MC01g_new0248 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0248
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	CCHC-type domain-containing protein
Location	MC01: 9896104 .. 9900648 (-)
RNA-Seq Expression	MC01g_new0248
Synteny	MC01g_new0248

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATAATGCCCCCACCAAAAACCCTCAACTATCCTCAATCGTAGGTCCTAATTTTCCCCCTTCTTCTCCTTCTTCAACGAGCAAGAAGCAGAGCCTCCCATGGCCGCCGCCGCCACCGCCGCCACCGCCGCCGGCGCGGAAACTCCATAGATTCACCGTATCTCCCTCCTTCAACTCGTTCCTCTCCACCAGGCCGTGCCACTCCGTGATCATCACATACGCCGGCCCCTTCCTCGTCTCCCACTTGCTCAGCGTCATCAAACTCACAGAAGAGTCCGGCTCGATGATCTCCACCATCATCCACTTCTCCTGGTTCAATTTTTCCTCCTCCTCCAGTTCCATAAAGTTCTCTCTAATCTTCCTCTGCGGAATCACCAGCCGATTGCATCTCTCGTCCACATCCGCTTTCCCCAATTTCTTCGCCATCACGAAATTAGGGCCCGAACAACATCCTCCCATGAAATTCATCGCTCTCTGGATCTCCTGCGGCAGGTCGTAGCTCACCGCTCCCCGGACTTCCGCTAATTGCGGCATCGCCTCGATGATTAGGGCGCATTCTCGATCGGTGATGACCTCGCCCTCGCCCTCGCTCTCGCTCTCGCACTCCGCGGCCGGTGGTGCCGCTTCGAGACTTTGAACTTCTTTCGATTCGACGATTGGGCGTTTCAGAAGCTTTTCTCTCAATAATCTCAGTTGCTTCAGGTCTGCTTCCATATCCCTCCTCTTCCTCTTCTTCCTCTTTTTTAGTGGCATTCGTTCATTGTCGTCGTTAGTGAGATCGATTATTGTGATGGTTTCTTCTTCTGCAACCTCTTTCTTTTCTTGATTTTCGTCGACTTCGCCGGTTGTTAGTGGAATTTCTTTCTTGTCGTCGTTTGTGAGGTTGAATTGACTTTGACTGGTGGTTGCTTCTTCTGCAACCTCTCTGTTTTCTTGACGTTCACCATCCTCACCGGTTTTTAGTGGAATTTCTTTCTCGTCGTCGCTAATGAGAACGAATTGAGTGGCGGTTTCTTGTTCTGCAACCTCCTTGTTTTCTTGATGTTCATCGTCTTTGCCTGTTTCTACTGGAATTTGTTTCTCGTCTTCGTTACTGAGATTGAGTACTTCAGTGGTGGTTTCTTTTTCTGGAACCTCTTCGTTGTCTTGATGTTCATCGTCTTCGCCGGCGTGATCTGAACGGTGTGGATGCATAATCTCGCCGGCGACGAAGAAGAAGAAGAAAAAGAAAATAACGGATGTGGTTTTTGGGAGGATTAAATCTAATGGGCAGCATTTTTTTCCTTAAAAAAAGGTTAAAATATTCATTTTTTTGGACGAAAAATACACTGTTTAGTTTAAATTTTAGTTCACCTTAAAGAGTCTATCATTTTTGTCCATAATTACAAAGTTTCAACACAAATTCTACCTACAATACAATATATTTTAATACAAATTTGTGGTTATATTTTAAATATAATGTATTGAGTATAATGTAAAAAGATCAAAATAATATTTTAATAAAAAATTTTGGTTTAAATTCTATTTCAACAATTTAAATTTTGAAAGGTTTATTTTACTCTAGACTTAGAAAATAACTATTTAAAATATCAATTTTACTTAATTTTAAAACATAACTATTTTGATTATTGTTATTATTTTATTACACAATATTTTCACATCTAATATTCCAGTCTACATGATTATAATATTAATCTACATTGTCTACGATATTAACATAGGTAGTTTAGGAGATAATACATCAACCAACGATAAACTATTTGATTGCAAGAATTTAATATTGCAATCAAATAGTATTGTTAGTACTAGTTTGATTTTATCACTTTGAATGTTTTTTTTATGGGTTTGTGTATTTATTTTTTGTATTTTAGATTTTGTTACCTATGTTGATTTTGTGCTGATTTAGATATGAGTTTGGATATTTTGTTGTTGAGTTTGAGATTTTTTACATGTTTGATAATTTTTTTTATTGAGTTTAAACCATTTATATGAGTTGATATTGAGGTCTTTGTTGGTTGAATTCTATAGGGTCAAGACTAATTATTGAGTATTTTTCTAATATAGAATTCTAATTTTATAAATTTTATCCATTATTAAAATTCAGGGTTTGAATTCAATAAAGTAAACAAAATTTGAGGTTAAAATTGATAAAAAAAAAAATTTGCTGAAAATTTAACCTTTTTTTTCAGCAATATTTGATAATTTTCAATTATGTACCTATTTGTGTGAACTGTGGTTCTATTCCAATTTGGAAGTGAAAAGAAAATAAATAAATAAAACCCTTCGTGGGTAGACTAAAAGAAACCCATTTGAAAAAGAAAAAAAAAATCATTAACTATTCCATTCAAAAGACATGGGTGATTTGCCAATTTATATTAATGAATAATGATTTTTGAAGGATAATATTGGAATCTTACTCGAAAAAGAAAGATTAATAAATCGGACTTTATCAATAATTTAGCCCTTAACTTTTTTTTCGTACTCCTCTAGGATGTACGCTCTCTCTCTCAGTCGGTGACTGAGGCTTTTCGGGAGCTCGCCTTTCTTCGTTTTCTTTCCTGCGAGAGGGGAGGCCTTTCCACTCTCAATATGGACCACGAAAATCTTCTAGCAGATTGGCAGAAATTCAAGCTGACATCGGAAGAGGACGAGATTGCGATGGATGTGGATGTTGATGCTGTAAAGATGGCTGAGCAGGGGTTAGCGTATAGCTTGGTGGGCAAACTCCTCGCCAAAAGGATTATCAGCGCTGATGTGTTATCCAGGGTGCTCTTGTTAGCTTGGAAGGTGGAACACCAATTAACGGTGGAGAGCATTGGGAAAAACTTGTTCCTGTTCCATTTTTGCAGGGAATGTGATATGAATAGAGTAATGAAAACAGGTCCTTGGTTTTTCGATAAGGCTCTCATTGTTCTTCAAAAACCATGTTCCTCAAAAAATATTTCCGAATTGGAGTTCAATAGGGTGGCTTTCTGGATTCATTTATTTGATCTTCCAATGTCATGGTTGAATAAGACAATGGCAATCCGTCTAGGGAATGCGATTGGAAACTTTGTGGATGTTGATTGCAATGAGAAAGGATTCAGTTGGGGTGCAAGCCTGCGAATTAGGGTATTGATTGACATAACCAAACCACTGAGAAGAGGAATTAAGATTAACATTGATGGTCCAATGGGAGGATGTTGGATTCCGATCCAATATGAACGCTTGCCCGACTTTTGCTATTTTTGTGGTGTGATAGGTCATTCTTCACATGATTGTGATGCACGCTATCTAGCTGCACAGGATGATAGCAGAGCCACCAGTGAGTACGGGCCATGGCTGCGGTTTGTTGGTTCAAAAGCTGGGGCTCAGAAGGGGAGGAAAGGCAAATCACCAGCTCGCGAGGATTCTTGCGGATCAAGCTCTATGAATTCGAAGGAGCGGGGTGTGGAGGAGACAAAGCAACAGTTGAGTGAGCAGACAAACCAGGATGGTTTCCAAAGTCAGGCAGCGGCGGAACAAACTGGGGATGCCGGCGGCCGAGCGTCGGCCACGGGATGTGCTCTTGGAATGGAAAGTAACAGTTGGGATCACTCCATTAATGATTTTCCTAAATCATCCCAAGGATCAAGGAAAAAATTAATGAGTGATGTGCTGAATAAAAGGAAACCGAAGGATTTGATGAATCAGAATTCAAGTGGAGCTAGATTCCAGGATTTACAGAAAGAAGTTACAAATATTCCTTTTTTCAAATGCACATGGTGATATGGGGATTTCCCTTTCTGATGATAAGAATGGGTCAGGGAGCAATTCTGAAAATAAAAAGCCCATTAATATTGTGAACTCTGGCCCAGAATCTTCCAATCCCACTTCAGTAAAAAATTGGAAGAGGATGGCCCGAAGCAAGAAAATCAATGTAAGTTCGCAAGTGATACAGATTCACACTGGCCACAAACGAGAGTGTCAGACCCTGATGGCTGAAGAACCGAATAAGAAGGGGAAACTAATTGCGGCAGATTTGGATTGCCTTTCAGATGTCCTGTTTCAAACGGCAGAGGCTGCTTGTCAGCCCTGCCGAGCACCATGAAAATTCTTTGTTGGAATGTCCGGGGGTTGGGGAACCCGAGGACATTCCGTGATGTATGTGACTATTTAAGTCATCTTAATCCCCTTATTTTATTTTTGTCTGAAACTAAATGTAATTCCAGTATCTTGAATAATTTAAAAGTTCGTTTGAATTATGCGGGTTGTTTTGTGGTTGATAGCAGTGCCAGAAGTGGCGGTTTATGTTTGCTTTGGAGCCAGGAGGTGAACATTTCAATCCGATCTTTCTCAAGGTTCCATATCGATGCATGGGTGGTGTGGCAGACAAAATCTTTTCAGTTCTCGGGAGTCTATGGTGATCCAAAGGCTCATACGATTTCTCACATGGGACCTCATTCAGAGACTTAATACGCGGTCTAATAACGCCTGGCTGGTAGGGGGAGATCTCAATGAGATATTGTATGATAGTGAGAAGTGTGGAGGTTCTGACAGAAACTATTCAGCAATTGAAAGATTCAG

mRNA sequence

ATGGACCACGAAAATCTTCTAGCAGATTGGCAGAAATTCAAGCTGACATCGGAAGAGGACGAGATTGCGATGGATGTGGATGTTGATGCTGTAAAGATGGCTGAGCAGGGGTTAGCGTATAGCTTGGTGGGCAAACTCCTCGCCAAAAGGATTATCAGCGCTGATGTGTTATCCAGGGTGCTCTTGTTAGCTTGGAAGGTGGAACACCAATTAACGGTGGAGAGCATTGGGAAAAACTTGTTCCTGTTCCATTTTTGCAGGGAATGTGATATGAATAGAGTAATGAAAACAGGTCCTTGGTTTTTCGATAAGGCTCTCATTGTTCTTCAAAAACCATGTTCCTCAAAAAATATTTCCGAATTGGAGTTCAATAGGGTGGCTTTCTGGATTCATTTATTTGATCTTCCAATGTCATGGTTGAATAAGACAATGGCAATCCGTCTAGGGAATGCGATTGGAAACTTTGTGGATGTTGATTGCAATGAGAAAGGATTCAGTTGGGGTGCAAGCCTGCGAATTAGGGTATTGATTGACATAACCAAACCACTGAGAAGAGGAATTAAGATTAACATTGATGGTCCAATGGGAGGATGTTGGATTCCGATCCAATATGAACGCTTGCCCGACTTTTGCTATTTTTGTGGTGTGATAGGTCATTCTTCACATGATTGTGATGCACGCTATCTAGCTGCACAGGATGATAGCAGAGCCACCAGTGAGTACGGGCCATGGCTGCGGTTTGTTGGTTCAAAAGCTGGGGCTCAGAAGGGGAGGAAAGGCAAATCACCAGCTCGCGAGGATTCTTGCGGATCAAGCTCTATGAATTCGAAGGAGCGGGGTGTGGAGGAGACAAAGCAACAGTTGAGTGAGCAGACAAACCAGGATGGTTTCCAAAGTCAGGCAGCGGCGGAACAAACTGGGGATGCCGGCGGCCGAGCGTCGGCCACGGGATGTGCTCTTGGAATGGAAAGTAACAGTTGGGATCACTCCATTAATGATTTTCCTAAATCATCCCAAGGATCAAGGAAAAAATTAATGAGTGATGTGCTGAATAAAAGGAAACCGAAGGATTTGATGAATCAGAATTCAAGTGGAGCTAGATTCCAGGATTTACAGAAAGAAGTTACAAATATTCCTTTTTTCAAATGCACATGGTGA

Coding sequence (CDS)

ATGGACCACGAAAATCTTCTAGCAGATTGGCAGAAATTCAAGCTGACATCGGAAGAGGACGAGATTGCGATGGATGTGGATGTTGATGCTGTAAAGATGGCTGAGCAGGGGTTAGCGTATAGCTTGGTGGGCAAACTCCTCGCCAAAAGGATTATCAGCGCTGATGTGTTATCCAGGGTGCTCTTGTTAGCTTGGAAGGTGGAACACCAATTAACGGTGGAGAGCATTGGGAAAAACTTGTTCCTGTTCCATTTTTGCAGGGAATGTGATATGAATAGAGTAATGAAAACAGGTCCTTGGTTTTTCGATAAGGCTCTCATTGTTCTTCAAAAACCATGTTCCTCAAAAAATATTTCCGAATTGGAGTTCAATAGGGTGGCTTTCTGGATTCATTTATTTGATCTTCCAATGTCATGGTTGAATAAGACAATGGCAATCCGTCTAGGGAATGCGATTGGAAACTTTGTGGATGTTGATTGCAATGAGAAAGGATTCAGTTGGGGTGCAAGCCTGCGAATTAGGGTATTGATTGACATAACCAAACCACTGAGAAGAGGAATTAAGATTAACATTGATGGTCCAATGGGAGGATGTTGGATTCCGATCCAATATGAACGCTTGCCCGACTTTTGCTATTTTTGTGGTGTGATAGGTCATTCTTCACATGATTGTGATGCACGCTATCTAGCTGCACAGGATGATAGCAGAGCCACCAGTGAGTACGGGCCATGGCTGCGGTTTGTTGGTTCAAAAGCTGGGGCTCAGAAGGGGAGGAAAGGCAAATCACCAGCTCGCGAGGATTCTTGCGGATCAAGCTCTATGAATTCGAAGGAGCGGGGTGTGGAGGAGACAAAGCAACAGTTGAGTGAGCAGACAAACCAGGATGGTTTCCAAAGTCAGGCAGCGGCGGAACAAACTGGGGATGCCGGCGGCCGAGCGTCGGCCACGGGATGTGCTCTTGGAATGGAAAGTAACAGTTGGGATCACTCCATTAATGATTTTCCTAAATCATCCCAAGGATCAAGGAAAAAATTAATGAGTGATGTGCTGAATAAAAGGAAACCGAAGGATTTGATGAATCAGAATTCAAGTGGAGCTAGATTCCAGGATTTACAGAAAGAAGTTACAAATATTCCTTTTTTCAAATGCACATGGTGA

Protein sequence

MDHENLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLLLAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEFNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPLRRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSEYGPWLRFVGSKAGAQKGRKGKSPAREDSCGSSSMNSKERGVEETKQQLSEQTNQDGFQSQAAAEQTGDAGGRASATGCALGMESNSWDHSINDFPKSSQGSRKKLMSDVLNKRKPKDLMNQNSSGARFQDLQKEVTNIPFFKCTW

Homology

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BLAST of MC01g_new0248 vs. NCBI nr
Match: XP_022156185.1 (uncharacterized protein LOC111023135 [Momordica charantia] >XP_022156186.1 uncharacterized protein LOC111023135 [Momordica charantia])

HSP 1 Score: 778 bits (2008), Expect = 8.70e-284
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0


Query: 1   MDHENLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRV 60
           MDHENLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRV
Sbjct: 1   MDHENLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRV 60

Query: 61  LLLAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISE 120
           LLLAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISE
Sbjct: 61  LLLAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISE 120

Query: 121 LEFNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDIT 180
           LEFNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDIT
Sbjct: 121 LEFNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDIT 180

Query: 181 KPLRRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSE 240
           KPLRRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSE
Sbjct: 181 KPLRRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSE 240

Query: 241 YGPWLRFVGSKAGAQKGRKGKSPAREDSCGSSSMNSKERGVEETKQQLSEQTNQDGFQSQ 300
           YGPWLRFVGSKAGAQKGRKGKSPAREDSCGSSSMNSKERGVEETKQQLSEQTNQDGFQSQ
Sbjct: 241 YGPWLRFVGSKAGAQKGRKGKSPAREDSCGSSSMNSKERGVEETKQQLSEQTNQDGFQSQ 300

Query: 301 AAAEQTGDAGGRASATGCALGMESNSWDHSINDFPKSSQGSRKKLMSDVLNKRKPKDLMN 360
           AAAEQTGDAGGRASATGCALGMESNSWDHSINDFPKSSQGSRKKLMSDVLNKRKPKDLMN
Sbjct: 301 AAAEQTGDAGGRASATGCALGMESNSWDHSINDFPKSSQGSRKKLMSDVLNKRKPKDLMN 360

Query: 361 QNSSGARFQDLQKEVTNIPFFKCTW 385
           QNSSGARFQDLQKEVTNIPFFKCTW
Sbjct: 361 QNSSGARFQDLQKEVTNIPFFKCTW 385

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BLAST of MC01g_new0248 vs. NCBI nr
Match: XP_022132681.1 (uncharacterized protein LOC111005481 [Momordica charantia])

HSP 1 Score: 271 bits (692), Expect = 1.00e-85
Identity = 129/248 (52.02%), Postives = 169/248 (68.15%), Query Frame = 0


Query: 5   NLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLLLA 64
           NLL +W+ FKLTSEED+IA+D+D  A++   + L  SL+ KLL+KR IS  VL   L +A
Sbjct: 5   NLLEEWKNFKLTSEEDKIAVDIDSSALEGTGKCLELSLICKLLSKRSISCTVLKNTLKIA 64

Query: 65  WKVEHQ-LTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEF 124
           WK++ +  +V+ IG N+FLF+F R  D NR+++ GPW FD+ALI++  P S     +++F
Sbjct: 65  WKLDCKAFSVDIIGFNIFLFNFNRSSDRNRILRMGPWTFDRALIIIDNPVSLTKPLDMDF 124

Query: 125 NRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPL 184
             V+ W+H FDL ++ +NKTMA RLGNAIG F DV+ N   F WG+ LR+RV  D+ KPL
Sbjct: 125 RNVSLWVHFFDLSLACMNKTMATRLGNAIGLFEDVESNANNFCWGSCLRVRVRFDVMKPL 184

Query: 185 RRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSEYGP 244
            RGIK+N+DGPMGGCWIPIQYERLPDF Y CG + H   DC        D      +YGP
Sbjct: 185 HRGIKLNLDGPMGGCWIPIQYERLPDFRYHCGRLDHILKDCSD---CCVDSVSKNLQYGP 244

Query: 245 WLRFVGSK 251
           WLRF G K
Sbjct: 245 WLRFQGHK 249

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BLAST of MC01g_new0248 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 252 bits (644), Expect = 9.65e-71
Identity = 118/247 (47.77%), Postives = 162/247 (65.59%), Query Frame = 0


Query: 5   NLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLLLA 64
           +LL +W+ FKLTSEE+E A+DVD  A       L   LVGKL  KR I+  V+   +  A
Sbjct: 5   DLLEEWKNFKLTSEEEETAIDVDASAPATTGSRLEQILVGKLFIKRPITCPVMKNTMRTA 64

Query: 65  WKVEHQ-LTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEF 124
           WK+E+    V+S+G NLFLF F R  D N++ K+GPW FD+ L+++ KP +    SEL+F
Sbjct: 65  WKLENNAFEVQSLGYNLFLFSFARALDRNKIYKSGPWTFDRTLVLINKPVALIPPSELDF 124

Query: 125 NRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPL 184
            ++  W+  FDLP+  + + MAIRLGNA+G F + DC++    WG++LR+RV++DI+KPL
Sbjct: 125 TKLPIWVRFFDLPLGCITRDMAIRLGNALGGFEEADCDDLNPDWGSNLRVRVMLDISKPL 184

Query: 185 RRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSEYGP 244
           RRGIK+N+DGP+GG WIPIQYERLPDFCY CG+                  SR   +YG 
Sbjct: 185 RRGIKLNLDGPIGGAWIPIQYERLPDFCYHCGL----------------SSSRKKHQYGS 235

Query: 245 WLRFVGS 250
           WLR+ G+
Sbjct: 245 WLRYQGT 235

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BLAST of MC01g_new0248 vs. NCBI nr
Match: XP_028117212.1 (uncharacterized protein LOC114314884 [Camellia sinensis])

HSP 1 Score: 190 bits (483), Expect = 2.05e-54
Identity = 92/230 (40.00%), Postives = 132/230 (57.39%), Query Frame = 0


Query: 17  SEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLLLAWKVEHQLTVESI 76
           +EE+E  + VD   +   E      LVGKLL  R  + D L   L   WK    +  ++ 
Sbjct: 15  TEEEEAEVVVDEVHIHSTEANAGLCLVGKLLTMRPFNMDALRSTLTSVWKASKGVVFKTF 74

Query: 77  GKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEFNRVAFWIHLFDLP 136
           G NL L  F    D  RVM  GPW FDK L++L++   +   SE+ F+ + FW+H  +LP
Sbjct: 75  GDNLVLIQFGHIVDKRRVMINGPWSFDKQLVMLKEFNRALQPSEITFSTMEFWVHASNLP 134

Query: 137 MSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPLRRGIKINIDGPMG 196
           +  + + + + LGN +G FVD++  + G +WG +L IR+ I+I KPLRRG+K+ + G   
Sbjct: 135 LISMTRDVGVLLGNTLGKFVDMEYGDGGIAWGRALHIRIEINIDKPLRRGLKLALGG-QE 194

Query: 197 GCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSEYGPWLR 246
             WI +QYERLP+FC+ CG++GHS HDC  R L          +YGPWLR
Sbjct: 195 PVWITLQYERLPNFCFHCGLLGHSVHDCGNRLLEGAHREEVRLQYGPWLR 243

[Close]

BLAST of MC01g_new0248 vs. NCBI nr
Match: XP_028122006.1 (uncharacterized protein LOC114319195 [Camellia sinensis])

HSP 1 Score: 188 bits (477), Expect = 2.37e-51
Identity = 97/251 (38.65%), Postives = 150/251 (59.76%), Query Frame = 0


Query: 4   ENLLADWQKFKLTSEEDEIA-MDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLL 63
           ++LL   +   LTSEED +  +  +  ++ M +  +   LVGKLL +R  + + +   LL
Sbjct: 3   DSLLDLTRTLSLTSEEDAVVRIGGESTSLMMGKSDMC--LVGKLLTRRPFNVEAMKNTLL 62

Query: 64  LAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELE 123
             W+    + V  IG NLF+F F    D  RV+  GPW FDK L++L +   +   S+++
Sbjct: 63  SVWQPTKGMQVRVIGDNLFVFVFGHVVDKRRVLSDGPWTFDKHLLMLGEMDPNVQPSDIQ 122

Query: 124 FNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKP 183
             RV FW+H+ +LP+  +NK +   +GNA+G F+D+D  + G  WG ++RIRV +D+ KP
Sbjct: 123 LTRVQFWVHVCNLPLVLMNKKVGEIVGNAVGQFIDMDYEDGGIVWGRTMRIRVALDVRKP 182

Query: 184 LRRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATS-EY 243
           LRRG+K+ +       W+  +YERLP +CYFCG +GHS  +CD + L++ D SR  S +Y
Sbjct: 183 LRRGMKLALSSAEP-IWVDFKYERLPIYCYFCGRLGHSERECDDK-LSSADGSRVDSLQY 242

Query: 244 GPWLRFVGSKA 252
           G WLR    K+
Sbjct: 243 GAWLRMDNIKS 249

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BLAST of MC01g_new0248 vs. ExPASy TrEMBL
Match: A0A6J1DU55 (uncharacterized protein LOC111023135 OS=Momordica charantia OX=3673 GN=LOC111023135 PE=4 SV=1)

HSP 1 Score: 778 bits (2008), Expect = 4.21e-284
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0


Query: 1   MDHENLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRV 60
           MDHENLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRV
Sbjct: 1   MDHENLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRV 60

Query: 61  LLLAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISE 120
           LLLAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISE
Sbjct: 61  LLLAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISE 120

Query: 121 LEFNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDIT 180
           LEFNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDIT
Sbjct: 121 LEFNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDIT 180

Query: 181 KPLRRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSE 240
           KPLRRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSE
Sbjct: 181 KPLRRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSE 240

Query: 241 YGPWLRFVGSKAGAQKGRKGKSPAREDSCGSSSMNSKERGVEETKQQLSEQTNQDGFQSQ 300
           YGPWLRFVGSKAGAQKGRKGKSPAREDSCGSSSMNSKERGVEETKQQLSEQTNQDGFQSQ
Sbjct: 241 YGPWLRFVGSKAGAQKGRKGKSPAREDSCGSSSMNSKERGVEETKQQLSEQTNQDGFQSQ 300

Query: 301 AAAEQTGDAGGRASATGCALGMESNSWDHSINDFPKSSQGSRKKLMSDVLNKRKPKDLMN 360
           AAAEQTGDAGGRASATGCALGMESNSWDHSINDFPKSSQGSRKKLMSDVLNKRKPKDLMN
Sbjct: 301 AAAEQTGDAGGRASATGCALGMESNSWDHSINDFPKSSQGSRKKLMSDVLNKRKPKDLMN 360

Query: 361 QNSSGARFQDLQKEVTNIPFFKCTW 385
           QNSSGARFQDLQKEVTNIPFFKCTW
Sbjct: 361 QNSSGARFQDLQKEVTNIPFFKCTW 385

[Close]

BLAST of MC01g_new0248 vs. ExPASy TrEMBL
Match: A0A6J1BSZ1 (uncharacterized protein LOC111005481 OS=Momordica charantia OX=3673 GN=LOC111005481 PE=4 SV=1)

HSP 1 Score: 271 bits (692), Expect = 4.85e-86
Identity = 129/248 (52.02%), Postives = 169/248 (68.15%), Query Frame = 0


Query: 5   NLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLLLA 64
           NLL +W+ FKLTSEED+IA+D+D  A++   + L  SL+ KLL+KR IS  VL   L +A
Sbjct: 5   NLLEEWKNFKLTSEEDKIAVDIDSSALEGTGKCLELSLICKLLSKRSISCTVLKNTLKIA 64

Query: 65  WKVEHQ-LTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEF 124
           WK++ +  +V+ IG N+FLF+F R  D NR+++ GPW FD+ALI++  P S     +++F
Sbjct: 65  WKLDCKAFSVDIIGFNIFLFNFNRSSDRNRILRMGPWTFDRALIIIDNPVSLTKPLDMDF 124

Query: 125 NRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPL 184
             V+ W+H FDL ++ +NKTMA RLGNAIG F DV+ N   F WG+ LR+RV  D+ KPL
Sbjct: 125 RNVSLWVHFFDLSLACMNKTMATRLGNAIGLFEDVESNANNFCWGSCLRVRVRFDVMKPL 184

Query: 185 RRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSEYGP 244
            RGIK+N+DGPMGGCWIPIQYERLPDF Y CG + H   DC        D      +YGP
Sbjct: 185 HRGIKLNLDGPMGGCWIPIQYERLPDFRYHCGRLDHILKDCSD---CCVDSVSKNLQYGP 244

Query: 245 WLRFVGSK 251
           WLRF G K
Sbjct: 245 WLRFQGHK 249

[Close]

BLAST of MC01g_new0248 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 252 bits (644), Expect = 4.67e-71
Identity = 118/247 (47.77%), Postives = 162/247 (65.59%), Query Frame = 0


Query: 5   NLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLLLA 64
           +LL +W+ FKLTSEE+E A+DVD  A       L   LVGKL  KR I+  V+   +  A
Sbjct: 5   DLLEEWKNFKLTSEEEETAIDVDASAPATTGSRLEQILVGKLFIKRPITCPVMKNTMRTA 64

Query: 65  WKVEHQ-LTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEF 124
           WK+E+    V+S+G NLFLF F R  D N++ K+GPW FD+ L+++ KP +    SEL+F
Sbjct: 65  WKLENNAFEVQSLGYNLFLFSFARALDRNKIYKSGPWTFDRTLVLINKPVALIPPSELDF 124

Query: 125 NRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPL 184
            ++  W+  FDLP+  + + MAIRLGNA+G F + DC++    WG++LR+RV++DI+KPL
Sbjct: 125 TKLPIWVRFFDLPLGCITRDMAIRLGNALGGFEEADCDDLNPDWGSNLRVRVMLDISKPL 184

Query: 185 RRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSEYGP 244
           RRGIK+N+DGP+GG WIPIQYERLPDFCY CG+                  SR   +YG 
Sbjct: 185 RRGIKLNLDGPIGGAWIPIQYERLPDFCYHCGL----------------SSSRKKHQYGS 235

Query: 245 WLRFVGS 250
           WLR+ G+
Sbjct: 245 WLRYQGT 235

[Close]

BLAST of MC01g_new0248 vs. ExPASy TrEMBL
Match: A0A1R3K847 (Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_10561 PE=4 SV=1)

HSP 1 Score: 187 bits (475), Expect = 3.48e-51
Identity = 94/246 (38.21%), Postives = 152/246 (61.79%), Query Frame = 0


Query: 4   ENLLADWQKFKLTSEED-EIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLL 63
           E L A W+ F LT EE  E+A++  + A  +AE  L   L+GKLL++R ++ DV+  VL 
Sbjct: 3   EGLSALWENFNLTKEETMEMAVNHTMVAESLAEGKLC--LIGKLLSRRAVNIDVMRNVLH 62

Query: 64  LAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELE 123
           + WK+   L V  IG+ L++F F  E +  RV + GPW F+KAL+VL+   +  ++ E++
Sbjct: 63  MVWKLSGGLQVREIGEKLYIFQFESEVEKERVYQQGPWNFNKALLVLKNYDAFISVEEIK 122

Query: 124 FNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVD-CNEKGFSWGASLRIRVLIDITK 183
               AFWI + DLP+ ++ +++   +G++ G  +++D C +K  +WG  LRIR  +++ K
Sbjct: 123 LEWCAFWIQVHDLPLGFMTESVGKAIGDSFGEVIEIDTCGDK-VAWGKFLRIRACLNVNK 182

Query: 184 PLRRGIKINIDGPMGG-CWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSE 243
           PLRRG+ +    P GG   +  +YE+LPDFCY CG + H+ ++C+   +  +D  +   E
Sbjct: 183 PLRRGMILT--APNGGKILVSFRYEKLPDFCYVCGCLNHTENECEKAVIMRRDSGKVKKE 242

Query: 244 YGPWLR 246
           YGPWLR
Sbjct: 243 YGPWLR 243

[Close]

BLAST of MC01g_new0248 vs. ExPASy TrEMBL
Match: A0A803MW53 (Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1)

HSP 1 Score: 188 bits (477), Expect = 8.85e-50
Identity = 91/245 (37.14%), Postives = 148/245 (60.41%), Query Frame = 0


Query: 4   ENLLADWQKFKLTSEEDEIAMDVDVDAVK-MAEQGLAYSLVGKLLAKRIISADVLSRVLL 63
           E+L+ DW++ KLT EE+ I    DV   + M+   ++ SLVGKLL K+  + + + RVL 
Sbjct: 3   ESLMKDWERLKLTDEENVIFGQSDVSLTEEMSNTKISLSLVGKLLTKKSFNVEAMKRVLK 62

Query: 64  LAWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELE 123
             W+++  + +  +  N+F+F F    D  +V++  PW FD  +++L++ C+++  SE+ 
Sbjct: 63  TLWRIDDNVAIRGVNTNIFVFQFFNIDDRKKVLEGRPWVFDNQILLLKEMCATEQPSEVS 122

Query: 124 FNRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKP 183
           F+   FWI L D+P    +  +A  +GN IG  +DVD ++    W   +R++V+++I KP
Sbjct: 123 FDACPFWIRLLDVPFGMRSSRVAHEIGNCIGECIDVDESDP-LCWDEFMRVKVMVNINKP 182

Query: 184 LRRGIKINIDGPMGGC--WIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDDSRATSE 243
           LRRG+KI ++    GC  WI  +YERL DFCY+CG IGH+  DC+ +     D S    +
Sbjct: 183 LRRGMKIAVED---GCAKWIGFKYERLGDFCYYCGRIGHTDKDCEQKD-GGSDVSLIVFQ 242

Query: 244 YGPWL 245
           YGP+L
Sbjct: 243 YGPFL 242

[Close]

BLAST of MC01g_new0248 vs. TAIR 10
Match: AT3G31430.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G18636.1); Has 295 Blast hits to 291 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 295; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 73.2 bits (178), Expect = 5.2e-13
Identity = 42/144 (29.17%), Postives = 68/144 (47.22%), Query Frame = 0


Query: 81  FLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEFNRVAFWIHLFDLPMSWL 140
           F F F  E  +  V++ GPW F+  +I+LQ+      I    F  + FW+ +  +P  +L
Sbjct: 173 FHFIFTLEESLETVLRRGPWAFNDWMILLQR--WEPQIPLFPF--IPFWVQIRGIPFQFL 232

Query: 141 NKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPLRRGIKINIDGPMGGCWI 200
           N+ +   +G A+G  +D D N +  +     R+ +  DIT PLR          +    +
Sbjct: 233 NRGVVEHIGRALGQVLDTDFNVEVVARMDFARVLLHWDITHPLRFQRHFQFTAGV-NTLL 292

Query: 201 PIQYERLPDFCYFCGVIGHSSHDC 225
             +YERL  FC  CG++ H    C
Sbjct: 293 RFRYERLRGFCEVCGMLTHDFGAC 311

[Close]

BLAST of MC01g_new0248 vs. TAIR 10
Match: AT5G36228.1 (nucleic acid binding;zinc ion binding )

HSP 1 Score: 71.2 bits (173), Expect = 2.0e-12
Identity = 53/224 (23.66%), Postives = 97/224 (43.30%), Query Frame = 0


Query: 11  QKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLLLAWKVEHQ 70
           Q   L  EE E+ +        +A   L  SL+G++L  +  S +     L   W +  Q
Sbjct: 10  QHMDLGREEPELYIPYHAYVGALASNRL--SLLGRILNPQTQSVERAILELPYQWGLGTQ 69

Query: 71  LTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEFNRVAFWI 130
           +    +    F   F  E D+   ++  PW F++  I LQ+         L F  +  W+
Sbjct: 70  VHGRILDDRCFQVRFRSEIDLLNGLRRAPWVFNEWFIALQRWEDFPTEDFLTF--IDVWV 129

Query: 131 HLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPLRRGIKIN 190
           H+  +P+ ++++     + + +G  V +D NE+  S    +R++V +D T+PLR   ++ 
Sbjct: 130 HIRGIPLPYVSERTVEIIASTLGEVVAMDFNEETTSQITFIRVKVRMDFTEPLRFFRRVR 189

Query: 191 IDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDCDARYLAAQDD 235
                    I  +YE+L   C  C  + H    C   Y+  Q++
Sbjct: 190 F-ASRERAMIGFEYEKLQRVCTNCCRVNHQVSHCP--YVVHQEE 226

[Close]

BLAST of MC01g_new0248 vs. TAIR 10
Match: AT3G42140.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 62.8 bits (151), Expect = 7.1e-10
Identity = 51/221 (23.08%), Postives = 96/221 (43.44%), Query Frame = 0


Query: 4   ENLLADWQKFKLTSEEDEIAMDVDVDAVKMAEQGLAYSLVGKLLAKRIISADVLSRVLLL 63
           +NL    Q+  L  +E+ I + +++    + +  LA SL  +    + +  +V+ R+L  
Sbjct: 3   DNLQRQMQEIALGVQEEAINLLIELCDEALRKPNLA-SLQNR--CPKNVDEEVVGRIL-- 62

Query: 64  AWKVEHQLTVESIGKNLFLFHFCRECDMNRVMKTGPWFFDKALIVLQKPCSSKNISELEF 123
                       I K  FLF    E  M  +++ GPW F+  + V+Q+   +K  S+ EF
Sbjct: 63  -----------EIHKIEFLFQ--SEESMFSILRRGPWSFNDWMCVIQR--WTKLHSDAEF 122

Query: 124 NRVAFWIHLFDLPMSWLNKTMAIRLGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPL 183
            R+ FWI +  +P+ +L   +   +G  +G F++ +                       L
Sbjct: 123 KRIPFWIQIRGIPLRFLTARIITSIGERMGLFLETN-----------------------L 170

Query: 184 RRGIKINIDGPMGGCWIPIQYERLPDFCYFCGVIGHSSHDC 225
            R + +          +  QYE+L +FC  CG++ H + +C
Sbjct: 183 GRDVSV----------LKFQYEKLKNFCTTCGMLSHDASEC 170

The following BLAST results are available for this feature:

BLAST of MC01g_new0248 vs.


Match Name	E-value	Identity	Description

BLAST of MC01g_new0248 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156185.1	8.70e-284	100.00	uncharacterized protein LOC111023135 [Momordica charantia] >XP_022156186.1 uncha...	[more]
XP_022132681.1	1.00e-85	52.02	uncharacterized protein LOC111005481 [Momordica charantia]	[more]
XP_022158377.1	9.65e-71	47.77	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_028117212.1	2.05e-54	40.00	uncharacterized protein LOC114314884 [Camellia sinensis]	[more]
XP_028122006.1	2.37e-51	38.65	uncharacterized protein LOC114319195 [Camellia sinensis]	[more]

BLAST of MC01g_new0248 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DU55	4.21e-284	100.00	uncharacterized protein LOC111023135 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1BSZ1	4.85e-86	52.02	uncharacterized protein LOC111005481 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1DX30	4.67e-71	47.77	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A1R3K847	3.48e-51	38.21	Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_10561 PE=4 SV=1	[more]
A0A803MW53	8.85e-50	37.14	Uncharacterized protein OS=Chenopodium quinoa OX=63459 PE=4 SV=1	[more]

BLAST of MC01g_new0248 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G31430.1	5.2e-13	29.17	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT5G36228.1	2.0e-12	23.66	nucleic acid binding;zinc ion binding	[more]
AT3G42140.1	7.1e-10	23.08	zinc ion binding;nucleic acid binding	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 274..294
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 252..317
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 286..305
None	No IPR available	PANTHER	PTHR31286:SF84	SUBFAMILY NOT NAMED	coord: 15..285
IPR025558	Domain of unknown function DUF4283	PFAM	PF14111	DUF4283	coord: 32..175 e-value: 4.6E-25 score: 87.8
IPR025836	Zinc knuckle CX2CX4HX4C	PFAM	PF14392	zf-CCHC_4	coord: 177..225 e-value: 4.4E-15 score: 55.1
IPR040256	Uncharacterized protein At4g02000-like	PANTHER	PTHR31286	GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKE	coord: 15..285
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 211..224 score: 8.762648

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g_new0248.1	MC01g_new0248.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding