MC02g0023 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0023
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	GDSL esterase/lipase
Location	MC02: 268829 .. 270736 (-)
RNA-Seq Expression	MC02g0023
Synteny	MC02g0023

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAAGTGGGTACCAAATGGTTAGTAATTATGGAAATAATGATTTATTTTGTACCAAATTAGTGGTATGAGTTTCACATTCGTATGAAGACAACAGTTGAACAGAACACCAACCCTTCTTCTACGTCTTCTACTTCCAAGTAGCGACACAGAAACGGAAACAGAAGGAAGAACTGAAGAATCTGAAATTAAAGGAATTATATAATATGGGCGTGGAGGAGCAGGTGTTGATTGGTGTGTTATTGGGGATGGTGGCAGTTGCAGTTGGAGTTGCAGATCCACCTCAAGTATCCAATGTCACCGCCATGTACTTGTTGGGAGATTCTTCTGTCGATTGCGGCTTAAGCACTCTCTATTATCCTCTTCTCCACCGCAATTTCTCTCTGTTTCCTTGTGAAGCCGACGCCGACGCCGACGCCTCTACTCTTCTCCCTCACTTTCTTGGTACTCTCTTTTCTCTTTCTTGTTCGCATTTCTTGATAATAATACTCTGCAATTGCTTCAAGAAATGCCATCCTATGATTACTGTTTCTTGTACATCGATCTTCTGATCAACTGATCCCAAATTCAAAATGGACGAAAATCTACCGTATTCTTGCAGCTAAGAAGATAGGCGTGCCATACTCTCAACCCCTGTACACTCAGAACGGATCCATTGAAGCGATCCTAAATGGCCTCAACTTCGGTTCGCCACAAGCAACGATTATGAGCAGCAGCCGTAGCTATCAGTCCCTCAACCAACAGCTTCGTCAGGCACTCGACGCCATTCAGATGTTGCGCCTGCATCTCGGCCAAGACACTGCTCACCGTTTCATCCAATCATCCGTCTTCTACCTCTCCTTCGGCGAGATCGATTTCATTAATCTCTACCTCCTAAACTCGACAGGAAAGCAATTGAAGTACAAGGGCAAGGAATTTGGTCGGCTGCTGGTATCCCAGATGGTGATCGCCATAAGAAACCTGGAGGAAGCAGGCGCGAGGAAGATCATATGCATGGGAATACTGCCATTGGGGTGCACTCCTCGAGTGCTGTCTCTGTGGCGCAACTCCCCCGGGGCTGTATACGACGAGAAGGGATGTGTAAAAGAGATGAACGAGTTGGTTGCAGAGTACAACAGAGGGATGGAGAAGCAGATAGTGAAGTTCGGCAAAGGTCGGGTGGTATTCTGCGACGCGTACAAAGGGATGATGGAGATCGTGGGGAGCCCAAGGCGATACGGTATGGAGATCTCGTTTGCGTCTGCGTCTGTCGATGAACCTAAAGAATATTATGTGAAATGAATGATATGTGTATTGTGTAGGATTTGAGGAGTCGAAGAGTGCGTGTTGTGGGCTGGGTTGGTACAACGCGAGCATAGGATGCGTGGCAATGGAGATGGCGTGCAGCCGAGTGGGGAGGAGCGTGTGGTGGGACTTGTACAATCCGACGGGGGCTGTCAATTCGCTGCTTGCGGATTCTGCGTGGTCTGATCAGCCCTTCTCCAGCTTCTGCCATCCATCCACCGTTCAGGACTTGGTATGGCCTTAAATCAATTCATTCATTTACGTGGGTTATTTGGTTGTTTCCGAAAACTCATTTGATCAATTGCAATTTCTCTCTTTGCAAGATAAATGAAACTTCAGTTCATACAAATGAAGGAAAAAAGGTTGCATATCAATCACTAAGTAGATATCTCTGTTTGTTTTTTTAGAAATTGGGAATAGAGAATATAAGAAAATGAATTATAACTCCTTATTGCTAAGTGGTTAAGTTGGGCTCTATATGTTGTCAACAAAAGAAAAGATTAATGGTAATAATTGTGTCCAGCTTCAGTGAAATAAAACTGGGTTATGCTACAAACTTATAGTTATACTAGTTACTTTGTTACTTGTAAGCTGTCATATTTTTAAATATAACATAAATAAATC

mRNA sequence

AGAAAAGTGGGTACCAAATGGTTAGTAATTATGGAAATAATGATTTATTTTGTACCAAATTAGTGGTATGAGTTTCACATTCGTATGAAGACAACAGTTGAACAGAACACCAACCCTTCTTCTACGTCTTCTACTTCCAAGTAGCGACACAGAAACGGAAACAGAAGGAAGAACTGAAGAATCTGAAATTAAAGGAATTATATAATATGGGCGTGGAGGAGCAGGTGTTGATTGGTGTGTTATTGGGGATGGTGGCAGTTGCAGTTGGAGTTGCAGATCCACCTCAAGTATCCAATGTCACCGCCATGTACTTGTTGGGAGATTCTTCTGTCGATTGCGGCTTAAGCACTCTCTATTATCCTCTTCTCCACCGCAATTTCTCTCTGTTTCCTTGTGAAGCCGACGCCGACGCCGACGCCTCTACTCTTCTCCCTCACTTTCTTGCTAAGAAGATAGGCGTGCCATACTCTCAACCCCTGTACACTCAGAACGGATCCATTGAAGCGATCCTAAATGGCCTCAACTTCGGTTCGCCACAAGCAACGATTATGAGCAGCAGCCGTAGCTATCAGTCCCTCAACCAACAGCTTCGTCAGGCACTCGACGCCATTCAGATGTTGCGCCTGCATCTCGGCCAAGACACTGCTCACCGTTTCATCCAATCATCCGTCTTCTACCTCTCCTTCGGCGAGATCGATTTCATTAATCTCTACCTCCTAAACTCGACAGGAAAGCAATTGAAGTACAAGGGCAAGGAATTTGGTCGGCTGCTGGTATCCCAGATGGTGATCGCCATAAGAAACCTGGAGGAAGCAGGCGCGAGGAAGATCATATGCATGGGAATACTGCCATTGGGGTGCACTCCTCGAGTGCTGTCTCTGTGGCGCAACTCCCCCGGGGCTGTATACGACGAGAAGGGATGTGTAAAAGAGATGAACGAGTTGGTTGCAGAGTACAACAGAGGGATGGAGAAGCAGATAGTGAAGTTCGGCAAAGGTCGGGTGGTATTCTGCGACGCGTACAAAGGGATGATGGAGATCGTGGGGAGCCCAAGGCGATACGGATTTGAGGAGTCGAAGAGTGCGTGTTGTGGGCTGGGTTGGTACAACGCGAGCATAGGATGCGTGGCAATGGAGATGGCGTGCAGCCGAGTGGGGAGGAGCGTGTGGTGGGACTTGTACAATCCGACGGGGGCTGTCAATTCGCTGCTTGCGGATTCTGCGTGGTCTGATCAGCCCTTCTCCAGCTTCTGCCATCCATCCACCGTTCAGGACTTGGTATGGCCTTAAATCAATTCATTCATTTACGTGGGTTATTTGGTTGTTTCCGAAAACTCATTTGATCAATTGCAATTTCTCTCTTTGCAAGATAAATGAAACTTCAGTTCATACAAATGAAGGAAAAAAGGTTGCATATCAATCACTAAGTAGATATCTCTGTTTGTTTTTTTAGAAATTGGGAATAGAGAATATAAGAAAATGAATTATAACTCCTTATTGCTAAGTGGTTAAGTTGGGCTCTATATGTTGTCAACAAAAGAAAAGATTAATGGTAATAATTGTGTCCAGCTTCAGTGAAATAAAACTGGGTTATGCTACAAACTTATAGTTATACTAGTTACTTTGTTACTTGTAAGCTGTCATATTTTTAAATATAACATAAATAAATC

Coding sequence (CDS)

ATGGGCGTGGAGGAGCAGGTGTTGATTGGTGTGTTATTGGGGATGGTGGCAGTTGCAGTTGGAGTTGCAGATCCACCTCAAGTATCCAATGTCACCGCCATGTACTTGTTGGGAGATTCTTCTGTCGATTGCGGCTTAAGCACTCTCTATTATCCTCTTCTCCACCGCAATTTCTCTCTGTTTCCTTGTGAAGCCGACGCCGACGCCGACGCCTCTACTCTTCTCCCTCACTTTCTTGCTAAGAAGATAGGCGTGCCATACTCTCAACCCCTGTACACTCAGAACGGATCCATTGAAGCGATCCTAAATGGCCTCAACTTCGGTTCGCCACAAGCAACGATTATGAGCAGCAGCCGTAGCTATCAGTCCCTCAACCAACAGCTTCGTCAGGCACTCGACGCCATTCAGATGTTGCGCCTGCATCTCGGCCAAGACACTGCTCACCGTTTCATCCAATCATCCGTCTTCTACCTCTCCTTCGGCGAGATCGATTTCATTAATCTCTACCTCCTAAACTCGACAGGAAAGCAATTGAAGTACAAGGGCAAGGAATTTGGTCGGCTGCTGGTATCCCAGATGGTGATCGCCATAAGAAACCTGGAGGAAGCAGGCGCGAGGAAGATCATATGCATGGGAATACTGCCATTGGGGTGCACTCCTCGAGTGCTGTCTCTGTGGCGCAACTCCCCCGGGGCTGTATACGACGAGAAGGGATGTGTAAAAGAGATGAACGAGTTGGTTGCAGAGTACAACAGAGGGATGGAGAAGCAGATAGTGAAGTTCGGCAAAGGTCGGGTGGTATTCTGCGACGCGTACAAAGGGATGATGGAGATCGTGGGGAGCCCAAGGCGATACGGATTTGAGGAGTCGAAGAGTGCGTGTTGTGGGCTGGGTTGGTACAACGCGAGCATAGGATGCGTGGCAATGGAGATGGCGTGCAGCCGAGTGGGGAGGAGCGTGTGGTGGGACTTGTACAATCCGACGGGGGCTGTCAATTCGCTGCTTGCGGATTCTGCGTGGTCTGATCAGCCCTTCTCCAGCTTCTGCCATCCATCCACCGTTCAGGACTTGGTATGGCCTTAA

Protein sequence

MGVEEQVLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVKFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLVWP

Homology

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BLAST of MC02g0023 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 7.1e-33
Identity = 104/364 (28.57%), Postives = 173/364 (47.53%), Query Frame = 0


Query: 11  VLLGMVAVAVGVADPPQV---SNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADA 70
           VL+  + V+V +  P  V   + V AM++LGDS VD G +     +   NF  +  + + 
Sbjct: 16  VLILALTVSVILQQPELVTGQARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNY 75

