MC02g0058 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g0058 vs. ExPASy Swiss-Prot
Match: Q9NWC5 (Transmembrane protein 45A OS=Homo sapiens OX=9606 GN=TMEM45A PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.6e-04
Identity = 75/289 (25.95%), Postives = 106/289 (36.68%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFPTRKLKYLEPLLIMGG 60
           MG   GH  PG  F IIGLW    +I          T    S     +L+ LE + I+G 
Sbjct: 1   MGNFRGHALPGTFFFIIGLWWCTKSILKYICKKQKRTCYLGSKTLFYRLEILEGITIVGM 60

Query: 61  ATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAY 120
           A   +A E FI P   H    D     ++     H      FF       +L        
Sbjct: 61  ALTGMAGEQFI-PGGPHLMLYDYKQGHWNQLLGWHHFTMYFFFGLLGVADILCFTISSLP 120

Query: 121 FELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLV 180
             LT+L+ + A   E  +F+ H+      +   H LL L+V L+     L   +  + L+
Sbjct: 121 VSLTKLMLSNALFVEAFIFYNHTHGREMLDIFVHQLLVLVVFLTGLVAFLEFLVRNNVLL 180

Query: 181 AFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINI 240
             +RS  IL QG W   +GF L+  S  P                  D   H     + I
Sbjct: 181 ELLRSSLILLQGSWFFQIGFVLYPPSGGP----------------AWDLMDHENILFLTI 240

Query: 241 QFSW---VLIALTILTLTFYLVVVKVYETPKAAEYFSLMTNEEEEEEKE 287
            F W   V I +  +   F   +VK       +    L+ N E E+E E
Sbjct: 241 CFCWHYAVTIVIVGMNYAFITWLVKSRLKRLCSSEVGLLKNAEREQESE 272

BLAST of MC02g0058 vs. NCBI nr
Match: XP_008449792.1 (PREDICTED: transmembrane protein 45A [Cucumis melo])

HSP 1 Score: 478 bits (1231), Expect = 1.88e-167
Identity = 248/333 (74.47%), Postives = 282/333 (84.68%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNN----TIMFPSWFPTRKLKYLEPLL 60
           MGTLVGHVAPGFGF IIGLWHLFNNIKI+A N NNN    T +F SWFPT + KYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNNNNHPNTAIFLSWFPTSRFKYLEPFL 60

Query: 61  IMGGATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLR 120
           IM GA+AS+AMELFIGPQRH PFDTDGTIPSYHLHN EHSNIS+TFFVFAAFC++LDR+R
Sbjct: 61  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRVR 120

Query: 121 PKAYFELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPV 180
           PKAYFELTQ VG VAFGQELLLFHLHSADHMGPE QYHLLLQLLV++S ATTLLGIALP 
Sbjct: 121 PKAYFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPN 180

Query: 181 SFLVAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKA 240
           SFLVAFVRS +I+FQGLWL+LMGFALWT SFIPKGCF+HLEDGH+VVRC GD  LHRAK+
Sbjct: 181 SFLVAFVRSLSIVFQGLWLILMGFALWTPSFIPKGCFMHLEDGHKVVRCAGDEPLHRAKS 240

Query: 241 LINIQFSWVLIALTILTLTFYLVVVKVYE--TPKA--AEYFSLMTNEEEEEEKEAV-ARS 300
           LINIQFSW+LIALTIL L+FYLV++K+Y+  TPK    +YFSL+TN++EE    +   ++
Sbjct: 241 LINIQFSWLLIALTILGLSFYLVLLKLYQQQTPKINPVQYFSLITNDDEEAAATSTPTKT 300

Query: 301 SAYDLESQDKDKHGVSRSFIQMGQKLTDMSMER 324
           ++YD+         VS+SFIQMG KLTD  MER
Sbjct: 301 TSYDV---------VSKSFIQMGHKLTDTDMER 324

BLAST of MC02g0058 vs. NCBI nr
Match: XP_004142158.1 (transmembrane protein 45A [Cucumis sativus] >KGN54083.1 hypothetical protein Csa_018130 [Cucumis sativus])

