MC02g0003 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g0003
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	MC02: 106241 .. 107853 (+)
RNA-Seq Expression	MC02g0003
Synteny	MC02g0003

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGAGTCAATTCCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCTTTGACGTGGAAATTAAATGCCCCTTCTGCCGTAGTGGGTTTGTTGAAGAGATAGGTAACGGTATTTCGAGTGACAATAATGAGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTTTGGGCCCCGATCTTGCTTGGCATGATGGGAAATTCCCGTGGGCGGCGTAGAATGAGGCATCTAGAGTTTGATGAGGATGAGGAGGAGAATGAGAATGATGATGGGGAGGGGCATGGGCATCGTGTGACTACTGAGGCTACTGAGTTGGACCGAGAGCTTGATTCAATCATTAGGAGGAGGAGAAGTTCAGCTACCATTTTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGAATCAGAGAACTATGAAGGAGACAGGAACAGAGAAAGAGAAAGAGAAAGAGAAAGAGAGCGGGTCATTCTTATAAATCCTTTCAATCAAACCATTGTAGTTCAAGGAGGTCACACTGTGGATCAAAACCCCATGGGGTCGCTGGGTGATTATTTCGTTGGACCTGGTTTAGATTTGTTATTGCAATATCTAGCCGAGAATGATCCCAATAGATATGGGACTCCACCAGCACAGAGGGAGGCAGTGGATGCGCTGCCCACGGTGAGGGTTGAGGATAGTTTGCAGTGTTCGGTGTGTTTGGATGATTTTGAGGTTAACGCAGAAGCCAAGGAGATGCCGTGTAAGCATAAGTTTCATACTGGGTGTATTATGCCGTGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAATCTAAGCGGGATATGGATGGTGGGTCTGGGTCTGGGTCTGCTGCTAGGTTCATGGGTGACCTTAATGTTAATGGTGGTGGCAGTGGTGACATCGAGGCAGGCGTGGATGGCAGAAATGCATCTGGGAGAAGGTTTCCGTTTCCATGGCCCTTCAATGGTTTGTTTTCTTCGCAAGGTGGAGGTGGAGGTGGAGGTGGAGGTGGAAACTCCTGGGAAAATGCAGCTGGAGCAAGTACAAGTGACTCTCAAAGGGATGAGAGTTCCATTTGATCATAGTATCAACTTGTTTCATGTCCTGCTCCTGCTTCTGCCTGCTGAATCAATCTGTAATCTGTATGTATGTATGTATGTATATGTTTGTCTTATACTTTACTATACAGATGTTTTTGCCTCTGACTCCGAGTCCTACCCTTAGAACAGGAATTCACCGCTGCTTTCTTTAATCACCATTCTTCTTTTTTGCCATCTTTAATCACCATCCTAGCGATATGGCTTGGGATTTTGTTTTAAATTTTAATTCTATACCGGGGCAGGGTTTCACCAAAATCACTCCCAAACATTGGTGATGTAAAATAAATAGGCAGATCACTGTAGACTCACACCACAAGCTTGTAATTCAGAAATTGCACTTTGCAAGGCAATATGCTAAATTAATTTCATTTTTTTTAAAGTTTAAGACAAGGAGGATACAATAAGCTTGTCATGCAACGCACAACAATAATTCTTTATTATGAAATATATAGGAATACAAACTTGTG

mRNA sequence

ATGGAGGAGTCAATTCCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCTTTGACGTGGAAATTAAATGCCCCTTCTGCCGTAGTGGGTTTGTTGAAGAGATAGGTAACGGTATTTCGAGTGACAATAATGAGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTTTGGGCCCCGATCTTGCTTGGCATGATGGGAAATTCCCGTGGGCGGCGTAGAATGAGGCATCTAGAGTTTGATGAGGATGAGGAGGAGAATGAGAATGATGATGGGGAGGGGCATGGGCATCGTGTGACTACTGAGGCTACTGAGTTGGACCGAGAGCTTGATTCAATCATTAGGAGGAGGAGAAGTTCAGCTACCATTTTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGAATCAGAGAACTATGAAGGAGACAGGAACAGAGAAAGAGAAAGAGAAAGAGAAAGAGAGCGGGTCATTCTTATAAATCCTTTCAATCAAACCATTGTAGTTCAAGGAGGTCACACTGTGGATCAAAACCCCATGGGGTCGCTGGGTGATTATTTCGTTGGACCTGGTTTAGATTTGTTATTGCAATATCTAGCCGAGAATGATCCCAATAGATATGGGACTCCACCAGCACAGAGGGAGGCAGTGGATGCGCTGCCCACGGTGAGGGTTGAGGATAGTTTGCAGTGTTCGGTGTGTTTGGATGATTTTGAGGTTAACGCAGAAGCCAAGGAGATGCCGTGTAAGCATAAGTTTCATACTGGGTGTATTATGCCGTGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAATCTAAGCGGGATATGGATGGTGGGTCTGGGTCTGGGTCTGCTGCTAGGTTCATGGGTGACCTTAATGTTAATGGTGGTGGCAGTGGTGACATCGAGGCAGGCGTGGATGGCAGAAATGCATCTGGGAGAAGGTTTCCGTTTCCATGGCCCTTCAATGGTTTGTTTTCTTCGCAAGGTGGAGGTGGAGGTGGAGGTGGAGGTGGAAACTCCTGGGAAAATGCAGCTGGAGCAAGTACAAGTGACTCTCAAAGGGATGAGAGTTCCATTTGATCATAGTATCAACTTGTTTCATGTCCTGCTCCTGCTTCTGCCTGCTGAATCAATCTGTAATCTGTATGTATGTATGTATGTATATGTTTGTCTTATACTTTACTATACAGATGTTTTTGCCTCTGACTCCGAGTCCTACCCTTAGAACAGGAATTCACCGCTGCTTTCTTTAATCACCATTCTTCTTTTTTGCCATCTTTAATCACCATCCTAGCGATATGGCTTGGGATTTTGTTTTAAATTTTAATTCTATACCGGGGCAGGGTTTCACCAAAATCACTCCCAAACATTGGTGATGTAAAATAAATAGGCAGATCACTGTAGACTCACACCACAAGCTTGTAATTCAGAAATTGCACTTTGCAAGGCAATATGCTAAATTAATTTCATTTTTTTTAAAGTTTAAGACAAGGAGGATACAATAAGCTTGTCATGCAACGCACAACAATAATTCTTTATTATGAAATATATAGGAATACAAACTTGTG

Coding sequence (CDS)

ATGGAGGAGTCAATTCCAACAAGATATTGGTGTCACATGTGTTCTCAAATGGTGAATCCCATCTTTGACGTGGAAATTAAATGCCCCTTCTGCCGTAGTGGGTTTGTTGAAGAGATAGGTAACGGTATTTCGAGTGACAATAATGAGCTTGAATCCGATTTTGGATCGGAGCGAGCTTTGTCCCTTTGGGCCCCGATCTTGCTTGGCATGATGGGAAATTCCCGTGGGCGGCGTAGAATGAGGCATCTAGAGTTTGATGAGGATGAGGAGGAGAATGAGAATGATGATGGGGAGGGGCATGGGCATCGTGTGACTACTGAGGCTACTGAGTTGGACCGAGAGCTTGATTCAATCATTAGGAGGAGGAGAAGTTCAGCTACCATTTTACAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGAATCAGAGAACTATGAAGGAGACAGGAACAGAGAAAGAGAAAGAGAAAGAGAAAGAGAGCGGGTCATTCTTATAAATCCTTTCAATCAAACCATTGTAGTTCAAGGAGGTCACACTGTGGATCAAAACCCCATGGGGTCGCTGGGTGATTATTTCGTTGGACCTGGTTTAGATTTGTTATTGCAATATCTAGCCGAGAATGATCCCAATAGATATGGGACTCCACCAGCACAGAGGGAGGCAGTGGATGCGCTGCCCACGGTGAGGGTTGAGGATAGTTTGCAGTGTTCGGTGTGTTTGGATGATTTTGAGGTTAACGCAGAAGCCAAGGAGATGCCGTGTAAGCATAAGTTTCATACTGGGTGTATTATGCCGTGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGCGGATGAATCTAAGCGGGATATGGATGGTGGGTCTGGGTCTGGGTCTGCTGCTAGGTTCATGGGTGACCTTAATGTTAATGGTGGTGGCAGTGGTGACATCGAGGCAGGCGTGGATGGCAGAAATGCATCTGGGAGAAGGTTTCCGTTTCCATGGCCCTTCAATGGTTTGTTTTCTTCGCAAGGTGGAGGTGGAGGTGGAGGTGGAGGTGGAAACTCCTGGGAAAATGCAGCTGGAGCAAGTACAAGTGACTCTCAAAGGGATGAGAGTTCCATTTGA

Protein sequence

MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERALSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIRRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHTVDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAARFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNSWENAAGASTSDSQRDESSI

Homology

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BLAST of MC02g0003 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.2e-53
Identity = 136/361 (37.67%), Postives = 191/361 (52.91%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERAL 60
           M E+  TRYWCH C Q +      EIKCP C  GFVEE    ++ +  E  ++   E   
Sbjct: 1   MAEAAITRYWCHECEQAIEEAMVDEIKCPSCGGGFVEE----MTDEEIERLTNRQPEPGF 60

Query: 61  SLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIR 120
           S W PI                   +   E  ++DD          E  +L RE +  IR
Sbjct: 61  SQWNPI-------------------EHPGETMDSDD----------EDNDLGREFEGFIR 120

Query: 121 RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGG-- 180
           R R ++T+ ++L  I   +A                ++ER+  ILIN FNQ + +QG   
Sbjct: 121 RHRRASTLRRVLDSIHDDLAD---------------DQERDSSILINAFNQALALQGSVL 180

Query: 181 --------HTVDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVR 240
                        N  G L +Y +G GL LLLQ+LAE+DP+R GTPPA++EAV+ALPTV+
Sbjct: 181 DPDEGQGDQGGSTNDDGLLEEYVLGAGLSLLLQHLAESDPSRNGTPPAKKEAVEALPTVK 240

Query: 241 VEDSLQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDM 300
           +E+ + CSVCLDD EV ++AK+MPC+HKFH+ CI+PWLELHSSCPVCR +LP++E+K D+
Sbjct: 241 IEEVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSSCPVCRFELPSEETK-DL 300

Query: 301 DGGSGSGSAARFMGDLNVNGGGSGDIEAGVDGRNASGRRFPF-PWPFNGLFSSQGGGGGG 351
           +  S  G       ++  +G G+           +S R +   PW  N LFS++     G
Sbjct: 301 NEPSNIGRVEDSHEEVRADGPGNVS--------ESSNRPWAIVPW-LNELFSTREAQNAG 303

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BLAST of MC02g0003 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.0e-38
Identity = 83/177 (46.89%), Postives = 106/177 (59.89%), Query Frame = 0


Query: 187 GSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSL------QCSVC 246
           G+ GDYF GPGL+ L+Q LAENDPNRYGTPPA + A+DALPTV+V   +      QC+VC
Sbjct: 160 GNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVC 219

Query: 247 LDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAA 306
           +D+FE  ++ K+MPCKH FH  C++PWLELH+SCPVCR +LP D+   +       GS  
Sbjct: 220 MDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYE---NRSQGSQ- 279

Query: 307 RFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNSWE 358
                      GSGD +  V+G+         PWPF     S  G G  G GG + E
Sbjct: 280 -----------GSGDGQGSVEGQQTPRFSIQLPWPFRRQDGSGSGSGAPGTGGGNLE 321

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BLAST of MC02g0003 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.2e-36
Identity = 83/178 (46.63%), Postives = 105/178 (58.99%), Query Frame = 0


Query: 188 SLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDS------LQCSVCL 247
           ++GDYF+GPGL+ L+Q LAENDPNRYGTPPA + A++ALP V +  S       QC+VC+
Sbjct: 169 NIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCM 228

