MC01g_new0242 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0242 vs. ExPASy Swiss-Prot
Match: O64470 (Spermidine hydroxycinnamoyl transferase OS=Arabidopsis thaliana OX=3702 GN=SHT PE=1 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 5.0e-84
Identity = 177/457 (38.73%), Postives = 262/457 (57.33%), Query Frame = 0

Query: 8   VTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNSL 67
           +T   S TI+P+ PT      L+E DQ     +I  +Y Y K  +    ++ ++ ++ SL
Sbjct: 4   ITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNV-VEILKTSL 63

Query: 68  SQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQLV 127
           S++LV +YP+AGRLRW+  GR E++C   GV+  EAE  ++ K  D+ DF P+     L+
Sbjct: 64  SRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAE--SEGKLSDFKDFSPTPEFENLM 123

Query: 128 PKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGE--K 187
           P+ +Y+ PIE  PL L QVT+F CGG+ + + VSH +VDG S +  I+ W  +ARGE  +
Sbjct: 124 PQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGEPLE 183

Query: 188 TVESIIPK--WPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALLKL 247
           TV  +  K  W       L P + P  F H+E++ PP LIG +   EER K+T V +L L
Sbjct: 184 TVPFLDRKILWAGEP---LPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLPL 243

Query: 248 TKHQHEKLKKIAN--EGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALITA 307
           +  Q +KL+  AN  + S   + +TR+E V GH+W CACKAR  +     +QPTA  I  
Sbjct: 244 STSQLQKLRSKANGSKHSDPAKGFTRYETVTGHVWRCACKARGHS----PEQPTALGICI 303

Query: 308 SIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSAI 367
             R R+ PP P G+ GNAT L V      G+LI N L +AA  I +    +T+EY+   I
Sbjct: 304 DTRSRMEPPLPRGYFGNAT-LDVVAASTSGELISNELGFAASLISKAIKNVTNEYVMIGI 363

Query: 368 SFLAS-REIEWIKEWYRTKAT--PFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLV 427
            +L + ++++  ++ +   +T  PF+GNPNL +  W  +P+Y  DFGWG+  Y GP    
Sbjct: 364 EYLKNQKDLKKFQDLHALGSTEGPFYGNPNLGVVSWLTLPMYGLDFGWGKEFYTGPGTHD 423

Query: 428 VDDGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQ 456
            D    I+    +DGS+ +  CLQ  HM+ FKK FY+
Sbjct: 424 FDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYE 449

BLAST of MC01g_new0242 vs. ExPASy Swiss-Prot
Match: Q9FI78 (Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.0e-68
Identity = 162/455 (35.60%), Postives = 247/455 (54.29%), Query Frame = 0

Query: 8   VTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDS--MRN 67
           + +  S+ + P+  TP   L  S  D  +   +   +Y Y+       +S   D   M+ 
Sbjct: 3   INIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYRPT----GASNFFDPQVMKE 62

Query: 68  SLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQ 127
           +LS+ LVP+YP+AGRL+  D GR+EIDC GAGV      A   +  DD+GDF P+   RQ
Sbjct: 63  ALSKALVPFYPMAGRLKRDDDGRIEIDCNGAGVLF--VVADTPSVIDDFGDFAPTLNLRQ 122

Query: 128 LVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARG-E 187
           L+P+ D+ A I   PL+++QVT F CGG  +G+ + H   DG S + FIN+W+ +ARG +
Sbjct: 123 LIPEVDHSAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMARGLD 182

Query: 188 KTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALLKLT 247
            T+   I +     RD  +P      FHH EY+  P +       +   + TTV++ KLT
Sbjct: 183 LTIPPFIDRTLLRARDPPQPA-----FHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLT 242

Query: 248 KHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALITASIR 307
           + Q   LK  + E  + +  Y+ +E + GH+W    KAR       +DQ T   I    R
Sbjct: 243 RDQLVALKAKSKEDGNTV-SYSSYEMLAGHVWRSVGKARG----LPNDQETKLYIATDGR 302

Query: 308 QRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSAISFL 367
            R+RP  P G+ GN  + T TP+   GDL+  P  YAAG+I +   ++ D Y++SA+ +L
Sbjct: 303 SRLRPQLPPGYFGN-VIFTATPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNYLRSALDYL 362

Query: 368 ASR-EIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGP--IPLVVDD 427
             + ++  +     T     +  PNL +  W  +P+YDADFGWGRP ++GP  IP    +
Sbjct: 363 EMQPDLSALVRGAHT-----YKCPNLGITSWVRLPIYDADFGWGRPIFMGPGGIPY---E 422

Query: 428 GWSIIMTGP-DDGSLAVVICLQTKHMDEFKKLFYQ 456
           G S ++  P +DGSL+V I LQ++HM  F+K  ++
Sbjct: 423 GLSFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of MC01g_new0242 vs. ExPASy Swiss-Prot
Match: Q8GSM7 (Shikimate O-hydroxycinnamoyltransferase OS=Nicotiana tabacum OX=4097 GN=HST PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.9e-67
Identity = 158/453 (34.88%), Postives = 245/453 (54.08%), Query Frame = 0

Query: 8   VTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNSL 67
           + +  S+ + P+  TP + L  S  D  +   +   +Y Y+     +     +  ++ +L
Sbjct: 3   IEVKESTMVKPAAETPQQRLWNSNVDLVVPNFHTPSVYFYRPTGSPNFFDGKV--LKEAL 62

Query: 68  SQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQLV 127
           S+ LVP+YP+AGRL   + GR+EIDC G GV   EAE  +D   DD+GDF P+   RQL+
Sbjct: 63  SKALVPFYPMAGRLCRDEDGRIEIDCKGQGVLFVEAE--SDGVVDDFGDFAPTLELRQLI 122

Query: 128 PKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARG-EKT 187
           P  DY   I+   L+++Q+T F CGGV +G+ + H   DG S + FIN+W+ +ARG + T
Sbjct: 123 PAVDYSQGIQSYALLVLQITHFKCGGVSLGVGMQHHAADGASGLHFINTWSDMARGLDLT 182

Query: 188 VESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLL--IGHSCVEEERNKQTTVALLKLT 247
           +   I +     RD  +P      F H EY+ PP L     +    E   +T+V++ KLT
Sbjct: 183 IPPFIDRTLLRARDPPQP-----QFPHVEYQPPPTLKVTPENTPISEAVPETSVSIFKLT 242

Query: 248 KHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALITASIR 307
           + Q   LK  + E  + +  Y+ +E + GH+W   C AR        DQ T   I    R
Sbjct: 243 RDQINTLKAKSKEDGNTVN-YSSYEMLAGHVWRSTCMARG----LAHDQETKLYIATDGR 302

Query: 308 QRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSAISFL 367
            R+RP  P G+ GN  + T TPI   GD+   P+ YAA K+ +   ++ ++Y++SA+ +L
Sbjct: 303 SRLRPSLPPGYFGN-VIFTTTPIAVAGDIQSKPIWYAASKLHDALARMDNDYLRSALDYL 362

Query: 368 ASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVDDGWS 427
             +    +K   R   T  +  PNL +  W+ +P++DADFGWGRP ++GP  +   +G S
Sbjct: 363 ELQPD--LKALVRGAHT--FKCPNLGITSWSRLPIHDADFGWGRPIFMGPGGIAY-EGLS 422

Query: 428 IIMTGP-DDGSLAVVICLQTKHMDEFKKLFYQY 457
            I+  P +DGS +V I LQ +HM  F+K  Y +
Sbjct: 423 FILPSPTNDGSQSVAISLQAEHMKLFEKFLYDF 435

BLAST of MC01g_new0242 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ1 (Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.4e-64
Identity = 153/456 (33.55%), Postives = 247/456 (54.17%), Query Frame = 0

Query: 8   VTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNSL 67
           + +  ++ + PS PTP + L  S  D  +   +   +Y Y++  +  +       ++ +L
Sbjct: 3   INIKETTMVRPSQPTPSQRLWNSNLDLVVPRFHTPSVYFYRRPGNA-SDFFDARVLKEAL 62

Query: 68  SQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQLV 127
            + LVP+YP+AGRL   + GR+EIDC G GV+   AE   D+  D++GDF P+   ++L+
Sbjct: 63  GRALVPFYPMAGRLARDEDGRVEIDCNGEGVRFVVAE--TDSAIDEFGDFAPTMELKKLI 122

Query: 128 PKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGEKTV 187
           PK +Y   I   PL+++Q+T F CGG  +G+ + H V DG S + FINSW+ IARG   +
Sbjct: 123 PKVEYGDDISAFPLLVLQITHFKCGGTSLGVGMQHHVADGASGLHFINSWSDIARG---L 182

Query: 188 ESIIPKWPNHERDFLRPVLDPL-HFHHQEYE-SPPLLIGHSCVEEERNKQ----TTVALL 247
           +  +P  P  +R  LR    P   F H EY+ +P +    + +++   K     T V++ 
Sbjct: 183 DIAVP--PFIDRSLLRARDPPSPSFPHIEYQPAPSMNTSPAPIQDPTVKSDPTATAVSIF 242

Query: 248 KLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALITA 307
           KLTK Q + LK      S +   Y+ +  V GH+W C   AR        DQ T      
Sbjct: 243 KLTKQQLDLLK------SRVSAKYSSYALVAGHVWRCTSIARG----LPDDQRTKLYCAT 302

Query: 308 SIRQRIRPPPPEGFCGNATLLTVTPICKFGDLI--KNPLSYAAGKIREGTWKLTDEYIKS 367
             R R++PP P G+ GN  + T TP+   G++    + L  AAG+I+    ++ DEY++S
Sbjct: 303 DGRARLQPPLPSGYFGN-VIFTATPVADAGEITGEDSGLEAAAGRIQRALMRMDDEYLRS 362

Query: 368 AISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVV 427
           A+ +L  +    + +  R   T  +  PN+ L  WT +P++DADFGWGRP ++GP  +  
Sbjct: 363 ALDYLELQPD--LSKLVRGAHT--FRCPNIGLTSWTRLPIHDADFGWGRPIFMGPGGIAY 422

Query: 428 DDGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQ 456
           +    ++ +   DGSL++ I LQ +HM +F+KL Y+
Sbjct: 423 EGLAFMLPSSEGDGSLSIAISLQAEHMIKFQKLLYE 435

BLAST of MC01g_new0242 vs. ExPASy Swiss-Prot
Match: Q0JBZ8 (Hydroxycinnamoyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=HCT1 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.2e-63
Identity = 152/458 (33.19%), Postives = 241/458 (52.62%), Query Frame = 0

Query: 7   SVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFD---IDASSLTIDSM 66
           ++T+  S+ + P+  TP   L  S  D  +   +   +Y Y++  +           + M
Sbjct: 2   AITVRRSTMVRPAWETPRVRLWNSNLDLVVPRFHTPSVYFYRRGPEGGGAPEGFFDGERM 61

Query: 67  RNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDAT 126
           R +L++ LVP+YP+AGRL   + GR+EIDC G GV   EA+A  DA  DDYGDF P+   
Sbjct: 62  RRALAEALVPFYPMAGRLARDEDGRVEIDCNGEGVLFVEADA-PDASVDDYGDFAPTMEL 121

Query: 127 RQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARG 186
           ++L+P  DY   I    L+++QVT F CGGV +G+ + H V DG+S + FINSW+ + RG
Sbjct: 122 KRLIPAVDYTDDISSFSLLVLQVTYFKCGGVSLGVGMQHHVADGMSGLHFINSWSDLCRG 181

Query: 187 EKTVESIIPKWPNHERDFLRPVLDPL-HFHHQEYESPPLLIGHSCVEEERNK----QTTV 246
            +     I   P  +R  LR    P   + H EY+  P ++         NK     T V
Sbjct: 182 TQ-----IAIMPFIDRTLLRARDPPTPSYPHVEYQPAPAMLSSVPQSVTANKTTPPPTAV 241

Query: 247 ALLKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAAL 306
            + KLT+    +L+     G    R ++ +  +  H+W C   AR       S+QPT   
Sbjct: 242 DIFKLTRSDLGRLRSQLPSGEGAPR-FSTYAVLAAHVWRCVSLARG----LPSEQPTKLY 301

Query: 307 ITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIK 366
                RQR++PP PEG+ GN  + T TP+ + G  + + L+  A  I+E   ++ D Y +
Sbjct: 302 CATDGRQRLQPPLPEGYFGN-VIFTATPLAEAGK-VTSGLADGAAVIQEALDRMNDSYCR 361

Query: 367 SAISFLASR-EIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPL 426
           SA+ +L  + ++  +     T     +  PNL L  W  +P++DADFGWGRP ++GP  +
Sbjct: 362 SALDYLELQPDLSALVRGAHT-----FRCPNLGLTSWVRLPIHDADFGWGRPVFMGPGGI 421

Query: 427 VVDDGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQ 456
             +    ++ +   DGSL++ I LQ +HM++F+KL ++
Sbjct: 422 AYEGLAFVLPSANKDGSLSIAISLQAEHMEKFRKLIFE 441

BLAST of MC01g_new0242 vs. NCBI nr
Match: XP_022156263.1 (spermidine hydroxycinnamoyl transferase-like [Momordica charantia])

HSP 1 Score: 931 bits (2406), Expect = 0.0
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MAMASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTI 60
           MAMASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTI
Sbjct: 1   MAMASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTI 60

Query: 61  DSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPS 120
           DSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPS
Sbjct: 61  DSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPS 120

Query: 121 DATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASI 180
           DATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASI
Sbjct: 121 DATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASI 180

Query: 181 ARGEKTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVAL 240
           ARGEKTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVAL
Sbjct: 181 ARGEKTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVAL 240

Query: 241 LKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALIT 300
           LKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALIT
Sbjct: 241 LKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALIT 300

Query: 301 ASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSA 360
           ASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSA
Sbjct: 301 ASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSA 360

Query: 361 ISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVD 420
           ISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVD
Sbjct: 361 ISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVD 420

Query: 421 DGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL 457
           DGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL
Sbjct: 421 DGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL 457

BLAST of MC01g_new0242 vs. NCBI nr
Match: XP_022156211.1 (spermidine hydroxycinnamoyl transferase-like [Momordica charantia])

HSP 1 Score: 785 bits (2026), Expect = 4.27e-284
Identity = 385/457 (84.25%), Postives = 417/457 (91.25%), Query Frame = 0

Query: 3   MASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFD-IDASSLTID 62
           MA PSVT+HSSSTIIPSHPTPHRTL LSESDQAML+GY F IYVYKKAFD +DASSL +D
Sbjct: 1   MAFPSVTIHSSSTIIPSHPTPHRTLALSESDQAMLDGYSFTIYVYKKAFDHLDASSLAMD 60

Query: 63  SMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSD 122
           SMRNSLS+ILVPYYPLAGRLRWIDGGRLE+DCCGAGV+  EAEA ADAK DDYGDF PSD
Sbjct: 61  SMRNSLSKILVPYYPLAGRLRWIDGGRLELDCCGAGVRFIEAEAFADAKLDDYGDFAPSD 120

Query: 123 ATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIA 182
           ATRQLVPKPD+RAPIEDQPLVLVQVTRFSCGG+VIG A+SHFVVDGIS I F+N WASI 
Sbjct: 121 ATRQLVPKPDHRAPIEDQPLVLVQVTRFSCGGIVIGTAISHFVVDGISAIMFVNLWASIT 180

Query: 183 RGEKTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALL 242
           +GEKT+ESIIPKWPNHERDFL+PVLDP  FHH+EYESPPLLIG S V+EER+K+TTVALL
Sbjct: 181 QGEKTIESIIPKWPNHERDFLQPVLDPPRFHHREYESPPLLIGRSDVKEERSKETTVALL 240

Query: 243 KLTKHQHEKLKKIANEGSSM-LRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALIT 302
           KL KHQHEKLKK+ANEGS+   RPYTRFEAVGGHIWAC CKAR++TT CESDQPT ALIT
Sbjct: 241 KLAKHQHEKLKKMANEGSATPQRPYTRFEAVGGHIWACVCKARSNTTRCESDQPTVALIT 300

Query: 303 ASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSA 362
           ASIRQRIRPPPPE FCGNA LLTVTPICKFGDLIKNPLSYAAG IREGTWKLTDEYIKSA
Sbjct: 301 ASIRQRIRPPPPERFCGNAILLTVTPICKFGDLIKNPLSYAAGNIREGTWKLTDEYIKSA 360

Query: 363 ISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVD 422
           ISFLAS+E E +KE Y+T+ TPFWGNPNLDLGCWT MP+YDADFGWGRPSYVGP PLVV+
Sbjct: 361 ISFLASKENELVKECYQTEVTPFWGNPNLDLGCWTSMPIYDADFGWGRPSYVGPTPLVVE 420

Query: 423 DGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL 457
           DG SIIM GPDDGSL ++ICLQTK M+EFKKLFYQ+L
Sbjct: 421 DGLSIIMPGPDDGSLILIICLQTKCMNEFKKLFYQHL 457

BLAST of MC01g_new0242 vs. NCBI nr
Match: XP_023544822.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 509 bits (1311), Expect = 2.14e-175
Identity = 266/462 (57.58%), Postives = 329/462 (71.21%), Query Frame = 0

Query: 7   SVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNS 66
           S+T+HSS+T+ P+HPTP  TL+ SESDQ     +   +YVY+      A+S  ++S+RNS
Sbjct: 3   SLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPAV--ATSTVVESLRNS 62

Query: 67  LSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQL 126
           LSQ+LVPYYPLAGRL WI GGRLE+ CC AG QL   EAS+ A  DDYGDF P+DA R+L
Sbjct: 63  LSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQL--IEASSTATLDDYGDFSPTDALREL 122

Query: 127 VPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGEKT 186
            PK DY  PIE+ P++LVQ+TRFSCGG+VIGI +SH +VDG+S ITFINSWASIAR EKT
Sbjct: 123 APKVDYSTPIEELPVLLVQITRFSCGGIVIGIGISHILVDGVSAITFINSWASIARKEKT 182

Query: 187 VESIIPKWPNHERDFLRPV--LDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALLKLT 246
           VESI+   P H+R  L+P   L P  F H EY++PP L+G    +EER K++TV LLKL+
Sbjct: 183 VESIVQ--PFHDRSILQPQKPLRPPRFDHGEYDNPPFLLGCLDAKEERKKESTVTLLKLS 242

Query: 247 KHQHEKLKKIANEGSSML------RPYTRFEAVGGHIWACACKARNSTTTCESDQPTAAL 306
           K Q EKLKK AN  +         RPYTR+E+V GH+W CACKARN+    ++ QPT   
Sbjct: 243 KEQVEKLKKRANSNTVTQLDEVPSRPYTRYESVAGHMWVCACKARNTN---DNSQPTMVR 302

Query: 307 ITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIK 366
           +   +R+R+R P P  F GN+TL+ VTP C+F DLI  PLSYAAGKIREGTWK+TDEY +
Sbjct: 303 VAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFNDLISQPLSYAAGKIREGTWKITDEYAR 362

Query: 367 SAISFLASRE-IEWIKEWYRTKA---TPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGP 426
           SA+ FLAS+E I W++  Y TK     PFWGNPNL LG W  +PLY+ADFGWG P YVGP
Sbjct: 363 SALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGPPCYVGP 422

Query: 427 IPLVVDDGWSIIMTGPD-DGSLAVVICLQTKHMDEFKKLFYQ 455
             L  D G S IM  PD DG L + + LQ KHMD+FK+LFY+
Sbjct: 423 ATLNAD-GKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 454

BLAST of MC01g_new0242 vs. NCBI nr
Match: KAG6603488.1 (Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 507 bits (1305), Expect = 2.38e-174
Identity = 265/461 (57.48%), Postives = 327/461 (70.93%), Query Frame = 0

Query: 8   VTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNSL 67
           +T+HSS+T+ P+HPTP  TL+ SESDQ     +   +YVY+       +S  +DS+RNSL
Sbjct: 13  LTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPAV--PTSAVVDSLRNSL 72

Query: 68  SQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQLV 127
           SQILVPYYPL GRL WI GGRLE+ CC AG QL   EAS+ A  DDYGDF P+DA R+L 
Sbjct: 73  SQILVPYYPLVGRLHWIGGGRLELHCCSAGAQL--IEASSTATLDDYGDFSPTDALRKLA 132

Query: 128 PKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGEKTV 187
           PK DY  PIE+ P++LVQ+TRFSCGG+VIGI +SH +VDG+S ITFINSWASIAR EKTV
Sbjct: 133 PKVDYSTPIEELPVLLVQITRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTV 192

Query: 188 ESIIPKWPNHERDFLRPV--LDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALLKLTK 247
           ESI+   P H+R  L+P   L P  F H EY +PPLL+G S  +EER K++TV LLKL+K
Sbjct: 193 ESIVK--PFHDRSVLQPQKPLRPPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSK 252

Query: 248 HQHEKLKKIANEGSSML------RPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALI 307
            Q EKLKK AN  +         RPYTR+E+V GH+W CACKAR++    ++ QPT   +
Sbjct: 253 EQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTN---DNSQPTMVRV 312

Query: 308 TASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKS 367
              +R+R+R P P  F GN+TL+ VTP C+F DL+  PLSYAAGKIREGTWK+TDEY +S
Sbjct: 313 AVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARS 372

Query: 368 AISFLASRE-IEWIKEWYRTKA---TPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPI 427
           A+ FLAS+E I W++  Y TK     PFWGNPNL LG W  +PLY+ADFGWG P YVGP 
Sbjct: 373 ALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGPPCYVGPA 432

Query: 428 PLVVDDGWSIIMTGPD-DGSLAVVICLQTKHMDEFKKLFYQ 455
            L  D G S IM  PD DG L + + LQ KHMD+FK+LFY+
Sbjct: 433 TLNAD-GKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 463

BLAST of MC01g_new0242 vs. NCBI nr
Match: XP_022950556.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata])

HSP 1 Score: 503 bits (1295), Expect = 5.72e-173
Identity = 266/463 (57.45%), Postives = 328/463 (70.84%), Query Frame = 0

Query: 7   SVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNS 66
           S+T+HSS+T+ P+HPTP  TL+ SESDQ     +   IYVY+       +S  +DS++NS
Sbjct: 3   SLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAV--PTSAVVDSLKNS 62

Query: 67  LSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQL 126
           LSQILVPYYPL GRL WI GGRLE+ CC AG QL   EAS+ A  DDYGDF P+DA R+L
Sbjct: 63  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQL--IEASSTATLDDYGDFSPTDALREL 122

Query: 127 VPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGEKT 186
            PK DY  PIE+ P++LVQ+TRFSCGG+VIGI +SH +VDG+S ITFINSWASIAR EKT
Sbjct: 123 APKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKT 182

Query: 187 VESIIPKWPNHERDFLRPVLDPLH---FHHQEYESPPLLIGHSCVEEERNKQTTVALLKL 246
           VESI+   P H+R  L+P   PL    F H EY +PPLL+G S  +EER K++TV LLKL
Sbjct: 183 VESIVQ--PFHDRRILQPQ-KPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKL 242

Query: 247 TKHQHEKLKKIANEGSSML------RPYTRFEAVGGHIWACACKARNSTTTCESDQPTAA 306
           +K Q EKLKK AN  +         RPYTR+E+V GH+W CACKAR++    ++ QPT  
Sbjct: 243 SKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTN---DNSQPTMV 302

Query: 307 LITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYI 366
            +   +R+R+R P P  F GN+TL+ VTP C+F DL+  PLSYAAGKIREGTWK+TDEY 
Sbjct: 303 RVAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYA 362

Query: 367 KSAISFLASRE-IEWIKEWYRTKA---TPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVG 426
           +SA+ FLAS+E I W++  Y TK     PFWGNPNL LG W  +PLY+ADFGWG P YVG
Sbjct: 363 RSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVG 422

Query: 427 PIPLVVDDGWSIIMTGPD-DGSLAVVICLQTKHMDEFKKLFYQ 455
           P  L  D G S IM  PD DG L + + LQ KHMD+FK+LFY+
Sbjct: 423 PATLNAD-GKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 454

BLAST of MC01g_new0242 vs. ExPASy TrEMBL
Match: A0A6J1DQ54 (spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=LOC111023192 PE=3 SV=1)

HSP 1 Score: 931 bits (2406), Expect = 0.0
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MAMASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTI 60
           MAMASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTI
Sbjct: 1   MAMASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTI 60

Query: 61  DSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPS 120
           DSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPS
Sbjct: 61  DSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPS 120

Query: 121 DATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASI 180
           DATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASI
Sbjct: 121 DATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASI 180

Query: 181 ARGEKTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVAL 240
           ARGEKTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVAL
Sbjct: 181 ARGEKTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVAL 240

Query: 241 LKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALIT 300
           LKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALIT
Sbjct: 241 LKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALIT 300

Query: 301 ASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSA 360
           ASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSA
Sbjct: 301 ASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSA 360

Query: 361 ISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVD 420
           ISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVD
Sbjct: 361 ISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVD 420

Query: 421 DGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL 457
           DGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL
Sbjct: 421 DGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL 457

BLAST of MC01g_new0242 vs. ExPASy TrEMBL
Match: A0A6J1DU82 (spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=LOC111023154 PE=3 SV=1)

HSP 1 Score: 785 bits (2026), Expect = 2.07e-284
Identity = 385/457 (84.25%), Postives = 417/457 (91.25%), Query Frame = 0

Query: 3   MASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFD-IDASSLTID 62
           MA PSVT+HSSSTIIPSHPTPHRTL LSESDQAML+GY F IYVYKKAFD +DASSL +D
Sbjct: 1   MAFPSVTIHSSSTIIPSHPTPHRTLALSESDQAMLDGYSFTIYVYKKAFDHLDASSLAMD 60

Query: 63  SMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSD 122
           SMRNSLS+ILVPYYPLAGRLRWIDGGRLE+DCCGAGV+  EAEA ADAK DDYGDF PSD
Sbjct: 61  SMRNSLSKILVPYYPLAGRLRWIDGGRLELDCCGAGVRFIEAEAFADAKLDDYGDFAPSD 120

Query: 123 ATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIA 182
           ATRQLVPKPD+RAPIEDQPLVLVQVTRFSCGG+VIG A+SHFVVDGIS I F+N WASI 
Sbjct: 121 ATRQLVPKPDHRAPIEDQPLVLVQVTRFSCGGIVIGTAISHFVVDGISAIMFVNLWASIT 180

Query: 183 RGEKTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALL 242
           +GEKT+ESIIPKWPNHERDFL+PVLDP  FHH+EYESPPLLIG S V+EER+K+TTVALL
Sbjct: 181 QGEKTIESIIPKWPNHERDFLQPVLDPPRFHHREYESPPLLIGRSDVKEERSKETTVALL 240

Query: 243 KLTKHQHEKLKKIANEGSSM-LRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALIT 302
           KL KHQHEKLKK+ANEGS+   RPYTRFEAVGGHIWAC CKAR++TT CESDQPT ALIT
Sbjct: 241 KLAKHQHEKLKKMANEGSATPQRPYTRFEAVGGHIWACVCKARSNTTRCESDQPTVALIT 300

Query: 303 ASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSA 362
           ASIRQRIRPPPPE FCGNA LLTVTPICKFGDLIKNPLSYAAG IREGTWKLTDEYIKSA
Sbjct: 301 ASIRQRIRPPPPERFCGNAILLTVTPICKFGDLIKNPLSYAAGNIREGTWKLTDEYIKSA 360

Query: 363 ISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVD 422
           ISFLAS+E E +KE Y+T+ TPFWGNPNLDLGCWT MP+YDADFGWGRPSYVGP PLVV+
Sbjct: 361 ISFLASKENELVKECYQTEVTPFWGNPNLDLGCWTSMPIYDADFGWGRPSYVGPTPLVVE 420

Query: 423 DGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL 457
           DG SIIM GPDDGSL ++ICLQTK M+EFKKLFYQ+L
Sbjct: 421 DGLSIIMPGPDDGSLILIICLQTKCMNEFKKLFYQHL 457

BLAST of MC01g_new0242 vs. ExPASy TrEMBL
Match: A0A6J1GF56 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111453622 PE=3 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 2.77e-173
Identity = 266/463 (57.45%), Postives = 328/463 (70.84%), Query Frame = 0

Query: 7   SVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNS 66
           S+T+HSS+T+ P+HPTP  TL+ SESDQ     +   IYVY+       +S  +DS++NS
Sbjct: 3   SLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAV--PTSAVVDSLKNS 62

Query: 67  LSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQL 126
           LSQILVPYYPL GRL WI GGRLE+ CC AG QL   EAS+ A  DDYGDF P+DA R+L
Sbjct: 63  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQL--IEASSTATLDDYGDFSPTDALREL 122

Query: 127 VPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGEKT 186
            PK DY  PIE+ P++LVQ+TRFSCGG+VIGI +SH +VDG+S ITFINSWASIAR EKT
Sbjct: 123 APKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKT 182

Query: 187 VESIIPKWPNHERDFLRPVLDPLH---FHHQEYESPPLLIGHSCVEEERNKQTTVALLKL 246
           VESI+   P H+R  L+P   PL    F H EY +PPLL+G S  +EER K++TV LLKL
Sbjct: 183 VESIVQ--PFHDRRILQPQ-KPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKL 242

Query: 247 TKHQHEKLKKIANEGSSML------RPYTRFEAVGGHIWACACKARNSTTTCESDQPTAA 306
           +K Q EKLKK AN  +         RPYTR+E+V GH+W CACKAR++    ++ QPT  
Sbjct: 243 SKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTN---DNSQPTMV 302

Query: 307 LITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYI 366
            +   +R+R+R P P  F GN+TL+ VTP C+F DL+  PLSYAAGKIREGTWK+TDEY 
Sbjct: 303 RVAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYA 362

Query: 367 KSAISFLASRE-IEWIKEWYRTKA---TPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVG 426
           +SA+ FLAS+E I W++  Y TK     PFWGNPNL LG W  +PLY+ADFGWG P YVG
Sbjct: 363 RSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVG 422

Query: 427 PIPLVVDDGWSIIMTGPD-DGSLAVVICLQTKHMDEFKKLFYQ 455
           P  L  D G S IM  PD DG L + + LQ KHMD+FK+LFY+
Sbjct: 423 PATLNAD-GKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 454

BLAST of MC01g_new0242 vs. ExPASy TrEMBL
Match: A0A6J1GCX6 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111453028 PE=3 SV=1)

HSP 1 Score: 497 bits (1279), Expect = 7.66e-171
Identity = 261/462 (56.49%), Postives = 324/462 (70.13%), Query Frame = 0

Query: 7   SVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNS 66
           S+T+HSS+T+ P+HPTP  TL+ SESDQ     +   +YVY+       +S  ++S+RNS
Sbjct: 3   SLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPTV--TTSAVVESLRNS 62

Query: 67  LSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQL 126
           LSQ+LVPYYPLAGRL WI GGRLE+ CC AG QL   EAS+ A  DDYGDF P+DA R+L
Sbjct: 63  LSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQL--IEASSTATLDDYGDFSPTDALREL 122

Query: 127 VPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGEKT 186
            PK DY  PIE+ P+ L+QVTRFSCGG+VIG  +SH +VDG+S ITFINSWASIAR +KT
Sbjct: 123 APKVDYSTPIEELPVFLIQVTRFSCGGIVIGTGLSHILVDGVSAITFINSWASIARKDKT 182

Query: 187 VESIIPKWPNHERDFLRPV--LDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALLKLT 246
           VESI+   P H+R  L+P   L P  F H+EY++PP L+G S  +EER K++   LLKL+
Sbjct: 183 VESIVQ--PFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLKLS 242

Query: 247 KHQHEKLKKIANEG------SSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAAL 306
           K Q EKLKK AN            RPYTR+E++ GH+W CACKAR+   T +  QPT   
Sbjct: 243 KEQVEKLKKRANSNIVNQLDELPSRPYTRYESIAGHMWVCACKARS---TGDKSQPTMVR 302

Query: 307 ITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIK 366
           +   +R+R+  P P  F GN+TL+ VTP C+FGDLI  PLSYAAGKIREGTWK+TDEY +
Sbjct: 303 VAVDVRRRLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKITDEYAR 362

Query: 367 SAISFLASRE-IEWIKEWYRTKA---TPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGP 426
           SA+ FLAS+E I W++  Y TK     PFWGNPNL LG W  +PLY+ADFGWG+P YVGP
Sbjct: 363 SALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGKPCYVGP 422

Query: 427 IPLVVDDGWSIIMTGPD-DGSLAVVICLQTKHMDEFKKLFYQ 455
             L  D G S IM  PD DG L + I LQ KHMD+FK+ FY+
Sbjct: 423 ATLNAD-GKSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYE 454

BLAST of MC01g_new0242 vs. ExPASy TrEMBL
Match: A0A6J1DRJ9 (spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=LOC111023179 PE=3 SV=1)

HSP 1 Score: 494 bits (1273), Expect = 9.42e-170
Identity = 272/474 (57.38%), Postives = 338/474 (71.31%), Query Frame = 0

Query: 7   SVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNS 66
           S+T++SS+T+ PSHPTP   L+LSE+DQ     ++  +Y+YK      A++  + S++NS
Sbjct: 3   SLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAA-ATAAVLGSLKNS 62

Query: 67  LSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQL 126
           LSQILVPYYPLAGRLR I GGRLE+DCC AG  L EA  S+ AK DDYGDF PSDA R+L
Sbjct: 63  LSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEA--SSTAKLDDYGDFAPSDALRRL 122

Query: 127 VPKPDY-RAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGEK 186
            P  DY  +PIE+ P++LVQVTRFSCGG+V+G+  SH +VDG S ITFINSWAS+ARGEK
Sbjct: 123 APNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEK 182

Query: 187 TVESIIPKWPNHERDFLRPV--LDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALLKL 246
           TV+SI P+ P H+R FL+P   + P  FHH EY+ PPLL G+S  EEE  K++  ALLKL
Sbjct: 183 TVDSI-PQ-PFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKL 242

Query: 247 TKHQHEKLKKIAN----------EGSSML------RPYTRFEAVGGHIWACACKARNSTT 306
           TK Q EKLKK AN          +  +ML      RPY+R+E++ G++W CACKARN+  
Sbjct: 243 TKEQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNA- 302

Query: 307 TCESDQPTAALITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIRE 366
             ES QPT   I   I +R+R P PE F GN+ L+T+TP CKFGDL+  PLSYA GKIRE
Sbjct: 303 --ESRQPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIRE 362

Query: 367 GTWKLTDEYIKSAISFLASRE-IEWIKEWY----RTKATPFWGNPNLDLGCWTLMPLYDA 426
           GTWK+TDEY KSA+ FLA +E I W +  +    R K + FWGNPNL LG W  MPLYDA
Sbjct: 363 GTWKMTDEYAKSAMDFLARKEDITWARTLHHSIVRVKGS-FWGNPNLALGSWMSMPLYDA 422

Query: 427 DFGWGRPSYVGPIPLVVDDGWSIIMTGP-DDGSLAVVICLQTKHMDEFKKLFYQ 455
           DFGWGRPSYVGP  +V  DG + IM+GP +DGS+ + I LQTKHMD+FKK FY+
Sbjct: 423 DFGWGRPSYVGPA-MVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYE 466

BLAST of MC01g_new0242 vs. TAIR 10
Match: AT2G19070.1 (spermidine hydroxycinnamoyl transferase )

HSP 1 Score: 313.2 bits (801), Expect = 3.6e-85
Identity = 177/457 (38.73%), Postives = 262/457 (57.33%), Query Frame = 0

Query: 8   VTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNSL 67
           +T   S TI+P+ PT      L+E DQ     +I  +Y Y K  +    ++ ++ ++ SL
Sbjct: 4   ITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNV-VEILKTSL 63

Query: 68  SQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQLV 127
           S++LV +YP+AGRLRW+  GR E++C   GV+  EAE  ++ K  D+ DF P+     L+
Sbjct: 64  SRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAE--SEGKLSDFKDFSPTPEFENLM 123

Query: 128 PKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARGE--K 187
           P+ +Y+ PIE  PL L QVT+F CGG+ + + VSH +VDG S +  I+ W  +ARGE  +
Sbjct: 124 PQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGEPLE 183

Query: 188 TVESIIPK--WPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALLKL 247
           TV  +  K  W       L P + P  F H+E++ PP LIG +   EER K+T V +L L
Sbjct: 184 TVPFLDRKILWAGEP---LPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLPL 243

Query: 248 TKHQHEKLKKIAN--EGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALITA 307
           +  Q +KL+  AN  + S   + +TR+E V GH+W CACKAR  +     +QPTA  I  
Sbjct: 244 STSQLQKLRSKANGSKHSDPAKGFTRYETVTGHVWRCACKARGHS----PEQPTALGICI 303

Query: 308 SIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSAI 367
             R R+ PP P G+ GNAT L V      G+LI N L +AA  I +    +T+EY+   I
Sbjct: 304 DTRSRMEPPLPRGYFGNAT-LDVVAASTSGELISNELGFAASLISKAIKNVTNEYVMIGI 363

Query: 368 SFLAS-REIEWIKEWYRTKAT--PFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLV 427
            +L + ++++  ++ +   +T  PF+GNPNL +  W  +P+Y  DFGWG+  Y GP    
Sbjct: 364 EYLKNQKDLKKFQDLHALGSTEGPFYGNPNLGVVSWLTLPMYGLDFGWGKEFYTGPGTHD 423

Query: 428 VDDGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQ 456
            D    I+    +DGS+ +  CLQ  HM+ FKK FY+
Sbjct: 424 FDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYE 449

BLAST of MC01g_new0242 vs. TAIR 10
Match: AT5G48930.1 (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase )

HSP 1 Score: 262.3 bits (669), Expect = 7.2e-70
Identity = 162/455 (35.60%), Postives = 247/455 (54.29%), Query Frame = 0

Query: 8   VTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDS--MRN 67
           + +  S+ + P+  TP   L  S  D  +   +   +Y Y+       +S   D   M+ 
Sbjct: 3   INIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYRPT----GASNFFDPQVMKE 62

Query: 68  SLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQ 127
           +LS+ LVP+YP+AGRL+  D GR+EIDC GAGV      A   +  DD+GDF P+   RQ
Sbjct: 63  ALSKALVPFYPMAGRLKRDDDGRIEIDCNGAGVLF--VVADTPSVIDDFGDFAPTLNLRQ 122

Query: 128 LVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARG-E 187
           L+P+ D+ A I   PL+++QVT F CGG  +G+ + H   DG S + FIN+W+ +ARG +
Sbjct: 123 LIPEVDHSAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMARGLD 182

Query: 188 KTVESIIPKWPNHERDFLRPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQTTVALLKLT 247
            T+   I +     RD  +P      FHH EY+  P +       +   + TTV++ KLT
Sbjct: 183 LTIPPFIDRTLLRARDPPQPA-----FHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLT 242

Query: 248 KHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALITASIR 307
           + Q   LK  + E  + +  Y+ +E + GH+W    KAR       +DQ T   I    R
Sbjct: 243 RDQLVALKAKSKEDGNTV-SYSSYEMLAGHVWRSVGKARG----LPNDQETKLYIATDGR 302

Query: 308 QRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSAISFL 367
            R+RP  P G+ GN  + T TP+   GDL+  P  YAAG+I +   ++ D Y++SA+ +L
Sbjct: 303 SRLRPQLPPGYFGN-VIFTATPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNYLRSALDYL 362

Query: 368 ASR-EIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGP--IPLVVDD 427
             + ++  +     T     +  PNL +  W  +P+YDADFGWGRP ++GP  IP    +
Sbjct: 363 EMQPDLSALVRGAHT-----YKCPNLGITSWVRLPIYDADFGWGRPIFMGPGGIPY---E 422

Query: 428 GWSIIMTGP-DDGSLAVVICLQTKHMDEFKKLFYQ 456
           G S ++  P +DGSL+V I LQ++HM  F+K  ++
Sbjct: 423 GLSFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of MC01g_new0242 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 185.7 bits (470), Expect = 8.6e-47
Identity = 131/413 (31.72%), Postives = 195/413 (47.22%), Query Frame = 0

Query: 60  IDSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDF-- 119
           +  ++ +LSQ+LV YYPLAGRL     G+L +DC   GV   EAE  A+ K D+ GD   
Sbjct: 82  VQVIKKALSQVLVHYYPLAGRLTISPEGKLTVDCTEEGVVFVEAE--ANCKMDEIGDITK 141

Query: 120 -EPSDATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINS 179
            +P    + +    D +  +E  P V  QVT+F CGG V+G+ ++H + DGI  + F+NS
Sbjct: 142 PDPETLGKLVYDVVDAKNILEIPP-VTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNS 201

Query: 180 WASIARGEKTVESIIPKWPNHERDFLR----PVLDPLHFHHQE----------YESPPLL 239
           W  +ARG           P  +R  L     P ++ LH   +E          Y   P L
Sbjct: 202 WGQVARGLPLTTP-----PFSDRTILNARNPPKIENLHQEFEEIEDKSNINSLYTKEPTL 261

Query: 240 IGHSCVEEERNKQTTVALLKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKA 299
               C + E+ K+              KL+   N  S +    T FEA+   +W    +A
Sbjct: 262 YRSFCFDPEKIKKL-------------KLQATENSESLLGNSCTSFEALSAFVW----RA 321

Query: 300 RNSTTTCESDQPTAALITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAA 359
           R  +    SDQ T  L     R +  P  P+G+ GN  +LT   IC+ G+LI+ PLS+A 
Sbjct: 322 RTKSLKMLSDQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLT-NSICEAGELIEKPLSFAV 381

Query: 360 GKIREGTWKLTDEYIKSAISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDA 419
           G +RE    +TD Y++SAI +    E+        T+A P   +  L +  W+ +  +  
Sbjct: 382 GLVREAIKMVTDGYMRSAIDYF---EV--------TRARPSLSS-TLLITTWSRLGFHTT 441

Query: 420 DFGWGRPSYVGPIPLVVDDGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQ 456
           DFGWG P   GP+ L   +    +  G    S+ V++ L    MD F++ F Q
Sbjct: 442 DFGWGEPILSGPVALPEKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 456

BLAST of MC01g_new0242 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 185.7 bits (470), Expect = 8.6e-47
Identity = 131/413 (31.72%), Postives = 195/413 (47.22%), Query Frame = 0

Query: 60  IDSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDF-- 119
           +  ++ +LSQ+LV YYPLAGRL     G+L +DC   GV   EAE  A+ K D+ GD   
Sbjct: 66  VQVIKKALSQVLVHYYPLAGRLTISPEGKLTVDCTEEGVVFVEAE--ANCKMDEIGDITK 125

Query: 120 -EPSDATRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINS 179
            +P    + +    D +  +E  P V  QVT+F CGG V+G+ ++H + DGI  + F+NS
Sbjct: 126 PDPETLGKLVYDVVDAKNILEIPP-VTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNS 185

Query: 180 WASIARGEKTVESIIPKWPNHERDFLR----PVLDPLHFHHQE----------YESPPLL 239
           W  +ARG           P  +R  L     P ++ LH   +E          Y   P L
Sbjct: 186 WGQVARGLPLTTP-----PFSDRTILNARNPPKIENLHQEFEEIEDKSNINSLYTKEPTL 245

Query: 240 IGHSCVEEERNKQTTVALLKLTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKA 299
               C + E+ K+              KL+   N  S +    T FEA+   +W    +A
Sbjct: 246 YRSFCFDPEKIKKL-------------KLQATENSESLLGNSCTSFEALSAFVW----RA 305

Query: 300 RNSTTTCESDQPTAALITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAA 359
           R  +    SDQ T  L     R +  P  P+G+ GN  +LT   IC+ G+LI+ PLS+A 
Sbjct: 306 RTKSLKMLSDQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLT-NSICEAGELIEKPLSFAV 365

Query: 360 GKIREGTWKLTDEYIKSAISFLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDA 419
           G +RE    +TD Y++SAI +    E+        T+A P   +  L +  W+ +  +  
Sbjct: 366 GLVREAIKMVTDGYMRSAIDYF---EV--------TRARPSLSS-TLLITTWSRLGFHTT 425

Query: 420 DFGWGRPSYVGPIPLVVDDGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQ 456
           DFGWG P   GP+ L   +    +  G    S+ V++ L    MD F++ F Q
Sbjct: 426 DFGWGEPILSGPVALPEKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 440

BLAST of MC01g_new0242 vs. TAIR 10
Match: AT3G48720.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 173.7 bits (439), Expect = 3.4e-43
Identity = 138/450 (30.67%), Postives = 221/450 (49.11%), Query Frame = 0

Query: 8   VTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDIDASSLTIDSMRNSL 67
           VT  +   I P   TP+    LS  DQ  +   +  +Y YK     +  S  +  ++ SL
Sbjct: 9   VTRKNPELIPPVSETPNGHYYLSNLDQ-NIAIIVKTLYYYKSESRTNQESYNV--IKKSL 68

Query: 68  SQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEPSDATRQLV 127
           S++LV YYP+AGRL     G++ ++C G GV + EAEA+      D      S+   + +
Sbjct: 69  SEVLVHYYPVAGRLTISPEGKIAVNCTGEGVVVVEAEANCGI---DTIKEAISENRMETL 128

Query: 128 PKPDYRAP----IEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSWASIARG 187
            K  Y  P    I + P V+VQVT F CGG V+G+ +SH + DG++   F+NSW  +A+G
Sbjct: 129 EKLVYDVPGARNILEIPPVVVQVTNFKCGGFVLGLGMSHNMFDGVAAAEFLNSWCEMAKG 188

Query: 188 EKTVESIIPKWPNHERDFLRPVLDP-LHFHHQEYESPPLLIGHSCVEEERNKQTTVALLK 247
              +   +P  P  +R  LR    P + F H E++    +     + +E        L +
Sbjct: 189 ---LPLSVP--PFLDRTILRSRNPPKIEFPHNEFDEIEDISDTGKIYDEEKLIYKSFLFE 248

Query: 248 LTKHQHEKLKKIANEGSSMLRPYTRFEAVGGHIWACACKARNSTTTCESDQPTAALITAS 307
             K   EKLK +A E ++  +  T F+A+ G +W   C+A       + DQ    L  A 
Sbjct: 249 PEK--LEKLKIMAIEENNNNKVST-FQALTGFLWKSRCEA----LRFKPDQRVKLLFAAD 308

Query: 308 IRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGTWKLTDEYIKSAIS 367
            R R  P  P+G+CGN  +LT   +   G+L+ NPLS++ G ++     +TD +++SA+ 
Sbjct: 309 GRSRFIPRLPQGYCGNGIVLTGL-VTSSGELVGNPLSHSVGLVKRLVELVTDGFMRSAMD 368

Query: 368 FLASREIEWIKEWYRTKATPFWGNPNLDLGCWTLMPLYDADFGWGRPSYVGPIPLVVDDG 427
           +          E  RT+ +    N  L +  W+ + L+  DFGWG P + GP+ L   + 
Sbjct: 369 YF---------EVNRTRPSM---NATLLITSWSKLTLHKLDFGWGEPVFSGPVGLPGREV 427

Query: 428 WSIIMTGPDDGSLAVVICLQTKHMDEFKKL 453
              + +G D  S+ V + L T  M+ F++L
Sbjct: 429 ILFLPSGDDMKSINVFLGLPTSAMEVFEEL 427