MC01g_new0306 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0306 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.1e-45
Identity = 115/315 (36.51%), Postives = 158/315 (50.16%), Query Frame = 0

Query: 37  KLYVFGDSYVDTGNLIGTDSES-KYPNGITWPGKPAGRFSDGHVLTDYFVKPMQIN---- 96
           KL+VFGDSY DTGN+    S S K+P GIT+PGKPAGRFSDG V TD+  K + I     
Sbjct: 43  KLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIP 102

Query: 97  --------------GVNFAVGGSGVFQTLSPVQNLTVQIDYFEELINNSTL-TPQDIDSS 156
                         G+NFA GG+GVF T +P+ N+T QID F+ ++    +  P ++ SS
Sbjct: 103 YFWKDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSS 162

Query: 157 IALVSVSGNDYVTYLIYN-----------------------------------------L 216
           +ALVSV+GNDY  ++  N                                          
Sbjct: 163 VALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGC 222

Query: 217 LTPDKLV----------ELVPEHHNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIH 272
           L P   V            +   HN+LLQ+ + KLN+E  T   +F I+DL+ AF +V  
Sbjct: 223 LPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNE--TKQSTFIILDLYNAFLTVFK 282

BLAST of MC01g_new0306 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 168.3 bits (425), Expect = 1.2e-40
Identity = 112/307 (36.48%), Postives = 153/307 (49.84%), Query Frame = 0

Query: 37  KLYVFGDSYVDTGNLIGTDSES-KYPNGITWPGKPAGRFSDGHVLTDYFVKPMQI----- 96
           KL+VFGDSY DTGN       S ++PNGIT+PG P GRFSDG V TDY  K + +     
Sbjct: 47  KLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTPIT 106

Query: 97  --------------NGVNFAVGGSGVFQTL-SPVQNLTVQIDYFEELINNSTLTPQDIDS 156
                          G+NFA GG+G F+T+   V   +VQID FE+L+  +  +P D++S
Sbjct: 107 YKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSPADLNS 166

Query: 157 SIALVSVSGNDYVTY----------------LIYNLLTPDK----------LVELVPEH- 216
           S+A  S+ GNDY+TY                ++  +L   K          LV L P   
Sbjct: 167 SVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALSPPQK 226

Query: 217 --------------------HNSLLQEGIKKLND-ETTTDDPSFSIIDLHAAFYSVIHNK 265
                               HNSLL++G+ KLND E   +D SF  +DL+ AF ++  NK
Sbjct: 227 CLPKLVTPKGCDTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYNAFVTIFKNK 286

BLAST of MC01g_new0306 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.6e-40
Identity = 111/311 (35.69%), Postives = 159/311 (51.13%), Query Frame = 0

Query: 36  NKLYVFGDSYVDTGNLIGTDSES-KYPNGITWPGKPAGRFSDGHVLTDYFVKPMQIN--- 95
           N+L+VFGDSY DTGN+  + S+S K P GIT+P KP+GRFSDG V TD+  + + I    
Sbjct: 38  NRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPI 97

Query: 96  ---------------GVNFAVGGSGVFQTL-SPVQNLTVQIDYFEELINNSTL-TPQDID 155
                          G+N+A GG+GVF+T  +P+ N+T QIDYF+ ++    + +P D+ 
Sbjct: 98  PYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLP 157

Query: 156 SSIALVSVSGNDYVTYLI-------------------------YNLLTPDKLV------- 215
           SS+ALVSV+GNDY T+L                           + L  +K+V       
Sbjct: 158 SSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPL 217

Query: 216 -------------------ELVPEHHNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSV 265
                                    HN LL + I +LN+ET     +F ++D + AF +V
Sbjct: 218 GCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNETKPS--TFVVLDHYNAFLTV 277

BLAST of MC01g_new0306 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 5.9e-32
Identity = 103/314 (32.80%), Postives = 142/314 (45.22%), Query Frame = 0

Query: 37  KLYVFGDSYVDTGNLIGTDSESKYPNGITWPGKPAGRFSDGHVLTDYFVKPM-------- 96
           KL+VFG+SY DTGN+  T    K P GIT+PGKP+GR+SDG   TD+  K +        
Sbjct: 34  KLFVFGNSYADTGNMKPTALSWKLPYGITFPGKPSGRYSDGLTATDFLAKQLGAKLPYLW 93

Query: 97  --------QIN-GVNFAVGGSGVFQTLSPVQ---NLTVQIDYFEE--LINNSTLTPQDID 156
                   ++N G+NFA GGS VF   SPV    N++ Q+ +     L         D+ 
Sbjct: 94  RTHGKKKVKLNRGMNFAFGGSEVFD--SPVDRSPNISTQVGFLVNLALARRVYTIDGDLA 153

Query: 157 SSIALVSVSGNDYVTYLIY--NLLTPDKLVELVPEH------------------------ 216
           SS AL+S SG DY  ++    N+      VE + E                         
Sbjct: 154 SSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPL 213

Query: 217 -------------------------HNSLLQEGIKKLN--DETTTDDPSFSIIDLHAAFY 272
                                    HN  L++ + KLN  D+  T    F I+DLH AF 
Sbjct: 214 GCLPRVTVASSFRSCNESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFM 273

BLAST of MC01g_new0306 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.9e-32
Identity = 95/310 (30.65%), Postives = 138/310 (44.52%), Query Frame = 0

Query: 33  RTFNKLYVFGDSYVDTGNLIGTDSESKYPNGITWPGKPAGRFSDGHVLTDYFVKPMQI-- 92
           R   KL+VFGDSY DTGN          P GIT+PGKP+GR+ DG + TD+  K +    
Sbjct: 10  RKVPKLFVFGDSYADTGNTKRDTEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAES 69

Query: 93  --------------NGVNFAVGGSGVFQTL--SPVQNLTVQIDYFEELINNSTLTPQDID 152
                          G+NFA GGS +  +   SP  N+T Q+++  +L+    +      
Sbjct: 70  PYLYRTHGRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDITP 129

Query: 153 SSIALVSVSGNDYVTYLIYN---------------------------LLTPDKLVELVP- 212
           S ++L+S +G DY+ Y+  N                           L     +  L P 
Sbjct: 130 SDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQPI 189

Query: 213 -----------------------EHHNSLLQEGIKKLNDETTT--DDPSFSIIDLHAAFY 269
                                  E HN LL++ + KLN+++     +  F IID+H AF 
Sbjct: 190 GCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFIIDIHNAFM 249

BLAST of MC01g_new0306 vs. NCBI nr
Match: XP_022133148.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 364 bits (934), Expect = 8.01e-123
Identity = 192/344 (55.81%), Postives = 225/344 (65.41%), Query Frame = 0

Query: 1   MESTKLFLPILFIVSFMAGQGLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSESKY 60
           MES KLFLP+LFI+S +AGQGLAH+YS+R++    NKL+VFGDSYVDTGN+I  D  SKY
Sbjct: 1   MESVKLFLPLLFIISLVAGQGLAHHYSLRRSFEAINKLFVFGDSYVDTGNVIDGDLVSKY 60

Query: 61  PNGITWPGKPAGRFSDGHVLTDYFVK----------------PMQINGVNFAVGGSGVFQ 120
           PNGITWPGKPAGR+SDG+VLTDYF                   M I+GVNFA GGSGVF 
Sbjct: 61  PNGITWPGKPAGRYSDGYVLTDYFASFLGLKFPIPYVQLHDVVMHIDGVNFAFGGSGVFT 120

Query: 121 TLSPVQNLTVQIDYFEELINNSTLTPQDIDSSIALVSVSGNDYVTYLIYNLLTPDKLV-- 180
           T+ PV  LT QID+F+  I+NSTLTPQ IDSSIALVSVSGNDY+TY + ++    +LV  
Sbjct: 121 TIFPVPKLTDQIDFFQGFIDNSTLTPQYIDSSIALVSVSGNDYMTYFVNHIFDWTRLVMG 180

Query: 181 ------------------------------------------------------ELVPEH 240
                                                                  LV ++
Sbjct: 181 LKPFIRSMVNTIRDDLKRIHSLGVKKVVVTTLGPLECVPMTTMFLEYERCVTILNLVVDY 240

Query: 241 HNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITFPKPLESCNKLVL 272
           HNSLLQE + KLN+ET TDDPSFSI+DLHAAF S+I NKGYP+ NITF  PLE CNKLV 
Sbjct: 241 HNSLLQEAVTKLNNETNTDDPSFSILDLHAAFSSIIQNKGYPKGNITFSTPLEPCNKLVT 300

BLAST of MC01g_new0306 vs. NCBI nr
Match: XP_022132562.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 288 bits (736), Expect = 9.19e-93
Identity = 170/351 (48.43%), Postives = 200/351 (56.98%), Query Frame = 0

Query: 1   MESTKLFLPILFIVSFMAGQGLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSESKY 60
           MESTKLF  + F VS M GQGL  +Y +RQ     NKL VFGDSYVDTGN     S   Y
Sbjct: 1   MESTKLFFLLSFFVSLMIGQGLGGHYPLRQHFEVSNKLLVFGDSYVDTGNEPSEQSFINY 60

Query: 61  PNGITWPGKPAGRFSDGHVLTDYFV------------------KPMQINGVNFAVGGSGV 120
           P G+T+PGKP GR+SDG+VLTDYF                   +P   +GVNF VGG+GV
Sbjct: 61  PYGMTFPGKPDGRYSDGYVLTDYFASFLGLESPIPYMQLYAVSRPELKDGVNFGVGGTGV 120

Query: 121 FQTLSPVQNLTVQIDYFEELINNSTLTPQDIDSSIALVSVSGNDYVTYLIYNLLT----- 180
           F+TL    N+T QID+ EELI+NSTLTP  IDSSIALVS+SGNDY+TYL+ N+L      
Sbjct: 121 FETLVSGPNITRQIDFLEELIDNSTLTPHYIDSSIALVSISGNDYMTYLVRNILDLVGFI 180

Query: 181 ----------PDKLVE-----------------------------------------LVP 240
                      D+ +E                                         LV 
Sbjct: 181 KGIKPFVRSMVDETIENLKRINSLGVKKVVVTALGPVECVPFMTFLISYEQCFPVLSLVA 240

Query: 241 EHHNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITFPKPLESCNKL 272
           ++HN LL++ + +LN E T D   FSI+DLH AF  +I NKGYPE NITFP PL+ C   
Sbjct: 241 DYHNILLRQAVDRLNREATNDS-HFSILDLHGAFSYIIQNKGYPEGNITFPTPLKPCCTG 300

BLAST of MC01g_new0306 vs. NCBI nr
Match: XP_022159398.1 (GDSL esterase/lipase At5g03610-like, partial [Momordica charantia])

HSP 1 Score: 234 bits (598), Expect = 4.61e-73
Identity = 137/263 (52.09%), Postives = 153/263 (58.17%), Query Frame = 0

Query: 21  GLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSESKYPNGITWPGKPAGRFSDGHVL 80
           GLAH+YS+R+ SR FNKL+VFGDSYVDTGN IGTD ES YPNGITW  KPAGR SDG VL
Sbjct: 3   GLAHHYSLRRTSRIFNKLFVFGDSYVDTGNFIGTDFESTYPNGITWSDKPAGRASDGRVL 62

Query: 81  TDYFVK----------------PMQINGVNFAVGGSGVFQTLSPVQNLTVQIDYFEELIN 140
           TDYF                   MQI+GVNFA GGSGVF+TL PV NL  QID+F+ LI+
Sbjct: 63  TDYFASFLGLESPIPYMQLHNVLMQIDGVNFAFGGSGVFKTLFPVPNLNDQIDFFQGLID 122

Query: 141 NSTLTPQDIDSSIALVSVSGNDYVTYLIYNLLTPDKLV---------------------- 200
           NS L PQ IDSSIALVSVSGNDY+TYL+ +      LV                      
Sbjct: 123 NSXLPPQYIDSSIALVSVSGNDYMTYLVNHFFDLAGLVQGLEPFIRSMVDNTIEDLKRIH 182

Query: 201 ---------------ELVP-------------------EHHNSLLQEGIKKLNDETTTDD 211
                          E VP                   ++HN LLQE I KLN ET TDD
Sbjct: 183 SLGVKKVVVTTLGPLECVPMLTLFLSYERCVPIVNSVADYHNYLLQEAIAKLNIETNTDD 242

BLAST of MC01g_new0306 vs. NCBI nr
Match: XP_023544735.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 222 bits (566), Expect = 3.80e-67
Identity = 144/347 (41.50%), Postives = 186/347 (53.60%), Query Frame = 0

Query: 1   MESTKLFLPILFIVSFMAGQGLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSE-SK 60
           MESTKLFLP  F +S ++G GLA ++  +      +KL+VFGDSYVDTGN   +D    K
Sbjct: 1   MESTKLFLPF-FTLSLLSGLGLARHHHHQHRHLQTSKLFVFGDSYVDTGNTSPSDPNYPK 60

Query: 61  YPNGITWPGKPAGRFSDGHVLTDYFVK--------PMQI----------NGVNFAVGGSG 120
            P G+T+PG P GRFS+G VLTDY  +        P ++          +GVNFA GG+G
Sbjct: 61  TPYGVTFPGHPDGRFSNGRVLTDYVARIIGLKSPIPYRVVEKEGVEGAKDGVNFAYGGTG 120

Query: 121 VFQTLSPVQNLTVQIDYFEELINNSTLTPQDIDSSIALVSVSGNDYVTYL---------- 180
           VF+T   +  +T QID+ + LI NST TPQDI+SS ALVSVSGNDY  YL          
Sbjct: 121 VFKTGYDLPTMTTQIDFLQYLIANSTFTPQDINSSFALVSVSGNDYSYYLSTNGSIKGFV 180

Query: 181 ----------------IYNL------LTPDKLVELVPE-------------------HHN 240
                           I++L      +T    ++ VPE                    HN
Sbjct: 181 PLIVRVVKQIGVNLKRIHSLGVKKISITALGPLQCVPEITSSSDFKHCNSSLALLVDFHN 240

Query: 241 SLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITFPKPLESCNK----- 268
            LL++ + KLN ET  +D  F I+DLHAAF S+I NKG P+ +I F  PL+ C       
Sbjct: 241 LLLRQAVDKLNKET--NDSPFFILDLHAAFLSIIQNKGDPQGSIKFETPLKPCCYGASPG 300

BLAST of MC01g_new0306 vs. NCBI nr
Match: XP_038883064.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 221 bits (564), Expect = 8.52e-67
Identity = 142/354 (40.11%), Postives = 185/354 (52.26%), Query Frame = 0

Query: 1   MESTKLFLPILFIVSFMAGQGLA-HYYSVRQASRTF--NKLYVFGDSYVDTGNLIGTDSE 60
           MES KLF+P  FI+S ++ +G A H+         F  +KL+VFGDSYVDTGN+  +DS 
Sbjct: 1   MESIKLFIPFFFILSLLSEKGFAGHHLHHHHLHHHFQPSKLFVFGDSYVDTGNISPSDSN 60

Query: 61  -SKYPNGITWPGKPAGRFSDGHVLTDYFVKPMQIN------------------GVNFAVG 120
             KYP GIT+PGKP GRFS+G VLTD+  + + +                   GVNFA G
Sbjct: 61  YPKYPYGITFPGKPDGRFSNGRVLTDFAARFIGLKSPIPYRHLEKIGIEGAKYGVNFAYG 120

Query: 121 GSGVFQTLSPVQNLTVQIDYFEELINNSTLTPQDIDSSIALVSVSGNDYVTYLIYN---- 180
           G+GVF+T   +  +T QID+ + LI +ST TP  I SS ALVSVSGNDY  YL  N    
Sbjct: 121 GTGVFKTGYDLPTMTTQIDFLQNLIADSTFTPNQISSSFALVSVSGNDYSYYLSTNGSIQ 180

Query: 181 --LLTPDKLV--------------------------ELVPE------------------- 240
             +   +K+V                          + VPE                   
Sbjct: 181 GFIPLIEKVVKQIRVNLKRIHSFGVKKISITALGPLQCVPEITVVSDFKECNSTISALVD 240

Query: 241 HHNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITFPKPLESCN--- 272
            HN LLQ+ + KLN ET  +D  F +++LH+AF S+I NKG P+ NITF  PL+ C    
Sbjct: 241 FHNFLLQQAVDKLNKET--NDSPFFVLNLHSAFSSIIQNKGVPQENITFATPLQPCCIGI 300

BLAST of MC01g_new0306 vs. ExPASy TrEMBL
Match: A0A6J1BY90 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111005821 PE=3 SV=1)

HSP 1 Score: 364 bits (934), Expect = 3.88e-123
Identity = 192/344 (55.81%), Postives = 225/344 (65.41%), Query Frame = 0

Query: 1   MESTKLFLPILFIVSFMAGQGLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSESKY 60
           MES KLFLP+LFI+S +AGQGLAH+YS+R++    NKL+VFGDSYVDTGN+I  D  SKY
Sbjct: 1   MESVKLFLPLLFIISLVAGQGLAHHYSLRRSFEAINKLFVFGDSYVDTGNVIDGDLVSKY 60

Query: 61  PNGITWPGKPAGRFSDGHVLTDYFVK----------------PMQINGVNFAVGGSGVFQ 120
           PNGITWPGKPAGR+SDG+VLTDYF                   M I+GVNFA GGSGVF 
Sbjct: 61  PNGITWPGKPAGRYSDGYVLTDYFASFLGLKFPIPYVQLHDVVMHIDGVNFAFGGSGVFT 120

Query: 121 TLSPVQNLTVQIDYFEELINNSTLTPQDIDSSIALVSVSGNDYVTYLIYNLLTPDKLV-- 180
           T+ PV  LT QID+F+  I+NSTLTPQ IDSSIALVSVSGNDY+TY + ++    +LV  
Sbjct: 121 TIFPVPKLTDQIDFFQGFIDNSTLTPQYIDSSIALVSVSGNDYMTYFVNHIFDWTRLVMG 180

Query: 181 ------------------------------------------------------ELVPEH 240
                                                                  LV ++
Sbjct: 181 LKPFIRSMVNTIRDDLKRIHSLGVKKVVVTTLGPLECVPMTTMFLEYERCVTILNLVVDY 240

Query: 241 HNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITFPKPLESCNKLVL 272
           HNSLLQE + KLN+ET TDDPSFSI+DLHAAF S+I NKGYP+ NITF  PLE CNKLV 
Sbjct: 241 HNSLLQEAVTKLNNETNTDDPSFSILDLHAAFSSIIQNKGYPKGNITFSTPLEPCNKLVT 300

BLAST of MC01g_new0306 vs. ExPASy TrEMBL
Match: A0A6J1BU66 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111005391 PE=3 SV=1)

HSP 1 Score: 288 bits (736), Expect = 4.45e-93
Identity = 170/351 (48.43%), Postives = 200/351 (56.98%), Query Frame = 0

Query: 1   MESTKLFLPILFIVSFMAGQGLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSESKY 60
           MESTKLF  + F VS M GQGL  +Y +RQ     NKL VFGDSYVDTGN     S   Y
Sbjct: 1   MESTKLFFLLSFFVSLMIGQGLGGHYPLRQHFEVSNKLLVFGDSYVDTGNEPSEQSFINY 60

Query: 61  PNGITWPGKPAGRFSDGHVLTDYFV------------------KPMQINGVNFAVGGSGV 120
           P G+T+PGKP GR+SDG+VLTDYF                   +P   +GVNF VGG+GV
Sbjct: 61  PYGMTFPGKPDGRYSDGYVLTDYFASFLGLESPIPYMQLYAVSRPELKDGVNFGVGGTGV 120

Query: 121 FQTLSPVQNLTVQIDYFEELINNSTLTPQDIDSSIALVSVSGNDYVTYLIYNLLT----- 180
           F+TL    N+T QID+ EELI+NSTLTP  IDSSIALVS+SGNDY+TYL+ N+L      
Sbjct: 121 FETLVSGPNITRQIDFLEELIDNSTLTPHYIDSSIALVSISGNDYMTYLVRNILDLVGFI 180

Query: 181 ----------PDKLVE-----------------------------------------LVP 240
                      D+ +E                                         LV 
Sbjct: 181 KGIKPFVRSMVDETIENLKRINSLGVKKVVVTALGPVECVPFMTFLISYEQCFPVLSLVA 240

Query: 241 EHHNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITFPKPLESCNKL 272
           ++HN LL++ + +LN E T D   FSI+DLH AF  +I NKGYPE NITFP PL+ C   
Sbjct: 241 DYHNILLRQAVDRLNREATNDS-HFSILDLHGAFSYIIQNKGYPEGNITFPTPLKPCCTG 300

BLAST of MC01g_new0306 vs. ExPASy TrEMBL
Match: A0A6J1DYP5 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111025813 PE=3 SV=1)

HSP 1 Score: 234 bits (598), Expect = 2.23e-73
Identity = 137/263 (52.09%), Postives = 153/263 (58.17%), Query Frame = 0

Query: 21  GLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSESKYPNGITWPGKPAGRFSDGHVL 80
           GLAH+YS+R+ SR FNKL+VFGDSYVDTGN IGTD ES YPNGITW  KPAGR SDG VL
Sbjct: 3   GLAHHYSLRRTSRIFNKLFVFGDSYVDTGNFIGTDFESTYPNGITWSDKPAGRASDGRVL 62

Query: 81  TDYFVK----------------PMQINGVNFAVGGSGVFQTLSPVQNLTVQIDYFEELIN 140
           TDYF                   MQI+GVNFA GGSGVF+TL PV NL  QID+F+ LI+
Sbjct: 63  TDYFASFLGLESPIPYMQLHNVLMQIDGVNFAFGGSGVFKTLFPVPNLNDQIDFFQGLID 122

Query: 141 NSTLTPQDIDSSIALVSVSGNDYVTYLIYNLLTPDKLV---------------------- 200
           NS L PQ IDSSIALVSVSGNDY+TYL+ +      LV                      
Sbjct: 123 NSXLPPQYIDSSIALVSVSGNDYMTYLVNHFFDLAGLVQGLEPFIRSMVDNTIEDLKRIH 182

Query: 201 ---------------ELVP-------------------EHHNSLLQEGIKKLNDETTTDD 211
                          E VP                   ++HN LLQE I KLN ET TDD
Sbjct: 183 SLGVKKVVVTTLGPLECVPMLTLFLSYERCVPIVNSVADYHNYLLQEAIAKLNIETNTDD 242

BLAST of MC01g_new0306 vs. ExPASy TrEMBL
Match: A0A5D3CAN3 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001200 PE=3 SV=1)

HSP 1 Score: 221 bits (562), Expect = 7.56e-67
Identity = 142/351 (40.46%), Postives = 183/351 (52.14%), Query Frame = 0

Query: 1   MESTKLFLPILFIVSFMAGQGLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSE-SK 60
           MESTKLFLP+LFI   ++G+G A ++   ++    +KL+VFGDSYVDTGN+  +DS   K
Sbjct: 1   MESTKLFLPLLFIFYLLSGKGFASHHHHHRSHFQPSKLFVFGDSYVDTGNVSPSDSNYPK 60

Query: 61  YPNGITWPGKPAGRFSDGHVLTDYFVKPMQI------------------NGVNFAVGGSG 120
           YP GIT+PGKPAGRFSDG VLTD+    + +                  +GVNFA GG+G
Sbjct: 61  YPYGITYPGKPAGRFSDGRVLTDFAANLIGLKSPIPYRHLEKVGLKGTKHGVNFAYGGTG 120

Query: 121 VFQTLSPVQNLTVQIDYFEELINNSTLTPQDIDSSIALVSVSGNDYVTYLIYN------L 180
           VF+T   +  +T QID+ + LI NST TP  I SS ALVSVSGNDY  YL  N      +
Sbjct: 121 VFKTGFDLPTMTTQIDFLQTLIANSTFTPDQIKSSFALVSVSGNDYSYYLSTNGPIQGFI 180

Query: 181 LTPDKLV--------------------------ELVPE-------------------HHN 240
              +K+V                          + VPE                    HN
Sbjct: 181 PLIEKVVKQISVNLRRIHSFGVKKISITALGPLQCVPEITALTDFKECNSTLSQLVDFHN 240

Query: 241 SLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITFPKPLESCN------ 272
            LL   + +LN ET  +D  F I++LH AF SVI NKG P+ NI F  PL+ C       
Sbjct: 241 FLLTRAVDELNKET--NDYPFFILNLHDAFSSVIQNKGNPQGNIKFENPLKPCCIGINPE 300

BLAST of MC01g_new0306 vs. ExPASy TrEMBL
Match: A0A1S3CH96 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500885 PE=3 SV=1)

HSP 1 Score: 221 bits (562), Expect = 7.56e-67
Identity = 142/351 (40.46%), Postives = 183/351 (52.14%), Query Frame = 0

Query: 1   MESTKLFLPILFIVSFMAGQGLAHYYSVRQASRTFNKLYVFGDSYVDTGNLIGTDSE-SK 60
           MESTKLFLP+LFI   ++G+G A ++   ++    +KL+VFGDSYVDTGN+  +DS   K
Sbjct: 1   MESTKLFLPLLFIFYLLSGKGFASHHHHHRSHFQPSKLFVFGDSYVDTGNVSPSDSNYPK 60

Query: 61  YPNGITWPGKPAGRFSDGHVLTDYFVKPMQI------------------NGVNFAVGGSG 120
           YP GIT+PGKPAGRFSDG VLTD+    + +                  +GVNFA GG+G
Sbjct: 61  YPYGITYPGKPAGRFSDGRVLTDFAANLIGLKSPIPYRHLEKVGLKGTKHGVNFAYGGTG 120

Query: 121 VFQTLSPVQNLTVQIDYFEELINNSTLTPQDIDSSIALVSVSGNDYVTYLIYN------L 180
           VF+T   +  +T QID+ + LI NST TP  I SS ALVSVSGNDY  YL  N      +
Sbjct: 121 VFKTGFDLPTMTTQIDFLQTLIANSTFTPDQIKSSFALVSVSGNDYSYYLSTNGPIQGFI 180

Query: 181 LTPDKLV--------------------------ELVPE-------------------HHN 240
              +K+V                          + VPE                    HN
Sbjct: 181 PLIEKVVKQISVNLRRIHSFGVKKISITALGPLQCVPEITALTDFKECNSTLSQLVDFHN 240

Query: 241 SLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITFPKPLESCN------ 272
            LL   + +LN ET  +D  F I++LH AF SVI NKG P+ NI F  PL+ C       
Sbjct: 241 FLLTRAVDELNKET--NDYPFFILNLHDAFSSVIQNKGNPQGNIKFENPLKPCCIGINPE 300

BLAST of MC01g_new0306 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 182.6 bits (462), Expect = 4.3e-46
Identity = 115/315 (36.51%), Postives = 158/315 (50.16%), Query Frame = 0

Query: 37  KLYVFGDSYVDTGNLIGTDSES-KYPNGITWPGKPAGRFSDGHVLTDYFVKPMQIN---- 96
           KL+VFGDSY DTGN+    S S K+P GIT+PGKPAGRFSDG V TD+  K + I     
Sbjct: 43  KLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIP 102

Query: 97  --------------GVNFAVGGSGVFQTLSPVQNLTVQIDYFEELINNSTL-TPQDIDSS 156
                         G+NFA GG+GVF T +P+ N+T QID F+ ++    +  P ++ SS
Sbjct: 103 YFWKDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSS 162

Query: 157 IALVSVSGNDYVTYLIYN-----------------------------------------L 216
           +ALVSV+GNDY  ++  N                                          
Sbjct: 163 VALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGC 222

Query: 217 LTPDKLV----------ELVPEHHNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSVIH 272
           L P   V            +   HN+LLQ+ + KLN+E  T   +F I+DL+ AF +V  
Sbjct: 223 LPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNE--TKQSTFIILDLYNAFLTVFK 282

BLAST of MC01g_new0306 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 8.4e-42
Identity = 112/307 (36.48%), Postives = 153/307 (49.84%), Query Frame = 0

Query: 37  KLYVFGDSYVDTGNLIGTDSES-KYPNGITWPGKPAGRFSDGHVLTDYFVKPMQI----- 96
           KL+VFGDSY DTGN       S ++PNGIT+PG P GRFSDG V TDY  K + +     
Sbjct: 47  KLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTPIT 106

Query: 97  --------------NGVNFAVGGSGVFQTL-SPVQNLTVQIDYFEELINNSTLTPQDIDS 156
                          G+NFA GG+G F+T+   V   +VQID FE+L+  +  +P D++S
Sbjct: 107 YKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSPADLNS 166

Query: 157 SIALVSVSGNDYVTY----------------LIYNLLTPDK----------LVELVPEH- 216
           S+A  S+ GNDY+TY                ++  +L   K          LV L P   
Sbjct: 167 SVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALSPPQK 226

Query: 217 --------------------HNSLLQEGIKKLND-ETTTDDPSFSIIDLHAAFYSVIHNK 265
                               HNSLL++G+ KLND E   +D SF  +DL+ AF ++  NK
Sbjct: 227 CLPKLVTPKGCDTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYNAFVTIFKNK 286

BLAST of MC01g_new0306 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 1.1e-41
Identity = 111/311 (35.69%), Postives = 159/311 (51.13%), Query Frame = 0

Query: 36  NKLYVFGDSYVDTGNLIGTDSES-KYPNGITWPGKPAGRFSDGHVLTDYFVKPMQIN--- 95
           N+L+VFGDSY DTGN+  + S+S K P GIT+P KP+GRFSDG V TD+  + + I    
Sbjct: 38  NRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPI 97

Query: 96  ---------------GVNFAVGGSGVFQTL-SPVQNLTVQIDYFEELINNSTL-TPQDID 155
                          G+N+A GG+GVF+T  +P+ N+T QIDYF+ ++    + +P D+ 
Sbjct: 98  PYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLP 157

Query: 156 SSIALVSVSGNDYVTYLI-------------------------YNLLTPDKLV------- 215
           SS+ALVSV+GNDY T+L                           + L  +K+V       
Sbjct: 158 SSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPL 217

Query: 216 -------------------ELVPEHHNSLLQEGIKKLNDETTTDDPSFSIIDLHAAFYSV 265
                                    HN LL + I +LN+ET     +F ++D + AF +V
Sbjct: 218 GCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNETKPS--TFVVLDHYNAFLTV 277

BLAST of MC01g_new0306 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 139.4 bits (350), Expect = 4.2e-33
Identity = 95/310 (30.65%), Postives = 138/310 (44.52%), Query Frame = 0

Query: 33  RTFNKLYVFGDSYVDTGNLIGTDSESKYPNGITWPGKPAGRFSDGHVLTDYFVKPMQI-- 92
           R   KL+VFGDSY DTGN          P GIT+PGKP+GR+ DG + TD+  K +    
Sbjct: 10  RKVPKLFVFGDSYADTGNTKRDTEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAES 69

Query: 93  --------------NGVNFAVGGSGVFQTL--SPVQNLTVQIDYFEELINNSTLTPQDID 152
                          G+NFA GGS +  +   SP  N+T Q+++  +L+    +      
Sbjct: 70  PYLYRTHGRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDITP 129

Query: 153 SSIALVSVSGNDYVTYLIYN---------------------------LLTPDKLVELVP- 212
           S ++L+S +G DY+ Y+  N                           L     +  L P 
Sbjct: 130 SDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQPI 189

Query: 213 -----------------------EHHNSLLQEGIKKLNDETTT--DDPSFSIIDLHAAFY 269
                                  E HN LL++ + KLN+++     +  F IID+H AF 
Sbjct: 190 GCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFIIDIHNAFM 249

BLAST of MC01g_new0306 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 7.7e-27
Identity = 84/239 (35.15%), Postives = 116/239 (48.54%), Query Frame = 0

Query: 59  KYPNGITWPGKPAGRFSDGHVLTDYFVKPMQINGVNFAVGGSGVFQTLSPVQ---NLTVQ 118
           K P GIT+PGKP+GR+SDG   TD+               GS VF   SPV    N++ Q
Sbjct: 9   KLPYGITFPGKPSGRYSDGLTATDFL--------------GSEVFD--SPVDRSPNISTQ 68

Query: 119 IDYFEE--LINNSTLTPQDIDSSIALVSVSGNDYVTYLIY--NLLTPDKLVELVPEH--- 178
           + +     L         D+ SS AL+S SG DY  ++    N+      VE + E    
Sbjct: 69  VGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIHC 128

Query: 179 ----------HNSLLQEGIKKLN--DETTTDDPSFSIIDLHAAFYSVIHNKGYPERNITF 238
                     HN  L++ + KLN  D+  T    F I+DLH AF +++  KG    N  F
Sbjct: 129 NESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILEKKG----NKRF 188

Query: 239 PKPLESCNK----LVLEQGTPKYELCEHPKSAFYWDFAHPTDEGWRAAFSYLTDTLHKS 272
             PL+ C +     +  +G  KY LC  PKSAF+WD  +PT EGWR+ +S L  +L +S
Sbjct: 189 KSPLKPCCEGDCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLLGKSLTES 227