Query: 248 DDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAAR 307
           DDFE   EAK+MPCKH +H  C++PWLELH+SCPVCRH+LP D+   +           R
Sbjct: 229 DDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYE----------RR 288

Query: 308 FMGDLNVNGGGSGDIEA-GVDGRNAS---GRRFPFPWPFNGLFSSQGGGGGGGGGGNS 356
             G    +GG  GD      DG N +     R   PWPF      Q  G G   G N+
Sbjct: 289 VRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRISLPWPF------QARGPGPAPGDNA 330

[Close]

BLAST of MC02g0003 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 9.6e-25
Identity = 98/349 (28.08%), Postives = 142/349 (40.69%), Query Frame = 0


Query: 4   SIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESD--FGSERALS 63
           SI   +WCH C + V  +   +  C +C  GFVEEI  G S  + ++E D  F    A S
Sbjct: 3   SIRNTHWCHRCQRAV-WLRARDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFS 62

Query: 64  LWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIRR 123
            +             R R+    +D                       E+   L S    
Sbjct: 63  AFM------------RSRLAERSYDR----------------------EISGRLGSAGSE 122

Query: 124 RRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHTV 183
             S+   L +  G         +N   +                +N     I +  G   
Sbjct: 123 SFSNLAPLLIFGGQAPFRLAGGDNSSVE--------------AFVNGAAPGIGIARG--- 182

Query: 184 DQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRV------EDSL 243
                 + GDYF GPGL+ L++ L+    +R G PPA + ++DALPT+++          
Sbjct: 183 -----TNAGDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALPTIKITQKHLKSSDS 242

Query: 244 QCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSG 303
            C VC D+FE+ +EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP+           G
Sbjct: 243 HCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS----------RG 271

Query: 304 SGSAARFMGDLNVNGGGSGDIEAGVDGRNASGRR--FPFPWPFNGLFSS 343
           S S+ +   + + N            GR  S RR  F   WPF    SS
Sbjct: 303 SSSSTQSSQNRSTN------------GRENSRRRNIFSNLWPFRSSSSS 271

[Close]

BLAST of MC02g0003 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.8e-23
Identity = 70/177 (39.55%), Postives = 90/177 (50.85%), Query Frame = 0


Query: 187 GSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRV------EDSLQCSVC 246
           G+ GDYF GPGL+ L + L+     R G PPA R A+DALPT+++           C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 247 LDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAA 306
            D+FE+ +EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP+            S S  
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS--------ASGPSSSQN 254

Query: 307 RFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFP--WPFNGLFSSQGGGGGGGGGGNS 356
           R     N     S    +     N + RR PF   WPF    SS       GG  NS
Sbjct: 255 RTTPTRNYRSSSSS--SSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 300

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BLAST of MC02g0003 vs. NCBI nr
Match: XP_022153856.1 (E3 ubiquitin-protein ligase SIRP1-like [Momordica charantia])

HSP 1 Score: 749 bits (1935), Expect = 4.94e-273
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERAL 60
           MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERAL
Sbjct: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERAL 60

Query: 61  SLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIR 120
           SLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIR
Sbjct: 61  SLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIR 120

Query: 121 RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHT 180
           RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHT
Sbjct: 121 RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHT 180

Query: 181 VDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQCSVC 240
           VDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQCSVC
Sbjct: 181 VDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQCSVC 240

Query: 241 LDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAA 300
           LDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAA
Sbjct: 241 LDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAA 300

Query: 301 RFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNSWENAA 360
           RFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNSWENAA
Sbjct: 301 RFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNSWENAA 360

Query: 361 GASTSDSQRDESSI 374
           GASTSDSQRDESSI
Sbjct: 361 GASTSDSQRDESSI 374

[Close]

BLAST of MC02g0003 vs. NCBI nr
Match: XP_022996432.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita maxima] >XP_022996433.1 E3 ubiquitin-protein ligase SIRP1-like [Cucurbita maxima])

HSP 1 Score: 560 bits (1444), Expect = 1.55e-198
Identity = 300/381 (78.74%), Postives = 325/381 (85.30%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNG-ISSDNNELESDFGSERA 60
           M+ESI TRYWCHMCSQMVNPI DVEIKCPFCRSGFVEE+ NG IS DNNELESDFGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSII 120
           LSLWAPILLGMMGN RGRRR RHLEFDED+ +N+ND+GEGH     TE T+LDRELDS++
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHR---LTEPTDLDRELDSVM 120

Query: 121 RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGG- 180
           RRRRSSATILQLLQGIRAGMATESEN+E      RER RERERVILINPFNQTIVVQGG 
Sbjct: 121 RRRRSSATILQLLQGIRAGMATESENFE------RERNRERERVILINPFNQTIVVQGGG 180

Query: 181 -HTVDQN-PMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQ 240
             T +QN P+GSLGDYFVGPGLDLLLQ+LAENDPNRYGTPPA +EAVDALPTVRV+D+LQ
Sbjct: 181 GQTENQNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQ 240

Query: 241 CSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGS 300
           CSVCLDDFEV+AEAKEMPC+HKFHTGCI+PWLELHSSCPVCRHQLPADESKRD+DGG   
Sbjct: 241 CSVCLDDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVDGGG-- 300

Query: 301 GSAARFMGDLNVN--GGGSGDIEAGVDGRN-ASGRRFPFPWPFNGLFSSQGGGGGGGGGG 360
             + RFMGDLNVN  GGG G+IE   DGRN AS  RF FPWPFNGLFSSQ G G      
Sbjct: 301 --STRFMGDLNVNNNGGGFGEIE---DGRNNASESRFSFPWPFNGLFSSQAGAG------ 355

Query: 361 NSWENAAGASTSDSQRDESSI 374
               N++G STSDSQRD+SSI
Sbjct: 361 ----NSSGGSTSDSQRDDSSI 355

[Close]

BLAST of MC02g0003 vs. NCBI nr
Match: XP_022958624.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata] >XP_022958625.1 E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata])

HSP 1 Score: 560 bits (1443), Expect = 2.20e-198
Identity = 299/381 (78.48%), Postives = 325/381 (85.30%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNG-ISSDNNELESDFGSERA 60
           M+ESI TRYWCHMCSQMVNPI DVEIKCPFCRSGFVEE+ NG IS DNNELESDFGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSII 120
           LSLWAPILLGMMGN RGRRR RHLEFDED+ +N+ND+GEGH     TE T+LDRELDS++
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHR---LTEPTDLDRELDSVM 120

Query: 121 RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGG- 180
           RRRRSSATILQLLQGIRAGMATESEN+E      RER RERERVILINPFNQTIVVQGG 
Sbjct: 121 RRRRSSATILQLLQGIRAGMATESENFE------RERNRERERVILINPFNQTIVVQGGG 180

Query: 181 -HTVDQN-PMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQ 240
             T +QN P+GSLGDYFVGPGLDLLLQ+LAENDPNRYGTPPA +EAVDALPTVRV+D+LQ
Sbjct: 181 GQTENQNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQ 240

Query: 241 CSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGS 300
           CSVCLDDFEV+AEAKEMPC+HKFHTGCI+PWLELHSSCPVCRHQLPADESKRD+DGG   
Sbjct: 241 CSVCLDDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVDGGG-- 300

Query: 301 GSAARFMGDLNVN--GGGSGDIEAGVDGRN-ASGRRFPFPWPFNGLFSSQGGGGGGGGGG 360
             + RFMGDLN+N  GGG G+IE   DGRN AS  RF FPWPFNGLFSSQ G G      
Sbjct: 301 --STRFMGDLNINNNGGGFGEIE---DGRNNASESRFSFPWPFNGLFSSQAGAG------ 355

Query: 361 NSWENAAGASTSDSQRDESSI 374
               N++G STSDSQRD+SSI
Sbjct: 361 ----NSSGGSTSDSQRDDSSI 355

[Close]

BLAST of MC02g0003 vs. NCBI nr
Match: XP_023534189.1 (E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558 bits (1437), Expect = 1.80e-197
Identity = 297/381 (77.95%), Postives = 325/381 (85.30%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNG-ISSDNNELESDFGSERA 60
           M+ESI TRYWCHMCSQMVNPI DVEIKCPFCRSGFVEE+ NG +S DNNELESDFGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDVSRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSII 120
           LSLWAPILLGMMGN RGRRR RHLEFDED+ +N+ND+GEGH     TE T+LDRELDS++
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHR---LTEPTDLDRELDSVM 120

Query: 121 RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGG- 180
           RRRRSSATILQLLQGIRAGMATESEN+E      RER RERERVILINPFNQTIVVQGG 
Sbjct: 121 RRRRSSATILQLLQGIRAGMATESENFE------RERNRERERVILINPFNQTIVVQGGG 180

Query: 181 -HTVDQN-PMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQ 240
             T +QN P+GSLGDYFVGPGLDLLLQ+LAENDPNR+GTPPA +EAVDALPTVRV+D+LQ
Sbjct: 181 GQTENQNHPIGSLGDYFVGPGLDLLLQHLAENDPNRHGTPPAHKEAVDALPTVRVKDNLQ 240

Query: 241 CSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGS 300
           CSVCLDDFEV+AEAKEMPC+HKFHTGCI+PWLELHSSCPVCRHQLPADESKRD+DGG   
Sbjct: 241 CSVCLDDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVDGGG-- 300

Query: 301 GSAARFMGDLNVN--GGGSGDIEAGVDGRN-ASGRRFPFPWPFNGLFSSQGGGGGGGGGG 360
             + RFMGDLN+N  GGG G+IE   DGRN AS  RF FPWPFNGLFSSQ G G      
Sbjct: 301 --STRFMGDLNINNNGGGFGEIE---DGRNNASESRFSFPWPFNGLFSSQAGAG------ 355

Query: 361 NSWENAAGASTSDSQRDESSI 374
               N++G STSDSQRD+SSI
Sbjct: 361 ----NSSGGSTSDSQRDDSSI 355

[Close]

BLAST of MC02g0003 vs. NCBI nr
Match: KAG6605826.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560 bits (1443), Expect = 2.59e-197
Identity = 299/381 (78.48%), Postives = 325/381 (85.30%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNG-ISSDNNELESDFGSERA 60
           M+ESI TRYWCHMCSQMVNPI DVEIKCPFCRSGFVEE+ NG IS DNNELESDFGSERA
Sbjct: 69  MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 128

Query: 61  LSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSII 120
           LSLWAPILLGMMGN RGRRR RHLEFDED+ +N+ND+GEGH     TE T+LDRELDS++
Sbjct: 129 LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHR---LTEPTDLDRELDSVM 188

Query: 121 RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGG- 180
           RRRRSSATILQLLQGIRAGMATESEN+E      RER RERERVILINPFNQTIVVQGG 
Sbjct: 189 RRRRSSATILQLLQGIRAGMATESENFE------RERNRERERVILINPFNQTIVVQGGG 248

Query: 181 -HTVDQN-PMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQ 240
             T +QN P+GSLGDYFVGPGLDLLLQ+LAENDPNRYGTPPA +EAVDALPTVRV+D+LQ
Sbjct: 249 GQTENQNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQ 308

Query: 241 CSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGS 300
           CSVCLDDFEV+AEAKEMPC+HKFHTGCI+PWLELHSSCPVCRHQLPADESKRD+DGG   
Sbjct: 309 CSVCLDDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVDGGG-- 368

Query: 301 GSAARFMGDLNVN--GGGSGDIEAGVDGRN-ASGRRFPFPWPFNGLFSSQGGGGGGGGGG 360
             + RFMGDLN+N  GGG G+IE   DGRN AS  RF FPWPFNGLFSSQ G G      
Sbjct: 369 --STRFMGDLNINNNGGGFGEIE---DGRNNASESRFSFPWPFNGLFSSQAGAG------ 423

Query: 361 NSWENAAGASTSDSQRDESSI 374
               N++G STSDSQRD+SSI
Sbjct: 429 ----NSSGGSTSDSQRDDSSI 423

[Close]

BLAST of MC02g0003 vs. ExPASy TrEMBL
Match: A0A6J1DLY0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021272 PE=4 SV=1)

HSP 1 Score: 749 bits (1935), Expect = 2.39e-273
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERAL 60
           MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERAL
Sbjct: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERAL 60

Query: 61  SLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIR 120
           SLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIR
Sbjct: 61  SLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSIIR 120

Query: 121 RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHT 180
           RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHT
Sbjct: 121 RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGGHT 180

Query: 181 VDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQCSVC 240
           VDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQCSVC
Sbjct: 181 VDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQCSVC 240

Query: 241 LDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAA 300
           LDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAA
Sbjct: 241 LDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGSGSAA 300

Query: 301 RFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNSWENAA 360
           RFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNSWENAA
Sbjct: 301 RFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFSSQGGGGGGGGGGNSWENAA 360

Query: 361 GASTSDSQRDESSI 374
           GASTSDSQRDESSI
Sbjct: 361 GASTSDSQRDESSI 374

[Close]

BLAST of MC02g0003 vs. ExPASy TrEMBL
Match: A0A6J1KAR4 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491659 PE=4 SV=1)

HSP 1 Score: 560 bits (1444), Expect = 7.50e-199
Identity = 300/381 (78.74%), Postives = 325/381 (85.30%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNG-ISSDNNELESDFGSERA 60
           M+ESI TRYWCHMCSQMVNPI DVEIKCPFCRSGFVEE+ NG IS DNNELESDFGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSII 120
           LSLWAPILLGMMGN RGRRR RHLEFDED+ +N+ND+GEGH     TE T+LDRELDS++
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHR---LTEPTDLDRELDSVM 120

Query: 121 RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGG- 180
           RRRRSSATILQLLQGIRAGMATESEN+E      RER RERERVILINPFNQTIVVQGG 
Sbjct: 121 RRRRSSATILQLLQGIRAGMATESENFE------RERNRERERVILINPFNQTIVVQGGG 180

Query: 181 -HTVDQN-PMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQ 240
             T +QN P+GSLGDYFVGPGLDLLLQ+LAENDPNRYGTPPA +EAVDALPTVRV+D+LQ
Sbjct: 181 GQTENQNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQ 240

Query: 241 CSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGS 300
           CSVCLDDFEV+AEAKEMPC+HKFHTGCI+PWLELHSSCPVCRHQLPADESKRD+DGG   
Sbjct: 241 CSVCLDDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVDGGG-- 300

Query: 301 GSAARFMGDLNVN--GGGSGDIEAGVDGRN-ASGRRFPFPWPFNGLFSSQGGGGGGGGGG 360
             + RFMGDLNVN  GGG G+IE   DGRN AS  RF FPWPFNGLFSSQ G G      
Sbjct: 301 --STRFMGDLNVNNNGGGFGEIE---DGRNNASESRFSFPWPFNGLFSSQAGAG------ 355

Query: 361 NSWENAAGASTSDSQRDESSI 374
               N++G STSDSQRD+SSI
Sbjct: 361 ----NSSGGSTSDSQRDDSSI 355

[Close]

BLAST of MC02g0003 vs. ExPASy TrEMBL
Match: A0A6J1H411 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459795 PE=4 SV=1)

HSP 1 Score: 560 bits (1443), Expect = 1.06e-198
Identity = 299/381 (78.48%), Postives = 325/381 (85.30%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNG-ISSDNNELESDFGSERA 60
           M+ESI TRYWCHMCSQMVNPI DVEIKCPFCRSGFVEE+ NG IS DNNELESDFGSERA
Sbjct: 1   MDESIATRYWCHMCSQMVNPIIDVEIKCPFCRSGFVEEMNNGDISRDNNELESDFGSERA 60

Query: 61  LSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRELDSII 120
           LSLWAPILLGMMGN RGRRR RHLEFDED+ +N+ND+GEGH     TE T+LDRELDS++
Sbjct: 61  LSLWAPILLGMMGNPRGRRRFRHLEFDEDDGDNDNDEGEGHR---LTEPTDLDRELDSVM 120

Query: 121 RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTIVVQGG- 180
           RRRRSSATILQLLQGIRAGMATESEN+E      RER RERERVILINPFNQTIVVQGG 
Sbjct: 121 RRRRSSATILQLLQGIRAGMATESENFE------RERNRERERVILINPFNQTIVVQGGG 180

Query: 181 -HTVDQN-PMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVEDSLQ 240
             T +QN P+GSLGDYFVGPGLDLLLQ+LAENDPNRYGTPPA +EAVDALPTVRV+D+LQ
Sbjct: 181 GQTENQNHPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAHKEAVDALPTVRVKDNLQ 240

Query: 241 CSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDMDGGSGS 300
           CSVCLDDFEV+AEAKEMPC+HKFHTGCI+PWLELHSSCPVCRHQLPADESKRD+DGG   
Sbjct: 241 CSVCLDDFEVDAEAKEMPCRHKFHTGCILPWLELHSSCPVCRHQLPADESKRDVDGGG-- 300

Query: 301 GSAARFMGDLNVN--GGGSGDIEAGVDGRN-ASGRRFPFPWPFNGLFSSQGGGGGGGGGG 360
             + RFMGDLN+N  GGG G+IE   DGRN AS  RF FPWPFNGLFSSQ G G      
Sbjct: 301 --STRFMGDLNINNNGGGFGEIE---DGRNNASESRFSFPWPFNGLFSSQAGAG------ 355

Query: 361 NSWENAAGASTSDSQRDESSI 374
               N++G STSDSQRD+SSI
Sbjct: 361 ----NSSGGSTSDSQRDDSSI 355

[Close]

BLAST of MC02g0003 vs. ExPASy TrEMBL
Match: A0A6J1FWF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781 PE=4 SV=1)

HSP 1 Score: 527 bits (1358), Expect = 1.52e-185
Identity = 289/394 (73.35%), Postives = 322/394 (81.73%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNN----------EL 60
           M+ESI TRYWCHMCSQMVNPI DVEIKCPFCRSGFVEE+GNG+SSDNN           L
Sbjct: 1   MDESIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEMGNGMSSDNNGNNNNNNSNNAL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDD-GEGHGHRVTTEAT 120
           ESDFGSERALSLWAPILLGMMGN RGRRR RHLEFDED+++N + + G+G G+RV++E T
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPT 120

Query: 121 ELDRELDSIIRRRRSSATILQLLQGIRAGMAT-ESEN-YEGDRNRERERERERER--VIL 180
           ELD     I RRRRSSATILQLLQGIRAGMAT ESEN YEG+R+RERERERERER  VIL
Sbjct: 121 ELD---SVIRRRRRSSATILQLLQGIRAGMATGESENSYEGERSRERERERERERERVIL 180

Query: 181 INPFNQTIVVQGGHTVDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDA 240
           INPFNQTIVVQGG + + N +GSLGDYFVGPGLDLLLQ+LAENDPNRYGTPPAQ+EAVDA
Sbjct: 181 INPFNQTIVVQGGESQNHNAIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDA 240

Query: 241 LPTVRVE---DSLQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLP 300
           LPTVRVE   + LQCSVCLD+FE++ EAKEMPC+HKFH+GCI+PWLELHSSCPVCRHQLP
Sbjct: 241 LPTVRVECGDNRLQCSVCLDEFEMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLP 300

Query: 301 ADESKRDMDGGSGSGSAARFMGDLNVNGGGSGDIEAGVDGRNASG--RRFPFPWPFNGLF 360
           ADESKRD DGGS     ARFMG  + +G GSG+I    DGRNASG  RRF FPWPFNGLF
Sbjct: 301 ADESKRDADGGS-----ARFMGSGSGSGSGSGEI----DGRNASGTGRRFSFPWPFNGLF 360

Query: 361 SSQGGGGGGGGGGNSWENAAGASTSDSQRDESSI 374
           SSQG             N++G S S+SQRD +SI
Sbjct: 361 SSQG-------------NSSGGSGSESQRDHTSI 369

[Close]

BLAST of MC02g0003 vs. ExPASy TrEMBL
Match: A0A0A0KWR3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE=4 SV=1)

HSP 1 Score: 523 bits (1348), Expect = 3.93e-184
Identity = 292/395 (73.92%), Postives = 321/395 (81.27%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNN----------EL 60
           M+E I TRYWCHMCSQMVNPI DVEIKCPFCRSGFVEEIGNGISSDNN          EL
Sbjct: 1   MDELIATRYWCHMCSQMVNPIVDVEIKCPFCRSGFVEEIGNGISSDNNDNNNNNNNNNEL 60

Query: 61  ESDFGSERALSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATE 120
           ESDFGSERALSLWAPILLGMMGN RGRRR RHLEFD+D+++NE++DGEG+  RV++E TE
Sbjct: 61  ESDFGSERALSLWAPILLGMMGNPRGRRRFRHLEFDDDDDDNEHEDGEGN--RVSSETTE 120

Query: 121 LDRELDSIIRRRRSSATILQLLQGIRAGMAT-ESEN-YEGDRNRERERERERERVILINP 180
           LD    S+IRRRRSSATILQLLQGIRAG+AT ESEN YEG+R+RERER      VILINP
Sbjct: 121 LD----SVIRRRRSSATILQLLQGIRAGIATAESENSYEGERSRERER------VILINP 180

Query: 181 FNQTIVVQGGHTVDQNP--MGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDAL 240
           FNQTIVVQGG   +QN   +GSLGDYFVGPGLDLLLQ++AENDPNRYGTPPAQ+EAVDAL
Sbjct: 181 FNQTIVVQGGENQNQNQNSIGSLGDYFVGPGLDLLLQHIAENDPNRYGTPPAQKEAVDAL 240

Query: 241 PTVRVE---DS-LQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLP 300
           PTVRVE   DS LQCSVCLD+FEV+ EAKEMPCKHKFHTGCI+PWLELHSSCPVCRHQLP
Sbjct: 241 PTVRVELEEDSCLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLP 300

Query: 301 ADESKRDMDGGSGSGSAARFMGDLNV-NGGGSGDIEAGVDGRNAS--GRRFPFPWPFNGL 360
            DESKRD+DGGS    AARFM DLN  NG GSG+IE    GRN+S  GRRF FPWPFNGL
Sbjct: 301 GDESKRDVDGGS----AARFMSDLNNGNGNGSGEIE----GRNSSESGRRFSFPWPFNGL 360

Query: 361 FSSQGGGGGGGGGGNSWENAAGASTSDSQRDESSI 374
           F+SQG             N++  S SDSQRD +SI
Sbjct: 361 FTSQG-------------NSSAGSASDSQRDHTSI 362

[Close]

BLAST of MC02g0003 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 371.3 bits (952), Expect = 9.0e-103
Identity = 212/400 (53.00%), Postives = 263/400 (65.75%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEI-GNGISS------DNNELESD 60
           MEE++  RYWCHMCSQMVNP+ + EIKCPFC+SGF+EE+ GNG         D  + E+D
Sbjct: 1   MEETMAARYWCHMCSQMVNPVMESEIKCPFCQSGFIEEMSGNGGGGGGRGIRDVQDSETD 60

Query: 61  FGSERALSLWAPILLGMMGNSRGRRRMRHLEFDEDEEENEND-----DGEGHG------- 120
           FG++RALSLWAPILLGMM + R RRR R  EF E+ ++N +D     DG G+        
Sbjct: 61  FGTDRALSLWAPILLGMMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNNSNNN 120

Query: 121 ----HRVTTEATE--LDRELDSII-RRRRSSATILQLLQGIRAGMATESENYEGDRNRER 180
               HR      E  LDRE +SI+ RRRRSS  ILQLLQGIRAG+A+E   YE   N   
Sbjct: 121 VYRHHRARRHGGEIDLDREFESILRRRRRSSGNILQLLQGIRAGIASE---YESSDN-NW 180

Query: 181 ERERERERVILINPFNQTIVVQGGHTVDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYG 240
           +  RER+RVI+INP+NQ++VV      +   + SLGDYF+GPGLDLLLQ+LAENDPNR G
Sbjct: 181 DNSRERDRVIMINPYNQSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNRQG 240

Query: 241 TPPAQREAVDALPTVRVEDSLQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSC 300
           TPPA++EAV+ALPTV++ + LQCSVCLDDFE   EAKEMPCKHKFH  CI+PWLELHSSC
Sbjct: 241 TPPARKEAVEALPTVKIMEPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLELHSSC 300

Query: 301 PVCRHQLPA-----DESKRDMDGGSGSGSAARFMGDLNVNGGGSGDIEAGVDGRNASGRR 360
           PVCR +LP+     DE+K D +    + +         V   G+ D     + R+ +GRR
Sbjct: 301 PVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGREDEVRSGNGRR 360

Query: 361 FPFPWPFNGLFSSQGGGGGGGGGGNSWENAAGASTSDSQR 370
           F FPWPF+GLFSS         G  S EN   + +S S R
Sbjct: 361 FSFPWPFSGLFSSSSSSSSSTSGSQSGENNFFSRSSGSSR 396

[Close]

BLAST of MC02g0003 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 340.1 bits (871), Expect = 2.2e-93
Identity = 190/361 (52.63%), Postives = 233/361 (64.54%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVEIKCPFCRSGFVEEIGNGISSDNNELESDFGSERA- 60
           MEE++ TRYWCHMCSQ VNP+ + EIKCPFC+SGFVEE    +  D++   SD    RA 
Sbjct: 1   MEEAVVTRYWCHMCSQTVNPVMEAEIKCPFCQSGFVEE----MEDDDDHDSSDPADVRAN 60

Query: 61  LSLWAPILLGMMGNSRGRRRMRHLEFDEDEE-----ENENDDGEGHGHRVTTEATELDRE 120
            SLWAPIL+ +M +   RRR + +E  ED +     EN  DDGE           +LD +
Sbjct: 61  NSLWAPILMELMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE----------NDLDWQ 120

Query: 121 LDSII-RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTI 180
           L  I+ RRRR SA +LQLLQGIRAG++ ESE+     N          RVILIN  NQTI
Sbjct: 121 LQEILRRRRRHSAAVLQLLQGIRAGLSVESESTGNGGNNP-------GRVILINTSNQTI 180

Query: 181 VVQGGHTVDQNPMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQREAVDALPTVRVED 240
            VQ    +D  P GSLGDYF+GPG ++LLQ LAENDPNRYGTPPA++EAV+AL TV++E+
Sbjct: 181 TVQNSADMDSVPAGSLGDYFIGPGFEMLLQRLAENDPNRYGTPPAKKEAVEALATVKIEE 240

Query: 241 SLQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPADESKRDM--- 300
           +LQCSVCLDDFE+  EAK MPC HKFH+ C++PWLELHSSCPVCR+QLPADE+K D    
Sbjct: 241 TLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTDSVTT 300

Query: 301 ---DGGSGSGSAARFMGDLNVNGG------GSGDIEAGVDGRNASGRRFPFPWPFNGLFS 343
              + GS S SA    G  N +G          + E   +  N  G  F  PWPF+ LFS
Sbjct: 301 TSDNNGSSSASATTSHGAENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIPWPFSTLFS 340

[Close]

BLAST of MC02g0003 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.7e-88
Identity = 186/373 (49.87%), Postives = 236/373 (63.27%), Query Frame = 0


Query: 8   RYWCHMCSQMVNPIFDVEIKCPFCRSGFVE----EIGNGISSDNNEL---ESDFGSERAL 67
           RYWCHMCSQMVNP+   EIKCPFC+SGFVE    EI  G SS   E+   E DFG++RAL
Sbjct: 4   RYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREINGGSSSSLREVQDPEIDFGTDRAL 63

Query: 68  SLWAPILLGMMGNSRGRRRMRHLEFDEDEEE-NENDDGEG-------------HGHRVTT 127
           SLW PILLGMM N R RRR R  EF  D +E N +DD +G             H HR   
Sbjct: 64  SLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRHRHRQQG 123

Query: 128 EATELDRELDSII-RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVIL 187
              +LDRE +SI+ RRRRSSATILQLLQGIRAG+A+E E+ + D                
Sbjct: 124 REIDLDREFESILRRRRRSSATILQLLQGIRAGIASEYESSDRDL--------------- 183

Query: 188 INPFNQTIVVQGGHTVDQN----PMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQRE 247
               NQ+ VVQG  +++QN     + ++GDYFVG  LD LL++LA+ND  R+G+ PA++E
Sbjct: 184 ---LNQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPARKE 243

Query: 248 AVDALPTVRVEDSLQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQL 307
            VD LPTV++ +SLQCS+CLDDF+  +EAKEMPCKHKFH  CI+PWLELHSSCPVCR++L
Sbjct: 244 VVDNLPTVKISESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYEL 303

Query: 308 PADESKRDMDGGSGSGSAARFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFS 355
           P D+  +       + +      ++NV+   + ++E      N S RRF FPWPF+GLFS
Sbjct: 304 PPDDETKVNPVRPRTRTL-----EINVS---NENVEDDARNSNVSERRFSFPWPFSGLFS 350

[Close]

BLAST of MC02g0003 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.7e-88
Identity = 186/373 (49.87%), Postives = 236/373 (63.27%), Query Frame = 0


Query: 8   RYWCHMCSQMVNPIFDVEIKCPFCRSGFVE----EIGNGISSDNNEL---ESDFGSERAL 67
           RYWCHMCSQMVNP+   EIKCPFC+SGFVE    EI  G SS   E+   E DFG++RAL
Sbjct: 4   RYWCHMCSQMVNPVIGAEIKCPFCQSGFVEEMSREINGGSSSSLREVQDPEIDFGTDRAL 63

Query: 68  SLWAPILLGMMGNSRGRRRMRHLEFDEDEEE-NENDDGEG-------------HGHRVTT 127
           SLW PILLGMM N R RRR R  EF  D +E N +DD +G             H HR   
Sbjct: 64  SLWGPILLGMMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRHRHRQQG 123

Query: 128 EATELDRELDSII-RRRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVIL 187
              +LDRE +SI+ RRRRSSATILQLLQGIRAG+A+E E+ + D                
Sbjct: 124 REIDLDREFESILRRRRRSSATILQLLQGIRAGIASEYESSDRDL--------------- 183

Query: 188 INPFNQTIVVQGGHTVDQN----PMGSLGDYFVGPGLDLLLQYLAENDPNRYGTPPAQRE 247
               NQ+ VVQG  +++QN     + ++GDYFVG  LD LL++LA+ND  R+G+ PA++E
Sbjct: 184 ---LNQSAVVQGSTSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPARKE 243

Query: 248 AVDALPTVRVEDSLQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQL 307
            VD LPTV++ +SLQCS+CLDDF+  +EAKEMPCKHKFH  CI+PWLELHSSCPVCR++L
Sbjct: 244 VVDNLPTVKISESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYEL 303

Query: 308 PADESKRDMDGGSGSGSAARFMGDLNVNGGGSGDIEAGVDGRNASGRRFPFPWPFNGLFS 355
           P D+  +       + +      ++NV+   + ++E      N S RRF FPWPF+GLFS
Sbjct: 304 PPDDETKVNPVRPRTRTL-----EINVS---NENVEDDARNSNVSERRFSFPWPFSGLFS 350

[Close]

BLAST of MC02g0003 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.8e-71
Identity = 156/304 (51.32%), Postives = 193/304 (63.49%), Query Frame = 0


Query: 1   MEESIPTRYWCHMCSQMVNPIFDVE-IKCPFCRSGFVEEIGNGISSDNNELESDFGSERA 60
           ME++  T YWCHMCS+ V P+   E IKC FC+SGFVEE+      DNN+       + A
Sbjct: 1   MEDASETSYWCHMCSRSVIPLIQDEIIKCNFCQSGFVEEM------DNND-----DHQAA 60

Query: 61  LSLWAPILLGMMGN-----SRGRRRMRHLEFDEDEEENENDDGEGHGHRVTTEATELDRE 120
            SLW PIL+ MM N     S  +     +  DEDE+E++ DDG+ +         ++  +
Sbjct: 61  DSLWTPILMEMMNNNHDQHSTNQEDSESILEDEDEDEDDGDDGDQN----NDGEIDITHQ 120

Query: 121 LDSIIR-RRRSSATILQLLQGIRAGMATESENYEGDRNRERERERERERVILINPFNQTI 180
           L+ I R R R S  I+ LLQGIRAG+  ESEN E + +         E V+LIN FNQ I
Sbjct: 121 LEEIRRIRTRHSTAIVNLLQGIRAGLLIESENNEDNPDNS-------ELVVLINSFNQRI 180

Query: 181 -VVQGGHTVDQNPMGSLGDYFVGPGLDLLLQYLAEND-PNRYGTPPAQREAVDALPTVRV 240
            V Q        P GSLGDYF+GPG + LLQ LAEND  NRYGTPPA +EAV+AL  V++
Sbjct: 181 RVHQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTPPATKEAVEALAMVKI 240

Query: 241 EDS-LQCSVCLDDFEVNAEAKEMPCKHKFHTGCIMPWLELHSSCPVCRHQLPA---DESK 292
           EDS LQCSVCLDDFE+  EAKEMPCKHKFH+ C++PWLELHSSCPVCR+ LP    DE K
Sbjct: 241 EDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPTGDDDEPK 282

The following BLAST results are available for this feature:

BLAST of MC02g0003 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AVN2	2.2e-53	37.67	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8LPN7	2.0e-38	46.89	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	4.2e-36	46.63	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q94AK4	9.6e-25	28.08	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
O22197	1.8e-23	39.55	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of MC02g0003 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153856.1	4.94e-273	100.00	E3 ubiquitin-protein ligase SIRP1-like [Momordica charantia]	[more]
XP_022996432.1	1.55e-198	78.74	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita maxima] >XP_022996433.1 E3 ubi...	[more]
XP_022958624.1	2.20e-198	78.48	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita moschata] >XP_022958625.1 E3 u...	[more]
XP_023534189.1	1.80e-197	77.95	E3 ubiquitin-protein ligase SIRP1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6605826.1	2.59e-197	78.48	E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MC02g0003 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLY0	2.39e-273	100.00	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102127...	[more]
A0A6J1KAR4	7.50e-199	78.74	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491659 P...	[more]
A0A6J1H411	1.06e-198	78.48	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459795...	[more]
A0A6J1FWF9	1.52e-185	73.35	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781...	[more]
A0A0A0KWR3	3.93e-184	73.92	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE...	[more]

BLAST of MC02g0003 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56340.1	9.0e-103	53.00	RING/U-box superfamily protein	[more]
AT1G55530.1	2.2e-93	52.63	RING/U-box superfamily protein	[more]
AT4G26400.2	1.7e-88	49.87	RING/U-box superfamily protein	[more]
AT4G26400.1	1.7e-88	49.87	RING/U-box superfamily protein	[more]
AT3G13430.1	1.8e-71	51.32	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 237..277 e-value: 6.6E-8 score: 42.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 236..278 e-value: 4.7E-13 score: 49.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 237..278 score: 12.386007
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 205..284 e-value: 1.7E-20 score: 74.5
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 7..39 e-value: 2.0E-11 score: 43.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 336..374
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 284..324
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 85..106
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 357..374
None	No IPR available	PANTHER	PTHR15710:SF22	RING/U-BOX SUPERFAMILY PROTEIN	coord: 2..337
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 2..337
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 236..278 e-value: 3.91989E-22 score: 86.2176
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 226..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g0003.1	MC02g0003.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity