADEAYKVHIKLDQVGPADMTAIRTSLEQ 139

Query: 142 AVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQL 201
           A GVN VEMD   Q V+I Y+PD+TGPR L+QC       L++Y ASLY+PP+RRD+EQL
Sbjct: 140 ASGVNSVEMDGPGQMVSIHYDPDRTGPRSLLQC-------LKSYGASLYVPPKRRDVEQL 199

Query: 202 EEIRTHRNLFLLSCLFSVPVVLL-MVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQ 261
           +E  T+RNLFL SCLFSVPVV   MVLPMLPPYGEWL++ VYNMLTVGM+LKWI CTPVQ
Sbjct: 200 QEACTYRNLFLFSCLFSVPVVAFAMVLPMLPPYGEWLNFRVYNMLTVGMVLKWIFCTPVQ 259

Query: 262 FIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSA 321
           F  G RFYVGSY AL+QKSANMDVLVA+GTNAAYFYSVYIV KA  S  F+G+DFFE S+
Sbjct: 260 FFAGRRFYVGSYRALQQKSANMDVLVAVGTNAAYFYSVYIVFKASTSNSFRGKDFFEFSS 319

Query: 322 MLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRN 381
           MLISFI LGKYLEV+AKGK+SD LAKLA LAP T+CL+T DD+ N+L+E+EIDTQ++QRN
Sbjct: 320 MLISFIQLGKYLEVMAKGKSSDTLAKLAHLAPHTACLMTFDDHGNLLSEMEIDTQLIQRN 379

Query: 382 DVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKAT 441
           D+IK+V GAKVPVDGIVI G+S+VNESTITGE+R   K PGDKV+ GTVNENGCLF+K T
Sbjct: 380 DIIKVVAGAKVPVDGIVISGESNVNESTITGEARSIAKTPGDKVIGGTVNENGCLFIKTT 439

Query: 442 HVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIY 501
           HVG+DTTLS+IVQLVESAQLSRA AQKLAD+ISKFFVP VVV AF+TWLGWLICGEAG+Y
Sbjct: 440 HVGSDTTLSRIVQLVESAQLSRAPAQKLADRISKFFVPVVVVAAFVTWLGWLICGEAGLY 499

Query: 502 PKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASALE 561
           PKH+IPKGMDEFELALQFGISVLVIACPCALGLATPTA+MVASGKGASLGVLIKGASAL+
Sbjct: 500 PKHFIPKGMDEFELALQFGISVLVIACPCALGLATPTAIMVASGKGASLGVLIKGASALQ 559

Query: 562 NAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHA 621
           NAYKVK VVFDKTGTLT+GRP+VVSVVLFS F M ELC VA+AIESNSKHPLA  VV HA
Sbjct: 560 NAYKVKMVVFDKTGTLTVGRPEVVSVVLFSTFPMQELCDVAIAIESNSKHPLAKCVVDHA 619

Query: 622 KKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDRY 681
            K+++K+G   E    ++NF VFPG GVGGK+D K + LVGNRRLMRA+NI +G +VDRY
Sbjct: 620 MKMRKKFGARTECRKRIQNFEVFPGGGVGGKIDRKPV-LVGNRRLMRAHNIVVGPQVDRY 679

Query: 682 IADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTAA 741
           + +NE  AQTCILVAINGRVAGGFGV D PK G KAVISYLRS GISSIMVTGDN  TA 
Sbjct: 680 VIENERLAQTCILVAINGRVAGGFGVIDAPKSGTKAVISYLRSIGISSIMVTGDNWATAF 739

Query: 742 ALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAGT 801
           A+AR +GI++V +E+DPIGKANKIK+LK TG+IVAMVGDGVNDS ALAA+D+GIAIGAGT
Sbjct: 740 AVARGVGINEVFAEMDPIGKANKIKSLKTTGNIVAMVGDGVNDSHALAAADVGIAIGAGT 799

Query: 802 DIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFTG 861
           +IAIEAADIVLMRSN+EDVVTAIDLSRQT YRIW NYIWALGYNII +PIAAGILYPFTG
Sbjct: 800 NIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFTG 859

Query: 862 TRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQS 897
            RLPPWLAGACMAASS+SVVCSSLLLKC++RPLNFQS
Sbjct: 860 IRLPPWLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 888

BLAST of MC02g0093 vs. ExPASy TrEMBL
Match: A0A6J1D664 (copper-transporting ATPase HMA4-like OS=Momordica charantia OX=3673 GN=LOC111017415 PE=3 SV=1)

HSP 1 Score: 1745 bits (4520), Expect = 0.0
Identity = 899/899 (100.00%), Postives = 899/899 (100.00%), Query Frame = 0

Query: 1   MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES 60
           MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES
Sbjct: 1   MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES 60

Query: 61  IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV 120
           IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV
Sbjct: 61  IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV 120

Query: 121 HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ 180
           HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ
Sbjct: 121 HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ 180

Query: 181 GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLLMVLPMLPPYGEWLDYM 240
           GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLLMVLPMLPPYGEWLDYM
Sbjct: 181 GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLLMVLPMLPPYGEWLDYM 240

Query: 241 VYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYI 300
           VYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYI
Sbjct: 241 VYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYI 300

Query: 301 VIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTL 360
           VIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTL
Sbjct: 301 VIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTL 360

Query: 361 DDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKP 420
           DDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKP
Sbjct: 361 DDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKP 420

Query: 421 GDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAV 480
           GDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAV
Sbjct: 421 GDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAV 480

Query: 481 VVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVM 540
           VVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVM
Sbjct: 481 VVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVM 540

Query: 541 VASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHV 600
           VASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHV
Sbjct: 541 VASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHV 600

Query: 601 AMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALV 660
           AMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALV
Sbjct: 601 AMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALV 660

Query: 661 GNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISY 720
           GNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISY
Sbjct: 661 GNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISY 720

Query: 721 LRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDG 780
           LRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDG
Sbjct: 721 LRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDG 780

Query: 781 VNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWA 840
           VNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWA
Sbjct: 781 VNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWA 840

Query: 841 LGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQSII 899
           LGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQSII
Sbjct: 841 LGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQSII 899

BLAST of MC02g0093 vs. ExPASy TrEMBL
Match: A0A6J1D6X6 (copper-transporting ATPase HMA4-like OS=Momordica charantia OX=3673 GN=LOC111017908 PE=3 SV=1)

HSP 1 Score: 1298 bits (3359), Expect = 0.0
Identity = 664/898 (73.94%), Postives = 772/898 (85.97%), Query Frame = 0

Query: 1   MDSDLDELRAPLNPVHPDDGSRTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAES 60
           M++ LD+L+ PLNP   ++ +R + II+AG F  + +L QD+  CRL+I+ MT  + A+S
Sbjct: 1   METKLDDLKIPLNPTTLNNENRAVEIIDAGGFPADEFL-QDVEICRLKIRSMTFRANAQS 60

Query: 61  IENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKV 120
           ++NAL  V GVKR V+   I EA V FD +  NE++++EA+  +GF+VEL+  AD   KV
Sbjct: 61  LQNALTTVHGVKRTVIAPEIEEATVHFDPNSTNEEQIIEAISGSGFEVELLTFADNPYKV 120

Query: 121 HVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQ 180
           H+KL+ V+FEDM  IR+SLE A GVN VEMD   QKV I Y+PDQTGPR L+QC      
Sbjct: 121 HIKLERVSFEDMAAIRSSLEQACGVNFVEMDVVGQKVAIGYDPDQTGPRSLLQC------ 180

Query: 181 GLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLL-MVLPMLPPYGEWLDY 240
            L++Y ASLY+PPRRRD++QL+E+ T+RNLFLLSCLFSVPVV   MVLPMLPPYG+WL+Y
Sbjct: 181 -LKSYRASLYVPPRRRDVQQLQEVCTYRNLFLLSCLFSVPVVAFAMVLPMLPPYGDWLNY 240

Query: 241 MVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVY 300
             YNMLTVGM+L+WI CTPVQFI G RFYVGSY+ALRQKSANMDVLVALGTNAAYFYSVY
Sbjct: 241 RAYNMLTVGMVLRWIFCTPVQFIAGRRFYVGSYHALRQKSANMDVLVALGTNAAYFYSVY 300

Query: 301 IVIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLT 360
           I+ KAF S  F+G+DFFETS+MLISFILLGKYLEV+AKGKTSDALAKLA LAPDT+CL+T
Sbjct: 301 ILFKAFTSTSFRGQDFFETSSMLISFILLGKYLEVMAKGKTSDALAKLAHLAPDTACLMT 360

Query: 361 LDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKK 420
           L+D+ N+L+E+EIDTQ++QRND++KIVPGAKVPVDGIVI GQSHVNESTITGE++  +K 
Sbjct: 361 LNDHGNVLSEMEIDTQLIQRNDIVKIVPGAKVPVDGIVISGQSHVNESTITGEAKSILKT 420

Query: 421 PGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPA 480
           PGDKV+ GTVNENGCLFVK THVG DTTLS+IVQLVESAQLSRA AQKLAD+ISKFFVP 
Sbjct: 421 PGDKVIGGTVNENGCLFVKTTHVGLDTTLSRIVQLVESAQLSRAPAQKLADRISKFFVPV 480

Query: 481 VVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAV 540
           VVV A +TWLGWL  GEAG+YPK+WIPKGMDEFELALQFGISVLVIACPCALGLATPTA+
Sbjct: 481 VVVAASVTWLGWLFWGEAGLYPKYWIPKGMDEFELALQFGISVLVIACPCALGLATPTAI 540

Query: 541 MVASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCH 600
           MVASGKGASLGVLIKGASAL+NAYKVKTVVFDKTGTLT+GRP+VVSVVLFS F M  LC 
Sbjct: 541 MVASGKGASLGVLIKGASALQNAYKVKTVVFDKTGTLTVGRPEVVSVVLFSTFPMEVLCD 600

Query: 601 VAMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLAL 660
           VA+A+ESNS+HPLA +VV+HAKK+++K+G   ER   VENF VFPG GVGGKVD K   L
Sbjct: 601 VAIAVESNSEHPLAKSVVEHAKKMRKKFGSRTERLKRVENFEVFPGAGVGGKVD-KIPVL 660

Query: 661 VGNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVIS 720
           VGNRRL++A+NI +G +VDRY+ +NE  AQTCILVAINGRVAGGFGV DPPKPG +AVIS
Sbjct: 661 VGNRRLLQAHNIAVGPQVDRYVVENERLAQTCILVAINGRVAGGFGVTDPPKPGTRAVIS 720

Query: 721 YLRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGD 780
           YLRS GI+SIMVTGDN  TA A+AR +GI++V +E+DP GKANKIK LKMTG+IVAMVGD
Sbjct: 721 YLRSIGITSIMVTGDNWATAFAVARGVGINEVFAEMDPTGKANKIKTLKMTGNIVAMVGD 780

Query: 781 GVNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIW 840
           GVNDS ALAA+D+GIAIGAGT+IAIEAADIVLMRSN+EDVVTAIDLSRQT YRIW NYIW
Sbjct: 781 GVNDSHALAAADVGIAIGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIW 840

Query: 841 ALGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQS 897
           ALGYNII +PIAAGILYPF G RLPPWLAGACMAASSVSVVCSSL+LKC+KRPLNFQS
Sbjct: 841 ALGYNIIGMPIAAGILYPFIGIRLPPWLAGACMAASSVSVVCSSLVLKCYKRPLNFQS 889

BLAST of MC02g0093 vs. ExPASy TrEMBL
Match: A0A6J1G814 (copper-transporting ATPase HMA4-like OS=Cucurbita moschata OX=3662 GN=LOC111451605 PE=3 SV=1)

HSP 1 Score: 1266 bits (3276), Expect = 0.0
Identity = 659/913 (72.18%), Postives = 761/913 (83.35%), Query Frame = 0

Query: 1   MDSDLDELRAPLNPVHPDDGS---------------RTLRIIEAGNFDVNGYLEQDIATC 60
           MD  +++L+ PLNP+  D+ +               R    +EAG F    + EQ IA C
Sbjct: 1   MDGSINDLKVPLNPLDLDNNNNVSDHKYVVGISQEDRVAEFLEAGGFAAEEFSEQVIAIC 60

Query: 61  RLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVLEAVDDAG 120
           RLRI+ M CT+ A S+++A+ MV+GVKR ++G  I EAK+ FD +L NE  +L A DD+G
Sbjct: 61  RLRIRRMVCTAKARSLQHAVRMVNGVKRVLIGPGIDEAKIFFDPNLTNEAEILAATDDSG 120

Query: 121 FDVELINSADEGNKVHVKLDEVNFEDMITIRTSLENAVGVNLVEMDFSQQKVTISYEPDQ 180
           F+ ELI++ADE  KVH+KLD+V   DM  IRTSLE A GVN VEMD   Q V+I Y+PD+
Sbjct: 121 FESELISAADEAYKVHIKLDQVGPADMTAIRTSLEQASGVNSVEMDGLGQMVSIHYDPDR 180

Query: 181 TGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSCLFSVPVVLL- 240
           TGPR L+QC       L++Y ASLY+PP+RRD+EQ +E  T+RNLFL SCLFSVPVV   
Sbjct: 181 TGPRSLLQC-------LKSYGASLYVPPKRRDVEQHQEACTYRNLFLFSCLFSVPVVAFA 240

Query: 241 MVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYALRQKSANMDV 300
           MVLPMLPPYGEWL++ VYNMLTVGM+LKWI CTPVQF  G RFYVGSY AL+QKSANMDV
Sbjct: 241 MVLPMLPPYGEWLNFRVYNMLTVGMVLKWIFCTPVQFFAGRRFYVGSYRALQQKSANMDV 300

Query: 301 LVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLEVIAKGKTSDAL 360
           LVA+GTNAAYFYSVYIV KA  S  F+G+DFFE S+MLISFI LGKYLEV+AKGK+SDAL
Sbjct: 301 LVAVGTNAAYFYSVYIVFKASTSNSFRGKDFFEFSSMLISFIQLGKYLEVMAKGKSSDAL 360

Query: 361 AKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVDGIVIHGQSHV 420
           AKLA LAP  +CL+T DD+ N+L+E+EIDTQ++QRND+IKIV GAKVPVDGIVI G+S+V
Sbjct: 361 AKLAHLAPHAACLMTFDDHGNLLSEMEIDTQLIQRNDIIKIVAGAKVPVDGIVISGESNV 420

Query: 421 NESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQLVESAQLSRAS 480
           NESTITGE+R   K PGDKV+ GTVNENGCLF+K THVG+DTTLS+IVQLVESAQLSRA 
Sbjct: 421 NESTITGEARSIAKTPGDKVIGGTVNENGCLFIKTTHVGSDTTLSRIVQLVESAQLSRAP 480

Query: 481 AQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFELALQFGISVLV 540
           AQKLAD+ISKFFVP VVV AF+TWLGWLICGEAG+YPKH+IPKGMDEFELALQFGISVLV
Sbjct: 481 AQKLADRISKFFVPVVVVAAFVTWLGWLICGEAGLYPKHFIPKGMDEFELALQFGISVLV 540

Query: 541 IACPCALGLATPTAVMVASGKGASLGVLIKGASALENAYKVKTVVFDKTGTLTLGRPKVV 600
           IACPCALGLATPTA+MVASGKGASLGVLIKGASAL+NAYKVK VVFDKTGTLT+GRP+VV
Sbjct: 541 IACPCALGLATPTAIMVASGKGASLGVLIKGASALQNAYKVKMVVFDKTGTLTVGRPEVV 600

Query: 601 SVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFP 660
           SVVLFS F M ELC VA+AIESNSKHPLA  VV HA K+++K+G   E    ++NF VFP
Sbjct: 601 SVVLFSTFPMQELCDVAIAIESNSKHPLAKCVVDHAMKMRKKFGARTECRKRIQNFEVFP 660

Query: 661 GTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDRYIADNEHKAQTCILVAINGRVAGGF 720
           G GVGGK+D K + LVGNRRLMRA+NI +G +VDRY+ +NE  AQTCILVAINGRVAGGF
Sbjct: 661 GGGVGGKIDRKPV-LVGNRRLMRAHNIVVGPQVDRYVIENERLAQTCILVAINGRVAGGF 720

Query: 721 GVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTAAALARDLGIDDVLSEVDPIGKANKI 780
           GV D PK G KAVISYLRS GISSIMVTGDN  TA A+AR +GI++V +E+DPIGKANKI
Sbjct: 721 GVIDAPKSGTKAVISYLRSIGISSIMVTGDNWATAFAVARGVGINEVFAEMDPIGKANKI 780

Query: 781 KALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAID 840
           K+LK TG+IVAMVGDGVNDS ALAA+D+GIAIGAGT+IAIEAADIVLMRSN+EDVVTAID
Sbjct: 781 KSLKTTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIEAADIVLMRSNLEDVVTAID 840

Query: 841 LSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSL 897
           LSRQT YRIW NYIWALGYNII +PIAAGILYPFTG RLPPWLAGACMAASS+SVVCSSL
Sbjct: 841 LSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFTGIRLPPWLAGACMAASSLSVVCSSL 900

BLAST of MC02g0093 vs. ExPASy TrEMBL
Match: A0A6J1L423 (copper-transporting ATPase HMA4-like OS=Cucurbita maxima OX=3661 GN=LOC111499707 PE=3 SV=1)

HSP 1 Score: 1259 bits (3258), Expect = 0.0
Identity = 650/877 (74.12%), Postives = 746/877 (85.06%), Query Frame = 0

Query: 22  RTLRIIEAGNFDVNGYLEQDIATCRLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIG 81
           R    +EAG F    + EQ IA CRLRI+ M CT+ A S+++A+ MV+GVKR ++G  I 
Sbjct: 20  RVAEFLEAGGFAAEEFSEQGIAICRLRIRRMVCTAKARSLQHAVRMVNGVKRVLIGPGID 79

Query: 82  EAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKVHVKLDEVNFEDMITIRTSLEN 141
           EAK+ FD +L  E  +L A DD+GF+ ELI++ADE  KVH+KLD+V   DM  IRTSLE 
Sbjct: 80  EAKIFFDPNLTTEAEILAATDDSGFESELISAADEAYKVHIKLDQVGPADMTAIRTSLEQ 139

Query: 142 AVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQL 201
           A GVN VEMD   Q V+I Y+PD+TGPR L+QC       L++Y ASLY+PP+RRD+EQL
Sbjct: 140 ASGVNSVEMDGPGQMVSIHYDPDRTGPRSLLQC-------LKSYGASLYVPPKRRDVEQL 199

Query: 202 EEIRTHRNLFLLSCLFSVPVVLL-MVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQ 261
           +E  T+RNLFL SCLFSVPVV   MVLPMLPPYGEWL++ VYNMLTVGM+LKWI CTPVQ
Sbjct: 200 QEACTYRNLFLFSCLFSVPVVAFAMVLPMLPPYGEWLNFRVYNMLTVGMVLKWIFCTPVQ 259

Query: 262 FIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSA 321
           F  G RFYVGSY AL+QKSANMDVLVA+GTNAAYFYSVYIV KA  S  F+G+DFFE S+
Sbjct: 260 FFAGRRFYVGSYRALQQKSANMDVLVAVGTNAAYFYSVYIVFKASTSNSFRGKDFFEFSS 319

Query: 322 MLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRN 381
           MLISFI LGKYLEV+AKGK+SD LAKLA LAP T+CL+T DD+ N+L+E+EIDTQ++QRN
Sbjct: 320 MLISFIQLGKYLEVMAKGKSSDTLAKLAHLAPHTACLMTFDDHGNLLSEMEIDTQLIQRN 379

Query: 382 DVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKAT 441
           D+IK+V GAKVPVDGIVI G+S+VNESTITGE+R   K PGDKV+ GTVNENGCLF+K T
Sbjct: 380 DIIKVVAGAKVPVDGIVISGESNVNESTITGEARSIAKTPGDKVIGGTVNENGCLFIKTT 439

Query: 442 HVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIY 501
           HVG+DTTLS+IVQLVESAQLSRA AQKLAD+ISKFFVP VVV AF+TWLGWLICGEAG+Y
Sbjct: 440 HVGSDTTLSRIVQLVESAQLSRAPAQKLADRISKFFVPVVVVAAFVTWLGWLICGEAGLY 499

Query: 502 PKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASALE 561
           PKH+IPKGMDEFELALQFGISVLVIACPCALGLATPTA+MVASGKGASLGVLIKGASAL+
Sbjct: 500 PKHFIPKGMDEFELALQFGISVLVIACPCALGLATPTAIMVASGKGASLGVLIKGASALQ 559

Query: 562 NAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHA 621
           NAYKVK VVFDKTGTLT+GRP+VVSVVLFS F M ELC VA+AIESNSKHPLA  VV HA
Sbjct: 560 NAYKVKMVVFDKTGTLTVGRPEVVSVVLFSTFPMQELCDVAIAIESNSKHPLAKCVVDHA 619

Query: 622 KKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDRY 681
            K+++K+G   E    ++NF VFPG GVGGK+D K + LVGNRRLMRA+NI +G +VDRY
Sbjct: 620 MKMRKKFGARTECRKRIQNFEVFPGGGVGGKIDRKPV-LVGNRRLMRAHNIVVGPQVDRY 679

Query: 682 IADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTAA 741
           + +NE  AQTCILVAINGRVAGGFGV D PK G KAVISYLRS GISSIMVTGDN  TA 
Sbjct: 680 VIENERLAQTCILVAINGRVAGGFGVIDAPKSGTKAVISYLRSIGISSIMVTGDNWATAF 739

Query: 742 ALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAGT 801
           A+AR +GI++V +E+DPIGKANKIK+LK TG+IVAMVGDGVNDS ALAA+D+GIAIGAGT
Sbjct: 740 AVARGVGINEVFAEMDPIGKANKIKSLKTTGNIVAMVGDGVNDSHALAAADVGIAIGAGT 799

Query: 802 DIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFTG 861
           +IAIEAADIVLMRSN+EDVVTAIDLSRQT YRIW NYIWALGYNII +PIAAGILYPFTG
Sbjct: 800 NIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFTG 859

Query: 862 TRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQS 897
            RLPPWLAGACMAASS+SVVCSSLLLKC++RPLNFQS
Sbjct: 860 IRLPPWLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 888

BLAST of MC02g0093 vs. ExPASy TrEMBL
Match: A0A0A0LLU8 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G279210 PE=3 SV=1)

HSP 1 Score: 1199 bits (3102), Expect = 0.0
Identity = 620/878 (70.62%), Postives = 729/878 (83.03%), Query Frame = 0

Query: 22  RTLRIIEAGNFDVNGYLEQDI-ATCRLRIKGMTCTSCAESIENALMMVDGVKRAVVGITI 81
           RT+  IEAG F  + +  Q I +TCRLRI  +T  +   S++  L MV GVKRA V +  
Sbjct: 47  RTIDTIEAGGFLTDEFFNQAITSTCRLRISQITYPAKLRSLQKGLAMVHGVKRATVHLEF 106

Query: 82  GEAKVLFDSSLINEDRVLEAVDDAGFDVELINSADEGNKVHVKLDEVNFEDMITIRTSLE 141
            EAKVLFD +L  E  +L+A+ D GF+ +LI+  DE  +VH+KLD  +  DM  I++SLE
Sbjct: 107 KEAKVLFDPNLTTETLILKAIADIGFEADLISVGDEAYEVHLKLDRASSGDMGAIKSSLE 166

Query: 142 NAVGVNLVEMDFSQQKVTISYEPDQTGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQ 201
            AVGV  VEM+  ++ VT+ YEPD+TGPR ++Q +E+       Y A LY+PP+RR++EQ
Sbjct: 167 QAVGVTYVEMEVVERMVTVGYEPDRTGPRSILQFLEK-------YGARLYVPPKRRELEQ 226

Query: 202 LEEIRTHRNLFLLSCLFSVPVVLL-MVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPV 261
            +E   +RNLFL SCLFSVPVV   MVLPMLPPYG+WL++ V  MLT+GM+LKWI CTPV
Sbjct: 227 HQEACAYRNLFLFSCLFSVPVVAFAMVLPMLPPYGDWLNFRVCKMLTIGMVLKWIFCTPV 286

Query: 262 QFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETS 321
           QF+ G RFYVGSY AL++KSANMDVLVA+GTNAAYFYSVYIV KA  S  F+G+DFFETS
Sbjct: 287 QFLAGGRFYVGSYRALQRKSANMDVLVAVGTNAAYFYSVYIVFKASTSNSFRGKDFFETS 346

Query: 322 AMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQR 381
           +MLISFILLGKYLEV+AKGK+SDAL KLA LAPDT+CL+  DDN ++L+EVEIDTQ++QR
Sbjct: 347 SMLISFILLGKYLEVMAKGKSSDALGKLAHLAPDTACLMNFDDNGSLLSEVEIDTQLIQR 406

Query: 382 NDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKA 441
           ND+IKIVPGAKVPVDGIVI G+S+VNESTITGE+R   K  GDKV+ GTVNENG LFVK 
Sbjct: 407 NDIIKIVPGAKVPVDGIVIGGESNVNESTITGEARSICKSTGDKVIGGTVNENGILFVKT 466

Query: 442 THVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGI 501
           THVG DTTLS+IVQLVESAQLSRA AQKLADQISKFFVP VVV AF+TWLGWLICGE G+
Sbjct: 467 THVGTDTTLSRIVQLVESAQLSRAPAQKLADQISKFFVPVVVVAAFVTWLGWLICGEIGL 526

Query: 502 YPKHWIPKGMDEFELALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASAL 561
           YPKHWIPKGMDEFELALQF ISVLVIACPCALGLATPTA+MVASGKGASLGVLIKGASAL
Sbjct: 527 YPKHWIPKGMDEFELALQFSISVLVIACPCALGLATPTAIMVASGKGASLGVLIKGASAL 586

Query: 562 ENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQH 621
           +NAYKVKTVVFDKTGTLT+GRP+VVSVVLFS F M E+C  A+AIESNS HP A  VV+H
Sbjct: 587 QNAYKVKTVVFDKTGTLTVGRPEVVSVVLFSTFPMQEVCDAAIAIESNSDHPFAKPVVEH 646

Query: 622 AKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDR 681
           AKK+++K+G  +E    V+NF VFPG GVGGK+D K + LVGN+RLMR +N+ +  +VDR
Sbjct: 647 AKKMRRKFGARSECCKRVQNFEVFPGGGVGGKIDRKTV-LVGNKRLMRVHNVDITPQVDR 706

Query: 682 YIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTA 741
           Y  +NE  AQTC+LVAING++AGGFGV D PKPG KA+IS+LRS GIS+IM+TGDN  TA
Sbjct: 707 YTIENERLAQTCVLVAINGKIAGGFGVLDTPKPGTKAIISFLRSIGISTIMITGDNLATA 766

Query: 742 AALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAG 801
            A+AR +GI+ V +E+DPI KAN+IK+LK  G+IVAMVGDGVNDS ALAA+D+GIAIGAG
Sbjct: 767 FAVARGVGINKVFAEMDPIEKANQIKSLKTRGNIVAMVGDGVNDSHALAAADVGIAIGAG 826

Query: 802 TDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFT 861
           T+IAIEAADIVLMRSN+EDVVTAIDLSRQT YRIW NYIWALGYNII +PIAAGILYPF 
Sbjct: 827 TNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFF 886

Query: 862 GTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQS 897
           G RLPPWLAGACMAASS+SVVCSSLLLKC++RPLNFQS
Sbjct: 887 GIRLPPWLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 916

BLAST of MC02g0093 vs. TAIR 10
Match: AT1G63440.1 (heavy metal atpase 5 )

HSP 1 Score: 884.8 bits (2285), Expect = 5.8e-257
Identity = 480/860 (55.81%), Postives = 619/860 (71.98%), Query Frame = 0

Query: 39  EQDIATCRLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDRVL 98
           E+    CR+RI GMTCTSC+ +IE  L  V+GV+RA V + I EA++ +D  L + DR+L
Sbjct: 125 ERSRQVCRIRINGMTCTSCSSTIERVLQSVNGVQRAHVALAIEEAEIHYDPRLSSYDRLL 184

Query: 99  EAVDDAGFDVELINSADEGNKVHVKLD-EVNFEDMITIRTSLENAVGVNLVEMDFSQQKV 158
           E +++AGF+  LI++ ++ +K+ +K+D E+  E M  I  SLE   GV  VE+     K+
Sbjct: 185 EEIENAGFEAVLISTGEDVSKIDLKIDGELTDESMKVIERSLEALPGVQSVEISHGTDKI 244

Query: 159 TISYEPDQTGPRFLIQCIEEAAQGLRTY-NASLYLPPR-RRDIEQLEEIRTHRNLFLLSC 218
           ++ Y+PD TGPR  IQ IE    G   +  A+++      R+ ++  EI+ +   FL S 
Sbjct: 245 SVLYKPDVTGPRNFIQVIESTVFGHSGHIKATIFSEGGVGRESQKQGEIKQYYKSFLWSL 304

Query: 219 LFSVPVVL-LMVLPMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYA 278
           +F+VPV L  MV   +P   + L + V NMLTVG +++ +L TPVQF++GWRFY GSY A
Sbjct: 305 VFTVPVFLTAMVFMYIPGIKDLLMFKVINMLTVGEIIRCVLATPVQFVIGWRFYTGSYKA 364

Query: 279 LRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLEV 338
           LR+ SANMDVL+ALGTNAAYFYS+Y V++A  S  F+G DFFETSAMLISFI+LGKYLEV
Sbjct: 365 LRRGSANMDVLIALGTNAAYFYSLYTVLRAATSPDFKGVDFFETSAMLISFIILGKYLEV 424

Query: 339 IAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVD 398
           +AKGKTS A+AKL  LAPDT+ LL+LD   N+  E EID +++Q+NDVIKIVPGAKV  D
Sbjct: 425 MAKGKTSQAIAKLMNLAPDTAILLSLDKEGNVTGEEEIDGRLIQKNDVIKIVPGAKVASD 484

Query: 399 GIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQL 458
           G VI GQSHVNES ITGE+RP  K+ GD V+ GT+NENG L VK T VG+++ L+QIV+L
Sbjct: 485 GYVIWGQSHVNESMITGEARPVAKRKGDTVIGGTLNENGVLHVKVTRVGSESALAQIVRL 544

Query: 459 VESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFEL 518
           VESAQL++A  QKLAD+ISKFFVP V+ ++F TWL W + G+   YP+ WIP  MD FEL
Sbjct: 545 VESAQLAKAPVQKLADRISKFFVPLVIFLSFSTWLAWFLAGKLHWYPESWIPSSMDSFEL 604

Query: 519 ALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASALENAYKVKTVVFDKTG 578
           ALQFGISV+VIACPCALGLATPTAVMV +G GAS GVLIKG  ALE A+KV  +VFDKTG
Sbjct: 605 ALQFGISVMVIACPCALGLATPTAVMVGTGVGASQGVLIKGGQALERAHKVNCIVFDKTG 664

Query: 579 TLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHAKKVQQKYGFSAERP 638
           TLT+G+P VV   L  N  + E   +  A E NS+HPLA A+V++AKK +       E P
Sbjct: 665 TLTMGKPVVVKTKLLKNMVLREFYELVAATEVNSEHPLAKAIVEYAKKFRD----DEENP 724

Query: 639 GHVE--NFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVDRYIADNEHKAQTCI 698
              E  +F    G GV   V  + + +VGN+ LM  + + +  + +  +AD+E  AQT I
Sbjct: 725 AWPEACDFVSITGKGVKATVKGREI-MVGNKNLMNDHKVIIPDDAEELLADSEDMAQTGI 784

Query: 699 LVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFTAAALARDLGIDDVL 758
           LV+IN  + G   V+DP KP A+  IS L+S  I SIMVTGDN  TA ++AR++GID V+
Sbjct: 785 LVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSIMVTGDNWGTANSIAREVGIDSVI 844

Query: 759 SEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGAGTDIAIEAADIVLM 818
           +E  P  KA K+K L+  G +VAMVGDG+NDSPAL A+D+G+AIGAGTDIAIEAADIVLM
Sbjct: 845 AEAKPEQKAEKVKELQAAGHVVAMVGDGINDSPALVAADVGMAIGAGTDIAIEAADIVLM 904

Query: 819 RSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPFTGTRLPPWLAGACM 878
           +SN+EDV+TAIDLSR+T  RI  NY+WALGYN++ +PIAAG+L+P T  RLPPW+AGA M
Sbjct: 905 KSNLEDVITAIDLSRKTFSRIRLNYVWALGYNLMGIPIAAGVLFPGTRFRLPPWIAGAAM 964

Query: 879 AASSVSVVCSSLLLKCFKRP 893
           AASSVSVVC SLLLK +KRP
Sbjct: 965 AASSVSVVCCSLLLKNYKRP 979

BLAST of MC02g0093 vs. TAIR 10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1) )

HSP 1 Score: 698.0 bits (1800), Expect = 1.0e-200
Identity = 396/874 (45.31%), Postives = 558/874 (63.84%), Query Frame = 0

Query: 39  EQDIATC--RLRIKGMTCTSCAESIENALMMVDGVKRAVVGITIGEAKVLFDSSLINEDR 98
           EQ  AT   +  I GMTC +C  S+E  L  + GVKRAVV ++    +V +D ++IN+D 
Sbjct: 127 EQTQATLVGQFTIGGMTCAACVNSVEGILRDLPGVKRAVVALSTSLGEVEYDPNVINKDD 186

Query: 99  VLEAVDDAGFDVELINSADEGNKVHVKLDEV-NFEDMITIRTSLENAVGVNLVEMDFSQQ 158
           ++ A++DAGF+  L+ S ++ +K+ +++D + N  D   +   L    GV    +D    
Sbjct: 187 IVNAIEDAGFEGSLVQS-NQQDKLVLRVDGILNELDAQVLEGILTRLNGVRQFRLDRISG 246

Query: 159 KVTISYEPDQTGPRFLIQCIEEAAQGLRTYNASLYLPPRRRDIEQLEEIRTHRNLFLLSC 218
           ++ + ++P+    R L+  IEE   G   +   +  P  R   +   E       F+ S 
Sbjct: 247 ELEVVFDPEVVSSRSLVDGIEE--DGFGKFKLRVMSPYERLSSKDTGEASNMFRRFISSL 306

Query: 219 LFSVPVVLLMVL-PMLPPYGEWLDYMVYNMLTVGMLLKWILCTPVQFIVGWRFYVGSYYA 278
           + S+P+  + V+ P +  +   L +       +G  LKW L + +QF++G RFYV ++ A
Sbjct: 307 VLSIPLFFIQVICPHIALFDALLVWRC-GPFMMGDWLKWALVSVIQFVIGKRFYVAAWRA 366

Query: 279 LRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLEV 338
           LR  S NMDVLVALGT+A+YFYSV  ++   A   F    +F+ SAMLI+F+LLGKYLE 
Sbjct: 367 LRNGSTNMDVLVALGTSASYFYSVGALLYG-AVTGFWSPTYFDASAMLITFVLLGKYLES 426

Query: 339 IAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDTQMLQRNDVIKIVPGAKVPVD 398
           +AKGKTSDA+ KL +L P T+ LLT      ++ E EID  ++Q  D +K+ PGAK+P D
Sbjct: 427 LAKGKTSDAMKKLVQLTPATAILLTEGKGGKLVGEREIDALLIQPGDTLKVHPGAKIPAD 486

Query: 399 GIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGADTTLSQIVQL 458
           G+V+ G S+VNES +TGES P  K+    V+ GT+N +G L +KAT VG+D  LSQI+ L
Sbjct: 487 GVVVWGSSYVNESMVTGESVPVSKEVDSPVIGGTINMHGALHMKATKVGSDAVLSQIISL 546

Query: 459 VESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHWIPKGMDEFEL 518
           VE+AQ+S+A  QK AD ++  FVP V+ +A  T +GW I G  G YP  W+P+    F  
Sbjct: 547 VETAQMSKAPIQKFADYVASIFVPVVITLALFTLVGWSIGGAVGAYPDEWLPENGTHFVF 606

Query: 519 ALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASALENAYKVKTVVFDKTG 578
           +L F ISV+VIACPCALGLATPTAVMVA+G GA+ GVLIKG  ALE A+KVK V+FDKTG
Sbjct: 607 SLMFSISVVVIACPCALGLATPTAVMVATGVGATNGVLIKGGDALEKAHKVKYVIFDKTG 666

Query: 579 TLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQHAK------------- 638
           TLT G+  V +  +FS     E   +  + E++S+HPLA A+V +A+             
Sbjct: 667 TLTQGKATVTTTKVFSEMDRGEFLTLVASAEASSEHPLAKAIVAYARHFHFFDESTEDGE 726

Query: 639 ---KVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALVGNRRLMRANNIRMGTEVD 698
              K  Q  G+  +      +F+  PG G+   V+ K + LVGNR+LM  N I +   V+
Sbjct: 727 TNNKDLQNSGWLLD----TSDFSALPGKGIQCLVNEKMI-LVGNRKLMSENAINIPDHVE 786

Query: 699 RYIADNEHKAQTCILVAINGRVAGGFGVNDPPKPGAKAVISYLRSKGISSIMVTGDNRFT 758
           +++ D E   +T ++VA NG++ G  G+ DP K  A  V+  L   G+  IMVTGDN  T
Sbjct: 787 KFVEDLEESGKTGVIVAYNGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMVTGDNWRT 846

Query: 759 AAALARDLGIDDVLSEVDPIGKANKIKALKMTGDIVAMVGDGVNDSPALAASDIGIAIGA 818
           A A+A+++GI+DV +EV P GKA+ I++L+  G  VAMVGDG+NDSPALAA+D+G+AIGA
Sbjct: 847 ARAVAKEVGIEDVRAEVMPAGKADVIRSLQKDGSTVAMVGDGINDSPALAAADVGMAIGA 906

Query: 819 GTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWALGYNIIVVPIAAGILYPF 878
           GTD+AIEAAD VLMR+N+EDV+TAIDLSR+T  RI  NY++A+ YN++ +PIAAG+ +P 
Sbjct: 907 GTDVAIEAADYVLMRNNLEDVITAIDLSRKTLTRIRLNYVFAMAYNVVSIPIAAGVFFPV 966

Query: 879 TGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRP 893
              +LPPW AGACMA SSVSVVCSSLLL+ +K+P
Sbjct: 967 LRVQLPPWAAGACMALSSVSVVCSSLLLRRYKKP 990

BLAST of MC02g0093 vs. TAIR 10
Match: AT4G33520.2 (P-type ATP-ase 1 )

HSP 1 Score: 347.1 bits (889), Expect = 4.4e-95
Identity = 237/648 (36.57%), Postives = 356/648 (54.94%), Query Frame = 0

Query: 255 LCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGED 314
           LC       G +  +    +L + S NM+ LV LG  +++  S    + A   K    + 
Sbjct: 291 LCLITLLGPGRKLVLDGIKSLLKGSPNMNTLVGLGALSSFSVS---SLAAMIPK-LGWKT 350

Query: 315 FFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDT 374
           FFE   MLI+F+LLG+ LE  AK K +  +  L  + P  + LL   D  N  + VE+  
Sbjct: 351 FFEEPVMLIAFVLLGRNLEQRAKIKATSDMTGLLSVLPSKARLLLDGDLQN--STVEVPC 410

Query: 375 QMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGC 434
             L   D++ I+PG +VP DG+V  G+S ++ES+ TGE  P  K+ G +V +G++N NG 
Sbjct: 411 NSLSVGDLVVILPGDRVPADGVVKSGRSTIDESSFTGEPLPVTKESGSQVAAGSINLNGT 470

Query: 435 LFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLIC 494
           L V+    G +T +  I++LVE AQ   A  Q+L D+++  F   V+ ++  T+  W   
Sbjct: 471 LTVEVHRSGGETAVGDIIRLVEEAQSREAPVQQLVDKVAGRFTYGVMALSAATFTFW--- 530

Query: 495 GEAGIYPKHWIPKGM---DEFELALQFGISVLVIACPCALGLATPTAVMVASGKGASLGV 554
               ++  H +P  +       LALQ   SVLV+ACPCALGLATPTA++V +  GA  G+
Sbjct: 531 ---NLFGAHVLPSALHNGSPMSLALQLSCSVLVVACPCALGLATPTAMLVGTSLGARRGL 590

Query: 555 LIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSN--------FSMAELCHVAMA 614
           L++G   LE    V TVVFDKTGTLT G P V  V++  N        +S  E+  +A A
Sbjct: 591 LLRGGDILEKFSLVDTVVFDKTGTLTKGHPVVTEVIIPENPRHNLNDTWSEVEVLMLAAA 650

Query: 615 IESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALVGNR 674
           +ESN+ HP+  A+V+ A + +      AE     E     PG+G    V++K +  VG  
Sbjct: 651 VESNTTHPVGKAIVK-AARARNCQTMKAEDGTFTEE----PGSGAVAIVNNKRVT-VGTL 710

Query: 675 RLMRANNIRMGTEVDRYIADNEHKA--QTCILVAINGRVAGGFGVNDPPKPGAKAVISYL 734
             ++    R G   +  +A  EH+   Q+ + + ++  +A      D  +  A  V+  L
Sbjct: 711 EWVK----RHGATGNSLLALEEHEINNQSVVYIGVDNTLAAVIRFEDKVREDAAQVVENL 770

Query: 735 RSKGISSIMVTGDNRFTAAALARDLGI--DDVLSEVDPIGKANKIKALKMTGDIVAMVGD 794
             +GI   M++GD R  A  +A  +GI  + V++ V P  K N I  L+    IVAMVGD
Sbjct: 771 TRQGIDVYMLSGDKRNAANYVASVVGINHERVIAGVKPAEKKNFINELQKNKKIVAMVGD 830

Query: 795 GVNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIW 854
           G+ND+ ALA+S++G+A+G G   A E + +VLM + +  ++ A++LSRQT   +  N  W
Sbjct: 831 GINDAAALASSNVGVAMGGGAGAASEVSPVVLMGNRLTQLLDAMELSRQTMKTVKQNLWW 890

Query: 855 ALGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLK 888
           A GYNI+ +PIAAG+L P TGT L P +AGA M  SS+ V+ +SLLL+
Sbjct: 891 AFGYNIVGIPIAAGVLLPLTGTMLTPSMAGALMGVSSLGVMTNSLLLR 916

BLAST of MC02g0093 vs. TAIR 10
Match: AT4G33520.3 (P-type ATP-ase 1 )

HSP 1 Score: 347.1 bits (889), Expect = 4.4e-95
Identity = 237/648 (36.57%), Postives = 356/648 (54.94%), Query Frame = 0

Query: 255 LCTPVQFIVGWRFYVGSYYALRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGED 314
           LC       G +  +    +L + S NM+ LV LG  +++  S    + A   K    + 
Sbjct: 291 LCLITLLGPGRKLVLDGIKSLLKGSPNMNTLVGLGALSSFSVS---SLAAMIPK-LGWKT 350

Query: 315 FFETSAMLISFILLGKYLEVIAKGKTSDALAKLARLAPDTSCLLTLDDNNNILAEVEIDT 374
           FFE   MLI+F+LLG+ LE  AK K +  +  L  + P  + LL   D  N  + VE+  
Sbjct: 351 FFEEPVMLIAFVLLGRNLEQRAKIKATSDMTGLLSVLPSKARLLLDGDLQN--STVEVPC 410

Query: 375 QMLQRNDVIKIVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGC 434
             L   D++ I+PG +VP DG+V  G+S ++ES+ TGE  P  K+ G +V +G++N NG 
Sbjct: 411 NSLSVGDLVVILPGDRVPADGVVKSGRSTIDESSFTGEPLPVTKESGSQVAAGSINLNGT 470

Query: 435 LFVKATHVGADTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLIC 494
           L V+    G +T +  I++LVE AQ   A  Q+L D+++  F   V+ ++  T+  W   
Sbjct: 471 LTVEVHRSGGETAVGDIIRLVEEAQSREAPVQQLVDKVAGRFTYGVMALSAATFTFW--- 530

Query: 495 GEAGIYPKHWIPKGM---DEFELALQFGISVLVIACPCALGLATPTAVMVASGKGASLGV 554
               ++  H +P  +       LALQ   SVLV+ACPCALGLATPTA++V +  GA  G+
Sbjct: 531 ---NLFGAHVLPSALHNGSPMSLALQLSCSVLVVACPCALGLATPTAMLVGTSLGARRGL 590

Query: 555 LIKGASALENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSN--------FSMAELCHVAMA 614
           L++G   LE    V TVVFDKTGTLT G P V  V++  N        +S  E+  +A A
Sbjct: 591 LLRGGDILEKFSLVDTVVFDKTGTLTKGHPVVTEVIIPENPRHNLNDTWSEVEVLMLAAA 650

Query: 615 IESNSKHPLANAVVQHAKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLALVGNR 674
           +ESN+ HP+  A+V+ A + +      AE     E     PG+G    V++K +  VG  
Sbjct: 651 VESNTTHPVGKAIVK-AARARNCQTMKAEDGTFTEE----PGSGAVAIVNNKRVT-VGTL 710

Query: 675 RLMRANNIRMGTEVDRYIADNEHKA--QTCILVAINGRVAGGFGVNDPPKPGAKAVISYL 734
             ++    R G   +  +A  EH+   Q+ + + ++  +A      D  +  A  V+  L
Sbjct: 711 EWVK----RHGATGNSLLALEEHEINNQSVVYIGVDNTLAAVIRFEDKVREDAAQVVENL 770

Query: 735 RSKGISSIMVTGDNRFTAAALARDLGI--DDVLSEVDPIGKANKIKALKMTGDIVAMVGD 794
             +GI   M++GD R  A  +A  +GI  + V++ V P  K N I  L+    IVAMVGD
Sbjct: 771 TRQGIDVYMLSGDKRNAANYVASVVGINHERVIAGVKPAEKKNFINELQKNKKIVAMVGD 830

Query: 795 GVNDSPALAASDIGIAIGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIW 854
           G+ND+ ALA+S++G+A+G G   A E + +VLM + +  ++ A++LSRQT   +  N  W
Sbjct: 831 GINDAAALASSNVGVAMGGGAGAASEVSPVVLMGNRLTQLLDAMELSRQTMKTVKQNLWW 890

Query: 855 ALGYNIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLK 888
           A GYNI+ +PIAAG+L P TGT L P +AGA M  SS+ V+ +SLLL+
Sbjct: 891 AFGYNIVRIPIAAGVLLPLTGTMLTPSMAGALMGVSSLGVMTNSLLLR 916

BLAST of MC02g0093 vs. TAIR 10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 330.1 bits (845), Expect = 5.5e-90
Identity = 226/655 (34.50%), Postives = 372/655 (56.79%), Query Frame = 0

Query: 274 ALRQKSANMDVLVALGTNAAYFYSVYIVIKAFASKYFQGEDFFETSAMLISFILLGKYLE 333
           A  ++S NM+ LV LG+ AA+  S+  ++     +      FF+   ML+ F+LLG+ LE
Sbjct: 242 AFGKRSPNMNSLVGLGSMAAFSISLISLVN---PELEWDASFFDEPVMLLGFVLLGRSLE 301

Query: 334 VIAKGKTSDALAKLARLAPDTSCL-LTLDDNN----NILAE----VEIDTQMLQRNDVIK 393
             AK + S  + +L  L    S L +T  DNN    ++L+     + +    ++  D + 
Sbjct: 302 ERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICINVSVDDIRVGDSLL 361

Query: 394 IVPGAKVPVDGIVIHGQSHVNESTITGESRPTIKKPGDKVVSGTVNENGCLFVKATHVGA 453
           ++PG   PVDG V+ G+S V+ES +TGES P  K+ G  V +GT+N +G L +KA+  G+
Sbjct: 362 VLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINWDGPLRIKASSTGS 421

Query: 454 DTTLSQIVQLVESAQLSRASAQKLADQISKFFVPAVVVVAFLTWLGWLICGEAGIYPKHW 513
           ++T+S+IV++VE AQ + A  Q+LAD I+  FV  ++ ++ +T+  W   G + I+P   
Sbjct: 422 NSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFWYYVG-SHIFPDVL 481

Query: 514 I-----PKGMDEFELALQFGISVLVIACPCALGLATPTAVMVASGKGASLGVLIKGASAL 573
           +     P G D   L+L+  + VLV++CPCALGLATPTA+++ +  GA  G LI+G   L
Sbjct: 482 LNDIAGPDG-DALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLGAKRGYLIRGGDVL 541

Query: 574 ENAYKVKTVVFDKTGTLTLGRPKVVSVVLFSNFSMAELCHVAMAIESNSKHPLANAVVQH 633
           E    +  V  DKTGTLT GRP VVS V    +   E+  +A A+E  + HP+A A+V  
Sbjct: 542 ERLASIDCVALDKTGTLTEGRP-VVSGVASLGYEEQEVLKMAAAVEKTATHPIAKAIVNE 601

Query: 634 AKKVQQKYGFSAERPGHVENFAVFPGTGVGGKVDSKFLAL-----VGNRRLMR---ANNI 693
           A+ +  K   + E  G +      PG G   ++D +F+A+     V +R L +   ++ +
Sbjct: 602 AESLNLK---TPETRGQLTE----PGFGTLAEIDGRFVAVGSLEWVSDRFLKKNDSSDMV 661

Query: 694 RMGTEVDRYIADNEHKAQTCILVAINGR----VAGGFGVNDPPKPGAKAVISYLRSKGIS 753
           ++ + +D  +++    ++    V   GR    + G   ++D  +  A+  ++ L+ KGI 
Sbjct: 662 KLESLLDHKLSNTSSTSRYSKTVVYVGREGEGIIGAIAISDCLRQDAEFTVARLQEKGIK 721

Query: 754 SIMVTGDNRFTAAALARDLGI--DDVLSEVDPIGKANKIKALKMTGDIVAMVGDGVNDSP 813
           +++++GD     A +A+++GI  +     + P  K   I  L+ +G  VAMVGDG+ND+P
Sbjct: 722 TVLLSGDREGAVATVAKNVGIKSESTNYSLSPEKKFEFISNLQSSGHRVAMVGDGINDAP 781

Query: 814 ALAASDIGIA--IGAGTDIAIEAADIVLMRSNIEDVVTAIDLSRQTTYRIWFNYIWALGY 873
           +LA +D+GIA  I A  + A  AA ++L+R+ +  VV A+ L++ T  +++ N  WA+ Y
Sbjct: 782 SLAQADVGIALKIEAQENAASNAASVILVRNKLSHVVDALSLAQATMSKVYQNLAWAIAY 841

Query: 874 NIIVVPIAAGILYPFTGTRLPPWLAGACMAASSVSVVCSSLLLKCFKRPLNFQSI 899
           N+I +PIAAG+L P     + P L+G  MA SS+ VV +SLLL+  K   +  S+
Sbjct: 842 NVISIPIAAGVLLPQYDFAMTPSLSGGLMALSSIFVVSNSLLLQLHKSETSKNSL 883