HSP 1 Score: 473 bits (1217), Expect = 3.03e-165
Identity = 246/336 (73.21%), Postives = 282/336 (83.93%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNN-NTIMFPSWFPTRKLKYLEPLLIMG 60
           MGTLVGHVAPGFGF IIGLWHLFNNIKI+A N N  NT +F SWFPT + KYLEP LIM 
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNPPNTAIFLSWFPTSRFKYLEPFLIMV 60

Query: 61  GATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKA 120
           GA+AS+AMELFIGPQRH PFD DGTIPSYHLHN EHSNIS+TFFVFAAFC++LD++RPKA
Sbjct: 61  GASASIAMELFIGPQRHQPFDIDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDQVRPKA 120

Query: 121 YFELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFL 180
           YFELTQ VG VAFGQELLLFHLHSADHMGPE QYHLLLQLLVV+S ATTLLGIALP SFL
Sbjct: 121 YFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVVVSFATTLLGIALPNSFL 180

Query: 181 VAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALIN 240
           +AFVRS +I+FQGLWL+LMGFALWT SFIPKGCF+HLEDGH+VVRC GD  LHRAK+LIN
Sbjct: 181 LAFVRSLSIVFQGLWLILMGFALWTPSFIPKGCFMHLEDGHKVVRCAGDEPLHRAKSLIN 240

Query: 241 IQFSWVLIALTILTLTFYLVVVKVYE--TP-----KAAEYFSLMTNEEEEEEKEAVA--- 300
           IQFSW+LIALTIL+L+FYLV++K+Y+  TP        +YFSL+TN++EEE+ EA     
Sbjct: 241 IQFSWLLIALTILSLSFYLVLLKLYQQQTPDPKKNNPVQYFSLITNDDEEEDAEAATSTP 300

Query: 301 -RSSAYDLESQDKDKHGVSRSFIQMGQKLTDMSMER 324
            ++S+Y++ S       V +SFIQMG KL D  MER
Sbjct: 301 TKTSSYNVVS-------VPKSFIQMGHKLADTDMER 329

BLAST of MC02g0058 vs. NCBI nr
Match: XP_038901896.1 (transmembrane protein 45B-like [Benincasa hispida])

HSP 1 Score: 469 bits (1206), Expect = 1.04e-163
Identity = 244/329 (74.16%), Postives = 275/329 (83.59%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNN-NTIMFPSWFPTRKLKYLEPLLIMG 60
           MGTLVGHVAPGFGF IIGLWHLFNN KI+ARN +  NT+ F SWFPT + KYLEP LIM 
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60

Query: 61  GATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKA 120
           GA+AS+AMELFIGPQ+H PFDTDGTIPSYHLHN EHSNIS+TFFVFAAFC++LDRLRPKA
Sbjct: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120

Query: 121 YFELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFL 180
           YFELTQ VG VAFGQELLLFHLHSADHMGPE QYHLLLQLLV +S ATTLLGIA P SFL
Sbjct: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180

Query: 181 VAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALIN 240
           VAFVRS +I+FQGLWLMLMGFALWT SFIPKGCF+HLEDGH+VVRC G+  L RAKALIN
Sbjct: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240

Query: 241 IQFSWVLIALTILTLTFYLVVVKVY--ETPK-AAEYFSLMTNEEEEE-EKEAVARSSAYD 300
           +QFSW+LIALTI  L+F+L+++K+Y  ETPK AA YFSLMT++EE+  E  A  + + YD
Sbjct: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD 300

Query: 301 LESQDKDKHGVSRSFIQMGQKLTDMSMER 324
           +          SRSFIQMG K T+  MER
Sbjct: 301 V---------ASRSFIQMGHKFTETDMER 320

BLAST of MC02g0058 vs. NCBI nr
Match: XP_022153784.1 (uncharacterized protein LOC111021221, partial [Momordica charantia])

HSP 1 Score: 462 bits (1190), Expect = 1.74e-162
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 82  DGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAYFELTQLVGAVAFGQELLLFHL 141
           DGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAYFELTQLVGAVAFGQELLLFHL
Sbjct: 1   DGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAYFELTQLVGAVAFGQELLLFHL 60

Query: 142 HSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLVAFVRSFTILFQGLWLMLMGFA 201
           HSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLVAFVRSFTILFQGLWLMLMGFA
Sbjct: 61  HSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLVAFVRSFTILFQGLWLMLMGFA 120

Query: 202 LWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINIQFSWVLIALTILTLTFYLVVV 261
           LWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINIQFSWVLIALTILTLTFYLVVV
Sbjct: 121 LWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINIQFSWVLIALTILTLTFYLVVV 180

Query: 262 KVYETPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQDKDKHGVSRSFIQMGQKLTDMS 321
           KVYETPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQDKDKHGVSRSFIQMGQKLTDMS
Sbjct: 181 KVYETPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQDKDKHGVSRSFIQMGQKLTDMS 240

Query: 322 MER 324
           MER
Sbjct: 241 MER 243

BLAST of MC02g0058 vs. NCBI nr
Match: KAG7010234.1 (hypothetical protein SDJN02_27026, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 464 bits (1195), Expect = 3.42e-162
Identity = 238/325 (73.23%), Postives = 271/325 (83.38%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFPTRKLKYLEPLLIMGG 60
           MGTLVGHVAPGFGF IIGLWHLFNNIKI+ARN   N+ +F SWFPT + KYLEPLLIM G
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEARNNPPNSPIFLSWFPTSRFKYLEPLLIMLG 60

Query: 61  ATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAY 120
           A+ASVAMELFIGPQRHHPFDTDGTIPS HLHN+EHSNIS+TFFVFAAF ++LDRLRPKAY
Sbjct: 61  ASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLRPKAY 120

Query: 121 FELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLV 180
           FELTQ +GAVAFGQELLLFHLHSADH GPE QYH+LLQLLV+LSLATTLLGIALP SFLV
Sbjct: 121 FELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPTSFLV 180

Query: 181 AFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINI 240
           AF+RSF+I+FQG+WLMLMGF LWT S I KGCF+HLEDGH+VVRC G+A LHRAK+LINI
Sbjct: 181 AFIRSFSIIFQGVWLMLMGFTLWTPSLISKGCFMHLEDGHKVVRCGGEAPLHRAKSLINI 240

Query: 241 QFSWVLIALTILTLTFYLVVVKVYE-TPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQ 300
           QFSW+LIALTI  L+F+L ++K+Y+ TPK  +Y SL+TNEEEEE    +   +       
Sbjct: 241 QFSWLLIALTIFALSFHLFLLKLYQQTPKNPQYISLVTNEEEEEPSNNIDTRA------- 300

Query: 301 DKDKHGVSRSFIQMGQKLTDMSMER 324
                   RSFI+MG K T+M M R
Sbjct: 301 --------RSFIEMGHKFTEMGMGR 310

BLAST of MC02g0058 vs. ExPASy TrEMBL
Match: A0A1S3BMV5 (transmembrane protein 45A OS=Cucumis melo OX=3656 GN=LOC103491576 PE=3 SV=1)

HSP 1 Score: 478 bits (1231), Expect = 9.09e-168
Identity = 248/333 (74.47%), Postives = 282/333 (84.68%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNN----TIMFPSWFPTRKLKYLEPLL 60
           MGTLVGHVAPGFGF IIGLWHLFNNIKI+A N NNN    T +F SWFPT + KYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNNNNHPNTAIFLSWFPTSRFKYLEPFL 60

Query: 61  IMGGATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLR 120
           IM GA+AS+AMELFIGPQRH PFDTDGTIPSYHLHN EHSNIS+TFFVFAAFC++LDR+R
Sbjct: 61  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRVR 120

Query: 121 PKAYFELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPV 180
           PKAYFELTQ VG VAFGQELLLFHLHSADHMGPE QYHLLLQLLV++S ATTLLGIALP 
Sbjct: 121 PKAYFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPN 180

Query: 181 SFLVAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKA 240
           SFLVAFVRS +I+FQGLWL+LMGFALWT SFIPKGCF+HLEDGH+VVRC GD  LHRAK+
Sbjct: 181 SFLVAFVRSLSIVFQGLWLILMGFALWTPSFIPKGCFMHLEDGHKVVRCAGDEPLHRAKS 240

Query: 241 LINIQFSWVLIALTILTLTFYLVVVKVYE--TPKA--AEYFSLMTNEEEEEEKEAV-ARS 300
           LINIQFSW+LIALTIL L+FYLV++K+Y+  TPK    +YFSL+TN++EE    +   ++
Sbjct: 241 LINIQFSWLLIALTILGLSFYLVLLKLYQQQTPKINPVQYFSLITNDDEEAAATSTPTKT 300

Query: 301 SAYDLESQDKDKHGVSRSFIQMGQKLTDMSMER 324
           ++YD+         VS+SFIQMG KLTD  MER
Sbjct: 301 TSYDV---------VSKSFIQMGHKLTDTDMER 324

BLAST of MC02g0058 vs. ExPASy TrEMBL
Match: A0A0A0KX38 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G280600 PE=3 SV=1)

HSP 1 Score: 473 bits (1217), Expect = 1.47e-165
Identity = 246/336 (73.21%), Postives = 282/336 (83.93%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNN-NTIMFPSWFPTRKLKYLEPLLIMG 60
           MGTLVGHVAPGFGF IIGLWHLFNNIKI+A N N  NT +F SWFPT + KYLEP LIM 
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNPPNTAIFLSWFPTSRFKYLEPFLIMV 60

Query: 61  GATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKA 120
           GA+AS+AMELFIGPQRH PFD DGTIPSYHLHN EHSNIS+TFFVFAAFC++LD++RPKA
Sbjct: 61  GASASIAMELFIGPQRHQPFDIDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDQVRPKA 120

Query: 121 YFELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFL 180
           YFELTQ VG VAFGQELLLFHLHSADHMGPE QYHLLLQLLVV+S ATTLLGIALP SFL
Sbjct: 121 YFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVVVSFATTLLGIALPNSFL 180

Query: 181 VAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALIN 240
           +AFVRS +I+FQGLWL+LMGFALWT SFIPKGCF+HLEDGH+VVRC GD  LHRAK+LIN
Sbjct: 181 LAFVRSLSIVFQGLWLILMGFALWTPSFIPKGCFMHLEDGHKVVRCAGDEPLHRAKSLIN 240

Query: 241 IQFSWVLIALTILTLTFYLVVVKVYE--TP-----KAAEYFSLMTNEEEEEEKEAVA--- 300
           IQFSW+LIALTIL+L+FYLV++K+Y+  TP        +YFSL+TN++EEE+ EA     
Sbjct: 241 IQFSWLLIALTILSLSFYLVLLKLYQQQTPDPKKNNPVQYFSLITNDDEEEDAEAATSTP 300

Query: 301 -RSSAYDLESQDKDKHGVSRSFIQMGQKLTDMSMER 324
            ++S+Y++ S       V +SFIQMG KL D  MER
Sbjct: 301 TKTSSYNVVS-------VPKSFIQMGHKLADTDMER 329

BLAST of MC02g0058 vs. ExPASy TrEMBL
Match: A0A6J1DK38 (uncharacterized protein LOC111021221 OS=Momordica charantia OX=3673 GN=LOC111021221 PE=3 SV=1)

HSP 1 Score: 462 bits (1190), Expect = 8.44e-163
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 82  DGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAYFELTQLVGAVAFGQELLLFHL 141
           DGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAYFELTQLVGAVAFGQELLLFHL
Sbjct: 1   DGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAYFELTQLVGAVAFGQELLLFHL 60

Query: 142 HSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLVAFVRSFTILFQGLWLMLMGFA 201
           HSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLVAFVRSFTILFQGLWLMLMGFA
Sbjct: 61  HSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLVAFVRSFTILFQGLWLMLMGFA 120

Query: 202 LWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINIQFSWVLIALTILTLTFYLVVV 261
           LWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINIQFSWVLIALTILTLTFYLVVV
Sbjct: 121 LWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINIQFSWVLIALTILTLTFYLVVV 180

Query: 262 KVYETPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQDKDKHGVSRSFIQMGQKLTDMS 321
           KVYETPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQDKDKHGVSRSFIQMGQKLTDMS
Sbjct: 181 KVYETPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQDKDKHGVSRSFIQMGQKLTDMS 240

Query: 322 MER 324
           MER
Sbjct: 241 MER 243

BLAST of MC02g0058 vs. ExPASy TrEMBL
Match: A0A6J1JGR5 (transmembrane protein 45A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484363 PE=3 SV=1)

HSP 1 Score: 454 bits (1169), Expect = 1.50e-158
Identity = 232/325 (71.38%), Postives = 270/325 (83.08%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFPTRKLKYLEPLLIMGG 60
           MGTLVGHVAPGFGF IIGLWHLFNN+KI+AR+   N+ +F SWFPT + KYLEPLLIM G
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNMKIEARSSPPNSPVFLSWFPTSRFKYLEPLLIMLG 60

Query: 61  ATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAY 120
           A+ASVAMELFIGPQRHHPFDTDGTIPS HLHN+EHSNIS+TFFVFAAF ++LDRLRPKAY
Sbjct: 61  ASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLRPKAY 120

Query: 121 FELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLV 180
           FELTQ +GAVAFGQELLLFHLHSADH GPE QYH+LLQLLV+LSLATTLLGIALP SFLV
Sbjct: 121 FELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPTSFLV 180

Query: 181 AFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINI 240
           AF+RSF+I+FQG+WLMLMGF LWT S I KGCF+HLEDGH+VVRC G+A LHRAK+L+NI
Sbjct: 181 AFIRSFSIIFQGVWLMLMGFTLWTPSLISKGCFMHLEDGHKVVRCGGEAPLHRAKSLVNI 240

Query: 241 QFSWVLIALTILTLTFYLVVVKVYE-TPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQ 300
           QFSW+LIALTI  L+ +L ++K+Y+ TPK  +Y SL+T+E EEE  + +   +       
Sbjct: 241 QFSWLLIALTIFALSLHLFLLKLYQQTPKNPQYISLITSEVEEETSKNIDTRA------- 300

Query: 301 DKDKHGVSRSFIQMGQKLTDMSMER 324
                   RSFI+MG K T+M M R
Sbjct: 301 --------RSFIEMGHKFTEMGMGR 310

BLAST of MC02g0058 vs. ExPASy TrEMBL
Match: A0A6J1FXU7 (transmembrane protein 45A-like OS=Cucurbita moschata OX=3662 GN=LOC111448740 PE=3 SV=1)

HSP 1 Score: 452 bits (1163), Expect = 1.10e-157
Identity = 231/321 (71.96%), Postives = 266/321 (82.87%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFPTRKLKYLEPLLIMGG 60
           MGTLVGHVAPGFGF IIGLWHLFNNIKI+AR    N+ +F SWFPT + KYLEPLLIM G
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEARTNPPNSPIFLSWFPTSRFKYLEPLLIMLG 60

Query: 61  ATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAY 120
           A+ASVAMELFIGPQRHHPFDTDGTIPS HLHN+EHSNIS+TFFVFAAF ++LDRLRPKAY
Sbjct: 61  ASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLRPKAY 120

Query: 121 FELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFLV 180
           FELTQ +GAVAFGQELLLFHLHSADH GPE QYH+LLQLLV+LSLATTLLGIALP SFLV
Sbjct: 121 FELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPTSFLV 180

Query: 181 AFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALINI 240
           AF+RSF+I+FQG+WLMLMGF LWT S I KGCF+HLEDGH+VVRC G+A  HRAK+LINI
Sbjct: 181 AFIRSFSIIFQGVWLMLMGFTLWTPSLISKGCFMHLEDGHKVVRCGGEAPFHRAKSLINI 240

Query: 241 QFSWVLIALTILTLTFYLVVVKVYE-TPKAAEYFSLMTNEEEEEEKEAVARSSAYDLESQ 300
           QFSW+L ALTI  L+F+L ++K+Y+ TPK  +Y SL+TNEEEEE  + +   +       
Sbjct: 241 QFSWLLTALTIFALSFHLFLLKLYQQTPKNPQYISLVTNEEEEETSKNIDTRA------- 300

Query: 301 DKDKHGVSRSFIQMGQKLTDM 320
                   RSFI+MG K  ++
Sbjct: 301 --------RSFIEMGHKFKEI 306

BLAST of MC02g0058 vs. TAIR 10
Match: AT1G55230.1 (Family of unknown function (DUF716) )

HSP 1 Score: 318.9 bits (816), Expect = 4.6e-87
Identity = 164/305 (53.77%), Postives = 219/305 (71.80%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFPTRKLKYLEPLLIMGG 60
           MGTLVGHVAPGF F  +GLWHLFNNIK+     N NT     WFPT KL++LE  +IM  
Sbjct: 1   MGTLVGHVAPGFAFFALGLWHLFNNIKLFCL--NPNTFTSSPWFPTSKLRHLELYIIMVC 60

Query: 61  ATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAY 120
           ++ S+A ELFIGP++H PFD+DGTIPS HLHN EHS+IS++F V+A F ++LD+ R ++ 
Sbjct: 61  SSISIASELFIGPKKHQPFDSDGTIPSNHLHNFEHSSISMSFLVYAIFAVVLDQERRRSN 120

Query: 121 FE--LTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSF 180
               LT LV + AF Q+L LFHLHSADHMG E QYH LLQL++ +S  TTL+GI LP SF
Sbjct: 121 ISHGLTMLVASSAFAQQLFLFHLHSADHMGIEGQYHKLLQLIIFVSFLTTLIGITLPKSF 180

Query: 181 LVAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALI 240
           LV+FVRS +I FQG+W ++MG+ LWT S IPKGCF+H E+GH+V++C  D ++HRAK+L+
Sbjct: 181 LVSFVRSSSITFQGVWFVVMGYMLWTPSLIPKGCFLHEEEGHQVIKCSSDKAIHRAKSLV 240

Query: 241 NIQFSWVLIALTILTLTFYLVVVKVYETPKAAEYFSLMTNEEEEEEKEAVARSSAYDLES 300
           NI+FSW  + +TI  ++ +L++  +Y   + AEY  L+T ++ EE  E        D+ES
Sbjct: 241 NIEFSWFFVGITIFVMSLFLILSGLY--GENAEYSILVTKDKGEESGE-----EKQDIES 296

Query: 301 QDKDK 304
             K K
Sbjct: 301 LKKSK 296

BLAST of MC02g0058 vs. TAIR 10
Match: AT1G55240.1 (Family of unknown function (DUF716) )

HSP 1 Score: 308.9 bits (790), Expect = 4.8e-84
Identity = 161/317 (50.79%), Postives = 220/317 (69.40%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFPTRKLKYLEPLLIMGG 60
           MG+ +GHV PGF F+ +GLWHLFNNIK+     + NT    +WFP  KL++LE  LIM  
Sbjct: 1   MGSFLGHVLPGFAFVALGLWHLFNNIKLFCL--HPNTFSSSTWFPISKLRHLELYLIMLS 60

Query: 61  ATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKAY 120
           +TAS++MELFIGP+RHHPFD DGTIPS HLHN EHS+IS++F V+A   ++LDR RP+A 
Sbjct: 61  STASISMELFIGPRRHHPFDVDGTIPSNHLHNFEHSSISMSFLVYAVLALVLDRARPRAA 120

Query: 121 FE--LTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSF 180
               LT L  A AF Q+LLLFH HS DHMG E QYH++LQ+++ +SL TT++GI LP SF
Sbjct: 121 ASEGLTMLAAAAAFSQQLLLFHFHSTDHMGVEGQYHVILQVVIFVSLLTTIMGIFLPKSF 180

Query: 181 LVAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALI 240
           LV+ VRS +I FQG+WL+++G  L+T S IPKGC+IH E  H +V+C  + +LHRAK+L+
Sbjct: 181 LVSLVRSSSIAFQGVWLIVIGCMLYTPSLIPKGCYIHDEGRHIIVKCSTEEALHRAKSLV 240

Query: 241 NIQFSWVLIALTILTLTFYLVVVKVYETPKAAEYFSLMTNEEEEEEKEAVARSSAYDLES 300
           N++FSW+ +  T+  +T YL++ +VY   +  EY SL TN +  ++ E          E 
Sbjct: 241 NLEFSWLFVTNTLFVVTLYLILDRVY--GENVEYSSLTTNNQTYQDDE----------EQ 300

Query: 301 QDKDKHGVSRSFIQMGQ 316
             + K     SF+QMG+
Sbjct: 301 HFESKSTQKTSFVQMGK 303

BLAST of MC02g0058 vs. TAIR 10
Match: AT5G19870.1 (Family of unknown function (DUF716) )

HSP 1 Score: 297.0 bits (759), Expect = 1.9e-80
Identity = 148/280 (52.86%), Postives = 198/280 (70.71%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFPTRKLKYLEPLLIMGG 60
           MGTLVGH+ PGF F  +GLWHLFN+IKI +   +  + + P WFP  K +YLEPL+I+  
Sbjct: 1   MGTLVGHILPGFFFFALGLWHLFNHIKIFSL--HPKSYIAPLWFPLSKPRYLEPLVIIIA 60

Query: 61  ATASVAMELFIGPQRHHPFD-TDGTIPSYHLHNLEHSNISITFFVFAAFCILLDRLRPKA 120
           ++ S+++ELF+G + H PFD  DGTIPS HLHN EHS IS TF  +AAF I+ D+ RP A
Sbjct: 61  SSLSISVELFLGQKNHQPFDPNDGTIPSNHLHNFEHSCISFTFITYAAFAIVFDKTRPIA 120

Query: 121 YFELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFL 180
           +  L  L+ A+AF Q+L LFH HS+DH G E  YHLLLQL+V +SL TTLLGIALP SF+
Sbjct: 121 HRSLINLIAALAFAQQLFLFHFHSSDHTGVEGHYHLLLQLVVFVSLVTTLLGIALPSSFI 180

Query: 181 VAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRAKALIN 240
           ++FVRS ++ FQG+WLM M   LWT S +PK CF+H+E+G   +RC    +LHRA +L+N
Sbjct: 181 LSFVRSLSVSFQGIWLMSMACMLWTPSLVPKDCFLHIEEGKHTIRCSDVKALHRAISLVN 240

Query: 241 IQFSWVLIALTILTLTFYLVVVKVYETPKAAEYFSLMTNE 280
           IQFSW L+ +TI  + FY+ + ++Y   +  EY  L T +
Sbjct: 241 IQFSWFLVIITIFAMWFYIFLQRIY--GEKIEYSQLRTKD 276

BLAST of MC02g0058 vs. TAIR 10
Match: AT1G49470.1 (Family of unknown function (DUF716) )

HSP 1 Score: 154.5 bits (389), Expect = 1.5e-37
Identity = 93/269 (34.57%), Postives = 134/269 (49.81%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFPTRKLKYLEPLLIMGG 60
           MGT +GH  PG    ++GLWHLFN I+     G         WFP  KLK+LE +LI+  
Sbjct: 1   MGTFLGHFVPGLSLALLGLWHLFNTIRSYCLKG-PEAFSAKFWFPFPKLKHLELILILFF 60

Query: 61  ATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLD-RLRPKA 120
           +  S+ +     P     F+     P     NLEH+++ +   +FA F +  +  L    
Sbjct: 61  SFLSITLLTLDFPN----FNFSSLKPD----NLEHASMFLHLIIFACFALFCELTLCSDL 120

Query: 121 YFELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIALPVSFL 180
           +  L  ++ A  F QEL L H HS DH G E  YH LLQL+  +S ++ L   + P SF 
Sbjct: 121 FSGLIGVLSASVFAQELFLLHFHSTDHSGLEGHYHFLLQLIAFVSFSSALASASFPKSFS 180

Query: 181 VAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHE----------VVRCIGDA 240
            A     +++FQG W + MGF LW   ++P+GC  ++    +           V C    
Sbjct: 181 AALFLPISVMFQGCWFLNMGFMLWIPEYVPRGCVSNMSTSTDNNRRSVYHSGAVACESPG 240

Query: 241 SLHRAKALINIQFSWVLIALTILTLTFYL 259
           +  RAKAL N+QFSW+L A+ I+T    L
Sbjct: 241 AEIRAKALANLQFSWMLSAILIITCALCL 260

BLAST of MC02g0058 vs. TAIR 10
Match: AT1G32120.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: membrane; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 4 anthesis, C globular stage, F mature embryo stage, petal differentiation and expansion stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Aminotransferase-like, plant mobile domain (InterPro:IPR019557), Protein of unknown function DUF716 (InterPro:IPR006904); BEST Arabidopsis thaliana protein match is: Aminotransferase-like, plant mobile domain family protein (TAIR:AT1G51538.1); Has 16736 Blast hits to 9656 proteins in 576 species: Archae - 4; Bacteria - 1182; Metazoa - 7098; Fungi - 2631; Plants - 1178; Viruses - 174; Other Eukaryotes - 4469 (source: NCBI BLink). )

HSP 1 Score: 104.0 bits (258), Expect = 2.3e-22
Identity = 76/264 (28.79%), Postives = 124/264 (46.97%), Query Frame = 0

Query: 1    MGTLVGHVAPGFGFLIIGLWHLFNNIKIQARNGNNNTIMFPSWFP----TRKLKYLEPLL 60
            MG+  GH  PG  FL++G+WH+++++     N ++  +    W P      ++KYLE  +
Sbjct: 943  MGSFKGHALPGTLFLVVGVWHIWSSVVRYISNPSSFRVRV--WHPVPGFNDRIKYLELYV 1002

Query: 61   IMGGATASVAMELFIGPQRHHPFDTDGTIPSYHLHNLEHSNISITFFVFAAFCILLD--R 120
            +  G+   + +E       H  F  +G +   H+++ EHS + + FF+     +L +  R
Sbjct: 1003 VTIGSFIDLCIEFLY--STHLKFFVNGVLNPSHMNDFEHSGMLLMFFILGFIALLSEKTR 1062

Query: 121  LRPKAYFELTQLVGAVAFGQELLLFHLHSADHMGPEAQYHLLLQLLVVLSLATTLLGIAL 180
            L P    E   L+ A AF  E LLF  HS  H G E  YHLLL  L+ L + +++ G   
Sbjct: 1063 LLPLPQ-EALCLIAATAFTAECLLFFFHSTSHKGLEGYYHLLLVFLIGLCVISSIAGAIC 1122

Query: 181  PVSFLVAFVRSFTILFQGLWLMLMGFALWTRSFIPKGCFIHLEDGHEVVRCIGDASLHRA 240
            P SF V       +  QGLW     F L+    +P+GC +        V C    S    
Sbjct: 1123 PTSFPVDLCNGIAMTLQGLWFYQTAFTLY-GPMMPQGCSLK----QNSVVCRSVDSEVSG 1182

Query: 241  KALINIQFSWVLIALTILTLTFYL 259
            + L N Q   +++A+ +  +  Y+
Sbjct: 1183 EFLANFQLFSLVLAVLVCVVGSYV 1196