MC01g_new0404 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g_new0404
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPurple acid phosphatase
LocationMC01: 16814463 .. 16817481 (+)
RNA-Seq ExpressionMC01g_new0404
SyntenyMC01g_new0404
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAGAAATACCAATTGGATTTCTCTCTTTGAATTTTGACGAATTAGTAATTAGTATCCAAACAGAAAACAAATATTCCTAATTTCACGTATATAAAGCCACCCGTCCATCGGCATTTACGGAGTATGGATTAAATTACATCTCTCTACCCTATCTATAAAATTAATATAAAATTCTCATCTCTCCCTCTCAAAAACCCTCCAATTCTTCCCTCTTCGAATTCACCGCCATGGCTGCAATTTCGCAGTTCATAGCTCTGCTTCTATTAGCTCTATTGCTCTCCGTCGCCGACGTCTGCAATGGAGGTATTACCAGTAAATACACCAGGAAACCTGAAGCTTCCGTCGACATGCCCGTCGAAGCGTTCCCCCCGCCCCCTGGTTTCAACGCACCGGAGCAGGTCTCCGATATGGAAGATTTACAGATAGAATAGTTTCAATTTTTTTTTTCTTTTAATCTTTTCATTTTATGGAAGGTTTTGTTTGTTTTTGGTGAATGTGCAGGTTCACATAACCCAGGGAGATCGCCATGGCAGAGGCATTATAATCTCATGGGTAACGCCACGGTCGCCCCACCCGAATGTTGTCAGATATTGGGCTGAAGATAGCGACGAAAATCAGAAGCAAAAAGCACATTCCAGAATCACTACTTACAAATATTACAATTACACTTCTGGATTCATACACCACGCCACCATTGACGAACTTGAGGTTCGCTCTGTTAAAACCCTCTGCCTTTTTATTTCCAGTCTCGAAAACTAAGGTTCCAAAATGTTGAATTTCAGTACGATACTAAATACTTCTATGAGCTCGGGAGCGGCAACACGAAGCGCGGATTTTCGTTCAGAACGCCTCCCAAGCCCGGGCCTGATGTTCCGTACACATTCGGCATTATTGGTTTGTCTTCTTCCTCGCTTGTGATTCGATTGCATTCTCTGTTTTATAAAGATAAATTAAAGAAGCAGTTGCAATATCTCTGCAGGCGATCTTGGACAGACTTATGATTCTAATAAAACTTTTGAGCATTATTTTACAAACCCCAAGGGACAAGCCGTGCTGTTCGTCGGAGATCTTTCTTACGCCGACAACCATCCGTACCACGACAACAGGAAGTGGGATACATGGGCTCGATTTGTCGAGAAATCAACTGCCTATCAGCCGTGGATTTGGACCGCCGGCAATCACGAAATGGATTTCGCTCCAGAAATTGTAAGAACAACTGCACAAGATTACCATCTTTCTACTTCTTCAGTTTACAATTTCCTCCTACTTTGCCCCCTAGATCTTTAGAATCGAAAGATTATATATAGAATGATAGGAAATTTTGAAGCTAACCCAATTGTGTTGTTTCAGGGAGAAAACACCCCTTTCAAGCCTTATACGCATCGGTATCACGTTCCTTACAAAACATCACAAAGCTCGTCACCACTGTGGTATTCGATCAAGCGTGCATCTGCCTACATTATAGTCCTTTCGTCCTACTCTGCTTATGGTACGTGTTGATTTGGTGATGAGGGTATTGAGTTACATTCGAAAACAGAGCTAAAAGTGGAGTTTTATTGATAAATCAGGTACATATACTCCGCAATACCAGTGGCTGGAGAATGAGTTTTTGAAGGTGAAGAGAGATGAGACTCCATGGTTGATTGTTATGCTTCATTCTCCCTGGTATAATAGTTACGACTACCATTATATGGAAGGAGAGAGCATGCGGGTTATGTTCGAGTCCTGGTTTGTTCAGAACAAAGTTGATCTTGTTCTTTCTGGCCACGTCCATGCTTATGAGCGTTCGGTATGTGAACTTATTTTCTTTGAGAAAATTGTGAGATGGGGATTTGAAGAAATATGAAGCGAAGTGATTGTTTATTTGTGGCAGGAGCGAGTGTCCAATGTGAGATACAACATCACAAATGGATTGAGCACTCCAATTAGAGACGTTAATGCACCAATTTACATAACAGTCGGTGATGGTGGAAATATTGAAGGTCTTGCTAACCGGTGAGTCTTTTTCACATCTTTGCTTTTTCCTTCGAAGACGAAAAGAACTTATTTTTTAATCTCATATGTTGATGAAGTGTGAAATTCAATCAAATTGTTGATTTCATTAAATTAAACTTTAAATTGGATTAAGTGACTATTTACAGTTCAACAGCACTTTTGAACTTCTAGAATATATGCGGTTGACTAATAATTAACCATGGTTACTTTAATAGTTTTTTCCCTTTTGGAAATTATGTTACTTCTATGGTTGTAGAGTTGACTTGCATTCTTAGGTTAGAATACCATTATTTTTTAGTTAGCACCTATCTTAGTGCCGACTTAGTTGAAAATGTTGACTTAATGGTGGTAAGCACGAAATTGAGTCGGAGCACTCCTAAACGTTACACTACTTTTTAATAAATGTGTGGTTTGGAATAACCCAAAAGATGAAGAGTTACTCTTTGTCTAAATTCTCAACCTTTTATTATTTGATCTTAGATAAAATTTCTAACTTTGGTTAGTTAATACACACCCAAGTAAATTGCTAAAAGCCAATCAAAATGAGCTTTTTAGTTTCCTTAGAAAAAGAAAACTACATAGTACATATATAGTTTGATCATATATATATATATATATATATATAACATTAGAGTTGGTTGGTTTATGCAGTTTCACAGAGCCACAACCAAGTTACTCAGCGTATCGGGAGGCAAGCTTCGGGCACGCAATGCTTGAAATCAAGAATCGAACTCATGCATACTATACATGGCACCGCAACGAAGACCATGAGCCTGTTGCTGCTGATTCACTTTGGATATATAACAGATACTGGTTCCCACAAGACGAGGCTTCTTCTCAATAGACTTTCCAAAAAGAAAAAGATAACTTTTGCGGTTTTTAGGTTGTAGTGCAGTGTTTTGTGGTGGATTTTAGCATCTTCATTCCCTATTGCACACACAGTGAATAAAATAACCATTTGTTCGTGCAAAACGAAGGGCACCCATTCTCTTTCTTTTATTTTAATATCGTTTTCAAAGGGG

mRNA sequence

ATGGCTGCAATTTCGCAGTTCATAGCTCTGCTTCTATTAGCTCTATTGCTCTCCGTCGCCGACGTCTGCAATGGAGGTATTACCAGTAAATACACCAGGAAACCTGAAGCTTCCGTCGACATGCCCGTCGAAGCGTTCCCCCCGCCCCCTGGTTTCAACGCACCGGAGCAGGTTCACATAACCCAGGGAGATCGCCATGGCAGAGGCATTATAATCTCATGGGTAACGCCACGGTCGCCCCACCCGAATGTTGTCAGATATTGGGCTGAAGATAGCGACGAAAATCAGAAGCAAAAAGCACATTCCAGAATCACTACTTACAAATATTACAATTACACTTCTGGATTCATACACCACGCCACCATTGACGAACTTGAGTACGATACTAAATACTTCTATGAGCTCGGGAGCGGCAACACGAAGCGCGGATTTTCGTTCAGAACGCCTCCCAAGCCCGGGCCTGATGTTCCGTACACATTCGGCATTATTGGCGATCTTGGACAGACTTATGATTCTAATAAAACTTTTGAGCATTATTTTACAAACCCCAAGGGACAAGCCGTGCTGTTCGTCGGAGATCTTTCTTACGCCGACAACCATCCGTACCACGACAACAGGAAGTGGGATACATGGGCTCGATTTGTCGAGAAATCAACTGCCTATCAGCCGTGGATTTGGACCGCCGGCAATCACGAAATGGATTTCGCTCCAGAAATTGGAGAAAACACCCCTTTCAAGCCTTATACGCATCGGTATCACGTTCCTTACAAAACATCACAAAGCTCGTCACCACTGTGGTATTCGATCAAGCGTGCATCTGCCTACATTATAGTCCTTTCGTCCTACTCTGCTTATGGTACATATACTCCGCAATACCAGTGGCTGGAGAATGAGTTTTTGAAGGTGAAGAGAGATGAGACTCCATGGTTGATTGTTATGCTTCATTCTCCCTGGTATAATAGTTACGACTACCATTATATGGAAGGAGAGAGCATGCGGGTTATGTTCGAGTCCTGGTTTGTTCAGAACAAAGTTGATCTTGTTCTTTCTGGCCACGTCCATGCTTATGAGCGTTCGGAGCGAGTGTCCAATGTGAGATACAACATCACAAATGGATTGAGCACTCCAATTAGAGACGTTAATGCACCAATTTACATAACAGTCGGTGATGGTGGAAATATTGAAGGTCTTGCTAACCGTTTCACAGAGCCACAACCAAGTTACTCAGCGTATCGGGAGGCAAGCTTCGGGCACGCAATGCTTGAAATCAAGAATCGAACTCATGCATACTATACATGGCACCGCAACGAAGACCATGAGCCTGTTGCTGCTGATTCACTTTGGATATATAACAGATACTGGTTCCCACAAGACGAGGCTTCTTCTCAATAG

