MC01g_new0244 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g_new0244
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionspermidine hydroxycinnamoyl transferase-like
LocationMC01: 9756294 .. 9759139 (-)
RNA-Seq ExpressionMC01g_new0244
SyntenyMC01g_new0244
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAGCTAAGAAACCTTAATCTCACTTAAATATTTTCAAGAGGAAGACTATAATATTTAAAGAAAGAAAAACTATAAATGAAATGATTTATGAAATCAACTCCATTCATAAAATTTGATAAAAAAAAAAGAATAATCCTCCCAATCCAAGCTCTCGTGAAAACCAGAATCAATGGCGTCTTTAACCATTAATTCTTCCACCACCGTCTTTCCCAGCCACCCTACTCCCGCCGCCGCTCTTTCCTTATCGGAGAACGACCAGTTCAGGGCATGGACTCACGTCCCCACCGTCTACATCTACAAATCTCCGGCTGCCGCGGCCACCGCTGCCGTCCTCGGTTCCTTAAAAAACTCCCTCAGCCAAATCCTCGTCCCTTACTACCCTCTCGCCGGCCGCCTCCGCTTGATCGCTGGCGGCCGCCTCGAGCTTGACTGCTGCGCCGCCGGAGCCACACTCATGGAAGCTTCCTCCACTGCCAAGCTCGACGACTACGGCGACTTCGCACCCTCCGATGCTCTCCGACGACTCGCTCCCAACGTCGATTACGATAATTCTCCCATCGAGGAGGTACCGATGCTGCTGGTTCAGGTGACGAGATTCAGCTGCGGCGGGCTTGTCGTCGGCGTCACCTTTTCTCACACTCTCGTCGACGGGGCCTCCGCCATTACGTTCATCAACTCGTGGGCGAGCATGGCGCGCGGCGAGAAAACGGTGGACAGTATTCCGCAGCCGTTTCATGACAGGAAGTTTTTGCAACCGCAGAAGCCGGTTCCTCCACCGCGGTTTCACCATGGTGAATATGATGAACCACCGCTTCTCCATGGATACTCCAATGCCGAGGTAATTAAGCTTTAGCAAATAGGTTACAATATACATGATCATTTTTGTAAAGTTACAAATTTCTTTTTTATTGTTTAAATATAATTTTAATTGTACGGAATGAATTATTTTTTAATCTATTTTTAATAGGAGATCGAACCCACACTCTCTTAGTCGGAGATATATGACAATTACCGTTGAGCTATACTCGCTTTTGGTTTATTTTAATCTTATCAATCTTGATTTTCTAAATAACAAAATTATTCTTTACTTTTCGCTCTAGGTTATCTCTTTCACCTCATACAATTACTTATCCGGAATTGAAATAGGAAATCTTAACTGGTATGTTGATTGTTAGAAGTTGAAAATCTATTGATCATGTTAATTTCTAAAACTATCTGTAACCTAAATGAGTCAACCTATAACAATTATTAGAACATCTCTTCCTAATTTTTAAATAATTAGAAATACATTAATATTAATATTATTTTTCAAATCAATAATAAATTTTAATTTATTTTATTAGTATTGTATTGATAGCAATATGTTACTTTAATATTATAAATAATATATAATTAAAATAAAATTATTGAGTCAAACTTGTATTAGAATTTAGGATCTTGTAAAATATAAAAGAGAAAAATGCCTCTTCTTAGTCTAAAGTTTTTGCTAATAGGACACACTTCTTTTAATTTGTTCTAGTTCTGAAAAGCCACGTTTCCCCTCTGGGTTTTAAAAAATCAGTTGTGTCCAACGAATACCTTTTGAATTAGTTAATGGTGACCGGATAATTTCTTTTATTTACCTATAATATTAATGTGAAATTATATATTTTAGTATTCTTATTGCTGGTATGTTATTTAAATATTTCTTGAGAAATAATAAAATATATATTTTTTGTCCGTGAGCCAAACTGAACCTAGCTCAAATGGAAAAATATATTAATTTAATAATATTTAAACTAATAAAATATTTTATTGATATTCATAATAGGAGGAGGAGAAGAAAGAATCTGTTTGTGCATTGCTAAAACTTACAAAGGAACAAGTTGAAAAGTTGAAAAAGAGAGCAAATTCGAACCAACCAAATCCAAATACAACTAATCAAAACATGGCAATGCTTGATCATGAACTGCAGCCACGACCATACAGTCGATATGAGTCTATCACTGGATACATGTGGTTGTGTGCATGTAAGGCCCGTAACAATGCTGAGAGTAGACAGCCGACAGTGGTCCGTATCATCGTCGATATAAGTCGACGACTAAGGACACCCATCCCCGAAAACTTCACTGGAAACTCATGTCTGATCACGATAACACCGCAATGCAAGTTTGGTGATCTGATGACTCAACCACTAAGCTATGCTGTTGGAAAAATAAGAGAAGGAACGTGGAAGATGACCGACGAATACGCAAAGTCGGCAATGGACTTTCTGGCTAGGAAAGAGGACATCACCTGGGCAAGAACATTGCACCACTCCATAGTTCGGGTCAAAGGTTCATTTTGGGGCAACCCGAATCTAGCTTTAGGGAGTTGGATGTCGATGCCGTTGTATGATGCAGATTTTGGGTGGGGACGACCATCTTACGTTGGGCCGGCAATGGTGAACAGTGATGGAAAGGCGTACATCATGTCGGGGCCAACCAATGATGGGTCGGTCATCATAGCGATACGATTGCAGACAAAGCATATGGATGATTTCAAGAAGTTCTTTTATGAAGATCTGTGAGAAACCAAGGATCGATCATTAATGGAATAAAATAGAATAATCTTTGCATCTAACGATGCAAGTGTTGTCGCCATTAATTTTTTCTTTTTACAAGTGTCTGAACTAGCTTTACGTGTACCTCAGTTTTCACCATTATTTATTACATATGGTTAGATATCTTATAAATGATTAAATTTACCCTAATTCATTAGCTTAAGCGTTTTTGGGTTCATTAGTGATTTAACCATATTTGTGTTAGAAACCCTATATTTTTATTTCCTCTCTATTTAACTTTTGGGTTTATTGGTGATTTAATC

mRNA sequence

ATGGCGTCTTTAACCATTAATTCTTCCACCACCGTCTTTCCCAGCCACCCTACTCCCGCCGCCGCTCTTTCCTTATCGGAGAACGACCAGTTCAGGGCATGGACTCACGTCCCCACCGTCTACATCTACAAATCTCCGGCTGCCGCGGCCACCGCTGCCGTCCTCGGTTCCTTAAAAAACTCCCTCAGCCAAATCCTCGTCCCTTACTACCCTCTCGCCGGCCGCCTCCGCTTGATCGCTGGCGGCCGCCTCGAGCTTGACTGCTGCGCCGCCGGAGCCACACTCATGGAAGCTTCCTCCACTGCCAAGCTCGACGACTACGGCGACTTCGCACCCTCCGATGCTCTCCGACGACTCGCTCCCAACGTCGATTACGATAATTCTCCCATCGAGGAGGTACCGATGCTGCTGGTTCAGGTGACGAGATTCAGCTGCGGCGGGCTTGTCGTCGGCGTCACCTTTTCTCACACTCTCGTCGACGGGGCCTCCGCCATTACGTTCATCAACTCGTGGGCGAGCATGGCGCGCGGCGAGAAAACGGTGGACAGTATTCCGCAGCCGTTTCATGACAGGAAGTTTTTGCAACCGCAGAAGCCGGTTCCTCCACCGCGGTTTCACCATGGTGAATATGATGAACCACCGCTTCTCCATGGATACTCCAATGCCGAGGAGGAGGAGAAGAAAGAATCTGTTTGTGCATTGCTAAAACTTACAAAGGAACAAGTTGAAAAGTTGAAAAAGAGAGCAAATTCGAACCAACCAAATCCAAATACAACTAATCAAAACATGGCAATGCTTGATCATGAACTGCAGCCACGACCATACAGTCGATATGAGTCTATCACTGGATACATGTGGTTGTGTGCATGTAAGGCCCGTAACAATGCTGAGAGTAGACAGCCGACAGTGGTCCGTATCATCGTCGATATAAGTCGACGACTAAGGACACCCATCCCCGAAAACTTCACTGGAAACTCATGTCTGATCACGATAACACCGCAATGCAAGTTTGGTGATCTGATGACTCAACCACTAAGCTATGCTGTTGGAAAAATAAGAGAAGGAACGTGGAAGATGACCGACGAATACGCAAAGTCGGCAATGGACTTTCTGGCTAGGAAAGAGGACATCACCTGGGCAAGAACATTGCACCACTCCATAGTTCGGGTCAAAGGTTCATTTTGGGGCAACCCGAATCTAGCTTTAGGGAGTTGGATGTCGATGCCGTTGTATGATGCAGATTTTGGGTGGGGACGACCATCTTACGTTGGGCCGGCAATGGTGAACAGTGATGGAAAGGCGTACATCATGTCGGGGCCAACCAATGATGGGTCGGTCATCATAGCGATACGATTGCAGACAAAGCATATGGATGATTTCAAGAAGTTCTTTTATGAAGATCTGTGA

Coding sequence (CDS)

ATGGCGTCTTTAACCATTAATTCTTCCACCACCGTCTTTCCCAGCCACCCTACTCCCGCCGCCGCTCTTTCCTTATCGGAGAACGACCAGTTCAGGGCATGGACTCACGTCCCCACCGTCTACATCTACAAATCTCCGGCTGCCGCGGCCACCGCTGCCGTCCTCGGTTCCTTAAAAAACTCCCTCAGCCAAATCCTCGTCCCTTACTACCCTCTCGCCGGCCGCCTCCGCTTGATCGCTGGCGGCCGCCTCGAGCTTGACTGCTGCGCCGCCGGAGCCACACTCATGGAAGCTTCCTCCACTGCCAAGCTCGACGACTACGGCGACTTCGCACCCTCCGATGCTCTCCGACGACTCGCTCCCAACGTCGATTACGATAATTCTCCCATCGAGGAGGTACCGATGCTGCTGGTTCAGGTGACGAGATTCAGCTGCGGCGGGCTTGTCGTCGGCGTCACCTTTTCTCACACTCTCGTCGACGGGGCCTCCGCCATTACGTTCATCAACTCGTGGGCGAGCATGGCGCGCGGCGAGAAAACGGTGGACAGTATTCCGCAGCCGTTTCATGACAGGAAGTTTTTGCAACCGCAGAAGCCGGTTCCTCCACCGCGGTTTCACCATGGTGAATATGATGAACCACCGCTTCTCCATGGATACTCCAATGCCGAGGAGGAGGAGAAGAAAGAATCTGTTTGTGCATTGCTAAAACTTACAAAGGAACAAGTTGAAAAGTTGAAAAAGAGAGCAAATTCGAACCAACCAAATCCAAATACAACTAATCAAAACATGGCAATGCTTGATCATGAACTGCAGCCACGACCATACAGTCGATATGAGTCTATCACTGGATACATGTGGTTGTGTGCATGTAAGGCCCGTAACAATGCTGAGAGTAGACAGCCGACAGTGGTCCGTATCATCGTCGATATAAGTCGACGACTAAGGACACCCATCCCCGAAAACTTCACTGGAAACTCATGTCTGATCACGATAACACCGCAATGCAAGTTTGGTGATCTGATGACTCAACCACTAAGCTATGCTGTTGGAAAAATAAGAGAAGGAACGTGGAAGATGACCGACGAATACGCAAAGTCGGCAATGGACTTTCTGGCTAGGAAAGAGGACATCACCTGGGCAAGAACATTGCACCACTCCATAGTTCGGGTCAAAGGTTCATTTTGGGGCAACCCGAATCTAGCTTTAGGGAGTTGGATGTCGATGCCGTTGTATGATGCAGATTTTGGGTGGGGACGACCATCTTACGTTGGGCCGGCAATGGTGAACAGTGATGGAAAGGCGTACATCATGTCGGGGCCAACCAATGATGGGTCGGTCATCATAGCGATACGATTGCAGACAAAGCATATGGATGATTTCAAGAAGTTCTTTTATGAAGATCTGTGA

Protein sequence

MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKNSLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLAPNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKTVDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKEQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMTDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRPSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL
Homology
BLAST of MC01g_new0244 vs. ExPASy Swiss-Prot
Match: O64470 (Spermidine hydroxycinnamoyl transferase OS=Arabidopsis thaliana OX=3702 GN=SHT PE=1 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 2.6e-96
Identity = 185/472 (39.19%), Postives = 283/472 (59.96%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MA +T   S T+ P+ PT +    L+E DQ    TH+PT+Y Y  P+ +    V+  LK 
Sbjct: 1   MAPITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNVVEILKT 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLS++LV +YP+AGRLR +  GR EL+C A G   +EA S  KL D+ DF+P+     L 
Sbjct: 61  SLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFSPTPEFENLM 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P V+Y N PIE +P+ L QVT+F CGG+ + V  SH +VDG SA+  I+ W  +ARGE  
Sbjct: 121 PQVNYKN-PIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGE-P 180

Query: 181 VDSIPQPFHDRKFLQPQKPVP----PPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLK 240
           ++++  PF DRK L   +P+P    PP+F H E+D+PP L G ++  EE KK+++  +L 
Sbjct: 181 LETV--PFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLP 240

Query: 241 LTKEQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNA 300
           L+  Q++KL+ +AN ++              H    + ++RYE++TG++W CACKAR ++
Sbjct: 241 LSTSQLQKLRSKANGSK--------------HSDPAKGFTRYETVTGHVWRCACKARGHS 300

Query: 301 ESRQPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGT 360
              QPT + I +D   R+  P+P  + GN+ L  +      G+L++  L +A   I +  
Sbjct: 301 -PEQPTALGICIDTRSRMEPPLPRGYFGNATLDVVAASTS-GELISNELGFAASLISKAI 360

Query: 361 WKMTDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFG 420
             +T+EY    +++L  ++D+   + L H++   +G F+GNPNL + SW+++P+Y  DFG
Sbjct: 361 KNVTNEYVMIGIEYLKNQKDLKKFQDL-HALGSTEGPFYGNPNLGVVSWLTLPMYGLDFG 420

Query: 421 WGRPSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 469
           WG+  Y GP   + DG + I+     DGSVI+A  LQ  HM+ FKK FYED+
Sbjct: 421 WGKEFYTGPGTHDFDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYEDI 451

BLAST of MC01g_new0244 vs. ExPASy Swiss-Prot
Match: Q8GSM7 (Shikimate O-hydroxycinnamoyltransferase OS=Nicotiana tabacum OX=4097 GN=HST PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 5.2e-76
Identity = 175/465 (37.63%), Postives = 256/465 (55.05%), Query Frame = 0

Query: 4   LTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKNSLS 63
           + +  ST V P+  TP   L  S  D      H P+VY Y+ P  +        LK +LS
Sbjct: 3   IEVKESTMVKPAAETPQQRLWNSNVDLVVPNFHTPSVYFYR-PTGSPNFFDGKVLKEALS 62

Query: 64  QILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLAPNV 123
           + LVP+YP+AGRL     GR+E+DC   G   +EA S   +DD+GDFAP+  LR+L P V
Sbjct: 63  KALVPFYPMAGRLCRDEDGRIEIDCKGQGVLFVEAESDGVVDDFGDFAPTLELRQLIPAV 122

Query: 124 DYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKTVDS 183
           DY    I+   +L++Q+T F CGG+ +GV   H   DGAS + FIN+W+ MARG   +D 
Sbjct: 123 DYSQG-IQSYALLVLQITHFKCGGVSLGVGMQHHAADGASGLHFINTWSDMARG---LDL 182

Query: 184 IPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLH--GYSNAEEEEKKESVCALLKLTKEQ 243
              PF DR  L+ + P P P+F H EY  PP L     +    E   E+  ++ KLT++Q
Sbjct: 183 TIPPFIDRTLLRARDP-PQPQFPHVEYQPPPTLKVTPENTPISEAVPETSVSIFKLTRDQ 242

Query: 244 VEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQP 303
           +  LK +   ++ + NT N              YS YE + G++W   C AR  A   Q 
Sbjct: 243 INTLKAK---SKEDGNTVN--------------YSSYEMLAGHVWRSTCMARGLAHD-QE 302

Query: 304 TVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMTD 363
           T + I  D   RLR  +P  + GN  + T TP    GD+ ++P+ YA  K+ +   +M +
Sbjct: 303 TKLYIATDGRSRLRPSLPPGYFGN-VIFTTTPIAVAGDIQSKPIWYAASKLHDALARMDN 362

Query: 364 EYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRPS 423
           +Y +SA+D+L  + D+        ++VR   +F   PNL + SW  +P++DADFGWGRP 
Sbjct: 363 DYLRSALDYLELQPDL-------KALVRGAHTF-KCPNLGITSWSRLPIHDADFGWGRPI 422

Query: 424 YVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYE 467
           ++GP  +  +G ++I+  PTNDGS  +AI LQ +HM  F+KF Y+
Sbjct: 423 FMGPGGIAYEGLSFILPSPTNDGSQSVAISLQAEHMKLFEKFLYD 434

BLAST of MC01g_new0244 vs. ExPASy Swiss-Prot
Match: Q9FI78 (Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.1e-75
Identity = 172/464 (37.07%), Postives = 261/464 (56.25%), Query Frame = 0

Query: 4   LTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKNSLS 63
           + I  ST V P+  TP   L  S  D      H P+VY Y+ P  A+       +K +LS
Sbjct: 3   INIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYR-PTGASNFFDPQVMKEALS 62

Query: 64  QILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLAPNV 123
           + LVP+YP+AGRL+    GR+E+DC  AG   + A + + +DD+GDFAP+  LR+L P V
Sbjct: 63  KALVPFYPMAGRLKRDDDGRIEIDCNGAGVLFVVADTPSVIDDFGDFAPTLNLRQLIPEV 122

Query: 124 DYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKTVDS 183
           D+ ++ I   P+L++QVT F CGG  +GV   H   DG S + FIN+W+ MARG   +D 
Sbjct: 123 DH-SAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMARG---LDL 182

Query: 184 IPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKEQVE 243
              PF DR  L+ + P P P FHH EY   P +    +  +   + +  ++ KLT++Q+ 
Sbjct: 183 TIPPFIDRTLLRARDP-PQPAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLV 242

Query: 244 KLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQPTV 303
            LK +   ++ + NT +              YS YE + G++W    KAR    + Q T 
Sbjct: 243 ALKAK---SKEDGNTVS--------------YSSYEMLAGHVWRSVGKAR-GLPNDQETK 302

Query: 304 VRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMTDEY 363
           + I  D   RLR  +P  + GN  + T TP    GDL+++P  YA G+I +   +M D Y
Sbjct: 303 LYIATDGRSRLRPQLPPGYFGN-VIFTATPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNY 362

Query: 364 AKSAMDFLARKEDITWARTLHHSIVRVKGSF-WGNPNLALGSWMSMPLYDADFGWGRPSY 423
            +SA+D+L  + D++           V+G+  +  PNL + SW+ +P+YDADFGWGRP +
Sbjct: 363 LRSALDYLEMQPDLS---------ALVRGAHTYKCPNLGITSWVRLPIYDADFGWGRPIF 422

Query: 424 VGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYE 467
           +GP  +  +G ++++  PTNDGS+ +AI LQ++HM  F+KF +E
Sbjct: 423 MGPGGIPYEGLSFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of MC01g_new0244 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ1 (Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 6.5e-71
Identity = 164/470 (34.89%), Postives = 248/470 (52.77%), Query Frame = 0

Query: 4   LTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKNSLS 63
           + I  +T V PS PTP+  L  S  D      H P+VY Y+ P  A+       LK +L 
Sbjct: 3   INIKETTMVRPSQPTPSQRLWNSNLDLVVPRFHTPSVYFYRRPGNASDFFDARVLKEALG 62

Query: 64  QILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLAPNV 123
           + LVP+YP+AGRL     GR+E+DC   G   + A + + +D++GDFAP+  L++L P V
Sbjct: 63  RALVPFYPMAGRLARDEDGRVEIDCNGEGVRFVVAETDSAIDEFGDFAPTMELKKLIPKV 122

Query: 124 DYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKTVDS 183
           +Y +  I   P+L++Q+T F CGG  +GV   H + DGAS + FINSW+ +ARG   +D 
Sbjct: 123 EYGDD-ISAFPLLVLQITHFKCGGTSLGVGMQHHVADGASGLHFINSWSDIARG---LDI 182

Query: 184 IPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHG-----YSNAEEEEKKESVCALLKLT 243
              PF DR  L+ + P P P F H EY   P ++           + +   +  ++ KLT
Sbjct: 183 AVPPFIDRSLLRARDP-PSPSFPHIEYQPAPSMNTSPAPIQDPTVKSDPTATAVSIFKLT 242

Query: 244 KEQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAES 303
           K+Q++ LK R ++                       YS Y  + G++W C   AR   + 
Sbjct: 243 KQQLDLLKSRVSAK----------------------YSSYALVAGHVWRCTSIARGLPDD 302

Query: 304 RQPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQP--LSYAVGKIREGT 363
            Q T +    D   RL+ P+P  + GN  + T TP    G++  +   L  A G+I+   
Sbjct: 303 -QRTKLYCATDGRARLQPPLPSGYFGN-VIFTATPVADAGEITGEDSGLEAAAGRIQRAL 362

Query: 364 WKMTDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFG 423
            +M DEY +SA+D+L  + D++        +VR   +F   PN+ L SW  +P++DADFG
Sbjct: 363 MRMDDEYLRSALDYLELQPDLS-------KLVRGAHTF-RCPNIGLTSWTRLPIHDADFG 422

Query: 424 WGRPSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYE 467
           WGRP ++GP  +  +G A+++     DGS+ IAI LQ +HM  F+K  YE
Sbjct: 423 WGRPIFMGPGGIAYEGLAFMLPSSEGDGSLSIAISLQAEHMIKFQKLLYE 435

BLAST of MC01g_new0244 vs. ExPASy Swiss-Prot
Match: Q0JBZ8 (Hydroxycinnamoyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=HCT1 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.1e-65
Identity = 162/473 (34.25%), Postives = 246/473 (52.01%), Query Frame = 0

Query: 3   SLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYK---SPAAAATAAVLGS-L 62
           ++T+  ST V P+  TP   L  S  D      H P+VY Y+       A      G  +
Sbjct: 2   AITVRRSTMVRPAWETPRVRLWNSNLDLVVPRFHTPSVYFYRRGPEGGGAPEGFFDGERM 61

Query: 63  KNSLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASS-TAKLDDYGDFAPSDALR 122
           + +L++ LVP+YP+AGRL     GR+E+DC   G   +EA +  A +DDYGDFAP+  L+
Sbjct: 62  RRALAEALVPFYPMAGRLARDEDGRVEIDCNGEGVLFVEADAPDASVDDYGDFAPTMELK 121

Query: 123 RLAPNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARG 182
           RL P VDY +  I    +L++QVT F CGG+ +GV   H + DG S + FINSW+ + RG
Sbjct: 122 RLIPAVDYTDD-ISSFSLLVLQVTYFKCGGVSLGVGMQHHVADGMSGLHFINSWSDLCRG 181

Query: 183 EKTVDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHG----YSNAEEEEKKESVCA 242
            +       PF DR  L+ + P P P + H EY   P +         A +     +   
Sbjct: 182 TQIA---IMPFIDRTLLRARDP-PTPSYPHVEYQPAPAMLSSVPQSVTANKTTPPPTAVD 241

Query: 243 LLKLTKEQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKAR 302
           + KLT+  + +L+ +  S +  P                  +S Y  +  ++W C   AR
Sbjct: 242 IFKLTRSDLGRLRSQLPSGEGAPR-----------------FSTYAVLAAHVWRCVSLAR 301

Query: 303 NNAESRQPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIR 362
               S QPT +    D  +RL+ P+PE + GN  + T TP  + G  +T  L+     I+
Sbjct: 302 -GLPSEQPTKLYCATDGRQRLQPPLPEGYFGN-VIFTATPLAEAGK-VTSGLADGAAVIQ 361

Query: 363 EGTWKMTDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDA 422
           E   +M D Y +SA+D+L  + D++       ++VR   +F   PNL L SW+ +P++DA
Sbjct: 362 EALDRMNDSYCRSALDYLELQPDLS-------ALVRGAHTF-RCPNLGLTSWVRLPIHDA 421

Query: 423 DFGWGRPSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYE 467
           DFGWGRP ++GP  +  +G A+++     DGS+ IAI LQ +HM+ F+K  +E
Sbjct: 422 DFGWGRPVFMGPGGIAYEGLAFVLPSANKDGSLSIAISLQAEHMEKFRKLIFE 441

BLAST of MC01g_new0244 vs. NCBI nr
Match: XP_022156247.1 (spermidine hydroxycinnamoyl transferase-like [Momordica charantia])

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN
Sbjct: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA
Sbjct: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT
Sbjct: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240
           VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE
Sbjct: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240

Query: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300
           QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ
Sbjct: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300

Query: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360
           PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT
Sbjct: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360

Query: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420
           DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP
Sbjct: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420

Query: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
           SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL
Sbjct: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468

BLAST of MC01g_new0244 vs. NCBI nr
Match: XP_023544822.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 664 bits (1712), Expect = 3.84e-236
Identity = 323/467 (69.16%), Postives = 388/467 (83.08%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MASLTI+SSTTVFP+HPTP A LS SE+DQ + WTH PTVY+Y+SPA A T+ V+ SL+N
Sbjct: 1   MASLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPAVA-TSTVVESLRN 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQ+LVPYYPLAGRL  I GGRLEL CC+AGA L+EASSTA LDDYGDF+P+DALR LA
Sbjct: 61  SLSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELA 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P VDY ++PIEE+P+LLVQ+TRFSCGG+V+G+  SH LVDG SAITFINSWAS+AR EKT
Sbjct: 121 PKVDY-STPIEELPVLLVQITRFSCGGIVIGIGISHILVDGVSAITFINSWASIARKEKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240
           V+SI QPFHDR  LQPQKP+ PPRF HGEYD PP L G  +A+EE KKES   LLKL+KE
Sbjct: 181 VESIVQPFHDRSILQPQKPLRPPRFDHGEYDNPPFLLGCLDAKEERKKESTVTLLKLSKE 240

Query: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300
           QVEKLKKRANSN          +  LD E+  RPY+RYES+ G+MW+CACKARN  ++ Q
Sbjct: 241 QVEKLKKRANSN---------TVTQLD-EVPSRPYTRYESVAGHMWVCACKARNTNDNSQ 300

Query: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360
           PT+VR+ VD+ RRLRTPIP NFTGNS L+ +TP+C+F DL++QPLSYA GKIREGTWK+T
Sbjct: 301 PTMVRVAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFNDLISQPLSYAAGKIREGTWKIT 360

Query: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420
           DEYA+SA+DFLA +EDI+W RT +H+ V+V+  FWGNPNL+LGSWMS+PLY+ADFGWG P
Sbjct: 361 DEYARSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGPP 420

Query: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYED 467
            YVGPA +N+DGK++IM  P NDG ++IA+RLQ KHMDDFK+ FYED
Sbjct: 421 CYVGPATLNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 455

BLAST of MC01g_new0244 vs. NCBI nr
Match: KAG6603487.1 (Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 662 bits (1707), Expect = 2.29e-235
Identity = 325/468 (69.44%), Postives = 391/468 (83.55%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MASLTI+SSTTVFP+HPTP A LS SE+DQ + WTH PTVY+Y+SPA   T+AV+ SL+N
Sbjct: 1   MASLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPAVT-TSAVVESLRN 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQ+LVPYYPLAGRL  I GGRLEL CC+AGA L+EASSTA LDDYGDF+P+DALR LA
Sbjct: 61  SLSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELA 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P VDY ++PIEE+P+LLVQ+TRFSCGG+V+G+  SH LVDG SAITFINSWAS+AR EKT
Sbjct: 121 PKVDY-STPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARKEKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240
           V+SI  PFHDR  LQPQKP+ PPRF H EYD PP L G S+A+EE KKES+  LLKL+KE
Sbjct: 181 VESIVLPFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLKLSKE 240

Query: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300
           QVEKLKKRANSN          +  LD EL  RPY+RYESI G+MW+CACKAR+ +++ Q
Sbjct: 241 QVEKLKKRANSNI---------VTQLD-ELPSRPYTRYESIAGHMWVCACKARSTSDNSQ 300

Query: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360
           PT+VR+ VD+ RRL TP+P NFTGNS L+ +TP+C+FGDL++QPLSYA GKIREGTWK+T
Sbjct: 301 PTMVRVAVDVRRRLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKIT 360

Query: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420
           DEYA+SA+DFLA +EDI+W RT +H+ V+V+  FWGNPNL+LGSWMS+PLY+ADFGWG+P
Sbjct: 361 DEYARSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGKP 420

Query: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
            YVGPA +N+DGK++IM  P NDG +IIAIRLQ KHMDDFK+ FYEDL
Sbjct: 421 CYVGPATLNADGKSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYEDL 456

BLAST of MC01g_new0244 vs. NCBI nr
Match: KAG6595485.1 (Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661 bits (1706), Expect = 3.77e-235
Identity = 325/469 (69.30%), Postives = 393/469 (83.80%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MAS+TI+SS+TV PSHPTP  A S+SE DQ+RAWTH+P+VY+YKSPAA     ++ SLK 
Sbjct: 1   MASITIHSSSTVIPSHPTPTGAFSVSECDQYRAWTHLPSVYVYKSPAAEVI--IVESLKT 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQILVP+YPLAGRL  + GGRLELDCCAAGA  +EAS+ AKL+DYGDFAPSDA+R L 
Sbjct: 61  SLSQILVPFYPLAGRLHWLPGGRLELDCCAAGALFVEASTNAKLEDYGDFAPSDAVRELV 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P+VDY NSPIEE+P+LL QVTRFSCGG+V+GV   HT+VDGASA+TFINSWASMARGEKT
Sbjct: 121 PSVDY-NSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAVTFINSWASMARGEKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPP-LLHGYSNAEEEEKKESVCALLKLTK 240
           V++I QP HDRKFL P+KP+ PPRF H E+ +PP L+ G SN EEE KKE+  ALLKLTK
Sbjct: 181 VETILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPLILGSSNTEEEAKKETTSALLKLTK 240

Query: 241 EQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESR 300
           EQ+EKLKKRANSN      TNQ M + D E+QPRPYSR+E+ITG+MW+CACKARN  ESR
Sbjct: 241 EQIEKLKKRANSN-----LTNQTMDVAD-EVQPRPYSRFEAITGHMWVCACKARNTGESR 300

Query: 301 QPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKM 360
           QPTV+RI +D+ RR++ PIPENF+GNS  +  TP+C+FG+LM+QPLSYA GKIREGTWKM
Sbjct: 301 QPTVIRIPIDVRRRMKPPIPENFSGNSSFVVSTPECEFGELMSQPLSYAAGKIREGTWKM 360

Query: 361 TDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGR 420
           T EYA+SA+D+LA ++D    RT  HS V V+ SFWGNPNL++GSWMS P Y+A+FGWGR
Sbjct: 361 TGEYAQSAVDYLAGQDDFNRLRTSFHSKVPVQASFWGNPNLSIGSWMSFPFYEANFGWGR 420

Query: 421 PSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
           PSY  PA +N+DGK+YIM GP+NDGS+I+AIRLQT+HMDDFKK+FYED+
Sbjct: 421 PSYFAPATLNTDGKSYIMVGPSNDGSLIVAIRLQTRHMDDFKKYFYEDI 460

BLAST of MC01g_new0244 vs. NCBI nr
Match: XP_022949717.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata])

HSP 1 Score: 660 bits (1702), Expect = 1.32e-234
Identity = 324/468 (69.23%), Postives = 387/468 (82.69%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MASLTI+SSTTVFP+HPTP A LS SE+DQ + WTH PTVY+Y+SP    T+AV+ SL+N
Sbjct: 1   MASLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPTVT-TSAVVESLRN 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQ+LVPYYPLAGRL  I GGRLEL CC+AGA L+EASSTA LDDYGDF+P+DALR LA
Sbjct: 61  SLSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELA 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P VDY ++PIEE+P+ L+QVTRFSCGG+V+G   SH LVDG SAITFINSWAS+AR +KT
Sbjct: 121 PKVDY-STPIEELPVFLIQVTRFSCGGIVIGTGLSHILVDGVSAITFINSWASIARKDKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240
           V+SI QPFHDR  LQPQKP+ PPRF H EYD PP L G S+A+EE KKES+  LLKL+KE
Sbjct: 181 VESIVQPFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLKLSKE 240

Query: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300
           QVEKLKKRANSN  N          LD EL  RPY+RYESI G+MW+CACKAR+  +  Q
Sbjct: 241 QVEKLKKRANSNIVN---------QLD-ELPSRPYTRYESIAGHMWVCACKARSTGDKSQ 300

Query: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360
           PT+VR+ VD+ RRL TP+P NFTGNS L+ +TP+C+FGDL++QPLSYA GKIREGTWK+T
Sbjct: 301 PTMVRVAVDVRRRLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKIT 360

Query: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420
           DEYA+SA+DFLA +EDI+W RT +H+ V+V+  FWGNPNL+LGSWMS+PLY+ADFGWG+P
Sbjct: 361 DEYARSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGKP 420

Query: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
            YVGPA +N+DGK++IM  P NDG +IIAIRLQ KHMDDFK+ FYEDL
Sbjct: 421 CYVGPATLNADGKSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYEDL 456

BLAST of MC01g_new0244 vs. ExPASy TrEMBL
Match: A0A6J1DRJ9 (spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=LOC111023179 PE=3 SV=1)

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN
Sbjct: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA
Sbjct: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT
Sbjct: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240
           VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE
Sbjct: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240

Query: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300
           QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ
Sbjct: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300

Query: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360
           PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT
Sbjct: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360

Query: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420
           DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP
Sbjct: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420

Query: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
           SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL
Sbjct: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468

BLAST of MC01g_new0244 vs. ExPASy TrEMBL
Match: A0A6J1GCX6 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111453028 PE=3 SV=1)

HSP 1 Score: 660 bits (1702), Expect = 6.40e-235
Identity = 324/468 (69.23%), Postives = 387/468 (82.69%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MASLTI+SSTTVFP+HPTP A LS SE+DQ + WTH PTVY+Y+SP    T+AV+ SL+N
Sbjct: 1   MASLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPTVT-TSAVVESLRN 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQ+LVPYYPLAGRL  I GGRLEL CC+AGA L+EASSTA LDDYGDF+P+DALR LA
Sbjct: 61  SLSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELA 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P VDY ++PIEE+P+ L+QVTRFSCGG+V+G   SH LVDG SAITFINSWAS+AR +KT
Sbjct: 121 PKVDY-STPIEELPVFLIQVTRFSCGGIVIGTGLSHILVDGVSAITFINSWASIARKDKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240
           V+SI QPFHDR  LQPQKP+ PPRF H EYD PP L G S+A+EE KKES+  LLKL+KE
Sbjct: 181 VESIVQPFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLKLSKE 240

Query: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300
           QVEKLKKRANSN  N          LD EL  RPY+RYESI G+MW+CACKAR+  +  Q
Sbjct: 241 QVEKLKKRANSNIVN---------QLD-ELPSRPYTRYESIAGHMWVCACKARSTGDKSQ 300

Query: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360
           PT+VR+ VD+ RRL TP+P NFTGNS L+ +TP+C+FGDL++QPLSYA GKIREGTWK+T
Sbjct: 301 PTMVRVAVDVRRRLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKIT 360

Query: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420
           DEYA+SA+DFLA +EDI+W RT +H+ V+V+  FWGNPNL+LGSWMS+PLY+ADFGWG+P
Sbjct: 361 DEYARSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGKP 420

Query: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
            YVGPA +N+DGK++IM  P NDG +IIAIRLQ KHMDDFK+ FYEDL
Sbjct: 421 CYVGPATLNADGKSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYEDL 456

BLAST of MC01g_new0244 vs. ExPASy TrEMBL
Match: A0A6J1HTD7 (spermidine hydroxycinnamoyl transferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466005 PE=3 SV=1)

HSP 1 Score: 659 bits (1700), Expect = 1.49e-234
Identity = 321/469 (68.44%), Postives = 391/469 (83.37%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MAS+TI+SS+TV PSHPTP    S SE DQ+RAWTH+PT+Y+YKSPA    A  + SLK 
Sbjct: 1   MASITIHSSSTVVPSHPTPTGTFSFSECDQYRAWTHLPTIYVYKSPATEVVA--VESLKT 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQILVPYYPLAGRL  + GGRLELDCCAAG   +EAS+ AKL+DYGDFAPSDA+R+L 
Sbjct: 61  SLSQILVPYYPLAGRLHWLPGGRLELDCCAAGVLFVEASTDAKLEDYGDFAPSDAVRQLI 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P VDY NSPIEE+P+LL QVTRFSCGG+V+GV   HT+VDGASAITFINSWASMARGEKT
Sbjct: 121 PTVDY-NSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAITFINSWASMARGEKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPP-LLHGYSNAEEEEKKESVCALLKLTK 240
           V++I QP HDRKFL P+KP+ PPRF H E+ +PP ++ G SN EEE K+E+  ALLKLTK
Sbjct: 181 VETILQPLHDRKFLMPEKPLRPPRFDHIEFTKPPPVILGSSNPEEEAKQETTSALLKLTK 240

Query: 241 EQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESR 300
           EQ+EKLKK+ANSN      TNQ M + D E+QPRPYSR+E+ITG+MW+CACKARN  ESR
Sbjct: 241 EQIEKLKKKANSN-----LTNQTMGVAD-EVQPRPYSRFEAITGHMWVCACKARNTGESR 300

Query: 301 QPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKM 360
           QPTV+RI +D+ RR++ PIPENF+GNS  + +TP+C+FG+LM+QPLSYA GK+REGTWKM
Sbjct: 301 QPTVIRIPIDVRRRMQPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKM 360

Query: 361 TDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGR 420
           T EYA+S +D+LA ++DI+W RT  HS V VK SFWGNPNL++GSW+S+P Y+ADFGWGR
Sbjct: 361 TGEYAQSVVDYLAGQDDISWLRTSFHSKVLVKASFWGNPNLSIGSWISLPFYEADFGWGR 420

Query: 421 PSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
           PSY GPA +N DGK+YIM GP++DGS+I+AIRLQ +HMD FKK+FYED+
Sbjct: 421 PSYFGPATLNIDGKSYIMVGPSDDGSLIVAIRLQARHMDGFKKYFYEDI 460

BLAST of MC01g_new0244 vs. ExPASy TrEMBL
Match: A0A6J1EB80 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111432535 PE=3 SV=1)

HSP 1 Score: 659 bits (1699), Expect = 2.12e-234
Identity = 325/469 (69.30%), Postives = 392/469 (83.58%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MAS+TI+SS+TV PSHPTP  A S+SE DQ+RAWTH+P+VY+YKSPAA     ++ SLK 
Sbjct: 1   MASITIHSSSTVIPSHPTPTGAFSVSECDQYRAWTHLPSVYVYKSPAAEVI--IVESLKT 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQILVP+YPLAGRL  + GGRLELDCCAAGA  +EAS+ AKL+DYGDFAPSDA+R L 
Sbjct: 61  SLSQILVPFYPLAGRLHWLPGGRLELDCCAAGALFVEASTNAKLEDYGDFAPSDAVRELV 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P+VDY NSPIEE+P+LL QVTRFSCGG+V+GV   HT+VDGASA+TFINSWASMARGEKT
Sbjct: 121 PSVDY-NSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAVTFINSWASMARGEKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPP-LLHGYSNAEEEEKKESVCALLKLTK 240
           V++I QP HDRKFL P+KP+ PPRF H E+ +PP L+ G SN EEE KKE+  ALLKLTK
Sbjct: 181 VETILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPLILGSSNTEEEAKKETTSALLKLTK 240

Query: 241 EQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESR 300
           EQ+EKLKKRANSN      TNQ M + D E+QPRPYSR+E+ITG+MW+CACKARN  ESR
Sbjct: 241 EQIEKLKKRANSN-----LTNQTMDVAD-EVQPRPYSRFEAITGHMWVCACKARNTGESR 300

Query: 301 QPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKM 360
           QPTV+RI +D+ RR++ PIPENF+GNS  +  TP+C+FG+LM+QPLSYA GKIREGTWKM
Sbjct: 301 QPTVIRIPIDVRRRMKPPIPENFSGNSSFVVSTPECEFGELMSQPLSYAAGKIREGTWKM 360

Query: 361 TDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGR 420
           T EYA+SA+D+LA ++D    RT  HS V V+ SFWGNPNL++GSWMS P Y+ADFGWGR
Sbjct: 361 TGEYAQSAVDYLAGQDDFNRLRTSFHSKVPVQASFWGNPNLSIGSWMSFPFYEADFGWGR 420

Query: 421 PSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
           PSY  PA +N+DGK+YIM GP++DGS+IIAIRLQT+HMDDFKK+ YED+
Sbjct: 421 PSYFTPATLNTDGKSYIMVGPSDDGSLIIAIRLQTRHMDDFKKYLYEDI 460

BLAST of MC01g_new0244 vs. ExPASy TrEMBL
Match: A0A6J1EEM2 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111432536 PE=3 SV=1)

HSP 1 Score: 657 bits (1695), Expect = 8.29e-234
Identity = 323/469 (68.87%), Postives = 393/469 (83.80%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MAS+TI+SS+TV PSHPTP  A S SE DQ+RAWTH+P+VY+YKSPAA   A  + SLK 
Sbjct: 1   MASITIHSSSTVVPSHPTPTGAFSFSECDQYRAWTHLPSVYVYKSPAAEVVA--VESLKT 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLSQILVPYYPLAGRL  + GGRLELDCCAAGA  +EAS+ AKL+DYGDFAPSDA+R L 
Sbjct: 61  SLSQILVPYYPLAGRLHWLPGGRLELDCCAAGALFVEASTDAKLEDYGDFAPSDAVRELV 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P+VDY NSPIEE+P+LL QVTRF CGG+V+GV   HT+VDGASAITFINSWASMARGEKT
Sbjct: 121 PSVDY-NSPIEELPLLLFQVTRFRCGGVVIGVANCHTVVDGASAITFINSWASMARGEKT 180

Query: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPP-LLHGYSNAEEEEKKESVCALLKLTK 240
           V++I QP HDRKFL P+KP+ PPRF H E+ +PP ++ G SN+EE  K+E+  ALLKLTK
Sbjct: 181 VETILQPLHDRKFLMPEKPLRPPRFDHVEFTKPPPVILGTSNSEEA-KQETTSALLKLTK 240

Query: 241 EQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESR 300
           EQ+EKLKKRANSN      TN+ M + D E+QPRPYSR+E+ITG+MW+CACKARN  ESR
Sbjct: 241 EQIEKLKKRANSN-----LTNKTMGVGD-EVQPRPYSRFEAITGHMWVCACKARNTGESR 300

Query: 301 QPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKM 360
           QPT+VR  +D+ RR++ PIPENF+GNS  + +TP+C+FG+LM+QPLSYA GK+REGTWKM
Sbjct: 301 QPTIVRTPIDVRRRMKPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKM 360

Query: 361 TDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGR 420
           T EYA+S +D+LA ++DI W RT  HS V V+ SFWGNPNL++GSWMS+P Y+ADFGWGR
Sbjct: 361 TGEYAQSVVDYLAGQDDINWLRTSFHSKVPVQASFWGNPNLSIGSWMSLPFYEADFGWGR 420

Query: 421 PSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468
           PSY GPA +N DGK+YIM GP++DGS+I+AIRLQT+HMDDFKK+FYED+
Sbjct: 421 PSYFGPATLNIDGKSYIMVGPSDDGSLIVAIRLQTRHMDDFKKYFYEDI 459

BLAST of MC01g_new0244 vs. TAIR 10
Match: AT2G19070.1 (spermidine hydroxycinnamoyl transferase )

HSP 1 Score: 354.0 bits (907), Expect = 1.9e-97
Identity = 185/472 (39.19%), Postives = 283/472 (59.96%), Query Frame = 0

Query: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60
           MA +T   S T+ P+ PT +    L+E DQ    TH+PT+Y Y  P+ +    V+  LK 
Sbjct: 1   MAPITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNVVEILKT 60

Query: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120
           SLS++LV +YP+AGRLR +  GR EL+C A G   +EA S  KL D+ DF+P+     L 
Sbjct: 61  SLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFSPTPEFENLM 120

Query: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180
           P V+Y N PIE +P+ L QVT+F CGG+ + V  SH +VDG SA+  I+ W  +ARGE  
Sbjct: 121 PQVNYKN-PIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGE-P 180

Query: 181 VDSIPQPFHDRKFLQPQKPVP----PPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLK 240
           ++++  PF DRK L   +P+P    PP+F H E+D+PP L G ++  EE KK+++  +L 
Sbjct: 181 LETV--PFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLP 240

Query: 241 LTKEQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNA 300
           L+  Q++KL+ +AN ++              H    + ++RYE++TG++W CACKAR ++
Sbjct: 241 LSTSQLQKLRSKANGSK--------------HSDPAKGFTRYETVTGHVWRCACKARGHS 300

Query: 301 ESRQPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGT 360
              QPT + I +D   R+  P+P  + GN+ L  +      G+L++  L +A   I +  
Sbjct: 301 -PEQPTALGICIDTRSRMEPPLPRGYFGNATLDVVAASTS-GELISNELGFAASLISKAI 360

Query: 361 WKMTDEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFG 420
             +T+EY    +++L  ++D+   + L H++   +G F+GNPNL + SW+++P+Y  DFG
Sbjct: 361 KNVTNEYVMIGIEYLKNQKDLKKFQDL-HALGSTEGPFYGNPNLGVVSWLTLPMYGLDFG 420

Query: 421 WGRPSYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 469
           WG+  Y GP   + DG + I+     DGSVI+A  LQ  HM+ FKK FYED+
Sbjct: 421 WGKEFYTGPGTHDFDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYEDI 451

BLAST of MC01g_new0244 vs. TAIR 10
Match: AT5G48930.1 (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase )

HSP 1 Score: 285.4 bits (729), Expect = 8.2e-77
Identity = 172/464 (37.07%), Postives = 261/464 (56.25%), Query Frame = 0

Query: 4   LTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKNSLS 63
           + I  ST V P+  TP   L  S  D      H P+VY Y+ P  A+       +K +LS
Sbjct: 3   INIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYR-PTGASNFFDPQVMKEALS 62

Query: 64  QILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLAPNV 123
           + LVP+YP+AGRL+    GR+E+DC  AG   + A + + +DD+GDFAP+  LR+L P V
Sbjct: 63  KALVPFYPMAGRLKRDDDGRIEIDCNGAGVLFVVADTPSVIDDFGDFAPTLNLRQLIPEV 122

Query: 124 DYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKTVDS 183
           D+ ++ I   P+L++QVT F CGG  +GV   H   DG S + FIN+W+ MARG   +D 
Sbjct: 123 DH-SAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMARG---LDL 182

Query: 184 IPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKEQVE 243
              PF DR  L+ + P P P FHH EY   P +    +  +   + +  ++ KLT++Q+ 
Sbjct: 183 TIPPFIDRTLLRARDP-PQPAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLV 242

Query: 244 KLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQPTV 303
            LK +   ++ + NT +              YS YE + G++W    KAR    + Q T 
Sbjct: 243 ALKAK---SKEDGNTVS--------------YSSYEMLAGHVWRSVGKAR-GLPNDQETK 302

Query: 304 VRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMTDEY 363
           + I  D   RLR  +P  + GN  + T TP    GDL+++P  YA G+I +   +M D Y
Sbjct: 303 LYIATDGRSRLRPQLPPGYFGN-VIFTATPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNY 362

Query: 364 AKSAMDFLARKEDITWARTLHHSIVRVKGSF-WGNPNLALGSWMSMPLYDADFGWGRPSY 423
            +SA+D+L  + D++           V+G+  +  PNL + SW+ +P+YDADFGWGRP +
Sbjct: 363 LRSALDYLEMQPDLS---------ALVRGAHTYKCPNLGITSWVRLPIYDADFGWGRPIF 422

Query: 424 VGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYE 467
           +GP  +  +G ++++  PTNDGS+ +AI LQ++HM  F+KF +E
Sbjct: 423 MGPGGIPYEGLSFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of MC01g_new0244 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 179.5 bits (454), Expect = 6.3e-45
Identity = 139/458 (30.35%), Postives = 213/458 (46.51%), Query Frame = 0

Query: 12  VFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKNSLSQILVPYYP 71
           V P   T      LS  DQ  A   V T+Y +KS       AV   +K +LSQ+LV YYP
Sbjct: 41  VKPESETRKGLYFLSNLDQNIA-VIVRTIYCFKSEERGNEEAV-QVIKKALSQVLVHYYP 100

Query: 72  LAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSD--ALRRLAPNVDYDNSP 131
           LAGRL +   G+L +DC   G   +EA +  K+D+ GD    D   L +L  +V  D   
Sbjct: 101 LAGRLTISPEGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDV-VDAKN 160

Query: 132 IEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKTVDSIPQPFH 191
           I E+P +  QVT+F CGG V+G+  +H + DG  A+ F+NSW  +ARG   +     PF 
Sbjct: 161 ILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVARG---LPLTTPPFS 220

Query: 192 DRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKE-SVCALLKLTKEQVEKLKKR 251
           DR  L  + P P     H E++E   +   SN      KE ++        E+++KLK +
Sbjct: 221 DRTILNARNP-PKIENLHQEFEE---IEDKSNINSLYTKEPTLYRSFCFDPEKIKKLKLQ 280

Query: 252 ANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQPTVVRIIV 311
           A  N                 L     + +E+++ ++W    K+     S Q T +   V
Sbjct: 281 ATEN--------------SESLLGNSCTSFEALSAFVWRARTKSLKML-SDQKTKLLFAV 340

Query: 312 DISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMTDEYAKSAM 371
           D   +    +P+ + GN  ++T    C+ G+L+ +PLS+AVG +RE    +TD Y +SA+
Sbjct: 341 DGRAKFEPQLPKGYFGNGIVLT-NSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAI 400

Query: 372 DFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRPSYVGPAMV 431
           D+     ++T AR    S             L + +W  +  +  DFGWG P   GP  +
Sbjct: 401 DYF----EVTRARPSLSS------------TLLITTWSRLGFHTTDFGWGEPILSGPVAL 456

Query: 432 NSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYE 467
                   +S      S+ + + L    MD F++ F +
Sbjct: 461 PEKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 456

BLAST of MC01g_new0244 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 179.5 bits (454), Expect = 6.3e-45
Identity = 139/458 (30.35%), Postives = 213/458 (46.51%), Query Frame = 0

Query: 12  VFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKNSLSQILVPYYP 71
           V P   T      LS  DQ  A   V T+Y +KS       AV   +K +LSQ+LV YYP
Sbjct: 25  VKPESETRKGLYFLSNLDQNIA-VIVRTIYCFKSEERGNEEAV-QVIKKALSQVLVHYYP 84

Query: 72  LAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSD--ALRRLAPNVDYDNSP 131
           LAGRL +   G+L +DC   G   +EA +  K+D+ GD    D   L +L  +V  D   
Sbjct: 85  LAGRLTISPEGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPETLGKLVYDV-VDAKN 144

Query: 132 IEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKTVDSIPQPFH 191
           I E+P +  QVT+F CGG V+G+  +H + DG  A+ F+NSW  +ARG   +     PF 
Sbjct: 145 ILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQVARG---LPLTTPPFS 204

Query: 192 DRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKE-SVCALLKLTKEQVEKLKKR 251
           DR  L  + P P     H E++E   +   SN      KE ++        E+++KLK +
Sbjct: 205 DRTILNARNP-PKIENLHQEFEE---IEDKSNINSLYTKEPTLYRSFCFDPEKIKKLKLQ 264

Query: 252 ANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQPTVVRIIV 311
           A  N                 L     + +E+++ ++W    K+     S Q T +   V
Sbjct: 265 ATEN--------------SESLLGNSCTSFEALSAFVWRARTKSLKML-SDQKTKLLFAV 324

Query: 312 DISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMTDEYAKSAM 371
           D   +    +P+ + GN  ++T    C+ G+L+ +PLS+AVG +RE    +TD Y +SA+
Sbjct: 325 DGRAKFEPQLPKGYFGNGIVLT-NSICEAGELIEKPLSFAVGLVREAIKMVTDGYMRSAI 384

Query: 372 DFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRPSYVGPAMV 431
           D+     ++T AR    S             L + +W  +  +  DFGWG P   GP  +
Sbjct: 385 DYF----EVTRARPSLSS------------TLLITTWSRLGFHTTDFGWGEPILSGPVAL 440

Query: 432 NSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYE 467
                   +S      S+ + + L    MD F++ F +
Sbjct: 445 PEKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 440

BLAST of MC01g_new0244 vs. TAIR 10
Match: AT3G48720.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.7e-42
Identity = 138/456 (30.26%), Postives = 215/456 (47.15%), Query Frame = 0

Query: 14  PSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKNSLSQILVPYYPLA 73
           P   TP     LS  DQ  A   V T+Y YKS +     +    +K SLS++LV YYP+A
Sbjct: 19  PVSETPNGHYYLSNLDQNIA-IIVKTLYYYKSESRTNQES-YNVIKKSLSEVLVHYYPVA 78

Query: 74  GRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLAPNVDYD---NSPI 133
           GRL +   G++ ++C   G  ++EA +   +D   +    + +  L   V YD      I
Sbjct: 79  GRLTISPEGKIAVNCTGEGVVVVEAEANCGIDTIKEAISENRMETLEKLV-YDVPGARNI 138

Query: 134 EEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKTVDSIPQPFHD 193
            E+P ++VQVT F CGG V+G+  SH + DG +A  F+NSW  MA+G     S+P PF D
Sbjct: 139 LEIPPVVVQVTNFKCGGFVLGLGMSHNMFDGVAAAEFLNSWCEMAKGLPL--SVP-PFLD 198

Query: 194 RKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKEQVEKLKKRAN 253
           R  L+ + P P   F H E+DE   +       +EEK   +        E++EKLK  A 
Sbjct: 199 RTILRSRNP-PKIEFPHNEFDEIEDISDTGKIYDEEK--LIYKSFLFEPEKLEKLKIMAI 258

Query: 254 SNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQPTVVRIIVDI 313
               N                    S ++++TG++W   C+A    +  Q   +    D 
Sbjct: 259 EENNN-----------------NKVSTFQALTGFLWKSRCEAL-RFKPDQRVKLLFAADG 318

Query: 314 SRRLRTPIPENFTGNSCLIT--ITPQCKFGDLMTQPLSYAVGKIREGTWKMTDEYAKSAM 373
             R    +P+ + GN  ++T  +T     G+L+  PLS++VG ++     +TD + +SAM
Sbjct: 319 RSRFIPRLPQGYCGNGIVLTGLVTSS---GELVGNPLSHSVGLVKRLVELVTDGFMRSAM 378

Query: 374 DFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRPSYVGPAMV 433
           D+                + R + S   N  L + SW  + L+  DFGWG P + GP  V
Sbjct: 379 DYF--------------EVNRTRPSM--NATLLITSWSKLTLHKLDFGWGEPVFSGP--V 426

Query: 434 NSDGKAYIMSGPTNDG--SVIIAIRLQTKHMDDFKK 463
              G+  I+  P+ D   S+ + + L T  M+ F++
Sbjct: 439 GLPGREVILFLPSGDDMKSINVFLGLPTSAMEVFEE 426

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O644702.6e-9639.19Spermidine hydroxycinnamoyl transferase OS=Arabidopsis thaliana OX=3702 GN=SHT P... [more]
Q8GSM75.2e-7637.63Shikimate O-hydroxycinnamoyltransferase OS=Nicotiana tabacum OX=4097 GN=HST PE=1... [more]
Q9FI781.1e-7537.07Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST P... [more]
A0A2H5AIZ16.5e-7134.89Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV... [more]
Q0JBZ84.1e-6534.25Hydroxycinnamoyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=HCT1 P... [more]
Match NameE-valueIdentityDescription
XP_022156247.10.0100.00spermidine hydroxycinnamoyl transferase-like [Momordica charantia][more]
XP_023544822.13.84e-23669.16spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo][more]
KAG6603487.12.29e-23569.44Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. ... [more]
KAG6595485.13.77e-23569.30Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022949717.11.32e-23469.23spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1DRJ90.0100.00spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1GCX66.40e-23569.23spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1HTD71.49e-23468.44spermidine hydroxycinnamoyl transferase-like isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A6J1EB802.12e-23469.30spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1EEM28.29e-23468.87spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT2G19070.11.9e-9739.19spermidine hydroxycinnamoyl transferase [more]
AT5G48930.18.2e-7737.07hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase [more]
AT5G41040.16.3e-4530.35HXXXD-type acyl-transferase family protein [more]
AT5G41040.26.3e-4530.35HXXXD-type acyl-transferase family protein [more]
AT3G48720.11.7e-4230.26HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 3..466
e-value: 2.7E-101
score: 339.4
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 3..212
e-value: 4.1E-61
score: 208.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 217..466
e-value: 1.4E-58
score: 199.7
NoneNo IPR availablePANTHERPTHR31642:SF57SPERMIDINE HYDROXYCINNAMOYL TRANSFERASEcoord: 7..467
NoneNo IPR availablePANTHERPTHR31642TRICHOTHECENE 3-O-ACETYLTRANSFERASEcoord: 7..467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g_new0244.1MC01g_new0244.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups