MC01g_new0374 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0374 vs. ExPASy Swiss-Prot
Match: Q8VYW6 (Glutathione hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=GGT1 PE=2 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.5e-205
Identity = 376/573 (65.62%), Postives = 443/573 (77.31%), Query Frame = 0

Query: 2   LMRWLIIVLVSTALLPPTFI-ARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 61
           L+R + IVL   A L       R +  V S GAVA DDGRCS IGM VLREGG+A+DASV
Sbjct: 3   LVRTVTIVLFIIAFLQNAAAQKRQQSIVKSRGAVATDDGRCSVIGMRVLREGGNAIDASV 62

Query: 62  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 121
           AAALCLGVVSPASSGIGGGAF +++ A G   A D RETAP  A+ENMY GN   K  GA
Sbjct: 63  AAALCLGVVSPASSGIGGGAFTVVKIAGGKEIAYDSRETAPLRATENMYGGNVDLKKKGA 122

Query: 122 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 181
           LS+GVPGE+AGL  AWKQHG+L W RLV PAE+LA  GFKIS YL+MQM  T   IL D+
Sbjct: 123 LSVGVPGEVAGLFTAWKQHGKLPWKRLVTPAEKLAE-GFKISKYLYMQMNATRSDILADK 182

Query: 182 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 241
           GL +LF S G+L KPG +  N KLA TL+ I  +G KAFYNG++G+NL RDI+K GGI++
Sbjct: 183 GLSDLFVSNGELKKPGTICHNPKLALTLKLIGEYGPKAFYNGTVGVNLARDIKKSGGIIT 242

Query: 242 MKDLQSYQVKLRVPILARTMGLELITMPPP-SGGSGVILMLNILAQYKPPSGLSGPLGIH 301
           +KDLQSY+VK++ P+ A  +G  ++ MPPP SGG+ ++L+LNIL+QY  PSG+SGPLG+H
Sbjct: 243 LKDLQSYRVKIKEPLSADILGYRVLGMPPPSSGGAAMMLVLNILSQYGIPSGVSGPLGVH 302

Query: 302 REIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWN 361
           R IEALKH  AVR NLGDP F +V++V+S+MLSPKFA+ LK  I D +TFDP +YGG WN
Sbjct: 303 RLIEALKHAFAVRMNLGDPDFTDVTKVVSDMLSPKFAKDLKSKINDQKTFDPKYYGGMWN 362

Query: 362 PINDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVP--ANVSA 421
            I+DHGTSHLSI+D ERNAVSMT+T+N YFGA +LSPSTGIVLNNEMDDFS+P  +N + 
Sbjct: 363 QIDDHGTSHLSIIDRERNAVSMTSTINGYFGALMLSPSTGIVLNNEMDDFSIPMKSNGNL 422

Query: 422 NKLPPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGM 481
           +  PPAPANFIRPGKRPLSSMSPTIVLKDGK+K  VGASGG  IIAGT EV LNHF   M
Sbjct: 423 DVPPPAPANFIRPGKRPLSSMSPTIVLKDGKVKAAVGASGGANIIAGTTEVYLNHFFLKM 482

Query: 482 DPLSSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQ 541
           DPLSSV+APR+YH LIPN  +YENWTTV  DHFE+P  TR VL KKGHVL  +AGGTI Q
Sbjct: 483 DPLSSVLAPRIYHQLIPNRASYENWTTVFNDHFEIPKATRVVLEKKGHVLSPIAGGTIAQ 542

Query: 542 FIVQDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 571
           FIVQ+ S    G G  +LVAVSDPRKGG PSG+
Sbjct: 543 FIVQE-SGENSG-GRSELVAVSDPRKGGFPSGY 572

BLAST of MC01g_new0374 vs. ExPASy Swiss-Prot
Match: Q680I5 (Glutathione hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=GGT2 PE=2 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 6.9e-200
Identity = 361/574 (62.89%), Postives = 434/574 (75.61%), Query Frame = 0

Query: 2   LMRWLIIVLVSTALLP-PTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 61
           L+R   I L+  A L     +   +  VA HGAVA DDGRCS IG  VLR+GG+A+DASV
Sbjct: 7   LVRTATIALLLIAFLQNANAVKNLQSIVAYHGAVATDDGRCSAIGTNVLRQGGNAIDASV 66

Query: 62  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 121
           AAALCLGVVSPASSGIGGGAF +I+ A+G   A D RETAP  A+E+MY  N   K  G+
Sbjct: 67  AAALCLGVVSPASSGIGGGAFTMIKLANGTEVAYDSRETAPLSATEDMYGDNPERKKKGS 126

Query: 122 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 181
           LS+GVPGE+AGL+ AW QHG+L W +LV+PAE+LA  GFKIS YL+MQM  T   IL D+
Sbjct: 127 LSVGVPGEVAGLYTAWTQHGKLPWKQLVEPAEKLAAEGFKISKYLYMQMNATRSDILADK 186

Query: 182 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 241
           GL  LF S G+L KPG + RN KLA TL  I+ +G KAFYNG++G NL+ DI+K GGI++
Sbjct: 187 GLSELFVSNGELKKPGAICRNPKLADTLSQIAEYGPKAFYNGTVGFNLVSDIQKAGGIIT 246

Query: 242 MKDLQSYQVKLRVPILARTMGLELITMPPP-SGGSGVILMLNILAQYKPPSGLSGPLGIH 301
           +KDLQ+Y VK++ P+    +G  L+ MPPP SGG  ++L+LNILAQY  PSG+SGPLG+H
Sbjct: 247 LKDLQNYNVKVKEPLSTEILGYRLLGMPPPSSGGPAMMLILNILAQYGIPSGVSGPLGVH 306

Query: 302 REIEALKHVIAVRTNLGDPSFF-NVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRW 361
           R +EALKH  AVR NLGDP F   V+ V+++MLSPKFAQ LK  I D +TFDP +YGG+W
Sbjct: 307 RLVEALKHAFAVRMNLGDPDFVPEVTNVVADMLSPKFAQDLKSKINDEKTFDPKYYGGKW 366

Query: 362 NPINDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSAN 421
             I DHGTSHLSI+D+ERNAVSMT+T+N YFGA +LSPSTGIVLNNEMDDFS+P     +
Sbjct: 367 GQIKDHGTSHLSIIDSERNAVSMTSTINGYFGAIMLSPSTGIVLNNEMDDFSIPTKSGGD 426

Query: 422 K--LPPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKG 481
               PPAPANFIRPGKRPLSSM+PTIVLKDGK+K  +GASGG +IIAGT +V LNHF   
Sbjct: 427 PDVPPPAPANFIRPGKRPLSSMTPTIVLKDGKVKAALGASGGMYIIAGTTQVYLNHFFLN 486

Query: 482 MDPLSSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTIC 541
           MDPLSSV+APR+YH LIPN  +YENWTTV  DHFE+P + R VL KKG VL  +AGGTI 
Sbjct: 487 MDPLSSVVAPRIYHQLIPNKASYENWTTVYSDHFEIPEEIRLVLEKKGQVLTPIAGGTIS 546

Query: 542 QFIVQDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 571
           Q IV+   S GK  G+ KLVAVSDPRKGG PSG+
Sbjct: 547 QLIVE--QSDGKSGGISKLVAVSDPRKGGFPSGY 578

BLAST of MC01g_new0374 vs. ExPASy Swiss-Prot
Match: Q9M0G0 (Glutathione hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=GGT3 PE=2 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 6.8e-155
Identity = 284/562 (50.53%), Postives = 385/562 (68.51%), Query Frame = 0

Query: 25  EVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASVAAALCLGVVSPASSGIGGGAFMLI 84
           +V  + +G VAADD RCS IG  VLR GGHAVDA+VA  LC+GVV+P SSGIGGG+F+++
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRSGGHAVDAAVAITLCVGVVNPMSSGIGGGSFLIV 133

Query: 85  RS-ADGHSQALDMRETAPFLASENMYAGNAASKAAGALSIGVPGELAGLHEAWKQHGRLS 144
            S  D  ++A DMRETAP  AS++MY  +A++K+ GALS+GVPGE+AGL+EAWK++GRL 
Sbjct: 134 SSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRLP 193

Query: 145 WARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDEGLRNLFTSGGKLLKPGDVVRNKK 204
           W  L +PA +LAR GF + PYL   +      IL D G+R++F+  G++LK G+   N +
Sbjct: 194 WKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSRNGQVLKTGETCYNPE 253

Query: 205 LAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILSMKDLQSYQVKLRVPILARTMGLE 264
           LA++L TIS  G  AFYNG++G  L++D++K GGI++M DL+SY+V++   +    MG  
Sbjct: 254 LAQSLETISEQGPGAFYNGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAMSVDVMGYT 313

Query: 265 LITMPPPSGGS-GVILMLNILAQYKPPSGLSG-PLGIHREIEALKHVIAVRTNLGDPSFF 324
           +  MPPPSGG+ G  +++NIL  Y      SG  LG+HR IEA+KH+ A R +LGDP F 
Sbjct: 314 VHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMDLGDPEFV 373

Query: 325 NVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNPINDHGTSHLSIVDAERNAVSM 384
           NV+  ++ MLS   A++++K I+DN TF P +Y  RW+ + D GTSH  +VDA+RN+VSM
Sbjct: 374 NVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDADRNSVSM 433

Query: 385 TTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKLPPAPANFIRPGKRPLSSMSPT 444
           T+TVN  FGA +LSPSTGIVLNNEMDDFS P  ++ + LPPAP NFI P KRPLSSM+P 
Sbjct: 434 TSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRPLSSMTPL 493

Query: 445 IVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPLSSVMAPRVYHPLIPNVVNYEN 504
           ++ KDG+    +G +GG  II    +V LN F   M P  +V + R+YH LIPNVV+YEN
Sbjct: 494 VITKDGEFVAALGGAGGMHIIPAVLQVFLNCFVLNMKPKEAVESARIYHRLIPNVVSYEN 553

Query: 505 WTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIVQDYS---------SLGKGVG- 564
           +TT+ GDH  V   T+  LA++GH L+ L+GG I Q IVQ +           +G+ +G 
Sbjct: 554 FTTINGDHIGVSEDTKMFLAERGHELKELSGGAIVQLIVQSFKEEKEEEMIIEIGRKIGK 613

Query: 565 -----VGKLVAVSDPRKGGVPS 569
                 G L AVSDPRK G P+
Sbjct: 614 KSKPLKGLLTAVSDPRKDGKPA 635

BLAST of MC01g_new0374 vs. ExPASy Swiss-Prot
Match: Q9US04 (Glutathione hydrolase proenzyme 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ggt1 PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 6.9e-99
Identity = 204/552 (36.96%), Postives = 317/552 (57.43%), Query Frame = 0

Query: 26  VTVASHGAVAADDGRCSRIGMEVLREGGHAVDASVAAALCLGVVSPASSGIGGGAFMLIR 85
           V    +G VA ++  CS+IG+ +L+ GG+AVDA++A+ +C+G V+  SSGIGGG FMLIR
Sbjct: 94  VVEGKNGVVATEEETCSQIGVGILKAGGNAVDAAIASGICIGAVNSFSSGIGGGGFMLIR 153

Query: 86  SADGHSQALDMRETAPFLASENMYAGNAASKAAGALSIGVPGELAGLHEAWKQHGRLSWA 145
             +G + +L+ RETAP  AS+NM+ GN+     G LS+ VPGE+AG   AWK +G L W 
Sbjct: 154 HPNGTAHSLNFRETAPAGASKNMFHGNSTLSQVGGLSVAVPGEIAGYERAWKMYGSLPWH 213

Query: 146 RLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE-GLRNLFTSGGKLLKPGDVVRNKKL 205
           +L +P  RL R G  +   L  ++ R E            +F   G  L  G+      L
Sbjct: 214 KLFEPTIRLMRDGMPMPKELASRIRRPEFSYFKTHPDWSKIFAPEGVFLHVGEKFYRPAL 273

Query: 206 AKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILSMKDLQSYQVKLRVPILARTMGLEL 265
           A TL  I++FG + FY G I   L++ +++ GGIL+M+D+ ++ V +  PI       E+
Sbjct: 274 ASTLEEIAKFGPEVFYTGKIAERLVKFVQQQGGILTMEDMANFSVVVEEPIYGNFYDREV 333

Query: 266 ITMPPPSGGSGVILMLNILAQYKPPSGLS------GPLGIHREIEALKHVIAVRTNLGDP 325
           IT   P  G  +IL LN+L++     G S        +G+H  IE +K + A RT L DP
Sbjct: 334 ITCGSPCSGEALILGLNVLSKVDLSEGTSILGCEMTDIGVHHLIETMKWMSAGRTVLADP 393

Query: 326 SFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNPINDHGTSHLSIVDAERNA 385
           +F+N ++ +  +LS ++A +++  I + RTFD +HY   ++  NDHGT+HLS++D +  A
Sbjct: 394 TFYNNTDHVEQLLSLEYADEIRNNISNERTFDFTHYKAEYDFPNDHGTTHLSVIDKDNMA 453

Query: 386 VSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKLPPAPANFIRPGKRPLSSM 445
           V +T ++N  FG+ +L P TGI+LN+ MDDF+ P  V+A  L P+P NFI PGKRP SS 
Sbjct: 454 VGLTASINLMFGSQLLEPETGIILNDHMDDFASPGIVNAFGLSPSPYNFIAPGKRPQSSA 513

Query: 446 SPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPLSSVMAPRVYHPLIPNVVN 505
            PTI++ +G+++ V+G SGG  I+    + ++  +  G   L SV +PR +H L+PN+V 
Sbjct: 514 VPTILVYNGEVEMVLGGSGGSRIVTAVLDTIIKKYKWGKSLLESVESPRFHHQLMPNIVY 573

Query: 506 YENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIVQDYSSLGKGVGVGKLVAV 565
            +           V  +    L K GH+++ +      Q+   +  ++ +  G   L  +
Sbjct: 574 IDE---------TVEIEVLRALEKFGHIVDLIP----VQYPFSEIQAVFRTNGT--LYGL 630

Query: 566 SDPRKGGVPSGF 571
           SD RK  V + +
Sbjct: 634 SDSRKQAVAAAY 630

BLAST of MC01g_new0374 vs. ExPASy Swiss-Prot
Match: Q60928 (Glutathione hydrolase 1 proenzyme OS=Mus musculus OX=10090 GN=Ggt1 PE=1 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 2.7e-95
Identity = 222/573 (38.74%), Postives = 333/573 (58.12%), Query Frame = 0

Query: 6   LIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASVAAALC 65
           L+ V++   +  P    +P+  V S  AVA D  RCS IG ++L+EGG  VDA++A+ LC
Sbjct: 15  LVFVIIGLCIWLPYTSGKPD-HVYSRAAVATDAKRCSEIGRDILQEGGSVVDAAIASLLC 74

Query: 66  LGVVSPASSGIGGGAFMLI-RSADGHSQALDMRETAPFLASENMYAGNAASKAAGALSIG 125
           +G+++  S GIGGG F  I  S  G  + ++ RE AP LA+  M+  N+     G LS+ 
Sbjct: 75  MGLMNAHSMGIGGGLFFTIYNSTTGKVEVINAREVAPRLANTTMF-NNSKDSEEGGLSVA 134

Query: 126 VPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDEGLRN 185
           VPGE+ G   A ++HGRL WARL QP+ +LAR GF +   L + + +    I     L  
Sbjct: 135 VPGEIRGYELAHQRHGRLPWARLFQPSIQLARHGFPVGKGLAIALDKKRDVIEKTPALCE 194

Query: 186 LFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILSMKDL 245
           +F   GK+L+ G+ V   KLA TL+ +++ G KAFYNGS+   +++DI++ GGI++++DL
Sbjct: 195 VFCRQGKVLQEGETVTMPKLADTLQILAQEGAKAFYNGSLTAQIVKDIQEAGGIMTVEDL 254

Query: 246 QSYQVKLRVPILARTMGLELITMP-PPSGGSGVILMLNILAQYK-PPSGLSGP----LGI 305
            +Y+ +L    ++  +G   + +P  P  G  +IL+LNIL  Y   P  ++ P    L  
Sbjct: 255 NNYRAELIEHPMSIGLGDATLYVPSAPLSGPVLILILNILKGYNFSPKSVATPEQKALTY 314

Query: 306 HREIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRW 365
           HR +EA +   A RT LGDP F +VS+V+ NM S  +A QL+  I D  T   ++Y   +
Sbjct: 315 HRIVEAFRFAYAKRTMLGDPKFVDVSQVIRNMSSEFYATQLRARITDETTHPAAYYEPEF 374

Query: 366 NPINDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSAN 425
              +D GT+HLS V  + +AV+ T+T+N YFG+ +LS  +GI+ N+EMDDFS P  ++  
Sbjct: 375 YLQDDGGTAHLSAVSEDGSAVAATSTINLYFGSKVLSRVSGILFNDEMDDFSSPNFINQF 434

Query: 426 KLPPAPANFIRPGKRPLSSMSPTIVL-KDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGM 485
           ++ P+PANFI+PGK+PLSSM P+I+L KDG+++ VVGASGG  I    A  ++N    G 
Sbjct: 435 RVAPSPANFIKPGKQPLSSMCPSIILDKDGQVRMVVGASGGTQITTSVALAIINSLWFGY 494

Query: 486 DPLSSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQ 545
           D   +V  PR+++ L+PN       TTV  D  +V   T  +  +  H         + Q
Sbjct: 495 DVKRAVEEPRLHNQLLPNT------TTVEKDIDQV--VTAGLKIRHHHTEVTPTFIAVVQ 554

Query: 546 FIVQDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 571
            +V+           G   A SD RKGG P+G+
Sbjct: 555 AVVR---------ASGGWAAASDSRKGGEPAGY 568

BLAST of MC01g_new0374 vs. NCBI nr
Match: XP_022133391.1 (gamma-glutamyltranspeptidase 1 [Momordica charantia] >XP_022133392.1 gamma-glutamyltranspeptidase 1 [Momordica charantia] >XP_022133393.1 gamma-glutamyltranspeptidase 1 [Momordica charantia] >XP_022133394.1 gamma-glutamyltranspeptidase 1 [Momordica charantia])

HSP 1 Score: 1109 bits (2868), Expect = 0.0
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV
Sbjct: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA
Sbjct: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE
Sbjct: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS
Sbjct: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR
Sbjct: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP
Sbjct: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL
Sbjct: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL
Sbjct: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV
Sbjct: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF
Sbjct: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570

BLAST of MC01g_new0374 vs. NCBI nr
Match: XP_004143492.1 (glutathione hydrolase 1 isoform X2 [Cucumis sativus] >XP_011657996.1 glutathione hydrolase 1 isoform X2 [Cucumis sativus] >XP_011657997.1 glutathione hydrolase 1 isoform X2 [Cucumis sativus] >XP_031743432.1 glutathione hydrolase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 985 bits (2547), Expect = 0.0
Identity = 502/570 (88.07%), Postives = 533/570 (93.51%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           ML+RWL+IVLVS  LL PT IARPEV VASHGAVAADD RCS+IGM+VLREGGHAVDASV
Sbjct: 1   MLIRWLVIVLVSIGLLTPTSIARPEVVVASHGAVAADDRRCSKIGMDVLREGGHAVDASV 60

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVSPASSGIGGGAFMLIRSA G +QA DMRETAP LASENMYAGN ASKA+GA
Sbjct: 61  AAALCLGVVSPASSGIGGGAFMLIRSASGKAQAFDMRETAPLLASENMYAGNVASKASGA 120

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSI VPGELAGLHEAWKQHG+L WARLVQPAERLARLGFKISPYLHMQMVRTE GILGDE
Sbjct: 121 LSIAVPGELAGLHEAWKQHGKLPWARLVQPAERLARLGFKISPYLHMQMVRTESGILGDE 180

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNL TSGGKLLK GD+VRN+KLA+TLRTISRFGV AFYNGSIGINLIRDIRKLGG+LS
Sbjct: 181 GLRNLLTSGGKLLKQGDIVRNQKLAETLRTISRFGVSAFYNGSIGINLIRDIRKLGGLLS 240

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSYQVK RVPIL+RTM LE+ TMPPPSGGSGVILMLNILAQYK PSGL GPLGIHR
Sbjct: 241 MKDLQSYQVKTRVPILSRTMDLEITTMPPPSGGSGVILMLNILAQYKLPSGLLGPLGIHR 300

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHV+AVRTNLGDP F+NV+EVLS+MLSPKFA+QLKKTI DN+TF PSHYGGRWNP
Sbjct: 301 EIEALKHVVAVRTNLGDPDFYNVTEVLSDMLSPKFAEQLKKTINDNKTFHPSHYGGRWNP 360

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           INDHGTSHLS+VD ERNAVSMTTTVN YFGAHILSPSTGIVLNNEMDDF++PANVSAN+L
Sbjct: 361 INDHGTSHLSVVDGERNAVSMTTTVNYYFGAHILSPSTGIVLNNEMDDFAIPANVSANQL 420

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAPANFIRPGKRPLSSMSPTIVLK+G+LK VVGASGGGFIIAGTAEVLLNHFAKGM P 
Sbjct: 421 PPAPANFIRPGKRPLSSMSPTIVLKNGQLKAVVGASGGGFIIAGTAEVLLNHFAKGMSPF 480

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYH L+PN++NYENWTTVLGDHFEVPA+TRAVLAKKGHVLEGLAGGTICQFIV
Sbjct: 481 SSVMAPRVYHQLVPNILNYENWTTVLGDHFEVPAETRAVLAKKGHVLEGLAGGTICQFIV 540

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           Q  SSL +G G GKLVAVSDPRKGG P+GF
Sbjct: 541 QGSSSLRQGDGFGKLVAVSDPRKGGAPAGF 570

BLAST of MC01g_new0374 vs. NCBI nr
Match: XP_031743431.1 (glutathione hydrolase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 985 bits (2547), Expect = 0.0
Identity = 502/570 (88.07%), Postives = 533/570 (93.51%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           ML+RWL+IVLVS  LL PT IARPEV VASHGAVAADD RCS+IGM+VLREGGHAVDASV
Sbjct: 20  MLIRWLVIVLVSIGLLTPTSIARPEVVVASHGAVAADDRRCSKIGMDVLREGGHAVDASV 79

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVSPASSGIGGGAFMLIRSA G +QA DMRETAP LASENMYAGN ASKA+GA
Sbjct: 80  AAALCLGVVSPASSGIGGGAFMLIRSASGKAQAFDMRETAPLLASENMYAGNVASKASGA 139

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSI VPGELAGLHEAWKQHG+L WARLVQPAERLARLGFKISPYLHMQMVRTE GILGDE
Sbjct: 140 LSIAVPGELAGLHEAWKQHGKLPWARLVQPAERLARLGFKISPYLHMQMVRTESGILGDE 199

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNL TSGGKLLK GD+VRN+KLA+TLRTISRFGV AFYNGSIGINLIRDIRKLGG+LS
Sbjct: 200 GLRNLLTSGGKLLKQGDIVRNQKLAETLRTISRFGVSAFYNGSIGINLIRDIRKLGGLLS 259

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSYQVK RVPIL+RTM LE+ TMPPPSGGSGVILMLNILAQYK PSGL GPLGIHR
Sbjct: 260 MKDLQSYQVKTRVPILSRTMDLEITTMPPPSGGSGVILMLNILAQYKLPSGLLGPLGIHR 319

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHV+AVRTNLGDP F+NV+EVLS+MLSPKFA+QLKKTI DN+TF PSHYGGRWNP
Sbjct: 320 EIEALKHVVAVRTNLGDPDFYNVTEVLSDMLSPKFAEQLKKTINDNKTFHPSHYGGRWNP 379

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           INDHGTSHLS+VD ERNAVSMTTTVN YFGAHILSPSTGIVLNNEMDDF++PANVSAN+L
Sbjct: 380 INDHGTSHLSVVDGERNAVSMTTTVNYYFGAHILSPSTGIVLNNEMDDFAIPANVSANQL 439

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAPANFIRPGKRPLSSMSPTIVLK+G+LK VVGASGGGFIIAGTAEVLLNHFAKGM P 
Sbjct: 440 PPAPANFIRPGKRPLSSMSPTIVLKNGQLKAVVGASGGGFIIAGTAEVLLNHFAKGMSPF 499

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYH L+PN++NYENWTTVLGDHFEVPA+TRAVLAKKGHVLEGLAGGTICQFIV
Sbjct: 500 SSVMAPRVYHQLVPNILNYENWTTVLGDHFEVPAETRAVLAKKGHVLEGLAGGTICQFIV 559

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           Q  SSL +G G GKLVAVSDPRKGG P+GF
Sbjct: 560 QGSSSLRQGDGFGKLVAVSDPRKGGAPAGF 589

BLAST of MC01g_new0374 vs. NCBI nr
Match: KAE8647568.1 (hypothetical protein Csa_003330 [Cucumis sativus])

HSP 1 Score: 985 bits (2547), Expect = 0.0
Identity = 502/570 (88.07%), Postives = 533/570 (93.51%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           ML+RWL+IVLVS  LL PT IARPEV VASHGAVAADD RCS+IGM+VLREGGHAVDASV
Sbjct: 29  MLIRWLVIVLVSIGLLTPTSIARPEVVVASHGAVAADDRRCSKIGMDVLREGGHAVDASV 88

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVSPASSGIGGGAFMLIRSA G +QA DMRETAP LASENMYAGN ASKA+GA
Sbjct: 89  AAALCLGVVSPASSGIGGGAFMLIRSASGKAQAFDMRETAPLLASENMYAGNVASKASGA 148

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSI VPGELAGLHEAWKQHG+L WARLVQPAERLARLGFKISPYLHMQMVRTE GILGDE
Sbjct: 149 LSIAVPGELAGLHEAWKQHGKLPWARLVQPAERLARLGFKISPYLHMQMVRTESGILGDE 208

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNL TSGGKLLK GD+VRN+KLA+TLRTISRFGV AFYNGSIGINLIRDIRKLGG+LS
Sbjct: 209 GLRNLLTSGGKLLKQGDIVRNQKLAETLRTISRFGVSAFYNGSIGINLIRDIRKLGGLLS 268

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSYQVK RVPIL+RTM LE+ TMPPPSGGSGVILMLNILAQYK PSGL GPLGIHR
Sbjct: 269 MKDLQSYQVKTRVPILSRTMDLEITTMPPPSGGSGVILMLNILAQYKLPSGLLGPLGIHR 328

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHV+AVRTNLGDP F+NV+EVLS+MLSPKFA+QLKKTI DN+TF PSHYGGRWNP
Sbjct: 329 EIEALKHVVAVRTNLGDPDFYNVTEVLSDMLSPKFAEQLKKTINDNKTFHPSHYGGRWNP 388

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           INDHGTSHLS+VD ERNAVSMTTTVN YFGAHILSPSTGIVLNNEMDDF++PANVSAN+L
Sbjct: 389 INDHGTSHLSVVDGERNAVSMTTTVNYYFGAHILSPSTGIVLNNEMDDFAIPANVSANQL 448

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAPANFIRPGKRPLSSMSPTIVLK+G+LK VVGASGGGFIIAGTAEVLLNHFAKGM P 
Sbjct: 449 PPAPANFIRPGKRPLSSMSPTIVLKNGQLKAVVGASGGGFIIAGTAEVLLNHFAKGMSPF 508

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYH L+PN++NYENWTTVLGDHFEVPA+TRAVLAKKGHVLEGLAGGTICQFIV
Sbjct: 509 SSVMAPRVYHQLVPNILNYENWTTVLGDHFEVPAETRAVLAKKGHVLEGLAGGTICQFIV 568

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           Q  SSL +G G GKLVAVSDPRKGG P+GF
Sbjct: 569 QGSSSLRQGDGFGKLVAVSDPRKGGAPAGF 598

BLAST of MC01g_new0374 vs. NCBI nr
Match: XP_023003228.1 (glutathione hydrolase 1 isoform X3 [Cucurbita maxima] >XP_023003230.1 glutathione hydrolase 1 isoform X3 [Cucurbita maxima])

HSP 1 Score: 980 bits (2534), Expect = 0.0
Identity = 502/570 (88.07%), Postives = 535/570 (93.86%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           MLMRWLIIVLVS ALL PT IARPEV VASHGAVAADD RCSRIGM+VLREGGHAVDAS+
Sbjct: 1   MLMRWLIIVLVSIALLIPTSIARPEVIVASHGAVAADDSRCSRIGMDVLREGGHAVDASI 60

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVS ASSGIGGGAFMLIRSA G SQA DMRETAP LA EN+YA N ASKAAGA
Sbjct: 61  AAALCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENIYASNVASKAAGA 120

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSI VPGELAGLHEAWKQ+G+L WARLVQPAERLARLGFKISPYLHMQMV+TE GILGDE
Sbjct: 121 LSIAVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDE 180

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNLFTSGGKLLKPGDV+RNKKLA+TLRTISRFGV+AFYNGSIGINLIRDIRKLGGILS
Sbjct: 181 GLRNLFTSGGKLLKPGDVIRNKKLAQTLRTISRFGVQAFYNGSIGINLIRDIRKLGGILS 240

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSY+VK+RVPI ARTMG E+ITMPPPSGGSG+ILMLNILAQYK PSGL GPLGIHR
Sbjct: 241 MKDLQSYRVKIRVPISARTMGHEIITMPPPSGGSGLILMLNILAQYKLPSGLLGPLGIHR 300

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHV AVRTNLGDP+F+NVS+VLS+MLSPKFA++LKKTI DNRTFDPSHYGGRWNP
Sbjct: 301 EIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGRWNP 360

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           I+DHGTSHLS+VD ERNAVSMTTTVN YFGA ILSPSTGI+LNNEMDDFSVPANVSANKL
Sbjct: 361 IHDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSANKL 420

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAP+NFIRPGKRPLSSMSP IVLK+G+LKGVVGASGGGFIIAGTAEV+LNHFAKGMDP 
Sbjct: 421 PPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGMDPF 480

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYH LIPNV+ YENWTTVLGDHFEVPA+TRA LAKKGHVLEGLAGGTICQFIV
Sbjct: 481 SSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAGGTICQFIV 540

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           Q++ S  +G GVGKL+AVSDPRKGG P+GF
Sbjct: 541 QNFGSPRQGGGVGKLIAVSDPRKGGAPAGF 570

BLAST of MC01g_new0374 vs. ExPASy TrEMBL
Match: A0A6J1BVV4 (gamma-glutamyltranspeptidase 1 OS=Momordica charantia OX=3673 GN=LOC111005973 PE=4 SV=1)

HSP 1 Score: 1109 bits (2868), Expect = 0.0
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV
Sbjct: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA
Sbjct: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE
Sbjct: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS
Sbjct: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR
Sbjct: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP
Sbjct: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL
Sbjct: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL
Sbjct: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV
Sbjct: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF
Sbjct: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570

BLAST of MC01g_new0374 vs. ExPASy TrEMBL
Match: A0A0A0KGC3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G501260 PE=4 SV=1)

HSP 1 Score: 985 bits (2547), Expect = 0.0
Identity = 502/570 (88.07%), Postives = 533/570 (93.51%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           ML+RWL+IVLVS  LL PT IARPEV VASHGAVAADD RCS+IGM+VLREGGHAVDASV
Sbjct: 18  MLIRWLVIVLVSIGLLTPTSIARPEVVVASHGAVAADDRRCSKIGMDVLREGGHAVDASV 77

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVSPASSGIGGGAFMLIRSA G +QA DMRETAP LASENMYAGN ASKA+GA
Sbjct: 78  AAALCLGVVSPASSGIGGGAFMLIRSASGKAQAFDMRETAPLLASENMYAGNVASKASGA 137

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSI VPGELAGLHEAWKQHG+L WARLVQPAERLARLGFKISPYLHMQMVRTE GILGDE
Sbjct: 138 LSIAVPGELAGLHEAWKQHGKLPWARLVQPAERLARLGFKISPYLHMQMVRTESGILGDE 197

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNL TSGGKLLK GD+VRN+KLA+TLRTISRFGV AFYNGSIGINLIRDIRKLGG+LS
Sbjct: 198 GLRNLLTSGGKLLKQGDIVRNQKLAETLRTISRFGVSAFYNGSIGINLIRDIRKLGGLLS 257

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSYQVK RVPIL+RTM LE+ TMPPPSGGSGVILMLNILAQYK PSGL GPLGIHR
Sbjct: 258 MKDLQSYQVKTRVPILSRTMDLEITTMPPPSGGSGVILMLNILAQYKLPSGLLGPLGIHR 317

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHV+AVRTNLGDP F+NV+EVLS+MLSPKFA+QLKKTI DN+TF PSHYGGRWNP
Sbjct: 318 EIEALKHVVAVRTNLGDPDFYNVTEVLSDMLSPKFAEQLKKTINDNKTFHPSHYGGRWNP 377

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           INDHGTSHLS+VD ERNAVSMTTTVN YFGAHILSPSTGIVLNNEMDDF++PANVSAN+L
Sbjct: 378 INDHGTSHLSVVDGERNAVSMTTTVNYYFGAHILSPSTGIVLNNEMDDFAIPANVSANQL 437

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAPANFIRPGKRPLSSMSPTIVLK+G+LK VVGASGGGFIIAGTAEVLLNHFAKGM P 
Sbjct: 438 PPAPANFIRPGKRPLSSMSPTIVLKNGQLKAVVGASGGGFIIAGTAEVLLNHFAKGMSPF 497

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYH L+PN++NYENWTTVLGDHFEVPA+TRAVLAKKGHVLEGLAGGTICQFIV
Sbjct: 498 SSVMAPRVYHQLVPNILNYENWTTVLGDHFEVPAETRAVLAKKGHVLEGLAGGTICQFIV 557

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           Q  SSL +G G GKLVAVSDPRKGG P+GF
Sbjct: 558 QGSSSLRQGDGFGKLVAVSDPRKGGAPAGF 587

BLAST of MC01g_new0374 vs. ExPASy TrEMBL
Match: A0A6J1KR70 (glutathione hydrolase 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496901 PE=4 SV=1)

HSP 1 Score: 980 bits (2534), Expect = 0.0
Identity = 502/570 (88.07%), Postives = 535/570 (93.86%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           MLMRWLIIVLVS ALL PT IARPEV VASHGAVAADD RCSRIGM+VLREGGHAVDAS+
Sbjct: 1   MLMRWLIIVLVSIALLIPTSIARPEVIVASHGAVAADDSRCSRIGMDVLREGGHAVDASI 60

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVS ASSGIGGGAFMLIRSA G SQA DMRETAP LA EN+YA N ASKAAGA
Sbjct: 61  AAALCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENIYASNVASKAAGA 120

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSI VPGELAGLHEAWKQ+G+L WARLVQPAERLARLGFKISPYLHMQMV+TE GILGDE
Sbjct: 121 LSIAVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDE 180

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNLFTSGGKLLKPGDV+RNKKLA+TLRTISRFGV+AFYNGSIGINLIRDIRKLGGILS
Sbjct: 181 GLRNLFTSGGKLLKPGDVIRNKKLAQTLRTISRFGVQAFYNGSIGINLIRDIRKLGGILS 240

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSY+VK+RVPI ARTMG E+ITMPPPSGGSG+ILMLNILAQYK PSGL GPLGIHR
Sbjct: 241 MKDLQSYRVKIRVPISARTMGHEIITMPPPSGGSGLILMLNILAQYKLPSGLLGPLGIHR 300

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHV AVRTNLGDP+F+NVS+VLS+MLSPKFA++LKKTI DNRTFDPSHYGGRWNP
Sbjct: 301 EIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGRWNP 360

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           I+DHGTSHLS+VD ERNAVSMTTTVN YFGA ILSPSTGI+LNNEMDDFSVPANVSANKL
Sbjct: 361 IHDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSANKL 420

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAP+NFIRPGKRPLSSMSP IVLK+G+LKGVVGASGGGFIIAGTAEV+LNHFAKGMDP 
Sbjct: 421 PPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGMDPF 480

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYH LIPNV+ YENWTTVLGDHFEVPA+TRA LAKKGHVLEGLAGGTICQFIV
Sbjct: 481 SSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAGGTICQFIV 540

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           Q++ S  +G GVGKL+AVSDPRKGG P+GF
Sbjct: 541 QNFGSPRQGGGVGKLIAVSDPRKGGAPAGF 570

BLAST of MC01g_new0374 vs. ExPASy TrEMBL
Match: A0A6J1KLV6 (glutathione hydrolase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496901 PE=4 SV=1)

HSP 1 Score: 980 bits (2534), Expect = 0.0
Identity = 502/570 (88.07%), Postives = 535/570 (93.86%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           MLMRWLIIVLVS ALL PT IARPEV VASHGAVAADD RCSRIGM+VLREGGHAVDAS+
Sbjct: 4   MLMRWLIIVLVSIALLIPTSIARPEVIVASHGAVAADDSRCSRIGMDVLREGGHAVDASI 63

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVS ASSGIGGGAFMLIRSA G SQA DMRETAP LA EN+YA N ASKAAGA
Sbjct: 64  AAALCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENIYASNVASKAAGA 123

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSI VPGELAGLHEAWKQ+G+L WARLVQPAERLARLGFKISPYLHMQMV+TE GILGDE
Sbjct: 124 LSIAVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDE 183

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNLFTSGGKLLKPGDV+RNKKLA+TLRTISRFGV+AFYNGSIGINLIRDIRKLGGILS
Sbjct: 184 GLRNLFTSGGKLLKPGDVIRNKKLAQTLRTISRFGVQAFYNGSIGINLIRDIRKLGGILS 243

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSY+VK+RVPI ARTMG E+ITMPPPSGGSG+ILMLNILAQYK PSGL GPLGIHR
Sbjct: 244 MKDLQSYRVKIRVPISARTMGHEIITMPPPSGGSGLILMLNILAQYKLPSGLLGPLGIHR 303

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHV AVRTNLGDP+F+NVS+VLS+MLSPKFA++LKKTI DNRTFDPSHYGGRWNP
Sbjct: 304 EIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGRWNP 363

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           I+DHGTSHLS+VD ERNAVSMTTTVN YFGA ILSPSTGI+LNNEMDDFSVPANVSANKL
Sbjct: 364 IHDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSANKL 423

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAP+NFIRPGKRPLSSMSP IVLK+G+LKGVVGASGGGFIIAGTAEV+LNHFAKGMDP 
Sbjct: 424 PPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGMDPF 483

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYH LIPNV+ YENWTTVLGDHFEVPA+TRA LAKKGHVLEGLAGGTICQFIV
Sbjct: 484 SSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAGGTICQFIV 543

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           Q++ S  +G GVGKL+AVSDPRKGG P+GF
Sbjct: 544 QNFGSPRQGGGVGKLIAVSDPRKGGAPAGF 573

BLAST of MC01g_new0374 vs. ExPASy TrEMBL
Match: A0A6J1KR64 (glutathione hydrolase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496901 PE=4 SV=1)

HSP 1 Score: 980 bits (2534), Expect = 0.0
Identity = 502/570 (88.07%), Postives = 535/570 (93.86%), Query Frame = 0

Query: 1   MLMRWLIIVLVSTALLPPTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 60
           MLMRWLIIVLVS ALL PT IARPEV VASHGAVAADD RCSRIGM+VLREGGHAVDAS+
Sbjct: 16  MLMRWLIIVLVSIALLIPTSIARPEVIVASHGAVAADDSRCSRIGMDVLREGGHAVDASI 75

Query: 61  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 120
           AAALCLGVVS ASSGIGGGAFMLIRSA G SQA DMRETAP LA EN+YA N ASKAAGA
Sbjct: 76  AAALCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENIYASNVASKAAGA 135

Query: 121 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 180
           LSI VPGELAGLHEAWKQ+G+L WARLVQPAERLARLGFKISPYLHMQMV+TE GILGDE
Sbjct: 136 LSIAVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDE 195

Query: 181 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 240
           GLRNLFTSGGKLLKPGDV+RNKKLA+TLRTISRFGV+AFYNGSIGINLIRDIRKLGGILS
Sbjct: 196 GLRNLFTSGGKLLKPGDVIRNKKLAQTLRTISRFGVQAFYNGSIGINLIRDIRKLGGILS 255

Query: 241 MKDLQSYQVKLRVPILARTMGLELITMPPPSGGSGVILMLNILAQYKPPSGLSGPLGIHR 300
           MKDLQSY+VK+RVPI ARTMG E+ITMPPPSGGSG+ILMLNILAQYK PSGL GPLGIHR
Sbjct: 256 MKDLQSYRVKIRVPISARTMGHEIITMPPPSGGSGLILMLNILAQYKLPSGLLGPLGIHR 315

Query: 301 EIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNP 360
           EIEALKHV AVRTNLGDP+F+NVS+VLS+MLSPKFA++LKKTI DNRTFDPSHYGGRWNP
Sbjct: 316 EIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGRWNP 375

Query: 361 INDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKL 420
           I+DHGTSHLS+VD ERNAVSMTTTVN YFGA ILSPSTGI+LNNEMDDFSVPANVSANKL
Sbjct: 376 IHDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSANKL 435

Query: 421 PPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPL 480
           PPAP+NFIRPGKRPLSSMSP IVLK+G+LKGVVGASGGGFIIAGTAEV+LNHFAKGMDP 
Sbjct: 436 PPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGMDPF 495

Query: 481 SSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIV 540
           SSVMAPRVYH LIPNV+ YENWTTVLGDHFEVPA+TRA LAKKGHVLEGLAGGTICQFIV
Sbjct: 496 SSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAGGTICQFIV 555

Query: 541 QDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 570
           Q++ S  +G GVGKL+AVSDPRKGG P+GF
Sbjct: 556 QNFGSPRQGGGVGKLIAVSDPRKGGAPAGF 585

BLAST of MC01g_new0374 vs. TAIR 10
Match: AT4G39640.1 (gamma-glutamyl transpeptidase 1 )

HSP 1 Score: 716.5 bits (1848), Expect = 1.7e-206
Identity = 376/573 (65.62%), Postives = 443/573 (77.31%), Query Frame = 0

Query: 2   LMRWLIIVLVSTALLPPTFI-ARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 61
           L+R + IVL   A L       R +  V S GAVA DDGRCS IGM VLREGG+A+DASV
Sbjct: 3   LVRTVTIVLFIIAFLQNAAAQKRQQSIVKSRGAVATDDGRCSVIGMRVLREGGNAIDASV 62

Query: 62  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 121
           AAALCLGVVSPASSGIGGGAF +++ A G   A D RETAP  A+ENMY GN   K  GA
Sbjct: 63  AAALCLGVVSPASSGIGGGAFTVVKIAGGKEIAYDSRETAPLRATENMYGGNVDLKKKGA 122

Query: 122 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 181
           LS+GVPGE+AGL  AWKQHG+L W RLV PAE+LA  GFKIS YL+MQM  T   IL D+
Sbjct: 123 LSVGVPGEVAGLFTAWKQHGKLPWKRLVTPAEKLAE-GFKISKYLYMQMNATRSDILADK 182

Query: 182 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 241
           GL +LF S G+L KPG +  N KLA TL+ I  +G KAFYNG++G+NL RDI+K GGI++
Sbjct: 183 GLSDLFVSNGELKKPGTICHNPKLALTLKLIGEYGPKAFYNGTVGVNLARDIKKSGGIIT 242

Query: 242 MKDLQSYQVKLRVPILARTMGLELITMPPP-SGGSGVILMLNILAQYKPPSGLSGPLGIH 301
           +KDLQSY+VK++ P+ A  +G  ++ MPPP SGG+ ++L+LNIL+QY  PSG+SGPLG+H
Sbjct: 243 LKDLQSYRVKIKEPLSADILGYRVLGMPPPSSGGAAMMLVLNILSQYGIPSGVSGPLGVH 302

Query: 302 REIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWN 361
           R IEALKH  AVR NLGDP F +V++V+S+MLSPKFA+ LK  I D +TFDP +YGG WN
Sbjct: 303 RLIEALKHAFAVRMNLGDPDFTDVTKVVSDMLSPKFAKDLKSKINDQKTFDPKYYGGMWN 362

Query: 362 PINDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVP--ANVSA 421
            I+DHGTSHLSI+D ERNAVSMT+T+N YFGA +LSPSTGIVLNNEMDDFS+P  +N + 
Sbjct: 363 QIDDHGTSHLSIIDRERNAVSMTSTINGYFGALMLSPSTGIVLNNEMDDFSIPMKSNGNL 422

Query: 422 NKLPPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGM 481
           +  PPAPANFIRPGKRPLSSMSPTIVLKDGK+K  VGASGG  IIAGT EV LNHF   M
Sbjct: 423 DVPPPAPANFIRPGKRPLSSMSPTIVLKDGKVKAAVGASGGANIIAGTTEVYLNHFFLKM 482

Query: 482 DPLSSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQ 541
           DPLSSV+APR+YH LIPN  +YENWTTV  DHFE+P  TR VL KKGHVL  +AGGTI Q
Sbjct: 483 DPLSSVLAPRIYHQLIPNRASYENWTTVFNDHFEIPKATRVVLEKKGHVLSPIAGGTIAQ 542

Query: 542 FIVQDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 571
           FIVQ+ S    G G  +LVAVSDPRKGG PSG+
Sbjct: 543 FIVQE-SGENSG-GRSELVAVSDPRKGGFPSGY 572

BLAST of MC01g_new0374 vs. TAIR 10
Match: AT4G39640.2 (gamma-glutamyl transpeptidase 1 )

HSP 1 Score: 716.5 bits (1848), Expect = 1.7e-206
Identity = 376/573 (65.62%), Postives = 443/573 (77.31%), Query Frame = 0

Query: 2   LMRWLIIVLVSTALLPPTFI-ARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 61
           L+R + IVL   A L       R +  V S GAVA DDGRCS IGM VLREGG+A+DASV
Sbjct: 3   LVRTVTIVLFIIAFLQNAAAQKRQQSIVKSRGAVATDDGRCSVIGMRVLREGGNAIDASV 62

Query: 62  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 121
           AAALCLGVVSPASSGIGGGAF +++ A G   A D RETAP  A+ENMY GN   K  GA
Sbjct: 63  AAALCLGVVSPASSGIGGGAFTVVKIAGGKEIAYDSRETAPLRATENMYGGNVDLKKKGA 122

Query: 122 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 181
           LS+GVPGE+AGL  AWKQHG+L W RLV PAE+LA  GFKIS YL+MQM  T   IL D+
Sbjct: 123 LSVGVPGEVAGLFTAWKQHGKLPWKRLVTPAEKLAE-GFKISKYLYMQMNATRSDILADK 182

Query: 182 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 241
           GL +LF S G+L KPG +  N KLA TL+ I  +G KAFYNG++G+NL RDI+K GGI++
Sbjct: 183 GLSDLFVSNGELKKPGTICHNPKLALTLKLIGEYGPKAFYNGTVGVNLARDIKKSGGIIT 242

Query: 242 MKDLQSYQVKLRVPILARTMGLELITMPPP-SGGSGVILMLNILAQYKPPSGLSGPLGIH 301
           +KDLQSY+VK++ P+ A  +G  ++ MPPP SGG+ ++L+LNIL+QY  PSG+SGPLG+H
Sbjct: 243 LKDLQSYRVKIKEPLSADILGYRVLGMPPPSSGGAAMMLVLNILSQYGIPSGVSGPLGVH 302

Query: 302 REIEALKHVIAVRTNLGDPSFFNVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWN 361
           R IEALKH  AVR NLGDP F +V++V+S+MLSPKFA+ LK  I D +TFDP +YGG WN
Sbjct: 303 RLIEALKHAFAVRMNLGDPDFTDVTKVVSDMLSPKFAKDLKSKINDQKTFDPKYYGGMWN 362

Query: 362 PINDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVP--ANVSA 421
            I+DHGTSHLSI+D ERNAVSMT+T+N YFGA +LSPSTGIVLNNEMDDFS+P  +N + 
Sbjct: 363 QIDDHGTSHLSIIDRERNAVSMTSTINGYFGALMLSPSTGIVLNNEMDDFSIPMKSNGNL 422

Query: 422 NKLPPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGM 481
           +  PPAPANFIRPGKRPLSSMSPTIVLKDGK+K  VGASGG  IIAGT EV LNHF   M
Sbjct: 423 DVPPPAPANFIRPGKRPLSSMSPTIVLKDGKVKAAVGASGGANIIAGTTEVYLNHFFLKM 482

Query: 482 DPLSSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQ 541
           DPLSSV+APR+YH LIPN  +YENWTTV  DHFE+P  TR VL KKGHVL  +AGGTI Q
Sbjct: 483 DPLSSVLAPRIYHQLIPNRASYENWTTVFNDHFEIPKATRVVLEKKGHVLSPIAGGTIAQ 542

Query: 542 FIVQDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 571
           FIVQ+ S    G G  +LVAVSDPRKGG PSG+
Sbjct: 543 FIVQE-SGENSG-GRSELVAVSDPRKGGFPSGY 572

BLAST of MC01g_new0374 vs. TAIR 10
Match: AT4G39650.1 (gamma-glutamyl transpeptidase 2 )

HSP 1 Score: 698.4 bits (1801), Expect = 4.9e-201
Identity = 361/574 (62.89%), Postives = 434/574 (75.61%), Query Frame = 0

Query: 2   LMRWLIIVLVSTALLP-PTFIARPEVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASV 61
           L+R   I L+  A L     +   +  VA HGAVA DDGRCS IG  VLR+GG+A+DASV
Sbjct: 7   LVRTATIALLLIAFLQNANAVKNLQSIVAYHGAVATDDGRCSAIGTNVLRQGGNAIDASV 66

Query: 62  AAALCLGVVSPASSGIGGGAFMLIRSADGHSQALDMRETAPFLASENMYAGNAASKAAGA 121
           AAALCLGVVSPASSGIGGGAF +I+ A+G   A D RETAP  A+E+MY  N   K  G+
Sbjct: 67  AAALCLGVVSPASSGIGGGAFTMIKLANGTEVAYDSRETAPLSATEDMYGDNPERKKKGS 126

Query: 122 LSIGVPGELAGLHEAWKQHGRLSWARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDE 181
           LS+GVPGE+AGL+ AW QHG+L W +LV+PAE+LA  GFKIS YL+MQM  T   IL D+
Sbjct: 127 LSVGVPGEVAGLYTAWTQHGKLPWKQLVEPAEKLAAEGFKISKYLYMQMNATRSDILADK 186

Query: 182 GLRNLFTSGGKLLKPGDVVRNKKLAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILS 241
           GL  LF S G+L KPG + RN KLA TL  I+ +G KAFYNG++G NL+ DI+K GGI++
Sbjct: 187 GLSELFVSNGELKKPGAICRNPKLADTLSQIAEYGPKAFYNGTVGFNLVSDIQKAGGIIT 246

Query: 242 MKDLQSYQVKLRVPILARTMGLELITMPPP-SGGSGVILMLNILAQYKPPSGLSGPLGIH 301
           +KDLQ+Y VK++ P+    +G  L+ MPPP SGG  ++L+LNILAQY  PSG+SGPLG+H
Sbjct: 247 LKDLQNYNVKVKEPLSTEILGYRLLGMPPPSSGGPAMMLILNILAQYGIPSGVSGPLGVH 306

Query: 302 REIEALKHVIAVRTNLGDPSFF-NVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRW 361
           R +EALKH  AVR NLGDP F   V+ V+++MLSPKFAQ LK  I D +TFDP +YGG+W
Sbjct: 307 RLVEALKHAFAVRMNLGDPDFVPEVTNVVADMLSPKFAQDLKSKINDEKTFDPKYYGGKW 366

Query: 362 NPINDHGTSHLSIVDAERNAVSMTTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSAN 421
             I DHGTSHLSI+D+ERNAVSMT+T+N YFGA +LSPSTGIVLNNEMDDFS+P     +
Sbjct: 367 GQIKDHGTSHLSIIDSERNAVSMTSTINGYFGAIMLSPSTGIVLNNEMDDFSIPTKSGGD 426

Query: 422 K--LPPAPANFIRPGKRPLSSMSPTIVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKG 481
               PPAPANFIRPGKRPLSSM+PTIVLKDGK+K  +GASGG +IIAGT +V LNHF   
Sbjct: 427 PDVPPPAPANFIRPGKRPLSSMTPTIVLKDGKVKAALGASGGMYIIAGTTQVYLNHFFLN 486

Query: 482 MDPLSSVMAPRVYHPLIPNVVNYENWTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTIC 541
           MDPLSSV+APR+YH LIPN  +YENWTTV  DHFE+P + R VL KKG VL  +AGGTI 
Sbjct: 487 MDPLSSVVAPRIYHQLIPNKASYENWTTVYSDHFEIPEEIRLVLEKKGQVLTPIAGGTIS 546

Query: 542 QFIVQDYSSLGKGVGVGKLVAVSDPRKGGVPSGF 571
           Q IV+   S GK  G+ KLVAVSDPRKGG PSG+
Sbjct: 547 QLIVE--QSDGKSGGISKLVAVSDPRKGGFPSGY 578

BLAST of MC01g_new0374 vs. TAIR 10
Match: AT4G29210.1 (gamma-glutamyl transpeptidase 4 )

HSP 1 Score: 548.9 bits (1413), Expect = 4.8e-156
Identity = 284/562 (50.53%), Postives = 385/562 (68.51%), Query Frame = 0

Query: 25  EVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASVAAALCLGVVSPASSGIGGGAFMLI 84
           +V  + +G VAADD RCS IG  VLR GGHAVDA+VA  LC+GVV+P SSGIGGG+F+++
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRSGGHAVDAAVAITLCVGVVNPMSSGIGGGSFLIV 133

Query: 85  RS-ADGHSQALDMRETAPFLASENMYAGNAASKAAGALSIGVPGELAGLHEAWKQHGRLS 144
            S  D  ++A DMRETAP  AS++MY  +A++K+ GALS+GVPGE+AGL+EAWK++GRL 
Sbjct: 134 SSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRLP 193

Query: 145 WARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDEGLRNLFTSGGKLLKPGDVVRNKK 204
           W  L +PA +LAR GF + PYL   +      IL D G+R++F+  G++LK G+   N +
Sbjct: 194 WKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSRNGQVLKTGETCYNPE 253

Query: 205 LAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILSMKDLQSYQVKLRVPILARTMGLE 264
           LA++L TIS  G  AFYNG++G  L++D++K GGI++M DL+SY+V++   +    MG  
Sbjct: 254 LAQSLETISEQGPGAFYNGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAMSVDVMGYT 313

Query: 265 LITMPPPSGGS-GVILMLNILAQYKPPSGLSG-PLGIHREIEALKHVIAVRTNLGDPSFF 324
           +  MPPPSGG+ G  +++NIL  Y      SG  LG+HR IEA+KH+ A R +LGDP F 
Sbjct: 314 VHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMDLGDPEFV 373

Query: 325 NVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNPINDHGTSHLSIVDAERNAVSM 384
           NV+  ++ MLS   A++++K I+DN TF P +Y  RW+ + D GTSH  +VDA+RN+VSM
Sbjct: 374 NVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDADRNSVSM 433

Query: 385 TTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKLPPAPANFIRPGKRPLSSMSPT 444
           T+TVN  FGA +LSPSTGIVLNNEMDDFS P  ++ + LPPAP NFI P KRPLSSM+P 
Sbjct: 434 TSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRPLSSMTPL 493

Query: 445 IVLKDGKLKGVVGASGGGFIIAGTAEVLLNHFAKGMDPLSSVMAPRVYHPLIPNVVNYEN 504
           ++ KDG+    +G +GG  II    +V LN F   M P  +V + R+YH LIPNVV+YEN
Sbjct: 494 VITKDGEFVAALGGAGGMHIIPAVLQVFLNCFVLNMKPKEAVESARIYHRLIPNVVSYEN 553

Query: 505 WTTVLGDHFEVPAKTRAVLAKKGHVLEGLAGGTICQFIVQDYS---------SLGKGVG- 564
           +TT+ GDH  V   T+  LA++GH L+ L+GG I Q IVQ +           +G+ +G 
Sbjct: 554 FTTINGDHIGVSEDTKMFLAERGHELKELSGGAIVQLIVQSFKEEKEEEMIIEIGRKIGK 613

Query: 565 -----VGKLVAVSDPRKGGVPS 569
                 G L AVSDPRK G P+
Sbjct: 614 KSKPLKGLLTAVSDPRKDGKPA 635

BLAST of MC01g_new0374 vs. TAIR 10
Match: AT4G29210.2 (gamma-glutamyl transpeptidase 4 )

HSP 1 Score: 446.8 bits (1148), Expect = 2.6e-125
Identity = 225/424 (53.07%), Postives = 306/424 (72.17%), Query Frame = 0

Query: 25  EVTVASHGAVAADDGRCSRIGMEVLREGGHAVDASVAAALCLGVVSPASSGIGGGAFMLI 84
           +V  + +G VAADD RCS IG  VLR GGHAVDA+VA  LC+GVV+P SSGIGGG+F+++
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRSGGHAVDAAVAITLCVGVVNPMSSGIGGGSFLIV 133

Query: 85  RS-ADGHSQALDMRETAPFLASENMYAGNAASKAAGALSIGVPGELAGLHEAWKQHGRLS 144
            S  D  ++A DMRETAP  AS++MY  +A++K+ GALS+GVPGE+AGL+EAWK++GRL 
Sbjct: 134 SSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRLP 193

Query: 145 WARLVQPAERLARLGFKISPYLHMQMVRTEPGILGDEGLRNLFTSGGKLLKPGDVVRNKK 204
           W  L +PA +LAR GF + PYL   +      IL D G+R++F+  G++LK G+   N +
Sbjct: 194 WKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSRNGQVLKTGETCYNPE 253

Query: 205 LAKTLRTISRFGVKAFYNGSIGINLIRDIRKLGGILSMKDLQSYQVKLRVPILARTMGLE 264
           LA++L TIS  G  AFYNG++G  L++D++K GGI++M DL+SY+V++   +    MG  
Sbjct: 254 LAQSLETISEQGPGAFYNGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAMSVDVMGYT 313

Query: 265 LITMPPPSGGS-GVILMLNILAQYKPPSGLSG-PLGIHREIEALKHVIAVRTNLGDPSFF 324
           +  MPPPSGG+ G  +++NIL  Y      SG  LG+HR IEA+KH+ A R +LGDP F 
Sbjct: 314 VHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMDLGDPEFV 373

Query: 325 NVSEVLSNMLSPKFAQQLKKTIYDNRTFDPSHYGGRWNPINDHGTSHLSIVDAERNAVSM 384
           NV+  ++ MLS   A++++K I+DN TF P +Y  RW+ + D GTSH  +VDA+RN+VSM
Sbjct: 374 NVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDADRNSVSM 433

Query: 385 TTTVNSYFGAHILSPSTGIVLNNEMDDFSVPANVSANKLPPAPANFIRPGKRPLSSMSPT 444
           T+TVN  FGA +LSPSTGIVLNNEMDDFS P  ++ + LPPAP NFI P KRPLSSM+P 
Sbjct: 434 TSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRPLSSMTPL 493

Query: 445 IVLK 446
           ++ K
Sbjct: 494 VITK 497