Coding sequence (CDS)

ATGGCTGCAATTTCGCAGTTCATAGCTCTGCTTCTATTAGCTCTATTGCTCTCCGTCGCCGACGTCTGCAATGGAGGTATTACCAGTAAATACACCAGGAAACCTGAAGCTTCCGTCGACATGCCCGTCGAAGCGTTCCCCCCGCCCCCTGGTTTCAACGCACCGGAGCAGGTTCACATAACCCAGGGAGATCGCCATGGCAGAGGCATTATAATCTCATGGGTAACGCCACGGTCGCCCCACCCGAATGTTGTCAGATATTGGGCTGAAGATAGCGACGAAAATCAGAAGCAAAAAGCACATTCCAGAATCACTACTTACAAATATTACAATTACACTTCTGGATTCATACACCACGCCACCATTGACGAACTTGAGTACGATACTAAATACTTCTATGAGCTCGGGAGCGGCAACACGAAGCGCGGATTTTCGTTCAGAACGCCTCCCAAGCCCGGGCCTGATGTTCCGTACACATTCGGCATTATTGGCGATCTTGGACAGACTTATGATTCTAATAAAACTTTTGAGCATTATTTTACAAACCCCAAGGGACAAGCCGTGCTGTTCGTCGGAGATCTTTCTTACGCCGACAACCATCCGTACCACGACAACAGGAAGTGGGATACATGGGCTCGATTTGTCGAGAAATCAACTGCCTATCAGCCGTGGATTTGGACCGCCGGCAATCACGAAATGGATTTCGCTCCAGAAATTGGAGAAAACACCCCTTTCAAGCCTTATACGCATCGGTATCACGTTCCTTACAAAACATCACAAAGCTCGTCACCACTGTGGTATTCGATCAAGCGTGCATCTGCCTACATTATAGTCCTTTCGTCCTACTCTGCTTATGGTACATATACTCCGCAATACCAGTGGCTGGAGAATGAGTTTTTGAAGGTGAAGAGAGATGAGACTCCATGGTTGATTGTTATGCTTCATTCTCCCTGGTATAATAGTTACGACTACCATTATATGGAAGGAGAGAGCATGCGGGTTATGTTCGAGTCCTGGTTTGTTCAGAACAAAGTTGATCTTGTTCTTTCTGGCCACGTCCATGCTTATGAGCGTTCGGAGCGAGTGTCCAATGTGAGATACAACATCACAAATGGATTGAGCACTCCAATTAGAGACGTTAATGCACCAATTTACATAACAGTCGGTGATGGTGGAAATATTGAAGGTCTTGCTAACCGTTTCACAGAGCCACAACCAAGTTACTCAGCGTATCGGGAGGCAAGCTTCGGGCACGCAATGCTTGAAATCAAGAATCGAACTCATGCATACTATACATGGCACCGCAACGAAGACCATGAGCCTGTTGCTGCTGATTCACTTTGGATATATAACAGATACTGGTTCCCACAAGACGAGGCTTCTTCTCAATAG

Protein sequence

MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHITQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHATIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASSQ
Homology
BLAST of MC01g_new0404 vs. ExPASy Swiss-Prot
Match: Q09131 (Purple acid phosphatase OS=Glycine max OX=3847 GN=PAP PE=1 SV=2)

HSP 1 Score: 679.1 bits (1751), Expect = 3.5e-194
Identity = 310/456 (67.98%), Postives = 371/456 (81.36%), Query Frame = 0

Query: 10  LLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEA--FPPPPGFNAPEQVHITQGDRHG 69
           LL LAL+LS   +CNGG +S + RK E +VDMP+++  F  PPG+NAP+QVHITQGD  G
Sbjct: 7   LLALALVLSACVMCNGGSSSPFIRKVEKTVDMPLDSDVFAVPPGYNAPQQVHITQGDLVG 66

Query: 70  RGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHATIDELEY 129
           + +I+SWVT   P  + V YW+E+SD  +K+ A  ++ TY+++NY+SGFIHH TI  LEY
Sbjct: 67  KAVIVSWVTVDEPGSSEVHYWSENSD--KKKIAEGKLVTYRFFNYSSGFIHHTTIRNLEY 126

Query: 130 DTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNP-KGQ 189
            TKY+YE+G GNT R F F TPP+ GPDVPYTFG+IGDLGQ++DSNKT  HY  NP KGQ
Sbjct: 127 KTKYYYEVGLGNTTRQFWFVTPPEIGPDVPYTFGLIGDLGQSFDSNKTLSHYELNPRKGQ 186

Query: 190 AVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTPFK 249
            VLFVGDLSYADN+P HDN +WD+W RF E+S AYQPWIWTAGNHE  FAPEIGE  PFK
Sbjct: 187 TVLFVGDLSYADNYPNHDNIRWDSWGRFTERSVAYQPWIWTAGNHENHFAPEIGETVPFK 246

Query: 250 PYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKRDE 309
           PYTHRYHVPYK SQS+SP WYSIKRASA+IIVL+SYSAYG YTPQY+WLE E  KV R E
Sbjct: 247 PYTHRYHVPYKASQSTSPFWYSIKRASAHIIVLASYSAYGKYTPQYKWLEKELPKVNRTE 306

Query: 310 TPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSNVR 369
           TPWLIV++HSPWYNSY+YHYMEGE+MRVM+E WFVQ KVD+V +GHVHAYERSERVSNV 
Sbjct: 307 TPWLIVLMHSPWYNSYNYHYMEGETMRVMYEPWFVQYKVDVVFAGHVHAYERSERVSNVA 366

Query: 370 YNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIKNR 429
           YNI NGL  P+ D +AP+YIT+GDGG +EGLA   TEPQP YSA+REASFGHA+ +I NR
Sbjct: 367 YNIVNGLCAPVNDKSAPVYITIGDGGTLEGLATNMTEPQPKYSAFREASFGHAIFDITNR 426

Query: 430 THAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASS 463
           THA+Y+WHRN+D   V ADSLW +NRYW P D++++
Sbjct: 427 THAHYSWHRNQDGVAVEADSLWSFNRYWHPVDDSTA 460

BLAST of MC01g_new0404 vs. ExPASy Swiss-Prot
Match: Q9SIV9 (Purple acid phosphatase 10 OS=Arabidopsis thaliana OX=3702 GN=PAP10 PE=2 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 6.0e-194
Identity = 310/458 (67.69%), Postives = 369/458 (80.57%), Query Frame = 0

Query: 8   IALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEA--FPPPPGFNAPEQVHITQGDR 67
           I L+L  +L S+  +CNGGITS+Y RK EA+VDMP+++  F  P G+NAP+QVHITQGD 
Sbjct: 12  IVLVLCCVLNSL--LCNGGITSRYVRKLEATVDMPLDSDVFRVPCGYNAPQQVHITQGDV 71

Query: 68  HGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHATIDEL 127
            G+ +I+SWVT  +   N V YW E+S   +K KAH +  TYK+YNYTSGFIHH  I  L
Sbjct: 72  EGKAVIVSWVTQEAKGSNKVIYWKENS--TKKHKAHGKTNTYKFYNYTSGFIHHCPIRNL 131

Query: 128 EYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNP-K 187
           EYDTKY+Y LG G T+R F F TPP+ GPDVPYTFG+IGDLGQ+YDSN T  HY  NP K
Sbjct: 132 EYDTKYYYVLGVGQTERKFWFFTPPEIGPDVPYTFGLIGDLGQSYDSNITLTHYENNPTK 191

Query: 188 GQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTP 247
           GQAVLFVGD+SYAD +P HDNR+WD+W RF E+STAYQPWIWT GNHE+DFAPEIGEN P
Sbjct: 192 GQAVLFVGDISYADTYPDHDNRRWDSWGRFAERSTAYQPWIWTTGNHELDFAPEIGENRP 251

Query: 248 FKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKR 307
           FKP+THRY  PY++S S+ P WYSIKR  AYIIVL+SYSAYG YTPQYQWLE EF KV R
Sbjct: 252 FKPFTHRYRTPYRSSGSTEPFWYSIKRGPAYIIVLASYSAYGKYTPQYQWLEEEFPKVNR 311

Query: 308 DETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSN 367
            ETPWLIV++HSPWYNSYDYHYMEGE+MRVM+E+WFV+ KVD+V +GHVHAYERSERVSN
Sbjct: 312 TETPWLIVLMHSPWYNSYDYHYMEGETMRVMYEAWFVKYKVDVVFAGHVHAYERSERVSN 371

Query: 368 VRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIK 427
           + YN+ NG+ TP++D +AP+YIT+GDGGNIEGLA + TEPQP YSA+REASFGHA+  IK
Sbjct: 372 IAYNVVNGICTPVKDQSAPVYITIGDGGNIEGLATKMTEPQPKYSAFREASFGHAIFSIK 431

Query: 428 NRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASS 463
           NRTHA+Y WHRN D   V  D +W YNR+W P D++ S
Sbjct: 432 NRTHAHYGWHRNHDGYAVEGDRMWFYNRFWHPVDDSPS 465

BLAST of MC01g_new0404 vs. ExPASy Swiss-Prot
Match: O23244 (Purple acid phosphatase 25 OS=Arabidopsis thaliana OX=3702 GN=PAP25 PE=2 SV=2)

HSP 1 Score: 674.5 bits (1739), Expect = 8.7e-193
Identity = 317/456 (69.52%), Postives = 373/456 (81.80%), Query Frame = 0

Query: 10  LLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHITQGDRHGRG 69
           +LL+ + LS+A V N G TS + R  + S +M +E FP P G NAPEQVHI QGD +GRG
Sbjct: 6   ILLVFVFLSIATVINSGTTSNFVRTAQPSTEMSLETFPSPAGHNAPEQVHIVQGDYNGRG 65

Query: 70  IIISWVTPRS-PHPNVVRYW-AEDSD-ENQKQKAHSRITTYKYYNYTSGFIHHATIDELE 129
           IIISWVTP +    NVV YW A D D + +K++ H+  ++Y++Y+YTSGF+HHATI  LE
Sbjct: 66  IIISWVTPLNLAGSNVVTYWKAVDGDVKPKKKRGHASTSSYRFYDYTSGFLHHATIKGLE 125

Query: 130 YDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNPKGQ 189
           YDTKY YE+G+  + R FSF +PPK GPDVPYTFGIIGDLGQT  SN+T  HY +NPKGQ
Sbjct: 126 YDTKYIYEVGTDGSVRQFSFTSPPKVGPDVPYTFGIIGDLGQTLASNETLYHYMSNPKGQ 185

Query: 190 AVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTPFK 249
           AVLF GDLSYAD+HP HD RKWD+W RFVE   AYQ +I+ AGNHE+DF P IGE   FK
Sbjct: 186 AVLFPGDLSYADDHPNHDQRKWDSWGRFVEPCAAYQTFIYAAGNHEIDFVPNIGEPHAFK 245

Query: 250 PYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKRDE 309
           PY HRYH  YK S+S SPLWYSI+RASA+IIVLSSYSAYG YTPQY WLE E  KV R+E
Sbjct: 246 PYIHRYHNAYKASKSISPLWYSIRRASAHIIVLSSYSAYGKYTPQYVWLEQELKKVNREE 305

Query: 310 TPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSNVR 369
           TPWLIVM+HSPWYNS +YHYMEGESMR MFESWFV +KVDLVLSGHVH+YERSERVSN++
Sbjct: 306 TPWLIVMVHSPWYNSNNYHYMEGESMRAMFESWFVNSKVDLVLSGHVHSYERSERVSNIK 365

Query: 370 YNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIKNR 429
           YNITNGLS P++D +APIYIT+GDGGNIEG+AN FT+PQPSYSAYREASFGHA+LEI NR
Sbjct: 366 YNITNGLSYPVKDPSAPIYITIGDGGNIEGIANSFTDPQPSYSAYREASFGHAVLEIYNR 425

Query: 430 THAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASS 463
           THAYYTWHRN+D+EPVAADS+ ++NRY+FP +E  S
Sbjct: 426 THAYYTWHRNQDNEPVAADSIMLHNRYFFPVEELES 461

BLAST of MC01g_new0404 vs. ExPASy Swiss-Prot
Match: Q9C510 (Purple acid phosphatase 6 OS=Arabidopsis thaliana OX=3702 GN=PAP6 PE=2 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 1.9e-192
Identity = 310/452 (68.58%), Postives = 370/452 (81.86%), Query Frame = 0

Query: 15  LLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHITQGDRHGRGIIISW 74
           L LS+  V NGGITSK+ R+   S++M ++ FP P G+N PEQVH+TQGD  GRG+I+SW
Sbjct: 10  LFLSITTVINGGITSKFVRQALPSIEMSLDTFPSPGGYNTPEQVHLTQGDHDGRGMIVSW 69

Query: 75  VTPRS-PHPNVVRYWAEDSDEN---QKQKAHSRITTYKYYNYTSGFIHHATIDELEYDTK 134
           VTP +    NVV YW   +  +    K++AH+   +Y++Y+Y+SGF+HHATI  LEYDTK
Sbjct: 70  VTPLNLAGSNVVTYWIATNGSDVKPAKKRAHASTKSYRFYDYSSGFLHHATIKGLEYDTK 129

Query: 135 YFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNPKGQAVLF 194
           Y YE+G+  + R FSF TPPK GPDVPYTFGIIGDLGQTY SN+T  HY +NPKGQAVLF
Sbjct: 130 YIYEVGTDKSVRQFSFTTPPKIGPDVPYTFGIIGDLGQTYASNETLYHYMSNPKGQAVLF 189

Query: 195 VGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTPFKPYTH 254
            GDLSYAD+HP HD RKWDTW RF+E   AYQP+I+ AGNHE+DF P IGE   FKPYTH
Sbjct: 190 AGDLSYADDHPNHDQRKWDTWGRFMEPCAAYQPFIFAAGNHEIDFVPNIGEPHAFKPYTH 249

Query: 255 RYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKRDETPWL 314
           RY   YK SQS+SPLWYS++RASA+IIVLSSYSAYG YTPQY WLE E   V R+ETPWL
Sbjct: 250 RYPNAYKASQSTSPLWYSVRRASAHIIVLSSYSAYGKYTPQYIWLEQELKNVNREETPWL 309

Query: 315 IVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSNVRYNIT 374
           IV++HSPWYNS +YHYMEGESMRVMFESW V +KVDLVLSGHVHAYERSER+SN++YNIT
Sbjct: 310 IVIVHSPWYNSNNYHYMEGESMRVMFESWLVNSKVDLVLSGHVHAYERSERISNIKYNIT 369

Query: 375 NGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIKNRTHAY 434
           NGLS+P++D NAPIYIT+GDGGNIEG+AN F +PQPSYSAYREASFGHA+LEI NRTHA 
Sbjct: 370 NGLSSPVKDPNAPIYITIGDGGNIEGIANSFVDPQPSYSAYREASFGHAVLEIMNRTHAQ 429

Query: 435 YTWHRNEDHEPVAADSLWIYNRYWFPQDEASS 463
           YTWHRN+D+EPVAADS+ ++NR++FP +E  S
Sbjct: 430 YTWHRNQDNEPVAADSIMLHNRHFFPVEEIVS 461

BLAST of MC01g_new0404 vs. ExPASy Swiss-Prot
Match: Q9SDZ9 (Purple acid phosphatase 2 OS=Ipomoea batatas OX=4120 GN=PAP2 PE=1 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 2.5e-192
Identity = 309/455 (67.91%), Postives = 368/455 (80.88%), Query Frame = 0

Query: 11  LLLALLLSVADVCNGGITSKYTRKPEASVDMPVEA--FPPPPGFNAPEQVHITQGDRHGR 70
           LL  +L +V +    GITS + RK E +VDMP+++  F  PPG+NAP+QVHITQGD  G+
Sbjct: 10  LLAVVLAAVMNAAIAGITSSFIRKVEKTVDMPLDSDVFRVPPGYNAPQQVHITQGDHVGK 69

Query: 71  GIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHATIDELEYD 130
            +I+SWVT   P  + V YW+E+S    K+ A   I TY Y+NYTSG+IHH TI  LEY+
Sbjct: 70  AMIVSWVTVDEPGSSKVVYWSENS--QHKKVARGNIRTYTYFNYTSGYIHHCTIRNLEYN 129

Query: 131 TKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNP-KGQA 190
           TKY+YE+G GNT R F F TPP+ GPDVPYTFG+IGDLGQ++DSN+T  HY  NP KGQA
Sbjct: 130 TKYYYEVGIGNTTRSFWFTTPPEVGPDVPYTFGLIGDLGQSFDSNRTLTHYERNPIKGQA 189

Query: 191 VLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTPFKP 250
           VLFVGDLSYADN+P HDN +WDTW RFVE+STAYQPWIWTAGNHE+DFAPEIGE  PFKP
Sbjct: 190 VLFVGDLSYADNYPNHDNVRWDTWGRFVERSTAYQPWIWTAGNHEIDFAPEIGETKPFKP 249

Query: 251 YTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKRDET 310
           +T RYHVPYK S S+   WY IKRASAYIIVLSSYSAYG YTPQY+WLE E  KV R ET
Sbjct: 250 FTKRYHVPYKASGSTETFWYPIKRASAYIIVLSSYSAYGKYTPQYKWLEEELPKVNRTET 309

Query: 311 PWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSNVRY 370
           PWLIV++HSPWYNSY+YHYMEGE+MRVM+E WFVQ+KVDLV +GHVHAYERSERVSNV Y
Sbjct: 310 PWLIVLMHSPWYNSYNYHYMEGETMRVMYEPWFVQHKVDLVFAGHVHAYERSERVSNVAY 369

Query: 371 NITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIKNRT 430
           +I NG  TP+RD +AP+YIT+GDGGN+EGLA   T+PQP YSA+REASFGHA L+IKNRT
Sbjct: 370 DIVNGKCTPVRDQSAPVYITIGDGGNLEGLATNMTDPQPEYSAFREASFGHATLDIKNRT 429

Query: 431 HAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASS 463
           HAYY+WHRN+D   V ADS+W+ NR+W P D++++
Sbjct: 430 HAYYSWHRNQDGYAVEADSMWVSNRFWHPVDDSTT 462

BLAST of MC01g_new0404 vs. NCBI nr
Match: XP_022132405.1 (purple acid phosphatase 5 [Momordica charantia])

HSP 1 Score: 978 bits (2529), Expect = 0.0
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI
Sbjct: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA
Sbjct: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF
Sbjct: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG
Sbjct: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL
Sbjct: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE
Sbjct: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM
Sbjct: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASSQ 463
           LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASSQ
Sbjct: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASSQ 463

BLAST of MC01g_new0404 vs. NCBI nr
Match: KAG7026765.1 (Purple acid phosphatase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 851 bits (2199), Expect = 2.58e-310
Identity = 391/460 (85.00%), Postives = 428/460 (93.04%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAISQFI LLL+   +S+A++CNGGITSK+ RK EASVDMP EAFPPP G+NAPEQVHI
Sbjct: 1   MAAISQFIVLLLV---ISLANLCNGGITSKFMRKLEASVDMPAEAFPPPAGYNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDRHGRG+IISWVTP SP P VVRYWA D D N++ KAHSRITTYKYYNYTS FIHHA
Sbjct: 61  TQGDRHGRGVIISWVTPLSPKPTVVRYWAADGDGNRRNKAHSRITTYKYYNYTSAFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TI+ LEYDTKYFYELGSGN  R F F TPP+ GPDVPYTFGIIGDLGQTYDSN+TFEHY+
Sbjct: 121 TINNLEYDTKYFYELGSGNATRRFFFTTPPRVGPDVPYTFGIIGDLGQTYDSNQTFEHYY 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           +NPKGQAVLF GDLSYADNHP+HDNRKWDTW RFVEKSTAYQPWIWTAGNHE+DFAP+IG
Sbjct: 181 SNPKGQAVLFAGDLSYADNHPFHDNRKWDTWGRFVEKSTAYQPWIWTAGNHEIDFAPQIG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKPYTHRYHVPYKT+QS+SPLWYSIKRASAYIIVLSSYSAYG YTPQY+WL+ EF 
Sbjct: 241 ENTPFKPYTHRYHVPYKTAQSTSPLWYSIKRASAYIIVLSSYSAYGKYTPQYEWLQKEFK 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKRDETPWLIVMLH+PWYNSY+YHYMEGESMRVMFESWF+QNKVDLVLSGH+HAYERSE
Sbjct: 301 KVKRDETPWLIVMLHAPWYNSYNYHYMEGESMRVMFESWFIQNKVDLVLSGHIHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITN L +P+RD+NAPIYIT+GDGGNIEGLAN FTEPQPSYSA+REASFGHA+
Sbjct: 361 RVSNVRYNITNRLCSPVRDINAPIYITIGDGGNIEGLANEFTEPQPSYSAFREASFGHAI 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEA 460
           LEIKNRTHAYYTWHRN D+EPV+ADSLWIYNRYW+P+DE+
Sbjct: 421 LEIKNRTHAYYTWHRNHDNEPVSADSLWIYNRYWYPEDES 457

BLAST of MC01g_new0404 vs. NCBI nr
Match: XP_022963133.1 (purple acid phosphatase 5-like [Cucurbita moschata])

HSP 1 Score: 847 bits (2189), Expect = 8.61e-309
Identity = 390/460 (84.78%), Postives = 426/460 (92.61%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAISQFI LLL    +S+A++CNGGITSK+ RK EASVDMP EAFPPP G+NAPEQVHI
Sbjct: 1   MAAISQFIVLLLS---ISLANLCNGGITSKFMRKLEASVDMPAEAFPPPAGYNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDRHGRG+IISWVTP SP P VVRYWA D D N++ KAHSRITTYKYYNYTS FIHHA
Sbjct: 61  TQGDRHGRGVIISWVTPLSPKPTVVRYWAADGDGNRRNKAHSRITTYKYYNYTSAFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TI+ LEYDTKYFYELGSGN  R F F TPP+  PDVPYTFGIIGDLGQTYDSN+TFEHY+
Sbjct: 121 TINNLEYDTKYFYELGSGNATRRFFFTTPPRVDPDVPYTFGIIGDLGQTYDSNQTFEHYY 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           +NPKGQAVLF GDLSYADNHP+HDNRKWDTW RFVEKSTAYQPWIWTAGNHE+DFAP+IG
Sbjct: 181 SNPKGQAVLFAGDLSYADNHPFHDNRKWDTWGRFVEKSTAYQPWIWTAGNHEIDFAPQIG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKPYTHRYHVPYKT+QS+SPLWYSIKRASAYIIVLSSYSAYG YTPQY+WL+ EF 
Sbjct: 241 ENTPFKPYTHRYHVPYKTAQSTSPLWYSIKRASAYIIVLSSYSAYGKYTPQYEWLQKEFK 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKRDETPWLIVMLH+PWYNSY+YHYMEGESMRVMFESWF+QNKVDLVLSGH+HAYERSE
Sbjct: 301 KVKRDETPWLIVMLHAPWYNSYNYHYMEGESMRVMFESWFIQNKVDLVLSGHIHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITN L +P+RD+NAPIYIT+GDGGNIEGLAN FTEPQPSYSA+REASFGHA+
Sbjct: 361 RVSNVRYNITNRLCSPVRDINAPIYITIGDGGNIEGLANEFTEPQPSYSAFREASFGHAI 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEA 460
           LEIKNRTHAYYTWHRN D+EPV+ADSLWIYNRYW+P+DE+
Sbjct: 421 LEIKNRTHAYYTWHRNHDNEPVSADSLWIYNRYWYPEDES 457

BLAST of MC01g_new0404 vs. NCBI nr
Match: XP_023518536.1 (purple acid phosphatase 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 847 bits (2189), Expect = 8.61e-309
Identity = 389/460 (84.57%), Postives = 426/460 (92.61%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAIS FI LLL    +S+A++CNGGITSK+ RK EASVDMP EAFPPP G+NAPEQVHI
Sbjct: 1   MAAISHFIVLLLS---ISLANLCNGGITSKFMRKLEASVDMPAEAFPPPAGYNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDRHGRG+IISWVTP SP P VVRYWA D D N++ KAHSRITTYKYYNYTS FIHHA
Sbjct: 61  TQGDRHGRGVIISWVTPLSPKPTVVRYWAADGDGNRRNKAHSRITTYKYYNYTSAFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TI+ LEYDTKYFYELGSGN  R F F TPP+ GPDVPYTFGIIGDLGQTYDSN+TFEHY+
Sbjct: 121 TINNLEYDTKYFYELGSGNATRRFFFTTPPRVGPDVPYTFGIIGDLGQTYDSNQTFEHYY 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           +NPKGQAVLF GDLSYADNHP+HDNRKWDTW RFVEKSTAYQPWIWTAGNHE+DFAP+IG
Sbjct: 181 SNPKGQAVLFAGDLSYADNHPFHDNRKWDTWGRFVEKSTAYQPWIWTAGNHEIDFAPQIG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKPYTHRYHVPYKT+QS+SPLWYSIKRASAYIIVLSSYSAYG YTPQY+WL+ EF 
Sbjct: 241 ENTPFKPYTHRYHVPYKTAQSTSPLWYSIKRASAYIIVLSSYSAYGKYTPQYEWLQKEFK 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKRDETPWLIVMLH+PWYNSY+YHYMEGESMRVMFESWF+QNKVD+VLSGH+HAYERSE
Sbjct: 301 KVKRDETPWLIVMLHAPWYNSYNYHYMEGESMRVMFESWFIQNKVDIVLSGHIHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITN L +P+RD+NAPIYIT+GDGGNIEGLAN FTEPQPSYSA+REASFGHA+
Sbjct: 361 RVSNVRYNITNRLCSPVRDINAPIYITIGDGGNIEGLANEFTEPQPSYSAFREASFGHAI 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEA 460
           LEIKNRTHAYYTWHRN D+EPV+ADSLWIYNRYW+P+DE+
Sbjct: 421 LEIKNRTHAYYTWHRNHDNEPVSADSLWIYNRYWYPEDES 457

BLAST of MC01g_new0404 vs. NCBI nr
Match: XP_023003385.1 (purple acid phosphatase 5-like [Cucurbita maxima])

HSP 1 Score: 847 bits (2188), Expect = 1.22e-308
Identity = 390/460 (84.78%), Postives = 426/460 (92.61%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAISQFI LLLL   +S+A++CNGGITSK+ RK EASVDMP  AFPPP G+NAPEQVHI
Sbjct: 1   MAAISQFIVLLLL---ISLANLCNGGITSKFMRKLEASVDMPAGAFPPPAGYNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDRHGRG+IISWVTP SP P VVRYWA D D N++ KAHSRITTYKYYNYTS FIHHA
Sbjct: 61  TQGDRHGRGVIISWVTPLSPKPTVVRYWAADGDGNRRNKAHSRITTYKYYNYTSAFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TI+ LEYDTKYFYELGSGN  R F F TPP+ GPDVPYTFGIIGDLGQTYDSN+T EHY+
Sbjct: 121 TINNLEYDTKYFYELGSGNATRQFFFTTPPRVGPDVPYTFGIIGDLGQTYDSNQTLEHYY 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           +NPKGQAVLF GDLSYADNHP+HDNRKWDTW RFVEKSTAYQPWIWTAGNHE+DFAP+IG
Sbjct: 181 SNPKGQAVLFAGDLSYADNHPFHDNRKWDTWGRFVEKSTAYQPWIWTAGNHEIDFAPQIG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKPYTHRYHVPYKT+QS+SPLWYSIKRASAYIIVLSSYSAYG YTPQY+WL+ EF 
Sbjct: 241 ENTPFKPYTHRYHVPYKTAQSTSPLWYSIKRASAYIIVLSSYSAYGKYTPQYEWLQKEFK 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKRDETPWLIVMLH+PWYNSY+YHYMEGESMRVMFESWF+QNKVD+VLSGH+HAYERSE
Sbjct: 301 KVKRDETPWLIVMLHAPWYNSYNYHYMEGESMRVMFESWFIQNKVDIVLSGHIHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITN L +P+RDVNAPIYIT+GDGGNIEGLAN FTEPQPSYSA+REASFGHA+
Sbjct: 361 RVSNVRYNITNRLCSPVRDVNAPIYITIGDGGNIEGLANEFTEPQPSYSAFREASFGHAI 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEA 460
           LEIKNRTHAYYTWHRN D+EPV+ADSLWIYNRYW+P+DE+
Sbjct: 421 LEIKNRTHAYYTWHRNHDNEPVSADSLWIYNRYWYPEDES 457

BLAST of MC01g_new0404 vs. ExPASy TrEMBL
Match: A0A6J1BSD7 (Purple acid phosphatase OS=Momordica charantia OX=3673 GN=LOC111005270 PE=3 SV=1)

HSP 1 Score: 978 bits (2529), Expect = 0.0
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI
Sbjct: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA
Sbjct: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF
Sbjct: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG
Sbjct: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL
Sbjct: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE
Sbjct: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM
Sbjct: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASSQ 463
           LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASSQ
Sbjct: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASSQ 463

BLAST of MC01g_new0404 vs. ExPASy TrEMBL
Match: A0A6J1HGV4 (Purple acid phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111463433 PE=3 SV=1)

HSP 1 Score: 847 bits (2189), Expect = 4.17e-309
Identity = 390/460 (84.78%), Postives = 426/460 (92.61%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAISQFI LLL    +S+A++CNGGITSK+ RK EASVDMP EAFPPP G+NAPEQVHI
Sbjct: 1   MAAISQFIVLLLS---ISLANLCNGGITSKFMRKLEASVDMPAEAFPPPAGYNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDRHGRG+IISWVTP SP P VVRYWA D D N++ KAHSRITTYKYYNYTS FIHHA
Sbjct: 61  TQGDRHGRGVIISWVTPLSPKPTVVRYWAADGDGNRRNKAHSRITTYKYYNYTSAFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TI+ LEYDTKYFYELGSGN  R F F TPP+  PDVPYTFGIIGDLGQTYDSN+TFEHY+
Sbjct: 121 TINNLEYDTKYFYELGSGNATRRFFFTTPPRVDPDVPYTFGIIGDLGQTYDSNQTFEHYY 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           +NPKGQAVLF GDLSYADNHP+HDNRKWDTW RFVEKSTAYQPWIWTAGNHE+DFAP+IG
Sbjct: 181 SNPKGQAVLFAGDLSYADNHPFHDNRKWDTWGRFVEKSTAYQPWIWTAGNHEIDFAPQIG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKPYTHRYHVPYKT+QS+SPLWYSIKRASAYIIVLSSYSAYG YTPQY+WL+ EF 
Sbjct: 241 ENTPFKPYTHRYHVPYKTAQSTSPLWYSIKRASAYIIVLSSYSAYGKYTPQYEWLQKEFK 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKRDETPWLIVMLH+PWYNSY+YHYMEGESMRVMFESWF+QNKVDLVLSGH+HAYERSE
Sbjct: 301 KVKRDETPWLIVMLHAPWYNSYNYHYMEGESMRVMFESWFIQNKVDLVLSGHIHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITN L +P+RD+NAPIYIT+GDGGNIEGLAN FTEPQPSYSA+REASFGHA+
Sbjct: 361 RVSNVRYNITNRLCSPVRDINAPIYITIGDGGNIEGLANEFTEPQPSYSAFREASFGHAI 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEA 460
           LEIKNRTHAYYTWHRN D+EPV+ADSLWIYNRYW+P+DE+
Sbjct: 421 LEIKNRTHAYYTWHRNHDNEPVSADSLWIYNRYWYPEDES 457

BLAST of MC01g_new0404 vs. ExPASy TrEMBL
Match: A0A6J1KRL7 (Purple acid phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111497010 PE=3 SV=1)

HSP 1 Score: 847 bits (2188), Expect = 5.92e-309
Identity = 390/460 (84.78%), Postives = 426/460 (92.61%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAISQFI LLLL   +S+A++CNGGITSK+ RK EASVDMP  AFPPP G+NAPEQVHI
Sbjct: 1   MAAISQFIVLLLL---ISLANLCNGGITSKFMRKLEASVDMPAGAFPPPAGYNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDRHGRG+IISWVTP SP P VVRYWA D D N++ KAHSRITTYKYYNYTS FIHHA
Sbjct: 61  TQGDRHGRGVIISWVTPLSPKPTVVRYWAADGDGNRRNKAHSRITTYKYYNYTSAFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TI+ LEYDTKYFYELGSGN  R F F TPP+ GPDVPYTFGIIGDLGQTYDSN+T EHY+
Sbjct: 121 TINNLEYDTKYFYELGSGNATRQFFFTTPPRVGPDVPYTFGIIGDLGQTYDSNQTLEHYY 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           +NPKGQAVLF GDLSYADNHP+HDNRKWDTW RFVEKSTAYQPWIWTAGNHE+DFAP+IG
Sbjct: 181 SNPKGQAVLFAGDLSYADNHPFHDNRKWDTWGRFVEKSTAYQPWIWTAGNHEIDFAPQIG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKPYTHRYHVPYKT+QS+SPLWYSIKRASAYIIVLSSYSAYG YTPQY+WL+ EF 
Sbjct: 241 ENTPFKPYTHRYHVPYKTAQSTSPLWYSIKRASAYIIVLSSYSAYGKYTPQYEWLQKEFK 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKRDETPWLIVMLH+PWYNSY+YHYMEGESMRVMFESWF+QNKVD+VLSGH+HAYERSE
Sbjct: 301 KVKRDETPWLIVMLHAPWYNSYNYHYMEGESMRVMFESWFIQNKVDIVLSGHIHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITN L +P+RDVNAPIYIT+GDGGNIEGLAN FTEPQPSYSA+REASFGHA+
Sbjct: 361 RVSNVRYNITNRLCSPVRDVNAPIYITIGDGGNIEGLANEFTEPQPSYSAFREASFGHAI 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEA 460
           LEIKNRTHAYYTWHRN D+EPV+ADSLWIYNRYW+P+DE+
Sbjct: 421 LEIKNRTHAYYTWHRNHDNEPVSADSLWIYNRYWYPEDES 457

BLAST of MC01g_new0404 vs. ExPASy TrEMBL
Match: A0A5D3CMB9 (Purple acid phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001620 PE=3 SV=1)

HSP 1 Score: 842 bits (2174), Expect = 8.04e-307
Identity = 392/461 (85.03%), Postives = 427/461 (92.62%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAI+Q IALLL    LS+A + +GGITS++ RK EASVDMP EAFPPP G NAPEQVHI
Sbjct: 1   MAAIAQSIALLLF---LSLAYLSHGGITSRFMRKLEASVDMPAEAFPPPSGHNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDR+GRG+IISWVTP SP PNVVRYWA DSDE+      SRITTYKYYNYTSGFIHHA
Sbjct: 61  TQGDRNGRGVIISWVTPLSPKPNVVRYWAADSDEDHDHTVRSRITTYKYYNYTSGFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TI +LEYDTKYFYE+GSG+ KR F F TPP  GPDVPYTFGIIGDLGQTYDSN+TFEHY+
Sbjct: 121 TISDLEYDTKYFYEIGSGDAKRRFFFTTPPMVGPDVPYTFGIIGDLGQTYDSNQTFEHYY 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           +N KGQAVLFVGDLSYADNHP+HDNRKWDTW RFVEKSTAYQPWIWTAGNHEMDFAPE+G
Sbjct: 181 SNSKGQAVLFVGDLSYADNHPFHDNRKWDTWGRFVEKSTAYQPWIWTAGNHEMDFAPELG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKP+THRYHVPYK++QSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWL+NEF 
Sbjct: 241 ENTPFKPFTHRYHVPYKSAQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLKNEFT 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKR+ETPWLIVMLHSPWYNSYDYHYMEGESMRVMFE WFV+NKVDLVLSGHVHAYERSE
Sbjct: 301 KVKREETPWLIVMLHSPWYNSYDYHYMEGESMRVMFEPWFVENKVDLVLSGHVHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITN LSTPIRD+NAP+Y+T+GDGGNIEGLAN+FTEPQP+YSA+REASFGHA+
Sbjct: 361 RVSNVRYNITNRLSTPIRDINAPMYVTIGDGGNIEGLANQFTEPQPNYSAFREASFGHAL 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEAS 461
           LEIKNRTHAYYTWHRN D+EPVAADSLWIYNRYW+P+DE+S
Sbjct: 421 LEIKNRTHAYYTWHRNHDNEPVAADSLWIYNRYWYPEDESS 458

BLAST of MC01g_new0404 vs. ExPASy TrEMBL
Match: A0A1S3B1Z1 (Purple acid phosphatase OS=Cucumis melo OX=3656 GN=LOC103485096 PE=3 SV=1)

HSP 1 Score: 842 bits (2174), Expect = 8.04e-307
Identity = 392/461 (85.03%), Postives = 427/461 (92.62%), Query Frame = 0

Query: 1   MAAISQFIALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHI 60
           MAAI+Q IALLL    LS+A + +GGITS++ RK EASVDMP EAFPPP G NAPEQVHI
Sbjct: 1   MAAIAQSIALLLF---LSLAYLSHGGITSRFMRKLEASVDMPAEAFPPPSGHNAPEQVHI 60

Query: 61  TQGDRHGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHA 120
           TQGDR+GRG+IISWVTP SP PNVVRYWA DSDE+      SRITTYKYYNYTSGFIHHA
Sbjct: 61  TQGDRNGRGVIISWVTPLSPKPNVVRYWAADSDEDHDHTVRSRITTYKYYNYTSGFIHHA 120

Query: 121 TIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYF 180
           TI +LEYDTKYFYE+GSG+ KR F F TPP  GPDVPYTFGIIGDLGQTYDSN+TFEHY+
Sbjct: 121 TISDLEYDTKYFYEIGSGDAKRRFFFTTPPMVGPDVPYTFGIIGDLGQTYDSNQTFEHYY 180

Query: 181 TNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIG 240
           +N KGQAVLFVGDLSYADNHP+HDNRKWDTW RFVEKSTAYQPWIWTAGNHEMDFAPE+G
Sbjct: 181 SNSKGQAVLFVGDLSYADNHPFHDNRKWDTWGRFVEKSTAYQPWIWTAGNHEMDFAPELG 240

Query: 241 ENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFL 300
           ENTPFKP+THRYHVPYK++QSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWL+NEF 
Sbjct: 241 ENTPFKPFTHRYHVPYKSAQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLKNEFT 300

Query: 301 KVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSE 360
           KVKR+ETPWLIVMLHSPWYNSYDYHYMEGESMRVMFE WFV+NKVDLVLSGHVHAYERSE
Sbjct: 301 KVKREETPWLIVMLHSPWYNSYDYHYMEGESMRVMFEPWFVENKVDLVLSGHVHAYERSE 360

Query: 361 RVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAM 420
           RVSNVRYNITN LSTPIRD+NAP+Y+T+GDGGNIEGLAN+FTEPQP+YSA+REASFGHA+
Sbjct: 361 RVSNVRYNITNRLSTPIRDINAPMYVTIGDGGNIEGLANQFTEPQPNYSAFREASFGHAL 420

Query: 421 LEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEAS 461
           LEIKNRTHAYYTWHRN D+EPVAADSLWIYNRYW+P+DE+S
Sbjct: 421 LEIKNRTHAYYTWHRNHDNEPVAADSLWIYNRYWYPEDESS 458

BLAST of MC01g_new0404 vs. TAIR 10
Match: AT2G16430.2 (purple acid phosphatase 10 )

HSP 1 Score: 678.3 bits (1749), Expect = 4.3e-195
Identity = 310/458 (67.69%), Postives = 369/458 (80.57%), Query Frame = 0

Query: 8   IALLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEA--FPPPPGFNAPEQVHITQGDR 67
           I L+L  +L S+  +CNGGITS+Y RK EA+VDMP+++  F  P G+NAP+QVHITQGD 
Sbjct: 12  IVLVLCCVLNSL--LCNGGITSRYVRKLEATVDMPLDSDVFRVPCGYNAPQQVHITQGDV 71

Query: 68  HGRGIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHATIDEL 127
            G+ +I+SWVT  +   N V YW E+S   +K KAH +  TYK+YNYTSGFIHH  I  L
Sbjct: 72  EGKAVIVSWVTQEAKGSNKVIYWKENS--TKKHKAHGKTNTYKFYNYTSGFIHHCPIRNL 131

Query: 128 EYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNP-K 187
           EYDTKY+Y LG G T+R F F TPP+ GPDVPYTFG+IGDLGQ+YDSN T  HY  NP K
Sbjct: 132 EYDTKYYYVLGVGQTERKFWFFTPPEIGPDVPYTFGLIGDLGQSYDSNITLTHYENNPTK 191

Query: 188 GQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTP 247
           GQAVLFVGD+SYAD +P HDNR+WD+W RF E+STAYQPWIWT GNHE+DFAPEIGEN P
Sbjct: 192 GQAVLFVGDISYADTYPDHDNRRWDSWGRFAERSTAYQPWIWTTGNHELDFAPEIGENRP 251

Query: 248 FKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKR 307
           FKP+THRY  PY++S S+ P WYSIKR  AYIIVL+SYSAYG YTPQYQWLE EF KV R
Sbjct: 252 FKPFTHRYRTPYRSSGSTEPFWYSIKRGPAYIIVLASYSAYGKYTPQYQWLEEEFPKVNR 311

Query: 308 DETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSN 367
            ETPWLIV++HSPWYNSYDYHYMEGE+MRVM+E+WFV+ KVD+V +GHVHAYERSERVSN
Sbjct: 312 TETPWLIVLMHSPWYNSYDYHYMEGETMRVMYEAWFVKYKVDVVFAGHVHAYERSERVSN 371

Query: 368 VRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIK 427
           + YN+ NG+ TP++D +AP+YIT+GDGGNIEGLA + TEPQP YSA+REASFGHA+  IK
Sbjct: 372 IAYNVVNGICTPVKDQSAPVYITIGDGGNIEGLATKMTEPQPKYSAFREASFGHAIFSIK 431

Query: 428 NRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASS 463
           NRTHA+Y WHRN D   V  D +W YNR+W P D++ S
Sbjct: 432 NRTHAHYGWHRNHDGYAVEGDRMWFYNRFWHPVDDSPS 465

BLAST of MC01g_new0404 vs. TAIR 10
Match: AT4G36350.1 (purple acid phosphatase 25 )

HSP 1 Score: 674.5 bits (1739), Expect = 6.2e-194
Identity = 317/456 (69.52%), Postives = 373/456 (81.80%), Query Frame = 0

Query: 10  LLLLALLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHITQGDRHGRG 69
           +LL+ + LS+A V N G TS + R  + S +M +E FP P G NAPEQVHI QGD +GRG
Sbjct: 6   ILLVFVFLSIATVINSGTTSNFVRTAQPSTEMSLETFPSPAGHNAPEQVHIVQGDYNGRG 65

Query: 70  IIISWVTPRS-PHPNVVRYW-AEDSD-ENQKQKAHSRITTYKYYNYTSGFIHHATIDELE 129
           IIISWVTP +    NVV YW A D D + +K++ H+  ++Y++Y+YTSGF+HHATI  LE
Sbjct: 66  IIISWVTPLNLAGSNVVTYWKAVDGDVKPKKKRGHASTSSYRFYDYTSGFLHHATIKGLE 125

Query: 130 YDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNPKGQ 189
           YDTKY YE+G+  + R FSF +PPK GPDVPYTFGIIGDLGQT  SN+T  HY +NPKGQ
Sbjct: 126 YDTKYIYEVGTDGSVRQFSFTSPPKVGPDVPYTFGIIGDLGQTLASNETLYHYMSNPKGQ 185

Query: 190 AVLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTPFK 249
           AVLF GDLSYAD+HP HD RKWD+W RFVE   AYQ +I+ AGNHE+DF P IGE   FK
Sbjct: 186 AVLFPGDLSYADDHPNHDQRKWDSWGRFVEPCAAYQTFIYAAGNHEIDFVPNIGEPHAFK 245

Query: 250 PYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKRDE 309
           PY HRYH  YK S+S SPLWYSI+RASA+IIVLSSYSAYG YTPQY WLE E  KV R+E
Sbjct: 246 PYIHRYHNAYKASKSISPLWYSIRRASAHIIVLSSYSAYGKYTPQYVWLEQELKKVNREE 305

Query: 310 TPWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSNVR 369
           TPWLIVM+HSPWYNS +YHYMEGESMR MFESWFV +KVDLVLSGHVH+YERSERVSN++
Sbjct: 306 TPWLIVMVHSPWYNSNNYHYMEGESMRAMFESWFVNSKVDLVLSGHVHSYERSERVSNIK 365

Query: 370 YNITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIKNR 429
           YNITNGLS P++D +APIYIT+GDGGNIEG+AN FT+PQPSYSAYREASFGHA+LEI NR
Sbjct: 366 YNITNGLSYPVKDPSAPIYITIGDGGNIEGIANSFTDPQPSYSAYREASFGHAVLEIYNR 425

Query: 430 THAYYTWHRNEDHEPVAADSLWIYNRYWFPQDEASS 463
           THAYYTWHRN+D+EPVAADS+ ++NRY+FP +E  S
Sbjct: 426 THAYYTWHRNQDNEPVAADSIMLHNRYFFPVEELES 461

BLAST of MC01g_new0404 vs. TAIR 10
Match: AT1G56360.1 (purple acid phosphatase 6 )

HSP 1 Score: 673.3 bits (1736), Expect = 1.4e-193
Identity = 310/452 (68.58%), Postives = 370/452 (81.86%), Query Frame = 0

Query: 15  LLLSVADVCNGGITSKYTRKPEASVDMPVEAFPPPPGFNAPEQVHITQGDRHGRGIIISW 74
           L LS+  V NGGITSK+ R+   S++M ++ FP P G+N PEQVH+TQGD  GRG+I+SW
Sbjct: 10  LFLSITTVINGGITSKFVRQALPSIEMSLDTFPSPGGYNTPEQVHLTQGDHDGRGMIVSW 69

Query: 75  VTPRS-PHPNVVRYWAEDSDEN---QKQKAHSRITTYKYYNYTSGFIHHATIDELEYDTK 134
           VTP +    NVV YW   +  +    K++AH+   +Y++Y+Y+SGF+HHATI  LEYDTK
Sbjct: 70  VTPLNLAGSNVVTYWIATNGSDVKPAKKRAHASTKSYRFYDYSSGFLHHATIKGLEYDTK 129

Query: 135 YFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNPKGQAVLF 194
           Y YE+G+  + R FSF TPPK GPDVPYTFGIIGDLGQTY SN+T  HY +NPKGQAVLF
Sbjct: 130 YIYEVGTDKSVRQFSFTTPPKIGPDVPYTFGIIGDLGQTYASNETLYHYMSNPKGQAVLF 189

Query: 195 VGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTPFKPYTH 254
            GDLSYAD+HP HD RKWDTW RF+E   AYQP+I+ AGNHE+DF P IGE   FKPYTH
Sbjct: 190 AGDLSYADDHPNHDQRKWDTWGRFMEPCAAYQPFIFAAGNHEIDFVPNIGEPHAFKPYTH 249

Query: 255 RYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKRDETPWL 314
           RY   YK SQS+SPLWYS++RASA+IIVLSSYSAYG YTPQY WLE E   V R+ETPWL
Sbjct: 250 RYPNAYKASQSTSPLWYSVRRASAHIIVLSSYSAYGKYTPQYIWLEQELKNVNREETPWL 309

Query: 315 IVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSNVRYNIT 374
           IV++HSPWYNS +YHYMEGESMRVMFESW V +KVDLVLSGHVHAYERSER+SN++YNIT
Sbjct: 310 IVIVHSPWYNSNNYHYMEGESMRVMFESWLVNSKVDLVLSGHVHAYERSERISNIKYNIT 369

Query: 375 NGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIKNRTHAY 434
           NGLS+P++D NAPIYIT+GDGGNIEG+AN F +PQPSYSAYREASFGHA+LEI NRTHA 
Sbjct: 370 NGLSSPVKDPNAPIYITIGDGGNIEGIANSFVDPQPSYSAYREASFGHAVLEIMNRTHAQ 429

Query: 435 YTWHRNEDHEPVAADSLWIYNRYWFPQDEASS 463
           YTWHRN+D+EPVAADS+ ++NR++FP +E  S
Sbjct: 430 YTWHRNQDNEPVAADSIMLHNRHFFPVEEIVS 461

BLAST of MC01g_new0404 vs. TAIR 10
Match: AT2G27190.1 (purple acid phosphatase 12 )

HSP 1 Score: 636.3 bits (1640), Expect = 1.9e-182
Identity = 289/450 (64.22%), Postives = 354/450 (78.67%), Query Frame = 0

Query: 11  LLLALLLSVADVCNGGITSKYTRKPEASVDMPVEA--FPPPPGFNAPEQVHITQGDRHGR 70
           L++ LL  + + C GG TS+Y R  +   DMP+++  F  PPG N+P+QVH+TQG+  G 
Sbjct: 14  LIIFLLSVLVEFCYGGFTSEYVRGSDLPDDMPLDSDVFEVPPGPNSPQQVHVTQGNHEGN 73

Query: 71  GIIISWVTPRSPHPNVVRYWAEDSDENQKQKAHSRITTYKYYNYTSGFIHHATIDELEYD 130
           G+IISWVTP  P    V+YW E  +E  +++A + + TY+++NYTSG+IHH  ID+LE+D
Sbjct: 74  GVIISWVTPVKPGSKTVQYWCE--NEKSRKQAEATVNTYRFFNYTSGYIHHCLIDDLEFD 133

Query: 131 TKYFYELGSGNTKRGFSFRTPPKPGPDVPYTFGIIGDLGQTYDSNKTFEHYFTNP-KGQA 190
           TKY+YE+GSG   R F F  PPK GPDVPYTFG+IGDLGQTYDSN T  HY  NP KGQA
Sbjct: 134 TKYYYEIGSGKWSRRFWFFIPPKSGPDVPYTFGLIGDLGQTYDSNSTLSHYEMNPGKGQA 193

Query: 191 VLFVGDLSYADNHPYHDNRKWDTWARFVEKSTAYQPWIWTAGNHEMDFAPEIGENTPFKP 250
           VLFVGDLSYAD +P HDN +WDTW RFVE+S AYQPWIWTAGNHE+DF P+IGE  PFKP
Sbjct: 194 VLFVGDLSYADRYPNHDNNRWDTWGRFVERSVAYQPWIWTAGNHEIDFVPDIGEIEPFKP 253

Query: 251 YTHRYHVPYKTSQSSSPLWYSIKRASAYIIVLSSYSAYGTYTPQYQWLENEFLKVKRDET 310
           + +RYH P+K S S SPLWYSIKRASAYIIV+S YS+YG YTPQY+WLE E   V R ET
Sbjct: 254 FMNRYHTPHKASGSISPLWYSIKRASAYIIVMSCYSSYGIYTPQYKWLEKELQGVNRTET 313

Query: 311 PWLIVMLHSPWYNSYDYHYMEGESMRVMFESWFVQNKVDLVLSGHVHAYERSERVSNVRY 370
           PWLIV++HSP+Y+SY +HYMEGE++RVM+E WFV+ KVD+V +GHVHAYERSERVSN+ Y
Sbjct: 314 PWLIVLVHSPFYSSYVHHYMEGETLRVMYEQWFVKYKVDVVFAGHVHAYERSERVSNIAY 373

Query: 371 NITNGLSTPIRDVNAPIYITVGDGGNIEGLANRFTEPQPSYSAYREASFGHAMLEIKNRT 430
           NI NGL  PI D +APIYIT+GDGGN EGL     +PQP YSA+REASFGH +LEIKNRT
Sbjct: 374 NIVNGLCEPISDESAPIYITIGDGGNSEGLLTDMMQPQPKYSAFREASFGHGLLEIKNRT 433

Query: 431 HAYYTWHRNEDHEPVAADSLWIYNRYWFPQ 458
           HAY++W+RN+D   VAADS+W+ NR+W  Q
Sbjct: 434 HAYFSWNRNQDGNAVAADSVWLLNRFWRAQ 461

BLAST of MC01g_new0404 vs. TAIR 10
Match: AT1G52940.1 (purple acid phosphatase 5 )

HSP 1 Score: 595.9 bits (1535), Expect = 2.8e-170
Identity = 270/420 (64.29%), Postives = 328/420 (78.10%), Query Frame = 0

Query: 41  MPVEAFPPPPGFNAPEQVHITQGDRHGRGIIISWVTP-RSPHPNVVRYWAEDSDENQKQK 100
           M +E FPPP G+NAPEQVHITQGD +GRG+IISWVT       NVV YW   SD +  + 
Sbjct: 1   MSLETFPPPAGYNAPEQVHITQGDHNGRGMIISWVTSLNEDGSNVVTYWIASSDGSDNKS 60

Query: 101 AHSRITTYKYYNYTSGFIHHATIDELEYDTKYFYELGSGNTKRGFSFRTPPKPGPDVPYT 160
             +  ++Y+Y++YTSG++HHA I ELEY TKYFYELG+G + R F+  TPPK GPDVPYT
Sbjct: 61  VIATTSSYRYFDYTSGYLHHAIIKELEYKTKYFYELGTGRSTRQFNL-TPPKVGPDVPYT 120

Query: 161 FGIIGDLGQTYDSNKTFEHYFTNPKGQAVLFVGDLSYADNHPYHDNRKWDTWARFVEKST 220
           FG+IGDLGQTY SN+T  +Y +NPKGQAVLF GDLSYAD+HP HD  KWD++ RFVE S 
Sbjct: 121 FGVIGDLGQTYASNQTLYNYMSNPKGQAVLFAGDLSYADDHPNHDQSKWDSYGRFVEPSA 180

Query: 221 AYQPWIWTAGNHEMDFAPEIGENTPFKPYTHRYHVPYKTSQSSSPLWYSIKRASAYIIVL 280
           AYQPWIW AGNHE+D+A  IGE  PFKPY +RYHVPY+ SQ+                  
Sbjct: 181 AYQPWIWAAGNHEIDYAQSIGETQPFKPYKNRYHVPYRASQNK----------------- 240

Query: 281 SSYSAYGTYTPQYQWLENEFLKVKRDETPWLIVMLHSPWYNSYDYHYMEGESMRVMFESW 340
                   YTPQ  WL++EF KV R ETPWLIV++H+PWYNS +YHYMEGESMRV FE W
Sbjct: 241 --------YTPQNSWLQDEFKKVNRSETPWLIVLVHAPWYNSNNYHYMEGESMRVTFEPW 300

Query: 341 FVQNKVDLVLSGHVHAYERSERVSNVRYNITNGLSTPIRDVNAPIYITVGDGGNIEGLAN 400
           FV+NKVD+V +GHVHAYERSERVSN++YNIT+G+STP++D NAP+YIT+GDGGNIEG+AN
Sbjct: 301 FVENKVDIVFAGHVHAYERSERVSNIQYNITDGMSTPVKDQNAPVYITIGDGGNIEGIAN 360

Query: 401 RFTEPQPSYSAYREASFGHAMLEIKNRTHAYYTWHRNEDHEPVAADSLWIYNRYWFPQDE 460
            FT+PQPSYSA+REASFGHA+LEIKNRTHA+YTWHRN++ E V ADS+W+ NRY+ P++E
Sbjct: 361 IFTDPQPSYSAFREASFGHALLEIKNRTHAHYTWHRNKEDEAVIADSIWLKNRYYLPEEE 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q091313.5e-19467.98Purple acid phosphatase OS=Glycine max OX=3847 GN=PAP PE=1 SV=2[more]
Q9SIV96.0e-19467.69Purple acid phosphatase 10 OS=Arabidopsis thaliana OX=3702 GN=PAP10 PE=2 SV=1[more]
O232448.7e-19369.52Purple acid phosphatase 25 OS=Arabidopsis thaliana OX=3702 GN=PAP25 PE=2 SV=2[more]
Q9C5101.9e-19268.58Purple acid phosphatase 6 OS=Arabidopsis thaliana OX=3702 GN=PAP6 PE=2 SV=1[more]
Q9SDZ92.5e-19267.91Purple acid phosphatase 2 OS=Ipomoea batatas OX=4120 GN=PAP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022132405.10.0100.00purple acid phosphatase 5 [Momordica charantia][more]
KAG7026765.12.58e-31085.00Purple acid phosphatase 6 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022963133.18.61e-30984.78purple acid phosphatase 5-like [Cucurbita moschata][more]
XP_023518536.18.61e-30984.57purple acid phosphatase 5-like [Cucurbita pepo subsp. pepo][more]
XP_023003385.11.22e-30884.78purple acid phosphatase 5-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1BSD70.0100.00Purple acid phosphatase OS=Momordica charantia OX=3673 GN=LOC111005270 PE=3 SV=1[more]
A0A6J1HGV44.17e-30984.78Purple acid phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111463433 PE=3 SV=1[more]
A0A6J1KRL75.92e-30984.78Purple acid phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111497010 PE=3 SV=1[more]
A0A5D3CMB98.04e-30785.03Purple acid phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B1Z18.04e-30785.03Purple acid phosphatase OS=Cucumis melo OX=3656 GN=LOC103485096 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G16430.24.3e-19567.69purple acid phosphatase 10 [more]
AT4G36350.16.2e-19469.52purple acid phosphatase 25 [more]
AT1G56360.11.4e-19368.58purple acid phosphatase 6 [more]
AT2G27190.11.9e-18264.22purple acid phosphatase 12 [more]
AT1G52940.12.8e-17064.29purple acid phosphatase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025733Iron/zinc purple acid phosphatase-like C-terminal domainPFAMPF14008Metallophos_Ccoord: 382..439
e-value: 1.7E-18
score: 67.2
IPR015914Purple acid phosphatase, N-terminalPFAMPF16656Pur_ac_phosph_Ncoord: 55..148
e-value: 2.1E-19
score: 69.9
NoneNo IPR availableGENE3D2.60.40.380coord: 54..152
e-value: 3.3E-32
score: 112.6
NoneNo IPR availablePANTHERPTHR22953:SF120PURPLE ACID PHOSPHATASE 11-RELATEDcoord: 23..460
IPR029052Metallo-dependent phosphatase-likeGENE3D3.60.21.10coord: 153..461
e-value: 3.1E-123
score: 413.3
IPR029052Metallo-dependent phosphatase-likeSUPERFAMILY56300Metallo-dependent phosphatasescoord: 152..457
IPR004843Calcineurin-like phosphoesterase domain, ApaH typePFAMPF00149Metallophoscoord: 159..356
e-value: 6.3E-26
score: 92.1
IPR039331Purple acid phosphatase-likePANTHERPTHR22953ACID PHOSPHATASE RELATEDcoord: 23..460
IPR041792Purple acid phosphatase, metallophosphatase domainCDDcd00839MPP_PAPscoord: 154..451
e-value: 6.47013E-105
score: 312.311
IPR008963Purple acid phosphatase-like, N-terminalSUPERFAMILY49363Purple acid phosphatase, N-terminal domaincoord: 42..150

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g_new0404.1MC01g_new0404.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
molecular_function GO:0003993 acid phosphatase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016787 hydrolase activity