MC01g_new0386 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0386 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.2e-99
Identity = 191/371 (51.48%), Postives = 244/371 (65.77%), Query Frame = 0

Query: 2   RKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKS-GVPVDPTSKIFALNLDMTCLM 61
           RKMCT+ELL++HK+ SFKSMR EE+ L +E +R AA + GV VD + K+ +L++DM+C M
Sbjct: 124 RKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVDMSCRM 183

Query: 62  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 121
           + G+KY  EE D ERGFK VVREA++L +APNLGD I  IA  DLQG  +R KSV K  D
Sbjct: 184 VLGKKYRDEEFD-ERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKSVNKAFD 243

Query: 122 GFLESIIDEHLNEANSD-TKDFVDVMLDIVGSRETEYQIDRSNIKAIILRQLTP------ 181
              E II+EHL   + + T DFVDVM+  +GS E+EY+I+R +IKAI+L  L        
Sbjct: 244 NLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLVASMDTSA 303

Query: 182 --------------------RPPLSNGHCLS-----------------LSNTPMLHPPGP 241
                               +  L N   L                  +  T  LHP  P
Sbjct: 304 TTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETFRLHPVAP 363

Query: 242 LLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKD 301
           LL+PH S ED T+N ++IPKKSRV+++  AIGRDP+ W DAEKF PERF GS +DV+G  
Sbjct: 364 LLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSSVDVRGNH 423

Query: 302 FQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRA 328
           FQLIPFG+GRR CPG++LGLTVV +V+AQLVHCFDW+LPN+MLP ELDM+E+ GLT  RA
Sbjct: 424 FQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCFDWELPNNMLPEELDMTEEFGLTVPRA 483

BLAST of MC01g_new0386 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 7.8e-71
Identity = 156/378 (41.27%), Postives = 210/378 (55.56%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           +RK   LELL   K+ SF  MR EELG +++ ++EA+ +   VD ++K+  +  +MT  +
Sbjct: 128 IRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRL 187

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           + GR       D     K ++ EAL L    N+ D +P +   D+QGL R+ K  GK +D
Sbjct: 188 LLGR-----TKDDRYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLD 247

Query: 121 GFLESIIDEH-LNEANSDTK-DFVDVMLDIVGSRETEYQ-----IDRSNIKAI------- 180
             LE IIDEH  N +N +   DF+D ML +       +      IDRS IKAI       
Sbjct: 248 KILEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISA 307

Query: 181 -----------ILRQLTPRP-------------------------PLSNGHCLSLSNTPM 240
                      IL +L   P                         P      + +     
Sbjct: 308 AIDTSDTSIEWILTELIKHPRAMKKCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLR 367

Query: 241 LHPPGPLLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWID-AEKFFPERFIGSQ 300
           LHP  PLL PHES ED TIN ++IPK+SRVIV++ A+GRDP+VW + AE+F PERF GS 
Sbjct: 368 LHPVAPLLGPHESMEDITINGYFIPKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSN 427

Query: 301 LDVKGKDFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKS 328
           +DV+G+DFQL+PFG+GRR CPGM+LGL  V +V+A+LVHCFDW LPN   P  LDM+EK 
Sbjct: 428 VDVRGRDFQLLPFGSGRRGCPGMQLGLITVQLVVARLVHCFDWNLPNGTTPDNLDMTEKF 487

BLAST of MC01g_new0386 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 134/372 (36.02%), Postives = 202/372 (54.30%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           +RK+C LELL+  +V S++S+R EE+GL++E + ++  +G  V+ +  +  L+      +
Sbjct: 126 VRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRV 185

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFD-LQGLNRRAKSVGKVI 120
            FG+KY GEE + +  F  +  E   L  A  +GD  P  A  D L G++ R K     +
Sbjct: 186 AFGKKYEGEE-ERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGEL 245

Query: 121 DGFLESIIDEHL--NEANS----DTKDFVDVMLDIVGSRETEYQIDRSNIKAIIL----- 180
           D F++ +ID+HL   +AN     + KD VDV+L +         ++R+N+KA+IL     
Sbjct: 246 DAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSG 305

Query: 181 -------------RQLTPRP-----------------------PLSNGHCLSL--SNTPM 240
                         +L   P                        L   H L L    T  
Sbjct: 306 GTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLR 365

Query: 241 LHPPGPLLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQL 300
           LHP  PLLVP ES+ D  I  ++IP K+RV ++A AIGRDP  W +AE+F PERF+ + +
Sbjct: 366 LHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSV 425

Query: 301 DVKGKDFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSG 323
           D KG+DFQLIPFGAGRR CPG+  G++ V + +A L++ F+W+LP D+   +LDMSE  G
Sbjct: 426 DFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAVG 485

BLAST of MC01g_new0386 vs. ExPASy Swiss-Prot
Match: O48923 (Cytochrome P450 71D10 OS=Glycine max OX=3847 GN=CYP71D10 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 7.1e-56
Identity = 131/377 (34.75%), Postives = 197/377 (52.25%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAA--KSGVPVDPTSKIFALNLDMTC 60
           +RK+CT+ELL + +VQSF+S+R EE+  L++ +   A  + G   + T  I+++   +  
Sbjct: 140 LRKICTVELLTAKRVQSFRSIREEEVAELVKKIAATASEEGGSIFNLTQSIYSMTFGIAA 199

Query: 61  LMIFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKV 120
              FG+K   ++V +       + + L L    ++ DL P    F + G   + + V +V
Sbjct: 200 RAAFGKKSRYQQVFISN-----MHKQLMLLGGFSVADLYPSSRVFQMMGATGKLEKVHRV 259

Query: 121 IDGFLESIIDEHLN-----EANSDTKDFVDVMLDIVGSRETEYQIDRSNIKAII------ 180
            D  L+ IIDEH N     E     +D VDV+L     +E+E+++   NIKA+I      
Sbjct: 260 TDRVLQDIIDEHKNRNRSSEEREAVEDLVDVLLKF--QKESEFRLTDDNIKAVIQDIFIG 319

Query: 181 ------------LRQLTPRPPL-------------SNGHC------------LSLSNTPM 240
                       + +L   P +             S G+               +  T  
Sbjct: 320 GGETSSSVVEWGMSELIRNPRVMEEAQAEVRRVYDSKGYVDETELHQLIYLKSIIKETMR 379

Query: 241 LHPPGPLLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQL 300
           LHPP PLLVP  S E   IN + IP K+R+I++A AIGR+P  W + E F PERF+ S +
Sbjct: 380 LHPPVPLLVPRVSRERCQINGYEIPSKTRIIINAWAIGRNPKYWGETESFKPERFLNSSI 439

Query: 301 DVKGKDFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSG 328
           D +G DF+ IPFGAGRR CPG+   +  + + +AQL++ FDWKLPN M   ELDM+E +G
Sbjct: 440 DFRGTDFEFIPFGAGRRICPGITFAIPNIELPLAQLLYHFDWKLPNKMKNEELDMTESNG 499

BLAST of MC01g_new0386 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-55
Identity = 132/369 (35.77%), Postives = 188/369 (50.95%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           +RK+C LELL++ +VQSF  +R EE+  L+  + E       +     ++A   D+ C +
Sbjct: 143 IRKICILELLSAKRVQSFSHVREEEVARLVRRVAEFYPGTTNLTKMLGLYA--NDVLCRV 202

Query: 61  IFGRKYVGEEVDVER-GFKWVVREALRLGAAPNLGDLIPCIASF-DLQGLNRRAKSVGKV 120
            FGR +  E  D +R GF+ ++ E   L    ++GD  P +     L G+  R +   + 
Sbjct: 203 AFGRGF-SEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETFRR 262

Query: 121 IDGFLESIIDEHLN--EANSDTKDFVDVMLDIVGSRETEYQIDRSNIKAIIL-------- 180
            D   + ++ +HL+      + KD VDV+LDI     TE  +   N+KAIIL        
Sbjct: 263 FDELFDQMVTDHLSPKREKEEHKDLVDVLLDIQKKESTEMPLTMDNVKAIILDMFAAGTD 322

Query: 181 ----------------------RQLTPRPPLSNGHCLSLSNTPM-------------LHP 240
                                  Q   R  +     +  S+ P              LHP
Sbjct: 323 TTFITLDWGMTELLMNRKVLERAQAEVRGVVGERRVVLESDLPQLDYMKAVIKEIFRLHP 382

Query: 241 PGPLLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVK 300
           P P+LVP ES ED TI+ + I  K+R+ V+A AIGRDP  W D E F PERFIGS +D K
Sbjct: 383 PAPVLVPRESMEDVTIDGYDILAKTRIFVNAWAIGRDPESWEDPEAFEPERFIGSTIDFK 442

Query: 301 GKDFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTF 323
           G+DF+LIPFGAGRR CP +  G   + + +AQL+H FDW+LP D    +LDM+E  G+T 
Sbjct: 443 GQDFELIPFGAGRRGCPAVTFGTATIELALAQLLHTFDWELPLDTAAKDLDMTEVFGITM 502

BLAST of MC01g_new0386 vs. NCBI nr
Match: XP_022132586.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 526 bits (1356), Expect = 1.04e-183
Identity = 278/372 (74.73%), Postives = 298/372 (80.11%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRKMCTLELLNSHKVQSFKSMR EE+GLL EDLREAAK+GV VD TSKIF+LNLDMTCLM
Sbjct: 127 MRKMCTLELLNSHKVQSFKSMRREEVGLLTEDLREAAKTGVAVDLTSKIFSLNLDMTCLM 186

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           IFGRKYVG+EVD ERGFKWVV+EALRLGAAPNLGDLIPCIA FDLQGLNRRAKSVGKV+D
Sbjct: 187 IFGRKYVGKEVDDERGFKWVVQEALRLGAAPNLGDLIPCIAPFDLQGLNRRAKSVGKVLD 246

Query: 121 GFLESIIDEHLNEANSDTKDFVDVMLDIVGSRETEYQIDRSNIKAIIL------------ 180
           GFLESIIDEHL EA+++TKDFVDVMLDIVGSRETEYQIDRSNIKAIIL            
Sbjct: 247 GFLESIIDEHLREADNETKDFVDVMLDIVGSRETEYQIDRSNIKAIILDMVLATTDTTAT 306

Query: 181 ------RQLTPRPPLSNG------HCLSLSNTP-------------------MLHPPGPL 240
                  +L   P +           + LS                       LHPPGPL
Sbjct: 307 TIEWALSELVKHPRVMKKLQEELEKVVGLSGMVEESDLDYLKYLDMIVKEILRLHPPGPL 366

Query: 241 LVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKDF 300
           LVPHESSED TI+NFYIPKKSRVIV+A AIGRDPSVWID EKFFPERFIG+Q+D+KGK F
Sbjct: 367 LVPHESSEDCTIDNFYIPKKSRVIVNAWAIGRDPSVWIDVEKFFPERFIGNQVDMKGKHF 426

Query: 301 QLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRAQ 329
           QLIPFGAGRR CPGME+GLT+VHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLT  RAQ
Sbjct: 427 QLIPFGAGRRGCPGMEMGLTIVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTSPRAQ 486

BLAST of MC01g_new0386 vs. NCBI nr
Match: XP_022132483.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 437 bits (1125), Expect = 1.39e-148
Identity = 231/369 (62.60%), Postives = 271/369 (73.44%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRK CTLELL+SHK+ SF+S+R EE+GLLIEDLREAAKSGV VD TSKIF+L L+MTCL+
Sbjct: 125 MRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEMTCLV 184

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           IFGRKY GEEV   R FKW++REA RLGAAPNL D IPCIA  DLQGLNRRAK V KV+D
Sbjct: 185 IFGRKYEGEEVH-GRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNKVLD 244

Query: 121 GFLESIIDEHLNEANSDTKDFVDVMLDIVGSRETEYQIDRSNIKAII------------- 180
           GFLESIIDEHL E+ + TKDFVD MLDI+GSR TEYQI+RS+IKA+I             
Sbjct: 245 GFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDTSAT 304

Query: 181 -----LRQLTPRPPLSN---------------------GHC----LSLSNTPMLHPPGPL 240
                L +L   P +                       GH     + +  T  LHPPGP+
Sbjct: 305 TMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPPGPI 364

Query: 241 LVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKDF 300
           L PHES ED TINNF+IPK+SR+IV+A AI RDPSVW +AEKFFPERFI S++D+KG+DF
Sbjct: 365 LAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKGRDF 424

Query: 301 QLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRAQ 326
           QLIPFGAGRRSCPG+ + L VVH++IAQLVHCFDWKLP  +LP ELD++E+ GLT  R Q
Sbjct: 425 QLIPFGAGRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCPRVQ 484

BLAST of MC01g_new0386 vs. NCBI nr
Match: XP_022133318.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 419 bits (1078), Expect = 1.41e-141
Identity = 222/373 (59.52%), Postives = 264/373 (70.78%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRKMCTLELL+ HKV SF+SMR EE+GLL+  LR+AAK  V ++ +SKI +LN DMTCLM
Sbjct: 124 MRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDMTCLM 183

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           +FG+KY   E D ERGFK V++EA++L A PNLGD IPCIA+ DLQGLNRR+K++ K+ D
Sbjct: 184 VFGKKYADAEFD-ERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAISKIFD 243

Query: 121 GFLESIIDEHLNEANSD-TKDFVDVMLDIVGSRETEYQIDRSNIKAIILRQLTP------ 180
           GF E IIDEHL   N + TKDFVDVMLDI+GS+ETEYQIDRS+IKAIIL  L        
Sbjct: 244 GFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAMDTSA 303

Query: 181 -----------------------------------RPPLSNGHCLSLSNTPML--HPPGP 240
                                                 L +   L +    +L  HP  P
Sbjct: 304 TTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVHPAAP 363

Query: 241 LLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKD 300
           LLVPHES ED T+N ++IPKKSR+IV+A AIG+DP+ W+D EKFFPERFIGSQ+DVKG+D
Sbjct: 364 LLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDVKGRD 423

Query: 301 FQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRA 329
           FQLIPFG+GRR CPGM +GL VVH VIAQLVHCFDWKLPN MLP ELDM+E+ GLT  RA
Sbjct: 424 FQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFGLTCPRA 483

BLAST of MC01g_new0386 vs. NCBI nr
Match: XP_008440675.1 (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >KAA0036257.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa] >TYK12651.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 415 bits (1067), Expect = 6.33e-140
Identity = 219/373 (58.71%), Postives = 269/373 (72.12%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRKMCTLELL+S KV+SF+SMRMEELGLL++ LR+AAK  V V+ +SKI +LN DMTCLM
Sbjct: 123 MRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDMTCLM 182

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           +FG+KY  +E D ERGFK V++EA++L A+PNLGD IP IA  DLQGL+RR+K++ K+ D
Sbjct: 183 VFGKKYEDQEFD-ERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISKIFD 242

Query: 121 GFLESIIDEHLNEANSD-TKDFVDVMLDIVGSRETEYQIDRSNIKAIILRQLTPRPPLS- 180
           GFLE IIDEHL   N + T DFVDVML+I+GS+ETEYQI+RSNIKA+IL  L      S 
Sbjct: 243 GFLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMDTSA 302

Query: 181 ------------NGHCLSLSNTPM------------------------------LHPPGP 240
                       + H +      +                              LHPP P
Sbjct: 303 TTIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHPPAP 362

Query: 241 LLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKD 300
           LLVPHE+ ED  ++ FYIPKKSR+IV+  AIGRDP+ WID EKFFPERFIGSQ+DV+G+D
Sbjct: 363 LLVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGRD 422

Query: 301 FQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRA 329
           FQLIPFG+GRR CPGM++GLTVV +VIAQLVHCFDW+LPN  LP++LDM+E+ GLT  RA
Sbjct: 423 FQLIPFGSGRRGCPGMQMGLTVVRLVIAQLVHCFDWELPNGTLPVKLDMTEEFGLTCPRA 482

BLAST of MC01g_new0386 vs. NCBI nr
Match: XP_022132564.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 405 bits (1042), Expect = 3.98e-136
Identity = 215/370 (58.11%), Postives = 270/370 (72.97%), Query Frame = 0

Query: 2   RKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLMI 61
           RKMCT+ELL++ KV SF+SMR EE+GLLI+ LREAAKS V V+ +SK+ +L  DMTCLM+
Sbjct: 126 RKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVADMTCLMV 185

Query: 62  FGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVIDG 121
           FG+KY  E++D ERGFK V++E ++L AAPNLGD IP +A  D QG NRRAKS+ KVIDG
Sbjct: 186 FGKKYGDEQLD-ERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSISKVIDG 245

Query: 122 FLESIIDEHLN-EANSDTKDFVDVMLDIVGSRETEYQIDRSNIKAIILRQLTP------- 181
           FLE IIDEHL  +  + TKDFVDVMLD++ S+ TEYQI+RS+IKAIIL  L         
Sbjct: 246 FLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQATEYQIERSSIKAIILDLLAAAVDTSAT 305

Query: 182 ----------RPPLS-------------------NGHCLSLSNTPM-------LHPPGPL 241
                     + PLS                   +    +L    M       +HPP PL
Sbjct: 306 TISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMHPPAPL 365

Query: 242 LVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKDF 301
           LVPHES +D T++NFYIPKKSR+IV+A AIGRDP+VWIDAEKFFPERFIGSQ+D+KG+DF
Sbjct: 366 LVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDIKGRDF 425

Query: 302 QLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRAQ 327
           QLIPFG+GRR CPGM++GL VV +V+AQLVHCF+W+LPN  LP +L+M+E+ GLT  RA+
Sbjct: 426 QLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLTCPRAE 485

BLAST of MC01g_new0386 vs. ExPASy TrEMBL
Match: A0A6J1BSN9 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005413 PE=3 SV=1)

HSP 1 Score: 526 bits (1356), Expect = 5.06e-184
Identity = 278/372 (74.73%), Postives = 298/372 (80.11%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRKMCTLELLNSHKVQSFKSMR EE+GLL EDLREAAK+GV VD TSKIF+LNLDMTCLM
Sbjct: 127 MRKMCTLELLNSHKVQSFKSMRREEVGLLTEDLREAAKTGVAVDLTSKIFSLNLDMTCLM 186

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           IFGRKYVG+EVD ERGFKWVV+EALRLGAAPNLGDLIPCIA FDLQGLNRRAKSVGKV+D
Sbjct: 187 IFGRKYVGKEVDDERGFKWVVQEALRLGAAPNLGDLIPCIAPFDLQGLNRRAKSVGKVLD 246

Query: 121 GFLESIIDEHLNEANSDTKDFVDVMLDIVGSRETEYQIDRSNIKAIIL------------ 180
           GFLESIIDEHL EA+++TKDFVDVMLDIVGSRETEYQIDRSNIKAIIL            
Sbjct: 247 GFLESIIDEHLREADNETKDFVDVMLDIVGSRETEYQIDRSNIKAIILDMVLATTDTTAT 306

Query: 181 ------RQLTPRPPLSNG------HCLSLSNTP-------------------MLHPPGPL 240
                  +L   P +           + LS                       LHPPGPL
Sbjct: 307 TIEWALSELVKHPRVMKKLQEELEKVVGLSGMVEESDLDYLKYLDMIVKEILRLHPPGPL 366

Query: 241 LVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKDF 300
           LVPHESSED TI+NFYIPKKSRVIV+A AIGRDPSVWID EKFFPERFIG+Q+D+KGK F
Sbjct: 367 LVPHESSEDCTIDNFYIPKKSRVIVNAWAIGRDPSVWIDVEKFFPERFIGNQVDMKGKHF 426

Query: 301 QLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRAQ 329
           QLIPFGAGRR CPGME+GLT+VHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLT  RAQ
Sbjct: 427 QLIPFGAGRRGCPGMEMGLTIVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTSPRAQ 486

BLAST of MC01g_new0386 vs. ExPASy TrEMBL
Match: A0A6J1BWD4 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005329 PE=3 SV=1)

HSP 1 Score: 437 bits (1125), Expect = 6.74e-149
Identity = 231/369 (62.60%), Postives = 271/369 (73.44%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRK CTLELL+SHK+ SF+S+R EE+GLLIEDLREAAKSGV VD TSKIF+L L+MTCL+
Sbjct: 125 MRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEMTCLV 184

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           IFGRKY GEEV   R FKW++REA RLGAAPNL D IPCIA  DLQGLNRRAK V KV+D
Sbjct: 185 IFGRKYEGEEVH-GRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNKVLD 244

Query: 121 GFLESIIDEHLNEANSDTKDFVDVMLDIVGSRETEYQIDRSNIKAII------------- 180
           GFLESIIDEHL E+ + TKDFVD MLDI+GSR TEYQI+RS+IKA+I             
Sbjct: 245 GFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDTSAT 304

Query: 181 -----LRQLTPRPPLSN---------------------GHC----LSLSNTPMLHPPGPL 240
                L +L   P +                       GH     + +  T  LHPPGP+
Sbjct: 305 TMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPPGPI 364

Query: 241 LVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKDF 300
           L PHES ED TINNF+IPK+SR+IV+A AI RDPSVW +AEKFFPERFI S++D+KG+DF
Sbjct: 365 LAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKGRDF 424

Query: 301 QLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRAQ 326
           QLIPFGAGRRSCPG+ + L VVH++IAQLVHCFDWKLP  +LP ELD++E+ GLT  R Q
Sbjct: 425 QLIPFGAGRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCPRVQ 484

BLAST of MC01g_new0386 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 419 bits (1078), Expect = 6.84e-142
Identity = 222/373 (59.52%), Postives = 264/373 (70.78%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRKMCTLELL+ HKV SF+SMR EE+GLL+  LR+AAK  V ++ +SKI +LN DMTCLM
Sbjct: 124 MRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDMTCLM 183

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           +FG+KY   E D ERGFK V++EA++L A PNLGD IPCIA+ DLQGLNRR+K++ K+ D
Sbjct: 184 VFGKKYADAEFD-ERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAISKIFD 243

Query: 121 GFLESIIDEHLNEANSD-TKDFVDVMLDIVGSRETEYQIDRSNIKAIILRQLTP------ 180
           GF E IIDEHL   N + TKDFVDVMLDI+GS+ETEYQIDRS+IKAIIL  L        
Sbjct: 244 GFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAMDTSA 303

Query: 181 -----------------------------------RPPLSNGHCLSLSNTPML--HPPGP 240
                                                 L +   L +    +L  HP  P
Sbjct: 304 TTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVHPAAP 363

Query: 241 LLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKD 300
           LLVPHES ED T+N ++IPKKSR+IV+A AIG+DP+ W+D EKFFPERFIGSQ+DVKG+D
Sbjct: 364 LLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDVKGRD 423

Query: 301 FQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRA 329
           FQLIPFG+GRR CPGM +GL VVH VIAQLVHCFDWKLPN MLP ELDM+E+ GLT  RA
Sbjct: 424 FQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFGLTCPRA 483

BLAST of MC01g_new0386 vs. ExPASy TrEMBL
Match: A0A5A7T3U2 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002390 PE=3 SV=1)

HSP 1 Score: 415 bits (1067), Expect = 3.06e-140
Identity = 219/373 (58.71%), Postives = 269/373 (72.12%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRKMCTLELL+S KV+SF+SMRMEELGLL++ LR+AAK  V V+ +SKI +LN DMTCLM
Sbjct: 123 MRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDMTCLM 182

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           +FG+KY  +E D ERGFK V++EA++L A+PNLGD IP IA  DLQGL+RR+K++ K+ D
Sbjct: 183 VFGKKYEDQEFD-ERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISKIFD 242

Query: 121 GFLESIIDEHLNEANSD-TKDFVDVMLDIVGSRETEYQIDRSNIKAIILRQLTPRPPLS- 180
           GFLE IIDEHL   N + T DFVDVML+I+GS+ETEYQI+RSNIKA+IL  L      S 
Sbjct: 243 GFLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMDTSA 302

Query: 181 ------------NGHCLSLSNTPM------------------------------LHPPGP 240
                       + H +      +                              LHPP P
Sbjct: 303 TTIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHPPAP 362

Query: 241 LLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKD 300
           LLVPHE+ ED  ++ FYIPKKSR+IV+  AIGRDP+ WID EKFFPERFIGSQ+DV+G+D
Sbjct: 363 LLVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGRD 422

Query: 301 FQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRA 329
           FQLIPFG+GRR CPGM++GLTVV +VIAQLVHCFDW+LPN  LP++LDM+E+ GLT  RA
Sbjct: 423 FQLIPFGSGRRGCPGMQMGLTVVRLVIAQLVHCFDWELPNGTLPVKLDMTEEFGLTCPRA 482

BLAST of MC01g_new0386 vs. ExPASy TrEMBL
Match: A0A1S3B1P7 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485015 PE=3 SV=1)

HSP 1 Score: 415 bits (1067), Expect = 3.06e-140
Identity = 219/373 (58.71%), Postives = 269/373 (72.12%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRKMCTLELL+S KV+SF+SMRMEELGLL++ LR+AAK  V V+ +SKI +LN DMTCLM
Sbjct: 123 MRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDMTCLM 182

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGKVID 120
           +FG+KY  +E D ERGFK V++EA++L A+PNLGD IP IA  DLQGL+RR+K++ K+ D
Sbjct: 183 VFGKKYEDQEFD-ERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISKIFD 242

Query: 121 GFLESIIDEHLNEANSD-TKDFVDVMLDIVGSRETEYQIDRSNIKAIILRQLTPRPPLS- 180
           GFLE IIDEHL   N + T DFVDVML+I+GS+ETEYQI+RSNIKA+IL  L      S 
Sbjct: 243 GFLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMDTSA 302

Query: 181 ------------NGHCLSLSNTPM------------------------------LHPPGP 240
                       + H +      +                              LHPP P
Sbjct: 303 TTIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHPPAP 362

Query: 241 LLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVKGKD 300
           LLVPHE+ ED  ++ FYIPKKSR+IV+  AIGRDP+ WID EKFFPERFIGSQ+DV+G+D
Sbjct: 363 LLVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGRD 422

Query: 301 FQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHRA 329
           FQLIPFG+GRR CPGM++GLTVV +VIAQLVHCFDW+LPN  LP++LDM+E+ GLT  RA
Sbjct: 423 FQLIPFGSGRRGCPGMQMGLTVVRLVIAQLVHCFDWELPNGTLPVKLDMTEEFGLTCPRA 482

BLAST of MC01g_new0386 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 205.3 bits (521), Expect = 7.5e-53
Identity = 119/372 (31.99%), Postives = 204/372 (54.84%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           M+ +C L LL +  V+SF+++R EE+ L++E ++++  S + V+ +  + +L  D+   +
Sbjct: 127 MKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKS--SSLQVNLSELLGSLTNDVISRV 186

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFD-LQGLNRRAKSVGKVI 120
             GRKY  +E D    FK +++   +L     +G  +P +A  D + GL+ + K  G  +
Sbjct: 187 ALGRKY-SDETD----FKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDL 246

Query: 121 DGFLESIIDEHLNEANSDTKDFVDVMLDIVGSRETEYQIDRSNIKAIIL----------- 180
           D FLE ++ +H  + ++   DFVDV+L I   +   ++IDR +IKAIIL           
Sbjct: 247 DEFLEKVVQDH-EDGDAQRTDFVDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSY 306

Query: 181 -------RQLTPRPPLSN-----------GHC--------------LSLSNTPMLHPPGP 240
                   +L  RP   N           G+                 +  T  LHPP P
Sbjct: 307 ALMEWAMTELLHRPECLNRLQEEVRTICKGNSSVSEDDIKDMNYLKAVIKETMRLHPPLP 366

Query: 241 LLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVW-IDAEKFFPERFIGSQLDVKGK 300
           L+VPHES++D  + +++IP  ++V+++A AIGR+ + W  DAEKF PER + S +D +G 
Sbjct: 367 LMVPHESTQDVRLGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLNSSVDFRGH 426

Query: 301 DFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTFHR 328
           +F+LIPFGAGRR CP +   + ++ + +A LVH +DW+LP + +  + +++E +G+  H 
Sbjct: 427 NFELIPFGAGRRICPAISFAVILIEVTLANLVHRYDWRLPEEYIEDQTNVAESTGMVIH- 485

BLAST of MC01g_new0386 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 205.3 bits (521), Expect = 7.5e-53
Identity = 132/394 (33.50%), Postives = 201/394 (51.02%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           MRK+C +++ +  + +S+ S+R +E+  ++  +  +   G P++   +IFAL  ++T   
Sbjct: 135 MRKVCVMKVFSRKRAESWASVR-DEVDKMVRSV--SCNVGKPINVGEQIFALTRNITYRA 194

Query: 61  IFGRKYVGEEVDVERG---FKWVVREALRLGAAPNLGDLIPCIASFDLQGLNRRAKSVGK 120
            FG          E+G   F  +++E  +L  A N+ D IP     D QG+N+R      
Sbjct: 195 AFGSA-------CEKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARN 254

Query: 121 VIDGFLESIIDEHL----NEANSDTKDFVDV---------------MLDIVGSRETEYQI 180
            +DGF++ IIDEH+    N+   D  D VD                ++      +   ++
Sbjct: 255 DLDGFIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKL 314

Query: 181 DRSNIKAII------------------LRQLTPRP------------------------- 240
            R NIKAII                  L +L   P                         
Sbjct: 315 TRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDI 374

Query: 241 -PLSNGHCLSLSNTPMLHPPGPLLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVW 300
             L+   C +L  T  +HPP PLL+ HE++ED +I+ F+IPKKSRV+++A AIGRDP+ W
Sbjct: 375 EKLTYLKC-TLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSW 434

Query: 301 IDAEKFFPERFIGSQL-DVKGKDFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWK 328
            D + F P RF+   + D KG +F+ IPFG+GRRSCPGM+LGL  + + +A ++HCF WK
Sbjct: 435 TDPDTFRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWK 494

BLAST of MC01g_new0386 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 199.9 bits (507), Expect = 3.2e-51
Identity = 117/370 (31.62%), Postives = 184/370 (49.73%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           +RK+   EL +S +V S + ++ EE+  LI+ + E+A    P++    + AL + + C  
Sbjct: 126 VRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRT 185

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASFD--LQGLNRRAKSVGKV 120
            F   + G  ++ ER F  +VREAL +  + +  D IP +      L GL  R +   + 
Sbjct: 186 AFSVNFEGTVLNSER-FNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRD 245

Query: 121 IDGFLESIIDEHLNEANSDTKDFVDVMLDIVGSRET--EYQIDRSNIKAIILRQLTP--- 180
           +D F E + D H  +    ++DFVD++L +          ++ R++IKAI++  L     
Sbjct: 246 LDAFYEQMFDLHKQKKEEGSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAGMD 305

Query: 181 ---------------------------RPPLSNGHCLSLSNTP-------------MLHP 240
                                      R  + N   +S  +T               LHP
Sbjct: 306 TSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHP 365

Query: 241 PGPLLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQLDVK 300
             PLL+P E+  +F IN + IP K+R+ V+  AIGRDP  W D E F PERF  + +D K
Sbjct: 366 TTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNIDAK 425

Query: 301 GKDFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGLTF 324
           G+ F+L+PFG GRR CP + +G T+V   +A L++ FDWKLP  M   ++DM E  GLT 
Sbjct: 426 GQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGLTV 485

BLAST of MC01g_new0386 vs. TAIR 10
Match: AT3G26290.1 (cytochrome P450, family 71, subfamily B, polypeptide 26 )

HSP 1 Score: 199.1 bits (505), Expect = 5.4e-51
Identity = 117/375 (31.20%), Postives = 189/375 (50.40%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           +RK+C+ EL +++K+QS + ++ EE+  +I+ + E++    PV+ +    AL   + C  
Sbjct: 126 LRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSVVCKA 185

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGAAPNLGDLIPCIASF--DLQGLNRRAKSVGKV 120
            FG  + G  ++ +R F  +VR+   +  + +  D IP +        GL    K   + 
Sbjct: 186 AFGVSFEGSVLNSDR-FNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGLQGWRKKSFRD 245

Query: 121 IDGFLESIIDEHLNEANSDTKDFVDVMLD------IVGSRETEYQIDRSNIKAIILR--- 180
           +D F E I D H  E    ++D VDV+L       +VG+     ++ R++IKAI++    
Sbjct: 246 LDAFYEQIFDLHKEEKEVGSEDLVDVLLRLEKEEIVVGNG----KLTRNHIKAILMNILL 305

Query: 181 ---------------------------QLTPRPPLSNGHCLSLSNTP------------- 240
                                      Q   R  + N   +S  +T              
Sbjct: 306 GGIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETW 365

Query: 241 MLHPPGPLLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGSQ 300
            LHPP PLL+P +   +F IN + IP K+R+ V+  AIGRDP  W D E F PERF  S 
Sbjct: 366 RLHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSN 425

Query: 301 LDVKGKDFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEKS 325
           +D KG++F+L+ FG+GRR CPG+ +G T+V   +A +++ FDWKLP  M+  ++DM E  
Sbjct: 426 IDAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAP 485

BLAST of MC01g_new0386 vs. TAIR 10
Match: AT3G26320.1 (cytochrome P450, family 71, subfamily B, polypeptide 36 )

HSP 1 Score: 198.4 bits (503), Expect = 9.2e-51
Identity = 117/376 (31.12%), Postives = 192/376 (51.06%), Query Frame = 0

Query: 1   MRKMCTLELLNSHKVQSFKSMRMEELGLLIEDLREAAKSGVPVDPTSKIFALNLDMTCLM 60
           +R++C  EL +  +VQSF+ ++ +E+  LI+ + E+A  G PV+ + K  +L + +TC  
Sbjct: 126 LRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKA 185

Query: 61  IFGRKYVGEEVDVERGFKWVVREALRLGA------APNLGDLIPCIASFDLQGLNRRAKS 120
            FG  + G  ++ +R  K +    L LG+       PN G +I       L GL+ + + 
Sbjct: 186 TFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDW-----LTGLHGQRER 245

Query: 121 VGKVIDGFLESIIDEHLNEANSDTKDFVDVMLDIVGSRETEY---QIDRSNIKAIILRQL 180
             + +D F E + D H        +DFVD++L +    ET     ++ R++IKAI++  L
Sbjct: 246 SVRALDAFYEQMFDLHKQGNKEGVEDFVDLLLRL-EKEETVIGYGKLTRNHIKAILMNVL 305

Query: 181 --------------------TPR---------------------PPLSNGHCLS--LSNT 240
                                PR                       +   H L   ++ T
Sbjct: 306 IGGIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINET 365

Query: 241 PMLHPPGPLLVPHESSEDFTINNFYIPKKSRVIVSACAIGRDPSVWIDAEKFFPERFIGS 300
             LHPP P L+P +   +F +N++ IP K+R+ V+  AIGRDP  W D E+F PERF+ S
Sbjct: 366 WRLHPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNS 425

Query: 301 QLDVKGKDFQLIPFGAGRRSCPGMELGLTVVHMVIAQLVHCFDWKLPNDMLPIELDMSEK 325
            +D KG+ F+L+PFG+GRR CP M +G T+V   +A +++ FDWK+P  M+  ++D+ E 
Sbjct: 426 SIDAKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEES 485