Query: 71  D-----ADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMS-SSRSY 130
                 ++  T +   LA+ + +P   P      S   IL G+N+ S  A I+  S  +Y
Sbjct: 76  QPTGRFSNGLTFI-DLLARLLEIPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNY 135

Query: 131 Q---SLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLL-NSTGKQ 190
               SLNQQ+      +  LR  +       ++  S+  L FG  D+IN YL+ N     
Sbjct: 136 GGRFSLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSS 195

Query: 191 LKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEK 250
           ++++  +F  LL+SQ    +  L   G RKI   G+ PLGC P   +   + P       
Sbjct: 196 IRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDR----- 255

Query: 251 GCVKEMNELVAEYNRGMEK---QIVKFGKGRV-VFCDAYKGMMEIVGSPRRYGFEESKSA 310
            CV  +N+++  +N+G++    Q+ +   G + V+ + Y  + +I+ +P  YGF     A
Sbjct: 256 -CVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRA 315

Query: 311 CCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCHPST 358
           CCG+G     I C+ ++  C    + V+WD ++PT   NS+LA  A+   P  S  +P  
Sbjct: 316 CCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPP--SDAYPVN 370

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BLAST of MC02g0023 vs. ExPASy Swiss-Prot
Match: Q8L5Z1 (GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.9e-30
Identity = 96/367 (26.16%), Postives = 167/367 (45.50%), Query Frame = 0


Query: 7   VLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSL----FP 66
           +LI + L +      V+ P Q + V  +++ GDS VD G +     L   N+      FP
Sbjct: 8   LLISLNLVLFGFKTTVSQPQQQAQVPCLFIFGDSLVDNGNNNRLLSLARANYRPYGIDFP 67

Query: 67  CEADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSS---- 126
                           LA+ +G     P Y++    +AIL G NF S  A I   +    
Sbjct: 68  QGTTGRFTNGRTYVDALAQILGFRNYIPPYSRIRG-QAILRGANFASGAAGIRDETGDNL 127

Query: 127 RSYQSLNQQLRQALDAIQMLRLHLGQDT--AHRFIQSSVFYLSFGEIDFINLYLL-NSTG 186
            ++ S+NQQ+     A+Q +  +   DT    R++   +FY   G  D++N Y + +   
Sbjct: 128 GAHTSMNQQVELYTTAVQQMLRYFRGDTNELQRYLSRCIFYSGMGSNDYLNNYFMPDFYS 187

Query: 187 KQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYD 246
               Y  K F   L+      +  L + GARK+I  G+  +GC P  L+ + N   +   
Sbjct: 188 TSTNYNDKTFAESLIKNYTQQLTRLYQFGARKVIVTGVGQIGCIPYQLARYNNRNNST-- 247

Query: 247 EKGCVKEMNELVAEYNRGMEKQIVKFGKGRV-----VFCDAYKGMMEIVGSPRRYGFEES 306
              C +++N  +  +N  ++K + +  KG++     V+ D+YK   ++  +   YGFE  
Sbjct: 248 -GRCNEKINNAIVVFNTQVKKLVDRLNKGQLKGAKFVYLDSYKSTYDLAVNGAAYGFEVV 307

Query: 307 KSACCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCH 358
              CCG+G  N  I C+ ++  C    + ++WD ++PT   N LLA S +  +   ++ +
Sbjct: 308 DKGCCGVGRNNGQITCLPLQTPCPDRTKYLFWDAFHPTETANILLAKSNFYSR---AYTY 367

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BLAST of MC02g0023 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.0e-28
Identity = 89/364 (24.45%), Postives = 170/364 (46.70%), Query Frame = 0


Query: 7   VLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLST-LYYPLLHRNFSLFPCE- 66
           +++ + L +V      A P   S++   Y+ GDS  + G +  L Y L   +F  +  + 
Sbjct: 2   MILRLALAIVISTYATAQPASTSSLVT-YIFGDSLTEVGNNNFLQYSLARADFPYYGVDF 61

Query: 67  ----ADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRS 126
               A         +   ++ K+G+    P  + + + +A L+G+N+ S  A I++ +  
Sbjct: 62  SGGKATGRFTNGRTIGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGI 121

Query: 127 Y----QSLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGK 186
           Y     + N Q+       +++R  +G   A++ +  +++++  G  D++N +L      
Sbjct: 122 YFIQRLTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMAD 181

Query: 187 QLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTP--RVLSLWRNSPGAVY 246
             +Y   EF  LL S +   +  + + GARK+I  G+ PLGC P  RV S  R       
Sbjct: 182 GQQYTHDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTRM------ 241

Query: 247 DEKGCVKEMNELVAEYNRGMEKQIVKFGK----GRVVFCDAYKGMMEIVGSPRRYGFEES 306
               C+  +NE V E+N   +K ++   K     +  F D Y  +++++ +P  YGF+ +
Sbjct: 242 ----CLNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIA 301

Query: 307 KSACCGLGWYNASIG--CVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSSF 353
            ++CC +   + S+G  C+     C      V+WD ++P+ + N +LAD  +S    SS 
Sbjct: 302 NTSCCNV---DTSVGGLCLPNSKMCKNRQDFVFWDAFHPSDSANQILADHLFSSLLSSSS 351

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BLAST of MC02g0023 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 8.4e-26
Identity = 89/341 (26.10%), Postives = 158/341 (46.33%), Query Frame = 0


Query: 33  AMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTL-----LPHFLAKKIGVPY 92
           A ++ GDS VD G +  Y P L R  + FP   D             +  + A  +G+P 
Sbjct: 30  AFFVFGDSLVDSG-NNNYIPTLAR-ANYFPYGIDFGFPTGRFCNGRTVVDYGATYLGLPL 89

Query: 93  SQPLYTQNGSIEAILNGLNFGSPQATIM-SSSRSY---QSLNQQLRQALDAIQMLRLHLG 152
             P  +     +  L G+N+ S  A I+  + R Y    + N Q+ Q    I++      
Sbjct: 90  VPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFF 149

Query: 153 QDTA--HRFIQSSVFYLSFGEIDFINLYLL-NSTGKQLKYKGKEFGRLLVSQMVIAIRNL 212
           Q+ A   +++  S+  ++ G  D+IN YL+         Y G+++  LL+  +   I  L
Sbjct: 150 QNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRL 209

Query: 213 EEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQI-- 272
              GARK++  G  PLGC P  LS+   +     +  GCV ++N +V+ +N  ++     
Sbjct: 210 YNLGARKMVLAGSGPLGCIPSQLSMVTGN-----NTSGCVTKINNMVSMFNSRLKDLANT 269

Query: 273 --VKFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSRV 332
                     V+ + +    ++V +P RYG   S  ACCG G Y  ++ C+ ++  C   
Sbjct: 270 LNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDR 329

Query: 333 GRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDL 358
            + V+WD ++PT   N ++A + +S    +++ +P +V +L
Sbjct: 330 NQYVFWDAFHPTETANKIIAHNTFSKS--ANYSYPISVYEL 361

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BLAST of MC02g0023 vs. ExPASy Swiss-Prot
Match: Q9SIZ6 (GDSL esterase/lipase At2g40250 OS=Arabidopsis thaliana OX=3702 GN=At2g40250 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 9.3e-25
Identity = 89/349 (25.50%), Postives = 166/349 (47.56%), Query Frame = 0


Query: 8   LIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNF----SLFPC 67
           LI +LL  +      +  P    +TA+Y  GDS+VD G +     L   N       FP 
Sbjct: 14  LINILLLQLLNLTNASPSPP---ITALYAFGDSTVDSGNNNYIPTLFQSNHPPYGKSFPS 73

Query: 68  EADADA-DASTLLPHFLAKKIGVPYSQPLYTQNGSIEAI--LNGLNFGSPQATI---MSS 127
           +          L   F+   +G+  + P Y  N S++ +  L G++F S    +    + 
Sbjct: 74  KLSTGRFSDGKLATDFIVSSLGLKPTLPAYL-NPSVKPVDLLTGVSFASAGGGLDDRTAK 133

Query: 128 SRSYQSLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFI-NLY--LLNST 187
           S    ++++Q     +A+  ++  +G    +R I+++VF +S G  D I N+Y  +L S 
Sbjct: 134 SSLTITMDKQWSYFEEALGKMKSLVGDSETNRVIKNAVFVISAGTNDMIFNVYDHVLGSL 193

Query: 188 GKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVY 247
                Y+       L++++ + ++ L EAGAR+I   G+ P+GC P  ++L   +   ++
Sbjct: 194 ISVSDYQDS-----LLTKVEVFVQRLYEAGARRITIAGLPPIGCLPVQVTLTSINTPRIF 253

Query: 248 DEKGCVKEMNELVAEYNRGMEKQIV----KFGKGRVVFCDAYKGMMEIVGSPRRYGFEES 307
             + C +  N+    YN+ ++K I     +F   +V++ D Y  +++++  PR+YG EE+
Sbjct: 254 HHRICTEHQNDDSRVYNQKLQKLIFGLSQRFRGSKVLYLDIYSPLIDMIKHPRKYGLEET 313

Query: 308 KSACCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSA 340
              CCG G   A   C  +   C  V + +++D  +P+    S++A  A
Sbjct: 314 LRGCCGTGLLEAGPLCQPLSRTCDDVSKYLFFDSVHPSQTAYSVIASFA 353

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BLAST of MC02g0023 vs. NCBI nr
Match: XP_004149661.1 (GDSL esterase/lipase At1g71250 [Cucumis sativus])

HSP 1 Score: 497 bits (1279), Expect = 1.28e-173
Identity = 243/338 (71.89%), Postives = 286/338 (84.62%), Query Frame = 0


Query: 27  QVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVP 86
           Q SNV AM+LLGDSSVDCG +TL+YPLLHRNFSL PC+A+A    S+LLP  LA KIG+P
Sbjct: 25  QESNVNAMFLLGDSSVDCGFNTLFYPLLHRNFSLLPCDANA---TSSLLPFLLADKIGLP 84

Query: 87  YSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDT 146
           ++QP Y QN S+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  
Sbjct: 85  HAQPFYNQNESLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLLRLQLGQHA 144

Query: 147 AHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGAR 206
           A  FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGAR
Sbjct: 145 ARHFIQSSLFYLSFGEVDFINLYLLKSSER--KYGGEEFARLLVSQMVIAIRNLQEAGAR 204

Query: 207 KIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FG 266
           KI+CMGILPLGC+PRVLS WR+SP    D+KGCVKEMNELV +YN  ME+++VK    FG
Sbjct: 205 KIVCMGILPLGCSPRVLSEWRDSPADTLDKKGCVKEMNELVGKYNEVMEEEMVKLNAEFG 264

