MC01g_new0304 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0304
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	GDSL esterase/lipase At5g03610-like
Location	MC01: 12511860 .. 12512423 (+)
RNA-Seq Expression	MC01g_new0304
Synteny	MC01g_new0304

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTCAACAAAGTTGTTCTTCCCCCTCTTGTTCATCTCCCTCATGGCAGGTGTGTGCGATATGATTTTTGTATTGGTTTAGAATACTACTTTACATTTACATGTGTTTGTTTTCCAGGACAAGGACTAGGCGGTCATTATTCTATCCACAAGCATTTTGAGGCTACCAATAAGTTGTTTGTGTTTGGGGATTCCTACGTTGACACCAGCAATGAAATATCCAACGATAGTTTCATTAAGTATCCATATGGAATTATCTTTCTCGGCAAACCTGATGGACGTAACTCTGATGGTTATGTTCTCACAGATTACTTTGGTAAATCTAAATGATTTTTAACAATTTAACTATTTTAATAGTTGAATGTATTTTTATTAGTAGATTAATACATATAATACATCTTTGTCTACACCTGCAGCGAGTTTTCTAAACCTGGAGTCTTCGATCCCATACATGCAATTGTATGATGTAGTAAATCCAGAATTATTAGATGGTGTCAACTTTGCTTTTGGTGGAACTGGTGTGTTTGATACACAAATACACAAATTTCTGCGCCAAACTTGA

mRNA sequence

ATGGAGTCAACAAAGTTGTTCTTCCCCCTCTTGTTCATCTCCCTCATGGCAGGACAAGGACTAGGCGGTCATTATTCTATCCACAAGCATTTTGAGGCTACCAATAAGTTGTTTGTGTTTGGGGATTCCTACGTTGACACCAGCAATGAAATATCCAACGATAGTTTCATTAAGTATCCATATGGAATTATCTTTCTCGGCAAACCTGATGGACGTAACTCTGATGGTTATGTTCTCACAGATTACTTTGCGAGTTTTCTAAACCTGGAGTCTTCGATCCCATACATGCAATTGTATGATGTAGTAAATCCAGAATTATTAGATGGTGTCAACTTTGCTTTTGGTGGAACTGGTGTGTTTGATACACAAATACACAAATTTCTGCGCCAAACTTGA

Coding sequence (CDS)

ATGGAGTCAACAAAGTTGTTCTTCCCCCTCTTGTTCATCTCCCTCATGGCAGGACAAGGACTAGGCGGTCATTATTCTATCCACAAGCATTTTGAGGCTACCAATAAGTTGTTTGTGTTTGGGGATTCCTACGTTGACACCAGCAATGAAATATCCAACGATAGTTTCATTAAGTATCCATATGGAATTATCTTTCTCGGCAAACCTGATGGACGTAACTCTGATGGTTATGTTCTCACAGATTACTTTGCGAGTTTTCTAAACCTGGAGTCTTCGATCCCATACATGCAATTGTATGATGTAGTAAATCCAGAATTATTAGATGGTGTCAACTTTGCTTTTGGTGGAACTGGTGTGTTTGATACACAAATACACAAATTTCTGCGCCAAACTTGA

Protein sequence

MESTKLFFPLLFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGVFDTQIHKFLRQT

Homology

[Close]

BLAST of MC01g_new0304 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 9.5e-20
Identity = 61/126 (48.41%), Postives = 77/126 (61.11%), Query Frame = 0


Query: 5   KLFFPL---LFISLMAGQ--GL-GGHYSIHKHFEATNKLFVFGDSYVDTSN-EISNDSFI 64
           KLFF L   L  SL+ G+  G+ G + + H +     KLFVFGDSY DT N + +  S  
Sbjct: 6   KLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIKKAFSSSW 65

Query: 65  KYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGT 124
           K+PYGI F GKP GR SDG V TD+ A F+ ++S IPY          L  G+NFA+GGT
Sbjct: 66  KFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQYGMNFAYGGT 125

[Close]

BLAST of MC01g_new0304 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 4.0e-18
Identity = 57/128 (44.53%), Postives = 72/128 (56.25%), Query Frame = 0


Query: 1   MESTKLFFPLLFISLMA-GQGLGGHYSIHKHFE--ATNKLFVFGDSYVDTSN--EISNDS 60
           ME  KL   L   S  +   G    Y  H + +    N+LFVFGDSY DT N  +  +DS
Sbjct: 1   MELPKLLISLFLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDS 60

Query: 61  FIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFG 120
           + K PYGI F  KP GR SDG V TD+ A +L ++S IPY          LL G+N+A+G
Sbjct: 61  W-KIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYG 120

Query: 121 GTGVFDTQ 124
           GTGVF T+
Sbjct: 121 GTGVFKTK 127

[Close]

BLAST of MC01g_new0304 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 4.1e-15
Identity = 49/119 (41.18%), Postives = 66/119 (55.46%), Query Frame = 0


Query: 11  LFISLMAGQGLGGHYSIHKHFEATN--KLFVFGDSYVDTSNEISNDSFIKYPYGIIFLGK 70
           LF SL    G+ G    ++  +A    KLFVFG+SY DT N        K PYGI F GK
Sbjct: 7   LFFSLSLFFGINGAVGSNQPSQANKNPKLFVFGNSYADTGNMKPTALSWKLPYGITFPGK 66

Query: 71  PDGRNSDGYVLTDYFASFLNLESSIPYM-QLYDVVNPELLDGVNFAFGGTGVFDTQIHK 127
           P GR SDG   TD+ A    L + +PY+ + +     +L  G+NFAFGG+ VFD+ + +
Sbjct: 67  PSGRYSDGLTATDFLAK--QLGAKLPYLWRTHGKKKVKLNRGMNFAFGGSEVFDSPVDR 123

[Close]

BLAST of MC01g_new0304 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 3.9e-13
Identity = 40/87 (45.98%), Postives = 49/87 (56.32%), Query Frame = 0


Query: 36  KLFVFGDSYVDTSNEISNDSFIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPY 95
           KLFVFGDSY DT N   +      PYGI F GKP GR  DG + TD+    L  ES  PY
Sbjct: 14  KLFVFGDSYADTGNTKRDTEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAES--PY 73

Query: 96  MQLYDVVNPELLDGVNFAFGGTGVFDT 123
           +      +  L  G+NFAFGG+ + D+
Sbjct: 74  LYRTHGRDKGLKRGMNFAFGGSKMLDS 98

[Close]

BLAST of MC01g_new0304 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 9.5e-12
Identity = 41/90 (45.56%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 36  KLFVFGDSYVDTSNE--ISNDSFIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSI 95
           KLFVFGDSY DT N   +   S+ ++P GI F G P GR SDG V TDY A ++ + + I
Sbjct: 47  KLFVFGDSYADTGNTPFLIVPSW-RFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTPI 106

Query: 96  PY-MQLYDVVNPELLDGVNFAFGGTGVFDT 123
            Y    Y      +  G+NFA+GG G F+T
Sbjct: 107 TYKWGKYGRPRLAVKRGMNFAYGGAGAFET 135

[Close]

BLAST of MC01g_new0304 vs. NCBI nr
Match: XP_022133147.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 275 bits (702), Expect = 3.61e-93
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MESTKLFFPLLFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKYP 60
           MESTKLFFPLLFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKYP
Sbjct: 1   MESTKLFFPLLFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKYP 60

Query: 61  YGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGVF 120
           YGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGVF
Sbjct: 61  YGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGVF 120

Query: 121 DTQIHKFLRQT 131
           DTQIHKFLRQT
Sbjct: 121 DTQIHKFLRQT 131

[Close]

BLAST of MC01g_new0304 vs. NCBI nr
Match: XP_022132562.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 191 bits (484), Expect = 4.55e-57
Identity = 93/125 (74.40%), Postives = 100/125 (80.00%), Query Frame = 0


Query: 1   MESTKLFFPL-LFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKY 60
           MESTKLFF L  F+SLM GQGLGGHY + +HFE +NKL VFGDSYVDT NE S  SFI Y
Sbjct: 1   MESTKLFFLLSFFVSLMIGQGLGGHYPLRQHFEVSNKLLVFGDSYVDTGNEPSEQSFINY 60

Query: 61  PYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGV 120
           PYG+ F GKPDGR SDGYVLTDYFASFL LES IPYMQLY V  PEL DGVNF  GGTGV
Sbjct: 61  PYGMTFPGKPDGRYSDGYVLTDYFASFLGLESPIPYMQLYAVSRPELKDGVNFGVGGTGV 120

Query: 121 FDTQI 124
           F+T +
Sbjct: 121 FETLV 125

[Close]

BLAST of MC01g_new0304 vs. NCBI nr
Match: XP_022133148.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 165 bits (418), Expect = 3.36e-47
Identity = 86/123 (69.92%), Postives = 95/123 (77.24%), Query Frame = 0


Query: 1   MESTKLFFPLLFI-SLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKY 60
           MES KLF PLLFI SL+AGQGL  HYS+ + FEA NKLFVFGDSYVDT N I  D   KY
Sbjct: 1   MESVKLFLPLLFIISLVAGQGLAHHYSLRRSFEAINKLFVFGDSYVDTGNVIDGDLVSKY 60

Query: 61  PYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGV 120
           P GI + GKP GR SDGYVLTDYFASFL L+  IPY+QL+DVV    +DGVNFAFGG+GV
Sbjct: 61  PNGITWPGKPAGRYSDGYVLTDYFASFLGLKFPIPYVQLHDVVMH--IDGVNFAFGGSGV 120

Query: 121 FDT 122
           F T
Sbjct: 121 FTT 121

[Close]

BLAST of MC01g_new0304 vs. NCBI nr
Match: XP_004143312.1 (GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48249.1 hypothetical protein Csa_003600 [Cucumis sativus])

HSP 1 Score: 131 bits (329), Expect = 6.48e-34
Identity = 73/128 (57.03%), Postives = 88/128 (68.75%), Query Frame = 0


Query: 1   MESTKLFFPLLFI-SLMAGQGLGGHYS----IHKHFEATNKLFVFGDSYVDTSNEISNDS 60
           MESTKLF PLLFI SL++G+G GGH+     +H HF+ T KLFVFGDSYVDT N   +DS
Sbjct: 1   MESTKLFLPLLFILSLLSGKGFGGHHHHHHHVHSHFKPT-KLFVFGDSYVDTGNVSPSDS 60

Query: 61  -FIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAF 120
            +  YPYGI + GKP GR SDG VL+D+ A+ +  +S IPY  L  V       GVNFA+
Sbjct: 61  NYPTYPYGITYPGKPAGRFSDGRVLSDFAANLIGQKSPIPYRHLEKVGIKGTKYGVNFAY 120

Query: 121 GGTGVFDT 122
           GGTGVF T
Sbjct: 121 GGTGVFKT 127

[Close]

BLAST of MC01g_new0304 vs. NCBI nr
Match: XP_008462554.1 (PREDICTED: GDSL esterase/lipase At5g03610-like [Cucumis melo] >KAA0025272.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07396.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 130 bits (327), Expect = 1.21e-33
Identity = 70/124 (56.45%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 1   MESTKLFFPLLFIS-LMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDS-FIK 60
           MESTKLF PLLFI  L++G+G   H+  H+     +KLFVFGDSYVDT N   +DS + K
Sbjct: 1   MESTKLFLPLLFIFYLLSGKGFASHHHHHRSHFQPSKLFVFGDSYVDTGNVSPSDSNYPK 60

Query: 61  YPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTG 120
           YPYGI + GKP GR SDG VLTD+ A+ + L+S IPY  L  V       GVNFA+GGTG
Sbjct: 61  YPYGITYPGKPAGRFSDGRVLTDFAANLIGLKSPIPYRHLEKVGLKGTKHGVNFAYGGTG 120

Query: 121 VFDT 122
           VF T
Sbjct: 121 VFKT 124

[Close]

BLAST of MC01g_new0304 vs. ExPASy TrEMBL
Match: A0A6J1BVU4 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111005820 PE=4 SV=1)

HSP 1 Score: 275 bits (702), Expect = 1.75e-93
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MESTKLFFPLLFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKYP 60
           MESTKLFFPLLFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKYP
Sbjct: 1   MESTKLFFPLLFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKYP 60

Query: 61  YGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGVF 120
           YGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGVF
Sbjct: 61  YGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGVF 120

Query: 121 DTQIHKFLRQT 131
           DTQIHKFLRQT
Sbjct: 121 DTQIHKFLRQT 131

[Close]

BLAST of MC01g_new0304 vs. ExPASy TrEMBL
Match: A0A6J1BU66 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111005391 PE=3 SV=1)

HSP 1 Score: 191 bits (484), Expect = 2.20e-57
Identity = 93/125 (74.40%), Postives = 100/125 (80.00%), Query Frame = 0


Query: 1   MESTKLFFPL-LFISLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKY 60
           MESTKLFF L  F+SLM GQGLGGHY + +HFE +NKL VFGDSYVDT NE S  SFI Y
Sbjct: 1   MESTKLFFLLSFFVSLMIGQGLGGHYPLRQHFEVSNKLLVFGDSYVDTGNEPSEQSFINY 60

Query: 61  PYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGV 120
           PYG+ F GKPDGR SDGYVLTDYFASFL LES IPYMQLY V  PEL DGVNF  GGTGV
Sbjct: 61  PYGMTFPGKPDGRYSDGYVLTDYFASFLGLESPIPYMQLYAVSRPELKDGVNFGVGGTGV 120

Query: 121 FDTQI 124
           F+T +
Sbjct: 121 FETLV 125

[Close]

BLAST of MC01g_new0304 vs. ExPASy TrEMBL
Match: A0A6J1BY90 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111005821 PE=3 SV=1)

HSP 1 Score: 165 bits (418), Expect = 1.63e-47
Identity = 86/123 (69.92%), Postives = 95/123 (77.24%), Query Frame = 0


Query: 1   MESTKLFFPLLFI-SLMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIKY 60
           MES KLF PLLFI SL+AGQGL  HYS+ + FEA NKLFVFGDSYVDT N I  D   KY
Sbjct: 1   MESVKLFLPLLFIISLVAGQGLAHHYSLRRSFEAINKLFVFGDSYVDTGNVIDGDLVSKY 60

Query: 61  PYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGV 120
           P GI + GKP GR SDGYVLTDYFASFL L+  IPY+QL+DVV    +DGVNFAFGG+GV
Sbjct: 61  PNGITWPGKPAGRYSDGYVLTDYFASFLGLKFPIPYVQLHDVVMH--IDGVNFAFGGSGV 120

Query: 121 FDT 122
           F T
Sbjct: 121 FTT 121

[Close]

BLAST of MC01g_new0304 vs. ExPASy TrEMBL
Match: A0A0A0KHF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452010 PE=3 SV=1)

HSP 1 Score: 131 bits (329), Expect = 3.14e-34
Identity = 73/128 (57.03%), Postives = 88/128 (68.75%), Query Frame = 0


Query: 1   MESTKLFFPLLFI-SLMAGQGLGGHYS----IHKHFEATNKLFVFGDSYVDTSNEISNDS 60
           MESTKLF PLLFI SL++G+G GGH+     +H HF+ T KLFVFGDSYVDT N   +DS
Sbjct: 1   MESTKLFLPLLFILSLLSGKGFGGHHHHHHHVHSHFKPT-KLFVFGDSYVDTGNVSPSDS 60

Query: 61  -FIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAF 120
            +  YPYGI + GKP GR SDG VL+D+ A+ +  +S IPY  L  V       GVNFA+
Sbjct: 61  NYPTYPYGITYPGKPAGRFSDGRVLSDFAANLIGQKSPIPYRHLEKVGIKGTKYGVNFAY 120

Query: 121 GGTGVFDT 122
           GGTGVF T
Sbjct: 121 GGTGVFKT 127

[Close]

BLAST of MC01g_new0304 vs. ExPASy TrEMBL
Match: A0A5D3CAN3 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001200 PE=3 SV=1)

HSP 1 Score: 130 bits (327), Expect = 5.84e-34
Identity = 70/124 (56.45%), Postives = 84/124 (67.74%), Query Frame = 0


Query: 1   MESTKLFFPLLFIS-LMAGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDS-FIK 60
           MESTKLF PLLFI  L++G+G   H+  H+     +KLFVFGDSYVDT N   +DS + K
Sbjct: 1   MESTKLFLPLLFIFYLLSGKGFASHHHHHRSHFQPSKLFVFGDSYVDTGNVSPSDSNYPK 60

Query: 61  YPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTG 120
           YPYGI + GKP GR SDG VLTD+ A+ + L+S IPY  L  V       GVNFA+GGTG
Sbjct: 61  YPYGITYPGKPAGRFSDGRVLTDFAANLIGLKSPIPYRHLEKVGLKGTKHGVNFAYGGTG 120

Query: 121 VFDT 122
           VF T
Sbjct: 121 VFKT 124

[Close]

BLAST of MC01g_new0304 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 97.8 bits (242), Expect = 6.8e-21
Identity = 61/126 (48.41%), Postives = 77/126 (61.11%), Query Frame = 0


Query: 5   KLFFPL---LFISLMAGQ--GL-GGHYSIHKHFEATNKLFVFGDSYVDTSN-EISNDSFI 64
           KLFF L   L  SL+ G+  G+ G + + H +     KLFVFGDSY DT N + +  S  
Sbjct: 6   KLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIKKAFSSSW 65

Query: 65  KYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGT 124
           K+PYGI F GKP GR SDG V TD+ A F+ ++S IPY          L  G+NFA+GGT
Sbjct: 66  KFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQYGMNFAYGGT 125

[Close]

BLAST of MC01g_new0304 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 92.4 bits (228), Expect = 2.8e-19
Identity = 57/128 (44.53%), Postives = 72/128 (56.25%), Query Frame = 0


Query: 1   MESTKLFFPLLFISLMA-GQGLGGHYSIHKHFE--ATNKLFVFGDSYVDTSN--EISNDS 60
           ME  KL   L   S  +   G    Y  H + +    N+LFVFGDSY DT N  +  +DS
Sbjct: 1   MELPKLLISLFLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDS 60

Query: 61  FIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFG 120
           + K PYGI F  KP GR SDG V TD+ A +L ++S IPY          LL G+N+A+G
Sbjct: 61  W-KIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYG 120

Query: 121 GTGVFDTQ 124
           GTGVF T+
Sbjct: 121 GTGVFKTK 127

[Close]

BLAST of MC01g_new0304 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 2.7e-14
Identity = 40/87 (45.98%), Postives = 49/87 (56.32%), Query Frame = 0


Query: 36  KLFVFGDSYVDTSNEISNDSFIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSIPY 95
           KLFVFGDSY DT N   +      PYGI F GKP GR  DG + TD+    L  ES  PY
Sbjct: 14  KLFVFGDSYADTGNTKRDTEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAES--PY 73

Query: 96  MQLYDVVNPELLDGVNFAFGGTGVFDT 123
           +      +  L  G+NFAFGG+ + D+
Sbjct: 74  LYRTHGRDKGLKRGMNFAFGGSKMLDS 98

[Close]

BLAST of MC01g_new0304 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 6.8e-13
Identity = 41/90 (45.56%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 36  KLFVFGDSYVDTSNE--ISNDSFIKYPYGIIFLGKPDGRNSDGYVLTDYFASFLNLESSI 95
           KLFVFGDSY DT N   +   S+ ++P GI F G P GR SDG V TDY A ++ + + I
Sbjct: 47  KLFVFGDSYADTGNTPFLIVPSW-RFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTPI 106

Query: 96  PY-MQLYDVVNPELLDGVNFAFGGTGVFDT 123
            Y    Y      +  G+NFA+GG G F+T
Sbjct: 107 TYKWGKYGRPRLAVKRGMNFAYGGAGAFET 135

[Close]

BLAST of MC01g_new0304 vs. TAIR 10
Match: AT1G20132.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 7.0e-10
Identity = 39/108 (36.11%), Postives = 59/108 (54.63%), Query Frame = 0


Query: 17  AGQGLGGHYSIHKHFEATNKLFVFGDSYVDTSNEISNDSFIK---YPYGIIF-LGKPDGR 76
           +G G     S   H + T  +F FGDS +DT N  +  + +K    PYG  F LG   GR
Sbjct: 42  SGSGPSPGPSPSPHNKTTPAVFFFGDSIIDTGNNNNLTTEMKCNFSPYGKDFPLGVATGR 101

Query: 77  NSDGYVLTDYFASFLNLESSIPYMQLYDVVNPELLDGVNFAFGGTGVF 121
            S+G V++DY + +L ++  +P     +V   +LL GV+FA GG+G +
Sbjct: 102 FSNGKVVSDYISEYLGVKPIVPAYFDPNVQLEDLLTGVSFASGGSGYY 149

The following BLAST results are available for this feature:

BLAST of MC01g_new0304 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZS7	9.5e-20	48.41	GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2...	[more]
Q9SF94	4.0e-18	44.53	GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2...	[more]
Q9LZS9	4.1e-15	41.18	GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3...	[more]
Q9LZS8	3.9e-13	45.98	GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3...	[more]
Q8RWJ4	9.5e-12	45.56	GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2...	[more]

BLAST of MC01g_new0304 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133147.1	3.61e-93	100.00	GDSL esterase/lipase At5g03610-like [Momordica charantia]	[more]
XP_022132562.1	4.55e-57	74.40	GDSL esterase/lipase At5g03610-like [Momordica charantia]	[more]
XP_022133148.1	3.36e-47	69.92	GDSL esterase/lipase At5g03610-like [Momordica charantia]	[more]
XP_004143312.1	6.48e-34	57.03	GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48249.1 hypothetical protei...	[more]
XP_008462554.1	1.21e-33	56.45	PREDICTED: GDSL esterase/lipase At5g03610-like [Cucumis melo] >KAA0025272.1 GDSL...	[more]

BLAST of MC01g_new0304 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVU4	1.75e-93	100.00	GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC1110058...	[more]
A0A6J1BU66	2.20e-57	74.40	GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC1110053...	[more]
A0A6J1BY90	1.63e-47	69.92	GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC1110058...	[more]
A0A0A0KHF9	3.14e-34	57.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452010 PE=3 SV=1	[more]
A0A5D3CAN3	5.84e-34	56.45	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202...	[more]

BLAST of MC01g_new0304 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03610.1	6.8e-21	48.41	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT3G09930.1	2.8e-19	44.53	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G03600.1	2.7e-14	45.98	SGNH hydrolase-type esterase superfamily protein	[more]
AT2G36325.1	6.8e-13	45.56	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G20132.1	7.0e-10	36.11	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 25..131 e-value: 6.1E-15 score: 57.4
None	No IPR available	PANTHER	PTHR46020:SF4	OSJNBB0059K02.9 PROTEIN	coord: 15..124
None	No IPR available	PANTHER	PTHR46020	OSJNBB0059K02.9 PROTEIN	coord: 15..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g_new0304.1	MC01g_new0304.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds