Homology
BLAST of MC02g0081 vs. ExPASy Swiss-Prot
Match:
O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)
HSP 1 Score: 967.2 bits (2499), Expect = 1.1e-280
Identity = 466/767 (60.76%), Postives = 575/767 (74.97%), Query Frame = 0
Query: 5 YEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKVQ 64
YEA F + DFGE+GA+++EN + KEMY+K + + G G ITCNSWV P +
Sbjct: 137 YEADFVIPQDFGEVGAILIENEHHKEMYVKNIVIDGFVHGKVEITCNSWVHSKFDNPD-K 196
Query: 65 RVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLG---SCSG 124
R+FFT+KSYLP TP+G+ +R ELV LRG+G GER ++RIYDYDVYNDLG S +
Sbjct: 197 RIFFTNKSYLPSQTPSGVSRLREEELVTLRGDGIGERKVFERIYDYDVYNDLGEADSNND 256
Query: 125 DLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKDS 184
D KRPVLGG E PYPRRC+TGRP + P E R + +Y+PRDEAFS +K AF ++
Sbjct: 257 DAKRPVLGGKELPYPRRCKTGRPRSKKDPLSETR--STFVYVPRDEAFSEVKSVAFSGNT 316
Query: 185 VLSFIHTIVPNL-WVHFDDKAGFSNFKDIDALYAVDGFKLPTL-ESSTSLKDDLPWIFK- 244
V S +H +VP L V D GF +F ID+L+ V G LP L + + L + +P + K
Sbjct: 317 VYSVLHAVVPALESVVTDPNLGFPHFPAIDSLFNV-GVDLPGLGDKKSGLFNVVPRLIKA 376
Query: 245 LDSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIY 304
+ T K L F+ P+ + RDKF W D +FARQTLAGLNP SI+LVTEWPL SKLDP++Y
Sbjct: 377 ISDTRKDVLLFESPQLVQRDKFSWFRDVEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVY 436
Query: 305 GPPESAFNIEMLNQKIGS-ITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRT 364
GPPES E++ ++IG+ +TV++A+Q+KKL+ILDYHD+LLPYV KV ++G+ LYGSRT
Sbjct: 437 GPPESEITKELIEKEIGNYMTVEQAVQQKKLFILDYHDLLLPYVNKVNELKGSMLYGSRT 496
Query: 365 LFFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSE-QATHLWIWRFAKAHVLAHDAGYH 424
+FFL TLKPLAIELTRPP+DDKPQWK VY P++ AT W+W+ AKAHVL+HD+GYH
Sbjct: 497 IFFLTPQGTLKPLAIELTRPPVDDKPQWKEVYSPNDWNATGAWLWKLAKAHVLSHDSGYH 556
Query: 425 QLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIE 484
QLVSHWLRTHC EPY IA+NRQLSAMHPIYRLLHPHFRYT+EINA AR LI+A G IE
Sbjct: 557 QLVSHWLRTHCCTEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALAREALINANGVIE 616
Query: 485 FTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFAND 544
+F P KY++ELSS AY +W+FD +ALP +LI RGLA EDP HGLKL I+DYPFAND
Sbjct: 617 SSFFPGKYAIELSSIAYGAEWRFDQEALPQNLISRGLAVEDPNEPHGLKLAIEDYPFAND 676
Query: 545 GLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQ 604
GL+LWD LK+WVT YVN+YY ++++ SD ELQ WW+EI+ VGH DK+D WP L TP
Sbjct: 677 GLVLWDILKQWVTNYVNHYYPQTNLIESDKELQAWWSEIKNVGHGDKRDEPWWPELKTPN 736
Query: 605 DLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPE 664
DLI I+T I WV SGHHAAVNFGQY+YAGYFPNRP++ ++ MPTEDP W+ F+ KPE
Sbjct: 737 DLIGIITTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVARSKMPTEDPTAEEWEWFMNKPE 796
Query: 665 HFLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKN 724
LL FPSQ QATKVM +L++LS+HSPDEEY+G+ +EP W +DPVI AAF+ FS K+K
Sbjct: 797 EALLRCFPSQIQATKVMAILDVLSNHSPDEEYIGEKIEPYWAEDPVINAAFEVFSGKLKE 856
Query: 725 LEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
LE IID RN D L NR GAGV PYELLKP+SE GVTGKGVPYS+SI
Sbjct: 857 LEGIIDARNNDSKLSNRNGAGVMPYELLKPYSEPGVTGKGVPYSISI 899
BLAST of MC02g0081 vs. ExPASy Swiss-Prot
Match:
R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)
HSP 1 Score: 911.4 bits (2354), Expect = 7.0e-264
Identity = 448/765 (58.56%), Postives = 553/765 (72.29%), Query Frame = 0
Query: 5 YEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKVQ 64
Y+ FEV DFGEIGAV+V+N ++ Y+K + L + T TC+SW+ P +
Sbjct: 144 YQCEFEVPDDFGEIGAVLVQNERHRDAYVKNIVLDEIV----TFTCDSWIHSKFDNPD-K 203
Query: 65 RVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLG--SCSGD 124
R+FF +KSYLP TP GLKS+R +L +LRGNG GER +DRIYDYD YND+G D
Sbjct: 204 RIFFLNKSYLPSETPEGLKSLRQKDLESLRGNGEGERQSFDRIYDYDTYNDIGDPDTDSD 263
Query: 125 LKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKDSV 184
+ RPVLGG EHP+PRRCRTGR P E R Y+PRDE F+ IKQ ++
Sbjct: 264 MARPVLGGNEHPFPRRCRTGRKMTSTEP-WSESRTTLPFYVPRDEDFAEIKQITRGATTL 323
Query: 185 LSFIHTIVPNL-WVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFK--L 244
S +H ++P L V D+ GF F+DI+ LY P +S T LP + K
Sbjct: 324 YSVLHGVIPALSSVLKDEDKGFPLFRDIELLYEKGVDIDPPPDSGT--LSALPRLVKAIT 383
Query: 245 DSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 304
+ST K+ L+F+ P +D F W DE+F RQTLAGLNP SIQLVTEWPL+SKLDPE+YG
Sbjct: 384 NSTKKV-LQFETPRIKHKDSFSWFRDEEFCRQTLAGLNPYSIQLVTEWPLMSKLDPEVYG 443
Query: 305 PPESAFNIEMLNQKI-GSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTL 364
P ESA E + ++I G +T +EA+++K+L++LDYHD+LLPYV KVR +EGTTLYGSRTL
Sbjct: 444 PAESAITKETVEEEIKGFMTXEEALEQKRLFLLDYHDLLLPYVNKVREIEGTTLYGSRTL 503
Query: 365 FFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQL 424
FL TL+PLAIELTRPP + KPQWK VY P AT W+W+ AKAHVLAHD+GYHQL
Sbjct: 504 MFLTCTGTLRPLAIELTRPPNNGKPQWKHVYTPCWDATDXWLWKLAKAHVLAHDSGYHQL 563
Query: 425 VSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFT 484
VSHWLRTHC EPY IATNRQLS MHPI RLL PH RYT++IN AR +LI+A G IE +
Sbjct: 564 VSHWLRTHCVTEPYIIATNRQLSKMHPIQRLLCPHLRYTMQINGLARLSLINANGIIESS 623
Query: 485 FSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGL 544
FSP+KYSM+LSS AY +W+FD +ALPADLI RG+A ED +A HG+KL I+DYPFANDGL
Sbjct: 624 FSPRKYSMQLSSDAYAQKWRFDHEALPADLISRGMAVEDESAPHGIKLTIEDYPFANDGL 683
Query: 545 ILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDL 604
+LWDA+K+W T Y+N+YY + +V SD ELQ WWTEIRTVGHADKKD WP L T QDL
Sbjct: 684 LLWDAIKQWATAYINHYYPQAKLVESDEELQAWWTEIRTVGHADKKDEPWWPQLKTQQDL 743
Query: 605 IDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHF 664
I +V+ I WV+SGHH+AVNFGQY + GYFPNRP+I +T MP EDP W+ F+EKPE
Sbjct: 744 IGVVSTIMWVSSGHHSAVNFGQYDFGGYFPNRPTIARTKMPNEDPTFEEWEAFMEKPEDV 803
Query: 665 LLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLE 724
LL+ FP+Q QATKVM +L++LSSHSPDEEY+G +ME SW +P IK+AF+ F ++K L+
Sbjct: 804 LLNCFPTQIQATKVMAILDVLSSHSPDEEYIGTSMEASWEAEPAIKSAFEEFCGRLKKLD 863
Query: 725 VIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
IID RN D L+NRTGAG+ Y+LLKPFS GVTGKGVPYS+SI
Sbjct: 864 DIIDSRNRDPILRNRTGAGLVQYQLLKPFSGHGVTGKGVPYSISI 899
BLAST of MC02g0081 vs. ExPASy Swiss-Prot
Match:
P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)
HSP 1 Score: 890.6 bits (2300), Expect = 1.3e-257
Identity = 436/770 (56.62%), Postives = 541/770 (70.26%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
+E YE FE+ DFG +GA+ ++N +++++K V+L L G T TC SWV P +V P
Sbjct: 134 DEKYECEFEMPEDFGPVGAIKIQNQYHRQLFLKGVELK-LPGGSITFTCESWVAPKSVDP 193
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNG---TGERVPYDRIYDYDVYNDLGS 121
+R+FF+ KSYLP TP LK R EL L+G GE ++RIYDYDVYND+G
Sbjct: 194 -TKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGD 253
Query: 122 CSGD--LKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDA 181
D L RPV+GG HPYPRRC+TGR CE P E+R E Y+PRDE FS K +
Sbjct: 254 PDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGE-FYVPRDEEFSTAKGTS 313
Query: 182 FMKDSVLSFIHTIVPNL-WVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPW 241
F +VL+ + +I P + V + F +FK I L+ +G +LP L LP
Sbjct: 314 FTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFE-EGIQLP---KDAGLLPLLPR 373
Query: 242 IFK-LDSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLD 301
I K L L+FD P ++RD+F WL D++FARQTLAGLNP SIQLV EWPL+SKLD
Sbjct: 374 IIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLD 433
Query: 302 PEIYGPPESAFNIEMLNQKI-GSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLY 361
P +YG P S E++ +++ G++TV EA++ K+L++LDYHD+LLPYV KVR + TTLY
Sbjct: 434 PAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLY 493
Query: 362 GSRTLFFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDA 421
SRTLFFL++D TL+P+AIELT PP +KPQWK+V+ P AT W+W AK H ++HDA
Sbjct: 494 ASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDA 553
Query: 422 GYHQLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGG 481
GYHQL+SHWLRTH EPY IA NRQLSAMHPIYRLLHPHFRYT+EINA AR +L++ GG
Sbjct: 554 GYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGG 613
Query: 482 TIEFTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPF 541
IE F P KY++ELSS Y W+FD + LPADLI RGLAEED TA HG++L I DYPF
Sbjct: 614 IIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPF 673
Query: 542 ANDGLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLN 601
ANDGLILWDA+KEWVT+YV +YY D ++TSD ELQ WW+E+R +GH DKKD WP L
Sbjct: 674 ANDGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLK 733
Query: 602 TPQDLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLE 661
T DLI +VT IAWV SGHHAAVNFGQY Y GYFPNRP+ T+ MPTEDP K F E
Sbjct: 734 TQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYE 793
Query: 662 KPEHFLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAK 721
PE LL +PSQ QAT VM+ L++LS+HSPDEEY+G+ E SW ++PVI AAF+RF K
Sbjct: 794 SPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGK 853
Query: 722 MKNLEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
++ LE +ID RN + +LKNR GAGV YELLKP SE GVTG GVPYS+SI
Sbjct: 854 LQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896
BLAST of MC02g0081 vs. ExPASy Swiss-Prot
Match:
P38419 (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX1 PE=2 SV=2)
HSP 1 Score: 873.2 bits (2255), Expect = 2.1e-252
Identity = 433/777 (55.73%), Postives = 562/777 (72.33%), Query Frame = 0
Query: 5 YEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGL-ASGPST---ITCNSWVQPPTVL 64
YEA F+V FG IGA+IV N +EM++++++LT +G ST I CNSWVQP +V
Sbjct: 151 YEADFDVPSGFGPIGAIIVTNELRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQPKSVG 210
Query: 65 PK---VQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLG 124
+ +R+FF +K+YLP TPAGL+S R N+L RG+GTGER DR+YDYDVYNDLG
Sbjct: 211 DEGTPSKRIFFANKTYLPGQTPAGLRSYRKNDLQQKRGDGTGEREADDRVYDYDVYNDLG 270
Query: 125 S--CSGDLKRPVLGG-AEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQ 184
+ +GDL RPVLGG + PYPRRCRTGRP + P+ E R+ N +Y+PRDE FS K+
Sbjct: 271 NPDSNGDLARPVLGGNKQFPYPRRCRTGRPPSKKDPKSETRKGN--VYVPRDEEFSPEKE 330
Query: 185 DAFMKDSVLSFIHTIVPNLWVHFDDK----AGFSNFKDIDALYAVDGFKLPTLESSTSLK 244
D F++ +V S + VP DK F +F ID L+ DG +LP ++ L+
Sbjct: 331 DYFLRKTVGSVLQAAVPAAQSLLLDKLKWNLPFPSFFVIDKLFE-DGVELPGVDKLNFLE 390
Query: 245 DDLPWIFK--LDSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWP 304
+P + + D+ + LRF+ P + +DKF WL DE+FAR+TLAG+NP +I+LV E+P
Sbjct: 391 SVVPRLLEHLRDTPAEKILRFETPANIQKDKFAWLRDEEFARETLAGINPYAIELVREFP 450
Query: 305 LVSKLDPEIYGPPESAFNIEMLNQKIGSI-TVQEAIQKKKLYILDYHDMLLPYVQKVRAV 364
L SKLDP +YGP ESA ++L +++ + TV+EAI +K+L++LD+HD+ LPYV K+R++
Sbjct: 451 LKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSL 510
Query: 365 EGTTLYGSRTLFFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAH 424
+ TT+YGSRT+FFL +D TL+ LAIELTRP +PQW++V+ PS AT W+WR AKAH
Sbjct: 511 DHTTMYGSRTVFFLTDDGTLQLLAIELTRPASPSQPQWRQVFTPSTDATMSWLWRMAKAH 570
Query: 425 VLAHDAGYHQLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTN 484
V AHDAG+H+L++HWLRTHCAVEPY IA NRQLS MHPIY+LL PHFRYT+ INA AR+
Sbjct: 571 VRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLRPHFRYTMRINARARSA 630
Query: 485 LISAGGTIEFTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLN 544
LISAGG IE +FSPQKYSMELSS AYD W+FD +ALPADL+ RG+AEEDPTA HGLKL
Sbjct: 631 LISAGGIIERSFSPQKYSMELSSVAYDKLWRFDTEALPADLVRRGMAEEDPTAEHGLKLA 690
Query: 545 IKDYPFANDGLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAA 604
I+DYPFANDGL++WDA+K WV YV +Y D+ V D ELQ +WTE+RT GH DKKDA
Sbjct: 691 IEDYPFANDGLLIWDAIKTWVQAYVARFYPDADSVAGDEELQAFWTEVRTKGHGDKKDAP 750
Query: 605 GWPTLNTPQDLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDP-NPA 664
WP L++P+ L +T I WVA+ HHAAVNFGQY + GYFPNRPSI +T MP E+P + A
Sbjct: 751 WWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGA 810
Query: 665 LWKCFLEKPEHFLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAA 724
+ FL+ P+ L + FPSQ QAT VM VL++LSSHS DEEY+G W D ++AA
Sbjct: 811 AMERFLDNPDQALRECFPSQVQATVVMAVLDVLSSHSTDEEYLGGEQTRPWNSDAAVQAA 870
Query: 725 FDRFSAKMKNLEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
+D F+A++K +E +ID RN D LKNR GAG+ PY+L+KPFS+SGVTG G+P S SI
Sbjct: 871 YDGFAARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSDSGVTGMGIPNSTSI 924
BLAST of MC02g0081 vs. ExPASy Swiss-Prot
Match:
Q84YK8 (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)
HSP 1 Score: 865.5 bits (2235), Expect = 4.4e-250
Identity = 428/777 (55.08%), Postives = 558/777 (71.81%), Query Frame = 0
Query: 5 YEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLT-GLASGPST---ITCNSWVQPPTVL 64
YEA F+V FG IGAV+V N +EM++++++LT G +G ST I CNSWVQP + +
Sbjct: 169 YEADFDVPTGFGPIGAVVVTNELGQEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSSI 228
Query: 65 PK---VQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLG 124
+ +R+FF +K+YLP TPAGL+S R +L RGNG G+R DR+YDYDVYNDLG
Sbjct: 229 DEGTPGKRIFF-AKAYLPGQTPAGLRSYREEDLKQKRGNGAGQREADDRVYDYDVYNDLG 288
Query: 125 S--CSGDLKRPVLGGA-EHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQ 184
+ +GDL RPVLGG+ + PYPRRCRTGRP + P+ E R+ N +Y+PRDE FS +K
Sbjct: 289 NPDSNGDLARPVLGGSKQFPYPRRCRTGRPPSKKDPKSETRKGN--VYVPRDEEFSEVKN 348
Query: 185 DAFMKDSVLSFIHTIVPNLWVHFDDKAG----FSNFKDIDALYAVDGFKLPTLESSTSLK 244
F+ ++ S +H VP D F +F ID L+ DG +LP +E L
Sbjct: 349 AQFLLKTLQSVLHAAVPAAQSALIDNLSLNLPFPSFFVIDKLFE-DGVELPGVEKLGFLH 408
Query: 245 DDLPWIFKL--DSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWP 304
+P + +L DS G L FD P + +DKF WL DE+FAR+TLAG+NP +I+LV E+P
Sbjct: 409 SIVPRLLELLRDSPGDKILLFDTPANVQKDKFAWLRDEEFARETLAGINPYAIELVREFP 468
Query: 305 LVSKLDPEIYGPPESAFNIEMLNQKIGSI-TVQEAIQKKKLYILDYHDMLLPYVQKVRAV 364
L SKLDP +YGP ESA ++L +++ + TV+EAI +K+L++LD+HD+ LPYV K+R++
Sbjct: 469 LKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSL 528
Query: 365 EGTTLYGSRTLFFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAH 424
+ TT+YGSRT+FFL +D TL+ LAIELTRP +PQW++V+ PS T W+WR AKAH
Sbjct: 529 KHTTMYGSRTIFFLTDDGTLRLLAIELTRPASPSQPQWRQVFTPSTDTTKSWLWRMAKAH 588
Query: 425 VLAHDAGYHQLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTN 484
V AHDAG+H+L++HWLRTHCAVEPY IA NRQLS MHPIY+LLHPHFRYT+ INA AR+
Sbjct: 589 VRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLHPHFRYTMRINALARSR 648
Query: 485 LISAGGTIEFTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLN 544
LISA G IE +FSPQKYSMELSS AYD W+FD++ALPADL+ RG+AEEDPTA HGL+L
Sbjct: 649 LISAAGIIELSFSPQKYSMELSSVAYDKLWRFDMEALPADLVRRGMAEEDPTAEHGLRLA 708
Query: 545 IKDYPFANDGLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAA 604
I+DYPFANDGL++WDA+K WV YV +Y D+ V D ELQ +WTE+RT GH DKKDA
Sbjct: 709 IEDYPFANDGLLIWDAIKTWVQAYVARFYPDADSVAGDEELQAFWTEVRTKGHGDKKDAP 768
Query: 605 GWPTLNTPQDLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDP-NPA 664
WP L++P+ L +T I WVA+ HHAAVNFGQY + GYFPNRPSI +T MP E+P + A
Sbjct: 769 WWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGA 828
Query: 665 LWKCFLEKPEHFLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAA 724
+ FL+ P+ L + FPSQ QAT VM VL++LS+HS DEEY+G W D ++AA
Sbjct: 829 AMERFLDNPDQALRECFPSQVQATVVMAVLDVLSTHSTDEEYLGGEQTRPWNSDAAVQAA 888
Query: 725 FDRFSAKMKNLEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
+ F+A++K +E +ID RN D LKNR GAG+ PY+L+KPFS++GVTG G+P S SI
Sbjct: 889 YAGFTARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSDAGVTGMGIPNSTSI 941
BLAST of MC02g0081 vs. NCBI nr
Match:
XP_022148710.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])
HSP 1 Score: 1582 bits (4095), Expect = 0.0
Identity = 763/763 (100.00%), Postives = 763/763 (100.00%), Query Frame = 0
Query: 1 KEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVL 60
KEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVL
Sbjct: 142 KEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVL 201
Query: 61 PKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS 120
PKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS
Sbjct: 202 PKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS 261
Query: 121 GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD 180
GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD
Sbjct: 262 GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD 321
Query: 181 SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD 240
SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD
Sbjct: 322 SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD 381
Query: 241 STGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYGP 300
STGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYGP
Sbjct: 382 STGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYGP 441
Query: 301 PESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLFF 360
PESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLFF
Sbjct: 442 PESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLFF 501
Query: 361 LNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLVS 420
LNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLVS
Sbjct: 502 LNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLVS 561
Query: 421 HWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTFS 480
HWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTFS
Sbjct: 562 HWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTFS 621
Query: 481 PQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLIL 540
PQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLIL
Sbjct: 622 PQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLIL 681
Query: 541 WDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLID 600
WDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLID
Sbjct: 682 WDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLID 741
Query: 601 IVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFLL 660
IVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFLL
Sbjct: 742 IVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFLL 801
Query: 661 DAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEVI 720
DAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEVI
Sbjct: 802 DAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEVI 861
Query: 721 IDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
IDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI
Sbjct: 862 IDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 904
BLAST of MC02g0081 vs. NCBI nr
Match:
XP_022153765.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])
HSP 1 Score: 1372 bits (3552), Expect = 0.0
Identity = 664/764 (86.91%), Postives = 701/764 (91.75%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP
Sbjct: 142 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 201
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSC-S 121
KVQRVFFTSKSYLP TPAGLKSMRA ELVNLRGNGTGER DRIYDYDVYNDLG C S
Sbjct: 202 KVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERKATDRIYDYDVYNDLGDCTS 261
Query: 122 GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD 181
+LKRPVLGG E PYPRRCRTGRPHC P+ EER +IY+PRDE FS IKQ AF D
Sbjct: 262 EELKRPVLGGPERPYPRRCRTGRPHCTEDPDSEERTTG-AIYVPRDETFSTIKQGAFNVD 321
Query: 182 SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD 241
++LS + TI+P L VHFD GF NFK IDALY VDGF LP LES+ SLKD LPWIFKL
Sbjct: 322 TILSVLRTILPRLQVHFDRDTGFKNFKSIDALYDVDGFNLPPLESTDSLKDLLPWIFKLV 381
Query: 242 S-TGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 301
S TG+ LRF PEPMDRDKFFWLSDE+FARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG
Sbjct: 382 SETGEFFLRFQSPEPMDRDKFFWLSDEEFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 441
Query: 302 PPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLF 361
PPESAFN EMLNQ+IGS+TVQEAIQKKKLYILDYHDMLLPYVQKVRA++GTTLYGSRTLF
Sbjct: 442 PPESAFNTEMLNQQIGSMTVQEAIQKKKLYILDYHDMLLPYVQKVRALKGTTLYGSRTLF 501
Query: 362 FLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV 421
FLNEDETL+PLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV
Sbjct: 502 FLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV 561
Query: 422 SHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTF 481
SHW+RTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINA+AR+NLI+AGGTIE TF
Sbjct: 562 SHWVRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINAAARSNLINAGGTIETTF 621
Query: 482 SPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLI 541
SP KYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGL+LNIKDYPFANDGLI
Sbjct: 622 SPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLRLNIKDYPFANDGLI 681
Query: 542 LWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLI 601
LWDALK+WVTEYVN+YY D ++TSD ELQ WWTEIRTVGHADKKDAAGWPTLNTPQDLI
Sbjct: 682 LWDALKQWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKDAAGWPTLNTPQDLI 741
Query: 602 DIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFL 661
+IV+NIAWVAS HHAAVNFGQYAYAGYFPNRPSI +TNMPTEDPNPALWK FLEKPE L
Sbjct: 742 EIVSNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDPNPALWKSFLEKPEDVL 801
Query: 662 LDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEV 721
LDAFPSQYQAT+VMMVL++LSSHSPDEEY+GKNMEPSWGDDPVIKAAFD+F+AKMK LE+
Sbjct: 802 LDAFPSQYQATQVMMVLDVLSSHSPDEEYIGKNMEPSWGDDPVIKAAFDKFNAKMKELEL 861
Query: 722 IIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
I+D RNAD LKNRTGAGV+PYELLKPFS GVTGKGVPYSVSI
Sbjct: 862 IVDLRNADLDLKNRTGAGVTPYELLKPFSGPGVTGKGVPYSVSI 904
BLAST of MC02g0081 vs. NCBI nr
Match:
XP_038901038.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Benincasa hispida])
HSP 1 Score: 1275 bits (3299), Expect = 0.0
Identity = 616/765 (80.52%), Postives = 677/765 (88.50%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
EEIYE S EV DFGEIGAVI+EN NE EM+IKEVDL+GL+SGP TI+CNSWVQP T++P
Sbjct: 143 EEIYETSLEVPTDFGEIGAVIIENHNENEMFIKEVDLSGLSSGPLTISCNSWVQPKTLVP 202
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGS--C 121
RVFFT+KSYLP TPAGLKSMR ELVNLRGNGTGER YDRIYDYDVYNDLG+
Sbjct: 203 TQCRVFFTNKSYLPSQTPAGLKSMRETELVNLRGNGTGERQSYDRIYDYDVYNDLGNPDM 262
Query: 122 SGDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMK 181
S DLKRP+LGG +HPYPRRCRTGRP + P+ E+R SIY+PRDE FS+IKQ F
Sbjct: 263 SEDLKRPILGGLDHPYPRRCRTGRPPTQTDPQSEQRATG-SIYVPRDETFSDIKQKVFNA 322
Query: 182 DSVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKL 241
++LS + TI+P L VHFD AGF NFK +DALY VDGF LP LES+TSLK+ LPWIFKL
Sbjct: 323 STMLSVLRTIIPRLQVHFDQNAGFLNFKAVDALYDVDGFNLPPLESTTSLKELLPWIFKL 382
Query: 242 DS-TGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIY 301
S TG+ RF PEPMDRDKFFWL DE+FARQTLAGLNPCSIQLV EWPL S+LDPE+Y
Sbjct: 383 ISETGEFLFRFQSPEPMDRDKFFWLRDEEFARQTLAGLNPCSIQLVKEWPLSSQLDPEVY 442
Query: 302 GPPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTL 361
GPPESAFN +M++Q+IGS+TVQEAIQKKKL+ILDYHDMLLPYVQKVRA++GTTLYGSRTL
Sbjct: 443 GPPESAFNTQMIDQEIGSMTVQEAIQKKKLFILDYHDMLLPYVQKVRALKGTTLYGSRTL 502
Query: 362 FFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQL 421
FFLNEDETL+PLAIELTRPPMD KPQWKRVYGPSE+AT LW+WRFAKAHVLAHDAGYHQL
Sbjct: 503 FFLNEDETLRPLAIELTRPPMDGKPQWKRVYGPSEKATSLWLWRFAKAHVLAHDAGYHQL 562
Query: 422 VSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFT 481
VSHWLRTHCAVEPY IATNRQLSAMHPIYRLLHPHFRYT+EINASARTNLI+AGGTIE T
Sbjct: 563 VSHWLRTHCAVEPYVIATNRQLSAMHPIYRLLHPHFRYTMEINASARTNLINAGGTIEST 622
Query: 482 FSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGL 541
FSP KYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPT+SHGLKL+IKDYPFANDGL
Sbjct: 623 FSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTSSHGLKLHIKDYPFANDGL 682
Query: 542 ILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDL 601
ILWDALK+WVTEYVN+YY D +V SD ELQ WWTEIRTVGHADK++ WP LNTPQDL
Sbjct: 683 ILWDALKQWVTEYVNHYYPDPYLVISDTELQAWWTEIRTVGHADKQEEPWWPVLNTPQDL 742
Query: 602 IDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHF 661
IDIVTNIAWVAS HHAAVNFGQYAYAGYFPNRPSIT+TNMPTED NPALWK FLEKPE
Sbjct: 743 IDIVTNIAWVASAHHAAVNFGQYAYAGYFPNRPSITRTNMPTEDYNPALWKSFLEKPEEL 802
Query: 662 LLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLE 721
LL+AFPSQYQAT+VM+VLN+LSSHSPDEEY+GKNMEPSW D+ VIKAAF++FSAKMK LE
Sbjct: 803 LLNAFPSQYQATQVMLVLNVLSSHSPDEEYLGKNMEPSWNDNHVIKAAFEKFSAKMKELE 862
Query: 722 VIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
+IID RN+D +LKNRTGAGV+PYELLKP S GVTGKGVPYS+SI
Sbjct: 863 LIIDHRNSDSNLKNRTGAGVTPYELLKPVSGPGVTGKGVPYSISI 906
BLAST of MC02g0081 vs. NCBI nr
Match:
XP_022153766.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])
HSP 1 Score: 1274 bits (3298), Expect = 0.0
Identity = 620/764 (81.15%), Postives = 672/764 (87.96%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
EEIYEASF+V DFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP
Sbjct: 140 EEIYEASFQVASDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 199
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS- 121
KVQRVFFTSKSYLP TPAGLKSMRA ELVNLRGNGTGER D IYDYDVYNDLG C+
Sbjct: 200 KVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERKSTDNIYDYDVYNDLGDCTL 259
Query: 122 GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD 181
+L+RPVLGG E PYPRRCRTGRPHC+ P+ EE+ S+Y+PRD+ FSN+KQ F D
Sbjct: 260 EELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVG-SMYVPRDQTFSNVKQAVFTID 319
Query: 182 SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD 241
+ P L + FD KA FSNF++IDAL++ DGF L LES TSL D LPW FKL
Sbjct: 320 ----LLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGFNLRNLESPTSLNDLLPWRFKLV 379
Query: 242 S-TGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 301
S T + L F PEPMDRDKFFWLSDE+FAR+TLAGLNP SIQLVTEWPL+SKLDPEIYG
Sbjct: 380 SETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQLVTEWPLMSKLDPEIYG 439
Query: 302 PPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLF 361
PPESAFN +ML+Q+IGS+TVQEAIQKKKL+I+DYHDMLLPYVQKVRA++GTTLYGSRTLF
Sbjct: 440 PPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVRALKGTTLYGSRTLF 499
Query: 362 FLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV 421
FLNEDETL+PLAIELTRPPMDDKPQWKRVYGPSEQATHLW+WRFAKAH L +DAGYHQL+
Sbjct: 500 FLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAKAHALVNDAGYHQLI 559
Query: 422 SHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTF 481
+HWLRTHC+VEPYCIATNRQLSAMHPIYRLLHPHFRYT+EINA AR+NLI+A G IE TF
Sbjct: 560 THWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADARSNLINADGIIETTF 619
Query: 482 SPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLI 541
S +KYSMELSSTAY LQWQFDLQALPADLIHRGLAEEDP ASHGLKLNIKDYPFANDGLI
Sbjct: 620 STKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLKLNIKDYPFANDGLI 679
Query: 542 LWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLI 601
LWDALKEWVTEYVN+YY D ++TSD ELQ WWTEIRTVGHADKK A GWP LNTPQDLI
Sbjct: 680 LWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKYAPGWPALNTPQDLI 739
Query: 602 DIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFL 661
DI+TNIAWVAS HHAAVNFGQYAYAGYFPNRPSI +TNMPTED NPALW+ FLEKPE L
Sbjct: 740 DIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNPALWRSFLEKPEDVL 799
Query: 662 LDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEV 721
LDAFPS QAT VM+VL++LSSHSPDEEY+GK MEPSWGDDPVIKAAFDRFS KMK LEV
Sbjct: 800 LDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKAAFDRFSMKMKELEV 859
Query: 722 IIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
IID RNAD +LKNRTGAG++PYELLKPFS GVTGKGVP SVSI
Sbjct: 860 IIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 898
BLAST of MC02g0081 vs. NCBI nr
Match:
XP_022958148.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])
HSP 1 Score: 1254 bits (3244), Expect = 0.0
Identity = 603/765 (78.82%), Postives = 670/765 (87.58%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
EEIYEAS EV DFG+IGAVIVEN NEKE ++KEV+L GLASGP +TCNSWVQP T++P
Sbjct: 139 EEIYEASLEVPADFGDIGAVIVENHNEKEKFVKEVELNGLASGPLIVTCNSWVQPKTLVP 198
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSC-- 121
RVFFT KSYLP TPAGL+SMR ELVNLRGNGTGER YDRIYDYDVYNDLG
Sbjct: 199 TQNRVFFTDKSYLPSQTPAGLRSMRETELVNLRGNGTGERQSYDRIYDYDVYNDLGDPDK 258
Query: 122 SGDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMK 181
S DLKRPVLGG E PYPRRCRTGRP + P E+R SIY+PRDE FS++KQ F
Sbjct: 259 SEDLKRPVLGGTERPYPRRCRTGRPPTKTDPNSEQRATG-SIYVPRDETFSDLKQGVFNA 318
Query: 182 DSVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFK- 241
++LS + I+P L VHFD GF NFK +DAL+ VDGF LP LES+TSLKD LPWIFK
Sbjct: 319 STLLSVLRAIIPRLQVHFDQNTGFPNFKAVDALFDVDGFNLPPLESTTSLKDLLPWIFKF 378
Query: 242 LDSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIY 301
L TG+ RF PEPMDRDKFFWL DE+FARQTLAGLNPCSIQLV EWPL+S+L+PEIY
Sbjct: 379 LSETGQFLFRFQSPEPMDRDKFFWLRDEEFARQTLAGLNPCSIQLVKEWPLMSQLNPEIY 438
Query: 302 GPPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTL 361
GPPESAFN +M++++IGS+TVQEAIQ+KKL+ILDY+DMLLPYV++VRA++GTTLYGSRTL
Sbjct: 439 GPPESAFNTQMIDREIGSMTVQEAIQQKKLFILDYNDMLLPYVRRVRALKGTTLYGSRTL 498
Query: 362 FFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQL 421
FFLNED TL+PLAIELTRPPMD KPQWK VYGPSE AT+LW+WRFAK+HVLAHDAGYHQL
Sbjct: 499 FFLNEDGTLRPLAIELTRPPMDGKPQWKGVYGPSENATNLWLWRFAKSHVLAHDAGYHQL 558
Query: 422 VSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFT 481
VSHWLRTHCAVEPY IATNRQLSAMHPI+RLL+PHFRYT+EINA+ARTNLI+AGGTIE T
Sbjct: 559 VSHWLRTHCAVEPYVIATNRQLSAMHPIHRLLNPHFRYTMEINAAARTNLINAGGTIEST 618
Query: 482 FSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGL 541
FSP KYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKL+IKDYPFANDGL
Sbjct: 619 FSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLHIKDYPFANDGL 678
Query: 542 ILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDL 601
ILWDALK+WVTEYVN+YY D S+V SD ELQ WWTEIRTVGHADK+D WP LNTPQDL
Sbjct: 679 ILWDALKQWVTEYVNHYYPDPSLVASDTELQSWWTEIRTVGHADKQDEPWWPLLNTPQDL 738
Query: 602 IDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHF 661
IDIVTNIAWVAS HHAAVNFGQYAYAGYFP+RPSI +TNMPTED NPALWK FLEKPE
Sbjct: 739 IDIVTNIAWVASAHHAAVNFGQYAYAGYFPSRPSIARTNMPTEDTNPALWKSFLEKPEEV 798
Query: 662 LLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLE 721
LL+AFPSQYQAT+VM+VL++LSSHSPDEEY+GKNMEPSWGD+P IKAAFDRF+ +MK LE
Sbjct: 799 LLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWGDNPAIKAAFDRFNKRMKELE 858
Query: 722 VIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
+IID RNADF+LKNRTGAGV+PYELLKPFS GVTGKGVPYS+SI
Sbjct: 859 LIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVPYSISI 902
BLAST of MC02g0081 vs. ExPASy TrEMBL
Match:
A0A6J1D3N9 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017305 PE=3 SV=1)
HSP 1 Score: 1582 bits (4095), Expect = 0.0
Identity = 763/763 (100.00%), Postives = 763/763 (100.00%), Query Frame = 0
Query: 1 KEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVL 60
KEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVL
Sbjct: 142 KEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVL 201
Query: 61 PKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS 120
PKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS
Sbjct: 202 PKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS 261
Query: 121 GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD 180
GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD
Sbjct: 262 GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD 321
Query: 181 SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD 240
SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD
Sbjct: 322 SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD 381
Query: 241 STGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYGP 300
STGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYGP
Sbjct: 382 STGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYGP 441
Query: 301 PESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLFF 360
PESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLFF
Sbjct: 442 PESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLFF 501
Query: 361 LNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLVS 420
LNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLVS
Sbjct: 502 LNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLVS 561
Query: 421 HWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTFS 480
HWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTFS
Sbjct: 562 HWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTFS 621
Query: 481 PQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLIL 540
PQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLIL
Sbjct: 622 PQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLIL 681
Query: 541 WDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLID 600
WDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLID
Sbjct: 682 WDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLID 741
Query: 601 IVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFLL 660
IVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFLL
Sbjct: 742 IVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFLL 801
Query: 661 DAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEVI 720
DAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEVI
Sbjct: 802 DAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEVI 861
Query: 721 IDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
IDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI
Sbjct: 862 IDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 904
BLAST of MC02g0081 vs. ExPASy TrEMBL
Match:
A0A6J1DHR2 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111021205 PE=3 SV=1)
HSP 1 Score: 1372 bits (3552), Expect = 0.0
Identity = 664/764 (86.91%), Postives = 701/764 (91.75%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP
Sbjct: 142 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 201
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSC-S 121
KVQRVFFTSKSYLP TPAGLKSMRA ELVNLRGNGTGER DRIYDYDVYNDLG C S
Sbjct: 202 KVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERKATDRIYDYDVYNDLGDCTS 261
Query: 122 GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD 181
+LKRPVLGG E PYPRRCRTGRPHC P+ EER +IY+PRDE FS IKQ AF D
Sbjct: 262 EELKRPVLGGPERPYPRRCRTGRPHCTEDPDSEERTTG-AIYVPRDETFSTIKQGAFNVD 321
Query: 182 SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD 241
++LS + TI+P L VHFD GF NFK IDALY VDGF LP LES+ SLKD LPWIFKL
Sbjct: 322 TILSVLRTILPRLQVHFDRDTGFKNFKSIDALYDVDGFNLPPLESTDSLKDLLPWIFKLV 381
Query: 242 S-TGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 301
S TG+ LRF PEPMDRDKFFWLSDE+FARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG
Sbjct: 382 SETGEFFLRFQSPEPMDRDKFFWLSDEEFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 441
Query: 302 PPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLF 361
PPESAFN EMLNQ+IGS+TVQEAIQKKKLYILDYHDMLLPYVQKVRA++GTTLYGSRTLF
Sbjct: 442 PPESAFNTEMLNQQIGSMTVQEAIQKKKLYILDYHDMLLPYVQKVRALKGTTLYGSRTLF 501
Query: 362 FLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV 421
FLNEDETL+PLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV
Sbjct: 502 FLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV 561
Query: 422 SHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTF 481
SHW+RTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINA+AR+NLI+AGGTIE TF
Sbjct: 562 SHWVRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINAAARSNLINAGGTIETTF 621
Query: 482 SPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLI 541
SP KYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGL+LNIKDYPFANDGLI
Sbjct: 622 SPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLRLNIKDYPFANDGLI 681
Query: 542 LWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLI 601
LWDALK+WVTEYVN+YY D ++TSD ELQ WWTEIRTVGHADKKDAAGWPTLNTPQDLI
Sbjct: 682 LWDALKQWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKDAAGWPTLNTPQDLI 741
Query: 602 DIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFL 661
+IV+NIAWVAS HHAAVNFGQYAYAGYFPNRPSI +TNMPTEDPNPALWK FLEKPE L
Sbjct: 742 EIVSNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDPNPALWKSFLEKPEDVL 801
Query: 662 LDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEV 721
LDAFPSQYQAT+VMMVL++LSSHSPDEEY+GKNMEPSWGDDPVIKAAFD+F+AKMK LE+
Sbjct: 802 LDAFPSQYQATQVMMVLDVLSSHSPDEEYIGKNMEPSWGDDPVIKAAFDKFNAKMKELEL 861
Query: 722 IIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
I+D RNAD LKNRTGAGV+PYELLKPFS GVTGKGVPYSVSI
Sbjct: 862 IVDLRNADLDLKNRTGAGVTPYELLKPFSGPGVTGKGVPYSVSI 904
BLAST of MC02g0081 vs. ExPASy TrEMBL
Match:
A0A6J1DLP5 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111021206 PE=3 SV=1)
HSP 1 Score: 1274 bits (3298), Expect = 0.0
Identity = 620/764 (81.15%), Postives = 672/764 (87.96%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
EEIYEASF+V DFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP
Sbjct: 140 EEIYEASFQVASDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 199
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS- 121
KVQRVFFTSKSYLP TPAGLKSMRA ELVNLRGNGTGER D IYDYDVYNDLG C+
Sbjct: 200 KVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERKSTDNIYDYDVYNDLGDCTL 259
Query: 122 GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMKD 181
+L+RPVLGG E PYPRRCRTGRPHC+ P+ EE+ S+Y+PRD+ FSN+KQ F D
Sbjct: 260 EELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVG-SMYVPRDQTFSNVKQAVFTID 319
Query: 182 SVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFKLD 241
+ P L + FD KA FSNF++IDAL++ DGF L LES TSL D LPW FKL
Sbjct: 320 ----LLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGFNLRNLESPTSLNDLLPWRFKLV 379
Query: 242 S-TGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 301
S T + L F PEPMDRDKFFWLSDE+FAR+TLAGLNP SIQLVTEWPL+SKLDPEIYG
Sbjct: 380 SETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQLVTEWPLMSKLDPEIYG 439
Query: 302 PPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLF 361
PPESAFN +ML+Q+IGS+TVQEAIQKKKL+I+DYHDMLLPYVQKVRA++GTTLYGSRTLF
Sbjct: 440 PPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVRALKGTTLYGSRTLF 499
Query: 362 FLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV 421
FLNEDETL+PLAIELTRPPMDDKPQWKRVYGPSEQATHLW+WRFAKAH L +DAGYHQL+
Sbjct: 500 FLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAKAHALVNDAGYHQLI 559
Query: 422 SHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTF 481
+HWLRTHC+VEPYCIATNRQLSAMHPIYRLLHPHFRYT+EINA AR+NLI+A G IE TF
Sbjct: 560 THWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADARSNLINADGIIETTF 619
Query: 482 SPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLI 541
S +KYSMELSSTAY LQWQFDLQALPADLIHRGLAEEDP ASHGLKLNIKDYPFANDGLI
Sbjct: 620 STKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLKLNIKDYPFANDGLI 679
Query: 542 LWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLI 601
LWDALKEWVTEYVN+YY D ++TSD ELQ WWTEIRTVGHADKK A GWP LNTPQDLI
Sbjct: 680 LWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKYAPGWPALNTPQDLI 739
Query: 602 DIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHFL 661
DI+TNIAWVAS HHAAVNFGQYAYAGYFPNRPSI +TNMPTED NPALW+ FLEKPE L
Sbjct: 740 DIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNPALWRSFLEKPEDVL 799
Query: 662 LDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLEV 721
LDAFPS QAT VM+VL++LSSHSPDEEY+GK MEPSWGDDPVIKAAFDRFS KMK LEV
Sbjct: 800 LDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKAAFDRFSMKMKELEV 859
Query: 722 IIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
IID RNAD +LKNRTGAG++PYELLKPFS GVTGKGVP SVSI
Sbjct: 860 IIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 898
BLAST of MC02g0081 vs. ExPASy TrEMBL
Match:
A0A6J1JXK9 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111490700 PE=3 SV=1)
HSP 1 Score: 1254 bits (3244), Expect = 0.0
Identity = 602/765 (78.69%), Postives = 670/765 (87.58%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
EEIYEAS EV DFG+IGAVIVEN NEKEM++KEV+L GLASGP +TCNSWVQP T++P
Sbjct: 139 EEIYEASLEVPADFGDIGAVIVENHNEKEMFVKEVELNGLASGPLIVTCNSWVQPKTLVP 198
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSC-- 121
RVFFT KSYLP TPAGL+SMR ELVNLRGNGTGER YDR+YDYDVYNDLG
Sbjct: 199 TQNRVFFTDKSYLPSQTPAGLRSMRETELVNLRGNGTGERRSYDRVYDYDVYNDLGDPDK 258
Query: 122 SGDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMK 181
S DLKRPVLGG E PYPRRCRTGRP + P E+R SIY+PRDE FS++KQ F
Sbjct: 259 SEDLKRPVLGGTERPYPRRCRTGRPPTKTDPNSEQRATG-SIYVPRDETFSDLKQGVFNA 318
Query: 182 DSVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFK- 241
++LS + I+P L VHFD GF NFK +DAL+ VDGF LP LES+TSLKD LPWIFK
Sbjct: 319 STLLSVLRAIIPRLQVHFDQNTGFPNFKAVDALFDVDGFNLPPLESTTSLKDLLPWIFKF 378
Query: 242 LDSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIY 301
L TG+ RF PEPMDRDKFFWL DE+FARQTLAGLNPCSIQLV EWPL+S+L+PEIY
Sbjct: 379 LSETGQFLFRFQSPEPMDRDKFFWLRDEEFARQTLAGLNPCSIQLVKEWPLMSQLNPEIY 438
Query: 302 GPPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTL 361
GPPESAFN +M++++IGS+TVQEAIQ+KKL+ILDY+DMLLPYV++VRA++GTTLYGSRTL
Sbjct: 439 GPPESAFNTQMIDEEIGSMTVQEAIQEKKLFILDYNDMLLPYVRRVRALKGTTLYGSRTL 498
Query: 362 FFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQL 421
FFLNED TL+PLAIELTRPPMD KPQWK VYGPSE AT+LW+WRFAK+HVLAHDAGYHQL
Sbjct: 499 FFLNEDGTLRPLAIELTRPPMDGKPQWKGVYGPSENATNLWLWRFAKSHVLAHDAGYHQL 558
Query: 422 VSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFT 481
VSHWLRTHCAVEPY IATNRQLSAMHPI+RLL+PHFRYT+EINA+ARTNLI+AGGTIE T
Sbjct: 559 VSHWLRTHCAVEPYVIATNRQLSAMHPIHRLLNPHFRYTMEINAAARTNLINAGGTIEST 618
Query: 482 FSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGL 541
FSP KYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKL+IKDYPFANDGL
Sbjct: 619 FSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLHIKDYPFANDGL 678
Query: 542 ILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDL 601
ILWDALK+WVTEYVN+YY D S+V SD ELQ WWTEIRTVGHADK+D WP LNTPQDL
Sbjct: 679 ILWDALKQWVTEYVNHYYPDPSLVASDTELQAWWTEIRTVGHADKQDEPWWPLLNTPQDL 738
Query: 602 IDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHF 661
IDIVTNIAWVAS HHAAVNFGQYAY GYFP+RPSI +TNMPTED NPALWK FLEKPE
Sbjct: 739 IDIVTNIAWVASAHHAAVNFGQYAYGGYFPSRPSIARTNMPTEDTNPALWKSFLEKPEEV 798
Query: 662 LLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLE 721
LL+AFPSQYQAT+VM+VL++LSSHSPDEEY+GKNMEPSWGD+P IKAAFDRF+ +MK LE
Sbjct: 799 LLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWGDNPAIKAAFDRFNKRMKELE 858
Query: 722 VIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
+IID RNADF+LKNRTGAGV+PYELLKPFS GVTGKGVPYS+SI
Sbjct: 859 LIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVPYSISI 902
BLAST of MC02g0081 vs. ExPASy TrEMBL
Match:
A0A6J1H2B7 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459461 PE=3 SV=1)
HSP 1 Score: 1254 bits (3244), Expect = 0.0
Identity = 603/765 (78.82%), Postives = 670/765 (87.58%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
EEIYEAS EV DFG+IGAVIVEN NEKE ++KEV+L GLASGP +TCNSWVQP T++P
Sbjct: 139 EEIYEASLEVPADFGDIGAVIVENHNEKEKFVKEVELNGLASGPLIVTCNSWVQPKTLVP 198
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSC-- 121
RVFFT KSYLP TPAGL+SMR ELVNLRGNGTGER YDRIYDYDVYNDLG
Sbjct: 199 TQNRVFFTDKSYLPSQTPAGLRSMRETELVNLRGNGTGERQSYDRIYDYDVYNDLGDPDK 258
Query: 122 SGDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDAFMK 181
S DLKRPVLGG E PYPRRCRTGRP + P E+R SIY+PRDE FS++KQ F
Sbjct: 259 SEDLKRPVLGGTERPYPRRCRTGRPPTKTDPNSEQRATG-SIYVPRDETFSDLKQGVFNA 318
Query: 182 DSVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFK- 241
++LS + I+P L VHFD GF NFK +DAL+ VDGF LP LES+TSLKD LPWIFK
Sbjct: 319 STLLSVLRAIIPRLQVHFDQNTGFPNFKAVDALFDVDGFNLPPLESTTSLKDLLPWIFKF 378
Query: 242 LDSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIY 301
L TG+ RF PEPMDRDKFFWL DE+FARQTLAGLNPCSIQLV EWPL+S+L+PEIY
Sbjct: 379 LSETGQFLFRFQSPEPMDRDKFFWLRDEEFARQTLAGLNPCSIQLVKEWPLMSQLNPEIY 438
Query: 302 GPPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTL 361
GPPESAFN +M++++IGS+TVQEAIQ+KKL+ILDY+DMLLPYV++VRA++GTTLYGSRTL
Sbjct: 439 GPPESAFNTQMIDREIGSMTVQEAIQQKKLFILDYNDMLLPYVRRVRALKGTTLYGSRTL 498
Query: 362 FFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQL 421
FFLNED TL+PLAIELTRPPMD KPQWK VYGPSE AT+LW+WRFAK+HVLAHDAGYHQL
Sbjct: 499 FFLNEDGTLRPLAIELTRPPMDGKPQWKGVYGPSENATNLWLWRFAKSHVLAHDAGYHQL 558
Query: 422 VSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFT 481
VSHWLRTHCAVEPY IATNRQLSAMHPI+RLL+PHFRYT+EINA+ARTNLI+AGGTIE T
Sbjct: 559 VSHWLRTHCAVEPYVIATNRQLSAMHPIHRLLNPHFRYTMEINAAARTNLINAGGTIEST 618
Query: 482 FSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGL 541
FSP KYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKL+IKDYPFANDGL
Sbjct: 619 FSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLHIKDYPFANDGL 678
Query: 542 ILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDL 601
ILWDALK+WVTEYVN+YY D S+V SD ELQ WWTEIRTVGHADK+D WP LNTPQDL
Sbjct: 679 ILWDALKQWVTEYVNHYYPDPSLVASDTELQSWWTEIRTVGHADKQDEPWWPLLNTPQDL 738
Query: 602 IDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLEKPEHF 661
IDIVTNIAWVAS HHAAVNFGQYAYAGYFP+RPSI +TNMPTED NPALWK FLEKPE
Sbjct: 739 IDIVTNIAWVASAHHAAVNFGQYAYAGYFPSRPSIARTNMPTEDTNPALWKSFLEKPEEV 798
Query: 662 LLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAKMKNLE 721
LL+AFPSQYQAT+VM+VL++LSSHSPDEEY+GKNMEPSWGD+P IKAAFDRF+ +MK LE
Sbjct: 799 LLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSWGDNPAIKAAFDRFNKRMKELE 858
Query: 722 VIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 763
+IID RNADF+LKNRTGAGV+PYELLKPFS GVTGKGVPYS+SI
Sbjct: 859 LIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGVPYSISI 902
BLAST of MC02g0081 vs. TAIR 10
Match:
AT3G45140.1 (lipoxygenase 2 )
HSP 1 Score: 890.6 bits (2300), Expect = 9.0e-259
Identity = 436/770 (56.62%), Postives = 541/770 (70.26%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLP 61
+E YE FE+ DFG +GA+ ++N +++++K V+L L G T TC SWV P +V P
Sbjct: 134 DEKYECEFEMPEDFGPVGAIKIQNQYHRQLFLKGVELK-LPGGSITFTCESWVAPKSVDP 193
Query: 62 KVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNG---TGERVPYDRIYDYDVYNDLGS 121
+R+FF+ KSYLP TP LK R EL L+G GE ++RIYDYDVYND+G
Sbjct: 194 -TKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGD 253
Query: 122 CSGD--LKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRKNESIYIPRDEAFSNIKQDA 181
D L RPV+GG HPYPRRC+TGR CE P E+R E Y+PRDE FS K +
Sbjct: 254 PDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGE-FYVPRDEEFSTAKGTS 313
Query: 182 FMKDSVLSFIHTIVPNL-WVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPW 241
F +VL+ + +I P + V + F +FK I L+ +G +LP L LP
Sbjct: 314 FTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFE-EGIQLP---KDAGLLPLLPR 373
Query: 242 IFK-LDSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLD 301
I K L L+FD P ++RD+F WL D++FARQTLAGLNP SIQLV EWPL+SKLD
Sbjct: 374 IIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLD 433
Query: 302 PEIYGPPESAFNIEMLNQKI-GSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLY 361
P +YG P S E++ +++ G++TV EA++ K+L++LDYHD+LLPYV KVR + TTLY
Sbjct: 434 PAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLY 493
Query: 362 GSRTLFFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDA 421
SRTLFFL++D TL+P+AIELT PP +KPQWK+V+ P AT W+W AK H ++HDA
Sbjct: 494 ASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDA 553
Query: 422 GYHQLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGG 481
GYHQL+SHWLRTH EPY IA NRQLSAMHPIYRLLHPHFRYT+EINA AR +L++ GG
Sbjct: 554 GYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGG 613
Query: 482 TIEFTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPF 541
IE F P KY++ELSS Y W+FD + LPADLI RGLAEED TA HG++L I DYPF
Sbjct: 614 IIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPF 673
Query: 542 ANDGLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLN 601
ANDGLILWDA+KEWVT+YV +YY D ++TSD ELQ WW+E+R +GH DKKD WP L
Sbjct: 674 ANDGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLK 733
Query: 602 TPQDLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALWKCFLE 661
T DLI +VT IAWV SGHHAAVNFGQY Y GYFPNRP+ T+ MPTEDP K F E
Sbjct: 734 TQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYE 793
Query: 662 KPEHFLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFDRFSAK 721
PE LL +PSQ QAT VM+ L++LS+HSPDEEY+G+ E SW ++PVI AAF+RF K
Sbjct: 794 SPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGK 853
Query: 722 MKNLEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
++ LE +ID RN + +LKNR GAGV YELLKP SE GVTG GVPYS+SI
Sbjct: 854 LQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896
BLAST of MC02g0081 vs. TAIR 10
Match:
AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )
HSP 1 Score: 731.9 bits (1888), Expect = 5.4e-211
Identity = 370/767 (48.24%), Postives = 503/767 (65.58%), Query Frame = 0
Query: 5 YEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKVQ 64
Y A F V FG GA+ V N ++KE +++ + + G A GP CNSWVQ P +
Sbjct: 165 YTAEFTVDSAFGSPGAITVTNKHQKEFFLESITIEGFACGPVHFPCNSWVQSQKDHPS-K 224
Query: 65 RVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGS--CSGD 124
R+ FT++ YLP TP+GL+++R EL NLRGNG GER DRIYDYDVYND+G+ S +
Sbjct: 225 RILFTNQPYLPSETPSGLRTLREKELENLRGNGKGERKLSDRIYDYDVYNDIGNPDISRE 284
Query: 125 LKRPVLGGAEHPYPRRCRTGRPHCENSPELEER-RKNESIYIPRDEAFSNIKQDAFMKDS 184
L RP LGG E PYPRRCRTGR + E R K +Y+PRDE F KQ+ F
Sbjct: 285 LARPTLGGREFPYPRRCRTGRSSTDTDMMSERRVEKPLPMYVPRDEQFEESKQNTFAACR 344
Query: 185 VLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIF-KLD 244
+ + +H ++P+L + F+NF +ID+LY + K LP I L
Sbjct: 345 LKAVLHNLIPSLKASILAE-DFANFGEIDSLYKEGLLLKLGFQDDMFKKFPLPKIVTTLQ 404
Query: 245 STGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYGP 304
+ + LR+D P+ + +DK+ WL D++FARQ +AG+NP +I+ VT +P VS LDPEIYGP
Sbjct: 405 KSSEGLLRYDTPKIVSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGP 464
Query: 305 P-ESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLF 364
SA + + ++ +TVQ+A++ +L+++DYHD+ LP++ ++ A++G Y +RT+
Sbjct: 465 GLHSALTEDHIIGQLDGLTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTIL 524
Query: 365 FLNEDETLKPLAIELTRPPMDDKPQ-WKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQL 424
FL TLKP+AIEL+ P Q KRV P AT W+W+ AKAHV ++DAG HQL
Sbjct: 525 FLTRLGTLKPIAIELSLPSQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQL 584
Query: 425 VSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFT 484
V+HWLRTH +EP+ +A +RQLSAMHPI++LL PH RYTLEINA AR LISA G IE
Sbjct: 585 VNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINAVARQTLISADGVIESC 644
Query: 485 FSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGL 544
F+ +Y +E+SS AY +W+FD++ LPADLI RG+A DPT HGLKL ++DYP+ANDGL
Sbjct: 645 FTAGQYGLEISSAAYKNKWRFDMEGLPADLIRRGMAVPDPTQPHGLKLLVEDYPYANDGL 704
Query: 545 ILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDL 604
+LW A++ WV YV YY +S+++ +D ELQ W++E VGHAD +DA WP L+T +DL
Sbjct: 705 LLWSAIQTWVRTYVERYYANSNLIQTDTELQAWYSESINVGHADHRDAEWWPKLSTVEDL 764
Query: 605 IDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTE-DPNPALWKCFLEKPEH 664
+ ++T I W+AS HAA+NFGQY Y GY PNRP + + +P E DP + F+E P+
Sbjct: 765 VSVITTIIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLIPDESDPE---FTSFIEDPQK 824
Query: 665 FLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPS-WGDDPVIKAAFDRFSAKMKN 724
+ + PS Q TK M V++ LS+HSPDEEY+G+ +PS W D I AF FSA++
Sbjct: 825 YFFSSMPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAEIGR 884
Query: 725 LEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
+E ID+RN D S +NR GAGV PYEL+ P SE GVT +GVP SVSI
Sbjct: 885 IEKEIDKRNRDPSRRNRCGAGVLPYELMAPSSEPGVTCRGVPNSVSI 926
BLAST of MC02g0081 vs. TAIR 10
Match:
AT1G17420.1 (lipoxygenase 3 )
HSP 1 Score: 721.5 bits (1861), Expect = 7.3e-208
Identity = 366/766 (47.78%), Postives = 499/766 (65.14%), Query Frame = 0
Query: 5 YEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKVQ 64
Y A F V FG GA+ V N ++KE +++ + + G A GP CNSWVQ P +
Sbjct: 159 YTAEFTVDAAFGSPGAITVMNKHQKEFFLESITIEGFALGPVHFPCNSWVQSQKDHPD-K 218
Query: 65 RVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGS--CSGD 124
R+FFT++ YLP TP+GL+ +R EL NLRG+G+G R DRIYD+DVYNDLG+ S +
Sbjct: 219 RIFFTNQPYLPNETPSGLRVLREKELKNLRGDGSGVRKLSDRIYDFDVYNDLGNPDKSSE 278
Query: 125 LKRPVLGGAEHPYPRRCRTGRPHCENSPELEER-RKNESIYIPRDEAFSNIKQDAFMKDS 184
L RP LGG E PYPRRCRTGR + + E R K +Y+PRDE F KQD F
Sbjct: 279 LSRPKLGGKEVPYPRRCRTGRQSTVSDKDAESRVEKPLPMYVPRDEQFEESKQDTFAAGR 338
Query: 185 VLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIF--KL 244
+ + +H ++P+L + F++F +ID LY + K LP + L
Sbjct: 339 LKAVLHHLIPSLKASIVAE-DFADFGEIDRLYKEGLLLKLGFQDDIFKKFPLPKVVVDTL 398
Query: 245 DSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 304
+ K L++D P+ + +DK WL D++FARQ +AG+NP +I+ V +P VS LDP+IYG
Sbjct: 399 QESTKGLLKYDTPKILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYG 458
Query: 305 PPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGTTLYGSRTLF 364
P SA + + + +VQ+A+++ +LY+LDYHD+ LP++ ++ A++G Y +RT+F
Sbjct: 459 PQHSALTDDHIIGHLDGFSVQQALEENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIF 518
Query: 365 FLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGYHQLV 424
FL TLKP+AIEL+ PP K + KRV P AT W+W+ AKAHV ++DAG HQLV
Sbjct: 519 FLTRLGTLKPVAIELSLPPHGPKHRSKRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLV 578
Query: 425 SHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTIEFTF 484
+HWLRTH +EP+ +A +RQLSAMHPI++LL PH RYTLEINA AR +LISA G IE F
Sbjct: 579 NHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLISADGVIEGGF 638
Query: 485 SPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFANDGLI 544
+ Y ME+S+ AY W+FD++ LPADLI RG+A D T HGLKL I+DYP+ANDGL+
Sbjct: 639 TAGAYGMEMSAAAYKSSWRFDMEGLPADLIRRGMAIPDATQPHGLKLLIEDYPYANDGLL 698
Query: 545 LWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTPQDLI 604
LW A++ WV YV YY + +++ +D+ELQ W++E VGHAD +DA WP L+T DL+
Sbjct: 699 LWSAIQTWVRTYVERYYPNPNLIKTDSELQSWYSESINVGHADLRDADWWPELSTVDDLV 758
Query: 605 DIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTE-DPNPALWKCFLEKPEHF 664
I+T + W+AS HAA+NFGQY Y GY PNRP + + +P E DP + F+ PE +
Sbjct: 759 SILTTLIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLIPDESDPE---YASFISHPEKY 818
Query: 665 LLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPS-WGDDPVIKAAFDRFSAKMKNL 724
+ PS Q +K M V++ LS+HSPDEEY+G+ +PS W D I AF F+A++ +
Sbjct: 819 YFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVEAFYGFAAEIGRI 878
Query: 725 EVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
E I++RNAD +NR GAGV PYELL P SE GVT +GVP SVSI
Sbjct: 879 EKEIEKRNADPDRRNRCGAGVLPYELLVPSSEPGVTCRGVPNSVSI 919
BLAST of MC02g0081 vs. TAIR 10
Match:
AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )
HSP 1 Score: 711.4 bits (1835), Expect = 7.5e-205
Identity = 358/771 (46.43%), Postives = 501/771 (64.98%), Query Frame = 0
Query: 4 IYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKV 63
++ A F V +FG+ GA++V N E+ + E+ + ++ N+W+ P+
Sbjct: 153 VFTADFTVPINFGKPGAILVTNLLSTEICLSEIIIED-STDTILFPANTWIHSKNDNPQA 212
Query: 64 QRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGS-CSGD 123
R+ F S+ LP TP G+K +R +LV++RG+G GER P++RIYDYDVYNDLG +
Sbjct: 213 -RIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDVYNDLGDPRKTE 272
Query: 124 LKRPVLGGAEHPYPRRCRTGRPHCENSPELEER-RKNESIYIPRDEAFSNIKQDAFMKDS 183
RPVLG E PYPRRCRTGRP P E R ++ E Y+PRDE F IK+D F
Sbjct: 273 RVRPVLGVPETPYPRRCRTGRPLVSKDPPCESRGKEKEEFYVPRDEVFEEIKRDTFRAGR 332
Query: 184 VLSFIHTIVPNLWVHFDD-KAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPWIFK-L 243
+ H +VP++ + F+ F DID LY + T T L + +
Sbjct: 333 FKALFHNLVPSIAAALSNLDIPFTCFSDIDNLYKSNIVLGHTEPKDTGLGGFIGGFMNGI 392
Query: 244 DSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLVSKLDPEIYG 303
+ + L++D P + D+F WL D +F RQ LAG+NP +I+L+ E P+ S LDP +YG
Sbjct: 393 LNVTETLLKYDTPAVIKWDRFAWLRDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYG 452
Query: 304 PPESAFNIEMLNQKIG--SITVQEAIQKKKLYILDYHDMLLPYVQKVRAV--EGTTLYGS 363
P ES E++ +++ T+++A+++K+L+++DYHD+LLP+V+K+ ++ + Y S
Sbjct: 453 PQESVLTEEIIAREVEHYGTTIEKALEEKRLFLVDYHDILLPFVEKINSIKEDPRKTYAS 512
Query: 364 RTLFFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQATHLWIWRFAKAHVLAHDAGY 423
RT+FF +++ L+PLAIEL+ PP + K VY AT WIW+ AKAHV ++DAG
Sbjct: 513 RTIFFYSKNGALRPLAIELSLPPTAESEN-KFVYTHGHDATTHWIWKLAKAHVCSNDAGV 572
Query: 424 HQLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNLISAGGTI 483
HQLV+HWLRTH ++EPY IATNRQLS MHP+Y+LLHPH RYTLEINA AR +LI+ GG I
Sbjct: 573 HQLVNHWLRTHASMEPYIIATNRQLSTMHPVYKLLHPHMRYTLEINARARKSLINGGGII 632
Query: 484 EFTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNIKDYPFAN 543
E F+P KY+MELSS AY W+FD++ LPADL+ RG+AEED +A G++L I DYP+A
Sbjct: 633 ESCFTPGKYAMELSSAAYKSMWRFDMEGLPADLVRRGMAEEDSSAECGVRLVIDDYPYAA 692
Query: 544 DGLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAGWPTLNTP 603
DGL++W A+K+ V YV ++Y DS +TSD ELQ WW EI+ GH DKKD WP LNT
Sbjct: 693 DGLLIWKAIKDLVESYVKHFYSDSKSITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTT 752
Query: 604 QDLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTE-DPNPALWKCFLEK 663
QDL I+TN+ W+ASG HAA+NFGQY + GY PNRP++ + +P E DP+ ++ F+
Sbjct: 753 QDLSQILTNMIWIASGQHAAINFGQYPFGGYVPNRPTLLRKLIPQETDPD---YEMFMRN 812
Query: 664 PEHFLLDAFPSQYQATKVMMVLNILSSHSPDEEYV--GKNMEPSWGDDPVIKAAFDRFSA 723
P++ L + P+Q QATKVM V LS+HSPDEEY+ + ++ W D + F++FS
Sbjct: 813 PQYSFLGSLPTQLQATKVMAVQETLSTHSPDEEYLIELREVQRHWFQDEQVVKYFNKFSE 872
Query: 724 KMKNLEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
++ +E I+ RN D LKNRTGAG+ PYELL P S GVTG+G+P S+SI
Sbjct: 873 ELVKIEKTINERNKDKKLKNRTGAGMPPYELLLPTSPHGVTGRGIPNSISI 917
BLAST of MC02g0081 vs. TAIR 10
Match:
AT1G55020.1 (lipoxygenase 1 )
HSP 1 Score: 632.5 bits (1630), Expect = 4.4e-181
Identity = 345/775 (44.52%), Postives = 466/775 (60.13%), Query Frame = 0
Query: 2 EEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDLTGL-ASGPSTITCNSWVQPPTVL 61
E ++ +F+ DFG GA ++ N + E +K + L + G CNSW+ P
Sbjct: 94 ESAFKVTFDYETDFGYPGAFLIRNSHFSEFLLKSLTLEDVPGHGRVHYICNSWIYPAKHY 153
Query: 62 PKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGTGERVPYDRIYDYDVYNDLGSCS 121
RVFF++K+YLP TPA L R ELV+LRG G GE +DR+YDY YNDLG
Sbjct: 154 -TTDRVFFSNKTYLPHETPATLLKYREEELVSLRGTGEGELKEWDRVYDYAYYNDLGVPP 213
Query: 122 GDLKRPVLGGA-EHPYPRRCRTGRPHCENSPELEER---RKNESIYIPRDEAFSNIKQDA 181
+ RPVLGG E+PYPRR RTGR + P+ E R + IY+PRDE F ++K
Sbjct: 214 KN-PRPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDIYVPRDERFGHLKMSD 273
Query: 182 FMKDSVLSFIHTIVPNLWVHFDD-KAGFSNFKDIDALYAVDGFKLPTLESSTSLKDDLPW 241
F+ ++ + I P L FDD F +F+D+ +Y +G LP S+ ++P
Sbjct: 274 FLAYALKAIAQFIQPALEAVFDDTPKEFDSFEDVLKIYE-EGIDLPNQALIDSIVKNIPL 333
Query: 242 -----IFKLDSTGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGLNPCSIQLVTEWPLV 301
IF+ D G+ L+F P+ + DK W +DE+FAR+ LAGLNP IQL+ E+P
Sbjct: 334 EMLKEIFRTD--GQKFLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNPVVIQLLKEFPPK 393
Query: 302 SKLDPEIYGPPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDMLLPYVQKVRAVEGT 361
SKLD E YG S + + +TV+EA++K++L+ILD+HD L+PY+ +V T
Sbjct: 394 SKLDSESYGNQNSTITKSHIEHNLDGLTVEEALEKERLFILDHHDTLMPYLGRVNTTT-T 453
Query: 362 TLYGSRTLFFLNEDETLKPLAIELTRP-PMDDK-PQWKRVYGPSEQATHLWIWRFAKAHV 421
Y SRTL FL +D TLKPL IEL+ P P DK VY P E + +W+ AKA V
Sbjct: 454 KTYASRTLLFLKDDGTLKPLVIELSLPHPNGDKFGAVSEVYTPGE-GVYDSLWQLAKAFV 513
Query: 422 LAHDAGYHQLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYTLEINASARTNL 481
+D+G HQL+SHW++TH ++EP+ IATNRQLS +HP+++LL PHFR T+ INA AR L
Sbjct: 514 GVNDSGNHQLISHWMQTHASIEPFVIATNRQLSVLHPVFKLLEPHFRDTMNINALARQIL 573
Query: 482 ISAGGTIEFTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEEDPTASHGLKLNI 541
I+ GG E T P KY+ME+SS Y W F QALPA+L RG+A EDP A HGL+L I
Sbjct: 574 INGGGIFEITVFPSKYAMEMSSFIYKNHWTFPDQALPAELKKRGMAVEDPEAPHGLRLRI 633
Query: 542 KDYPFANDGLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIRTVGHADKKDAAG 601
KDYP+A DGL +W A++ WV +Y+ +Y + +D ELQ WW E+R GH DKK
Sbjct: 634 KDYPYAVDGLEVWYAIESWVRDYIFLFYKIEEDIQTDTELQAWWKEVREEGHGDKKSEPW 693
Query: 602 WPTLNTPQDLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKTNMPTEDPNPALW 661
WP + T ++L++ T I WVAS HAAVNFGQY AGY PNRP+I++ MP E N +
Sbjct: 694 WPKMQTREELVESCTIIIWVASALHAAVNFGQYPVAGYLPNRPTISRQYMPKE--NTPEF 753
Query: 662 KCFLEKPEHFLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPSWGDDPVIKAAFD 721
+ + P+ L +Q Q + ++ ILS+HS DE Y+G+ W + AF+
Sbjct: 754 EELEKNPDKVFLKTITAQLQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFE 813
Query: 722 RFSAKMKNLEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKGVPYSVSI 764
+F K+K +E ID RN D +LKNRTG PY LL P SE GVTG+G+P SVSI
Sbjct: 814 KFGEKVKEIEKNIDERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| O24370 | 1.1e-280 | 60.76 | Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO... | [more] |
| R9WS04 | 7.0e-264 | 58.56 | Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=... | [more] |
| P38418 | 1.3e-257 | 56.62 | Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1 | [more] |
| P38419 | 2.1e-252 | 55.73 | Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX... | [more] |
| Q84YK8 | 4.4e-250 | 55.08 | Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022148710.1 | 0.0 | 100.00 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia] | [more] |
| XP_022153765.1 | 0.0 | 86.91 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia] | [more] |
| XP_038901038.1 | 0.0 | 80.52 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Benincasa hispida] | [more] |
| XP_022153766.1 | 0.0 | 81.15 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia] | [more] |
| XP_022958148.1 | 0.0 | 78.82 | linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1D3N9 | 0.0 | 100.00 | Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017305 PE=3 SV=1 | [more] |
| A0A6J1DHR2 | 0.0 | 86.91 | Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111021205 PE=3 SV=1 | [more] |
| A0A6J1DLP5 | 0.0 | 81.15 | Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111021206 PE=3 SV=1 | [more] |
| A0A6J1JXK9 | 0.0 | 78.69 | Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111490700 PE=3 SV=1 | [more] |
| A0A6J1H2B7 | 0.0 | 78.82 | Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459461 PE=3 SV=1 | [more] |
