MC01g_new0254 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0254
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Retrovirus-related Pol polyprotein from transposon RE1
Location	MC01: 10231222 .. 10232141 (+)
RNA-Seq Expression	MC01g_new0254
Synteny	MC01g_new0254

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGCCCCTACACCTATGGCAGTTGGACCCTCCCTCTCCATACATGATGGATCACCCCTTGAAAATGCCACAGAGTGGCATAGCTTAGTTGGAGCCTTGCAATATTGTACTGTCGCACGCCCAGATATCAATTTTGTTTTGTGGTCAACAAGTTGTGTCAATTCTTACATGCACCAACCACATCTCGTATGCAGGCTGTCAAGCGGCTTCTTCAGTACTTGAAGGGCTCTATTTCACTCAGGTATCTCCTTCACTTGAACTTCTTTTCATAATGTAACTTGCTATATTGATGTCGATTGGGCTAGTTGCCCAGATGACCGCAGCAGCACCAGTGGCTATTGTACCTTTATTGGTCCAATTTTAATTTCTTGGCACTCTGCAAAGCAAAAAGTAGTATCCAAGTCAAATGCCGAGTCTGAATATCGGGGTATGTCGAACGCAACTGCAGAAATTATATGGGTTCAATCGGTCCTCCATGAGCTTGCCTTATCACCTTCCTCTCCGCCTCTTCTCTTGTGTGACAATATCAGTGCCACATATCTTGTTGCCAACCCAGTGATGCACAATCGCACAAAGCATATTGAAATAGATCACCATTTTATACGGGAGCGTGTCGCTCGTCGACAACTCCTCATTCATTTTGTCCCTTCCGAAAATCAACTTGCTGATATAATGACAAAAGCATTGGCCTCTCCACGTTTTAGCAATCTTCGTACCAAGCTCACGGTTCTTCCCAAGTCCCGTTCACTTGCGGGGATGTTAAAATAAGTATTTTGTTAATTGTGTTTAGAGCTTAATTTATCCCTGTTTATAGTTTTTATGTTTTCCTCTTTTCTGTTATGGTCTCTCCAATAGTCATGTAACACGTGGATTACTTTTCTACTATTTAATAGTAGGGAAGTCACAGACCTCATTGAAGGC

mRNA sequence

ATGCAGGCTGTCAAGCGGCTTCTTCAGTACTTGAAGGGCTCTATTTCACTCAGTTGCCCAGATGACCGCAGCAGCACCAGTGGCTATTGTACCTTTATTGGTCCAATTTTAATTTCTTGGCACTCTGCAAAGCAAAAAGTAGTATCCAAGTCAAATGCCGAGTCTGAATATCGGGGTATGTCGAACGCAACTGCAGAAATTATATGGGTTCAATCGGTCCTCCATGAGCTTGCCTTATCACCTTCCTCTCCGCCTCTTCTCTTGTGTGACAATATCAGTGCCACATATCTTGTTGCCAACCCAGTGATGCACAATCGCACAAAGCATATTGAAATAGATCACCATTTTATACGGGAGCGTGTCGCTCGTCGACAACTCCTCATTCATTTTGTCCCTTCCGAAAATCAACTTGCTGATATAATGACAAAAGCATTGGCCTCTCCACGTTTTAGCAATCTTCGTACCAAGCTCACGGTTCTTCCCAAGTCCCGTTCACTTGCGGGGATGTTAAAATAA

Coding sequence (CDS)

ATGCAGGCTGTCAAGCGGCTTCTTCAGTACTTGAAGGGCTCTATTTCACTCAGTTGCCCAGATGACCGCAGCAGCACCAGTGGCTATTGTACCTTTATTGGTCCAATTTTAATTTCTTGGCACTCTGCAAAGCAAAAAGTAGTATCCAAGTCAAATGCCGAGTCTGAATATCGGGGTATGTCGAACGCAACTGCAGAAATTATATGGGTTCAATCGGTCCTCCATGAGCTTGCCTTATCACCTTCCTCTCCGCCTCTTCTCTTGTGTGACAATATCAGTGCCACATATCTTGTTGCCAACCCAGTGATGCACAATCGCACAAAGCATATTGAAATAGATCACCATTTTATACGGGAGCGTGTCGCTCGTCGACAACTCCTCATTCATTTTGTCCCTTCCGAAAATCAACTTGCTGATATAATGACAAAAGCATTGGCCTCTCCACGTTTTAGCAATCTTCGTACCAAGCTCACGGTTCTTCCCAAGTCCCGTTCACTTGCGGGGATGTTAAAATAA

Protein sequence

MQAVKRLLQYLKGSISLSCPDDRSSTSGYCTFIGPILISWHSAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANPVMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTVLPKSRSLAGMLK

Homology

[Close]

BLAST of MC01g_new0254 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.8e-31
Identity = 77/190 (40.53%), Postives = 112/190 (58.95%), Query Frame = 0


Query: 3    AVKRLLQYLKG------------SISLSC---------PDDRSSTSGYCTFIGPILISWH 62
            A+KR+L+YL G            ++SL            DD  ST+GY  ++G   ISW 
Sbjct: 1266 ALKRVLRYLAGTPDHGIFLKKGNTLSLHAYSDADWAGDTDDYVSTNGYIVYLGHHPISWS 1325

Query: 63   SAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANP 122
            S KQK V +S+ E+EYR ++N ++E+ W+ S+L EL +  S PP++ CDN+ ATYL ANP
Sbjct: 1326 SKKQKGVVRSSTEAEYRSVANTSSELQWICSLLTELGIQLSHPPVIYCDNVGATYLCANP 1385

Query: 123  VMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTVLP 172
            V H+R KHI +D+HFIR +V    L +  V + +QLAD +TK L+   F N   K+ V+ 
Sbjct: 1386 VFHSRMKHIALDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLSRVAFQNFSRKIGVIK 1445

[Close]

BLAST of MC01g_new0254 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.1e-31
Identity = 75/180 (41.67%), Postives = 109/180 (60.56%), Query Frame = 0


Query: 1    MQAVKRLLQYLKG------------SISLSC---------PDDRSSTSGYCTFIGPILIS 60
            +QA+KR+L+YL G            ++SL            DD  ST+GY  ++G   IS
Sbjct: 1281 LQALKRILRYLAGTPNHGIFLKKGNTLSLHAYSDADWAGDKDDYVSTNGYIVYLGHHPIS 1340

Query: 61   WHSAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVA 120
            W S KQK V +S+ E+EYR ++N ++E+ W+ S+L EL +  + PP++ CDN+ ATYL A
Sbjct: 1341 WSSKKQKGVVRSSTEAEYRSVANTSSEMQWICSLLTELGIRLTRPPVIYCDNVGATYLCA 1400

Query: 121  NPVMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTV 160
            NPV H+R KHI ID+HFIR +V    L +  V + +QLAD +TK L+   F N  +K+ V
Sbjct: 1401 NPVFHSRMKHIAIDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLSRTAFQNFASKIGV 1460

[Close]

BLAST of MC01g_new0254 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 105.1 bits (261), Expect = 7.8e-22
Identity = 64/190 (33.68%), Postives = 98/190 (51.58%), Query Frame = 0


Query: 2    QAVKRLLQYLKGSISL-----------------------SCPDDRSSTSGYCTFIGPI-L 61
            Q +KR+L+YLKG+I +                           DR ST+GY   +    L
Sbjct: 1219 QNLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEIDRKSTTGYLFKMFDFNL 1278

Query: 62   ISWHSAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYL 121
            I W++ +Q  V+ S+ E+EY  +  A  E +W++ +L  + +   +P  +  DN     +
Sbjct: 1279 ICWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTSINIKLENPIKIYEDNQGCISI 1338

Query: 122  VANPVMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKL 168
              NP  H R KHI+I +HF RE+V    + + ++P+ENQLADI TK L + RF  LR KL
Sbjct: 1339 ANNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQLADIFTKPLPAARFVELRDKL 1398

[Close]

BLAST of MC01g_new0254 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.7e-14
Identity = 55/169 (32.54%), Postives = 84/169 (49.70%), Query Frame = 0


Query: 2    QAVKRLLQYLKGS------ISLSCP--------------DDRSSTSGYCTFIGPILISWH 61
            +AVK +L+YL+G+         S P              D+R S++GY        ISW 
Sbjct: 1149 EAVKWILRYLRGTTGDCLCFGGSDPILKGYTDADMAGDIDNRKSSTGYLFTFSGGAISWQ 1208

Query: 62   SAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANP 121
            S  QK V+ S  E+EY   +    E+IW++  L EL L      ++ CD+ SA  L  N 
Sbjct: 1209 SKLQKCVALSTTEAEYIAATETGKEMIWLKRFLQELGLH-QKEYVVYCDSQSAIDLSKNS 1268

Query: 122  VMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRF 151
            + H RTKHI++ +H+IRE V    L +  + +    AD++TK +   +F
Sbjct: 1269 MYHARTKHIDVRYHWIREMVDDESLKVLKISTNENPADMLTKVVPRNKF 1316

[Close]

BLAST of MC01g_new0254 vs. ExPASy Swiss-Prot
Match: P92519 (Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 GN=AtMg00810 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-08
Identity = 30/87 (34.48%), Postives = 47/87 (54.02%), Query Frame = 0


Query: 4   VKRLLQYLKGSIS---------------------LSCPDDRSSTSGYCTFIGPILISWHS 63
           +KR+L+Y+KG+I                        C   R ST+G+CTF+G  +ISW +
Sbjct: 139 LKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCTFLGCNIISWSA 198

Query: 64  AKQKVVSKSNAESEYRGMSNATAEIIW 70
            +Q  VS+S+ E+EYR ++   AE+ W
Sbjct: 199 KRQPTVSRSSTETEYRALALTAAELTW 225

[Close]

BLAST of MC01g_new0254 vs. NCBI nr
Match: CAN68364.1 (hypothetical protein VITISV_021899 [Vitis vinifera])

HSP 1 Score: 201 bits (512), Expect = 1.07e-59
Identity = 101/192 (52.60%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 1   MQAVKRLLQYLKGSI----------SL-----------SCPDDRSSTSGYCTFIGPILIS 60
           +Q VKR+L+YLKGS+          SL           SCPDDR STSGYC F G  L+S
Sbjct: 138 LQVVKRVLRYLKGSLFYGLSFQPSSSLDLIAYTDADWASCPDDRRSTSGYCIFFGGNLVS 197

Query: 61  WHSAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVA 120
           W ++KQKVVS+S+ ESEYRG++NA AE+ W+QS+L EL +    PP+L CDN+S TYL A
Sbjct: 198 WSASKQKVVSRSSTESEYRGLANAAAELTWIQSLLKELFIPLFQPPVLYCDNLSTTYLAA 257

Query: 121 NPVMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTV 171
           NP++H+R KH+EID+HF+RERV ++ L + F+PSE+Q+ADI+TK+L++  F +LR+KLTV
Sbjct: 258 NPILHSRAKHVEIDYHFVRERVLQKTLDVRFLPSEDQVADILTKSLSTQHFLHLRSKLTV 317

[Close]

BLAST of MC01g_new0254 vs. NCBI nr
Match: RVW74919.1 (Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera])

HSP 1 Score: 195 bits (495), Expect = 8.77e-59
Identity = 103/187 (55.08%), Postives = 134/187 (71.66%), Query Frame = 0


Query: 3   AVKRLLQYLKGSISL---------------------SCPDDRSSTSGYCTFIGPILISWH 62
           AVKR+L+YLKG+ISL                     SCPDDR STSGYC F+G  LISW 
Sbjct: 69  AVKRILRYLKGTISLGLHFQTSTSMELQGYNDADWASCPDDRRSTSGYCVFLGSNLISWS 128

Query: 63  SAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANP 122
           S+KQ++VSK++A+SEYRG+ + TAE++W+QS+L EL L P+SPP+L CDN SA +L ANP
Sbjct: 129 SSKQRLVSKNSAKSEYRGLVSLTAELVWIQSLLQELCL-PTSPPVLWCDNQSAAHLAANP 188

Query: 123 VMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTVLP 168
           V H+R+KHIE+D HFIRE+V R++L I +V S +QLADI+TK L   +F +LR+KLTV  
Sbjct: 189 VFHSRSKHIELDLHFIREKVLRQELQICYVSSTDQLADILTKHLPISQFCSLRSKLTVTS 248

[Close]

BLAST of MC01g_new0254 vs. NCBI nr
Match: RVX00742.1 (Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera])

HSP 1 Score: 194 bits (494), Expect = 1.24e-57
Identity = 104/189 (55.03%), Postives = 132/189 (69.84%), Query Frame = 0


Query: 3   AVKRLLQYLKGSISL---------------------SCPDDRSSTSGYCTFIGPILISWH 62
           AVKR+L+YLKG+I L                     SC DDR STSGYC F+G  LISW 
Sbjct: 180 AVKRILRYLKGTIHLGLHFQPAASMELQGYSDANWASCLDDRRSTSGYCVFLGSNLISWS 239

Query: 63  SAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANP 122
           S+KQ++VSKS+ ESEYRG+ + T E++W+QS+L EL L P+SPP+L CDN SA +L ANP
Sbjct: 240 SSKQRLVSKSSVESEYRGLVSLTTELVWIQSLLQELCL-PTSPPILWCDNQSAAHLAANP 299

Query: 123 VMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTVLP 170
           V H+R+KHIE+D HFIRE+V R++L I +VPS +QLADI TK L   +F NLR+KLTV  
Sbjct: 300 VFHSRSKHIELDLHFIREKVLRQELQICYVPSGDQLADIFTKHLPITQFCNLRSKLTVTY 359

[Close]

BLAST of MC01g_new0254 vs. NCBI nr
Match: RVW25347.1 (Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera])

HSP 1 Score: 188 bits (477), Expect = 1.57e-57
Identity = 97/169 (57.40%), Postives = 125/169 (73.96%), Query Frame = 0


Query: 3   AVKRLLQYLKGSISL---------------------SCPDDRSSTSGYCTFIGPILISWH 62
           AVKR+L+YLKG+ISL                     SCPDDR STSGYC F+G  LISW 
Sbjct: 11  AVKRILRYLKGTISLGLHFQPSTSMELQGYSDADWASCPDDRRSTSGYCVFLGSNLISWS 70

Query: 63  SAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANP 122
           S+KQ++VSKS+AESEYRG+ + TAE++W+QS+L EL L P+SPP+L CDN SA +L ANP
Sbjct: 71  SSKQRLVSKSSAESEYRGLVSLTAELVWIQSLLQELCL-PTSPPVLWCDNQSAAHLAANP 130

Query: 123 VMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRF 150
           V H+R+KHIE+D HFIRE+V R++L I +VPS +QLADI+TK L+  +F
Sbjct: 131 VFHSRSKHIELDLHFIREKVLRQELQICYVPSTDQLADILTKHLSISQF 178

[Close]

BLAST of MC01g_new0254 vs. NCBI nr
Match: RVX14515.1 (Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera])

HSP 1 Score: 202 bits (513), Expect = 1.58e-56
Identity = 104/192 (54.17%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 1    MQAVKRLLQYLKGSI----------SL-----------SCPDDRSSTSGYCTFIGPILIS 60
            +QAVKR+L YLKGS+          SL           SCPDDR STSGYC F G  L+S
Sbjct: 866  LQAVKRVLCYLKGSLFYGLSFQPSSSLNLIAYTDADWASCPDDRRSTSGYCIFFGGNLVS 925

Query: 61   WHSAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVA 120
            W ++KQKVVS+S+ ESEYRG++NATAE+ W+QS+L EL +    PP+L CDN+S TYL A
Sbjct: 926  WSASKQKVVSRSSTESEYRGLANATAELTWIQSLLKELFVPLFQPPVLYCDNLSTTYLAA 985

Query: 121  NPVMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTV 171
            NPV+H+R KH+EID+HF+RERV ++ L + F+PSE+Q+ADI+ KAL++ RF +LR+KLTV
Sbjct: 986  NPVLHSRAKHVEIDYHFVRERVLQKTLDVRFLPSEDQVADILIKALSTQRFLHLRSKLTV 1045

[Close]

BLAST of MC01g_new0254 vs. ExPASy TrEMBL
Match: A5BVZ9 (Reverse transcriptase Ty1/copia-type domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_021899 PE=4 SV=1)

HSP 1 Score: 201 bits (512), Expect = 5.18e-60
Identity = 101/192 (52.60%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 1   MQAVKRLLQYLKGSI----------SL-----------SCPDDRSSTSGYCTFIGPILIS 60
           +Q VKR+L+YLKGS+          SL           SCPDDR STSGYC F G  L+S
Sbjct: 138 LQVVKRVLRYLKGSLFYGLSFQPSSSLDLIAYTDADWASCPDDRRSTSGYCIFFGGNLVS 197

Query: 61  WHSAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVA 120
           W ++KQKVVS+S+ ESEYRG++NA AE+ W+QS+L EL +    PP+L CDN+S TYL A
Sbjct: 198 WSASKQKVVSRSSTESEYRGLANAAAELTWIQSLLKELFIPLFQPPVLYCDNLSTTYLAA 257

Query: 121 NPVMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTV 171
           NP++H+R KH+EID+HF+RERV ++ L + F+PSE+Q+ADI+TK+L++  F +LR+KLTV
Sbjct: 258 NPILHSRAKHVEIDYHFVRERVLQKTLDVRFLPSEDQVADILTKSLSTQHFLHLRSKLTV 317

[Close]

BLAST of MC01g_new0254 vs. ExPASy TrEMBL
Match: A0A438GRU7 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Vitis vinifera OX=29760 GN=RE2_980 PE=4 SV=1)

HSP 1 Score: 195 bits (495), Expect = 4.25e-59
Identity = 103/187 (55.08%), Postives = 134/187 (71.66%), Query Frame = 0


Query: 3   AVKRLLQYLKGSISL---------------------SCPDDRSSTSGYCTFIGPILISWH 62
           AVKR+L+YLKG+ISL                     SCPDDR STSGYC F+G  LISW 
Sbjct: 69  AVKRILRYLKGTISLGLHFQTSTSMELQGYNDADWASCPDDRRSTSGYCVFLGSNLISWS 128

Query: 63  SAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANP 122
           S+KQ++VSK++A+SEYRG+ + TAE++W+QS+L EL L P+SPP+L CDN SA +L ANP
Sbjct: 129 SSKQRLVSKNSAKSEYRGLVSLTAELVWIQSLLQELCL-PTSPPVLWCDNQSAAHLAANP 188

Query: 123 VMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTVLP 168
           V H+R+KHIE+D HFIRE+V R++L I +V S +QLADI+TK L   +F +LR+KLTV  
Sbjct: 189 VFHSRSKHIELDLHFIREKVLRQELQICYVSSTDQLADILTKHLPISQFCSLRSKLTVTS 248

[Close]

BLAST of MC01g_new0254 vs. ExPASy TrEMBL
Match: A0A438IVH0 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Vitis vinifera OX=29760 GN=RE2_267 PE=4 SV=1)

HSP 1 Score: 194 bits (494), Expect = 6.02e-58
Identity = 104/189 (55.03%), Postives = 132/189 (69.84%), Query Frame = 0


Query: 3   AVKRLLQYLKGSISL---------------------SCPDDRSSTSGYCTFIGPILISWH 62
           AVKR+L+YLKG+I L                     SC DDR STSGYC F+G  LISW 
Sbjct: 180 AVKRILRYLKGTIHLGLHFQPAASMELQGYSDANWASCLDDRRSTSGYCVFLGSNLISWS 239

Query: 63  SAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANP 122
           S+KQ++VSKS+ ESEYRG+ + T E++W+QS+L EL L P+SPP+L CDN SA +L ANP
Sbjct: 240 SSKQRLVSKSSVESEYRGLVSLTTELVWIQSLLQELCL-PTSPPILWCDNQSAAHLAANP 299

Query: 123 VMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTVLP 170
           V H+R+KHIE+D HFIRE+V R++L I +VPS +QLADI TK L   +F NLR+KLTV  
Sbjct: 300 VFHSRSKHIELDLHFIREKVLRQELQICYVPSGDQLADIFTKHLPITQFCNLRSKLTVTY 359

[Close]

BLAST of MC01g_new0254 vs. ExPASy TrEMBL
Match: A0A438CQ69 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Vitis vinifera OX=29760 GN=RE2_739 PE=4 SV=1)

HSP 1 Score: 188 bits (477), Expect = 7.59e-58
Identity = 97/169 (57.40%), Postives = 125/169 (73.96%), Query Frame = 0


Query: 3   AVKRLLQYLKGSISL---------------------SCPDDRSSTSGYCTFIGPILISWH 62
           AVKR+L+YLKG+ISL                     SCPDDR STSGYC F+G  LISW 
Sbjct: 11  AVKRILRYLKGTISLGLHFQPSTSMELQGYSDADWASCPDDRRSTSGYCVFLGSNLISWS 70

Query: 63  SAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVANP 122
           S+KQ++VSKS+AESEYRG+ + TAE++W+QS+L EL L P+SPP+L CDN SA +L ANP
Sbjct: 71  SSKQRLVSKSSAESEYRGLVSLTAELVWIQSLLQELCL-PTSPPVLWCDNQSAAHLAANP 130

Query: 123 VMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRF 150
           V H+R+KHIE+D HFIRE+V R++L I +VPS +QLADI+TK L+  +F
Sbjct: 131 VFHSRSKHIELDLHFIREKVLRQELQICYVPSTDQLADILTKHLSISQF 178

[Close]

BLAST of MC01g_new0254 vs. ExPASy TrEMBL
Match: A0A438JZY3 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=29760 GN=RE1_1099 PE=4 SV=1)

HSP 1 Score: 202 bits (513), Expect = 7.65e-57
Identity = 104/192 (54.17%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 1    MQAVKRLLQYLKGSI----------SL-----------SCPDDRSSTSGYCTFIGPILIS 60
            +QAVKR+L YLKGS+          SL           SCPDDR STSGYC F G  L+S
Sbjct: 866  LQAVKRVLCYLKGSLFYGLSFQPSSSLNLIAYTDADWASCPDDRRSTSGYCIFFGGNLVS 925

Query: 61   WHSAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVA 120
            W ++KQKVVS+S+ ESEYRG++NATAE+ W+QS+L EL +    PP+L CDN+S TYL A
Sbjct: 926  WSASKQKVVSRSSTESEYRGLANATAELTWIQSLLKELFVPLFQPPVLYCDNLSTTYLAA 985

Query: 121  NPVMHNRTKHIEIDHHFIRERVARRQLLIHFVPSENQLADIMTKALASPRFSNLRTKLTV 171
            NPV+H+R KH+EID+HF+RERV ++ L + F+PSE+Q+ADI+ KAL++ RF +LR+KLTV
Sbjct: 986  NPVLHSRAKHVEIDYHFVRERVLQKTLDVRFLPSEDQVADILIKALSTQRFLHLRSKLTV 1045

[Close]

BLAST of MC01g_new0254 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 124.0 bits (310), Expect = 1.1e-28
Identity = 65/140 (46.43%), Postives = 83/140 (59.29%), Query Frame = 0


Query: 2   QAVKRLLQYLKGSIS---------------------LSCPDDRSSTSGYCTFIGPILISW 61
           QAV ++L Y+KG++                       SC D R ST+GYC F+G  LISW
Sbjct: 415 QAVMKILHYIKGTVGQGLFYSSQAEMQLQVFSDASFQSCKDTRRSTNGYCMFLGTSLISW 474

Query: 62  HSAKQKVVSKSNAESEYRGMSNATAEIIWVQSVLHELALSPSSPPLLLCDNISATYLVAN 121
            S KQ+VVSKS+AE+EYR +S AT E++W+     EL L  S P LL CDN +A ++  N
Sbjct: 475 KSKKQQVVSKSSAEAEYRALSFATDEMMWLAQFFRELQLPLSKPTLLFCDNTAAIHIATN 534

[Close]

BLAST of MC01g_new0254 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.0e-09
Identity = 30/87 (34.48%), Postives = 47/87 (54.02%), Query Frame = 0


Query: 4   VKRLLQYLKGSIS---------------------LSCPDDRSSTSGYCTFIGPILISWHS 63
           +KR+L+Y+KG+I                        C   R ST+G+CTF+G  +ISW +
Sbjct: 139 LKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCTFLGCNIISWSA 198

Query: 64  AKQKVVSKSNAESEYRGMSNATAEIIW 70
            +Q  VS+S+ E+EYR ++   AE+ W
Sbjct: 199 KRQPTVSRSSTETEYRALALTAAELTW 225

The following BLAST results are available for this feature:

BLAST of MC01g_new0254 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZT94	1.8e-31	40.53	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]
Q94HW2	3.1e-31	41.67	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
P04146	7.8e-22	33.68	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]
P10978	2.7e-14	32.54	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P92519	2.9e-08	34.48	Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of MC01g_new0254 vs. NCBI nr


Match Name	E-value	Identity	Description
CAN68364.1	1.07e-59	52.60	hypothetical protein VITISV_021899 [Vitis vinifera]	[more]
RVW74919.1	8.77e-59	55.08	Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera]	[more]
RVX00742.1	1.24e-57	55.03	Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera]	[more]
RVW25347.1	1.57e-57	57.40	Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera]	[more]
RVX14515.1	1.58e-56	54.17	Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera]	[more]

BLAST of MC01g_new0254 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A5BVZ9	5.18e-60	52.60	Reverse transcriptase Ty1/copia-type domain-containing protein OS=Vitis vinifera...	[more]
A0A438GRU7	4.25e-59	55.08	Retrovirus-related Pol polyprotein from transposon RE2 OS=Vitis vinifera OX=2976...	[more]
A0A438IVH0	6.02e-58	55.03	Retrovirus-related Pol polyprotein from transposon RE2 OS=Vitis vinifera OX=2976...	[more]
A0A438CQ69	7.59e-58	57.40	Retrovirus-related Pol polyprotein from transposon RE2 OS=Vitis vinifera OX=2976...	[more]
A0A438JZY3	7.65e-57	54.17	Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=2976...	[more]

BLAST of MC01g_new0254 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23160.1	1.1e-28	46.43	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]
ATMG00810.1	2.0e-09	34.48	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 40..160
None	No IPR available	PANTHER	PTHR11439:SF343	SUBFAMILY NOT NAMED	coord: 40..160
None	No IPR available	CDD	cd09272	RNase_HI_RT_Ty1	coord: 18..148 e-value: 2.57344E-60 score: 181.898

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g_new0254.1	MC01g_new0254.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043170	macromolecule metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0044238	primary metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0005488	binding