Query: 267 KGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVW 326
             +++FCD YKGMMEI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VW
Sbjct: 265 DTQMIFCDVYKGMMEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVW 324

Query: 327 WDLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLVW 359
           WDLYNPTG VN+LLADSAW ++PFS+ C PST+QDL++
Sbjct: 325 WDLYNPTGVVNALLADSAWGNRPFSTICRPSTIQDLLF 357

[Close]

BLAST of MC02g0023 vs. NCBI nr
Match: XP_008449874.1 (PREDICTED: GDSL esterase/lipase At1g71250-like isoform X2 [Cucumis melo] >KAA0040096.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 494 bits (1272), Expect = 1.20e-172
Identity = 242/335 (72.24%), Postives = 283/335 (84.48%), Query Frame = 0


Query: 29  SNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG +TL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNGQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 358
           LYNPTG VNSLLADSAW +QPFS+ CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLADSAWRNQPFSTICHPSTVQDLL 350

[Close]

BLAST of MC02g0023 vs. NCBI nr
Match: TYK21721.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 493 bits (1268), Expect = 4.87e-172
Identity = 241/335 (71.94%), Postives = 283/335 (84.48%), Query Frame = 0


Query: 29  SNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG +TL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 358
           LYNPTG VNSLLA+SAW +QPFS+ CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLAESAWRNQPFSTICHPSTVQDLL 350

[Close]

BLAST of MC02g0023 vs. NCBI nr
Match: XP_022958403.1 (GDSL esterase/lipase At1g71250-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 491 bits (1264), Expect = 8.34e-172
Identity = 245/336 (72.92%), Postives = 279/336 (83.04%), Query Frame = 0


Query: 27  QVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVP 86
           Q S++TAMYLLGDSSVDCGL+ L+YPLLHRNFS+ PC    D+D +TLLPH LA+++GVP
Sbjct: 3   QGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPC----DSDGTTLLPHLLAEEMGVP 62

Query: 87  YSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDT 146
           +SQP Y QNGSIEAILNGL+FGSPQATI+S  +S+QSLNQQLRQ LD +Q+LRL LG DT
Sbjct: 63  HSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDT 122

Query: 147 AHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGAR 206
           A  FIQSS+FY SFGE+DFI   L +S GK       +F +LL SQM +AIRNL EAGA+
Sbjct: 123 ARHFIQSSLFYFSFGEVDFI---LDSSRGKN----NLKFPQLLASQMSVAIRNLHEAGAK 182

Query: 207 KIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIV----KFG 266
           KI+CMGILPLGCTPRVLS WR+SP AVYDEKGCVKEMNELV  YN  MEKQIV    + G
Sbjct: 183 KIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLG 242

Query: 267 KGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSRVGRSVWW 326
             R+VFCDAYKGMMEI+G+PRRYGFEES+SACCGLG YNASIGCVA E+AC RV R VWW
Sbjct: 243 DARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWW 302

Query: 327 DLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 358
           DLYNPTGAVNSLLA+SAW DQPFSSFC PST+ DLV
Sbjct: 303 DLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLV 327

[Close]

BLAST of MC02g0023 vs. NCBI nr
Match: KAG7035797.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491 bits (1265), Expect = 1.67e-171
Identity = 246/342 (71.93%), Postives = 282/342 (82.46%), Query Frame = 0


Query: 21  GVADPPQVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLA 80
           G++   Q S++TAMYLLGDSSVDCGL+ L+YPLLHRNFS+ PC    D+D +TLLPH LA
Sbjct: 26  GLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPC----DSDGTTLLPHLLA 85

Query: 81  KKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRL 140
           +++GVP+SQP Y QNGSIEAILNGL+FGSPQATI+S  +S+QSLNQQLRQ LD +Q+LRL
Sbjct: 86  EEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRL 145

Query: 141 HLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNL 200
            LG DTA  FIQSS+FY SFGE+DFI   L +S GK       +F +LL SQM +AIRNL
Sbjct: 146 QLGLDTARHFIQSSLFYFSFGEVDFI---LDSSRGKN----NLKFPQLLASQMSVAIRNL 205

Query: 201 EEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIV- 260
            EAGA+KI+CMGILPLGCTPRVLS WR+SP AVYDEKGCVKEMNELV  YN  MEKQIV 
Sbjct: 206 HEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVM 265

Query: 261 ---KFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSRV 320
              + G  R+VFCDAYKGMMEI+G+PRRYGFEES+SACCGLG YNASIGCVA E+AC RV
Sbjct: 266 LNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRV 325

Query: 321 GRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 358
            R VWWDLYNPTGAVNSLLA+SAW DQPFSSFC PST+ DLV
Sbjct: 326 RRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLV 356

[Close]

BLAST of MC02g0023 vs. ExPASy TrEMBL
Match: A0A0A0L1P2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G269190 PE=3 SV=1)

HSP 1 Score: 497 bits (1279), Expect = 6.21e-174
Identity = 243/338 (71.89%), Postives = 286/338 (84.62%), Query Frame = 0


Query: 27  QVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVP 86
           Q SNV AM+LLGDSSVDCG +TL+YPLLHRNFSL PC+A+A    S+LLP  LA KIG+P
Sbjct: 25  QESNVNAMFLLGDSSVDCGFNTLFYPLLHRNFSLLPCDANA---TSSLLPFLLADKIGLP 84

Query: 87  YSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDT 146
           ++QP Y QN S+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  
Sbjct: 85  HAQPFYNQNESLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLLRLQLGQHA 144

Query: 147 AHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGAR 206
           A  FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGAR
Sbjct: 145 ARHFIQSSLFYLSFGEVDFINLYLLKSSER--KYGGEEFARLLVSQMVIAIRNLQEAGAR 204

Query: 207 KIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FG 266
           KI+CMGILPLGC+PRVLS WR+SP    D+KGCVKEMNELV +YN  ME+++VK    FG
Sbjct: 205 KIVCMGILPLGCSPRVLSEWRDSPADTLDKKGCVKEMNELVGKYNEVMEEEMVKLNAEFG 264

Query: 267 KGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVW 326
             +++FCD YKGMMEI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VW
Sbjct: 265 DTQMIFCDVYKGMMEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVW 324

Query: 327 WDLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLVW 359
           WDLYNPTG VN+LLADSAW ++PFS+ C PST+QDL++
Sbjct: 325 WDLYNPTGVVNALLADSAWGNRPFSTICRPSTIQDLLF 357

[Close]

BLAST of MC02g0023 vs. ExPASy TrEMBL
Match: A0A5A7TDD0 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00690 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 5.81e-173
Identity = 242/335 (72.24%), Postives = 283/335 (84.48%), Query Frame = 0


Query: 29  SNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG +TL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNGQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 358
           LYNPTG VNSLLADSAW +QPFS+ CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLADSAWRNQPFSTICHPSTVQDLL 350

[Close]

BLAST of MC02g0023 vs. ExPASy TrEMBL
Match: A0A1S3BNP3 (GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491620 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 5.81e-173
Identity = 242/335 (72.24%), Postives = 283/335 (84.48%), Query Frame = 0


Query: 29  SNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG +TL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNGQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 358
           LYNPTG VNSLLADSAW +QPFS+ CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLADSAWRNQPFSTICHPSTVQDLL 350

[Close]

BLAST of MC02g0023 vs. ExPASy TrEMBL
Match: A0A5D3DDH5 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00910 PE=3 SV=1)

HSP 1 Score: 493 bits (1268), Expect = 2.36e-172
Identity = 241/335 (71.94%), Postives = 283/335 (84.48%), Query Frame = 0


Query: 29  SNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG +TL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 358
           LYNPTG VNSLLA+SAW +QPFS+ CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLAESAWRNQPFSTICHPSTVQDLL 350

[Close]

BLAST of MC02g0023 vs. ExPASy TrEMBL
Match: A0A6J1H303 (GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459637 PE=3 SV=1)

HSP 1 Score: 491 bits (1264), Expect = 4.04e-172
Identity = 245/336 (72.92%), Postives = 279/336 (83.04%), Query Frame = 0


Query: 27  QVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVP 86
           Q S++TAMYLLGDSSVDCGL+ L+YPLLHRNFS+ PC    D+D +TLLPH LA+++GVP
Sbjct: 3   QGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPC----DSDGTTLLPHLLAEEMGVP 62

Query: 87  YSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDT 146
           +SQP Y QNGSIEAILNGL+FGSPQATI+S  +S+QSLNQQLRQ LD +Q+LRL LG DT
Sbjct: 63  HSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDT 122

Query: 147 AHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGAR 206
           A  FIQSS+FY SFGE+DFI   L +S GK       +F +LL SQM +AIRNL EAGA+
Sbjct: 123 ARHFIQSSLFYFSFGEVDFI---LDSSRGKN----NLKFPQLLASQMSVAIRNLHEAGAK 182

Query: 207 KIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIV----KFG 266
           KI+CMGILPLGCTPRVLS WR+SP AVYDEKGCVKEMNELV  YN  MEKQIV    + G
Sbjct: 183 KIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLG 242

Query: 267 KGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSRVGRSVWW 326
             R+VFCDAYKGMMEI+G+PRRYGFEES+SACCGLG YNASIGCVA E+AC RV R VWW
Sbjct: 243 DARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWW 302

Query: 327 DLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 358
           DLYNPTGAVNSLLA+SAW DQPFSSFC PST+ DLV
Sbjct: 303 DLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLV 327

[Close]

BLAST of MC02g0023 vs. TAIR 10
Match: AT4G10950.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 188.7 bits (478), Expect = 8.0e-48
Identity = 104/344 (30.23%), Postives = 176/344 (51.16%), Query Frame = 0


Query: 26  PQVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADAST---LLPHFLAKK 85
           P +  V A+++ GDSSVD G +     L   +    P   D D    T            
Sbjct: 64  PSLPFVPALFVFGDSSVDSGTNNFLGTLARAD--RLPYGRDFDTHQPTGRFCNGRIPVDY 123

Query: 86  IGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRS----YQSLNQQLRQALDAIQML 145
           +G+P+      Q G++E +  G+N+ S  A I+ SS S      S   Q+ Q +D  Q +
Sbjct: 124 LGLPFVPSYLGQTGTVEDMFQGVNYASAGAGIILSSGSELGQRVSFAMQVEQFVDTFQQM 183

Query: 146 RLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIR 205
            L +G+  + R + +SVFY+S G  D+I+ Y+ N +  Q  Y    F + L S M   ++
Sbjct: 184 ILSIGEKASERLVSNSVFYISIGVNDYIHFYIRNISNVQNLYTPWNFNQFLASNMRQELK 243

Query: 206 NLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQI 265
            L     R+++ MG+ P+GC P  +  +R+  G       C +E+N ++ E N  M   +
Sbjct: 244 TLYNVKVRRMVVMGLPPIGCAPYYMWKYRSQNGE------CAEEVNSMIMESNFVMRYTV 303

Query: 266 VKFGK----GRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACS 325
            K  +      +++CD ++  M+I+ + + YGF E+  ACCGLG Y   + C++ EMACS
Sbjct: 304 DKLNRELPGASIIYCDVFQSAMDILRNHQHYGFNETTDACCGLGRYKGWLPCISPEMACS 363

Query: 326 RVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDLV 359
                +WWD ++PT AVN++LAD+ W+ +     C+P+ ++ ++
Sbjct: 364 DASGHLWWDQFHPTDAVNAILADNVWNGR-HVDMCYPTNLETML 398

[Close]

BLAST of MC02g0023 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 142.9 bits (359), Expect = 5.0e-34
Identity = 104/364 (28.57%), Postives = 173/364 (47.53%), Query Frame = 0


Query: 11  VLLGMVAVAVGVADPPQV---SNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADA 70
           VL+  + V+V +  P  V   + V AM++LGDS VD G +     +   NF  +  + + 
Sbjct: 16  VLILALTVSVILQQPELVTGQARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNY 75

Query: 71  D-----ADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMS-SSRSY 130
                 ++  T +   LA+ + +P   P      S   IL G+N+ S  A I+  S  +Y
Sbjct: 76  QPTGRFSNGLTFI-DLLARLLEIPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNY 135

Query: 131 Q---SLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLL-NSTGKQ 190
               SLNQQ+      +  LR  +       ++  S+  L FG  D+IN YL+ N     
Sbjct: 136 GGRFSLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSS 195

Query: 191 LKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEK 250
           ++++  +F  LL+SQ    +  L   G RKI   G+ PLGC P   +   + P       
Sbjct: 196 IRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDR----- 255

Query: 251 GCVKEMNELVAEYNRGMEK---QIVKFGKGRV-VFCDAYKGMMEIVGSPRRYGFEESKSA 310
            CV  +N+++  +N+G++    Q+ +   G + V+ + Y  + +I+ +P  YGF     A
Sbjct: 256 -CVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRA 315

Query: 311 CCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCHPST 358
           CCG+G     I C+ ++  C    + V+WD ++PT   NS+LA  A+   P  S  +P  
Sbjct: 316 CCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPP--SDAYPVN 370

[Close]

BLAST of MC02g0023 vs. TAIR 10
Match: AT1G33811.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.4e-31
Identity = 96/367 (26.16%), Postives = 167/367 (45.50%), Query Frame = 0


Query: 7   VLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLSTLYYPLLHRNFSL----FP 66
           +LI + L +      V+ P Q + V  +++ GDS VD G +     L   N+      FP
Sbjct: 8   LLISLNLVLFGFKTTVSQPQQQAQVPCLFIFGDSLVDNGNNNRLLSLARANYRPYGIDFP 67

Query: 67  CEADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSS---- 126
                           LA+ +G     P Y++    +AIL G NF S  A I   +    
Sbjct: 68  QGTTGRFTNGRTYVDALAQILGFRNYIPPYSRIRG-QAILRGANFASGAAGIRDETGDNL 127

Query: 127 RSYQSLNQQLRQALDAIQMLRLHLGQDT--AHRFIQSSVFYLSFGEIDFINLYLL-NSTG 186
            ++ S+NQQ+     A+Q +  +   DT    R++   +FY   G  D++N Y + +   
Sbjct: 128 GAHTSMNQQVELYTTAVQQMLRYFRGDTNELQRYLSRCIFYSGMGSNDYLNNYFMPDFYS 187

Query: 187 KQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYD 246
               Y  K F   L+      +  L + GARK+I  G+  +GC P  L+ + N   +   
Sbjct: 188 TSTNYNDKTFAESLIKNYTQQLTRLYQFGARKVIVTGVGQIGCIPYQLARYNNRNNST-- 247

Query: 247 EKGCVKEMNELVAEYNRGMEKQIVKFGKGRV-----VFCDAYKGMMEIVGSPRRYGFEES 306
              C +++N  +  +N  ++K + +  KG++     V+ D+YK   ++  +   YGFE  
Sbjct: 248 -GRCNEKINNAIVVFNTQVKKLVDRLNKGQLKGAKFVYLDSYKSTYDLAVNGAAYGFEVV 307

Query: 307 KSACCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCH 358
              CCG+G  N  I C+ ++  C    + ++WD ++PT   N LLA S +  +   ++ +
Sbjct: 308 DKGCCGVGRNNGQITCLPLQTPCPDRTKYLFWDAFHPTETANILLAKSNFYSR---AYTY 367

[Close]

BLAST of MC02g0023 vs. TAIR 10
Match: AT5G37690.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 127.1 bits (318), Expect = 2.9e-29
Identity = 89/364 (24.45%), Postives = 170/364 (46.70%), Query Frame = 0


Query: 7   VLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLST-LYYPLLHRNFSLFPCE- 66
           +++ + L +V      A P   S++   Y+ GDS  + G +  L Y L   +F  +  + 
Sbjct: 2   MILRLALAIVISTYATAQPASTSSLVT-YIFGDSLTEVGNNNFLQYSLARADFPYYGVDF 61

Query: 67  ----ADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRS 126
               A         +   ++ K+G+    P  + + + +A L+G+N+ S  A I++ +  
Sbjct: 62  SGGKATGRFTNGRTIGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGI 121

Query: 127 Y----QSLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGK 186
           Y     + N Q+       +++R  +G   A++ +  +++++  G  D++N +L      
Sbjct: 122 YFIQRLTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMAD 181

Query: 187 QLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTP--RVLSLWRNSPGAVY 246
             +Y   EF  LL S +   +  + + GARK+I  G+ PLGC P  RV S  R       
Sbjct: 182 GQQYTHDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTRM------ 241

Query: 247 DEKGCVKEMNELVAEYNRGMEKQIVKFGK----GRVVFCDAYKGMMEIVGSPRRYGFEES 306
               C+  +NE V E+N   +K ++   K     +  F D Y  +++++ +P  YGF+ +
Sbjct: 242 ----CLNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIA 301

Query: 307 KSACCGLGWYNASIG--CVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSSF 353
            ++CC +   + S+G  C+     C      V+WD ++P+ + N +LAD  +S    SS 
Sbjct: 302 NTSCCNV---DTSVGGLCLPNSKMCKNRQDFVFWDAFHPSDSANQILADHLFSSLLSSSS 351

[Close]

BLAST of MC02g0023 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 119.4 bits (298), Expect = 6.0e-27
Identity = 89/341 (26.10%), Postives = 158/341 (46.33%), Query Frame = 0


Query: 33  AMYLLGDSSVDCGLSTLYYPLLHRNFSLFPCEADADADASTL-----LPHFLAKKIGVPY 92
           A ++ GDS VD G +  Y P L R  + FP   D             +  + A  +G+P 
Sbjct: 30  AFFVFGDSLVDSG-NNNYIPTLAR-ANYFPYGIDFGFPTGRFCNGRTVVDYGATYLGLPL 89

Query: 93  SQPLYTQNGSIEAILNGLNFGSPQATIM-SSSRSY---QSLNQQLRQALDAIQMLRLHLG 152
             P  +     +  L G+N+ S  A I+  + R Y    + N Q+ Q    I++      
Sbjct: 90  VPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFF 149

Query: 153 QDTA--HRFIQSSVFYLSFGEIDFINLYLL-NSTGKQLKYKGKEFGRLLVSQMVIAIRNL 212
           Q+ A   +++  S+  ++ G  D+IN YL+         Y G+++  LL+  +   I  L
Sbjct: 150 QNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRL 209

Query: 213 EEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQI-- 272
              GARK++  G  PLGC P  LS+   +     +  GCV ++N +V+ +N  ++     
Sbjct: 210 YNLGARKMVLAGSGPLGCIPSQLSMVTGN-----NTSGCVTKINNMVSMFNSRLKDLANT 269

Query: 273 --VKFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSRV 332
                     V+ + +    ++V +P RYG   S  ACCG G Y  ++ C+ ++  C   
Sbjct: 270 LNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDR 329

Query: 333 GRSVWWDLYNPTGAVNSLLADSAWSDQPFSSFCHPSTVQDL 358
            + V+WD ++PT   N ++A + +S    +++ +P +V +L
Sbjct: 330 NQYVFWDAFHPTETANKIIAHNTFSKS--ANYSYPISVYEL 361

The following BLAST results are available for this feature:

BLAST of MC02g0023 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FVV1	7.1e-33	28.57	GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2...	[more]
Q8L5Z1	1.9e-30	26.16	GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2...	[more]
Q9FHQ1	4.0e-28	24.45	GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2...	[more]
Q8LFJ9	8.4e-26	26.10	GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1	[more]
Q9SIZ6	9.3e-25	25.50	GDSL esterase/lipase At2g40250 OS=Arabidopsis thaliana OX=3702 GN=At2g40250 PE=2...	[more]

BLAST of MC02g0023 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149661.1	1.28e-173	71.89	GDSL esterase/lipase At1g71250 [Cucumis sativus]	[more]
XP_008449874.1	1.20e-172	72.24	PREDICTED: GDSL esterase/lipase At1g71250-like isoform X2 [Cucumis melo] >KAA004...	[more]
TYK21721.1	4.87e-172	71.94	GDSL esterase/lipase [Cucumis melo var. makuwa]	[more]
XP_022958403.1	8.34e-172	72.92	GDSL esterase/lipase At1g71250-like isoform X2 [Cucurbita moschata]	[more]
KAG7035797.1	1.67e-171	71.93	GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC02g0023 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1P2	6.21e-174	71.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G269190 PE=3 SV=1	[more]
A0A5A7TDD0	5.81e-173	72.24	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366...	[more]
A0A1S3BNP3	5.81e-173	72.24	GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A5D3DDH5	2.36e-172	71.94	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859...	[more]
A0A6J1H303	4.04e-172	72.92	GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of MC02g0023 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10950.1	8.0e-48	30.23	SGNH hydrolase-type esterase superfamily protein	[more]
AT1G71250.1	5.0e-34	28.57	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G33811.1	1.4e-31	26.16	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G37690.1	2.9e-29	24.45	SGNH hydrolase-type esterase superfamily protein	[more]
AT5G15720.1	6.0e-27	26.10	GDSL-motif lipase 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 121..141
None	No IPR available	PANTHER	PTHR45648:SF7	OS12G0126100 PROTEIN	coord: 26..358
None	No IPR available	PANTHER	PTHR45648	GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)	coord: 26..358
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 36..273 e-value: 1.8E-7 score: 31.9
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 25..343 e-value: 5.0E-47 score: 162.9

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g0023.1	MC02g0023.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds