Homology
BLAST of MC01g_new0423 vs. ExPASy Swiss-Prot
Match:
O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)
HSP 1 Score: 510.0 bits (1312), Expect = 3.3e-143
Identity = 258/528 (48.86%), Postives = 354/528 (67.05%), Query Frame = 0
Query: 8 NWLSLITTLLAGVIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGPELPHIKL 67
N L+L T +A ++++ F L S+G P V+G WP++GHL LL G + PH L
Sbjct: 6 NCLNLNTIAIASILSLIFLCLFLYR-KNSRGKDAPVVSGAWPILGHLSLLNGSQTPHKVL 65
Query: 68 SALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYAMFA 127
ALA++YGP+F+I+LG++ A+V+++WE +K+ FTT+D+A+SSRP ++A +++ YN A
Sbjct: 66 GALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVG 125
Query: 128 FGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKE-RRDEQGQVLV 187
PY YWR++RK LSNRR+E ++R SEV +IKEL+ W ++E LV
Sbjct: 126 LAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLV 185
Query: 188 EMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTLGDVVPY 247
++KQW+ LT N+++ +V GKRYFG + + +A+ + +EF+ LMG FT+ D VP
Sbjct: 186 DIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPC 245
Query: 248 LGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKE-DKDFMDVMLSVLEEETA 307
L WLD+GGH KAMK +KE+D +L+ WLEEHR+ + G + E D+DFMDVM+S L
Sbjct: 246 LRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALN---- 305
Query: 308 NVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDARI 367
+FD DTI KAT L ++LGG+D+T V +TWALSLLL N AL K ++E+D +I
Sbjct: 306 ---GAQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQI 365
Query: 368 GRDRELNESDIGKLVYFQAVVKETMRLYGVVGL-FVRETSEDFAIDGFHIEKGTWLFMNL 427
G+D + ESDI KLVY QA+VKET+RLY RE +E+ + G+HI+KGT L NL
Sbjct: 366 GKDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNL 425
Query: 428 WKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQLI 487
WKIHRD VW +PLEFKPERFLTTHKD D++G FEL+PFG GRR C+GMSLGL MV
Sbjct: 426 WKIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFT 485
Query: 488 LGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALYD 533
L LL +FDI VDMT G +N KA+PLE+ +KPR S Y+
Sbjct: 486 LANLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPRQSPNYYE 525
BLAST of MC01g_new0423 vs. ExPASy Swiss-Prot
Match:
Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)
HSP 1 Score: 495.7 bits (1275), Expect = 6.4e-139
Identity = 254/548 (46.35%), Postives = 352/548 (64.23%), Query Frame = 0
Query: 8 NWLSLITTLLAGVIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGPELPHIKL 67
N+L+ T +I++ FF F + +K +PP ++G WPL+GHLPL+ + PH L
Sbjct: 4 NYLNTTTIAFISLISLLFFLFRFSKVSHTK--EPPIISGSWPLLGHLPLMRNTQTPHKTL 63
Query: 68 SALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYAMFA 127
AL ++YGPIF+I+LG A+V+++WE AK+CFT +D+ +SSRP +A +++ YN A
Sbjct: 64 GALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIG 123
Query: 128 FGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQ------ 187
+ PY +YWR++RK L +LSNRR+ELL ++R SEV +IKEL W + Q
Sbjct: 124 WAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDD 183
Query: 188 -------------GQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRAT 247
V VE+K+W LT+N++L +V GKR FG + + EA+
Sbjct: 184 TKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENI 243
Query: 248 KEFLRLMGLFTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKE 307
++F+RL+G FT+GD VP+L WLD+GGH K MK+ +K+ D ML WLEEHR+ + G +
Sbjct: 244 RDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDK 303
Query: 308 ---DKDFMDVMLSVLEEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALS 367
++DFMD ML VL+++ FD DTIIKAT L ++LGGSDTT +TWA+
Sbjct: 304 VVGERDFMDAMLLVLKDK-------PIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMC 363
Query: 368 LLLNNRPALRKVQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGL-FVRETS 427
LLL + L K+++EL+ IG++R +NESDI KLVY A++KET+RLY RE +
Sbjct: 364 LLLKHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFT 423
Query: 428 EDFAIDGFHIEKGTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPF 487
ED I G+HI+KGT L NLWKIHRD VW +PLEFKPERFL+THKD DV+GQ FEL+PF
Sbjct: 424 EDCTIGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPF 483
Query: 488 GGGRRACSGMSLGLQMVQLILGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKP 533
G GRR C+GMSLGL MV IL L +F+I ++D+T + KA+PLEV +KP
Sbjct: 484 GSGRRMCAGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKP 542
BLAST of MC01g_new0423 vs. ExPASy Swiss-Prot
Match:
O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)
HSP 1 Score: 489.6 bits (1259), Expect = 4.6e-137
Identity = 249/530 (46.98%), Postives = 350/530 (66.04%), Query Frame = 0
Query: 10 LSLITTLLAGVIAINFFRFLL------KTFTASKGLKPPTVAGGWPLIGHLPLLAGPELP 69
L + T GV+++ F L K +SK + PTV G WP+ GHLPLL G + P
Sbjct: 5 LHFLNTTTIGVVSLIFLLCLFLYGPLKKVHGSSK--EAPTVGGAWPIFGHLPLLIGSKSP 64
Query: 70 HIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNY 129
H L ALA ++GP+F+I+LG +KA+V++ WE A++CFTT+DVA+S+RP +L ++++ YN
Sbjct: 65 HKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNN 124
Query: 130 AMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQ 189
AM PY YWR++RK +LS+ R+E L++VR SEV +I ELY W+ +++E
Sbjct: 125 AMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDY 184
Query: 190 VLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTLGDV 249
VE+KQW N++L +V GKR+ A A + +A C +A EF+RL G+FT+GD
Sbjct: 185 ASVELKQWFAQPIFNMVLRMVVGKRFLSATA--TDEKAEKCVKAVDEFMRLAGVFTVGDA 244
Query: 250 VPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKED-KDFMDVMLSVLEE 309
+PYL WLD GG+ KAMK T+KELD M++ WLEEHR+ RA G + +DFM+VMLS L+
Sbjct: 245 IPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSLDG 304
Query: 310 ETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELD 369
+T + D DT+IK+T LT++ G++ ++ + WA+ L+L N P + + + ELD
Sbjct: 305 KTID-------GIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKN-PLILENKAELD 364
Query: 370 ARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGL-FVRETSEDFAIDGFHIEKGTWLF 429
++G+DR + ESDI LVY QAVVKET+RLY L RE +ED + G+H++KGT L
Sbjct: 365 IQVGKDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLI 424
Query: 430 MNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGLQMV 489
N+WKIH D VW +P EFKP+RFLTTHKD DVKG F+L+PFG GRR C G+S GLQ V
Sbjct: 425 TNIWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTV 484
Query: 490 QLILGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALY 532
L L L +F+I +DMT + G++N KA+PLEV +KP LS + Y
Sbjct: 485 HLALASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCY 522
BLAST of MC01g_new0423 vs. ExPASy Swiss-Prot
Match:
A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)
HSP 1 Score: 481.9 bits (1239), Expect = 9.5e-135
Identity = 260/524 (49.62%), Postives = 347/524 (66.22%), Query Frame = 0
Query: 10 LSLITTLLAG--VIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGPELPHIKL 69
L + TLL G V+ FF F+ K +K +PP AG WP+IGHL LLA +LPH L
Sbjct: 4 LHCLETLLLGFFVLLPCFFYFVWKK-PNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKIL 63
Query: 70 SALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYAMFA 129
S+ A++ GP+F IQLG+ +A+V+N+ E AK+CFTT+D +RP+ +A+KI+GYNY M
Sbjct: 64 SSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLG 123
Query: 130 FGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQVLVE 189
PY YWR MRK L LSNRRL+ L++V SE++ + KELY W+ + + LV+
Sbjct: 124 VAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNID--FCLVD 183
Query: 190 MKQWVGDLTMNLILGVVAGKRYFGAAAMVA--EAEARACHRATKEFLRLMGLFTLGDVVP 249
MKQW+ DLT+N+ + +V GKR+FG+A+ A E E+ C + + RLMG F L D +P
Sbjct: 184 MKQWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLP 243
Query: 250 YLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRAS-GRSKEDKDFMDVMLSVLEEET 309
YL WLD+GGH K MKRT KELD + WL+EH++ R S G+ +D+DFMDVMLS+LEE
Sbjct: 244 YLRWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEE-- 303
Query: 310 ANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDAR 369
S D DTI K CL ++LGG+DTT +TWALSLLLNN AL+K Q ELD
Sbjct: 304 ------SKLGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLH 363
Query: 370 IGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLF-VRETSEDFAIDGFHIEKGTWLFMN 429
+GRDR ++ESD+ KL Y A++KET+RLY L R +ED I G+H+ GT L +N
Sbjct: 364 VGRDRNVDESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVN 423
Query: 430 LWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQL 489
WKI RD VW +P E++PERFL +D D+KGQ FELIPFG GRRAC +SL LQ++ L
Sbjct: 424 AWKIQRDPLVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPL 483
Query: 490 ILGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLS 528
L +L F++ T VDMT + G+ + KA+PLEV + PR+S
Sbjct: 484 TLAHILHGFELRTPNQNKVDMTETPGIVHAKATPLEVLVAPRIS 514
BLAST of MC01g_new0423 vs. ExPASy Swiss-Prot
Match:
O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)
HSP 1 Score: 476.1 bits (1224), Expect = 5.2e-133
Identity = 248/526 (47.15%), Postives = 352/526 (66.92%), Query Frame = 0
Query: 14 TTLLAGVIAINFFRF--LLKTFTASKGLKPPTVAGGWPLIGHLPLLAGPE-LPHIKLSAL 73
T+L + + I F F L K K +K P +G WP+IGHL LL+G E L + L +
Sbjct: 3 TSLFSLFVPILVFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKM 62
Query: 74 ANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYAMFAFGP 133
A++YGP S++LG + V++S+E AK CFT +D A++SRP A+K +GY+ A+F F P
Sbjct: 63 ADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAP 122
Query: 134 YDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQVLVEMKQ 193
Y ++WR+MRK L LLSNRRL++L++VR SE++ +++LY+ W ++ + V+V++K
Sbjct: 123 YSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSE-PVMVDLKS 182
Query: 194 WVGDLTMNLILGVVAGKRYFGAAAMVAE--AEARACHRATKEFLRLMGLFTLGDVVPYLG 253
W+ D+++N+++ +VAGKRYFG ++ E EAR C + F L+G+FT+ D P LG
Sbjct: 183 WLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLG 242
Query: 254 WLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDR-ASGRSKEDKDFMDVMLSVLEEETANV 313
W D GH K MK+T +ELD +L RW+E HR+ R SG D DF+DVMLS+ E+
Sbjct: 243 WFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQ---GK 302
Query: 314 VSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDARIGR 373
S H D T IK+TCL ++LGGS+T+ +TWA+SLLLNN+ L+K Q E+D +GR
Sbjct: 303 FSHLQH--DAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGR 362
Query: 374 DRELNESDIGKLVYFQAVVKETMRLYGVVGLF-VRETSEDFAIDGFHIEKGTWLFMNLWK 433
DR + +SDI LVY QA++KET+RLY L RE ED + G+++ +GT + +N+WK
Sbjct: 363 DRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWK 422
Query: 434 IHRDERVWGEPLEFKPERFLTTH-KDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQLIL 493
I RD RV+ EP EF+PERF+T K+FDV+GQ FEL+PFG GRR+C G SL +Q++ L L
Sbjct: 423 IQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGL 482
Query: 494 GGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALY 532
LQ+FD+ TV D VDMT S GL+ KA+PLE+ I PRL LY
Sbjct: 483 ARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522
BLAST of MC01g_new0423 vs. NCBI nr
Match:
XP_022132489.1 (cytochrome P450 CYP82D47-like [Momordica charantia] >XP_022132490.1 cytochrome P450 CYP82D47-like [Momordica charantia])
HSP 1 Score: 930 bits (2403), Expect = 0.0
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0
Query: 1 MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGP 60
MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGP
Sbjct: 1 MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGP 60
Query: 61 ELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILG 120
ELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILG
Sbjct: 61 ELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILG 120
Query: 121 YNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDE 180
YNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDE
Sbjct: 121 YNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDE 180
Query: 181 QGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTL 240
QGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTL
Sbjct: 181 QGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTL 240
Query: 241 GDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVL 300
GDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVL
Sbjct: 241 GDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVL 300
Query: 301 EEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQE 360
EEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQE
Sbjct: 301 EEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQE 360
Query: 361 LDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEKGTWL 420
LDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEKGTWL
Sbjct: 361 LDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEKGTWL 420
Query: 421 FMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFG 465
FMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFG
Sbjct: 421 FMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFG 465
BLAST of MC01g_new0423 vs. NCBI nr
Match:
XP_022133138.1 (cytochrome P450 CYP82D47-like [Momordica charantia])
HSP 1 Score: 834 bits (2154), Expect = 4.74e-301
Identity = 420/500 (84.00%), Postives = 449/500 (89.80%), Query Frame = 0
Query: 1 MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFTA---SKGLKPPTVAGGWPLIGHLPLL 60
MEP+ QF+WL+LI TLL GVIAINF RFL KTFTA SKGLKPP+V GGWPLIGHLPLL
Sbjct: 1 MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL 60
Query: 61 AGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASK 120
GP+LPH+KL ALA+EYGPIFSIQLGI +AVVINSWEAAKQCFTTHDVA+S RP ILA++
Sbjct: 61 GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ 120
Query: 121 ILGYNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKER 180
ILGYNYA+FAFGPYDSYWRKMRKTT LHLLS+RRLELLR+VRESEVNQAIKELY WKER
Sbjct: 121 ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER 180
Query: 181 RDEQGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGL 240
RD+QGQ LVEMKQWVGDLTMNLILGVVAGKRYFG AMV EAEARAC +A KEFLRLMGL
Sbjct: 181 RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL 240
Query: 241 FTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVML 300
F LGD VPYLGWLDVGGHVKAMKRTSKELDH+LT+WLEEH+K RASG+SKEDKDFMDVML
Sbjct: 241 FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML 300
Query: 301 SVLEEETANVV-SDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRK 360
S LEE NVV SDS HSFD DTIIKATCLTMVLGGSDTT VVVTWALSLLLNN PAL K
Sbjct: 301 STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK 360
Query: 361 VQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEK 420
VQ+E+DA IGRDREL ESD+ +LVY QAVVKETMRLYG GL +RETSEDFAIDGFHIEK
Sbjct: 361 VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK 420
Query: 421 GTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSL 480
GT LF+N WKIHRD RVWGEPLEFKPERFLTTH+DFDV GQQFELIPFGGGRRAC G+S
Sbjct: 421 GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSF 480
Query: 481 GLQMVQLILGGLLQAFDIST 496
GLQMVQLILG LL AFDIS+
Sbjct: 481 GLQMVQLILGALLHAFDISS 500
BLAST of MC01g_new0423 vs. NCBI nr
Match:
XP_022133139.1 (cytochrome P450 CYP82D47-like [Momordica charantia])
HSP 1 Score: 698 bits (1801), Expect = 2.27e-249
Identity = 351/408 (86.03%), Postives = 373/408 (91.42%), Query Frame = 0
Query: 125 MFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQV 184
MF FGPY+SYWRKMRKTTN+HLLSNRRLELLRNVRESEV+QAIKELY WK+RRD QG+
Sbjct: 1 MFGFGPYNSYWRKMRKTTNVHLLSNRRLELLRNVRESEVSQAIKELYQIWKKRRDGQGRG 60
Query: 185 LVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTLGDVV 244
LVEMKQW+G+LTMNLILGVVA KRYFG AAMV E EARAC +ATKEFLRLMGLFTLGD
Sbjct: 61 LVEMKQWIGELTMNLILGVVASKRYFGPAAMVEEDEARACQKATKEFLRLMGLFTLGDAA 120
Query: 245 PYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVLEEET 304
P+LGWLDVGGHVKAMKRTSKELDH+LT+WLEEH+KDRASG SKEDKDFMDVMLS LEE
Sbjct: 121 PHLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKDRASGGSKEDKDFMDVMLSTLEETP 180
Query: 305 ANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDAR 364
VVSDS HSFD DTIIKATCLTMVLGG+DTTVVVVTW LSLLLNNRPALRKVQ+ELDA
Sbjct: 181 DAVVSDSIHSFDVDTIIKATCLTMVLGGNDTTVVVVTWTLSLLLNNRPALRKVQEELDAH 240
Query: 365 IGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEKGTWLFMNL 424
IGR+REL ESD+ +LVY QAVVKETMRLYG VGL +RETS DFAIDGF IEKGTW+F+NL
Sbjct: 241 IGRERELKESDMSELVYLQAVVKETMRLYGAVGLILRETSVDFAIDGFQIEKGTWIFINL 300
Query: 425 WKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQLI 484
WKIHRDERVWGEPLEFKPERFLTTH DFDVKGQQFELIPFGGGRRACSG+S GLQMVQLI
Sbjct: 301 WKIHRDERVWGEPLEFKPERFLTTHIDFDVKGQQFELIPFGGGRRACSGISFGLQMVQLI 360
Query: 485 LGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALYD 532
LG LL AFDISTVEDAAVDMTGSIGLSNMKA+PLEVFIKPRL ALYD
Sbjct: 361 LGTLLHAFDISTVEDAAVDMTGSIGLSNMKATPLEVFIKPRLPFALYD 408
BLAST of MC01g_new0423 vs. NCBI nr
Match:
XP_038883369.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])
HSP 1 Score: 694 bits (1791), Expect = 8.01e-246
Identity = 362/544 (66.54%), Postives = 421/544 (77.39%), Query Frame = 0
Query: 1 MEPNAQFNWLSLITTLLAGV---IAINFFRFLLKTFTASKG-------LKPPTVAGGWPL 60
+E A+ W + TT+L G+ +AI FR ++K T + G KPP V GGWPL
Sbjct: 3 VEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPL 62
Query: 61 IGHLPLLAGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSR 120
I HLPL GPE PH+KLSALA++YGPIFSIQLG AVVIN+WEAAKQCFTTHD+A+ R
Sbjct: 63 IRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPR 122
Query: 121 PNILASKILGYNYAMFAFGPY-DSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKE 180
P +L+ K++GYNYAMF F PY +SYWRKMRK T L LLSNRRL+LLR VRESEVN+AIKE
Sbjct: 123 PKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKE 182
Query: 181 LYTAWKERRDEQGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATK 240
LY Q VEMKQW GDL MNL+L +V GKRYFGA AMV E EA+ C +A +
Sbjct: 183 LY---------QLGGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAME 242
Query: 241 EFLRLMGLFTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKED 300
E LRL+G++ LGD +PYLGWLDVGGHVKAMKRT K LD MLT+WLEEH+K+RA K +
Sbjct: 243 ESLRLLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAG---KNN 302
Query: 301 KDFMDVMLSVLEEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLN 360
DFMDVMLSVLEE T N D H F DT+IKATCLTMVLG S+T++V V WALSLLLN
Sbjct: 303 GDFMDVMLSVLEE-TDN---DILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLN 362
Query: 361 NRPALRKVQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAI 420
NR LRKVQ+ELD IGRD +L ESD+ KLVY +AVVKE MRL+G L RE +ED +
Sbjct: 363 NRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDIDV 422
Query: 421 DGFHIEKGTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKG-QQFELIPFGGGR 480
DGFHI+KGTWLF+NLWKIHRDERVW EPL+FKPERFLTTHK+FDVKG QQFELIPFGGGR
Sbjct: 423 DGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGGR 482
Query: 481 RACSGMSLGLQMVQLILGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSH 532
R C G+ GLQ+V+ +LGGLL AFDI T EDAAVDMTG+IG++NMKA+PLEVFIKPRLS
Sbjct: 483 RICPGLWFGLQLVEFVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSP 530
BLAST of MC01g_new0423 vs. NCBI nr
Match:
KAG2663852.1 (hypothetical protein I3760_16G051800 [Carya illinoinensis])
HSP 1 Score: 611 bits (1575), Expect = 3.68e-213
Identity = 313/533 (58.72%), Postives = 389/533 (72.98%), Query Frame = 0
Query: 6 QFNWLSLITTLLAGVIAINFFR-FLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGP-ELP 65
FN SL T++AG+ +I F+ K F ASK KPP AGGWPLIGHL LL G +LP
Sbjct: 2 DFNLPSL-NTIIAGLFSIIILSYFIQKRFGASKSRKPPEAAGGWPLIGHLHLLGGKSQLP 61
Query: 66 HIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNY 125
HI L +LA++YG +FSI++G+ A+VI+SWE AK+CFTT+DVA++SRP + +K LGY+Y
Sbjct: 62 HIVLGSLADKYGSVFSIRIGVHSALVISSWELAKECFTTNDVALASRPKLAGAKYLGYDY 121
Query: 126 AMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQ-- 185
M F PY SYWR+MRK +L LLSNRRLELL++VR SE+ ++KELY W E++D
Sbjct: 122 VMIGFSPYGSYWREMRKIISLELLSNRRLELLKDVRASEMETSLKELYKVWTEKKDGSDG 181
Query: 186 --GQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFT 245
G V VEMKQW GDL +N++L +VAGKRYFGAAA A+ EAR C +A +EF LMG F
Sbjct: 182 GGGGVSVEMKQWFGDLALNVVLRMVAGKRYFGAAA-TADKEARRCQKAFREFFHLMGQFL 241
Query: 246 LGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSV 305
+GD +PYLGW+D+GGHVKAMK+T+KE+D M T WLEEHRK+R SG SK+ +DFMD+MLSV
Sbjct: 242 VGDAIPYLGWMDLGGHVKAMKKTAKEMDCMATEWLEEHRKNRVSGESKQQQDFMDIMLSV 301
Query: 306 LEEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQ 365
LE FD DT+ K+ CL M+ GG+DTT+V +TW LSLLLNNR AL K Q+
Sbjct: 302 LE-------GADLGDFDADTVNKSACLNMISGGTDTTMVSLTWTLSLLLNNRHALIKAQE 361
Query: 366 ELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLF-VRETSEDFAIDGFHIEKGT 425
ELD +GR R +NE DI KL Y QA+VKE +RLY L RE +ED I GFH+ KGT
Sbjct: 362 ELDMHVGRGRLVNEGDISKLFYLQAIVKEALRLYPPGPLSGPREAAEDCTIGGFHVAKGT 421
Query: 426 WLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGL 485
+ NLWKI D ++W EPLEFKPERFLTTHKD DV+GQ FELIPFG GRRAC G+S GL
Sbjct: 422 RVVFNLWKIQTDPKIWSEPLEFKPERFLTTHKDVDVRGQNFELIPFGSGRRACPGISFGL 481
Query: 486 QMVQLILGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALY 531
QM+ L L LL AF+IST +A VDM+ + GLSN+K +PL+V +KPRLS LY
Sbjct: 482 QMLHLTLASLLHAFEISTPSNATVDMSATFGLSNIKTTPLDVVLKPRLSPNLY 525
BLAST of MC01g_new0423 vs. ExPASy TrEMBL
Match:
A0A6J1BSL4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005334 PE=3 SV=1)
HSP 1 Score: 930 bits (2403), Expect = 0.0
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0
Query: 1 MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGP 60
MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGP
Sbjct: 1 MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFTASKGLKPPTVAGGWPLIGHLPLLAGP 60
Query: 61 ELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILG 120
ELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILG
Sbjct: 61 ELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILG 120
Query: 121 YNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDE 180
YNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDE
Sbjct: 121 YNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDE 180
Query: 181 QGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTL 240
QGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTL
Sbjct: 181 QGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTL 240
Query: 241 GDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVL 300
GDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVL
Sbjct: 241 GDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVL 300
Query: 301 EEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQE 360
EEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQE
Sbjct: 301 EEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQE 360
Query: 361 LDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEKGTWL 420
LDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEKGTWL
Sbjct: 361 LDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEKGTWL 420
Query: 421 FMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFG 465
FMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFG
Sbjct: 421 FMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFG 465
BLAST of MC01g_new0423 vs. ExPASy TrEMBL
Match:
A0A6J1BY79 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005812 PE=3 SV=1)
HSP 1 Score: 834 bits (2154), Expect = 2.29e-301
Identity = 420/500 (84.00%), Postives = 449/500 (89.80%), Query Frame = 0
Query: 1 MEPNAQFNWLSLITTLLAGVIAINFFRFLLKTFTA---SKGLKPPTVAGGWPLIGHLPLL 60
MEP+ QF+WL+LI TLL GVIAINF RFL KTFTA SKGLKPP+V GGWPLIGHLPLL
Sbjct: 1 MEPSPQFHWLNLIATLLGGVIAINFLRFLHKTFTAAAASKGLKPPSVPGGWPLIGHLPLL 60
Query: 61 AGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASK 120
GP+LPH+KL ALA+EYGPIFSIQLGI +AVVINSWEAAKQCFTTHDVA+S RP ILA++
Sbjct: 61 GGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFRPKILAAQ 120
Query: 121 ILGYNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKER 180
ILGYNYA+FAFGPYDSYWRKMRKTT LHLLS+RRLELLR+VRESEVNQAIKELY WKER
Sbjct: 121 ILGYNYAVFAFGPYDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKELYQIWKER 180
Query: 181 RDEQGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGL 240
RD+QGQ LVEMKQWVGDLTMNLILGVVAGKRYFG AMV EAEARAC +A KEFLRLMGL
Sbjct: 181 RDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMKEFLRLMGL 240
Query: 241 FTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVML 300
F LGD VPYLGWLDVGGHVKAMKRTSKELDH+LT+WLEEH+K RASG+SKEDKDFMDVML
Sbjct: 241 FILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKEDKDFMDVML 300
Query: 301 SVLEEETANVV-SDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRK 360
S LEE NVV SDS HSFD DTIIKATCLTMVLGGSDTT VVVTWALSLLLNN PAL K
Sbjct: 301 STLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLLNNHPALIK 360
Query: 361 VQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEK 420
VQ+E+DA IGRDREL ESD+ +LVY QAVVKETMRLYG GL +RETSEDFAIDGFHIEK
Sbjct: 361 VQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFAIDGFHIEK 420
Query: 421 GTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSL 480
GT LF+N WKIHRD RVWGEPLEFKPERFLTTH+DFDV GQQFELIPFGGGRRAC G+S
Sbjct: 421 GTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDVNGQQFELIPFGGGRRACPGLSF 480
Query: 481 GLQMVQLILGGLLQAFDIST 496
GLQMVQLILG LL AFDIS+
Sbjct: 481 GLQMVQLILGALLHAFDISS 500
BLAST of MC01g_new0423 vs. ExPASy TrEMBL
Match:
A0A6J1BV48 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005814 PE=3 SV=1)
HSP 1 Score: 698 bits (1801), Expect = 1.10e-249
Identity = 351/408 (86.03%), Postives = 373/408 (91.42%), Query Frame = 0
Query: 125 MFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQV 184
MF FGPY+SYWRKMRKTTN+HLLSNRRLELLRNVRESEV+QAIKELY WK+RRD QG+
Sbjct: 1 MFGFGPYNSYWRKMRKTTNVHLLSNRRLELLRNVRESEVSQAIKELYQIWKKRRDGQGRG 60
Query: 185 LVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTLGDVV 244
LVEMKQW+G+LTMNLILGVVA KRYFG AAMV E EARAC +ATKEFLRLMGLFTLGD
Sbjct: 61 LVEMKQWIGELTMNLILGVVASKRYFGPAAMVEEDEARACQKATKEFLRLMGLFTLGDAA 120
Query: 245 PYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVLEEET 304
P+LGWLDVGGHVKAMKRTSKELDH+LT+WLEEH+KDRASG SKEDKDFMDVMLS LEE
Sbjct: 121 PHLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKDRASGGSKEDKDFMDVMLSTLEETP 180
Query: 305 ANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDAR 364
VVSDS HSFD DTIIKATCLTMVLGG+DTTVVVVTW LSLLLNNRPALRKVQ+ELDA
Sbjct: 181 DAVVSDSIHSFDVDTIIKATCLTMVLGGNDTTVVVVTWTLSLLLNNRPALRKVQEELDAH 240
Query: 365 IGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAIDGFHIEKGTWLFMNL 424
IGR+REL ESD+ +LVY QAVVKETMRLYG VGL +RETS DFAIDGF IEKGTW+F+NL
Sbjct: 241 IGRERELKESDMSELVYLQAVVKETMRLYGAVGLILRETSVDFAIDGFQIEKGTWIFINL 300
Query: 425 WKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQLI 484
WKIHRDERVWGEPLEFKPERFLTTH DFDVKGQQFELIPFGGGRRACSG+S GLQMVQLI
Sbjct: 301 WKIHRDERVWGEPLEFKPERFLTTHIDFDVKGQQFELIPFGGGRRACSGISFGLQMVQLI 360
Query: 485 LGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALYD 532
LG LL AFDISTVEDAAVDMTGSIGLSNMKA+PLEVFIKPRL ALYD
Sbjct: 361 LGTLLHAFDISTVEDAAVDMTGSIGLSNMKATPLEVFIKPRLPFALYD 408
BLAST of MC01g_new0423 vs. ExPASy TrEMBL
Match:
A0A6P5SXI3 (cytochrome P450 CYP82D47-like OS=Prunus avium OX=42229 GN=LOC110762679 PE=3 SV=1)
HSP 1 Score: 608 bits (1569), Expect = 1.26e-212
Identity = 301/529 (56.90%), Postives = 388/529 (73.35%), Query Frame = 0
Query: 9 WLSLITTLLAGVIAINFFRFLL----KTFTASKGLKPPTVAGGWPLIGHLPLLAGPELPH 68
+L + T +AG++AI F + + + T +KG K P VAGGWPL+GHL L G +LPH
Sbjct: 4 YLPCLNTAIAGILAILLFSYFIIKRSSSATKAKGPKLPKVAGGWPLLGHLGLFRGSQLPH 63
Query: 69 IKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYA 128
I L++L ++YGP F+I +GI A+VI++WE AK CFTT+D+ +SSRP L +K LGYN+A
Sbjct: 64 IALASLVDKYGPAFTINIGIHSALVISTWEMAKDCFTTNDIVVSSRPATLGAKHLGYNFA 123
Query: 129 MFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQV 188
MF F PY YWR++RK T+L LLSNRRLELL+ VR SEV ++KELYT W +R++ G++
Sbjct: 124 MFGFTPYGPYWREIRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWTKRKESSGEL 183
Query: 189 LVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTLGDVV 248
LVEMKQW GDLT+N+I +VAGKRYF ++ E EAR C +A +EF L+GLF LGD V
Sbjct: 184 LVEMKQWFGDLTLNVIFRMVAGKRYFMNGSLNEEKEARRCQKAMREFFLLVGLFVLGDAV 243
Query: 249 PYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVLEEET 308
P+L WLD+GG KAMK+T+KELD ++T WLEEH++ R G+ D+DFMDVMLS ++
Sbjct: 244 PWLSWLDLGGQQKAMKKTAKELDSIVTEWLEEHKQKRTKGK---DQDFMDVMLSAID--- 303
Query: 309 ANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDAR 368
FD DT+IKATCL+++ GGSDTT+V +TWALSLLLNNR L+KV +ELD +
Sbjct: 304 ----GADVAGFDADTVIKATCLSLIAGGSDTTMVTLTWALSLLLNNRQVLKKVYEELDQQ 363
Query: 369 IGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLF-VRETSEDFAIDGFHIEKGTWLFMN 428
+G+ R L+ESDI LVY QA VKE MRL L RE +ED + G+H+ KGTWL +N
Sbjct: 364 VGKGRLLDESDINNLVYLQATVKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVN 423
Query: 429 LWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQL 488
LWKI D RVW +P+EF P+RFLTTHKD DV+GQQFEL+PFG GRRAC G+S GLQM L
Sbjct: 424 LWKIQTDPRVWADPMEFIPKRFLTTHKDVDVRGQQFELMPFGSGRRACPGISFGLQMTLL 483
Query: 489 ILGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALYD 532
L L +FD++T E+ VDMTGSIGL+NMK +PL+V +KPRLS LYD
Sbjct: 484 TLASFLHSFDVTTQENTPVDMTGSIGLTNMKLTPLDVLVKPRLSPNLYD 522
BLAST of MC01g_new0423 vs. ExPASy TrEMBL
Match:
M5XN66 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G387500 PE=3 SV=1)
HSP 1 Score: 600 bits (1548), Expect = 1.94e-209
Identity = 301/529 (56.90%), Postives = 386/529 (72.97%), Query Frame = 0
Query: 9 WLSLITTLLAGVIAINFFR-FLLKTFTAS---KGLKPPTVAGGWPLIGHLPLLAGPELPH 68
+L + T +AG++AI F F++K +++ KG K P VAGGWPL+GH L G +LPH
Sbjct: 4 YLPCLNTAIAGILAILLFSYFIIKRSSSAAKAKGPKLPKVAGGWPLLGHFGLFRGSQLPH 63
Query: 69 IKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYA 128
I L++L ++YGP F+I +GI A+VI++WEAAK CFTT+D+ +SSRP L +K LGYN+A
Sbjct: 64 IALASLVDKYGPAFTINIGIHSALVISTWEAAKDCFTTNDIVVSSRPATLGAKHLGYNFA 123
Query: 129 MFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQV 188
MF FGPY YWR+MRK T+L LLSNRRLELL+ VR SEV ++KELYT W +R++ G+
Sbjct: 124 MFGFGPYGPYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGER 183
Query: 189 LVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTLGDVV 248
LVEMKQW GDLT+N+I +VAGKR F + E EAR +A +EF L+GLF LGD V
Sbjct: 184 LVEMKQWFGDLTLNVIFRMVAGKRCFMNGNLSEEKEARRWQKAMREFFHLVGLFVLGDAV 243
Query: 249 PYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVLEEET 308
P+L WLD+GG KAMK+T+KELD ++ WLEEH++ R G+ D+DFMDVMLS ++
Sbjct: 244 PWLSWLDLGGQQKAMKKTAKELDSIVAEWLEEHKQKRTKGK---DQDFMDVMLSAID--- 303
Query: 309 ANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDAR 368
FD DT+IKATCL+++ GGSDTT+V +TW LSLLLNNR L+KV +ELD
Sbjct: 304 ----GRDVAGFDADTVIKATCLSLIAGGSDTTMVTLTWTLSLLLNNRQVLKKVYEELDQY 363
Query: 369 IGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLF-VRETSEDFAIDGFHIEKGTWLFMN 428
+G+ R L+ESDI LVY QA VKE MRL L RE +ED + G+H+ KGTWL +N
Sbjct: 364 VGKGRLLDESDINNLVYLQATVKEVMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVN 423
Query: 429 LWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQL 488
LWKI D RVW +P+EFKP+RFLTTHKD DV+GQQFEL+PFG GRRAC G+S GLQM L
Sbjct: 424 LWKIQTDPRVWADPMEFKPDRFLTTHKDVDVRGQQFELMPFGSGRRACPGISFGLQMTLL 483
Query: 489 ILGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALYD 532
L L +FD++T E+A VDMTGSIGL+N+K +PL+V +KPRLS LYD
Sbjct: 484 TLASFLHSFDVTTQENAPVDMTGSIGLTNIKLTPLDVLVKPRLSPNLYD 522
BLAST of MC01g_new0423 vs. TAIR 10
Match:
AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )
HSP 1 Score: 476.1 bits (1224), Expect = 3.7e-134
Identity = 248/526 (47.15%), Postives = 352/526 (66.92%), Query Frame = 0
Query: 14 TTLLAGVIAINFFRF--LLKTFTASKGLKPPTVAGGWPLIGHLPLLAGPE-LPHIKLSAL 73
T+L + + I F F L K K +K P +G WP+IGHL LL+G E L + L +
Sbjct: 3 TSLFSLFVPILVFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKM 62
Query: 74 ANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYAMFAFGP 133
A++YGP S++LG + V++S+E AK CFT +D A++SRP A+K +GY+ A+F F P
Sbjct: 63 ADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAP 122
Query: 134 YDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQVLVEMKQ 193
Y ++WR+MRK L LLSNRRL++L++VR SE++ +++LY+ W ++ + V+V++K
Sbjct: 123 YSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSE-PVMVDLKS 182
Query: 194 WVGDLTMNLILGVVAGKRYFGAAAMVAE--AEARACHRATKEFLRLMGLFTLGDVVPYLG 253
W+ D+++N+++ +VAGKRYFG ++ E EAR C + F L+G+FT+ D P LG
Sbjct: 183 WLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLG 242
Query: 254 WLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDR-ASGRSKEDKDFMDVMLSVLEEETANV 313
W D GH K MK+T +ELD +L RW+E HR+ R SG D DF+DVMLS+ E+
Sbjct: 243 WFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQ---GK 302
Query: 314 VSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDARIGR 373
S H D T IK+TCL ++LGGS+T+ +TWA+SLLLNN+ L+K Q E+D +GR
Sbjct: 303 FSHLQH--DAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGR 362
Query: 374 DRELNESDIGKLVYFQAVVKETMRLYGVVGLF-VRETSEDFAIDGFHIEKGTWLFMNLWK 433
DR + +SDI LVY QA++KET+RLY L RE ED + G+++ +GT + +N+WK
Sbjct: 363 DRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWK 422
Query: 434 IHRDERVWGEPLEFKPERFLTTH-KDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQLIL 493
I RD RV+ EP EF+PERF+T K+FDV+GQ FEL+PFG GRR+C G SL +Q++ L L
Sbjct: 423 IQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGL 482
Query: 494 GGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALY 532
LQ+FD+ TV D VDMT S GL+ KA+PLE+ I PRL LY
Sbjct: 483 ARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522
BLAST of MC01g_new0423 vs. TAIR 10
Match:
AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )
HSP 1 Score: 472.6 bits (1215), Expect = 4.1e-133
Identity = 246/527 (46.68%), Postives = 349/527 (66.22%), Query Frame = 0
Query: 14 TTLLAGVIAINFFRF--LLKTFTASKGLKPPTVAGGWPLIGHLPLLAGPE-LPHIKLSAL 73
T+L + + I F F L K K +K P +G WP+IGHL LL G E L + L +
Sbjct: 3 TSLFSLFVPILVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 62
Query: 74 ANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYAMFAFGP 133
A+ YGP S+QLG +A V++S+E AK CFT +D A++SRP A+K +GYN+A+F F P
Sbjct: 63 ADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAP 122
Query: 134 YDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQVLVEMKQ 193
Y ++WR+MRK + LLSNRRL++L++VR SE+ +K+LY+ W + + V+V++K
Sbjct: 123 YSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK-PVMVDLKS 182
Query: 194 WVGDLTMNLILGVVAGKRYFGAAAMVAE---AEARACHRATKEFLRLMGLFTLGDVVPYL 253
W+ D+T+N+I+ +VAGKRYFG V+ EA C +A +F L+G+FT+ D P L
Sbjct: 183 WLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTL 242
Query: 254 GWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRA-SGRSKEDKDFMDVMLSVLEEETAN 313
+ D+ GH K MK+T ELD +L RW+E HR+ R SG + D DF+DVM+S+ E+
Sbjct: 243 SFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKL- 302
Query: 314 VVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDARIG 373
S +D +T IK+TCL ++LGGSDT+ +TWA+SLLLNN+ L+K Q E+D +G
Sbjct: 303 ----SHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVG 362
Query: 374 RDRELNESDIGKLVYFQAVVKETMRLYGVVGLF-VRETSEDFAIDGFHIEKGTWLFMNLW 433
RDR + +SDI LVY QA++KET+RLY L RE ED + G+++ GT L +N+W
Sbjct: 363 RDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVW 422
Query: 434 KIHRDERVWGEPLEFKPERFLTTH-KDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQLI 493
KI RD +V+ EP EF+PERF+T K+FDV+GQ FEL+PFG GRR+C G SL +Q++ L
Sbjct: 423 KIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLG 482
Query: 494 LGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALY 532
L L +FD+ TV D VDM+ + GL+ KA+PLEV I PR+ L+
Sbjct: 483 LARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523
BLAST of MC01g_new0423 vs. TAIR 10
Match:
AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )
HSP 1 Score: 434.9 bits (1117), Expect = 9.5e-122
Identity = 241/527 (45.73%), Postives = 336/527 (63.76%), Query Frame = 0
Query: 14 TTLLAGVIAINFFRF--LLKTFTASKGLKPPTVAGGWPLIGHLPLLAGPE-LPHIKLSAL 73
T+L + ++I F F L K K +K P +G WP+IGHL LL G E L + L +
Sbjct: 3 TSLFSLFVSILVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 62
Query: 74 ANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYAMFAFGP 133
A+ YGP S++LG + V +S+E AK CFT +D A++S A+K +GY
Sbjct: 63 ADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASLMT-AAAKHMGY--------- 122
Query: 134 YDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQVLVEMKQ 193
+W +MRK + LLSNRRL++L NVR SE++ +K+LY+ W ++ + V+V++K
Sbjct: 123 --VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSE-PVMVDLKS 182
Query: 194 WVGDLTMNLILGVVAGKRYF---GAAAMVAEAEARACHRATKEFLRLMGLFTLGDVVPYL 253
W+ D+ N+I+ +VAGKRYF GA + EAR + +F L+G+FT+ D P L
Sbjct: 183 WLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKL 242
Query: 254 GWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDR-ASGRSKEDKDFMDVMLSVLEEETAN 313
GWLD+ GH K MK+T +ELD +L RW+E HR+ R SG D DF+DVMLS+ E+
Sbjct: 243 GWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKL- 302
Query: 314 VVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDARIG 373
S +D +T IK TCL ++LGGS+T+ +TWA+SLLLNN+ L+KVQ E+D +G
Sbjct: 303 ----SHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVG 362
Query: 374 RDRELNESDIGKLVYFQAVVKETMRLYGVVGLF-VRETSEDFAIDGFHIEKGTWLFMNLW 433
RDR + +SDI LVY QA++KET+RLY L RE ED + G+++ GT L +N+W
Sbjct: 363 RDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVW 422
Query: 434 KIHRDERVWGEPLEFKPERFLTTH-KDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQLI 493
KI RD +V+ EP EF+PERF+T KDFDV+GQ FEL+PFG GRR+C G SL +QM+ L
Sbjct: 423 KIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLG 482
Query: 494 LGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALY 532
L L +F++ TV D VDM+ S GL+ KA+PLEV I PRL L+
Sbjct: 483 LARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511
BLAST of MC01g_new0423 vs. TAIR 10
Match:
AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )
HSP 1 Score: 401.0 bits (1029), Expect = 1.5e-111
Identity = 228/528 (43.18%), Postives = 315/528 (59.66%), Query Frame = 0
Query: 10 LSLITTLLAGVIAINFFRFLLKTFTASKGLK--PPTVAGGWPLIGHLPLLAGPELPHIKL 69
+ LI L + I L+K+ K K P V G WPL+GHL L H+
Sbjct: 1 MDLIMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTF 60
Query: 70 SALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSRPNILASKILGYNYAMFA 129
A+A+ YGP+F +LG K ++INS E AK+ +T HD + RP + ASK+LGYN +
Sbjct: 61 GAMADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHD-KLLERPELTASKLLGYNDSFLT 120
Query: 130 FGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKELYTAWKERRDEQGQVLVE 189
F PY YWR++RK L S +++ R E + A + LY W++R + VLV+
Sbjct: 121 FSPYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVD 180
Query: 190 MKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATKEFLRLMGLFTLGDVVPYL 249
MKQ DLT N+ L +V+GKRYFG EAR C + +EFL LF L DV P L
Sbjct: 181 MKQEFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVL 240
Query: 250 GWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKEDKDFMDVMLSVLEEETANV 309
G+LD + MKRT+K LD + W+EEH K++ S + + D++D+++ +L ++
Sbjct: 241 GFLD-WKTKRGMKRTAKGLDKVAEGWIEEH-KNKRSDHGRSENDYLDILIKILGQDKIPG 300
Query: 310 VSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLNNRPALRKVQQELDARIGR 369
+SD+ T IKA CL +VL GS+T +VV+ WA+SLLLNN LRK Q+ELD++IG+
Sbjct: 301 LSDT------HTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGK 360
Query: 370 DRELNESDIGKLVYFQAVVKETMRLYGVVGLFV-RETSEDF--AIDGFHIEKGTWLFMNL 429
+R + E DI LVY QA+VKET RLY V L R EDF A H+ GT L ++
Sbjct: 361 ERVVEELDIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSA 420
Query: 430 WKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQFELIPFGGGRRACSGMSLGLQMVQLI 489
WKIHRD VW P +F+PERFLT++++ DV GQ ++ PFG GRR+C + LG++MV +
Sbjct: 421 WKIHRDPNVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYL 480
Query: 490 LGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLEVFIKPRLSHALYD 533
L L +FD++ VDMT S GL N KA+PLEV I PRL +LY+
Sbjct: 481 LVRFLHSFDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYE 519
BLAST of MC01g_new0423 vs. TAIR 10
Match:
AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )
HSP 1 Score: 372.9 bits (956), Expect = 4.4e-103
Identity = 207/492 (42.07%), Postives = 292/492 (59.35%), Query Frame = 0
Query: 42 PTVAGGWPLIGHLPLLAGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFT 101
P G PL GHL LL G +L KL+A++ ++GPIFS++LG + VV + + K CFT
Sbjct: 40 PEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFT 99
Query: 102 THDVAISSRPNILASKILGYNYAMFAFGPYDSYWRKMRKTTNLHLLSNRRLELLRNVRES 161
T+D+A ++RPNI + +GYN A PY YWR++RK +HL SN +E+L ++R S
Sbjct: 100 TNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSS 159
Query: 162 EVNQAIKELYTAWKERRDEQGQVLVEMKQWVGDLTMNLILGVVAGKRY-FGAAAMVAEAE 221
EVN IK LY + G +V++ LT N+IL + GKR FG ++
Sbjct: 160 EVNTLIKHLY------KGNGGTSIVKIDMLFEFLTFNIILRKMVGKRIGFGE----VNSD 219
Query: 222 ARACHRATKEFLRLMGLFTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKD 281
A K L + +GDV+P+LGWLD + MKR KELD + T+WL EH K
Sbjct: 220 EWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAKN-SQMKRLFKELDSVNTKWLHEHLKK 279
Query: 282 RASGRSKEDKDFMDVMLSVLEEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVV 341
R+ +++ MD++L +L E+ V+S D I+KAT L + L GSD+T + +
Sbjct: 280 RSRNEKDQERTIMDLLLDILPEDI--VISGHVR----DVIVKATILALTLTGSDSTSITL 339
Query: 342 TWALSLLLNNRPALRKVQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLF- 401
TWA+SLLLNN AL Q+E+D +G+ R + ESDI L Y QA+VKET RLY L
Sbjct: 340 TWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTG 399
Query: 402 VRETSEDFAIDGFHIEKGTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVKGQQF 461
+RE ED + G+ +EKGT L +N+WK+HRD ++W +P FKPERF+ + F
Sbjct: 400 IREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFMEDKS--QCEKSNF 459
Query: 462 ELIPFGGGRRACSGMSLGLQMVQLILGGLLQAFDISTVEDAAVDMTGSIGLSNMKASPLE 521
E IPFG GRR+C G++LGL++V +L LLQ F++ V D +DM GL+ K +P+E
Sbjct: 460 EYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEGPGLALPKINPVE 512
Query: 522 VFIKPRLSHALY 532
V + PRL LY
Sbjct: 520 VVVMPRLDPKLY 512
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| O49858 | 3.3e-143 | 48.86 | Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1 | [more] |
| Q43068 | 6.4e-139 | 46.35 | Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2 | [more] |
| O49859 | 4.6e-137 | 46.98 | Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1 | [more] |
| A0A0N7F297 | 9.5e-135 | 49.62 | Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP... | [more] |
| O49394 | 5.2e-133 | 47.15 | Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022132489.1 | 0.0 | 100.00 | cytochrome P450 CYP82D47-like [Momordica charantia] >XP_022132490.1 cytochrome P... | [more] |
| XP_022133138.1 | 4.74e-301 | 84.00 | cytochrome P450 CYP82D47-like [Momordica charantia] | [more] |
| XP_022133139.1 | 2.27e-249 | 86.03 | cytochrome P450 CYP82D47-like [Momordica charantia] | [more] |
| XP_038883369.1 | 8.01e-246 | 66.54 | cytochrome P450 CYP82D47-like [Benincasa hispida] | [more] |
| KAG2663852.1 | 3.68e-213 | 58.72 | hypothetical protein I3760_16G051800 [Carya illinoinensis] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1BSL4 | 0.0 | 100.00 | cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005334 PE=... | [more] |
| A0A6J1BY79 | 2.29e-301 | 84.00 | cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005812 PE=... | [more] |
| A0A6J1BV48 | 1.10e-249 | 86.03 | cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005814 PE=... | [more] |
| A0A6P5SXI3 | 1.26e-212 | 56.90 | cytochrome P450 CYP82D47-like OS=Prunus avium OX=42229 GN=LOC110762679 PE=3 SV=1 | [more] |
| M5XN66 | 1.94e-209 | 56.90 | Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G387500 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G31970.1 | 3.7e-134 | 47.15 | cytochrome P450, family 82, subfamily C, polypeptide 2 | [more] |
| AT4G31940.1 | 4.1e-133 | 46.68 | cytochrome P450, family 82, subfamily C, polypeptide 4 | [more] |
| AT4G31950.1 | 9.5e-122 | 45.73 | cytochrome P450, family 82, subfamily C, polypeptide 3 | [more] |
| AT2G25160.1 | 1.5e-111 | 43.18 | cytochrome P450, family 82, subfamily F, polypeptide 1 | [more] |
| AT3G25180.1 | 4.4e-103 | 42.07 | cytochrome P450, family 82, subfamily G, polypeptide 1 | [more] |
