MC01g_new0315 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0315
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	WAT1-related protein
Location	MC01: 13385271 .. 13387946 (+)
RNA-Seq Expression	MC01g_new0315
Synteny	MC01g_new0315

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTACTCAAACAACAACACACACAGCAGAAGCAAGAAAAGAGAATTGGGATTCATCAGAATGGAGTCAAAAACCAAACCTTATTTTGCTGCCATTATCTTCCAAATCATTTTTGCTGGGATGAGTATTTTGTCAAAAGCTGCCTTTTCTTATGGTATGAACTCTTATATTTTCATTTTCTATAGGCAAGCTCTTGGAGCTCTCCTCCTACTGCCTCCTACCATCATTTTCAGAAGGTAAAAACAAAATTTAAATTAGGGTTCATAAGTTAACTATATTTAATTCCTCTCTCTTTTAACCTATTTTTTTGCTTTCCTTTTGCCTGGATTATTATTGTAGAAAAGAAGTAAGATGTTTATCATTTGGGGAGGTGTGCAAGATTTTCATGCTGGCCTTTCTGGGGTTTGTACAATTTTTTAAGTTTTTTTATTCTCTGAATTAATTGAACATCTTTTTATGGTATCATCTATTGTAAATAGTATTTAATTTATTTTATGTATACAAAAATTGCAGGAGAACGCTGACTTTGAATGCTAATGGAATAGGCGTTAATTACACATCTGCAAATTTGGGCTCGGCAGCATTTAACTGTGTTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTTAGGTACTAAAATTGAACTGAAATCAATAGTGATATGCAAATATATCTGATTGTTTGCTTAACAATTTTATTTTTAGTGTGACTTTTAAAAAGCATTGAACTCAGTAGACCATAATTCCCTACCACATGATTTTCAATCTTTTTAGAAGAAGAGGTACCATATTTCGTTAAAAGTATGTGAGAGACATACGTACCAAAGAGGAGACCTAGATGAAAGCACTCAAATTGAGAGAAATAGTAGATATATTGTAATTACAAAATAGACTTGATTTTGAGGACCATGTAGATGCAGAGACAATAATATCCTCTTATAAACTATTTAGAGATTTATAAATGTCCCCAAAGATTCGGTTGTTTCTCTCCAACCAAATATTCCAAAGGATGGCACCAATAGCTCCATGATTTTCAATTTTAAAAAGTAGAGAGAAAACATGTTACCTTTTTCTTGTTTAAAAAATGATAATCATACCCTTTTGATCTTCTGGGAAGTCCTCAATGATACACAAAATGTATTGGGGTGCCCACTGCCCATATTTAATTTGAAACTTTACTATTCATCACATGTTAGAATGTACCTAGTGATCTAGTGTAACTGTAACCGAGAGTATAGAAACATCAACCATACCTTTTTTTTTTTTTTTTTTGATAACGGGGAGCAATACCCAGGGATACCCACACCCATCTAAGGCCCACATCTGAGAGACATCAAAAGTTTTTGGTATTAAACTCACCAGAAGGTTCGAATCTAAGACCTCTAAGCCAATATATGACTAAGAGACTCTTGAGCTTTTGTCAAACTGAACTGTTACTTGGGGCTTACAAGAACACCTTTAATAACTTTCAAAACCTCACCTTCACACGCATGTGTCGCTACACCATTTGGAGTAGTCAAAGAGAGTACTCAAAAGAAATATTTAACTAAGATTTCTCTTCAAAGTTTTGGATTTTACATGTTCCTAGCTCACTGTTTTACAACCTAGATCTATATTCATCACCTGACTATGATCCTATTTATAAATTATAATGTAAGATATATTTGTTTGGCTTAACTCAACCCATCAAGGCTCCTATAATTACTATGTTGACAGGATGGAGAAAGTAAACTTGAGGAAGGCAAGTGGGATGGCAAAGGTTGGAGGCATAATGCTGTGCATTGCAGGAGTTGCAGTACTTGCATTTTACAAAGGCCCTTATATGAAGCCATTCTTCAATTATCACCTTTTTCAAACCCACCAAAAATCTCATCTTTCTTCCAACAAAACATGGATTATTGGCTGTTTTATGCACCTCATAACCTCCATTTTACTTGGTCTCTGGTTTGTCCTTCAGGTAATAAAATAAACCCTTTAATTTAGTTTCTCATCAAATTGCAACTTCTATATCTGCCACTGTTCTATATACACATTCTCAGGCTTTGGTAATGGAGACTTGCCCGTCTCCACTTGTGTTAACGTGTGGACAAACCTTGTCAAGTGCGATTCAGTCATTTGTTGTGGCCATTGCAGTGGAGAGAAACCCTTCTGAATGGAAGCTGGGTTGGAACATTAGCCTCATTTCTATTCTTTATTGTGTATGGATCTCTCTCTTTTTCTCTCTTCTAATCTTTCATTTTGCAAAACCTACATTGCAATGGCCTACAACTTACTACAGGGAATTATTGTGAACTGCATTGGAAACTATCTATCATGTTGGGTCATCAAGAAGAAGGGTCCAGTTTTCCAAGCTGTGACCACACCCTTGAATCTGGTTATGACAATAGTTGGCTCCCAATTTCTTTTAGACGATGGAGTTAGCTTGGGAAGGTAAAAGCTAGTTTGGCTTTTAAGTACTTTATTATTGCATTATTTCATGAAACTCTTGTTTGATAAAATAATCAAGACTATATTTATAATAATTTGTAAAAGAAGGAAAAGGGAAATAGATTGTTAGGACTATTTGGATGTCATTTTATATATATAGGAAGGAATAATGATTTTTTACTAATTATTATATGTGAACCTCTTGTTAAATTTCTTTTGTTAAATTATACATTCTGTTCTTATGATTTG

