MC01g_new0307 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0307 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.2e-101
Identity = 186/354 (52.54%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLG-----QGSARYHHRHFHLRPTRLFVFGDSYVDTGNINV 60
           MDS   LF  +F     SLL G     +GS + HH  +  RPT+LFVFGDSY DTGNI  
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHL-YPFRPTKLFVFGDSYADTGNIKK 60

Query: 61  NTSSARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGIN 120
             SS+   PYGITFPG P+GR SDGRV TD+LA++ G+KSP+P+      G KR + G+N
Sbjct: 61  AFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQYGMN 120

Query: 121 FAFGGTGIFDTSFPFPNMTTQINFFQKLI-ANSIFTLNDIHSSIALVSVSGNDYSFYQAT 180
           FA+GGTG+F+T  P PNMTTQI+ FQ ++    I+   ++ SS+ALVSV+GNDYS + A 
Sbjct: 121 FAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIAL 180

Query: 181 NGSMEGFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATM 240
           N     F  FI  VV+Q  +NL+RIH LGV+K+ +  L PLGCLP  T  +SF +CN T 
Sbjct: 181 NRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQ 240

Query: 241 DSLVEFHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPC 300
           ++LV  HN LL+Q V KLN ETK S F ILD+Y   L++ +NKGS      RFE+PLKPC
Sbjct: 241 NALVNLHNNLLQQVVAKLNNETKQSTFIILDLYNAFLTVFKNKGS-NPGSTRFESPLKPC 300

Query: 301 CFGVSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNL 349
           C GVS E++CGSVD+KG KKY +C +PK A FWD +HPT+EGW   +  L  +L
Sbjct: 301 CVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLRESL 352

BLAST of MC01g_new0307 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.8e-97
Identity = 180/354 (50.85%), Postives = 235/354 (66.38%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHF-HLRPTRLFVFGDSYVDTGNINVNTSS 60
           M+  KLL SL F  S  S  LG  S    H +   LRP RLFVFGDSY DTGNI  + S 
Sbjct: 1   MELPKLLISL-FLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSD 60

Query: 61  ARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFG 120
           +  IPYGITFP  PSGR SDGRV TD+LARY G+KSP+P+T     G +R   G+N+A+G
Sbjct: 61  SWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYG 120

Query: 121 GTGIFDT-SFPFPNMTTQINFFQK-LIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGS 180
           GTG+F T   P PNMTTQI++FQ+ L A +I++ +D+ SS+ALVSV+GNDY+ + A    
Sbjct: 121 GTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRP 180

Query: 181 MEGFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSL 240
           +     F+  VV+QIA+N  RIH LGV K+V+  + PLGCLP +T  +SF +CNAT ++ 
Sbjct: 181 LTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNAS 240

Query: 241 VEFHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFG 300
              HN LL +A+ +LN ETK S F +LD Y   L++ +NKG P     RF  PLKPCC G
Sbjct: 241 TNLHNYLLHKAIARLNNETKPSTFVVLDHYNAFLTVFKNKG-PEPGVSRFGNPLKPCCVG 300

Query: 301 VSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 352
           V+S + C +VD+KG KKY +C+DPK A FWD  HP++EGW   +  L  +LK +
Sbjct: 301 VNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLKAI 352

BLAST of MC01g_new0307 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 270.4 bits (690), Expect = 2.9e-71
Identity = 154/352 (43.75%), Postives = 212/352 (60.23%), Query Frame = 0

Query: 7   LFSLIFSLSLFSLLLGQ-GSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSARNIPY 66
           L  L FSLSLF  + G  GS +    +   +  +LFVFG+SY DTGN+   T+ +  +PY
Sbjct: 4   LMKLFFSLSLFFGINGAVGSNQPSQAN---KNPKLFVFGNSYADTGNMK-PTALSWKLPY 63

Query: 67  GITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPR--NGINFAFGGTGI 126
           GITFPG PSGR SDG   TD+LA+  G K P     W+  G K+ +   G+NFAFGG+ +
Sbjct: 64  GITFPGKPSGRYSDGLTATDFLAKQLGAKLPY---LWRTHGKKKVKLNRGMNFAFGGSEV 123

Query: 127 FDTSFP-FPNMTTQINFFQKL-IANSIFTLN-DIHSSIALVSVSGNDYSFYQATNGSMEG 186
           FD+     PN++TQ+ F   L +A  ++T++ D+ SS AL+S SG DY  +   N +M  
Sbjct: 124 FDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAA 183

Query: 187 FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF 246
           +  F+  +V  I  +L  ++GL  + + +  L PLGCLP +T ASSF  CN +   LV  
Sbjct: 184 YPAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRL 243

Query: 247 HNALLKQAVEKLNKE----TKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCF 306
           HN  LK+AV KLNKE    TKG  F I+D++   ++I++ KG+      RF++PLKPCC 
Sbjct: 244 HNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILEKKGN-----KRFKSPLKPCCE 303

Query: 307 GVSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNL 349
           G      C  +D KG KKYTLC DPK A FWD ++PTQEGW   +  LG +L
Sbjct: 304 G-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLLGKSL 338

BLAST of MC01g_new0307 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.0e-68
Identity = 143/324 (44.14%), Postives = 195/324 (60.19%), Query Frame = 0

Query: 35  LRP---TRLFVFGDSYVDTGNINVNTSSARNIPYGITFPGVPSGRLSDGRVLTDYLARYF 94
           LRP    +LFVFGDSY DTGN   +T  A  IPYGITFPG PSGR  DG + TD+L +  
Sbjct: 7   LRPRKVPKLFVFGDSYADTGNTKRDT-EAWAIPYGITFPGKPSGRYCDGLIATDFLEKVL 66

Query: 95  GLKSPVPFTT-WQNVGVKRPRNGINFAFGGTGIFDTS--FPFPNMTTQINFFQKLIANSI 154
           G +SP  + T  ++ G+KR   G+NFAFGG+ + D+S   PFPN+T Q+NF   L+    
Sbjct: 67  GAESPYLYRTHGRDKGLKR---GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGR 126

Query: 155 FTLNDIHSSIALVSVSGNDYSFYQATNGSMEGFKPFIVSVVNQIAINLKRIHGLGVRKVV 214
              +   S ++L+S +G DY +Y   N    G K  +  VV+ + +N+  + GL  +K+ 
Sbjct: 127 VYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIA 186

Query: 215 MMGLGPLGCLPELTAASSFSKCNATMDSLVEFHNALLKQAVEKLNKET----KGSPFFIL 274
           +  L P+GCLP  T+ASSF  CN +  +LVE HN LLK+ V KLN+++    K   FFI+
Sbjct: 187 VTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFII 246

Query: 275 DIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSSEFSCGSVDKKGNKKYTLCKDPKLA 334
           DI+   +++++NKGS      RF+ P+K CC G      CG     G K YTLC DPK  
Sbjct: 247 DIHNAFMTVMKNKGS-----KRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDPKSF 306

Query: 335 LFWDSVHPTQEGWFVAFRSLGSNL 349
            FWD+VHPTQEGW   +  LG+ L
Sbjct: 307 FFWDAVHPTQEGWRSIYSVLGNPL 315

BLAST of MC01g_new0307 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 245.4 bits (625), Expect = 9.9e-64
Identity = 135/320 (42.19%), Postives = 182/320 (56.88%), Query Frame = 0

Query: 33  FHLRPTRLFVFGDSYVDTGNINVNTSSARNIPYGITFPGVPSGRLSDGRVLTDYLARYFG 92
           F  +PT+LFVFGDSY DTGN       +   P GITFPG+P+GR SDGRV TDYLA+Y G
Sbjct: 41  FGFKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIG 100

Query: 93  LKSPVPFTTWQNVGVKRPR----NGINFAFGGTGIFDTSFPF-PNMTTQINFFQKLIANS 152
           +++P+ +  W   G  RPR     G+NFA+GG G F+T F   P  + QI+ F++L+  +
Sbjct: 101 VRTPITY-KWGKYG--RPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRN 160

Query: 153 IFTLNDIHSSIALVSVSGNDYSFYQATNGSMEGFKPFIVSVVNQIAINLKRIHGLGVRKV 212
           +++  D++SS+A  S+ GNDY  Y   NGS EG       VV QI +++KRI  LGVRKV
Sbjct: 161 VYSPADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKV 220

Query: 213 VMMGLGPLGCLPELTAASSFSKCNATMDSLVEFHNALLKQAVEKLNKE---TKGSPFFIL 272
           ++    P  CLP+L        C+ T D+    HN+LL++ + KLN +        F  L
Sbjct: 221 LVALSPPQKCLPKLVTPKG---CD-TNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTL 280

Query: 273 DIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSSEFSCGSVDKKGNKKYTLCKDPKLA 332
           D+Y   ++I +NKG        F  P K CC      F CG     G K YTLC DPK  
Sbjct: 281 DLYNAFVTIFKNKG--VSGVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSF 340

Query: 333 LFWDSVHPTQEGWFVAFRSL 345
            FWD+VH + +GW   F  L
Sbjct: 341 FFWDNVHISDQGWRSVFSLL 350

BLAST of MC01g_new0307 vs. NCBI nr
Match: XP_022133081.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 714 bits (1843), Expect = 8.65e-260
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60
           MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA
Sbjct: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60

Query: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFGG 120
           RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFGG
Sbjct: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFGG 120

Query: 121 TGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSMEG 180
           TGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSMEG
Sbjct: 121 TGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSMEG 180

Query: 181 FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF 240
           FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF
Sbjct: 181 FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF 240

Query: 241 HNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSS 300
           HNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSS
Sbjct: 241 HNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSS 300

Query: 301 EFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351
           EFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL
Sbjct: 301 EFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351

BLAST of MC01g_new0307 vs. NCBI nr
Match: XP_038883507.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 534 bits (1376), Expect = 1.06e-188
Identity = 269/353 (76.20%), Postives = 300/353 (84.99%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60
           M SKKLLF+ + SL +FSLL GQGSA     HFH+ PT+L VFGDSYVDTGNINVNTS A
Sbjct: 2   MASKKLLFTFL-SLLIFSLLSGQGSAG----HFHIHPTKLIVFGDSYVDTGNINVNTSGA 61

Query: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLK-SPVPFTTWQNVGVKRPRNGINFAFG 120
           RN PYGITFPGVPSGR SDGRVLTD+ A+Y GLK SP PF  WQ +G KRP+ GINFAFG
Sbjct: 62  RNFPYGITFPGVPSGRFSDGRVLTDFFAKYLGLKKSPTPFIAWQKIGNKRPKFGINFAFG 121

Query: 121 GTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSME 180
           GTG+FDTSFPFPNMTTQIN FQ LIANSIFT  DIHSSIALVS SGNDYSFY ATNGS +
Sbjct: 122 GTGVFDTSFPFPNMTTQINLFQNLIANSIFTSYDIHSSIALVSPSGNDYSFYLATNGSPQ 181

Query: 181 GFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVE 240
           GFKPF++SVVNQIA+NLKRI+ LGV+K+V++GLGP+GC P+LTA S F+KCNATM+S VE
Sbjct: 182 GFKPFVISVVNQIAVNLKRIYRLGVKKIVVVGLGPVGCYPQLTAPS-FNKCNATMNSFVE 241

Query: 241 FHNALLKQAVEKLNKETKGSP-FFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGV 300
           FHN LLK+AVEKLN ETKGSP FFILD+Y T+LSIIQNKG+P K    F+TPLKPCCFGV
Sbjct: 242 FHNILLKKAVEKLNNETKGSPNFFILDMYDTVLSIIQNKGNP-KVGVSFKTPLKPCCFGV 301

Query: 301 SSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351
           SSEFSCGSVD+ GNK YTLCK P LALFWDS+HPTQ+GWF AF SL SNLK L
Sbjct: 302 SSEFSCGSVDEHGNKMYTLCKHPNLALFWDSLHPTQKGWFAAFSSLRSNLKHL 347

BLAST of MC01g_new0307 vs. NCBI nr
Match: XP_022962769.1 (GDSL esterase/lipase At5g03610-like [Cucurbita moschata])

HSP 1 Score: 510 bits (1314), Expect = 3.33e-179
Identity = 251/350 (71.71%), Postives = 290/350 (82.86%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRH-FHLRPTRLFVFGDSYVDTGNINVNTSS 60
           M+S+KL+F L+ SL +FSLLLGQGS R H  + F+ RPT+LFVFGDSYVDTGNI   TSS
Sbjct: 1   MESRKLVFPLL-SLFVFSLLLGQGSGRRHVDYRFNSRPTKLFVFGDSYVDTGNIKATTSS 60

Query: 61  ARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFG 120
           +RN PYGITFPGVPSGR SDGRVLTD+ A Y  LKSP  F+ W+  G K P+ G+NFAFG
Sbjct: 61  SRNFPYGITFPGVPSGRFSDGRVLTDFFADYLELKSPETFSGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSME 180
           GTG+FDTSFPFPNMTTQI+FFQ L+AN+IF    + SS+ALVS SGNDYSFYQ  NGSME
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFAPKHVGSSVALVSPSGNDYSFYQERNGSME 180

Query: 181 GFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVE 240
           G +PFI+SVVNQIA+NLKRIH LGV+K+V++GLG +GCLP+ TA SSF  CNAT +S+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIHSLGVKKIVVLGLGAVGCLPQPTANSSFKHCNATSNSMAK 240

Query: 241 FHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVS 300
            HN LLKQAVEKLNKETKGSPF I D+Y  +LSII+NKG+P KAR RFETPLKPCCFGVS
Sbjct: 241 VHNLLLKQAVEKLNKETKGSPFIIFDVYNAILSIIRNKGNP-KARARFETPLKPCCFGVS 300

Query: 301 SEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLK 349
           SEFSCGSVD+KG K YTLCK PK ALFWDSVHPTQ+GWF  F SL S+LK
Sbjct: 301 SEFSCGSVDEKGKKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLK 348

BLAST of MC01g_new0307 vs. NCBI nr
Match: XP_023518578.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 509 bits (1310), Expect = 1.35e-178
Identity = 249/350 (71.14%), Postives = 290/350 (82.86%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRH-FHLRPTRLFVFGDSYVDTGNINVNTSS 60
           M+S+KL+F L+ SL +FSLL GQGS R H  + F+ RPT+LFVFGDSYVDTGNI  +TSS
Sbjct: 1   MESRKLVFPLL-SLFVFSLLSGQGSGRRHVDYRFNSRPTKLFVFGDSYVDTGNIKASTSS 60

Query: 61  ARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFG 120
           +RN PYGITFPGVPSGR SDGRVLTD+ A Y  LKSP  F+ W+  G K P+ G+NFAFG
Sbjct: 61  SRNFPYGITFPGVPSGRFSDGRVLTDFFANYLELKSPETFSGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSME 180
           GTG+FDTSFPFPNMTTQI+FFQ L+AN+IF    + SS+ALVS SGNDYSFYQ  NGS+E
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFAPKHVGSSVALVSPSGNDYSFYQERNGSIE 180

Query: 181 GFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVE 240
           G +PFI+SVVNQIA+NLKRIH LGV+K+V++GLGP+GCLP+ TA SSF  CNAT +S+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIHSLGVKKIVVLGLGPVGCLPQPTANSSFKHCNATSNSMAK 240

Query: 241 FHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVS 300
            HN LLKQAVEKLNKETKGSPF I D+Y  +LSII+NKG+P KAR RFETPLKPCCFGVS
Sbjct: 241 VHNLLLKQAVEKLNKETKGSPFIIFDVYNAILSIIRNKGNP-KARVRFETPLKPCCFGVS 300

Query: 301 SEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLK 349
           SEFSCGSVD+KGNK YTLC  PK  LFWDSVHPTQ+GWF  F SL S+LK
Sbjct: 301 SEFSCGSVDEKGNKMYTLCTRPKSTLFWDSVHPTQQGWFATFSSLASSLK 348

BLAST of MC01g_new0307 vs. NCBI nr
Match: XP_022972691.1 (GDSL esterase/lipase At5g03610-like [Cucurbita maxima])

HSP 1 Score: 501 bits (1291), Expect = 1.05e-175
Identity = 245/350 (70.00%), Postives = 288/350 (82.29%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRH-FHLRPTRLFVFGDSYVDTGNINVNTSS 60
           M+S+K +F L+ SL +FSLLLG+GS R+H  + F+  PT+LFVFGDSYVDTGNI    SS
Sbjct: 1   MESRKTVFPLL-SLFVFSLLLGRGSGRHHVDYRFNSWPTKLFVFGDSYVDTGNIKATNSS 60

Query: 61  ARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFG 120
           +RN+PYGITFPGVPSGR SDGRV+TD+ A Y  LKSP  F  W+  G K P+ G+NFAFG
Sbjct: 61  SRNLPYGITFPGVPSGRFSDGRVMTDFFANYLELKSPETFIGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSME 180
           GTG+FDTSFPFPNMTTQI+FFQ L+AN+IF    + SS+ALVS SGNDYSFYQ  NGSME
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFDPKHVGSSVALVSPSGNDYSFYQERNGSME 180

Query: 181 GFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVE 240
           G +PFI+SVVNQIA+NLKRI  LGV+K+V++GLGP+GCLP+ TA SSF  CN+T +S+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIQSLGVKKIVVLGLGPVGCLPQPTANSSFKHCNSTTNSMAK 240

Query: 241 FHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVS 300
            HN LLKQAVEKLNK+TKGSPF I D+Y  +LSII+NKGSP KAR RF TPLKPCCFGVS
Sbjct: 241 VHNLLLKQAVEKLNKDTKGSPFIIFDVYNAILSIIRNKGSP-KARARFVTPLKPCCFGVS 300

Query: 301 SEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLK 349
           SEFSCGSVD+KGNK YTLCK PK ALFWDSVHPTQ+GWF  F SL S+LK
Sbjct: 301 SEFSCGSVDEKGNKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLK 348

BLAST of MC01g_new0307 vs. ExPASy TrEMBL
Match: A0A6J1BU10 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111005765 PE=3 SV=1)

HSP 1 Score: 714 bits (1843), Expect = 4.19e-260
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60
           MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA
Sbjct: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60

Query: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFGG 120
           RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFGG
Sbjct: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFGG 120

Query: 121 TGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSMEG 180
           TGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSMEG
Sbjct: 121 TGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSMEG 180

Query: 181 FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF 240
           FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF
Sbjct: 181 FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF 240

Query: 241 HNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSS 300
           HNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSS
Sbjct: 241 HNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSS 300

Query: 301 EFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351
           EFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL
Sbjct: 301 EFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351

BLAST of MC01g_new0307 vs. ExPASy TrEMBL
Match: A0A6J1HI12 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111463159 PE=3 SV=1)

HSP 1 Score: 510 bits (1314), Expect = 1.61e-179
Identity = 251/350 (71.71%), Postives = 290/350 (82.86%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRH-FHLRPTRLFVFGDSYVDTGNINVNTSS 60
           M+S+KL+F L+ SL +FSLLLGQGS R H  + F+ RPT+LFVFGDSYVDTGNI   TSS
Sbjct: 1   MESRKLVFPLL-SLFVFSLLLGQGSGRRHVDYRFNSRPTKLFVFGDSYVDTGNIKATTSS 60

Query: 61  ARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFG 120
           +RN PYGITFPGVPSGR SDGRVLTD+ A Y  LKSP  F+ W+  G K P+ G+NFAFG
Sbjct: 61  SRNFPYGITFPGVPSGRFSDGRVLTDFFADYLELKSPETFSGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSME 180
           GTG+FDTSFPFPNMTTQI+FFQ L+AN+IF    + SS+ALVS SGNDYSFYQ  NGSME
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFAPKHVGSSVALVSPSGNDYSFYQERNGSME 180

Query: 181 GFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVE 240
           G +PFI+SVVNQIA+NLKRIH LGV+K+V++GLG +GCLP+ TA SSF  CNAT +S+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIHSLGVKKIVVLGLGAVGCLPQPTANSSFKHCNATSNSMAK 240

Query: 241 FHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVS 300
            HN LLKQAVEKLNKETKGSPF I D+Y  +LSII+NKG+P KAR RFETPLKPCCFGVS
Sbjct: 241 VHNLLLKQAVEKLNKETKGSPFIIFDVYNAILSIIRNKGNP-KARARFETPLKPCCFGVS 300

Query: 301 SEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLK 349
           SEFSCGSVD+KG K YTLCK PK ALFWDSVHPTQ+GWF  F SL S+LK
Sbjct: 301 SEFSCGSVDEKGKKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLK 348

BLAST of MC01g_new0307 vs. ExPASy TrEMBL
Match: A0A6J1I6N6 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111471216 PE=3 SV=1)

HSP 1 Score: 501 bits (1291), Expect = 5.09e-176
Identity = 245/350 (70.00%), Postives = 288/350 (82.29%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRH-FHLRPTRLFVFGDSYVDTGNINVNTSS 60
           M+S+K +F L+ SL +FSLLLG+GS R+H  + F+  PT+LFVFGDSYVDTGNI    SS
Sbjct: 1   MESRKTVFPLL-SLFVFSLLLGRGSGRHHVDYRFNSWPTKLFVFGDSYVDTGNIKATNSS 60

Query: 61  ARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFG 120
           +RN+PYGITFPGVPSGR SDGRV+TD+ A Y  LKSP  F  W+  G K P+ G+NFAFG
Sbjct: 61  SRNLPYGITFPGVPSGRFSDGRVMTDFFANYLELKSPETFIGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSME 180
           GTG+FDTSFPFPNMTTQI+FFQ L+AN+IF    + SS+ALVS SGNDYSFYQ  NGSME
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFDPKHVGSSVALVSPSGNDYSFYQERNGSME 180

Query: 181 GFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVE 240
           G +PFI+SVVNQIA+NLKRI  LGV+K+V++GLGP+GCLP+ TA SSF  CN+T +S+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIQSLGVKKIVVLGLGPVGCLPQPTANSSFKHCNSTTNSMAK 240

Query: 241 FHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVS 300
            HN LLKQAVEKLNK+TKGSPF I D+Y  +LSII+NKGSP KAR RF TPLKPCCFGVS
Sbjct: 241 VHNLLLKQAVEKLNKDTKGSPFIIFDVYNAILSIIRNKGSP-KARARFVTPLKPCCFGVS 300

Query: 301 SEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLK 349
           SEFSCGSVD+KGNK YTLCK PK ALFWDSVHPTQ+GWF  F SL S+LK
Sbjct: 301 SEFSCGSVDEKGNKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLK 348

BLAST of MC01g_new0307 vs. ExPASy TrEMBL
Match: A0A0A0KIM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452040 PE=3 SV=1)

HSP 1 Score: 498 bits (1281), Expect = 1.69e-174
Identity = 258/356 (72.47%), Postives = 290/356 (81.46%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60
           M S  LLFS + SL LFSL LGQGS+   H HF+  P +LFVFGDSYVDTGNINVNTSSA
Sbjct: 1   MASNNLLFSFL-SLLLFSLHLGQGSSSPVHAHFY--PLKLFVFGDSYVDTGNINVNTSSA 60

Query: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLK-SPVPFTTWQNVGVK-RPRNGINFAF 120
           RN PYGITFPG PSGR SDGRVLTD+LA Y GLK SP+PFT W+  G K R + GINFAF
Sbjct: 61  RNFPYGITFPGFPSGRFSDGRVLTDFLANYVGLKRSPIPFTAWKKTGSKLRAKCGINFAF 120

Query: 121 GGTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSM 180
           GGTG+FDT +PFPNMTTQINFFQ LIANSIFT  DIHSSIALVS SGNDYSFY ATNGS 
Sbjct: 121 GGTGVFDTLYPFPNMTTQINFFQNLIANSIFTSYDIHSSIALVSPSGNDYSFYLATNGSP 180

Query: 181 EGFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLV 240
           EG KPF++SVVNQI++NLKRI+ LGV+K+V++GLGP+GC P  TA S F KCN TM+SL 
Sbjct: 181 EGVKPFVISVVNQISVNLKRIYKLGVKKIVVVGLGPVGCYPSPTAPS-FKKCNETMNSLA 240

Query: 241 EFHNALLKQAVEKLNKETK--GSP-FFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCC 300
            FHN LLKQAVEKLN ETK  GSP FFIL++Y T+LSII+NKG+P K    F+TPLKPCC
Sbjct: 241 VFHNTLLKQAVEKLNSETKLGGSPNFFILNMYDTVLSIIKNKGNP-KVGATFQTPLKPCC 300

Query: 301 FGVSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351
           FGVSS FSCGSVD+ GNK YTLCK P LALFWD+VHPTQ+GWF  F SL S LK++
Sbjct: 301 FGVSSNFSCGSVDEHGNKMYTLCKRPDLALFWDTVHPTQKGWFATFNSLISTLKRI 351

BLAST of MC01g_new0307 vs. ExPASy TrEMBL
Match: A0A1S3CH67 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500887 PE=3 SV=1)

HSP 1 Score: 477 bits (1228), Expect = 1.69e-166
Identity = 248/354 (70.06%), Postives = 282/354 (79.66%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60
           M S KLLFS +  L +F L  GQGS+  H   FH  P +LFVFGDSYVDTGNI+VN SSA
Sbjct: 1   MASNKLLFSFLPFL-VFFLHFGQGSSAVH---FH--PLKLFVFGDSYVDTGNIHVNASSA 60

Query: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLK-SPVPFTTWQNVGVKRPRNGINFAFG 120
           RN PYGITFPGVPSGR SDGRVLTD+LA+Y GLK SP+PFT WQ  G K  + G+NF FG
Sbjct: 61  RNFPYGITFPGVPSGRFSDGRVLTDFLAKYVGLKKSPIPFTVWQRFGRKGAKYGMNFGFG 120

Query: 121 GTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSME 180
           GTG+FDTSFPFPNMTTQINFF+ LI NSIF+  DIHSSIALVS SGNDYSFY   NGS+E
Sbjct: 121 GTGVFDTSFPFPNMTTQINFFRNLIVNSIFSSYDIHSSIALVSPSGNDYSFYFTNNGSLE 180

Query: 181 GFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVE 240
           GFKPFI SVVNQI++NLKRI+ LGV+KVV++GLGP+GC P+ TA S F KCN TMDS+  
Sbjct: 181 GFKPFIFSVVNQISVNLKRIYKLGVKKVVVLGLGPVGCYPKPTAPS-FKKCNKTMDSIAV 240

Query: 241 FHNALLKQAVEKLNKETK-GSP-FFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFG 300
           FHN LLKQAVEKLN ETK GSP FFIL++Y T+LSII+NK +  K    F+TPLKPCCFG
Sbjct: 241 FHNTLLKQAVEKLNYETKEGSPNFFILNMYDTVLSIIKNKENR-KGGATFKTPLKPCCFG 300

Query: 301 VSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351
           V+S FSCGSVD++GNK YTLCK P LALFWD+VHPTQ GWF  F S  S LK +
Sbjct: 301 VNSNFSCGSVDERGNKMYTLCKRPDLALFWDAVHPTQNGWFATFSSFKSTLKHI 346

BLAST of MC01g_new0307 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 371.3 bits (952), Expect = 8.5e-103
Identity = 186/354 (52.54%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLG-----QGSARYHHRHFHLRPTRLFVFGDSYVDTGNINV 60
           MDS   LF  +F     SLL G     +GS + HH  +  RPT+LFVFGDSY DTGNI  
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHL-YPFRPTKLFVFGDSYADTGNIKK 60

Query: 61  NTSSARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGIN 120
             SS+   PYGITFPG P+GR SDGRV TD+LA++ G+KSP+P+      G KR + G+N
Sbjct: 61  AFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQYGMN 120

Query: 121 FAFGGTGIFDTSFPFPNMTTQINFFQKLI-ANSIFTLNDIHSSIALVSVSGNDYSFYQAT 180
           FA+GGTG+F+T  P PNMTTQI+ FQ ++    I+   ++ SS+ALVSV+GNDYS + A 
Sbjct: 121 FAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIAL 180

Query: 181 NGSMEGFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATM 240
           N     F  FI  VV+Q  +NL+RIH LGV+K+ +  L PLGCLP  T  +SF +CN T 
Sbjct: 181 NRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQ 240

Query: 241 DSLVEFHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPC 300
           ++LV  HN LL+Q V KLN ETK S F ILD+Y   L++ +NKGS      RFE+PLKPC
Sbjct: 241 NALVNLHNNLLQQVVAKLNNETKQSTFIILDLYNAFLTVFKNKGS-NPGSTRFESPLKPC 300

Query: 301 CFGVSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNL 349
           C GVS E++CGSVD+KG KKY +C +PK A FWD +HPT+EGW   +  L  +L
Sbjct: 301 CVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLRESL 352

BLAST of MC01g_new0307 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 355.5 bits (911), Expect = 4.8e-98
Identity = 180/354 (50.85%), Postives = 235/354 (66.38%), Query Frame = 0

Query: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHF-HLRPTRLFVFGDSYVDTGNINVNTSS 60
           M+  KLL SL F  S  S  LG  S    H +   LRP RLFVFGDSY DTGNI  + S 
Sbjct: 1   MELPKLLISL-FLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSD 60

Query: 61  ARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFG 120
           +  IPYGITFP  PSGR SDGRV TD+LARY G+KSP+P+T     G +R   G+N+A+G
Sbjct: 61  SWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYG 120

Query: 121 GTGIFDT-SFPFPNMTTQINFFQK-LIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGS 180
           GTG+F T   P PNMTTQI++FQ+ L A +I++ +D+ SS+ALVSV+GNDY+ + A    
Sbjct: 121 GTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRP 180

Query: 181 MEGFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSL 240
           +     F+  VV+QIA+N  RIH LGV K+V+  + PLGCLP +T  +SF +CNAT ++ 
Sbjct: 181 LTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNAS 240

Query: 241 VEFHNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFG 300
              HN LL +A+ +LN ETK S F +LD Y   L++ +NKG P     RF  PLKPCC G
Sbjct: 241 TNLHNYLLHKAIARLNNETKPSTFVVLDHYNAFLTVFKNKG-PEPGVSRFGNPLKPCCVG 300

Query: 301 VSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 352
           V+S + C +VD+KG KKY +C+DPK A FWD  HP++EGW   +  L  +LK +
Sbjct: 301 VNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLKAI 352

BLAST of MC01g_new0307 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 7.2e-70
Identity = 143/324 (44.14%), Postives = 195/324 (60.19%), Query Frame = 0

Query: 35  LRP---TRLFVFGDSYVDTGNINVNTSSARNIPYGITFPGVPSGRLSDGRVLTDYLARYF 94
           LRP    +LFVFGDSY DTGN   +T  A  IPYGITFPG PSGR  DG + TD+L +  
Sbjct: 7   LRPRKVPKLFVFGDSYADTGNTKRDT-EAWAIPYGITFPGKPSGRYCDGLIATDFLEKVL 66

Query: 95  GLKSPVPFTT-WQNVGVKRPRNGINFAFGGTGIFDTS--FPFPNMTTQINFFQKLIANSI 154
           G +SP  + T  ++ G+KR   G+NFAFGG+ + D+S   PFPN+T Q+NF   L+    
Sbjct: 67  GAESPYLYRTHGRDKGLKR---GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGR 126

Query: 155 FTLNDIHSSIALVSVSGNDYSFYQATNGSMEGFKPFIVSVVNQIAINLKRIHGLGVRKVV 214
              +   S ++L+S +G DY +Y   N    G K  +  VV+ + +N+  + GL  +K+ 
Sbjct: 127 VYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIA 186

Query: 215 MMGLGPLGCLPELTAASSFSKCNATMDSLVEFHNALLKQAVEKLNKET----KGSPFFIL 274
           +  L P+GCLP  T+ASSF  CN +  +LVE HN LLK+ V KLN+++    K   FFI+
Sbjct: 187 VTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFII 246

Query: 275 DIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSSEFSCGSVDKKGNKKYTLCKDPKLA 334
           DI+   +++++NKGS      RF+ P+K CC G      CG     G K YTLC DPK  
Sbjct: 247 DIHNAFMTVMKNKGS-----KRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDPKSF 306

Query: 335 LFWDSVHPTQEGWFVAFRSLGSNL 349
            FWD+VHPTQEGW   +  LG+ L
Sbjct: 307 FFWDAVHPTQEGWRSIYSVLGNPL 315

BLAST of MC01g_new0307 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 7.0e-65
Identity = 135/320 (42.19%), Postives = 182/320 (56.88%), Query Frame = 0

Query: 33  FHLRPTRLFVFGDSYVDTGNINVNTSSARNIPYGITFPGVPSGRLSDGRVLTDYLARYFG 92
           F  +PT+LFVFGDSY DTGN       +   P GITFPG+P+GR SDGRV TDYLA+Y G
Sbjct: 41  FGFKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIG 100

Query: 93  LKSPVPFTTWQNVGVKRPR----NGINFAFGGTGIFDTSFPF-PNMTTQINFFQKLIANS 152
           +++P+ +  W   G  RPR     G+NFA+GG G F+T F   P  + QI+ F++L+  +
Sbjct: 101 VRTPITY-KWGKYG--RPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRN 160

Query: 153 IFTLNDIHSSIALVSVSGNDYSFYQATNGSMEGFKPFIVSVVNQIAINLKRIHGLGVRKV 212
           +++  D++SS+A  S+ GNDY  Y   NGS EG       VV QI +++KRI  LGVRKV
Sbjct: 161 VYSPADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKV 220

Query: 213 VMMGLGPLGCLPELTAASSFSKCNATMDSLVEFHNALLKQAVEKLNKE---TKGSPFFIL 272
           ++    P  CLP+L        C+ T D+    HN+LL++ + KLN +        F  L
Sbjct: 221 LVALSPPQKCLPKLVTPKG---CD-TNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTL 280

Query: 273 DIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSSEFSCGSVDKKGNKKYTLCKDPKLA 332
           D+Y   ++I +NKG        F  P K CC      F CG     G K YTLC DPK  
Sbjct: 281 DLYNAFVTIFKNKG--VSGVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSF 340

Query: 333 LFWDSVHPTQEGWFVAFRSL 345
            FWD+VH + +GW   F  L
Sbjct: 341 FFWDNVHISDQGWRSVFSLL 350

BLAST of MC01g_new0307 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 9.2e-41
Identity = 104/299 (34.78%), Postives = 143/299 (47.83%), Query Frame = 0

Query: 57  TSSARNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINF 116
           T+ +  +PYGITFPG PSGR SDG   TD+L                             
Sbjct: 4   TALSWKLPYGITFPGKPSGRYSDGLTATDFL----------------------------- 63

Query: 117 AFGGTGIFDTSFP-FPNMTTQINFFQKL-IANSIFTLN-DIHSSIALVSVSGNDYSFYQA 176
              G+ +FD+     PN++TQ+ F   L +A  ++T++ D+ SS AL+S SG DY  +  
Sbjct: 64  ---GSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFID 123

Query: 177 TNGSMEGFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNAT 236
            N +M  +  F+  +V  I                                     CN +
Sbjct: 124 QNPNMAAYPAFVEFIVEDI------------------------------------HCNES 183

Query: 237 MDSLVEFHNALLKQAVEKLNKE----TKGSPFFILDIYGTLLSIIQNKGSPYKARPRFET 296
              LV  HN  LK+AV KLNKE    TKG  F I+D++   ++I++ KG+      RF++
Sbjct: 184 YSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILEKKGN-----KRFKS 224

Query: 297 PLKPCCFGVSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNL 349
           PLKPCC G      C  +D KG KKYTLC DPK A FWD ++PTQEGW   +  LG +L
Sbjct: 244 PLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLLGKSL 224