MC01g_new0291 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0291 vs. ExPASy Swiss-Prot
Match: P45732 (Phenylalanine ammonia-lyase OS=Stylosanthes humilis OX=35628 GN=PAL17.1 PE=2 SV=1)

HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 595/693 (85.86%), Postives = 649/693 (93.65%), Query Frame = 0

Query: 58  HDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSDVVVELA 117
           HDPLNW  AAE+LKGSHLDEVKRMV EYR P+V LGG+TLTISQVAAIA  D  V V+L+
Sbjct: 23  HDPLNWAAAAEALKGSHLDEVKRMVSEYRKPLVNLGGQTLTISQVAAIAANDQGVSVQLS 82

Query: 118 ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGS 177
           E++RAGVKASSDWVM+SMN GTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG+
Sbjct: 83  EASRAGVKASSDWVMDSMNNGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGN 142

Query: 178 GAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLRGTITAS 237
           G E++ TLPH+A+RAAMLVRINTLLQGYSGIRFEILEAI  LLN+N+TPCLPLRGTITAS
Sbjct: 143 GTETNCTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITPCLPLRGTITAS 202

Query: 238 GDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLALVNGTA 297
           GDLVPLSYIAGLLTGRPNSKA+G NGETL+AKEAF+ AGI SDFFELQPKEGLALVNGT 
Sbjct: 203 GDLVPLSYIAGLLTGRPNSKAVGPNGETLNAKEAFQAAGIGSDFFELQPKEGLALVNGTP 262

Query: 298 VGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 357
           VGSGLAS+VLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH
Sbjct: 263 VGSGLASVVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 322

Query: 358 ILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVND 417
           ILDGSSY+KAAKKLHEIDPLQKPKQDRYALRTSPQWLGPL+EVIRFSTKSIEREINSVND
Sbjct: 323 ILDGSSYVKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLVEVIRFSTKSIEREINSVND 382

Query: 418 NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNL 477
           NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKLMFAQFSELVNDFYNNGLPSNL
Sbjct: 383 NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSELVNDFYNNGLPSNL 442

Query: 478 SASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLISSRKTAE 537
           SASRNPSLDYGFKG EIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS+RKT E
Sbjct: 443 SASRNPSLDYGFKGTEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISARKTNE 502

Query: 538 AIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGTLHPSRFCE 597
           A++ILKLMS T+L+ALCQAIDLRHLEENLK+TVK+TVSQVAK+ LTT  NG LHPSRFCE
Sbjct: 503 AVEILKLMSPTYLIALCQAIDLRHLEENLKNTVKNTVSQVAKRTLTTGVNGELHPSRFCE 562

Query: 598 KDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTSIFQKISAFE 657
           KDLLK+VDREY FAY+DD CSATYPLMQKLRQVLVEHAL N ++EKN NTSIFQKI+ FE
Sbjct: 563 KDLLKIVDREYCFAYIDDPCSATYPLMQKLRQVLVEHALANAENEKNVNTSIFQKITTFE 622

Query: 658 EELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLTGEKVISPGE 717
           EELK +L KEVE AR+A+E+G + I N+I+ECRSYPLY+FVREELGT +LTGEKV SPGE
Sbjct: 623 EELKTLLPKEVEGARIAYENGQSAIPNKIKECRSYPLYKFVREELGTEMLTGEKVRSPGE 682

Query: 718 ECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 751
           EC+K+FT +CQGK+ D +LEC+ EWNGAP+P+C
Sbjct: 683 ECDKLFTAMCQGKIIDPLLECIGEWNGAPLPLC 715

BLAST of MC01g_new0291 vs. ExPASy Swiss-Prot
Match: P45734 (Phenylalanine ammonia-lyase OS=Trifolium subterraneum OX=3900 GN=PAL1 PE=3 SV=1)

HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 599/697 (85.94%), Postives = 653/697 (93.69%), Query Frame = 0

Query: 54  KSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSDVV 113
           K +  DPLNWG+AAE++KGSHLDEVKRMVEEYR PVV+LGGETLTISQVAAIA  D    
Sbjct: 30  KVNAADPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIAAHDG-AT 89

Query: 114 VELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAG 173
           VEL+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAG
Sbjct: 90  VELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAG 149

Query: 174 IFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLRGT 233
           IFG+G ES+HTLPH+A+RAAMLVRINTLLQGYSGIRFEILEAI  LLN+N+TPCLPLRGT
Sbjct: 150 IFGNGTESNHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITPCLPLRGT 209

Query: 234 ITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLALV 293
           ITASGDLVPLSYIAGLLTGR NSKA G +GE L+AKEAF+ AGI+++FFELQPKEGLALV
Sbjct: 210 ITASGDLVPLSYIAGLLTGRSNSKAHGPSGEMLNAKEAFQLAGINAEFFELQPKEGLALV 269

Query: 294 NGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAA 353
           NGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAA
Sbjct: 270 NGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAA 329

Query: 354 IMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREIN 413
           IMEHIL GS+Y+K AKKLHE+DPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREIN
Sbjct: 330 IMEHILHGSAYVKDAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREIN 389

Query: 414 SVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGL 473
           SVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKL+FAQFSELVNDFYNNGL
Sbjct: 390 SVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVNDFYNNGL 449

Query: 474 PSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLISSR 533
           PSNLSASRNPSLDYGFKG+EIAMASYCSELQYLANPVT HVQSAEQHNQDVNSLGLISSR
Sbjct: 450 PSNLSASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISSR 509

Query: 534 KTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGTLHPS 593
           KT EAI+IL+LMSSTFL+ALCQAIDLRHLEENLK++VK+TVSQVAKK LT   +G LHPS
Sbjct: 510 KTKEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNTVSQVAKKTLTIGVSGELHPS 569

Query: 594 RFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTSIFQKI 653
           RFCEKDLLKVVDRE+ F+Y+DD CSATYPL QKLRQVLV+HAL NG+ EKN+NTSIFQKI
Sbjct: 570 RFCEKDLLKVVDREHVFSYIDDPCSATYPLAQKLRQVLVDHALVNGESEKNSNTSIFQKI 629

Query: 654 SAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLTGEKVI 713
           + FEEELK +L KEVE+AR A+E+GN+ I N+I  CRSYPLY+FVREELGTSLLTGE+VI
Sbjct: 630 ATFEEELKTLLPKEVESARTAYENGNSTIANKINGCRSYPLYKFVREELGTSLLTGERVI 689

Query: 714 SPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 751
           SPGEEC+K+FT +CQGK+ D +L+CL EWNGAP+PIC
Sbjct: 690 SPGEECDKLFTAMCQGKIIDPLLKCLGEWNGAPLPIC 725

BLAST of MC01g_new0291 vs. ExPASy Swiss-Prot
Match: P45730 (Phenylalanine ammonia-lyase OS=Populus trichocarpa OX=3694 GN=PAL PE=2 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 600/703 (85.35%), Postives = 654/703 (93.03%), Query Frame = 0

Query: 49  VETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATR 108
           +E++C +   DPL+WG+AAE++KGSHLDEVKRMV +YR PVVKLGGETLTI+QVA+IA  
Sbjct: 14  LESLCVNQ-RDPLSWGVAAEAMKGSHLDEVKRMVADYRKPVVKLGGETLTIAQVASIAGH 73

Query: 109 DS-DVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELI 168
           D+ DV VEL+ESAR GVKASSDWVM+SM+KGTDSYGVTTGFGATSHRRTKQGGALQKELI
Sbjct: 74  DTGDVKVELSESARPGVKASSDWVMDSMDKGTDSYGVTTGFGATSHRRTKQGGALQKELI 133

Query: 169 RFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPC 228
           RFLNAGIFG+G E+ HTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAI  LLN+N+TPC
Sbjct: 134 RFLNAGIFGNGTETCHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITRLLNNNITPC 193

Query: 229 LPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPK 288
           LPLRGTITASGDLVPLSYIAGLLTGRPNSKA G  GE LDA EAFK AGI S FFELQPK
Sbjct: 194 LPLRGTITASGDLVPLSYIAGLLTGRPNSKATGPTGEVLDAAEAFKAAGIESGFFELQPK 253

Query: 289 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 348
           EGLALVNGTAVGSGLAS+VLFE N+LAVLSE+LSAIFAEVM GKPEFTDHLTHKLKHHPG
Sbjct: 254 EGLALVNGTAVGSGLASMVLFETNVLAVLSELLSAIFAEVMNGKPEFTDHLTHKLKHHPG 313

Query: 349 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 408
           QIEAAAIMEHILDGS+YMKAAKKLHE DPLQKPKQDRYALRTSPQWLGP IEVIRFSTKS
Sbjct: 314 QIEAAAIMEHILDGSAYMKAAKKLHETDPLQKPKQDRYALRTSPQWLGPQIEVIRFSTKS 373

Query: 409 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 468
           IEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDN RLAIASIGKL+FAQFSELVND
Sbjct: 374 IEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNVRLAIASIGKLLFAQFSELVND 433

Query: 469 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSL 528
           FYNNGLPSNL+ASRNPSLDYGFKGAEIAMASYCSELQYLANPVT HVQSAEQHNQDVNSL
Sbjct: 434 FYNNGLPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSL 493

Query: 529 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 588
           GLISSRKTAEA+DILKLMS+TFLVALCQAIDLRHLEENLKS VK+TVSQV+K+VLTT +N
Sbjct: 494 GLISSRKTAEAVDILKLMSTTFLVALCQAIDLRHLEENLKSAVKNTVSQVSKRVLTTGAN 553

Query: 589 GTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNT 648
           G LHPSRFCEK+LLKVVDREY FAYVDD CSATYPLMQKLRQV V+HAL NG++EKN +T
Sbjct: 554 GELHPSRFCEKELLKVVDREYVFAYVDDPCSATYPLMQKLRQVFVDHALENGENEKNFST 613

Query: 649 SIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLL 708
           S+FQKI AFEEELKA+L KEVE+AR A++SGN+ I N+I+ECRSYPLY+FVREELGT LL
Sbjct: 614 SVFQKIEAFEEELKALLPKEVESARAAYDSGNSAIDNKIKECRSYPLYKFVREELGTVLL 673

Query: 709 TGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 751
           TGEKV SPGEE +K+FT +CQGK+ D +LECL EWNG+P+PIC
Sbjct: 674 TGEKVQSPGEEFDKVFTAMCQGKIIDPMLECLGEWNGSPLPIC 715

BLAST of MC01g_new0291 vs. ExPASy Swiss-Prot
Match: P27991 (Phenylalanine ammonia-lyase 1 OS=Glycine max OX=3847 GN=PAL1 PE=3 SV=1)

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 598/692 (86.42%), Postives = 647/692 (93.50%), Query Frame = 0

Query: 58  HDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSDVVVELA 117
           +DPLNWG AAE++KGSHLDEVKRMV EYR PVV+LGGETLTI+QVAA+A  D  V VEL+
Sbjct: 21  NDPLNWGAAAEAMKGSHLDEVKRMVAEYRKPVVRLGGETLTIAQVAAVAGHDHGVAVELS 80

Query: 118 ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGS 177
           ESAR GVKASS+WVM SMN GTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG+
Sbjct: 81  ESAREGVKASSEWVMNSMNNGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGN 140

Query: 178 GAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLRGTITAS 237
           G ESSHTLPH+A+RAAMLVRINTLLQGYSGIRFEILEAI  LLN+NVTPCL LRGTITAS
Sbjct: 141 GTESSHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNVTPCLDLRGTITAS 200

Query: 238 GDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLALVNGTA 297
           GDLVPLSYIAGLLTGRPNSKA+G +GE L+AKEAF+ A I+S+FFELQPKEGLALVNGTA
Sbjct: 201 GDLVPLSYIAGLLTGRPNSKAVGPSGEVLNAKEAFELASINSEFFELQPKEGLALVNGTA 260

Query: 298 VGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 357
           VGSGLAS+VLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH
Sbjct: 261 VGSGLASMVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 320

Query: 358 ILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVND 417
           ILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVND
Sbjct: 321 ILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVND 380

Query: 418 NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNL 477
           NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKLMFAQFSELVNDFYNNGLPSNL
Sbjct: 381 NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLMFAQFSELVNDFYNNGLPSNL 440

Query: 478 SASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLISSRKTAE 537
           +ASRNPSLDYGFKGAEIAMASYCSELQYLANPVT HVQSAEQHNQDVNSLGLISSRKT E
Sbjct: 441 TASRNPSLDYGFKGAEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISSRKTNE 500

Query: 538 AIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGTLHPSRFCE 597
           AI+ILKLMSSTFL+ALCQAIDLRHLEENLK++VK+TVSQV+K++LTT  NG LHPSRFCE
Sbjct: 501 AIEILKLMSSTFLIALCQAIDLRHLEENLKNSVKNTVSQVSKRILTTGVNGELHPSRFCE 560

Query: 598 KDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTSIFQKISAFE 657
           KDLLKVVDREY F+Y+DD CSATYPLMQKLRQVLV+HAL N + EK+ N+SIFQKI+ FE
Sbjct: 561 KDLLKVVDREYIFSYIDDPCSATYPLMQKLRQVLVDHALVNAECEKDVNSSIFQKIAIFE 620

Query: 658 EELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLTGEKVISPGE 717
           EELK +L KEVE AR A+ESG A I N+I+ECRSYPLY+FVREELGT LLTGEKV SPGE
Sbjct: 621 EELKNLLPKEVEGARAAYESGKAAIPNKIQECRSYPLYKFVREELGTGLLTGEKVRSPGE 680

Query: 718 ECEKIFTTLCQGKMTDSILECLKEWNGAPIPI 750
           E +K+FT +CQGK+ D ++ECL EWNGAP+PI
Sbjct: 681 EFDKLFTAMCQGKIIDPLMECLGEWNGAPLPI 712

BLAST of MC01g_new0291 vs. ExPASy Swiss-Prot
Match: Q01861 (Phenylalanine ammonia-lyase 1 OS=Pisum sativum OX=3888 GN=PAL1 PE=2 SV=1)

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 597/691 (86.40%), Postives = 648/691 (93.78%), Query Frame = 0

Query: 59  DPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSDVVVELAE 118
           DPLNWG+AAE++KGSHLDEVKRMVEEYR PVV+LGGETLTISQVAAIA  D  V VEL+E
Sbjct: 33  DPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIAAHDHGVKVELSE 92

Query: 119 SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSG 178
           SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG+G
Sbjct: 93  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 152

Query: 179 AESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLRGTITASG 238
            ESSHTLPH+A+RAAMLVRINTLLQGYSGIRFEILEAI  L+N+NVTPCL LRGTITASG
Sbjct: 153 TESSHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLINNNVTPCL-LRGTITASG 212

Query: 239 DLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLALVNGTAV 298
           DLVPLSYIAGLLTGRPNSKA G++GE L+AKEAF+ A I+  FFELQPKEGLALVNGTAV
Sbjct: 213 DLVPLSYIAGLLTGRPNSKAHGTSGEILNAKEAFQSAEINDGFFELQPKEGLALVNGTAV 272

Query: 299 GSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI 358
           GSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI
Sbjct: 273 GSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI 332

Query: 359 LDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDN 418
           LDGS+Y+KAAKKLHE+DPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDN
Sbjct: 333 LDGSAYVKAAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDN 392

Query: 419 PLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLS 478
           PLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKL+FAQFSELVNDFYNNGLPSNLS
Sbjct: 393 PLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVNDFYNNGLPSNLS 452

Query: 479 ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEA 538
           ASRNPSLDYGFKG+EIAMASYCSELQYLANPVT HVQSAEQHNQDVNSLGLISSRKT EA
Sbjct: 453 ASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISSRKTYEA 512

Query: 539 IDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGTLHPSRFCEK 598
           I+IL+LMSSTFL+ALCQA+DLRHLEENLK++VK+ VSQVAK+ LTT  NG LHPSRFCEK
Sbjct: 513 IEILQLMSSTFLIALCQAVDLRHLEENLKNSVKNIVSQVAKRTLTTGVNGELHPSRFCEK 572

Query: 599 DLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTSIFQKISAFEE 658
           DLL+VVDRE+ FAY+DD CSATYPLMQKLRQVLV+HAL NG+ EKN NTSIFQKI+ FE+
Sbjct: 573 DLLRVVDREHVFAYIDDPCSATYPLMQKLRQVLVDHALVNGESEKNLNTSIFQKIATFED 632

Query: 659 ELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLTGEKVISPGEE 718
           ELK +L KEVE+ R A+ESGN  + N+I  CRSYPLYRFVR+ELGT LLTGEKVISPGEE
Sbjct: 633 ELKTLLPKEVESTRAAYESGNPTVPNKINGCRSYPLYRFVRQELGTGLLTGEKVISPGEE 692

Query: 719 CEKIFTTLCQGKMTDSILECLKEWNGAPIPI 750
           C+K+FT +CQGK+ D +L+CL +WNGAP+PI
Sbjct: 693 CDKLFTAICQGKIIDPLLQCLGDWNGAPLPI 722

BLAST of MC01g_new0291 vs. NCBI nr
Match: XP_022143840.1 (phenylalanine ammonia-lyase-like [Momordica charantia])

HSP 1 Score: 1362 bits (3525), Expect = 0.0
Identity = 704/705 (99.86%), Postives = 704/705 (99.86%), Query Frame = 0

Query: 46  MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI 105
           MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI
Sbjct: 1   MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI 60

Query: 106 ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 165
           ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE
Sbjct: 61  ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 120

Query: 166 LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT 225
           LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT
Sbjct: 121 LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT 180

Query: 226 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ 285
           PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ
Sbjct: 181 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ 240

Query: 286 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 345
           PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH
Sbjct: 241 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 300

Query: 346 PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 405
           PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST
Sbjct: 301 PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 360

Query: 406 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV 465
           KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV
Sbjct: 361 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV 420

Query: 466 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN 525
           NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN
Sbjct: 421 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN 480

Query: 526 SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTS 585
           SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKS VSQVAKKVLTTS
Sbjct: 481 SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSXVSQVAKKVLTTS 540

Query: 586 SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT 645
           SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT
Sbjct: 541 SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT 600

Query: 646 NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS 705
           NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS
Sbjct: 601 NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS 660

Query: 706 LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC
Sbjct: 661 LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 705

BLAST of MC01g_new0291 vs. NCBI nr
Match: XP_022977757.1 (phenylalanine ammonia-lyase-like [Cucurbita maxima])

HSP 1 Score: 1289 bits (3336), Expect = 0.0
Identity = 657/705 (93.19%), Postives = 680/705 (96.45%), Query Frame = 0

Query: 46  MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI 105
           MA +E ICKSS  DPLNWG+AA+SLKGSHLDEVKRMVEEYRSP+VKLGG+TLTISQVAAI
Sbjct: 1   MAPIEPICKSSTSDPLNWGVAADSLKGSHLDEVKRMVEEYRSPLVKLGGQTLTISQVAAI 60

Query: 106 ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 165
           ATRDSDV VELAES+RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRT QGGALQKE
Sbjct: 61  ATRDSDVTVELAESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTNQGGALQKE 120

Query: 166 LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT 225
           LIRFLNAGIFGSGAES HTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAI  LLNHN+T
Sbjct: 121 LIRFLNAGIFGSGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNIT 180

Query: 226 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ 285
           PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+G NGE LDAKEAF+QA I S FFELQ
Sbjct: 181 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGERLDAKEAFQQAAIPSGFFELQ 240

Query: 286 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 345
           PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH
Sbjct: 241 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 300

Query: 346 PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 405
           PGQIEAAAIMEHILDGSSYMK AK+LHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST
Sbjct: 301 PGQIEAAAIMEHILDGSSYMKTAKELHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 360

Query: 406 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV 465
           KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKLMFAQFSELV
Sbjct: 361 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSELV 420

Query: 466 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN 525
           NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVN
Sbjct: 421 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVN 480

Query: 526 SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTS 585
           SLGLISSRKTAEAIDILKLM STFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTT 
Sbjct: 481 SLGLISSRKTAEAIDILKLMCSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTG 540

Query: 586 SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT 645
           +NG LHPSRFCEKDLLKVVDREYTFAYVDDACS TYPLMQKLRQVLVEHAL NGD E+NT
Sbjct: 541 TNGALHPSRFCEKDLLKVVDREYTFAYVDDACSGTYPLMQKLRQVLVEHALANGDGEQNT 600

Query: 646 NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS 705
           NTSIFQKI+AFEEELKA+L KEVEN RLA+ESGNA+IKNQIEECRSYPLYRFVR+ELGT+
Sbjct: 601 NTSIFQKIAAFEEELKAVLPKEVENTRLAYESGNAQIKNQIEECRSYPLYRFVRKELGTN 660

Query: 706 LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           L+TGEKVISPGEECEK+FT LCQGKM DSILECLKEWNGAPIPIC
Sbjct: 661 LVTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 705

BLAST of MC01g_new0291 vs. NCBI nr
Match: XP_022154420.1 (phenylalanine ammonia-lyase-like [Momordica charantia])

HSP 1 Score: 1288 bits (3333), Expect = 0.0
Identity = 661/703 (94.03%), Postives = 683/703 (97.16%), Query Frame = 0

Query: 48  QVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIAT 107
           Q E+ C  S  DPLNWG+AA+SLKGSHLDEVKRMVEEYR PVVKLGGETLTISQVAAIAT
Sbjct: 9   QKESFCSISPQDPLNWGLAADSLKGSHLDEVKRMVEEYRRPVVKLGGETLTISQVAAIAT 68

Query: 108 RDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELI 167
           RDSDV+VELAESARAGVKASSDWVMESM+KGTDSYGVTTGFGATSHRRTKQGGALQKELI
Sbjct: 69  RDSDVIVELAESARAGVKASSDWVMESMSKGTDSYGVTTGFGATSHRRTKQGGALQKELI 128

Query: 168 RFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPC 227
           RFLNAGIFG+GAESSHTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAIA+LLNHNVTPC
Sbjct: 129 RFLNAGIFGNGAESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIANLLNHNVTPC 188

Query: 228 LPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPK 287
           LPLRGTITASGDLVPLSYIAGLLTGR NSKAIG NGETLDA+ AFKQA I SDFFELQPK
Sbjct: 189 LPLRGTITASGDLVPLSYIAGLLTGRHNSKAIGPNGETLDAEAAFKQACIPSDFFELQPK 248

Query: 288 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 347
           EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG
Sbjct: 249 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 308

Query: 348 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 407
           QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS
Sbjct: 309 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 368

Query: 408 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 467
           IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND
Sbjct: 369 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 428

Query: 468 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSL 527
           FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSL
Sbjct: 429 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSL 488

Query: 528 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 587
           GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN
Sbjct: 489 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 548

Query: 588 GTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNT 647
           G LHPSRFCEKDLLKVVDREYTFAY+DD CSATYPLMQKLRQVLVEHAL+NGD EK+TNT
Sbjct: 549 GALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQVLVEHALSNGDGEKSTNT 608

Query: 648 SIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLL 707
           SIFQKI AFEEELKA+L +EVENARLA+ESGN+KIKNQI++CRSYPLYRFVREELGTSLL
Sbjct: 609 SIFQKIGAFEEELKAVLPREVENARLAYESGNSKIKNQIKDCRSYPLYRFVREELGTSLL 668

Query: 708 TGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           TGEKVISPGEECEK+FT LC+GKM DSI+ECLKEWNGAPI IC
Sbjct: 669 TGEKVISPGEECEKVFTALCEGKMIDSIIECLKEWNGAPILIC 711

BLAST of MC01g_new0291 vs. NCBI nr
Match: XP_022154421.1 (phenylalanine ammonia-lyase-like [Momordica charantia])

HSP 1 Score: 1285 bits (3326), Expect = 0.0
Identity = 660/703 (93.88%), Postives = 682/703 (97.01%), Query Frame = 0

Query: 48  QVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIAT 107
           Q E+ C  S  DPLNWG+AA+SLKGSHLDEVKRMVEEYR PVVKLGGETLTISQVAAIAT
Sbjct: 9   QKESFCSISPQDPLNWGLAADSLKGSHLDEVKRMVEEYRRPVVKLGGETLTISQVAAIAT 68

Query: 108 RDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELI 167
           RDSDV+VELAESARA VKASSDWVMESM+KGTDSYGVTTGFGATSHRRTKQGGALQKELI
Sbjct: 69  RDSDVIVELAESARAXVKASSDWVMESMSKGTDSYGVTTGFGATSHRRTKQGGALQKELI 128

Query: 168 RFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPC 227
           RFLNAGIFG+GAESSHTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAIA+LLNHNVTPC
Sbjct: 129 RFLNAGIFGNGAESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIANLLNHNVTPC 188

Query: 228 LPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPK 287
           LPLRGTITASGDLVPLSYIAGLLTGR NSKAIG NGETLDA+ AFKQA I SDFFELQPK
Sbjct: 189 LPLRGTITASGDLVPLSYIAGLLTGRHNSKAIGPNGETLDAEAAFKQACIPSDFFELQPK 248

Query: 288 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 347
           EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG
Sbjct: 249 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 308

Query: 348 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 407
           QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS
Sbjct: 309 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 368

Query: 408 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 467
           IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND
Sbjct: 369 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 428

Query: 468 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSL 527
           FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSL
Sbjct: 429 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSL 488

Query: 528 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 587
           GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN
Sbjct: 489 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 548

Query: 588 GTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNT 647
           G LHPSRFCEKDLLKVVDREYTFAY+DD CSATYPLMQKLRQVLVEHAL+NGD EK+TNT
Sbjct: 549 GALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQVLVEHALSNGDGEKSTNT 608

Query: 648 SIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLL 707
           SIFQKI AFEEELKA+L +EVENARLA+ESGN+KIKNQI++CRSYPLYRFVREELGTSLL
Sbjct: 609 SIFQKIGAFEEELKAVLPREVENARLAYESGNSKIKNQIKDCRSYPLYRFVREELGTSLL 668

Query: 708 TGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           TGEKVISPGEECEK+FT LC+GKM DSI+ECLKEWNGAPI IC
Sbjct: 669 TGEKVISPGEECEKVFTALCEGKMIDSIIECLKEWNGAPILIC 711

BLAST of MC01g_new0291 vs. NCBI nr
Match: XP_038881051.1 (phenylalanine ammonia-lyase-like [Benincasa hispida])

HSP 1 Score: 1281 bits (3316), Expect = 0.0
Identity = 652/705 (92.48%), Postives = 678/705 (96.17%), Query Frame = 0

Query: 46  MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI 105
           MA +ETICKS IHDPLNWG+AAESLKGSHLDEVKRMVEEYR P++KLGGETLTISQVAAI
Sbjct: 1   MAPIETICKSVIHDPLNWGIAAESLKGSHLDEVKRMVEEYRRPLIKLGGETLTISQVAAI 60

Query: 106 ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 165
           ATR+SDV VEL+ESARAGV ASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE
Sbjct: 61  ATRESDVTVELSESARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 120

Query: 166 LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT 225
           LIRFLNAGIFG+G ESSHTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAI  LLNHN+T
Sbjct: 121 LIRFLNAGIFGNGTESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNIT 180

Query: 226 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ 285
           PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+G NGE+LDAKEAF+QA I S FFELQ
Sbjct: 181 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAKEAFQQADIPSGFFELQ 240

Query: 286 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 345
           PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH
Sbjct: 241 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 300

Query: 346 PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 405
           PGQIEAAAIMEHILDGSSYMK AKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST
Sbjct: 301 PGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 360

Query: 406 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV 465
           KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV
Sbjct: 361 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV 420

Query: 466 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN 525
           NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVN
Sbjct: 421 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVN 480

Query: 526 SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTS 585
           SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTT 
Sbjct: 481 SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTC 540

Query: 586 SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT 645
           SNG LHPSRFCEKDLLKVVDREYTFAY+DD C+ATYPL QKLRQVLVEHAL NGDDEKN 
Sbjct: 541 SNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNA 600

Query: 646 NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS 705
           +TSIFQKI  FEEELKA+L KEVENARLA+E+GN+KI+NQI++CRSYP+YRFVREEL T 
Sbjct: 601 DTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETK 660

Query: 706 LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           +LTGEKVISPGEECEK+F  LCQGKM DSILECLKEWNGAPIPIC
Sbjct: 661 MLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 705

BLAST of MC01g_new0291 vs. ExPASy TrEMBL
Match: A0A6J1CPY0 (Phenylalanine ammonia-lyase OS=Momordica charantia OX=3673 GN=LOC111013660 PE=3 SV=1)

HSP 1 Score: 1362 bits (3525), Expect = 0.0
Identity = 704/705 (99.86%), Postives = 704/705 (99.86%), Query Frame = 0

Query: 46  MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI 105
           MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI
Sbjct: 1   MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI 60

Query: 106 ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 165
           ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE
Sbjct: 61  ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 120

Query: 166 LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT 225
           LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT
Sbjct: 121 LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT 180

Query: 226 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ 285
           PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ
Sbjct: 181 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ 240

Query: 286 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 345
           PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH
Sbjct: 241 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 300

Query: 346 PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 405
           PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST
Sbjct: 301 PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 360

Query: 406 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV 465
           KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV
Sbjct: 361 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV 420

Query: 466 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN 525
           NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN
Sbjct: 421 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN 480

Query: 526 SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTS 585
           SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKS VSQVAKKVLTTS
Sbjct: 481 SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSXVSQVAKKVLTTS 540

Query: 586 SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT 645
           SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT
Sbjct: 541 SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT 600

Query: 646 NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS 705
           NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS
Sbjct: 601 NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS 660

Query: 706 LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC
Sbjct: 661 LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 705

BLAST of MC01g_new0291 vs. ExPASy TrEMBL
Match: A0A6J1IJC7 (Phenylalanine ammonia-lyase OS=Cucurbita maxima OX=3661 GN=LOC111477963 PE=3 SV=1)

HSP 1 Score: 1289 bits (3336), Expect = 0.0
Identity = 657/705 (93.19%), Postives = 680/705 (96.45%), Query Frame = 0

Query: 46  MAQVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAI 105
           MA +E ICKSS  DPLNWG+AA+SLKGSHLDEVKRMVEEYRSP+VKLGG+TLTISQVAAI
Sbjct: 1   MAPIEPICKSSTSDPLNWGVAADSLKGSHLDEVKRMVEEYRSPLVKLGGQTLTISQVAAI 60

Query: 106 ATRDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 165
           ATRDSDV VELAES+RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRT QGGALQKE
Sbjct: 61  ATRDSDVTVELAESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTNQGGALQKE 120

Query: 166 LIRFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVT 225
           LIRFLNAGIFGSGAES HTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAI  LLNHN+T
Sbjct: 121 LIRFLNAGIFGSGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNIT 180

Query: 226 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQ 285
           PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+G NGE LDAKEAF+QA I S FFELQ
Sbjct: 181 PCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGERLDAKEAFQQAAIPSGFFELQ 240

Query: 286 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 345
           PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH
Sbjct: 241 PKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHH 300

Query: 346 PGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 405
           PGQIEAAAIMEHILDGSSYMK AK+LHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST
Sbjct: 301 PGQIEAAAIMEHILDGSSYMKTAKELHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFST 360

Query: 406 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELV 465
           KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKLMFAQFSELV
Sbjct: 361 KSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSELV 420

Query: 466 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVN 525
           NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVN
Sbjct: 421 NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVN 480

Query: 526 SLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTS 585
           SLGLISSRKTAEAIDILKLM STFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTT 
Sbjct: 481 SLGLISSRKTAEAIDILKLMCSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTG 540

Query: 586 SNGTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNT 645
           +NG LHPSRFCEKDLLKVVDREYTFAYVDDACS TYPLMQKLRQVLVEHAL NGD E+NT
Sbjct: 541 TNGALHPSRFCEKDLLKVVDREYTFAYVDDACSGTYPLMQKLRQVLVEHALANGDGEQNT 600

Query: 646 NTSIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTS 705
           NTSIFQKI+AFEEELKA+L KEVEN RLA+ESGNA+IKNQIEECRSYPLYRFVR+ELGT+
Sbjct: 601 NTSIFQKIAAFEEELKAVLPKEVENTRLAYESGNAQIKNQIEECRSYPLYRFVRKELGTN 660

Query: 706 LLTGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           L+TGEKVISPGEECEK+FT LCQGKM DSILECLKEWNGAPIPIC
Sbjct: 661 LVTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 705

BLAST of MC01g_new0291 vs. ExPASy TrEMBL
Match: A0A6J1DJJ7 (Phenylalanine ammonia-lyase OS=Momordica charantia OX=3673 GN=LOC111021692 PE=3 SV=1)

HSP 1 Score: 1288 bits (3333), Expect = 0.0
Identity = 661/703 (94.03%), Postives = 683/703 (97.16%), Query Frame = 0

Query: 48  QVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIAT 107
           Q E+ C  S  DPLNWG+AA+SLKGSHLDEVKRMVEEYR PVVKLGGETLTISQVAAIAT
Sbjct: 9   QKESFCSISPQDPLNWGLAADSLKGSHLDEVKRMVEEYRRPVVKLGGETLTISQVAAIAT 68

Query: 108 RDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELI 167
           RDSDV+VELAESARAGVKASSDWVMESM+KGTDSYGVTTGFGATSHRRTKQGGALQKELI
Sbjct: 69  RDSDVIVELAESARAGVKASSDWVMESMSKGTDSYGVTTGFGATSHRRTKQGGALQKELI 128

Query: 168 RFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPC 227
           RFLNAGIFG+GAESSHTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAIA+LLNHNVTPC
Sbjct: 129 RFLNAGIFGNGAESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIANLLNHNVTPC 188

Query: 228 LPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPK 287
           LPLRGTITASGDLVPLSYIAGLLTGR NSKAIG NGETLDA+ AFKQA I SDFFELQPK
Sbjct: 189 LPLRGTITASGDLVPLSYIAGLLTGRHNSKAIGPNGETLDAEAAFKQACIPSDFFELQPK 248

Query: 288 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 347
           EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG
Sbjct: 249 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 308

Query: 348 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 407
           QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS
Sbjct: 309 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 368

Query: 408 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 467
           IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND
Sbjct: 369 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 428

Query: 468 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSL 527
           FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSL
Sbjct: 429 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSL 488

Query: 528 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 587
           GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN
Sbjct: 489 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 548

Query: 588 GTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNT 647
           G LHPSRFCEKDLLKVVDREYTFAY+DD CSATYPLMQKLRQVLVEHAL+NGD EK+TNT
Sbjct: 549 GALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQVLVEHALSNGDGEKSTNT 608

Query: 648 SIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLL 707
           SIFQKI AFEEELKA+L +EVENARLA+ESGN+KIKNQI++CRSYPLYRFVREELGTSLL
Sbjct: 609 SIFQKIGAFEEELKAVLPREVENARLAYESGNSKIKNQIKDCRSYPLYRFVREELGTSLL 668

Query: 708 TGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           TGEKVISPGEECEK+FT LC+GKM DSI+ECLKEWNGAPI IC
Sbjct: 669 TGEKVISPGEECEKVFTALCEGKMIDSIIECLKEWNGAPILIC 711

BLAST of MC01g_new0291 vs. ExPASy TrEMBL
Match: A0A6J1DNP0 (Phenylalanine ammonia-lyase OS=Momordica charantia OX=3673 GN=LOC111021693 PE=3 SV=1)

HSP 1 Score: 1285 bits (3326), Expect = 0.0
Identity = 660/703 (93.88%), Postives = 682/703 (97.01%), Query Frame = 0

Query: 48  QVETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIAT 107
           Q E+ C  S  DPLNWG+AA+SLKGSHLDEVKRMVEEYR PVVKLGGETLTISQVAAIAT
Sbjct: 9   QKESFCSISPQDPLNWGLAADSLKGSHLDEVKRMVEEYRRPVVKLGGETLTISQVAAIAT 68

Query: 108 RDSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELI 167
           RDSDV+VELAESARA VKASSDWVMESM+KGTDSYGVTTGFGATSHRRTKQGGALQKELI
Sbjct: 69  RDSDVIVELAESARAXVKASSDWVMESMSKGTDSYGVTTGFGATSHRRTKQGGALQKELI 128

Query: 168 RFLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPC 227
           RFLNAGIFG+GAESSHTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAIA+LLNHNVTPC
Sbjct: 129 RFLNAGIFGNGAESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIANLLNHNVTPC 188

Query: 228 LPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPK 287
           LPLRGTITASGDLVPLSYIAGLLTGR NSKAIG NGETLDA+ AFKQA I SDFFELQPK
Sbjct: 189 LPLRGTITASGDLVPLSYIAGLLTGRHNSKAIGPNGETLDAEAAFKQACIPSDFFELQPK 248

Query: 288 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 347
           EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG
Sbjct: 249 EGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPG 308

Query: 348 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 407
           QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS
Sbjct: 309 QIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKS 368

Query: 408 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 467
           IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND
Sbjct: 369 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 428

Query: 468 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSL 527
           FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSL
Sbjct: 429 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSL 488

Query: 528 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 587
           GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN
Sbjct: 489 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 548

Query: 588 GTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNT 647
           G LHPSRFCEKDLLKVVDREYTFAY+DD CSATYPLMQKLRQVLVEHAL+NGD EK+TNT
Sbjct: 549 GALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQVLVEHALSNGDGEKSTNT 608

Query: 648 SIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLL 707
           SIFQKI AFEEELKA+L +EVENARLA+ESGN+KIKNQI++CRSYPLYRFVREELGTSLL
Sbjct: 609 SIFQKIGAFEEELKAVLPREVENARLAYESGNSKIKNQIKDCRSYPLYRFVREELGTSLL 668

Query: 708 TGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           TGEKVISPGEECEK+FT LC+GKM DSI+ECLKEWNGAPI IC
Sbjct: 669 TGEKVISPGEECEKVFTALCEGKMIDSIIECLKEWNGAPILIC 711

BLAST of MC01g_new0291 vs. ExPASy TrEMBL
Match: A0A5D3C1Q6 (Phenylalanine ammonia-lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold432G00140 PE=3 SV=1)

HSP 1 Score: 1280 bits (3313), Expect = 0.0
Identity = 652/702 (92.88%), Postives = 680/702 (96.87%), Query Frame = 0

Query: 49  VETICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATR 108
           +E+ C S + DPLNWG+AAE+LKGSHLDEVKRMV+EYR PVVKLGGETLTISQVAAIATR
Sbjct: 16  MESFCTSHVVDPLNWGVAAEALKGSHLDEVKRMVDEYRRPVVKLGGETLTISQVAAIATR 75

Query: 109 DSDVVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR 168
           DSDVVV+LAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR
Sbjct: 76  DSDVVVKLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR 135

Query: 169 FLNAGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCL 228
           FLNAGIFG+GAES+HTLPHSA+RAAMLVRINTLLQGYSGIRFEILEAI +LLN NVTPCL
Sbjct: 136 FLNAGIFGNGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITNLLNENVTPCL 195

Query: 229 PLRGTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKE 288
           PLRGTITASGDLVPLSYIAGLLTGRPNSKAIG NGETLDA+ AFKQAGI S FFELQPKE
Sbjct: 196 PLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGETLDAEAAFKQAGIPSGFFELQPKE 255

Query: 289 GLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQ 348
           GLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPE+TDHLTHKLKHHPGQ
Sbjct: 256 GLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEYTDHLTHKLKHHPGQ 315

Query: 349 IEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSI 408
           IEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSI
Sbjct: 316 IEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSI 375

Query: 409 EREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDF 468
           EREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDF
Sbjct: 376 EREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDF 435

Query: 469 YNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLG 528
           YNNGLPSNLS SRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSLG
Sbjct: 436 YNNGLPSNLSGSRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLG 495

Query: 529 LISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNG 588
           LISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKS VSQVAKKVLTTSSNG
Sbjct: 496 LISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSIVSQVAKKVLTTSSNG 555

Query: 589 TLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTS 648
           TLHPSRFCEKDLLKVVDREYTFAY+DD CSATYPLMQKLRQVLV+HALTNG++EKNTNTS
Sbjct: 556 TLHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQVLVDHALTNGENEKNTNTS 615

Query: 649 IFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLT 708
           IFQKI+AFEEELK  L KEVENARLA+ESGN K+ NQI++CRSYPLY+FVRE+LGT LLT
Sbjct: 616 IFQKITAFEEELKVALPKEVENARLAYESGNCKVANQIKDCRSYPLYKFVREDLGTKLLT 675

Query: 709 GEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 750
           GE+VISPGEECEK+FT LCQGKM DSILECLKEWNGAPIPIC
Sbjct: 676 GERVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717

BLAST of MC01g_new0291 vs. TAIR 10
Match: AT2G37040.1 (PHE ammonia lyase 1 )

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 582/692 (84.10%), Postives = 636/692 (91.91%), Query Frame = 0

Query: 59  DPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSDVVVELAE 118
           DPLNWG AAE +KGSHLDEVKRMV E+R PVV LGGETLTI QVAAI+T  + V VEL+E
Sbjct: 34  DPLNWGAAAEQMKGSHLDEVKRMVAEFRKPVVNLGGETLTIGQVAAISTIGNSVKVELSE 93

Query: 119 SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSG 178
           +ARAGV ASSDWVMESMNKGTDSYGVTTGFGATSHRRTK G ALQKELIRFLNAGIFGS 
Sbjct: 94  TARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFLNAGIFGST 153

Query: 179 AESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLRGTITASG 238
            E+SHTLPHSA+RAAMLVRINTLLQG+SGIRFEILEAI S LN+N+TP LPLRGTITASG
Sbjct: 154 KETSHTLPHSATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPLRGTITASG 213

Query: 239 DLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLALVNGTAV 298
           DLVPLSYIAGLLTGRPNSKA G NGE L A+EAFK AGISS FF+LQPKEGLALVNGTAV
Sbjct: 214 DLVPLSYIAGLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPKEGLALVNGTAV 273

Query: 299 GSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI 358
           GSG+AS+VLFE N+L+VL+EILSA+FAEVM GKPEFTDHLTH+LKHHPGQIEAAAIMEHI
Sbjct: 274 GSGMASMVLFETNVLSVLAEILSAVFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHI 333

Query: 359 LDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDN 418
           LDGSSYMK A+KLHE+DPLQKPKQDRYALRTSPQWLGP IEVIR++TKSIEREINSVNDN
Sbjct: 334 LDGSSYMKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRYATKSIEREINSVNDN 393

Query: 419 PLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLS 478
           PLIDVSRNKA+HGGNFQGTPIGVSMDNTRLAIA+IGKLMFAQFSELVNDFYNNGLPSNL+
Sbjct: 394 PLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLT 453

Query: 479 ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEA 538
           ASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSLGLISSRKT+EA
Sbjct: 454 ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEA 513

Query: 539 IDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGTLHPSRFCEK 598
           +DILKLMS+TFLVA+CQA+DLRHLEENL+ TVK+TVSQVAKKVLTT  NG LHPSRFCEK
Sbjct: 514 VDILKLMSTTFLVAICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGELHPSRFCEK 573

Query: 599 DLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTSIFQKISAFEE 658
           DLLKVVDRE  + Y DD CSATYPL+QKLRQV+V+HAL NG+ EKN  TSIF KI AFEE
Sbjct: 574 DLLKVVDREQVYTYADDPCSATYPLIQKLRQVIVDHALINGESEKNAVTSIFHKIGAFEE 633

Query: 659 ELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLTGEKVISPGEE 718
           ELKA+L KEVE AR A+++G + I N+I+ECRSYPLYRFVREELGT LLTGEKV SPGEE
Sbjct: 634 ELKAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELLTGEKVTSPGEE 693

Query: 719 CEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 751
            +K+FT +C+GK+ D ++ECL EWNGAPIPIC
Sbjct: 694 FDKVFTAICEGKIIDPMMECLNEWNGAPIPIC 725

BLAST of MC01g_new0291 vs. TAIR 10
Match: AT3G53260.1 (phenylalanine ammonia-lyase 2 )

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 580/699 (82.98%), Postives = 642/699 (91.85%), Query Frame = 0

Query: 52  ICKSSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSD 111
           +   ++ DPLNWG+AA+ +KGSHLDEVK+MVEEYR PVV LGGETLTI QVAAI+T    
Sbjct: 19  VTTKTLADPLNWGLAADQMKGSHLDEVKKMVEEYRRPVVNLGGETLTIGQVAAISTVGGS 78

Query: 112 VVVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLN 171
           V VELAE++RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK G ALQ ELIRFLN
Sbjct: 79  VKVELAETSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLN 138

Query: 172 AGIFGSGAESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLR 231
           AGIFG+  E+ HTLP SA+RAAMLVR+NTLLQGYSGIRFEILEAI SLLNHN++P LPLR
Sbjct: 139 AGIFGNTKETCHTLPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLR 198

Query: 232 GTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLA 291
           GTITASGDLVPLSYIAGLLTGRPNSKA G +GE+L AKEAF++AGIS+ FF+LQPKEGLA
Sbjct: 199 GTITASGDLVPLSYIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPKEGLA 258

Query: 292 LVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEA 351
           LVNGTAVGSG+AS+VLFEAN+ AVL+E+LSAIFAEVM GKPEFTDHLTH+LKHHPGQIEA
Sbjct: 259 LVNGTAVGSGMASMVLFEANVQAVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEA 318

Query: 352 AAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIERE 411
           AAIMEHILDGSSYMK A+K+HE+DPLQKPKQDRYALRTSPQWLGP IEVIR +TKSIERE
Sbjct: 319 AAIMEHILDGSSYMKLAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIERE 378

Query: 412 INSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNN 471
           INSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLAIA+IGKLMFAQFSELVNDFYNN
Sbjct: 379 INSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNN 438

Query: 472 GLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLIS 531
           GLPSNL+AS NPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS
Sbjct: 439 GLPSNLTASSNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLIS 498

Query: 532 SRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGTLH 591
           SRKT+EA+DILKLMS+TFLV +CQA+DLRHLEENL+ TVK+TVSQVAKKVLTT  NG LH
Sbjct: 499 SRKTSEAVDILKLMSTTFLVGICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELH 558

Query: 592 PSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTSIFQ 651
           PSRFCEKDLLKVVDRE  F YVDD CSATYPLMQ+LRQV+V+HAL+NG+ EKN  TSIFQ
Sbjct: 559 PSRFCEKDLLKVVDREQVFTYVDDPCSATYPLMQRLRQVIVDHALSNGETEKNAVTSIFQ 618

Query: 652 KISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLTGEK 711
           KI AFEEELKA+L KEVE AR A+ +G A I N+I+ECRSYPLYRFVREELGT LLTGEK
Sbjct: 619 KIGAFEEELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEK 678

Query: 712 VISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 751
           V+SPGEE +K+FT +C+GK+ D +++CLKEWNGAPIPIC
Sbjct: 679 VVSPGEEFDKVFTAMCEGKLIDPLMDCLKEWNGAPIPIC 717

BLAST of MC01g_new0291 vs. TAIR 10
Match: AT3G10340.1 (phenylalanine ammonia-lyase 4 )

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 562/693 (81.10%), Postives = 615/693 (88.74%), Query Frame = 0

Query: 59  DPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSDVVVELAE 118
           DPLNW   AE+LKGSHLDEVKRMV+EYR   VKLGGETLTI QVAA+A       VELAE
Sbjct: 16  DPLNWNATAEALKGSHLDEVKRMVKEYRKEAVKLGGETLTIGQVAAVARGGGGSTVELAE 75

Query: 119 SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSG 178
            ARAGVKASS+WVMESMN+GTDSYGVTTGFGATSHRRTKQGGALQ ELIRFLNAGIFG G
Sbjct: 76  EARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQNELIRFLNAGIFGPG 135

Query: 179 A-ESSHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLRGTITAS 238
           A ++SHTLP   +RAAMLVR+NTLLQGYSGIRFEILEAI  LLNH +TPCLPLRGTITAS
Sbjct: 136 AGDTSHTLPKPTTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEITPCLPLRGTITAS 195

Query: 239 GDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLALVNGTA 298
           GDLVPLSYIAGLLTGRPNSKA+G +GETL A EAFK AG+SS FFELQPKEGLALVNGTA
Sbjct: 196 GDLVPLSYIAGLLTGRPNSKAVGPSGETLTASEAFKLAGVSS-FFELQPKEGLALVNGTA 255

Query: 299 VGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 358
           VGSGLAS VLF+ANILAVLSE++SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH
Sbjct: 256 VGSGLASTVLFDANILAVLSEVMSAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 315

Query: 359 ILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVND 418
           ILDGSSY+K A+ LHE+DPLQKPKQDRYALRTSPQWLGP IEVIR +TK IEREINSVND
Sbjct: 316 ILDGSSYVKEAQLLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVND 375

Query: 419 NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNL 478
           NPLIDVSRNKALHGGNFQGTPIGV+MDN+RLAIASIGKLMFAQFSELVNDFYNNGLPSNL
Sbjct: 376 NPLIDVSRNKALHGGNFQGTPIGVAMDNSRLAIASIGKLMFAQFSELVNDFYNNGLPSNL 435

Query: 479 SASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLISSRKTAE 538
           S  RNPSLDYGFKGAEIAMASYCSELQ+LANPVTNHVQSAEQHNQDVNSLGLISSRKTAE
Sbjct: 436 SGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAE 495

Query: 539 AIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGTLHPSRFCE 598
           A+DILKLMS+T+LVALCQA+DLRHLEENLK  VKS VSQVAK+VLT  +NG LHPSRF E
Sbjct: 496 AVDILKLMSTTYLVALCQAVDLRHLEENLKKAVKSAVSQVAKRVLTVGANGELHPSRFTE 555

Query: 599 KDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTSIFQKISAFE 658
           +D+L+VVDREY F+Y DD CS TYPLMQKLR +LV+HAL + + E N+ TS+F KI AFE
Sbjct: 556 RDVLQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHALADPEREANSATSVFHKIGAFE 615

Query: 659 EELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLTGEKVISPGE 718
            ELK +L KEVE  R+ +E G + I N+I+ECRSYPLYRFVR+EL T LLTGE V SPGE
Sbjct: 616 AELKLLLPKEVERVRVEYEEGTSAIANRIKECRSYPLYRFVRDELNTELLTGENVRSPGE 675

Query: 719 ECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 751
           E +K+F  +  GK+ D +LECLKEWNGAP+ IC
Sbjct: 676 EFDKVFLAISDGKLIDPLLECLKEWNGAPVSIC 707

BLAST of MC01g_new0291 vs. TAIR 10
Match: AT5G04230.1 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 977.6 bits (2526), Expect = 5.5e-285
Identity = 508/699 (72.68%), Postives = 579/699 (82.83%), Query Frame = 0

Query: 55  SSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSDVVV 114
           +++ DPLNW +AAE+LKGSHL+EVK+MV++YR   V+LGGETLTI QVAA+A+      V
Sbjct: 9   TALSDPLNWNVAAEALKGSHLEEVKKMVKDYRKGTVQLGGETLTIGQVAAVAS--GGPTV 68

Query: 115 ELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGI 174
           EL+E AR GVKASSDWVMESMN+ TD+YG+TTGFG++S RRT QG ALQKELIR+LNAGI
Sbjct: 69  ELSEEARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGI 128

Query: 175 FGSGAES---SHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLR 234
           F +G E    S+TLP  A+RAAML+R+NTLLQGYSGIRFEILEAI +LLN  +TP LPLR
Sbjct: 129 FATGNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLR 188

Query: 235 GTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLA 294
           GTITASGDLVPLSYIAG L GRPNS+++G +GE L A EAFK AG+SS FFEL+PKEGLA
Sbjct: 189 GTITASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVSS-FFELRPKEGLA 248

Query: 295 LVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEA 354
           LVNGTAVGS LAS VL++ANIL V SE+ SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEA
Sbjct: 249 LVNGTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEA 308

Query: 355 AAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIERE 414
           AAIMEHILDGSSY+K A  LH+IDPLQKPKQDRYALRTSPQWLGP IEVIR +TK IERE
Sbjct: 309 AAIMEHILDGSSYVKEALHLHKIDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIERE 368

Query: 415 INSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNN 474
           INSVNDNPLIDVSRNKA+HGGNFQGTPIGV+MDNTRLA+ASIGKLMFAQF+ELVNDFYNN
Sbjct: 369 INSVNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYNN 428

Query: 475 GLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSLGLIS 534
           GLPSNLS  RNPSLDYG KGAE+AMASYCSELQ+LANPVTNHV+SA QHNQDVNSLGLIS
Sbjct: 429 GLPSNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLIS 488

Query: 535 SRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSNGTLH 594
           SR TAEA+ ILKLMS+T+LVALCQA DLRHLEE LK  V   VS  AK VL       + 
Sbjct: 489 SRTTAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL------AIE 548

Query: 595 PSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNTSIFQ 654
           P R    D+L VV+REY F+YVDD  S T PLMQKLR VL + AL   + E +T   +F+
Sbjct: 549 PFR-KHDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKALAEPEGETDT---VFR 608

Query: 655 KISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLLTGEK 714
           KI AFE ELK +L KEVE  R  +E+G   + N+I++CRSYPLYRFVR EL T LLTGE 
Sbjct: 609 KIGAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTGED 668

Query: 715 VISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 751
           V SPGE+ +K+F  + QGK+ D + ECLKEWNGAPI IC
Sbjct: 669 VRSPGEDFDKVFRAISQGKLIDPLFECLKEWNGAPISIC 694

BLAST of MC01g_new0291 vs. TAIR 10
Match: AT5G04230.2 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 971.8 bits (2511), Expect = 3.0e-283
Identity = 508/703 (72.26%), Postives = 579/703 (82.36%), Query Frame = 0

Query: 55  SSIHDPLNWGMAAESLKGSHLDEVKRMVEEYRSPVVKLGGETLTISQVAAIATRDSDVVV 114
           +++ DPLNW +AAE+LKGSHL+EVK+MV++YR   V+LGGETLTI QVAA+A+      V
Sbjct: 9   TALSDPLNWNVAAEALKGSHLEEVKKMVKDYRKGTVQLGGETLTIGQVAAVAS--GGPTV 68

Query: 115 ELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGI 174
           EL+E AR GVKASSDWVMESMN+ TD+YG+TTGFG++S RRT QG ALQKELIR+LNAGI
Sbjct: 69  ELSEEARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGI 128

Query: 175 FGSGAES---SHTLPHSASRAAMLVRINTLLQGYSGIRFEILEAIASLLNHNVTPCLPLR 234
           F +G E    S+TLP  A+RAAML+R+NTLLQGYSGIRFEILEAI +LLN  +TP LPLR
Sbjct: 129 FATGNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLR 188

Query: 235 GTITASGDLVPLSYIAGLLTGRPNSKAIGSNGETLDAKEAFKQAGISSDFFELQPKEGLA 294
           GTITASGDLVPLSYIAG L GRPNS+++G +GE L A EAFK AG+SS FFEL+PKEGLA
Sbjct: 189 GTITASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVSS-FFELRPKEGLA 248

Query: 295 LVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEA 354
           LVNGTAVGS LAS VL++ANIL V SE+ SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEA
Sbjct: 249 LVNGTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEA 308

Query: 355 AAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDR----YALRTSPQWLGPLIEVIRFSTKS 414
           AAIMEHILDGSSY+K A  LH+IDPLQKPKQDR    YALRTSPQWLGP IEVIR +TK 
Sbjct: 309 AAIMEHILDGSSYVKEALHLHKIDPLQKPKQDRYVLGYALRTSPQWLGPQIEVIRAATKM 368

Query: 415 IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVND 474
           IEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGV+MDNTRLA+ASIGKLMFAQF+ELVND
Sbjct: 369 IEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVND 428

Query: 475 FYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTNHVQSAEQHNQDVNSL 534
           FYNNGLPSNLS  RNPSLDYG KGAE+AMASYCSELQ+LANPVTNHV+SA QHNQDVNSL
Sbjct: 429 FYNNGLPSNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSL 488

Query: 535 GLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTSSN 594
           GLISSR TAEA+ ILKLMS+T+LVALCQA DLRHLEE LK  V   VS  AK VL     
Sbjct: 489 GLISSRTTAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL----- 548

Query: 595 GTLHPSRFCEKDLLKVVDREYTFAYVDDACSATYPLMQKLRQVLVEHALTNGDDEKNTNT 654
             + P R    D+L VV+REY F+YVDD  S T PLMQKLR VL + AL   + E +T  
Sbjct: 549 -AIEPFR-KHDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKALAEPEGETDT-- 608

Query: 655 SIFQKISAFEEELKAILSKEVENARLAFESGNAKIKNQIEECRSYPLYRFVREELGTSLL 714
            +F+KI AFE ELK +L KEVE  R  +E+G   + N+I++CRSYPLYRFVR EL T LL
Sbjct: 609 -VFRKIGAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLL 668

Query: 715 TGEKVISPGEECEKIFTTLCQGKMTDSILECLKEWNGAPIPIC 751
           TGE V SPGE+ +K+F  + QGK+ D + ECLKEWNGAPI IC
Sbjct: 669 TGEDVRSPGEDFDKVFRAISQGKLIDPLFECLKEWNGAPISIC 698