mRNA sequence

ATGGAGTCAAAAACCAAACCTTATTTTGCTGCCATTATCTTCCAAATCATTTTTGCTGGGATGAGTATTTTGTCAAAAGCTGCCTTTTCTTATGGTATGAACTCTTATATTTTCATTTTCTATAGGCAAGCTCTTGGAGCTCTCCTCCTACTGCCTCCTACCATCATTTTCAGAAGAAAAGAAGTAAGATGTTTATCATTTGGGGAGGTGTGCAAGATTTTCATGCTGGCCTTTCTGGGGAGAACGCTGACTTTGAATGCTAATGGAATAGGCGTTAATTACACATCTGCAAATTTGGGCTCGGCAGCATTTAACTGTGTTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTTAGGATGGAGAAAGTAAACTTGAGGAAGGCAAGTGGGATGGCAAAGGTTGGAGGCATAATGCTGTGCATTGCAGGAGTTGCAGTACTTGCATTTTACAAAGGCCCTTATATGAAGCCATTCTTCAATTATCACCTTTTTCAAACCCACCAAAAATCTCATCTTTCTTCCAACAAAACATGGATTATTGGCTGTTTTATGCACCTCATAACCTCCATTTTACTTGGTCTCTGGTTTGTCCTTCAGGCTTTGGTAATGGAGACTTGCCCGTCTCCACTTGTGTTAACGTGTGGACAAACCTTGTCAAGTGCGATTCAGTCATTTGTTGTGGCCATTGCAGTGGAGAGAAACCCTTCTGAATGGAAGCTGGGTTGGAACATTAGCCTCATTTCTATTCTTTATTGTGGAATTATTGTGAACTGCATTGGAAACTATCTATCATGTTGGGTCATCAAGAAGAAGGGTCCAGTTTTCCAAGCTGTGACCACACCCTTGAATCTGGTTATGACAATAGTTGGCTCCCAATTTCTTTTAGACGATGGAGTTAGCTTGGGAAGGTAA

Coding sequence (CDS)

ATGGAGTCAAAAACCAAACCTTATTTTGCTGCCATTATCTTCCAAATCATTTTTGCTGGGATGAGTATTTTGTCAAAAGCTGCCTTTTCTTATGGTATGAACTCTTATATTTTCATTTTCTATAGGCAAGCTCTTGGAGCTCTCCTCCTACTGCCTCCTACCATCATTTTCAGAAGAAAAGAAGTAAGATGTTTATCATTTGGGGAGGTGTGCAAGATTTTCATGCTGGCCTTTCTGGGGAGAACGCTGACTTTGAATGCTAATGGAATAGGCGTTAATTACACATCTGCAAATTTGGGCTCGGCAGCATTTAACTGTGTTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTTAGGATGGAGAAAGTAAACTTGAGGAAGGCAAGTGGGATGGCAAAGGTTGGAGGCATAATGCTGTGCATTGCAGGAGTTGCAGTACTTGCATTTTACAAAGGCCCTTATATGAAGCCATTCTTCAATTATCACCTTTTTCAAACCCACCAAAAATCTCATCTTTCTTCCAACAAAACATGGATTATTGGCTGTTTTATGCACCTCATAACCTCCATTTTACTTGGTCTCTGGTTTGTCCTTCAGGCTTTGGTAATGGAGACTTGCCCGTCTCCACTTGTGTTAACGTGTGGACAAACCTTGTCAAGTGCGATTCAGTCATTTGTTGTGGCCATTGCAGTGGAGAGAAACCCTTCTGAATGGAAGCTGGGTTGGAACATTAGCCTCATTTCTATTCTTTATTGTGGAATTATTGTGAACTGCATTGGAAACTATCTATCATGTTGGGTCATCAAGAAGAAGGGTCCAGTTTTCCAAGCTGTGACCACACCCTTGAATCTGGTTATGACAATAGTTGGCTCCCAATTTCTTTTAGACGATGGAGTTAGCTTGGGAAGGTAA

Protein sequence

MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRKEVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSLGR

Homology

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BLAST of MC01g_new0315 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 6.7e-69
Identity = 141/309 (45.63%), Postives = 199/309 (64.40%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           MESK KPY    I Q+I+  M ++SKA F+ GMN+++F+FYRQA   + L P    F RK
Sbjct: 3   MESK-KPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERK 62

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
               LSF    KIFML+  G TL+L+ NGI ++YTSA L +A    +P  TFF A +F M
Sbjct: 63  SAPPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGM 122

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMK----PFFNYHLFQTHQKS--HLSS 180
           E++ ++   G AK+ GI +C+ GV +LA YKGP +K    P F +     H+ +  H+S 
Sbjct: 123 ERLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHVSG 182

Query: 181 NKT-WIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVER 240
             T W+ GC + + ++IL GLW VLQ  V++  PS L  T    L S+IQSFV+AIA+ER
Sbjct: 183 GSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALER 242

Query: 241 NPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQF 300
           + S WKLGWN+ L++++YCG IV  +  YL  WVI+K+GPVF ++ TPL+L+ T++ S  
Sbjct: 243 DISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAI 302

Query: 301 LLDDGVSLG 303
           LL + +SLG
Sbjct: 303 LLCEIISLG 310

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BLAST of MC01g_new0315 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 9.8e-52
Identity = 120/302 (39.74%), Postives = 171/302 (56.62%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           M  + K   A +  QI++AGM +LSK A S G N ++F+FYRQA  AL L P        
Sbjct: 1   MMMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESS 60

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
           +   LSF  + KIF ++  G TL+LN   + +  T+A   +A  N +P  TF  A +FR+
Sbjct: 61  KSSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRL 120

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180
           E V L+K+ G+AKV G M+ + G  V AF KGP +    N++   T     + S K  + 
Sbjct: 121 ETVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL---INHYNSSTIPNGTVPSTKNSVK 180

Query: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240
           G    L  +    LW ++Q+ VM+  P+ L L   Q L S IQS V A+AV RNPS WK+
Sbjct: 181 GSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKI 240

Query: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300
            + + L+S+ YCGI+V  +  +L  W I+KKGPVF A+ TPL L++T + S FL  +   
Sbjct: 241 EFGLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFY 299

Query: 301 LG 303
           LG
Sbjct: 301 LG 299

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BLAST of MC01g_new0315 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.4e-42
Identity = 104/301 (34.55%), Postives = 166/301 (55.15%), Query Frame = 0


Query: 4   KTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRKEVR 63
           K +PY   I  Q   AG  I+  A  + G N Y+ I YR  + AL+L P  +IF RK   
Sbjct: 10  KLRPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFALIFERKVRP 69

Query: 64  CLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKV 123
            ++   + KI  L FL   L      +G+N TSA   SA  N +P  TF  A+I RMEKV
Sbjct: 70  KMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFIIAWILRMEKV 129

Query: 124 NLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMK-PFFNYHLFQTHQKSHLS-SNKTWIIG 183
           N+ +    AK+ G ++ + G  V+  YKGP +  P+ N ++ Q +  ++ S  +  W++G
Sbjct: 130 NIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQDHNNWVVG 189

Query: 184 CFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLG 243
             + L+  +    ++VLQ++ ++T P+ L L+    L+ A+QSF VA+ VER+PS W +G
Sbjct: 190 TLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSGWAVG 249

Query: 244 WNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSL 303
           W+  L + LY GI+ + I  Y+   V+K +GPVF     PL +++  + + F+L + +  
Sbjct: 250 WDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHF 309

[Close]

BLAST of MC01g_new0315 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.4e-42
Identity = 98/308 (31.82%), Postives = 168/308 (54.55%), Query Frame = 0


Query: 3   SKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRKEV 62
           + +KPYFA I  Q  +AGM+I++K + + GM+ Y+ + YR A+   ++ P    F RK  
Sbjct: 14  TSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQ 73

Query: 63  RCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEK 122
             ++F    ++F+L  LG  +  N   +G+ YTS     A  N +P  TF  A +FRME 
Sbjct: 74  PKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEM 133

Query: 123 VNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLF-------QTHQKSHLSSN 182
           ++L+K    AK+ G ++ +AG  ++  YKGP ++ F+  ++         T    + SS+
Sbjct: 134 LDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSD 193

Query: 183 KTWIIGCFMHLITSILLGLWFVLQALVMET-CPSPLVLTCGQTLSSAIQSFVVAIAVERN 242
           K ++ G  + +  ++     FVLQA +++T     L LT        +Q+  V   +E N
Sbjct: 194 KEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHN 253

Query: 243 PSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFL 302
           PS W++GW+++L++  Y GI+ + I  Y+   V+KK+GPVF    +PL +V+  V   F+
Sbjct: 254 PSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFV 313

[Close]

BLAST of MC01g_new0315 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.8e-38
Identity = 98/302 (32.45%), Postives = 161/302 (53.31%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           M    KPYFA +  Q  +AGM++++K     GM+ Y+ + YR A     + P  ++  RK
Sbjct: 5   MSESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERK 64

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
               ++F    +IF+LA LG  +  N   IG+  TS    SA  N VP  T   A +FRM
Sbjct: 65  VRSKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRM 124

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180
           EKV +RK   + KV G ++ + G  ++ FYKGP++  FF  HL  T   S  +++  ++ 
Sbjct: 125 EKVEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFIN-FFRSHL--TAASSPPTAD--YLK 184

Query: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240
                L+ S+    +FVLQA  ++   + L ++        +QS  +A  +E NPS   +
Sbjct: 185 AAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNI 244

Query: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300
           G++++L++  Y GI+ + I  Y+   ++++KGPVF     PL +V+  + S F+L  G+ 
Sbjct: 245 GFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIY 301

Query: 301 LG 303
           LG
Sbjct: 305 LG 301

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BLAST of MC01g_new0315 vs. NCBI nr
Match: XP_022132849.1 (WAT1-related protein At5g64700-like isoform X1 [Momordica charantia])

HSP 1 Score: 599 bits (1545), Expect = 1.06e-214
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK
Sbjct: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
           EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM
Sbjct: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180
           EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII
Sbjct: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180

Query: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240
           GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL
Sbjct: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240

Query: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300
           GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS
Sbjct: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300

Query: 301 LG 302
           LG
Sbjct: 301 LG 302

[Close]

BLAST of MC01g_new0315 vs. NCBI nr
Match: XP_022132853.1 (WAT1-related protein At5g64700-like [Momordica charantia])

HSP 1 Score: 541 bits (1395), Expect = 2.51e-192
Identity = 274/303 (90.43%), Postives = 287/303 (94.72%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           MESKTKPYFAAIIFQI FAGMSILSKAAF+ GMN+YIFIFYRQALG LL LP TIIF+RK
Sbjct: 3   MESKTKPYFAAIIFQITFAGMSILSKAAFASGMNTYIFIFYRQALGTLLQLPLTIIFKRK 62

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
           EV CLS GEVCKIFMLAFLGRTLTLNANGIGVNYTSA LGSAAFN VPVTTFFFAFIFRM
Sbjct: 63  EVSCLSVGEVCKIFMLAFLGRTLTLNANGIGVNYTSATLGSAAFNIVPVTTFFFAFIFRM 122

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLS-SNKTWI 180
           EKVN+RKASGMAKVGGI+LCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLS SNKTWI
Sbjct: 123 EKVNMRKASGMAKVGGILLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSNSNKTWI 182

Query: 181 IGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWK 240
           IGCFM LI SIL+G WFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPS+WK
Sbjct: 183 IGCFMLLIASILIGFWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSQWK 242

Query: 241 LGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGV 300
           LGW+I LI++LYCGI+V CIGNYLSCWVIK+KGPVFQAVTTPLNLVMT+VGSQFLL DGV
Sbjct: 243 LGWDIRLIAVLYCGIVVICIGNYLSCWVIKEKGPVFQAVTTPLNLVMTLVGSQFLLSDGV 302

Query: 301 SLG 302
           SLG
Sbjct: 303 SLG 305

[Close]

BLAST of MC01g_new0315 vs. NCBI nr
Match: XP_022132850.1 (WAT1-related protein At5g64700-like isoform X2 [Momordica charantia])

HSP 1 Score: 521 bits (1342), Expect = 2.65e-184
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 0


Query: 43  QALGALLLLPPTIIFRRKEVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSA 102
           QALGALLLLPPTIIFRRKEVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSA
Sbjct: 10  QALGALLLLPPTIIFRRKEVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSA 69

Query: 103 AFNCVPVTTFFFAFIFRMEKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYH 162
           AFNCVPVTTFFFAFIFRMEKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYH
Sbjct: 70  AFNCVPVTTFFFAFIFRMEKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYH 129

Query: 163 LFQTHQKSHLSSNKTWIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAI 222
           LFQTHQKSHLSSNKTWIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAI
Sbjct: 130 LFQTHQKSHLSSNKTWIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAI 189

Query: 223 QSFVVAIAVERNPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPL 282
           QSFVVAIAVERNPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPL
Sbjct: 190 QSFVVAIAVERNPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPL 249

Query: 283 NLVMTIVGSQFLLDDGVSLG 302
           NLVMTIVGSQFLLDDGVSLG
Sbjct: 250 NLVMTIVGSQFLLDDGVSLG 269

[Close]

BLAST of MC01g_new0315 vs. NCBI nr
Match: XP_022132854.1 (WAT1-related protein At5g64700-like isoform X1 [Momordica charantia])

HSP 1 Score: 464 bits (1195), Expect = 5.64e-162
Identity = 234/302 (77.48%), Postives = 262/302 (86.75%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           MESK +PYFAAI+ QIIF+GMS+LSKAAF+ GMN+Y+F+FYRQA G+L LLP TI F+RK
Sbjct: 1   MESK-RPYFAAIVSQIIFSGMSLLSKAAFASGMNTYVFLFYRQAFGSLFLLPLTIFFKRK 60

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
           EV  LS GE+ K FM A LG TL LN  GIGVNYTSA LG+AAFNC+PVTTF FA I RM
Sbjct: 61  EVTSLSLGEMWKNFMQASLGFTLALNTYGIGVNYTSATLGAAAFNCLPVTTFLFALILRM 120

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180
           EKVN+RK SGMAKVGGI+LCIAGVAVLAFYKGPYMKP FNYHLFQTHQKSHLSS+KTWII
Sbjct: 121 EKVNIRKTSGMAKVGGIVLCIAGVAVLAFYKGPYMKPLFNYHLFQTHQKSHLSSHKTWII 180

Query: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240
           GCF  L++ I  GLW VLQALV++TCPSPLVLTC QTLSSAIQSFVVAIAVERNPS+WKL
Sbjct: 181 GCFFLLVSCISWGLWIVLQALVLKTCPSPLVLTCVQTLSSAIQSFVVAIAVERNPSQWKL 240

Query: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300
           GWNI LI++LYCG  V CI N LS WVIKKKGPVFQAV TPLNL+ T++GS+FLL+DGVS
Sbjct: 241 GWNIRLIAVLYCGTFVICIANSLSSWVIKKKGPVFQAVNTPLNLIFTLIGSEFLLNDGVS 300

Query: 301 LG 302
           LG
Sbjct: 301 LG 301

[Close]

BLAST of MC01g_new0315 vs. NCBI nr
Match: XP_022132851.1 (WAT1-related protein At5g64700-like isoform X3 [Momordica charantia] >XP_022132852.1 WAT1-related protein At5g64700-like isoform X3 [Momordica charantia])

HSP 1 Score: 458 bits (1178), Expect = 5.61e-160
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 75  MLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKVNLRKASGMAKV 134
           MLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKVNLRKASGMAKV
Sbjct: 1   MLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKVNLRKASGMAKV 60

Query: 135 GGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWIIGCFMHLITSILLGL 194
           GGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWIIGCFMHLITSILLGL
Sbjct: 61  GGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWIIGCFMHLITSILLGL 120

Query: 195 WFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLGWNISLISILYCGI 254
           WFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLGWNISLISILYCGI
Sbjct: 121 WFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLGWNISLISILYCGI 180

Query: 255 IVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSLG 302
           IVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSLG
Sbjct: 181 IVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSLG 228

[Close]

BLAST of MC01g_new0315 vs. ExPASy TrEMBL
Match: A0A6J1BU92 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005599 PE=3 SV=1)

HSP 1 Score: 599 bits (1545), Expect = 5.15e-215
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK
Sbjct: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
           EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM
Sbjct: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180
           EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII
Sbjct: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180

Query: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240
           GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL
Sbjct: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240

Query: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300
           GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS
Sbjct: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300

Query: 301 LG 302
           LG
Sbjct: 301 LG 302

[Close]

BLAST of MC01g_new0315 vs. ExPASy TrEMBL
Match: A0A6J1BXG5 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005600 PE=3 SV=1)

HSP 1 Score: 541 bits (1395), Expect = 1.21e-192
Identity = 274/303 (90.43%), Postives = 287/303 (94.72%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           MESKTKPYFAAIIFQI FAGMSILSKAAF+ GMN+YIFIFYRQALG LL LP TIIF+RK
Sbjct: 3   MESKTKPYFAAIIFQITFAGMSILSKAAFASGMNTYIFIFYRQALGTLLQLPLTIIFKRK 62

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
           EV CLS GEVCKIFMLAFLGRTLTLNANGIGVNYTSA LGSAAFN VPVTTFFFAFIFRM
Sbjct: 63  EVSCLSVGEVCKIFMLAFLGRTLTLNANGIGVNYTSATLGSAAFNIVPVTTFFFAFIFRM 122

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLS-SNKTWI 180
           EKVN+RKASGMAKVGGI+LCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLS SNKTWI
Sbjct: 123 EKVNMRKASGMAKVGGILLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSNSNKTWI 182

Query: 181 IGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWK 240
           IGCFM LI SIL+G WFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPS+WK
Sbjct: 183 IGCFMLLIASILIGFWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSQWK 242

Query: 241 LGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGV 300
           LGW+I LI++LYCGI+V CIGNYLSCWVIK+KGPVFQAVTTPLNLVMT+VGSQFLL DGV
Sbjct: 243 LGWDIRLIAVLYCGIVVICIGNYLSCWVIKEKGPVFQAVTTPLNLVMTLVGSQFLLSDGV 302

Query: 301 SLG 302
           SLG
Sbjct: 303 SLG 305

[Close]

BLAST of MC01g_new0315 vs. ExPASy TrEMBL
Match: A0A6J1BTL4 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005599 PE=3 SV=1)

HSP 1 Score: 521 bits (1342), Expect = 1.28e-184
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 0


Query: 43  QALGALLLLPPTIIFRRKEVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSA 102
           QALGALLLLPPTIIFRRKEVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSA
Sbjct: 10  QALGALLLLPPTIIFRRKEVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSA 69

Query: 103 AFNCVPVTTFFFAFIFRMEKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYH 162
           AFNCVPVTTFFFAFIFRMEKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYH
Sbjct: 70  AFNCVPVTTFFFAFIFRMEKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYH 129

Query: 163 LFQTHQKSHLSSNKTWIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAI 222
           LFQTHQKSHLSSNKTWIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAI
Sbjct: 130 LFQTHQKSHLSSNKTWIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAI 189

Query: 223 QSFVVAIAVERNPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPL 282
           QSFVVAIAVERNPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPL
Sbjct: 190 QSFVVAIAVERNPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPL 249

Query: 283 NLVMTIVGSQFLLDDGVSLG 302
           NLVMTIVGSQFLLDDGVSLG
Sbjct: 250 NLVMTIVGSQFLLDDGVSLG 269

[Close]

BLAST of MC01g_new0315 vs. ExPASy TrEMBL
Match: A0A6J1BU97 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005601 PE=3 SV=1)

HSP 1 Score: 464 bits (1195), Expect = 2.73e-162
Identity = 234/302 (77.48%), Postives = 262/302 (86.75%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           MESK +PYFAAI+ QIIF+GMS+LSKAAF+ GMN+Y+F+FYRQA G+L LLP TI F+RK
Sbjct: 1   MESK-RPYFAAIVSQIIFSGMSLLSKAAFASGMNTYVFLFYRQAFGSLFLLPLTIFFKRK 60

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
           EV  LS GE+ K FM A LG TL LN  GIGVNYTSA LG+AAFNC+PVTTF FA I RM
Sbjct: 61  EVTSLSLGEMWKNFMQASLGFTLALNTYGIGVNYTSATLGAAAFNCLPVTTFLFALILRM 120

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180
           EKVN+RK SGMAKVGGI+LCIAGVAVLAFYKGPYMKP FNYHLFQTHQKSHLSS+KTWII
Sbjct: 121 EKVNIRKTSGMAKVGGIVLCIAGVAVLAFYKGPYMKPLFNYHLFQTHQKSHLSSHKTWII 180

Query: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240
           GCF  L++ I  GLW VLQALV++TCPSPLVLTC QTLSSAIQSFVVAIAVERNPS+WKL
Sbjct: 181 GCFFLLVSCISWGLWIVLQALVLKTCPSPLVLTCVQTLSSAIQSFVVAIAVERNPSQWKL 240

Query: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300
           GWNI LI++LYCG  V CI N LS WVIKKKGPVFQAV TPLNL+ T++GS+FLL+DGVS
Sbjct: 241 GWNIRLIAVLYCGTFVICIANSLSSWVIKKKGPVFQAVNTPLNLIFTLIGSEFLLNDGVS 300

Query: 301 LG 302
           LG
Sbjct: 301 LG 301

[Close]

BLAST of MC01g_new0315 vs. ExPASy TrEMBL
Match: A0A6J1BV00 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005599 PE=3 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 2.72e-160
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 75  MLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKVNLRKASGMAKV 134
           MLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKVNLRKASGMAKV
Sbjct: 1   MLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKVNLRKASGMAKV 60

Query: 135 GGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWIIGCFMHLITSILLGL 194
           GGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWIIGCFMHLITSILLGL
Sbjct: 61  GGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWIIGCFMHLITSILLGL 120

Query: 195 WFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLGWNISLISILYCGI 254
           WFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLGWNISLISILYCGI
Sbjct: 121 WFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLGWNISLISILYCGI 180

Query: 255 IVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSLG 302
           IVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSLG
Sbjct: 181 IVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSLG 228

[Close]

BLAST of MC01g_new0315 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 262.3 bits (669), Expect = 4.8e-70
Identity = 141/309 (45.63%), Postives = 199/309 (64.40%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           MESK KPY    I Q+I+  M ++SKA F+ GMN+++F+FYRQA   + L P    F RK
Sbjct: 3   MESK-KPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERK 62

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
               LSF    KIFML+  G TL+L+ NGI ++YTSA L +A    +P  TFF A +F M
Sbjct: 63  SAPPLSFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGM 122

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMK----PFFNYHLFQTHQKS--HLSS 180
           E++ ++   G AK+ GI +C+ GV +LA YKGP +K    P F +     H+ +  H+S 
Sbjct: 123 ERLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHVSG 182

Query: 181 NKT-WIIGCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVER 240
             T W+ GC + + ++IL GLW VLQ  V++  PS L  T    L S+IQSFV+AIA+ER
Sbjct: 183 GSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALER 242

Query: 241 NPSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQF 300
           + S WKLGWN+ L++++YCG IV  +  YL  WVI+K+GPVF ++ TPL+L+ T++ S  
Sbjct: 243 DISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAI 302

Query: 301 LLDDGVSLG 303
           LL + +SLG
Sbjct: 303 LLCEIISLG 310

[Close]

BLAST of MC01g_new0315 vs. TAIR 10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 205.3 bits (521), Expect = 6.9e-53
Identity = 120/302 (39.74%), Postives = 171/302 (56.62%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           M  + K   A +  QI++AGM +LSK A S G N ++F+FYRQA  AL L P        
Sbjct: 1   MMMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESS 60

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
           +   LSF  + KIF ++  G TL+LN   + +  T+A   +A  N +P  TF  A +FR+
Sbjct: 61  KSSPLSFILLLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRL 120

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180
           E V L+K+ G+AKV G M+ + G  V AF KGP +    N++   T     + S K  + 
Sbjct: 121 ETVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL---INHYNSSTIPNGTVPSTKNSVK 180

Query: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240
           G    L  +    LW ++Q+ VM+  P+ L L   Q L S IQS V A+AV RNPS WK+
Sbjct: 181 GSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKI 240

Query: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300
            + + L+S+ YCGI+V  +  +L  W I+KKGPVF A+ TPL L++T + S FL  +   
Sbjct: 241 EFGLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFY 299

Query: 301 LG 303
           LG
Sbjct: 301 LG 299

[Close]

BLAST of MC01g_new0315 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.0e-43
Identity = 104/301 (34.55%), Postives = 166/301 (55.15%), Query Frame = 0


Query: 4   KTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRKEVR 63
           K +PY   I  Q   AG  I+  A  + G N Y+ I YR  + AL+L P  +IF RK   
Sbjct: 10  KLRPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFALIFERKVRP 69

Query: 64  CLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEKV 123
            ++   + KI  L FL   L      +G+N TSA   SA  N +P  TF  A+I RMEKV
Sbjct: 70  KMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFIIAWILRMEKV 129

Query: 124 NLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMK-PFFNYHLFQTHQKSHLS-SNKTWIIG 183
           N+ +    AK+ G ++ + G  V+  YKGP +  P+ N ++ Q +  ++ S  +  W++G
Sbjct: 130 NIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQDHNNWVVG 189

Query: 184 CFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKLG 243
             + L+  +    ++VLQ++ ++T P+ L L+    L+ A+QSF VA+ VER+PS W +G
Sbjct: 190 TLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSGWAVG 249

Query: 244 WNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVSL 303
           W+  L + LY GI+ + I  Y+   V+K +GPVF     PL +++  + + F+L + +  
Sbjct: 250 WDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHF 309

[Close]

BLAST of MC01g_new0315 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.0e-43
Identity = 98/308 (31.82%), Postives = 168/308 (54.55%), Query Frame = 0


Query: 3   SKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRKEV 62
           + +KPYFA I  Q  +AGM+I++K + + GM+ Y+ + YR A+   ++ P    F RK  
Sbjct: 14  TSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQ 73

Query: 63  RCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRMEK 122
             ++F    ++F+L  LG  +  N   +G+ YTS     A  N +P  TF  A +FRME 
Sbjct: 74  PKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEM 133

Query: 123 VNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLF-------QTHQKSHLSSN 182
           ++L+K    AK+ G ++ +AG  ++  YKGP ++ F+  ++         T    + SS+
Sbjct: 134 LDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSD 193

Query: 183 KTWIIGCFMHLITSILLGLWFVLQALVMET-CPSPLVLTCGQTLSSAIQSFVVAIAVERN 242
           K ++ G  + +  ++     FVLQA +++T     L LT        +Q+  V   +E N
Sbjct: 194 KEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHN 253

Query: 243 PSEWKLGWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFL 302
           PS W++GW+++L++  Y GI+ + I  Y+   V+KK+GPVF    +PL +V+  V   F+
Sbjct: 254 PSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFV 313

[Close]

BLAST of MC01g_new0315 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 160.6 bits (405), Expect = 2.0e-39
Identity = 98/302 (32.45%), Postives = 161/302 (53.31%), Query Frame = 0


Query: 1   MESKTKPYFAAIIFQIIFAGMSILSKAAFSYGMNSYIFIFYRQALGALLLLPPTIIFRRK 60
           M    KPYFA +  Q  +AGM++++K     GM+ Y+ + YR A     + P  ++  RK
Sbjct: 5   MSESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERK 64

Query: 61  EVRCLSFGEVCKIFMLAFLGRTLTLNANGIGVNYTSANLGSAAFNCVPVTTFFFAFIFRM 120
               ++F    +IF+LA LG  +  N   IG+  TS    SA  N VP  T   A +FRM
Sbjct: 65  VRSKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRM 124

Query: 121 EKVNLRKASGMAKVGGIMLCIAGVAVLAFYKGPYMKPFFNYHLFQTHQKSHLSSNKTWII 180
           EKV +RK   + KV G ++ + G  ++ FYKGP++  FF  HL  T   S  +++  ++ 
Sbjct: 125 EKVEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFIN-FFRSHL--TAASSPPTAD--YLK 184

Query: 181 GCFMHLITSILLGLWFVLQALVMETCPSPLVLTCGQTLSSAIQSFVVAIAVERNPSEWKL 240
                L+ S+    +FVLQA  ++   + L ++        +QS  +A  +E NPS   +
Sbjct: 185 AAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNI 244

Query: 241 GWNISLISILYCGIIVNCIGNYLSCWVIKKKGPVFQAVTTPLNLVMTIVGSQFLLDDGVS 300
           G++++L++  Y GI+ + I  Y+   ++++KGPVF     PL +V+  + S F+L  G+ 
Sbjct: 245 GFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIY 301

Query: 301 LG 303
           LG
Sbjct: 305 LG 301

The following BLAST results are available for this feature:

BLAST of MC01g_new0315 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGG3	6.7e-69	45.63	WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2...	[more]
Q6NMB7	9.8e-52	39.74	WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2...	[more]
Q9SUF1	1.4e-42	34.55	WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2...	[more]
Q9FL41	1.4e-42	31.82	WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2...	[more]
F4IJ08	2.8e-38	32.45	WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2...	[more]

BLAST of MC01g_new0315 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132849.1	1.06e-214	100.00	WAT1-related protein At5g64700-like isoform X1 [Momordica charantia]	[more]
XP_022132853.1	2.51e-192	90.43	WAT1-related protein At5g64700-like [Momordica charantia]	[more]
XP_022132850.1	2.65e-184	100.00	WAT1-related protein At5g64700-like isoform X2 [Momordica charantia]	[more]
XP_022132854.1	5.64e-162	77.48	WAT1-related protein At5g64700-like isoform X1 [Momordica charantia]	[more]
XP_022132851.1	5.61e-160	100.00	WAT1-related protein At5g64700-like isoform X3 [Momordica charantia] >XP_0221328...	[more]

BLAST of MC01g_new0315 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BU92	5.15e-215	100.00	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005599 PE=3 SV=1	[more]
A0A6J1BXG5	1.21e-192	90.43	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005600 PE=3 SV=1	[more]
A0A6J1BTL4	1.28e-184	100.00	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005599 PE=3 SV=1	[more]
A0A6J1BU97	2.73e-162	77.48	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005601 PE=3 SV=1	[more]
A0A6J1BV00	2.72e-160	100.00	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111005599 PE=3 SV=1	[more]

BLAST of MC01g_new0315 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64700.1	4.8e-70	45.63	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G43650.1	6.9e-53	39.74	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G08290.1	1.0e-43	34.55	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G07050.1	1.0e-43	31.82	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G40900.1	2.0e-39	32.45	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 180..296 e-value: 9.6E-7 score: 29.0 coord: 6..147 e-value: 9.0E-11 score: 42.1
None	No IPR available	PANTHER	PTHR31218:SF166	WAT1-RELATED PROTEIN	coord: 6..302
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 41..151
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 6..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g_new0315.1	MC01g_new0315.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity