MC01g_new0485 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0485 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 8.4e-211
Identity = 353/522 (67.62%), Postives = 434/522 (83.14%), Query Frame = 0

Query: 12  SLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPF-SSFCAPN-NAT-FNALLQSTAQ 71
           +LFLV LL  S  + S++L++ F++CL +NS  S P  +SF +P+ NAT F   L+STAQ
Sbjct: 11  TLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQ 70

Query: 72  NLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASETETSF 131
           NLRYL  S PKP+FIF P Y++HVQAAV+C+K+L++HLR+RSGGHDYEGLS+ +E ET F
Sbjct: 71  NLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPF 130

Query: 132 MILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITG 191
           +I+DL+KLR + VD++ NSAW  AGATIGEVYYRI EKS+ HGFPAGLC+SLG+GGH+ G
Sbjct: 131 VIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVG 190

Query: 192 GAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILWWKLN 251
           GAYG MMRK+GLGADNV+DARIVDANG+ILDR AMGEDVFWAIRGGGGGSFG+IL WK+ 
Sbjct: 191 GAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIK 250

Query: 252 LVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADK--KKTVS 311
           LVPVP+TVTVFTV KTLEQ  TK+LYKW+Q+ADKLD+DLFIRVII+ A+   K   +T+S
Sbjct: 251 LVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTIS 310

Query: 312 TAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLLQGK 371
            +Y A FLGD+NRLL+VM +SFPELGLT+KDC EMSWIKSV+YIAGFP+   PE LL GK
Sbjct: 311 MSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGK 370

Query: 372 STFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPFPHR 431
           S FKN+FKAKSDFVKEPI   GLEGLW+R  EE+SPLTIWNPYGGMM +ISE+EIPFPHR
Sbjct: 371 SLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHR 430

Query: 432 RGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKKG 491
            G LFKIQ+L+ WQ G  ++E+H++WI+++Y+YM  YVS+ PR+AYVNYRDLDLG N +G
Sbjct: 431 NGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTN-EG 490

Query: 492 NTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIP 529
            T   DA  WG +Y+K NF RL+++K + DPDNFFRHEQS+P
Sbjct: 491 ET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of MC01g_new0485 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.1e-209
Identity = 354/519 (68.21%), Postives = 420/519 (80.92%), Query Frame = 0

Query: 15  LVFLLSSSCWVDSASLEESFLQCLNENSQFSVPF-SSFCAP--NNATFNALLQSTAQNLR 74
           L+ L  S   +  +++++ F+ CL +NS  S P  SSF     N ++F   L+++AQNLR
Sbjct: 16  LLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLR 75

Query: 75  YLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASETETSFMIL 134
           YL  S PKP FIF P Y++HVQAAV+C+K+LK+HLR+RSGGHDYEGLSY SE ET+F+I+
Sbjct: 76  YLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIV 135

Query: 135 DLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAY 194
           DL+KLR I VDIE NSAWV AGA+IGEVYYRI EKSK+HGFPAGLCTSLG+GGHI GGAY
Sbjct: 136 DLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAY 195

Query: 195 GPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILWWKLNLVP 254
           G MMRK+GLGADNV+DARIVDA+G+IL+R AMGEDVFWAIRGGGGGSFG+IL WK+ LVP
Sbjct: 196 GSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVP 255

Query: 255 VPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKK--TVSTAY 314
           VP  VTVFTV +TLEQ  TK+LYKWQQVADKLDEDLFIRVII   +   K K  T+ST+Y
Sbjct: 256 VPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSY 315

Query: 315 NALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLLQGKSTF 374
              FLGDANRLL+VM  SFP+LGLT+KDC+E SWIKSV+YIAGFPS  P E LL GKS F
Sbjct: 316 QGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLF 375

Query: 375 KNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPFPHRRGI 434
           KNYFKAKSD+V+EPI   GLEGLW++L EE+SPLTIWNPYGGMM KI ETE PFPHR G 
Sbjct: 376 KNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFPHRSGT 435

Query: 435 LFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKKGNTS 494
           LFKIQ+LTLWQ G +++ KH+ W++++Y+YM  YVS+ PR AYVNYRDLDLG+N KG+  
Sbjct: 436 LFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKGS-- 495

Query: 495 YIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIP 529
             DA  WG RYFK NF RL+ +K K DP+NFFRHEQSIP
Sbjct: 496 --DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of MC01g_new0485 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 1.9e-199
Identity = 333/509 (65.42%), Postives = 410/509 (80.55%), Query Frame = 0

Query: 28  ASLEESFLQCLNENSQFSVPF-SSFCAP--NNATFNALLQSTAQNLRYLETSVPKPLFIF 87
           ASL++ F+ C+  N+    P   +F AP  N + F+ +L+STAQNLR+L+ S+PKP FIF
Sbjct: 29  ASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIF 88

Query: 88  TPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASETETSFMILDLAKLRSIKVDIE 147
           +P ++SHVQA++ICSK+L++HLRVRSGGHDYEGLSY S+ +  F+++DL+K+R + ++I+
Sbjct: 89  SPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQ 148

Query: 148 GNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAYGPMMRKYGLGADN 207
            NSAWVQ+GAT+GE+YYRIAEKSKVHGFPAGLC+SLG+GGHITGGAYG MMRKYGLGADN
Sbjct: 149 DNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADN 208

Query: 208 VVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILWWKLNLVPVPSTVTVFTVAKT 267
           V+DA+IVDANG++LDR AMGED FWAIRGG GGSFGIIL WK+ LVPVP TVTVFTV KT
Sbjct: 209 VLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKT 268

Query: 268 LEQG-ATKILYKWQQVADKLDEDLFIRVIINVAANADKKKTVSTAYNALFLGDANRLLKV 327
           L+Q    KI+ KWQ+VADKL E+LFIRV+ NVA      KTV+T+YNALFLG    L+ V
Sbjct: 269 LQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGG-NKTVTTSYNALFLGGKGTLMNV 328

Query: 328 MGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLLQGKSTF-KNYFKAKSDFVKE 387
           M +SFPELGLT KDCIEMSW++S+ YI+GFP+ TP  VLLQGKS F K  FKAKSDFVK 
Sbjct: 329 MKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKT 388

Query: 388 PISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPFPHRRGILFKIQYLTLWQKG 447
           PI E+GL+G++K+L +E+ PL IWNPYGGMM KI E++IPFPHR+G+LFK+QY+T W   
Sbjct: 389 PIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDS 448

Query: 448 DSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKKG-NTSYIDAIGWGTRYF 507
           D    +H+ WI+ LY+YMTPYVS  PREAYVNYRDLDLG N K   T    A  WG  YF
Sbjct: 449 DKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYF 508

Query: 508 KENFGRLLRVKTKVDPDNFFRHEQSIPPI 531
           K NF RL+ +K KVDP+NFFRHEQSIPP+
Sbjct: 509 KNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of MC01g_new0485 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 6.0e-185
Identity = 319/532 (59.96%), Postives = 399/532 (75.00%), Query Frame = 0

Query: 8   SVVGSLFLVFLLSSSCWVDSASLEESFLQCL--NENSQFSVPFSSFCAPNNAT-FNALLQ 67
           S +    L F   +     S SL++ F++CL  N N +F++  + F    NA+ F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 68  STAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASET 127
           STAQN RYL  ++PKP FIF P ++SHVQA+VICSK+L+IH RVRSGGHDYEG+SY S+ 
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 128 ETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 187
           E  F+++DL+KLR I VDI+  SAWV+AGAT+GE+YYRIAEKSK HGFPAG+  SLG+GG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 188 HITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILW 247
           HITGGAYG +MRKYGL ADNV+DA+IVDANG++LDR +MGED+FWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 248 WKLNLVPVPSTVTVFTVAKTLEQGAT-KILYKWQQVADKLDEDLFIRVIINVAANADKKK 307
           WK+ LVPVP T+TVFTV KT EQ  + KIL KWQ++AD L ++LF+RV   V+ N    K
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN-KANK 310

Query: 308 TVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPP--EV 367
           TV+ AY   FLG+   L++VM + FPELGLT+KDCIEMSWI S++Y +GFP+  PP  E+
Sbjct: 311 TVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEI 370

Query: 368 LLQGKSTF-KNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETE 427
           LLQ KS   K YFK KSDF K+PI   GLEG++K+L EE++ L IW PYGG M KI E+E
Sbjct: 371 LLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESE 430

Query: 428 IPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDL 487
           IPFPHR G  F IQY   W   +    +  +WI++LY YMTPYVS  PR+AYVNYRDLDL
Sbjct: 431 IPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDL 490

Query: 488 GINKKGNTS-YIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIP 532
           G NK  + S +I+A  WG  YFK+NF RL+R+K+KVDPDNFFRHEQSIP +P
Sbjct: 491 GQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLP 541

BLAST of MC01g_new0485 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 5.1e-184
Identity = 309/511 (60.47%), Postives = 386/511 (75.54%), Query Frame = 0

Query: 27  SASLEESFLQCLNENSQFSVPFSSFC---APNNATFNALLQSTAQNLRYLETSVPKPLFI 86
           SAS+++ F+ C+  N+  S P        A N + FN +L+STAQNL++L  S+PKP FI
Sbjct: 31  SASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKPGFI 90

Query: 87  FTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASETETSFMILDLAKLRSIKVDI 146
           F P + S VQA++ICSK+L IH RVRSGGHD+E LSY S  E  F++LDL+KL+ I VDI
Sbjct: 91  FRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQINVDI 150

Query: 147 EGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAYGPMMRKYGLGAD 206
           E NSAWVQ GAT+GE+YYRIAEKSK+HGFPAGLCTS+G+GG++TGG YG +MRKYGL  D
Sbjct: 151 ESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGLAGD 210

Query: 207 NVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILWWKLNLVPVPSTVTVFTVAK 266
           NV+D ++VDANG++LDR AMGED+FWAIRGGGG SFGI+L WK+ LVPVP TVTVFTV K
Sbjct: 211 NVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFTVTK 270

Query: 267 TLEQGA-TKILYKWQQVADKLDEDLFIRVIINVAANADKKKTVSTAYNALFLGDANRLLK 326
           TLEQ A  K + KWQQ++ K+ E++ IRV++  A N D  KTV+  Y   FLG+   LLK
Sbjct: 271 TLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN-DGNKTVTMTYLGQFLGEKGTLLK 330

Query: 327 VMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLLQGKSTF-KNYFKAKSDFVK 386
           VM ++FPELGLT+KDC EMSWI++ L+  GFP+G+P E+LLQ KS   K+YFKA SDFVK
Sbjct: 331 VMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKSPLGKDYFKATSDFVK 390

Query: 387 EPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPFPHRRGILFKIQYLTLWQK 446
           EPI   GL+G++KRL E  +    W PYGGMM KI E+ IPFPHR G LFKI Y   W +
Sbjct: 391 EPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFKILYYANWLE 450

Query: 447 GDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINK-KGNTSYIDAIGWGTRY 506
            D    + + WIK++YNYM PYVS  PR+AYVNYRDLD G NK     ++I+A  WG +Y
Sbjct: 451 NDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKY 510

Query: 507 FKENFGRLLRVKTKVDPDNFFRHEQSIPPIP 532
           FK NF RL+++KTKVDP+NFFRHEQSIPP+P
Sbjct: 511 FKGNFDRLVKIKTKVDPENFFRHEQSIPPMP 540

BLAST of MC01g_new0485 vs. NCBI nr
Match: XP_022146593.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 1105 bits (2857), Expect = 0.0
Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MVFPKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFN 60
           MVFPKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFN
Sbjct: 1   MVFPKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFN 60

Query: 61  ALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSY 120
           ALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSY
Sbjct: 61  ALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSY 120

Query: 121 ASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSL 180
           ASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSL
Sbjct: 121 ASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSL 180

Query: 181 GVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFG 240
           GVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFG
Sbjct: 181 GVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFG 240

Query: 241 IILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANAD 300
           IILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANAD
Sbjct: 241 IILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANAD 300

Query: 301 KKKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPE 360
           KKKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPE
Sbjct: 301 KKKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPE 360

Query: 361 VLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETE 420
           VLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETE
Sbjct: 361 VLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETE 420

Query: 421 IPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDL 480
           IPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDL
Sbjct: 421 IPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDL 480

Query: 481 GINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG 540
           GINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG
Sbjct: 481 GINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG 540

Query: 541 SSSREFD 547
           SSSREFD
Sbjct: 541 SSSREFD 547

BLAST of MC01g_new0485 vs. NCBI nr
Match: XP_038882764.1 (berberine bridge enzyme-like 15 [Benincasa hispida])

HSP 1 Score: 1027 bits (2656), Expect = 0.0
Identity = 505/546 (92.49%), Postives = 523/546 (95.79%), Query Frame = 0

Query: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63
           PKYKS V SLFLVFLLSSS WVDSASLEESFLQCLNENSQFSVP+SSFCAPNNATFNALL
Sbjct: 7   PKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNATFNALL 66

Query: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123
           QSTAQNLRYLE SVPKPLFIFTP +DSHVQ+AVICSKQLKIHLRVRSGGHDYEGLSY SE
Sbjct: 67  QSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSE 126

Query: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183
            ET F+ILDLAKLRS+KVDI+GNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG
Sbjct: 127 IETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 186

Query: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRIL+REAMGED+FWAIRGGGGGSFGIIL
Sbjct: 187 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIIL 246

Query: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKK- 303
           WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKK 
Sbjct: 247 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKG 306

Query: 304 -KTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEV 363
            KTVSTAYNALFLGDA+RLLKVMGESFPELGLTRKDCIE SWIKSVLYIAG+PSGTPPEV
Sbjct: 307 QKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEV 366

Query: 364 LLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEI 423
           LLQGKSTFKNYFKAKSDFVK+PI ETGLEGLWKRLFE+ESPL IWNPYGGMMGKISETEI
Sbjct: 367 LLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEI 426

Query: 424 PFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLG 483
           PFPHRRG+LFKIQYLT WQKG+ NQ+KHL+WIKKLYNYMTPYVSQLPRE YVNYRDLDLG
Sbjct: 427 PFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDLG 486

Query: 484 INKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFGS 543
           INK  NTSYI++IGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTS S RSFGS
Sbjct: 487 INKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESLRSFGS 546

Query: 544 SSREFD 547
           S R FD
Sbjct: 547 SKRGFD 552

BLAST of MC01g_new0485 vs. NCBI nr
Match: KAA0052471.1 (flavin-dependent oxidoreductase FOX2-like [Cucumis melo var. makuwa] >TYK13351.1 flavin-dependent oxidoreductase FOX2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 495/544 (90.99%), Postives = 519/544 (95.40%), Query Frame = 0

Query: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63
           PKYKS V  LFLVFLLSSS WVDS+SLEESFLQCLNENSQFSVP+SSFCAPNN+TFNALL
Sbjct: 7   PKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNSTFNALL 66

Query: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123
           QSTAQNLRYLE SVPKPLFIFTP +DSHVQ+AVICSKQL+IHLRVRSGGHDYEGLSYASE
Sbjct: 67  QSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGLSYASE 126

Query: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183
            ET F+ILDLAKLRS+KVDI+ NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG
Sbjct: 127 METPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 186

Query: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRIL+REAMGED+FWAIRGGGGGSFGIIL
Sbjct: 187 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIIL 246

Query: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKK 303
           WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN  +K 
Sbjct: 247 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANGGQK- 306

Query: 304 TVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLL 363
           TVSTAYNALFLGDA+RLLKVMGE+FPELGLTRKDCIE SWIKSVLYIAG+PSGTPPEVLL
Sbjct: 307 TVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 366

Query: 364 QGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPF 423
           QGKSTFKNYFKAKSDFVK PI ETGLEGLWKRLFE+ESPL IWNPYGGMMGKISETEIPF
Sbjct: 367 QGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPF 426

Query: 424 PHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 483
           PHRRG+LFKIQYLT WQKG+ NQ+KHL+WIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN
Sbjct: 427 PHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 486

Query: 484 KKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFGSSS 543
           K  NTSYI++IGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQS+PPIPTS + RSFGSS 
Sbjct: 487 KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSVPPIPTSEALRSFGSSK 546

Query: 544 REFD 547
           R FD
Sbjct: 547 RGFD 549

BLAST of MC01g_new0485 vs. NCBI nr
Match: XP_008439530.1 (PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo])

HSP 1 Score: 1011 bits (2613), Expect = 0.0
Identity = 496/544 (91.18%), Postives = 518/544 (95.22%), Query Frame = 0

Query: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63
           PKYKS V  LFLVFLLSSS WVDS SLEESFLQCLNENSQFSVP+SSFCAPNN+TFNALL
Sbjct: 7   PKYKSFVAPLFLVFLLSSSSWVDSFSLEESFLQCLNENSQFSVPYSSFCAPNNSTFNALL 66

Query: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123
           QSTAQNLRYLE SVPKPLFIFTP +DSHVQ+AVICSKQL+IHLRVRSGGHDYEGLSYASE
Sbjct: 67  QSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGLSYASE 126

Query: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183
            ET F+ILDLAKLRS+KVDI+ NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG
Sbjct: 127 METPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 186

Query: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRIL+REAMGED+FWAIRGGGGGSFGIIL
Sbjct: 187 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIIL 246

Query: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKK 303
           WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN  +K 
Sbjct: 247 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANGGQK- 306

Query: 304 TVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLL 363
           TVSTAYNALFLGDA+RLLKVMGE+FPELGLTRKDCIE SWIKSVLYIAG+PSGTPPEVLL
Sbjct: 307 TVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 366

Query: 364 QGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPF 423
           QGKSTFKNYFKAKSDFVK PI ETGLEGLWKRLFE+ESPL IWNPYGGMMGKISETEIPF
Sbjct: 367 QGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPF 426

Query: 424 PHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 483
           PHRRG+LFKIQYLT WQKG+ NQ+KHL+WIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN
Sbjct: 427 PHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 486

Query: 484 KKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFGSSS 543
           K  NTSYI++IGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTS + RSFGSS 
Sbjct: 487 KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEALRSFGSSK 546

Query: 544 REFD 547
           R FD
Sbjct: 547 RGFD 549

BLAST of MC01g_new0485 vs. NCBI nr
Match: XP_004134554.1 (berberine bridge enzyme-like 15 [Cucumis sativus] >KAE8647767.1 hypothetical protein Csa_003323 [Cucumis sativus])

HSP 1 Score: 1002 bits (2591), Expect = 0.0
Identity = 494/544 (90.81%), Postives = 513/544 (94.30%), Query Frame = 0

Query: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63
           PKYKS V  LFLVFLLSSS WVDS+SLEESFLQCLNENSQFSVP+SSFCAPNNATFNALL
Sbjct: 7   PKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNATFNALL 66

Query: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123
           QSTAQNLRYLE SVPKPLFIFTP  DSHVQ+AVICSKQL+IHLRVRSGGHDYEGLSYASE
Sbjct: 67  QSTAQNLRYLEPSVPKPLFIFTPLVDSHVQSAVICSKQLRIHLRVRSGGHDYEGLSYASE 126

Query: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183
            ET F+ILDLAKLRSIKVDIE NSAW QAGATIGEVYYRIAEKSKVHGFPAGLC SLGVG
Sbjct: 127 METPFIILDLAKLRSIKVDIESNSAWAQAGATIGEVYYRIAEKSKVHGFPAGLCPSLGVG 186

Query: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRIL+REAMGED+FWAIRGGGGGSFGIIL
Sbjct: 187 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIIL 246

Query: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKK 303
           WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLD+DLFIRVIINVAAN  +K 
Sbjct: 247 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDDDLFIRVIINVAANGGQK- 306

Query: 304 TVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLL 363
           TVSTAYNALFLGDA+RLLKVMGESFPEL LTRKDCIE SWIKSVLYIAG+PSGTPPEVLL
Sbjct: 307 TVSTAYNALFLGDASRLLKVMGESFPELSLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 366

Query: 364 QGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPF 423
           QGKSTFKNYFKAKSDFVK PI ETGLEGLWKRLFE+E PL IWNPYGGMMGKISETEIPF
Sbjct: 367 QGKSTFKNYFKAKSDFVKNPIPETGLEGLWKRLFEDEGPLMIWNPYGGMMGKISETEIPF 426

Query: 424 PHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 483
           PHRRG+LFKIQYLT WQK + NQ+KHL+WIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN
Sbjct: 427 PHRRGVLFKIQYLTTWQKVEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 486

Query: 484 KKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFGSSS 543
           K  NTSYI++IGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTS + RSFGSS 
Sbjct: 487 KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEALRSFGSSK 546

Query: 544 REFD 547
           R FD
Sbjct: 547 RGFD 549

BLAST of MC01g_new0485 vs. ExPASy TrEMBL
Match: A0A6J1CYZ1 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111015758 PE=3 SV=1)

HSP 1 Score: 1105 bits (2857), Expect = 0.0
Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MVFPKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFN 60
           MVFPKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFN
Sbjct: 1   MVFPKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFN 60

Query: 61  ALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSY 120
           ALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSY
Sbjct: 61  ALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSY 120

Query: 121 ASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSL 180
           ASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSL
Sbjct: 121 ASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSL 180

Query: 181 GVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFG 240
           GVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFG
Sbjct: 181 GVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFG 240

Query: 241 IILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANAD 300
           IILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANAD
Sbjct: 241 IILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANAD 300

Query: 301 KKKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPE 360
           KKKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPE
Sbjct: 301 KKKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPE 360

Query: 361 VLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETE 420
           VLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETE
Sbjct: 361 VLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETE 420

Query: 421 IPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDL 480
           IPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDL
Sbjct: 421 IPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDL 480

Query: 481 GINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG 540
           GINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG
Sbjct: 481 GINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG 540

Query: 541 SSSREFD 547
           SSSREFD
Sbjct: 541 SSSREFD 547

BLAST of MC01g_new0485 vs. ExPASy TrEMBL
Match: A0A5A7UG10 (Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009760 PE=3 SV=1)

HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 495/544 (90.99%), Postives = 519/544 (95.40%), Query Frame = 0

Query: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63
           PKYKS V  LFLVFLLSSS WVDS+SLEESFLQCLNENSQFSVP+SSFCAPNN+TFNALL
Sbjct: 7   PKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNSTFNALL 66

Query: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123
           QSTAQNLRYLE SVPKPLFIFTP +DSHVQ+AVICSKQL+IHLRVRSGGHDYEGLSYASE
Sbjct: 67  QSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGLSYASE 126

Query: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183
            ET F+ILDLAKLRS+KVDI+ NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG
Sbjct: 127 METPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 186

Query: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRIL+REAMGED+FWAIRGGGGGSFGIIL
Sbjct: 187 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIIL 246

Query: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKK 303
           WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN  +K 
Sbjct: 247 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANGGQK- 306

Query: 304 TVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLL 363
           TVSTAYNALFLGDA+RLLKVMGE+FPELGLTRKDCIE SWIKSVLYIAG+PSGTPPEVLL
Sbjct: 307 TVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 366

Query: 364 QGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPF 423
           QGKSTFKNYFKAKSDFVK PI ETGLEGLWKRLFE+ESPL IWNPYGGMMGKISETEIPF
Sbjct: 367 QGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPF 426

Query: 424 PHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 483
           PHRRG+LFKIQYLT WQKG+ NQ+KHL+WIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN
Sbjct: 427 PHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 486

Query: 484 KKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFGSSS 543
           K  NTSYI++IGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQS+PPIPTS + RSFGSS 
Sbjct: 487 KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSVPPIPTSEALRSFGSSK 546

Query: 544 REFD 547
           R FD
Sbjct: 547 RGFD 549

BLAST of MC01g_new0485 vs. ExPASy TrEMBL
Match: A0A1S3AYY2 (flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC103484298 PE=3 SV=1)

HSP 1 Score: 1011 bits (2613), Expect = 0.0
Identity = 496/544 (91.18%), Postives = 518/544 (95.22%), Query Frame = 0

Query: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63
           PKYKS V  LFLVFLLSSS WVDS SLEESFLQCLNENSQFSVP+SSFCAPNN+TFNALL
Sbjct: 7   PKYKSFVAPLFLVFLLSSSSWVDSFSLEESFLQCLNENSQFSVPYSSFCAPNNSTFNALL 66

Query: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123
           QSTAQNLRYLE SVPKPLFIFTP +DSHVQ+AVICSKQL+IHLRVRSGGHDYEGLSYASE
Sbjct: 67  QSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGLSYASE 126

Query: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183
            ET F+ILDLAKLRS+KVDI+ NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG
Sbjct: 127 METPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 186

Query: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRIL+REAMGED+FWAIRGGGGGSFGIIL
Sbjct: 187 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIIL 246

Query: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKK 303
           WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN  +K 
Sbjct: 247 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANGGQK- 306

Query: 304 TVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLL 363
           TVSTAYNALFLGDA+RLLKVMGE+FPELGLTRKDCIE SWIKSVLYIAG+PSGTPPEVLL
Sbjct: 307 TVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLL 366

Query: 364 QGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPF 423
           QGKSTFKNYFKAKSDFVK PI ETGLEGLWKRLFE+ESPL IWNPYGGMMGKISETEIPF
Sbjct: 367 QGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPF 426

Query: 424 PHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 483
           PHRRG+LFKIQYLT WQKG+ NQ+KHL+WIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN
Sbjct: 427 PHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYRDLDLGIN 486

Query: 484 KKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFGSSS 543
           K  NTSYI++IGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTS + RSFGSS 
Sbjct: 487 KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEALRSFGSSK 546

Query: 544 REFD 547
           R FD
Sbjct: 547 RGFD 549

BLAST of MC01g_new0485 vs. ExPASy TrEMBL
Match: A0A6J1IQ34 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111477806 PE=3 SV=1)

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 483/547 (88.30%), Postives = 513/547 (93.78%), Query Frame = 0

Query: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63
           PKYKS V SLFLVFLLSSS WV SASL+ESFLQCLNENSQFSVP+S+FCAPNNATFN LL
Sbjct: 8   PKYKSFVSSLFLVFLLSSSTWVASASLQESFLQCLNENSQFSVPYSTFCAPNNATFNTLL 67

Query: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123
           QS+AQNLRYLE SVPKPLFIFTP  DS+VQAAVICSKQLKIHLRVRSGGHDYEGLSY S+
Sbjct: 68  QSSAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQ 127

Query: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183
            E+ F+ILDLAKLRS+KVDI+ NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG
Sbjct: 128 IESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 187

Query: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRILDREAMGED+FWAIRGGGGGSFGIIL
Sbjct: 188 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIIL 247

Query: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKK- 303
           WWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN DKK 
Sbjct: 248 WWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKKG 307

Query: 304 -KTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEV 363
            KTVSTAYNA FLG+ANRLLK+MGESFPELGLTRKDCIE SWIKSVLYIAG+PSGTPPEV
Sbjct: 308 QKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEV 367

Query: 364 LLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEI 423
           LLQGKSTFKN+FKAKSDFV++PI ETGLEGLWKRL+EEESPL IWNPYGGMM KISET+I
Sbjct: 368 LLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQI 427

Query: 424 PFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLG 483
           PFPHRRG+L+KIQYLT WQK + N+++HL+WIKKLYNYM PYVSQLPREAYVNYRDLDLG
Sbjct: 428 PFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLG 487

Query: 484 INKKG-NTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG 543
           IN+K  NTSYI+AIGWGTRYF++NFGRLLRVKTKVDPDNFFRHEQSIPPIPTS S R FG
Sbjct: 488 INEKNSNTSYIEAIGWGTRYFRQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESLR-FG 547

Query: 544 SSSREFD 547
           SS R FD
Sbjct: 548 SSERGFD 553

BLAST of MC01g_new0485 vs. ExPASy TrEMBL
Match: A0A6J1ECW5 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111433029 PE=3 SV=1)

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 482/547 (88.12%), Postives = 512/547 (93.60%), Query Frame = 0

Query: 4   PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNATFNALL 63
           PKYKS V SLFLVFLLSSS WV SASL++SFLQCLNENSQFSVP+S+FCAPNNATFN LL
Sbjct: 8   PKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNNATFNTLL 67

Query: 64  QSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASE 123
           QSTAQNLRYLE SVPKPLFIFTP  DS+VQAAVICSKQLKIHLRVRSGGHDYEGLSY S+
Sbjct: 68  QSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSQ 127

Query: 124 TETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVG 183
            E+ F+ILDLAKLRS+KVDI+ NSAWVQAGATIGEVYYRI EKSKVHGFPAGLCTSLGVG
Sbjct: 128 IESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRITEKSKVHGFPAGLCTSLGVG 187

Query: 184 GHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIIL 243
           GHITGGAYG MMRKYGLGADNVVDARIVDANGRILDREAMGED+FWAIRGGGGGSFGIIL
Sbjct: 188 GHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGSFGIIL 247

Query: 244 WWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKK- 303
           WWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN DK+ 
Sbjct: 248 WWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAANTDKQG 307

Query: 304 -KTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEV 363
            KTVSTAYNA FLG+ANRLLK+MGESFPELGLTRKDCIE SWIKSVLYIAG+PSGTPPEV
Sbjct: 308 QKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEV 367

Query: 364 LLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEI 423
           LLQGKSTFKN+FKAKSDFV++PI ETGLEGLWKRL+EEESPL IWNPYGGMM KISET+I
Sbjct: 368 LLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKISETQI 427

Query: 424 PFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLG 483
           PFPHRRG+L+KIQYLT WQK + N+++HL+WIKKLYNYM PYVSQLPREAYVNYRDLDLG
Sbjct: 428 PFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYRDLDLG 487

Query: 484 INKKG-NTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASFRSFG 543
           IN+K  NTSYI+AIGWGTRYFK+NFGRLLRVKTKVDPDNFFRHEQSIPPIPTS S R FG
Sbjct: 488 INEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESLR-FG 547

Query: 544 SSSREFD 547
           SS R FD
Sbjct: 548 SSERGFD 553

BLAST of MC01g_new0485 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 734.6 bits (1895), Expect = 5.9e-212
Identity = 353/522 (67.62%), Postives = 434/522 (83.14%), Query Frame = 0

Query: 12  SLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPF-SSFCAPN-NAT-FNALLQSTAQ 71
           +LFLV LL  S  + S++L++ F++CL +NS  S P  +SF +P+ NAT F   L+STAQ
Sbjct: 11  TLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQ 70

Query: 72  NLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASETETSF 131
           NLRYL  S PKP+FIF P Y++HVQAAV+C+K+L++HLR+RSGGHDYEGLS+ +E ET F
Sbjct: 71  NLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPF 130

Query: 132 MILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITG 191
           +I+DL+KLR + VD++ NSAW  AGATIGEVYYRI EKS+ HGFPAGLC+SLG+GGH+ G
Sbjct: 131 VIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVG 190

Query: 192 GAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILWWKLN 251
           GAYG MMRK+GLGADNV+DARIVDANG+ILDR AMGEDVFWAIRGGGGGSFG+IL WK+ 
Sbjct: 191 GAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIK 250

Query: 252 LVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADK--KKTVS 311
           LVPVP+TVTVFTV KTLEQ  TK+LYKW+Q+ADKLD+DLFIRVII+ A+   K   +T+S
Sbjct: 251 LVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTIS 310

Query: 312 TAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLLQGK 371
            +Y A FLGD+NRLL+VM +SFPELGLT+KDC EMSWIKSV+YIAGFP+   PE LL GK
Sbjct: 311 MSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGK 370

Query: 372 STFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPFPHR 431
           S FKN+FKAKSDFVKEPI   GLEGLW+R  EE+SPLTIWNPYGGMM +ISE+EIPFPHR
Sbjct: 371 SLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHR 430

Query: 432 RGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKKG 491
            G LFKIQ+L+ WQ G  ++E+H++WI+++Y+YM  YVS+ PR+AYVNYRDLDLG N +G
Sbjct: 431 NGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTN-EG 490

Query: 492 NTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIP 529
            T   DA  WG +Y+K NF RL+++K + DPDNFFRHEQS+P
Sbjct: 491 ET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of MC01g_new0485 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 729.9 bits (1883), Expect = 1.5e-210
Identity = 354/519 (68.21%), Postives = 420/519 (80.92%), Query Frame = 0

Query: 15  LVFLLSSSCWVDSASLEESFLQCLNENSQFSVPF-SSFCAP--NNATFNALLQSTAQNLR 74
           L+ L  S   +  +++++ F+ CL +NS  S P  SSF     N ++F   L+++AQNLR
Sbjct: 16  LLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLR 75

Query: 75  YLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASETETSFMIL 134
           YL  S PKP FIF P Y++HVQAAV+C+K+LK+HLR+RSGGHDYEGLSY SE ET+F+I+
Sbjct: 76  YLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIV 135

Query: 135 DLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAY 194
           DL+KLR I VDIE NSAWV AGA+IGEVYYRI EKSK+HGFPAGLCTSLG+GGHI GGAY
Sbjct: 136 DLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAY 195

Query: 195 GPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILWWKLNLVP 254
           G MMRK+GLGADNV+DARIVDA+G+IL+R AMGEDVFWAIRGGGGGSFG+IL WK+ LVP
Sbjct: 196 GSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVP 255

Query: 255 VPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKK--TVSTAY 314
           VP  VTVFTV +TLEQ  TK+LYKWQQVADKLDEDLFIRVII   +   K K  T+ST+Y
Sbjct: 256 VPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSY 315

Query: 315 NALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLLQGKSTF 374
              FLGDANRLL+VM  SFP+LGLT+KDC+E SWIKSV+YIAGFPS  P E LL GKS F
Sbjct: 316 QGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLF 375

Query: 375 KNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPFPHRRGI 434
           KNYFKAKSD+V+EPI   GLEGLW++L EE+SPLTIWNPYGGMM KI ETE PFPHR G 
Sbjct: 376 KNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFPHRSGT 435

Query: 435 LFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKKGNTS 494
           LFKIQ+LTLWQ G +++ KH+ W++++Y+YM  YVS+ PR AYVNYRDLDLG+N KG+  
Sbjct: 436 LFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKGS-- 495

Query: 495 YIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIP 529
             DA  WG RYFK NF RL+ +K K DP+NFFRHEQSIP
Sbjct: 496 --DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of MC01g_new0485 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 696.8 bits (1797), Expect = 1.4e-200
Identity = 333/509 (65.42%), Postives = 410/509 (80.55%), Query Frame = 0

Query: 28  ASLEESFLQCLNENSQFSVPF-SSFCAP--NNATFNALLQSTAQNLRYLETSVPKPLFIF 87
           ASL++ F+ C+  N+    P   +F AP  N + F+ +L+STAQNLR+L+ S+PKP FIF
Sbjct: 29  ASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIF 88

Query: 88  TPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASETETSFMILDLAKLRSIKVDIE 147
           +P ++SHVQA++ICSK+L++HLRVRSGGHDYEGLSY S+ +  F+++DL+K+R + ++I+
Sbjct: 89  SPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQ 148

Query: 148 GNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAYGPMMRKYGLGADN 207
            NSAWVQ+GAT+GE+YYRIAEKSKVHGFPAGLC+SLG+GGHITGGAYG MMRKYGLGADN
Sbjct: 149 DNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADN 208

Query: 208 VVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILWWKLNLVPVPSTVTVFTVAKT 267
           V+DA+IVDANG++LDR AMGED FWAIRGG GGSFGIIL WK+ LVPVP TVTVFTV KT
Sbjct: 209 VLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKT 268

Query: 268 LEQG-ATKILYKWQQVADKLDEDLFIRVIINVAANADKKKTVSTAYNALFLGDANRLLKV 327
           L+Q    KI+ KWQ+VADKL E+LFIRV+ NVA      KTV+T+YNALFLG    L+ V
Sbjct: 269 LQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGG-NKTVTTSYNALFLGGKGTLMNV 328

Query: 328 MGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLLQGKSTF-KNYFKAKSDFVKE 387
           M +SFPELGLT KDCIEMSW++S+ YI+GFP+ TP  VLLQGKS F K  FKAKSDFVK 
Sbjct: 329 MKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKT 388

Query: 388 PISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPFPHRRGILFKIQYLTLWQKG 447
           PI E+GL+G++K+L +E+ PL IWNPYGGMM KI E++IPFPHR+G+LFK+QY+T W   
Sbjct: 389 PIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDS 448

Query: 448 DSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKKG-NTSYIDAIGWGTRYF 507
           D    +H+ WI+ LY+YMTPYVS  PREAYVNYRDLDLG N K   T    A  WG  YF
Sbjct: 449 DKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYF 508

Query: 508 KENFGRLLRVKTKVDPDNFFRHEQSIPPI 531
           K NF RL+ +K KVDP+NFFRHEQSIPP+
Sbjct: 509 KNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of MC01g_new0485 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 648.7 bits (1672), Expect = 4.3e-186
Identity = 319/532 (59.96%), Postives = 399/532 (75.00%), Query Frame = 0

Query: 8   SVVGSLFLVFLLSSSCWVDSASLEESFLQCL--NENSQFSVPFSSFCAPNNAT-FNALLQ 67
           S +    L F   +     S SL++ F++CL  N N +F++  + F    NA+ F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 68  STAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASET 127
           STAQN RYL  ++PKP FIF P ++SHVQA+VICSK+L+IH RVRSGGHDYEG+SY S+ 
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 128 ETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 187
           E  F+++DL+KLR I VDI+  SAWV+AGAT+GE+YYRIAEKSK HGFPAG+  SLG+GG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 188 HITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILW 247
           HITGGAYG +MRKYGL ADNV+DA+IVDANG++LDR +MGED+FWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 248 WKLNLVPVPSTVTVFTVAKTLEQGAT-KILYKWQQVADKLDEDLFIRVIINVAANADKKK 307
           WK+ LVPVP T+TVFTV KT EQ  + KIL KWQ++AD L ++LF+RV   V+ N    K
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN-KANK 310

Query: 308 TVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPP--EV 367
           TV+ AY   FLG+   L++VM + FPELGLT+KDCIEMSWI S++Y +GFP+  PP  E+
Sbjct: 311 TVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEI 370

Query: 368 LLQGKSTF-KNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETE 427
           LLQ KS   K YFK KSDF K+PI   GLEG++K+L EE++ L IW PYGG M KI E+E
Sbjct: 371 LLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESE 430

Query: 428 IPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDL 487
           IPFPHR G  F IQY   W   +    +  +WI++LY YMTPYVS  PR+AYVNYRDLDL
Sbjct: 431 IPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDL 490

Query: 488 GINKKGNTS-YIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIP 532
           G NK  + S +I+A  WG  YFK+NF RL+R+K+KVDPDNFFRHEQSIP +P
Sbjct: 491 GQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLP 541

BLAST of MC01g_new0485 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 645.6 bits (1664), Expect = 3.6e-185
Identity = 309/511 (60.47%), Postives = 386/511 (75.54%), Query Frame = 0

Query: 27  SASLEESFLQCLNENSQFSVPFSSFC---APNNATFNALLQSTAQNLRYLETSVPKPLFI 86
           SAS+++ F+ C+  N+  S P        A N + FN +L+STAQNL++L  S+PKP FI
Sbjct: 31  SASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKPGFI 90

Query: 87  FTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYASETETSFMILDLAKLRSIKVDI 146
           F P + S VQA++ICSK+L IH RVRSGGHD+E LSY S  E  F++LDL+KL+ I VDI
Sbjct: 91  FRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQINVDI 150

Query: 147 EGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAYGPMMRKYGLGAD 206
           E NSAWVQ GAT+GE+YYRIAEKSK+HGFPAGLCTS+G+GG++TGG YG +MRKYGL  D
Sbjct: 151 ESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGLAGD 210

Query: 207 NVVDARIVDANGRILDREAMGEDVFWAIRGGGGGSFGIILWWKLNLVPVPSTVTVFTVAK 266
           NV+D ++VDANG++LDR AMGED+FWAIRGGGG SFGI+L WK+ LVPVP TVTVFTV K
Sbjct: 211 NVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFTVTK 270

Query: 267 TLEQGA-TKILYKWQQVADKLDEDLFIRVIINVAANADKKKTVSTAYNALFLGDANRLLK 326
           TLEQ A  K + KWQQ++ K+ E++ IRV++  A N D  KTV+  Y   FLG+   LLK
Sbjct: 271 TLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN-DGNKTVTMTYLGQFLGEKGTLLK 330

Query: 327 VMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSGTPPEVLLQGKSTF-KNYFKAKSDFVK 386
           VM ++FPELGLT+KDC EMSWI++ L+  GFP+G+P E+LLQ KS   K+YFKA SDFVK
Sbjct: 331 VMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKSPLGKDYFKATSDFVK 390

Query: 387 EPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKISETEIPFPHRRGILFKIQYLTLWQK 446
           EPI   GL+G++KRL E  +    W PYGGMM KI E+ IPFPHR G LFKI Y   W +
Sbjct: 391 EPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFKILYYANWLE 450

Query: 447 GDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINK-KGNTSYIDAIGWGTRY 506
            D    + + WIK++YNYM PYVS  PR+AYVNYRDLD G NK     ++I+A  WG +Y
Sbjct: 451 NDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKY 510

Query: 507 FKENFGRLLRVKTKVDPDNFFRHEQSIPPIP 532
           FK NF RL+++KTKVDP+NFFRHEQSIPP+P
Sbjct: 511 FKGNFDRLVKIKTKVDPENFFRHEQSIPPMP 540