MC02g0007 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g0007 vs. ExPASy Swiss-Prot
Match: Q9STQ2 (Subtilisin-like protease SBT3.18 OS=Arabidopsis thaliana OX=3702 GN=SBT3.18 PE=3 SV=2)

HSP 1 Score: 825.1 bits (2130), Expect = 6.1e-238
Identity = 428/719 (59.53%), Postives = 537/719 (74.69%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSY + F GFSAKLN++QA  L+++  VI+VF+SK+L+LHTTRSWDFLGL +     R
Sbjct: 68  MLYSYNNGFLGFSAKLNSTQAASLAKLNQVITVFKSKSLKLHTTRSWDFLGLAV-DNARR 127

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
           TP PPQLAYG D+VVG+FD+G+WPESESF+E     P+P SW GKCV    FDP   CN 
Sbjct: 128 TP-PPQLAYGSDIVVGIFDTGIWPESESFRETPEAKPIPSSWNGKCVGGEDFDPSVHCNR 187

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGAR+YL+GFE  YG ++ +      E+ SPRD+LGHGTHTAST VGSVV +VS    
Sbjct: 188 KLIGARFYLRGFEETYGTIDFT---RDPEYRSPRDYLGHGTHTASTAVGSVVRNVSGF-F 247

Query: 181 GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP 240
           GLGRG ARGGAP ARLAV+K CWGKD +G CTEAD++AAFDDA+ DGVHVISAS G +PP
Sbjct: 248 GLGRGTARGGAPLARLAVFKTCWGKDLEGVCTEADILAAFDDAIHDGVHVISASFGYSPP 307

Query: 241 LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI 300
           L PF +SSA IG+FHA ++G+SVVFS GNDGP P +VQNV+PW++ VAAST DR+FPT+I
Sbjct: 308 LSPFFESSADIGAFHAAERGISVVFSTGNDGPDPGVVQNVAPWAVSVAASTVDRSFPTRI 367

Query: 301 QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST 360
            I   +   ++TG SLI++ IT   LA A  YF  G+C+  +  K  K     ++LCFST
Sbjct: 368 VI---DGSFTLTGQSLISQEITGT-LALATTYFNGGVCKWENWMK--KLANETIILCFST 427

Query: 361 IGPTS-IEVAQEAVMTINASALIFAAPPTTQL-PDLDLIPTVRLDITRATQIRNVLTELP 420
           +GP   IE AQ A +  NA ALIFAA PT QL  ++D+IPTVR+DI   T+IRN L   P
Sbjct: 428 LGPVQFIEEAQAAAIRANALALIFAASPTRQLAEEVDMIPTVRVDILHGTRIRNYLARSP 487

Query: 421 RLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPT 480
            +P V+IG +K VIG++ APSVAYFSSRGPSSLSP+ILKPDI+APG+ ILAAWPP T PT
Sbjct: 488 TVPMVKIGPSKTVIGETTAPSVAYFSSRGPSSLSPDILKPDITAPGIGILAAWPPRTPPT 547

Query: 481 VRPGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTG 540
           + PG   S  ++WNFQSGTSMSCPHV+GV+AL++SAHP WSP+AIRSA++TTA  RD++ 
Sbjct: 548 LLPGDHRS--IEWNFQSGTSMSCPHVAGVMALLQSAHPDWSPSAIRSAIMTTAYTRDTSY 607

Query: 541 DTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIR-MLL 600
           D IL+GGSMK++DPFD+GAG +NPLKA+DPGLVY+   +DY+ F+C++GYT+ +I+ M+L
Sbjct: 608 DLILSGGSMKSTDPFDIGAGHINPLKAMDPGLVYNTRTDDYVLFMCNIGYTDQEIKSMVL 667

Query: 601 NPSPDQAHTPLCCPSNLV---ANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVL 660
           +P P    +  C PS+     A+ NYPSIT+  L  T TIKRT+ NV PNKN VYF+ ++
Sbjct: 668 HPEP----STTCLPSHSYRTNADFNYPSITIPSLRLTRTIKRTVSNVGPNKNTVYFVDII 727

Query: 661 PPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVV 714
            P GV V++WPRIL FS   Q  SYYVT  P +    RY FGEI W+NG H V SP+VV
Sbjct: 728 RPVGVEVLIWPRILVFSKCQQEHSYYVTFKPTEIFSGRYVFGEIMWTNGLHRVRSPVVV 768

BLAST of MC02g0007 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.4e-144
Identity = 304/716 (42.46%), Postives = 424/716 (59.22%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           ++YSY+H FSGF+AKL  SQA ++S++  V+ V  +   ++ TTR+WD+LG+    P   
Sbjct: 67  IVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGV---SPGNS 126

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
             L  +   GY+V+VGV DSGVWPESE F +    GP+P  WKG C     F+    CN 
Sbjct: 127 DSLLQKANMGYNVIVGVIDSGVWPESEMFNDK-GFGPIPSRWKGGCESGELFNASIHCNR 186

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGA+Y++ G  A++G +N   + NP E+ SPRDF GHGTH AST+ GS + +VS++  
Sbjct: 187 KLIGAKYFVDGLVAEFGVVN--RTQNP-EYLSPRDFAGHGTHVASTIGGSFLPNVSYV-- 246

Query: 181 GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP 240
           GLGRG ARGGAP   +AVYK CW     G C+ AD++ A D+A+ DGV ++S SLG + P
Sbjct: 247 GLGRGTARGGAPGVHIAVYKACW----SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVP 306

Query: 241 LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI 300
           L P  + ++ +G+FHA+ +G+ VV + GN GP    + NV+PW + VAA+T DR+FPT I
Sbjct: 307 LFPETEHTS-VGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAI 366

Query: 301 QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST 360
            +      I+I G ++                   G CE+ S    S +  GKVVLCF+ 
Sbjct: 367 TL---GNNITILGQAIYGGPELGFVGLTYPESPLSGDCEKLSANPNS-TMEGKVVLCFAA 426

Query: 361 IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELPRL 420
             P++  +A  AV+      LI A  PT  L      P V +D    T I   +    R 
Sbjct: 427 STPSNAAIA--AVINAGGLGLIMAKNPTHSLTPTRKFPWVSIDFELGTDILFYIRS-TRS 486

Query: 421 PAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPTVR 480
           P V+I  +K + G+S +  VA FSSRGP+S+SP ILKPDI+APGVNILAA  P +  ++ 
Sbjct: 487 PIVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNS--SIN 546

Query: 481 PGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTGDT 540
            G        +   SGTSM+ P VSGVV L+KS HP WSP+AI+SA++TTA R D +G+ 
Sbjct: 547 DG-------GFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEP 606

Query: 541 ILA-GGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLLNP 600
           I A G S K +DPFD G G +NP KA+ PGL+YDMT +DY+ ++CS+ Y++  I  +L  
Sbjct: 607 IFADGSSRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGK 666

Query: 601 SPDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPSGV 660
                 T    P   V +LN PSIT+  L    T+ RT+ NV P  N+VY + + PP+G+
Sbjct: 667 I-----TVCPNPKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPPTGI 726

Query: 661 RVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT 716
            V V P  L F   T + S+ V ++   K    Y FG + W++  H+V  P+ V T
Sbjct: 727 NVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVRT 746

BLAST of MC02g0007 vs. ExPASy Swiss-Prot
Match: Q9ZSB1 (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 502.3 bits (1292), Expect = 9.1e-141
Identity = 303/718 (42.20%), Postives = 419/718 (58.36%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           ++YSY+H FSGF+AKL  SQA ++S++  V+ V  +   ++ TTR+WD+LG+    P   
Sbjct: 67  IVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGV---SPGNS 126

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
             L  +   GY+V+VGV D+GVWPESE F +    GP+P  WKG C     F+    CN 
Sbjct: 127 DSLLQKANMGYNVIVGVIDTGVWPESEMFNDK-GYGPIPSRWKGGCESGELFNGSIHCNR 186

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGA+Y++    A++G LN   + NP ++ SPRDF GHGTH AST+ GS + +VS+L  
Sbjct: 187 KLIGAKYFIDANNAQFGVLN--KTENP-DYLSPRDFNGHGTHVASTIGGSFLPNVSYL-- 246

Query: 181 GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP 240
           GLGRG ARGGAP   +AVYK CW    Q  C+ AD++ A D+A+ DGV ++S SL  + P
Sbjct: 247 GLGRGTARGGAPGVHIAVYKACW---VQRGCSGADVLKAMDEAIHDGVDILSLSLQTSVP 306

Query: 241 LDPFLDSS--AGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPT 300
           L P  D+     +G+FHA+ +G+ VV +  N GP    + NV+PW + VAA+T DR+FPT
Sbjct: 307 LFPETDARELTSVGAFHAVAKGIPVVAAASNAGPTAQTLSNVAPWVLTVAATTQDRSFPT 366

Query: 301 QIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCF 360
            I +      I+I G ++   S               G CE+ S    S +  GKVVLCF
Sbjct: 367 AITL---GNNITILGQAIFGGSELGFVGLTYPESPLSGDCEKLSANPKS-AMEGKVVLCF 426

Query: 361 STIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELP 420
           +   P++  +   AV+      LI A  PT  L  L   P V +D    T I   +    
Sbjct: 427 AASTPSNAAIT--AVINAGGLGLIMARNPTHLLRPLRNFPYVSVDFELGTDILFYIRS-T 486

Query: 421 RLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPT 480
           R P V I  ++ + G+S +  VA FSSRGP+S+SP ILKPDI+APGVNILAA  P    +
Sbjct: 487 RSPIVNIQASRTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPN---S 546

Query: 481 VRPGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTG 540
           +  G        +   SGTSM+ P VSGVV L+KS HP WSP+AI+SA++TTA R D +G
Sbjct: 547 INDG-------GFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSG 606

Query: 541 DTILA-GGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLL 600
           + I A G S K +DPFD G G +NP KA+ PGL+YDMT +DY+ ++CS+ Y++  I  +L
Sbjct: 607 EPIFADGSSRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVL 666

Query: 601 NPSPDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPS 660
                   T    P   V +LN PSIT+  L    T+ RT+ NV P  N+VY + + PP+
Sbjct: 667 GKI-----TVCPNPKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPPT 726

Query: 661 GVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT 716
           GV V V P  L F   T + S+ V ++   K    Y FG + W++  H+V  P+ V T
Sbjct: 727 GVNVAVTPTELVFDSTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDTLHNVAIPVSVRT 749

BLAST of MC02g0007 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 4.5e-140
Identity = 307/727 (42.23%), Postives = 421/727 (57.91%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           M+YSY+H FSGF+AKL  SQA +L+    V+ V      +L TTR+WD+LGL +  P   
Sbjct: 67  MVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANP--- 126

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
             L      G  V++G  D+GVWPESESF +   +GP+P  WKG C    +F   + CN 
Sbjct: 127 NNLLNDTNMGDQVIIGFIDTGVWPESESFNDN-GVGPIPSHWKGGCESGEKF-ISTNCNR 186

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGA+Y++ GF A+    N + S    ++ S RDF+GHGTHTAS   GS V ++S+   
Sbjct: 187 KLIGAKYFINGFLAENEGFNTTES---RDYISARDFIGHGTHTASIAGGSFVPNISY--K 246

Query: 181 GLGRGIARGGAPRARLAVYKVCWGKDYQG--KCTEADLMAAFDDALRDGVHVISASLGGT 240
           GL  G  RGGAPRAR+A+YK CW  D  G   C+ +D++ A D+++ DGV V+S SLG  
Sbjct: 247 GLAGGNLRGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQ 306

Query: 241 PPLDPFLD--SSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
            PL P  D       G+FHA+ +G+ VV +GGN GP    V N +PW I VAA+T DR+F
Sbjct: 307 IPLYPETDLRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSF 366

Query: 301 PTQIQILLPNRRISI-----TGDSLITESITNAKLADAINYFTDGICERASIRKASKSGA 360
           PT   I L NR++ +     TG  L   S+   + A   N    G+CER ++   +++ A
Sbjct: 367 PT--PITLGNRKVILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNL-NPNRTMA 426

Query: 361 GKVVLCFSTIGP-TSIEVAQEAVMTINASALIFAAPPTTQL-PDLDLIPTVRLDITRATQ 420
           GKVVLCF+T    T++  A   V       +I A  P   L P  D  P V +D    T 
Sbjct: 427 GKVVLCFTTNTLFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTD 486

Query: 421 IRNVLTELPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILA 480
           +  +     R P V+I  ++ ++G+     VA FSSRGP+S+SP ILKPDI APGV+ILA
Sbjct: 487 VL-LYIRSTRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILA 546

Query: 481 AWPPETAPTVRPGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALIT 540
           A  P++  +V           ++  +GTSM+ P V+GVVAL+K+ HP WSPAA RSA++T
Sbjct: 547 ATSPDSNSSVG---------GFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVT 606

Query: 541 TATRRDSTGDTILA-GGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGY 600
           TA R D  G+ I A G S K +DPFD G G VNP KA DPGL+YDM   DYI +LCS GY
Sbjct: 607 TAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGY 666

Query: 601 TEHQIRMLLNPSPDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAV 660
            +  I  L+        T    P   V ++N PSIT+  L    T+ RT+ NV    ++V
Sbjct: 667 NDSSITQLVGNV-----TVCSTPKTSVLDVNLPSITIPDLKDEVTLTRTVTNVG-TVDSV 726

Query: 661 YFLRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVT 716
           Y + V PP G++VVV P  L F+  T+ +S+ V ++   K    + FG + W++  H+VT
Sbjct: 727 YKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVT 764

BLAST of MC02g0007 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 9.8e-135
Identity = 303/728 (41.62%), Postives = 413/728 (56.73%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           M+YSY+H FSGF+AKL  SQA +++ +  V+ V      +L TTR+WD+LGL    P + 
Sbjct: 72  MVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS- 131

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
             L  +   G  +++GV D+GVWPESE F ++   GPVP  WKG C     F+  S CN 
Sbjct: 132 --LLHETNMGEQIIIGVIDTGVWPESEVFNDS-GFGPVPSHWKGGCETGENFN-SSNCNK 191

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGA+Y++ GF A+  + N   S N  +F SPRD  GHGTH ++   GS V ++S+   
Sbjct: 192 KLIGAKYFINGFLAENESFN---STNSLDFISPRDLDGHGTHVSTIAGGSFVPNISY--K 251

Query: 181 GLGRGIARGGAPRARLAVYKVCW--GKDYQGKCTEADLMAAFDDALRDGVHVISASLGGT 240
           GL  G  RGGAPRA +A+YK CW    D    C+ AD++ A D+A+ DGV V+S SLG +
Sbjct: 252 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSS 311

Query: 241 PPLDPFLDSSAGI--GSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
            PL    D   GI  G+FHA+ +G++VV SGGN GP    V N +PW I VAA+T DR+F
Sbjct: 312 VPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSF 371

Query: 301 PTQIQILLPNRRISI-----TGDSLITESITNAKLADAINYFTDGICERASIRKASKSGA 360
            T   + L N ++ +     TG  L   S+   +     N    G CE   +  ++++  
Sbjct: 372 AT--PLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEEL-LFNSNRTME 431

Query: 361 GKVVLCFST--IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQ 420
           GKVVLCF+T   G   +  A+          +I   P     P LD  P V +D    T 
Sbjct: 432 GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTD 491

Query: 421 IRNVLTELPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILA 480
           I  + T     P V+I  +K ++G+     VA FSSRGP+S++P ILKPDI+APGV+ILA
Sbjct: 492 IL-LYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILA 551

Query: 481 AWPPETAPTVRPGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALIT 540
           A       T        G +     SGTSM+ P +SGV AL+K+ H  WSPAAIRSA++T
Sbjct: 552 A-------TTNTTFSDQGFI---MLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVT 611

Query: 541 TATRRDSTGDTILAGGS-MKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGY 600
           TA + D  G+ I A GS  K +DPFD G G VNP K+ +PGLVYDM   DY+ ++CS+GY
Sbjct: 612 TAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGY 671

Query: 601 TEHQIRMLLNPSPDQAHTPLCC-PSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNA 660
            E  I  L+        T +C  P   V + N PSIT+  L    TI RT+ NV P  N+
Sbjct: 672 NETSISQLI------GKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGP-LNS 731

Query: 661 VYFLRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSV 716
           VY + V PP G +V V P  L F+  T+++ + V ++   K+   Y FG + WS+  H+V
Sbjct: 732 VYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNV 768

BLAST of MC02g0007 vs. NCBI nr
Match: XP_022153781.1 (subtilisin-like protease SBT3.18 [Momordica charantia])

HSP 1 Score: 1438 bits (3722), Expect = 0.0
Identity = 715/715 (100.00%), Postives = 715/715 (100.00%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR
Sbjct: 74  MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 133

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
           TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW
Sbjct: 134 TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 193

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG
Sbjct: 194 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 253

Query: 181 GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP 240
           GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP
Sbjct: 254 GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP 313

Query: 241 LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI 300
           LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI
Sbjct: 314 LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI 373

Query: 301 QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST 360
           QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST
Sbjct: 374 QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST 433

Query: 361 IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELPRL 420
           IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELPRL
Sbjct: 434 IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELPRL 493

Query: 421 PAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPTVR 480
           PAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPTVR
Sbjct: 494 PAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPTVR 553

Query: 481 PGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTGDT 540
           PGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTGDT
Sbjct: 554 PGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTGDT 613

Query: 541 ILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLLNPS 600
           ILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLLNPS
Sbjct: 614 ILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLLNPS 673

Query: 601 PDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPSGVR 660
           PDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPSGVR
Sbjct: 674 PDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPSGVR 733

Query: 661 VVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT 715
           VVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT
Sbjct: 734 VVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT 788

BLAST of MC02g0007 vs. NCBI nr
Match: XP_023533046.1 (subtilisin-like protease SBT3.18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1080 bits (2792), Expect = 0.0
Identity = 552/723 (76.35%), Postives = 613/723 (84.79%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSYK SFSGFSAKLNASQAM LS+ME VIS+F+SKT++LHTTRSWDFLGLP+P     
Sbjct: 65  MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 124

Query: 61  ----TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPES 120
               TP   +LA G DVVVG+FDSG+WPESESF++   M PVPCSWKGKCVKAYRF+P  
Sbjct: 125 RTFDTPRSSKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 184

Query: 121 ACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVS 180
           ACN KLIGARYYLKGFEA+YG LN SG  NPE F SPRDFLGHGTHTAST VG +VEDVS
Sbjct: 185 ACNRKLIGARYYLKGFEAEYGGLNTSG--NPE-FESPRDFLGHGTHTASTAVGGIVEDVS 244

Query: 181 FLGGGLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLG 240
           F G  LG+GIARGGAPRARLAVYKVCWGKDY+GKCT+AD+MAAFDDAL DGV VISAS G
Sbjct: 245 FTGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFG 304

Query: 241 GTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
           G+PPL PF +SS+ IGSFH MQ+GVSVVFS GNDGP+ SLVQNVSPWSICVAAST DRTF
Sbjct: 305 GSPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTF 364

Query: 301 PTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVL 360
           PTQI I     RISI G+SLIT +I NAKLA+AINYF DG+CER SIRK  KSGAGKVV+
Sbjct: 365 PTQILI---QNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVV 424

Query: 361 CFSTIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTE 420
           CFST+GP S+  AQEA+  INASALIF APPT QLPDLDL+PTVR+DIT ATQIRN L E
Sbjct: 425 CFSTVGPVSMGDAQEALDAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAE 484

Query: 421 LPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETA 480
           LPRLP VEI  A++VIGKS APSVAYFSSRGPSSLSP+ILKPDISAPGVNILAAWPPETA
Sbjct: 485 LPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETA 544

Query: 481 PTVRPGP---KGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATR 540
           PTVRP     +    VKWNFQSGTSMSCPH+SGVVALIKS HP WSPAAIRSALITTAT+
Sbjct: 545 PTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATK 604

Query: 541 RDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQI 600
           RDST +TILAGGS K SDPFD+G GQVNPLKA++PGLVYDMTANDYI FLC++GYTE QI
Sbjct: 605 RDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 664

Query: 601 RMLLNPSPDQAHTPLCCP---SNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYF 660
           RM++NPSP    T +CCP   S  +ANLNYPSITL  L STTTIKRT+RNVA NKNA+YF
Sbjct: 665 RMIVNPSPQ---TLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAENKNAIYF 724

Query: 661 LRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSP 713
           L++ PP+GV+V+VWPRIL FS F Q +SYY+TITPLKK+R RYDFGEI+WS+GFH VTSP
Sbjct: 725 LKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSP 778

BLAST of MC02g0007 vs. NCBI nr
Match: XP_022947798.1 (subtilisin-like protease SBT3.18 [Cucurbita moschata])

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 550/723 (76.07%), Postives = 614/723 (84.92%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSYKHSFSGFSAKLNASQAM LS+ME VIS+F+SKT++LHTTRSWDFLGLP+P    R
Sbjct: 62  MLYSYKHSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGR 121

Query: 61  ----TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPES 120
               TP   +LA G DVVVG+FDSG+WPESESF++   M PVPCSWKGKCVKAYRF+P  
Sbjct: 122 RTFDTPRSRKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 181

Query: 121 ACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVS 180
           ACN KLIGARYYLKGFEA+YG LN SG  NPE F SPRDFLGHGTHTAST VG +VEDVS
Sbjct: 182 ACNRKLIGARYYLKGFEAEYGGLNTSG--NPE-FESPRDFLGHGTHTASTAVGGIVEDVS 241

Query: 181 FLGGGLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLG 240
           F G  LG+GIARGGAPRARLAVYKVCWGKDY+GKCT+AD+MAAFDDAL DGV VISAS G
Sbjct: 242 FTGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFG 301

Query: 241 GTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
           G+PPL PF +SS+ IGSFH MQ+GVSVVFS GNDGP+ SLVQNV PWSICVAAST DRTF
Sbjct: 302 GSPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVFPWSICVAASTMDRTF 361

Query: 301 PTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVL 360
           PTQI I     RISI G+SLIT +I NAKLA+AINYF DG+CER SIRK  KSGAGKVV+
Sbjct: 362 PTQILI---QNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVV 421

Query: 361 CFSTIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTE 420
           CFST+GP S+  AQ+A+  INASALIF APPT QLPDLDL+PTVR+DIT ATQIRN L E
Sbjct: 422 CFSTVGPVSMGDAQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAE 481

Query: 421 LPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETA 480
           LPRLP V+I  A++VIGKS APSVAYFSSRGPSSLSP+ILKPDISAPGVNILAAWPPETA
Sbjct: 482 LPRLPVVQIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETA 541

Query: 481 PTVRPGP---KGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATR 540
           PTVRP     +    VKWNFQSGTSMSCPH+SGVVALIKS HP WSPAAIRSALITTAT+
Sbjct: 542 PTVRPTATEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATK 601

Query: 541 RDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQI 600
           RDST +TILAGGS K SDPFD+G GQVNPLKA++PGLVYDMTANDYI FLC++GYTE QI
Sbjct: 602 RDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 661

Query: 601 RMLLNPSPDQAHTPLCCP---SNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYF 660
           RM++NPS   + T +CCP   S  +ANLNYPSITL  L STTTIKRT+RNVA NKNA+YF
Sbjct: 662 RMIVNPS---SQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYF 721

Query: 661 LRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSP 713
           L++ PP+GV+V+VWPRIL FS F Q +SYY+TITPLKK+R RYDFGEI+WS+GFH VTSP
Sbjct: 722 LKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSP 775

BLAST of MC02g0007 vs. NCBI nr
Match: XP_023007061.1 (subtilisin-like protease SBT3.18 [Cucurbita maxima])

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 550/723 (76.07%), Postives = 614/723 (84.92%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPP-PPT 60
           MLYSYK SFSGFSAKLNASQAM LS+ME VIS+F S+T++LHTTRSWDFLGLP+P    +
Sbjct: 62  MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFMSRTMELHTTRSWDFLGLPIPSYSGS 121

Query: 61  RTPLPPQ---LAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPES 120
           RTP  P+   LA G DVVVG+FDSG+WPESESF++   M PVPCSWKGKCVKAYRF+P  
Sbjct: 122 RTPDTPRSRKLADGDDVVVGIFDSGIWPESESFEDDQWMFPVPCSWKGKCVKAYRFNPAL 181

Query: 121 ACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVS 180
           ACN KLIGARYYLKGFEA+YG LN SG  NPE F SPRDFLGHGTHTAST VG +VEDVS
Sbjct: 182 ACNRKLIGARYYLKGFEAEYGGLNTSG--NPE-FESPRDFLGHGTHTASTAVGGIVEDVS 241

Query: 181 FLGGGLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLG 240
           F G  LG+GIARGGAPRARLAVYKVCWGKDY+GKCT+AD+MAAFDDAL DGV VISAS G
Sbjct: 242 FTGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFG 301

Query: 241 GTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
           G+PPL PF++SS+ IGSFHAMQ+GVSVVFS GNDGP+ SLVQNV PWSICVAAST DRTF
Sbjct: 302 GSPPLAPFIESSSAIGSFHAMQKGVSVVFSAGNDGPYRSLVQNVFPWSICVAASTMDRTF 361

Query: 301 PTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVL 360
           PTQI I     RISI G+SLIT +I NAKLA+AINYF DG+CER SIRK  KSGAGKVV+
Sbjct: 362 PTQILI---QNRISIVGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVV 421

Query: 361 CFSTIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTE 420
           CFST+GP S+  AQEA+  INASALIF APPT QLPDLDL+PTVR+DIT ATQIRN L E
Sbjct: 422 CFSTVGPVSMGDAQEALKAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAE 481

Query: 421 LPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETA 480
           LPRLP VEI  A++VIGKS APSVAYFSSRGPSSLSP+ILKPDISAPGVNILAAWPPETA
Sbjct: 482 LPRLPVVEIRAARSVIGKSAAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETA 541

Query: 481 PTVRPGP---KGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATR 540
           PTVRP     +    VKWNFQSGTSMSCPH+SGVVAL+KS HP WSPAAIRSALITTAT+
Sbjct: 542 PTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALVKSLHPSWSPAAIRSALITTATK 601

Query: 541 RDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQI 600
           RDST +TILAGGS K SDPFD+G GQVNPLKA++PGLVYDMTANDYI FLC++GYTE QI
Sbjct: 602 RDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 661

Query: 601 RMLLNPSPDQAHTPLCCP---SNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYF 660
           RM++NPS     T +CCP   S  +ANLNYPSITL  L STTTIKRT+RNVA NKNA+YF
Sbjct: 662 RMIMNPSQQ---TLVCCPPILSTSIANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYF 721

Query: 661 LRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSP 713
           L++ PP+GV+V+VWPRIL FS F Q +SYY+TITPLKK+  RYDFGEI+WS+GFH VTSP
Sbjct: 722 LKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKAHGRYDFGEIEWSDGFHRVTSP 775

BLAST of MC02g0007 vs. NCBI nr
Match: KAG7035450.1 (Subtilisin-like protease SBT3.18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 549/723 (75.93%), Postives = 612/723 (84.65%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSYK SFSGFSAKLNASQAM LS+ME VIS+F+SKT++LHTTRSWDFLGLP+P     
Sbjct: 64  MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGS 123

Query: 61  ----TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPES 120
               TP   +LA G DVVVG+FDSG+WPESESF++   M PVPCSWKGKCVKAYRF+P  
Sbjct: 124 RTFDTPRSRKLADGADVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 183

Query: 121 ACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVS 180
           ACN KLIGARYYLKGFEA+YG LN SG  NPE F SPRDFLGHGTHTAST VG++VEDVS
Sbjct: 184 ACNRKLIGARYYLKGFEAEYGGLNTSG--NPE-FESPRDFLGHGTHTASTAVGAIVEDVS 243

Query: 181 FLGGGLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLG 240
           F G  LG+GIARGGAP ARLAVYKVCWGKDY+GKCT+AD+MAAFDDAL DGV VISAS G
Sbjct: 244 FTGSSLGKGIARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFG 303

Query: 241 GTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
           G+PPL PF +SS+ IGSFH MQ+GVSVVFS GNDGP+ SLVQNVSPWSICVAAST DRTF
Sbjct: 304 GSPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVSPWSICVAASTMDRTF 363

Query: 301 PTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVL 360
           PTQI I     RISI G+SLIT +I NAKLA+AINYF DG+CER SIRK  KSGAGKVV+
Sbjct: 364 PTQILI---QNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKDRKSGAGKVVV 423

Query: 361 CFSTIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTE 420
           CFST GP S+  AQ+A+  INASALIF APPT QLPDLDL+PTVR+DIT ATQIRN L E
Sbjct: 424 CFSTAGPVSMGDAQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAE 483

Query: 421 LPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETA 480
           LPRLP VEI  A++VIGKS APSVAYFSSRGPSSLSP+ILKPDISAPGVNILAAWPPETA
Sbjct: 484 LPRLPVVEIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETA 543

Query: 481 PTVRPGP---KGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATR 540
           PTVRP     +    VKWNFQSGTSMSCPH+SGVVALIKS HP WSPAAIRSAL+TTAT+
Sbjct: 544 PTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALVTTATK 603

Query: 541 RDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQI 600
           +DST +TILAGGS K SDPFD+G GQVNPLKA++PGLVYDMTANDYI FLC++GYTE QI
Sbjct: 604 KDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 663

Query: 601 RMLLNPSPDQAHTPLCCP---SNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYF 660
           RM++NPSP    T +CCP   S  VANLNYPSITL  L STTTIKRT+RNVA NKNA+YF
Sbjct: 664 RMIVNPSPQ---TLVCCPPFLSTSVANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYF 723

Query: 661 LRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSP 713
           L++ PP+GV+V+VWPRIL FS F Q +SYY+TITPLKK+R RYDFGEI+WS+GFH VTSP
Sbjct: 724 LKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSP 777

BLAST of MC02g0007 vs. ExPASy TrEMBL
Match: A0A6J1DLR1 (subtilisin-like protease SBT3.18 OS=Momordica charantia OX=3673 GN=LOC111021218 PE=3 SV=1)

HSP 1 Score: 1438 bits (3722), Expect = 0.0
Identity = 715/715 (100.00%), Postives = 715/715 (100.00%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR
Sbjct: 74  MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 133

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
           TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW
Sbjct: 134 TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 193

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG
Sbjct: 194 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 253

Query: 181 GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP 240
           GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP
Sbjct: 254 GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP 313

Query: 241 LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI 300
           LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI
Sbjct: 314 LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI 373

Query: 301 QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST 360
           QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST
Sbjct: 374 QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST 433

Query: 361 IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELPRL 420
           IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELPRL
Sbjct: 434 IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELPRL 493

Query: 421 PAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPTVR 480
           PAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPTVR
Sbjct: 494 PAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPTVR 553

Query: 481 PGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTGDT 540
           PGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTGDT
Sbjct: 554 PGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTGDT 613

Query: 541 ILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLLNPS 600
           ILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLLNPS
Sbjct: 614 ILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLLNPS 673

Query: 601 PDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPSGVR 660
           PDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPSGVR
Sbjct: 674 PDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPSGVR 733

Query: 661 VVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT 715
           VVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT
Sbjct: 734 VVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT 788

BLAST of MC02g0007 vs. ExPASy TrEMBL
Match: A0A6J1G7L8 (subtilisin-like protease SBT3.18 OS=Cucurbita moschata OX=3662 GN=LOC111451553 PE=3 SV=1)

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 550/723 (76.07%), Postives = 614/723 (84.92%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSYKHSFSGFSAKLNASQAM LS+ME VIS+F+SKT++LHTTRSWDFLGLP+P    R
Sbjct: 62  MLYSYKHSFSGFSAKLNASQAMTLSKMEGVISIFKSKTMELHTTRSWDFLGLPIPSYSGR 121

Query: 61  ----TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPES 120
               TP   +LA G DVVVG+FDSG+WPESESF++   M PVPCSWKGKCVKAYRF+P  
Sbjct: 122 RTFDTPRSRKLADGDDVVVGIFDSGIWPESESFEDDRWMSPVPCSWKGKCVKAYRFNPSL 181

Query: 121 ACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVS 180
           ACN KLIGARYYLKGFEA+YG LN SG  NPE F SPRDFLGHGTHTAST VG +VEDVS
Sbjct: 182 ACNRKLIGARYYLKGFEAEYGGLNTSG--NPE-FESPRDFLGHGTHTASTAVGGIVEDVS 241

Query: 181 FLGGGLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLG 240
           F G  LG+GIARGGAPRARLAVYKVCWGKDY+GKCT+AD+MAAFDDAL DGV VISAS G
Sbjct: 242 FTGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFG 301

Query: 241 GTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
           G+PPL PF +SS+ IGSFH MQ+GVSVVFS GNDGP+ SLVQNV PWSICVAAST DRTF
Sbjct: 302 GSPPLAPFFESSSAIGSFHGMQKGVSVVFSAGNDGPYRSLVQNVFPWSICVAASTMDRTF 361

Query: 301 PTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVL 360
           PTQI I     RISI G+SLIT +I NAKLA+AINYF DG+CER SIRK  KSGAGKVV+
Sbjct: 362 PTQILI---QNRISIMGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVV 421

Query: 361 CFSTIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTE 420
           CFST+GP S+  AQ+A+  INASALIF APPT QLPDLDL+PTVR+DIT ATQIRN L E
Sbjct: 422 CFSTVGPVSMGDAQQALNAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAE 481

Query: 421 LPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETA 480
           LPRLP V+I  A++VIGKS APSVAYFSSRGPSSLSP+ILKPDISAPGVNILAAWPPETA
Sbjct: 482 LPRLPVVQIRAARSVIGKSVAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETA 541

Query: 481 PTVRPGP---KGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATR 540
           PTVRP     +    VKWNFQSGTSMSCPH+SGVVALIKS HP WSPAAIRSALITTAT+
Sbjct: 542 PTVRPTATEGESQRQVKWNFQSGTSMSCPHISGVVALIKSLHPSWSPAAIRSALITTATK 601

Query: 541 RDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQI 600
           RDST +TILAGGS K SDPFD+G GQVNPLKA++PGLVYDMTANDYI FLC++GYTE QI
Sbjct: 602 RDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 661

Query: 601 RMLLNPSPDQAHTPLCCP---SNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYF 660
           RM++NPS   + T +CCP   S  +ANLNYPSITL  L STTTIKRT+RNVA NKNA+YF
Sbjct: 662 RMIVNPS---SQTLVCCPPFLSTSIANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYF 721

Query: 661 LRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSP 713
           L++ PP+GV+V+VWPRIL FS F Q +SYY+TITPLKK+R RYDFGEI+WS+GFH VTSP
Sbjct: 722 LKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKARGRYDFGEIEWSDGFHRVTSP 775

BLAST of MC02g0007 vs. ExPASy TrEMBL
Match: A0A6J1KZH1 (subtilisin-like protease SBT3.18 OS=Cucurbita maxima OX=3661 GN=LOC111499664 PE=3 SV=1)

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 550/723 (76.07%), Postives = 614/723 (84.92%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPP-PPT 60
           MLYSYK SFSGFSAKLNASQAM LS+ME VIS+F S+T++LHTTRSWDFLGLP+P    +
Sbjct: 62  MLYSYKRSFSGFSAKLNASQAMTLSKMEGVISIFMSRTMELHTTRSWDFLGLPIPSYSGS 121

Query: 61  RTPLPPQ---LAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPES 120
           RTP  P+   LA G DVVVG+FDSG+WPESESF++   M PVPCSWKGKCVKAYRF+P  
Sbjct: 122 RTPDTPRSRKLADGDDVVVGIFDSGIWPESESFEDDQWMFPVPCSWKGKCVKAYRFNPAL 181

Query: 121 ACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVS 180
           ACN KLIGARYYLKGFEA+YG LN SG  NPE F SPRDFLGHGTHTAST VG +VEDVS
Sbjct: 182 ACNRKLIGARYYLKGFEAEYGGLNTSG--NPE-FESPRDFLGHGTHTASTAVGGIVEDVS 241

Query: 181 FLGGGLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLG 240
           F G  LG+GIARGGAPRARLAVYKVCWGKDY+GKCT+AD+MAAFDDAL DGV VISAS G
Sbjct: 242 FTGSSLGKGIARGGAPRARLAVYKVCWGKDYEGKCTDADVMAAFDDALCDGVDVISASFG 301

Query: 241 GTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
           G+PPL PF++SS+ IGSFHAMQ+GVSVVFS GNDGP+ SLVQNV PWSICVAAST DRTF
Sbjct: 302 GSPPLAPFIESSSAIGSFHAMQKGVSVVFSAGNDGPYRSLVQNVFPWSICVAASTMDRTF 361

Query: 301 PTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVL 360
           PTQI I     RISI G+SLIT +I NAKLA+AINYF DG+CER SIRK  KSGAGKVV+
Sbjct: 362 PTQILI---QNRISIVGESLITTNIINAKLANAINYFIDGVCERRSIRKGRKSGAGKVVV 421

Query: 361 CFSTIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTE 420
           CFST+GP S+  AQEA+  INASALIF APPT QLPDLDL+PTVR+DIT ATQIRN L E
Sbjct: 422 CFSTVGPVSMGDAQEALKAINASALIFGAPPTLQLPDLDLLPTVRIDITHATQIRNFLAE 481

Query: 421 LPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETA 480
           LPRLP VEI  A++VIGKS APSVAYFSSRGPSSLSP+ILKPDISAPGVNILAAWPPETA
Sbjct: 482 LPRLPVVEIRAARSVIGKSAAPSVAYFSSRGPSSLSPDILKPDISAPGVNILAAWPPETA 541

Query: 481 PTVRPGP---KGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATR 540
           PTVRP     +    VKWNFQSGTSMSCPH+SGVVAL+KS HP WSPAAIRSALITTAT+
Sbjct: 542 PTVRPTSTEGESQRQVKWNFQSGTSMSCPHISGVVALVKSLHPSWSPAAIRSALITTATK 601

Query: 541 RDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQI 600
           RDST +TILAGGS K SDPFD+G GQVNPLKA++PGLVYDMTANDYI FLC++GYTE QI
Sbjct: 602 RDSTRNTILAGGSTKPSDPFDIGGGQVNPLKAVNPGLVYDMTANDYIIFLCNIGYTEQQI 661

Query: 601 RMLLNPSPDQAHTPLCCP---SNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYF 660
           RM++NPS     T +CCP   S  +ANLNYPSITL  L STTTIKRT+RNVA NKNA+YF
Sbjct: 662 RMIMNPSQQ---TLVCCPPILSTSIANLNYPSITLANLKSTTTIKRTVRNVAANKNAIYF 721

Query: 661 LRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSP 713
           L++ PP+GV+V+VWPRIL FS F Q +SYY+TITPLKK+  RYDFGEI+WS+GFH VTSP
Sbjct: 722 LKLNPPNGVQVLVWPRILLFSWFRQHVSYYITITPLKKAHGRYDFGEIEWSDGFHRVTSP 775

BLAST of MC02g0007 vs. ExPASy TrEMBL
Match: A0A1S3CFU3 (subtilisin-like protease SBT3.18 OS=Cucumis melo OX=3656 GN=LOC103500248 PE=3 SV=1)

HSP 1 Score: 1025 bits (2650), Expect = 0.0
Identity = 531/725 (73.24%), Postives = 602/725 (83.03%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSYK SFSGFSAKLNASQA+ LS+ME VISVF S+T++LHTTRSWDFLGLP+P     
Sbjct: 70  MLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMELHTTRSWDFLGLPIPSYTNN 129

Query: 61  --TPLPPQL-AYG-YDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPES 120
             T  P +L +YG +DVVVG+FDSGVWPES+SF E   +G +PC+WKGKCVK YRF+P S
Sbjct: 130 RSTLFPLRLPSYGDHDVVVGIFDSGVWPESKSFDENEGIGRIPCNWKGKCVKGYRFNPAS 189

Query: 121 ACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVS 180
           ACN KLIGARYYL GFEA+YGALN +   NPE F SPRDFLGHGTHTAST VG+VV +V+
Sbjct: 190 ACNRKLIGARYYLNGFEAQYGALNTTAE-NPE-FRSPRDFLGHGTHTASTAVGAVVHNVA 249

Query: 181 FLGGGL-GRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASL 240
           F    L  +G ARGGAP ARLAVYKVCWGKDY+GKCT+AD+MAAFDDAL DGV VISAS 
Sbjct: 250 FPTSSLLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASF 309

Query: 241 GGTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRT 300
           G TPPL P  +S++ IGSFHAMQ+GVSVVFS GNDGPHPSLVQNVSPWSICVAAST DRT
Sbjct: 310 GETPPLTPLFESASAIGSFHAMQRGVSVVFSAGNDGPHPSLVQNVSPWSICVAASTMDRT 369

Query: 301 FPTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVV 360
           FPT I IL      SI G+SLIT +I N KLADAINYF DGICER +IRK  KSG GKVV
Sbjct: 370 FPTPIFIL---NHFSIMGESLITTNIINVKLADAINYFNDGICERKNIRKGGKSGKGKVV 429

Query: 361 LCFSTIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLT 420
           +CFST+G  SI  AQEA+  INASALIF APPTT+LPDLDLIPTVR+DI +ATQIRN+L 
Sbjct: 430 VCFSTVGQVSIATAQEALKAINASALIFGAPPTTELPDLDLIPTVRIDIDQATQIRNLLA 489

Query: 421 ELPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPET 480
           ELPRLP VEIGVA++VIGKS AP+VAYFSSRGPSS+ P+ILKPDISAPGVNILAAWPPET
Sbjct: 490 ELPRLPMVEIGVARSVIGKSVAPTVAYFSSRGPSSILPDILKPDISAPGVNILAAWPPET 549

Query: 481 APTVRPGPKGSGH---VKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTA- 540
           APTVRP  K +     VKWNFQSGTSMSCPH+SGVVALIKS HP WSPAAIRSA+ITTA 
Sbjct: 550 APTVRPSGKFNEEEEGVKWNFQSGTSMSCPHISGVVALIKSVHPNWSPAAIRSAIITTAA 609

Query: 541 TRRDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEH 600
           T+ D++G+TILAGGSMKASDPFD+GAGQVNP+ A++PGL+YD+T NDYI FLC++GYT+ 
Sbjct: 610 TKVDTSGNTILAGGSMKASDPFDIGAGQVNPIMAINPGLIYDITTNDYITFLCNIGYTDQ 669

Query: 601 QIR-MLLNPSPDQAHTPLCCPSNL--VANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAV 660
           QI  ++LNPSP       CC  +   +AN NYPSITL  L STTTI+R +RNV+ NKNA+
Sbjct: 670 QINNLILNPSPH-----FCCRQSTATIANFNYPSITLANLRSTTTIRRIVRNVSLNKNAI 729

Query: 661 YFLRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVT 713
           YFLRVLPP GVRV VWPR+LFFSCF Q+ISYYVTITPL+KSR RY FGEIQW N FH+VT
Sbjct: 730 YFLRVLPPYGVRVQVWPRVLFFSCFRQQISYYVTITPLRKSRGRYGFGEIQWFNRFHTVT 784

BLAST of MC02g0007 vs. ExPASy TrEMBL
Match: A0A0A0LVP7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G153520 PE=3 SV=1)

HSP 1 Score: 1023 bits (2644), Expect = 0.0
Identity = 529/728 (72.66%), Postives = 597/728 (82.01%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSYK SFSGFSAKLNASQA+ LS+ME VISVF S+T++LHTTRSWDFLGLP+P     
Sbjct: 21  MLYSYKKSFSGFSAKLNASQAIALSKMEDVISVFESRTMKLHTTRSWDFLGLPIPSYTNN 80

Query: 61  TPLPPQL---AYG-YDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPES 120
                 L   +YG +DVVV +FDSGVWPES+SF+E+  +G +PC+WKGKCVK YRF+P S
Sbjct: 81  RSARFSLHLPSYGDHDVVVAIFDSGVWPESKSFEESEGIGRIPCNWKGKCVKGYRFNPAS 140

Query: 121 ACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVS 180
           ACN KLIGARYYLKGFEA+YGALN +   NPE F SPRDFLGHGTHTAST VG+VV +V 
Sbjct: 141 ACNRKLIGARYYLKGFEAQYGALNTTAD-NPE-FRSPRDFLGHGTHTASTAVGAVVHNVG 200

Query: 181 F-LGGGLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASL 240
           F     L +G ARGGAP ARLAVYKVCWGKDY+GKCT+AD+MAAFDDAL DGV VISAS 
Sbjct: 201 FPTSSSLAKGTARGGAPWARLAVYKVCWGKDYEGKCTDADVMAAFDDALHDGVDVISASF 260

Query: 241 GGTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRT 300
           G  PPL P  +S++ IGSFHAMQ+GVSVVFS GNDG HPSLVQNVSPWSICVAAST DRT
Sbjct: 261 GERPPLIPLFESASAIGSFHAMQRGVSVVFSAGNDGSHPSLVQNVSPWSICVAASTMDRT 320

Query: 301 FPTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVV 360
           FPT I IL      SI G+SLIT +I N KLADAINYF DGICER +IRK  KSG GKVV
Sbjct: 321 FPTTIFIL---NHFSIMGESLITRNIINVKLADAINYFNDGICERENIRKGGKSGKGKVV 380

Query: 361 LCFSTIGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLT 420
           +CFSTIG  SI  AQEAV  INASALIF APPTT+LPDLDLIPTVR+DI +ATQIRN L 
Sbjct: 381 VCFSTIGQVSIATAQEAVKAINASALIFGAPPTTELPDLDLIPTVRIDIHQATQIRNFLA 440

Query: 421 ELPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPET 480
           ELPRLP VEIGVA++VIGKS AP+VAYFSSRGPSS+ P+ILKPDISAPGVNILAAWPPET
Sbjct: 441 ELPRLPMVEIGVARSVIGKSVAPTVAYFSSRGPSSILPDILKPDISAPGVNILAAWPPET 500

Query: 481 APTVRPGPKGSGH-------VKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALI 540
           APTVRP  K +         VKWNFQSGTSMSCPHVSGVVALIKS HP WSPAAIRSA+I
Sbjct: 501 APTVRPSGKINEEEEEEEEGVKWNFQSGTSMSCPHVSGVVALIKSVHPNWSPAAIRSAII 560

Query: 541 TTATRRDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGY 600
           TTAT+ DS+G+TILAGGSMKASDPFD+GAGQVNP+ A++PGL+YD+T NDYI FLC++GY
Sbjct: 561 TTATKIDSSGNTILAGGSMKASDPFDIGAGQVNPIMAINPGLIYDITTNDYITFLCNIGY 620

Query: 601 TEHQI-RMLLNPSPDQAHTPLCCPSNL--VANLNYPSITLTRLHSTTTIKRTLRNVAPNK 660
           T+ QI  ++LNPSP       CC  +   +AN NYPSITL  L STTTI+R +RNV+ NK
Sbjct: 621 TDQQISNLILNPSPH-----FCCRQSTATIANFNYPSITLANLRSTTTIRRIVRNVSLNK 680

Query: 661 NAVYFLRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFH 713
           NA+YFLRVLPP GVRV VWPR+LFFSC+ Q+ISYY+TITPL+KSR RY FGEIQW N FH
Sbjct: 681 NAIYFLRVLPPYGVRVQVWPRVLFFSCYRQQISYYITITPLRKSRGRYGFGEIQWFNRFH 738

BLAST of MC02g0007 vs. TAIR 10
Match: AT4G26330.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 815.5 bits (2105), Expect = 3.5e-236
Identity = 428/733 (58.39%), Postives = 537/733 (73.26%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           MLYSY + F GFSAKLN++QA  L+++  VI+VF+SK+L+LHTTRSWDFLGL +     R
Sbjct: 21  MLYSYNNGFLGFSAKLNSTQAASLAKLNQVITVFKSKSLKLHTTRSWDFLGLAV-DNARR 80

Query: 61  TPLPPQLAYGYDVVVGVFDS--------------GVWPESESFQEAWSMGPVPCSWKGKC 120
           TP PPQLAYG D+VVG+FD+              G+WPESESF+E     P+P SW GKC
Sbjct: 81  TP-PPQLAYGSDIVVGIFDTGLFISLKLLLLSILGIWPESESFRETPEAKPIPSSWNGKC 140

Query: 121 VKAYRFDPESACNWKLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTAST 180
           V    FDP   CN KLIGAR+YL+GFE  YG ++ +      E+ SPRD+LGHGTHTAST
Sbjct: 141 VGGEDFDPSVHCNRKLIGARFYLRGFEETYGTIDFT---RDPEYRSPRDYLGHGTHTAST 200

Query: 181 VVGSVVEDVSFLGGGLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRD 240
            VGSVV +VS    GLGRG ARGGAP ARLAV+K CWGKD +G CTEAD++AAFDDA+ D
Sbjct: 201 AVGSVVRNVSGF-FGLGRGTARGGAPLARLAVFKTCWGKDLEGVCTEADILAAFDDAIHD 260

Query: 241 GVHVISASLGGTPPLDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSIC 300
           GVHVISAS G +PPL PF +SSA IG+FHA ++G+SVVFS GNDGP P +VQNV+PW++ 
Sbjct: 261 GVHVISASFGYSPPLSPFFESSADIGAFHAAERGISVVFSTGNDGPDPGVVQNVAPWAVS 320

Query: 301 VAASTTDRTFPTQIQILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKA 360
           VAAST DR+FPT+I I   +   ++TG SLI++ IT   LA A  YF  G+C+  +  K 
Sbjct: 321 VAASTVDRSFPTRIVI---DGSFTLTGQSLISQEITGT-LALATTYFNGGVCKWENWMK- 380

Query: 361 SKSGAGKVVLCFSTIGPTS-IEVAQEAVMTINASALIFAAPPTTQL-PDLDLIPTVRLDI 420
            K     ++LCFST+GP   IE AQ A +  NA ALIFAA PT QL  ++D+IPTVR+DI
Sbjct: 381 -KLANETIILCFSTLGPVQFIEEAQAAAIRANALALIFAASPTRQLAEEVDMIPTVRVDI 440

Query: 421 TRATQIRNVLTELPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPG 480
              T+IRN L   P +P V+IG +K VIG++ APSVAYFSSRGPSSLSP+ILKPDI+APG
Sbjct: 441 LHGTRIRNYLARSPTVPMVKIGPSKTVIGETTAPSVAYFSSRGPSSLSPDILKPDITAPG 500

Query: 481 VNILAAWPPETAPTVRPGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIR 540
           + ILAAWPP T PT+ PG   S  ++WNFQSGTSMSCPHV+GV+AL++SAHP WSP+AIR
Sbjct: 501 IGILAAWPPRTPPTLLPGDHRS--IEWNFQSGTSMSCPHVAGVMALLQSAHPDWSPSAIR 560

Query: 541 SALITTATRRDSTGDTILAGGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLC 600
           SA++TTA  RD++ D IL+GGSMK++DPFD+GAG +NPLKA+DPGLVY+   +DY+ F+C
Sbjct: 561 SAIMTTAYTRDTSYDLILSGGSMKSTDPFDIGAGHINPLKAMDPGLVYNTRTDDYVLFMC 620

Query: 601 SLGYTEHQIR-MLLNPSPDQAHTPLCCPSNLV---ANLNYPSITLTRLHSTTTIKRTLRN 660
           ++GYT+ +I+ M+L+P P    +  C PS+     A+ NYPSIT+  L  T TIKRT+ N
Sbjct: 621 NIGYTDQEIKSMVLHPEP----STTCLPSHSYRTNADFNYPSITIPSLRLTRTIKRTVSN 680

Query: 661 VAPNKNAVYFLRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQW 714
           V PNKN VYF+ ++ P GV V++WPRIL FS   Q  SYYVT  P +    RY FGEI W
Sbjct: 681 VGPNKNTVYFVDIIRPVGVEVLIWPRILVFSKCQQEHSYYVTFKPTEIFSGRYVFGEIMW 735

BLAST of MC02g0007 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 515.0 bits (1325), Expect = 9.7e-146
Identity = 304/716 (42.46%), Postives = 424/716 (59.22%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           ++YSY+H FSGF+AKL  SQA ++S++  V+ V  +   ++ TTR+WD+LG+    P   
Sbjct: 67  IVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGV---SPGNS 126

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
             L  +   GY+V+VGV DSGVWPESE F +    GP+P  WKG C     F+    CN 
Sbjct: 127 DSLLQKANMGYNVIVGVIDSGVWPESEMFNDK-GFGPIPSRWKGGCESGELFNASIHCNR 186

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGA+Y++ G  A++G +N   + NP E+ SPRDF GHGTH AST+ GS + +VS++  
Sbjct: 187 KLIGAKYFVDGLVAEFGVVN--RTQNP-EYLSPRDFAGHGTHVASTIGGSFLPNVSYV-- 246

Query: 181 GLGRGIARGGAPRARLAVYKVCWGKDYQGKCTEADLMAAFDDALRDGVHVISASLGGTPP 240
           GLGRG ARGGAP   +AVYK CW     G C+ AD++ A D+A+ DGV ++S SLG + P
Sbjct: 247 GLGRGTARGGAPGVHIAVYKACW----SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVP 306

Query: 241 LDPFLDSSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTFPTQI 300
           L P  + ++ +G+FHA+ +G+ VV + GN GP    + NV+PW + VAA+T DR+FPT I
Sbjct: 307 LFPETEHTS-VGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAI 366

Query: 301 QILLPNRRISITGDSLITESITNAKLADAINYFTDGICERASIRKASKSGAGKVVLCFST 360
            +      I+I G ++                   G CE+ S    S +  GKVVLCF+ 
Sbjct: 367 TL---GNNITILGQAIYGGPELGFVGLTYPESPLSGDCEKLSANPNS-TMEGKVVLCFAA 426

Query: 361 IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQIRNVLTELPRL 420
             P++  +A  AV+      LI A  PT  L      P V +D    T I   +    R 
Sbjct: 427 STPSNAAIA--AVINAGGLGLIMAKNPTHSLTPTRKFPWVSIDFELGTDILFYIRS-TRS 486

Query: 421 PAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILAAWPPETAPTVR 480
           P V+I  +K + G+S +  VA FSSRGP+S+SP ILKPDI+APGVNILAA  P +  ++ 
Sbjct: 487 PIVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNS--SIN 546

Query: 481 PGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALITTATRRDSTGDT 540
            G        +   SGTSM+ P VSGVV L+KS HP WSP+AI+SA++TTA R D +G+ 
Sbjct: 547 DG-------GFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEP 606

Query: 541 ILA-GGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGYTEHQIRMLLNP 600
           I A G S K +DPFD G G +NP KA+ PGL+YDMT +DY+ ++CS+ Y++  I  +L  
Sbjct: 607 IFADGSSRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGK 666

Query: 601 SPDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAVYFLRVLPPSGV 660
                 T    P   V +LN PSIT+  L    T+ RT+ NV P  N+VY + + PP+G+
Sbjct: 667 I-----TVCPNPKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGP-VNSVYKVVIDPPTGI 726

Query: 661 RVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVTSPLVVNT 716
            V V P  L F   T + S+ V ++   K    Y FG + W++  H+V  P+ V T
Sbjct: 727 NVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVRT 746

BLAST of MC02g0007 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 500.0 bits (1286), Expect = 3.2e-141
Identity = 307/727 (42.23%), Postives = 421/727 (57.91%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           M+YSY+H FSGF+AKL  SQA +L+    V+ V      +L TTR+WD+LGL +  P   
Sbjct: 67  MVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANP--- 126

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
             L      G  V++G  D+GVWPESESF +   +GP+P  WKG C    +F   + CN 
Sbjct: 127 NNLLNDTNMGDQVIIGFIDTGVWPESESFNDN-GVGPIPSHWKGGCESGEKF-ISTNCNR 186

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGA+Y++ GF A+    N + S    ++ S RDF+GHGTHTAS   GS V ++S+   
Sbjct: 187 KLIGAKYFINGFLAENEGFNTTES---RDYISARDFIGHGTHTASIAGGSFVPNISY--K 246

Query: 181 GLGRGIARGGAPRARLAVYKVCWGKDYQG--KCTEADLMAAFDDALRDGVHVISASLGGT 240
           GL  G  RGGAPRAR+A+YK CW  D  G   C+ +D++ A D+++ DGV V+S SLG  
Sbjct: 247 GLAGGNLRGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQ 306

Query: 241 PPLDPFLD--SSAGIGSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
            PL P  D       G+FHA+ +G+ VV +GGN GP    V N +PW I VAA+T DR+F
Sbjct: 307 IPLYPETDLRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSF 366

Query: 301 PTQIQILLPNRRISI-----TGDSLITESITNAKLADAINYFTDGICERASIRKASKSGA 360
           PT   I L NR++ +     TG  L   S+   + A   N    G+CER ++   +++ A
Sbjct: 367 PT--PITLGNRKVILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNL-NPNRTMA 426

Query: 361 GKVVLCFSTIGP-TSIEVAQEAVMTINASALIFAAPPTTQL-PDLDLIPTVRLDITRATQ 420
           GKVVLCF+T    T++  A   V       +I A  P   L P  D  P V +D    T 
Sbjct: 427 GKVVLCFTTNTLFTAVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTD 486

Query: 421 IRNVLTELPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILA 480
           +  +     R P V+I  ++ ++G+     VA FSSRGP+S+SP ILKPDI APGV+ILA
Sbjct: 487 VL-LYIRSTRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILA 546

Query: 481 AWPPETAPTVRPGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALIT 540
           A  P++  +V           ++  +GTSM+ P V+GVVAL+K+ HP WSPAA RSA++T
Sbjct: 547 ATSPDSNSSVG---------GFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVT 606

Query: 541 TATRRDSTGDTILA-GGSMKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGY 600
           TA R D  G+ I A G S K +DPFD G G VNP KA DPGL+YDM   DYI +LCS GY
Sbjct: 607 TAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGY 666

Query: 601 TEHQIRMLLNPSPDQAHTPLCCPSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNAV 660
            +  I  L+        T    P   V ++N PSIT+  L    T+ RT+ NV    ++V
Sbjct: 667 NDSSITQLVGNV-----TVCSTPKTSVLDVNLPSITIPDLKDEVTLTRTVTNVG-TVDSV 726

Query: 661 YFLRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSVT 716
           Y + V PP G++VVV P  L F+  T+ +S+ V ++   K    + FG + W++  H+VT
Sbjct: 727 YKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVT 764

BLAST of MC02g0007 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 6.9e-136
Identity = 303/728 (41.62%), Postives = 413/728 (56.73%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           M+YSY+H FSGF+AKL  SQA +++ +  V+ V      +L TTR+WD+LGL    P + 
Sbjct: 72  MVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS- 131

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
             L  +   G  +++GV D+GVWPESE F ++   GPVP  WKG C     F+  S CN 
Sbjct: 132 --LLHETNMGEQIIIGVIDTGVWPESEVFNDS-GFGPVPSHWKGGCETGENFN-SSNCNK 191

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGA+Y++ GF A+  + N   S N  +F SPRD  GHGTH ++   GS V ++S+   
Sbjct: 192 KLIGAKYFINGFLAENESFN---STNSLDFISPRDLDGHGTHVSTIAGGSFVPNISY--K 251

Query: 181 GLGRGIARGGAPRARLAVYKVCW--GKDYQGKCTEADLMAAFDDALRDGVHVISASLGGT 240
           GL  G  RGGAPRA +A+YK CW    D    C+ AD++ A D+A+ DGV V+S SLG +
Sbjct: 252 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSS 311

Query: 241 PPLDPFLDSSAGI--GSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
            PL    D   GI  G+FHA+ +G++VV SGGN GP    V N +PW I VAA+T DR+F
Sbjct: 312 VPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSF 371

Query: 301 PTQIQILLPNRRISI-----TGDSLITESITNAKLADAINYFTDGICERASIRKASKSGA 360
            T   + L N ++ +     TG  L   S+   +     N    G CE   +  ++++  
Sbjct: 372 AT--PLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEEL-LFNSNRTME 431

Query: 361 GKVVLCFST--IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQ 420
           GKVVLCF+T   G   +  A+          +I   P     P LD  P V +D    T 
Sbjct: 432 GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTD 491

Query: 421 IRNVLTELPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILA 480
           I  + T     P V+I  +K ++G+     VA FSSRGP+S++P ILKPDI+APGV+ILA
Sbjct: 492 IL-LYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILA 551

Query: 481 AWPPETAPTVRPGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALIT 540
           A       T        G +     SGTSM+ P +SGV AL+K+ H  WSPAAIRSA++T
Sbjct: 552 A-------TTNTTFSDQGFI---MLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVT 611

Query: 541 TATRRDSTGDTILAGGS-MKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGY 600
           TA + D  G+ I A GS  K +DPFD G G VNP K+ +PGLVYDM   DY+ ++CS+GY
Sbjct: 612 TAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGY 671

Query: 601 TEHQIRMLLNPSPDQAHTPLCC-PSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNA 660
            E  I  L+        T +C  P   V + N PSIT+  L    TI RT+ NV P  N+
Sbjct: 672 NETSISQLI------GKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGP-LNS 731

Query: 661 VYFLRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSV 716
           VY + V PP G +V V P  L F+  T+++ + V ++   K+   Y FG + WS+  H+V
Sbjct: 732 VYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNV 768

BLAST of MC02g0007 vs. TAIR 10
Match: AT4G10550.2 (Subtilase family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 6.9e-136
Identity = 303/728 (41.62%), Postives = 413/728 (56.73%), Query Frame = 0

Query: 1   MLYSYKHSFSGFSAKLNASQAMELSQMEVVISVFRSKTLQLHTTRSWDFLGLPLPPPPTR 60
           M+YSY+H FSGF+AKL  SQA +++ +  V+ V      +L TTR+WD+LGL    P + 
Sbjct: 16  MVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS- 75

Query: 61  TPLPPQLAYGYDVVVGVFDSGVWPESESFQEAWSMGPVPCSWKGKCVKAYRFDPESACNW 120
             L  +   G  +++GV D+GVWPESE F ++   GPVP  WKG C     F+  S CN 
Sbjct: 76  --LLHETNMGEQIIIGVIDTGVWPESEVFNDS-GFGPVPSHWKGGCETGENFN-SSNCNK 135

Query: 121 KLIGARYYLKGFEAKYGALNVSGSGNPEEFPSPRDFLGHGTHTASTVVGSVVEDVSFLGG 180
           KLIGA+Y++ GF A+  + N   S N  +F SPRD  GHGTH ++   GS V ++S+   
Sbjct: 136 KLIGAKYFINGFLAENESFN---STNSLDFISPRDLDGHGTHVSTIAGGSFVPNISY--K 195

Query: 181 GLGRGIARGGAPRARLAVYKVCW--GKDYQGKCTEADLMAAFDDALRDGVHVISASLGGT 240
           GL  G  RGGAPRA +A+YK CW    D    C+ AD++ A D+A+ DGV V+S SLG +
Sbjct: 196 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSS 255

Query: 241 PPLDPFLDSSAGI--GSFHAMQQGVSVVFSGGNDGPHPSLVQNVSPWSICVAASTTDRTF 300
            PL    D   GI  G+FHA+ +G++VV SGGN GP    V N +PW I VAA+T DR+F
Sbjct: 256 VPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSF 315

Query: 301 PTQIQILLPNRRISI-----TGDSLITESITNAKLADAINYFTDGICERASIRKASKSGA 360
            T   + L N ++ +     TG  L   S+   +     N    G CE   +  ++++  
Sbjct: 316 AT--PLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEEL-LFNSNRTME 375

Query: 361 GKVVLCFST--IGPTSIEVAQEAVMTINASALIFAAPPTTQLPDLDLIPTVRLDITRATQ 420
           GKVVLCF+T   G   +  A+          +I   P     P LD  P V +D    T 
Sbjct: 376 GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTD 435

Query: 421 IRNVLTELPRLPAVEIGVAKNVIGKSGAPSVAYFSSRGPSSLSPEILKPDISAPGVNILA 480
           I  + T     P V+I  +K ++G+     VA FSSRGP+S++P ILKPDI+APGV+ILA
Sbjct: 436 IL-LYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILA 495

Query: 481 AWPPETAPTVRPGPKGSGHVKWNFQSGTSMSCPHVSGVVALIKSAHPKWSPAAIRSALIT 540
           A       T        G +     SGTSM+ P +SGV AL+K+ H  WSPAAIRSA++T
Sbjct: 496 A-------TTNTTFSDQGFI---MLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVT 555

Query: 541 TATRRDSTGDTILAGGS-MKASDPFDVGAGQVNPLKALDPGLVYDMTANDYIPFLCSLGY 600
           TA + D  G+ I A GS  K +DPFD G G VNP K+ +PGLVYDM   DY+ ++CS+GY
Sbjct: 556 TAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGY 615

Query: 601 TEHQIRMLLNPSPDQAHTPLCC-PSNLVANLNYPSITLTRLHSTTTIKRTLRNVAPNKNA 660
            E  I  L+        T +C  P   V + N PSIT+  L    TI RT+ NV P  N+
Sbjct: 616 NETSISQLI------GKTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGP-LNS 675

Query: 661 VYFLRVLPPSGVRVVVWPRILFFSCFTQRISYYVTITPLKKSRPRYDFGEIQWSNGFHSV 716
           VY + V PP G +V V P  L F+  T+++ + V ++   K+   Y FG + WS+  H+V
Sbjct: 676 VYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNV 712