MC01g_new0236 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g_new0236 vs. NCBI nr
Match: KQT74506.1 (hypothetical protein ASG45_08000 [Microbacterium sp. Leaf436])

HSP 1 Score: 90.9 bits (224), Expect = 3.84e-15
Identity = 100/395 (25.32%), Postives = 187/395 (47.34%), Query Frame = 0

Query: 460 LDALQKKARIASLE-----EEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPR 519
           L++++    + SLE     E V S  +  S + VE    L++++    + SLE    S  
Sbjct: 3   LESVESLESVESLESVMSLESVESLESVMSLESVESVESLESVESLESVMSLE----SVE 62

Query: 520 TFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSL 579
           +F+S + +E    L++ +    + SLE E     +F+S + +E    L++ +    + SL
Sbjct: 63  SFESVESLESVMSLESFESLESVESLEVE-----SFESVESLESVMSLESFESLESVESL 122

Query: 580 EEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQ 639
           E    S  + +S + +E    L++ + +  + SLE  V S  +F+S + VE    +++L+
Sbjct: 123 E----SVESLESVESLESVMSLESFESQESVESLES-VMSLESFESQESVESLESVESLE 182

Query: 640 KKARIPSLE--------EEVTSLRTFDSAQKVELCYLLDALQKKARIASLE-----EEVT 699
               + S E        E V SL + +S + V  C  L++ +    + S E     E V 
Sbjct: 183 SVMSLESFESQESVESLESVESLESVESLESVASCESLESFESLESVESQESVESQESVE 242

Query: 700 SPRTFDSAQKVELCYLLDALQKKARIASLE--EEVTSPRTFDSAQNVELCYLLDALQKRA 759
           S  + +S + VE    +++L+      S+E  E V S  +F+S ++VE    L++++   
Sbjct: 243 SLESVESQESVESQESVESLESVESQESVESLESVMSLESFESQESVES---LESVESSE 302

Query: 760 IIPSLEEASSLRTFDSAQKVELCYLLDALQKRTTILSLVGEKMAIIPSLEEKVMSPRTFE 819
            + SLE   SL + +S + V  C   ++L+ + ++ S   E +  + S +E V S  + E
Sbjct: 303 SVMSLESVMSLESVESLESVASCESFESLESQESVESF--ESLESVES-QESVESFESLE 362

Query: 820 SAQKEELCYLLDALQKMAIIPSSLEKVMSLMSYGS 834
           S + +E    L+++        SLE V SL S  S
Sbjct: 363 SVESQESVESLESVMSWESF-ESLESVESLESLES 376

BLAST of MC01g_new0236 vs. NCBI nr
Match: XP_013182958.1 (PREDICTED: microtubule-associated protein futsch [Amyelois transitella])

HSP 1 Score: 85.1 bits (209), Expect = 1.64e-12
Identity = 185/796 (23.24%), Postives = 333/796 (41.83%), Query Frame = 0

Query: 27   VPSSLEEVMSPRTFDSVQKVELCYLLDGLQKRVIIPSLEEEVTSLRTFDSAQKVELCYLL 86
            V SS +EV  P    S +K          +K +I+P L++         SA+K     +L
Sbjct: 4781 VLSSEKEVAVPVVDKSKEK--------SREKEIIVPVLDKSKEP-----SAEKEVAVPVL 4840

Query: 87   DALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKRAIIPSLEEEVTSLRTFDSAQ 146
            D    K + PS E+EVT P    S +      +   +  ++  PS EE+VT      S +
Sbjct: 4841 D----KPKEPSPEKEVTLPVLDKSKEPTPEKEIHVPVSDKSKEPSPEEKVTLPVLDKSKE 4900

Query: 147  KVELCYFLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEE---E 206
                      +  K++ PS E+EV  P +  S +       +  L K   ++  +E   +
Sbjct: 4901 PTPEKEIHVPVSDKSKEPSPEKEVPVPVSDKSEESSPETVSVPVLDKSKELSPEKEVPMQ 4960

Query: 207  VTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSP----RTFDSAQKVELCYLLDAL 266
            V       S +K     +LD    K++ PS E+EV  P        S +K +   +LD  
Sbjct: 4961 VLDKSKEPSPKKEVSVPVLD----KSKEPSTEKEVAEPVLDKSKEPSPEKEDTVPVLD-- 5020

Query: 267  QKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVE 326
              K++ P+ E+EVT P    S +      +   +  K++ PS EE+VT P    S +   
Sbjct: 5021 --KSKEPTPEKEVTLPVLDKSKEPTPEKEIHVPVSDKSKEPSPEEKVTLPVLDKSKEPTP 5080

Query: 327  LCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRT 386
               +   +  K+  +S E +V+ P    S +      +   +  +++  S E+EV  P++
Sbjct: 5081 EKEIHVPVSDKSEESSPETDVSVPVLDKSKELSPEKEVAVPVLDESKEPSPEKEVAVPKS 5140

Query: 387  FDSAQKVELCY-LLD-----ALQKKARIASL--------EEEVTSPRTFDSAQKVELCYL 446
             + + + E+   +LD     + +K+  +  L        E+EV  P    S +      +
Sbjct: 5141 KEPSPEKEVAVPVLDKSKDPSPEKEVAVLVLDKSKEPGPEKEVAVPVLDKSKEPSPEKEV 5200

Query: 447  LDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSA 506
               +  K++ +S E+EV  P    S +      +   ++ K++  S E+EV  P    S 
Sbjct: 5201 PVPVLDKSKESSSEKEVAVPVLDKSKEPSPEKEVAVPVEDKSKEPSPEKEVAVPVEDKSK 5260

Query: 507  QKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVT 566
            +      +   ++ K++ PS E+EV  P    S +      +   +  K++ PS E+EV 
Sbjct: 5261 EPSSEKEVAVPVEDKSKEPSPEKEVAVPVEDKSKEPSPEKEVAVPVLDKSKEPSSEKEVA 5320

Query: 567  SPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARI 626
             P    S +      +  ++  K++ PS E++VT P    S +   +  +   +  K++ 
Sbjct: 5321 VPVEDKSKEPSPEKEVTVSVLDKSKEPSPEKKVTVPVLDKSKEPSPVKEVTVPVLDKSKE 5380

Query: 627  PSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSLRTFDSAQKVELCYLLD 686
            PSLE+EVT P    S +      +   +  K + PSLE+EVT                L 
Sbjct: 5381 PSLEKEVTVPVLHKSKEPSPEKEVTVPVLDKFKEPSLEKEVT----------------LP 5440

Query: 687  ALQKKARIASLEEEVTSPRTFDSAQKVELCYLL----DALQKKARIASLEEEVTSPRTFD 746
             L K     ++ E+ T     +S+ K++  Y+     D+      + S    ++SP    
Sbjct: 5441 VLDKSKE--TMPEKDTGISIQESSSKLDESYIQGIADDSDIPSPELVSDSSNISSPEKIR 5500

Query: 747  SAQNVELCYLLDALQKRAIIP--SLEEASSLRTFDSAQKV--ELCYLLDALQKRTTILSL 793
            S  N+E        +   I+P  +L ++      DS+ KV  ++   LD L+     L++
Sbjct: 5501 STLNIE--------KFEKIVPDVALNKSKKQSEADSSVKVSDDVFDTLDELKG----LAV 5521

BLAST of MC01g_new0236 vs. NCBI nr
Match: XP_001744383.1 (uncharacterized protein MONBRDRAFT_6845 [Monosiga brevicollis MX1] >EDQ91086.1 predicted protein [Monosiga brevicollis MX1])

HSP 1 Score: 73.9 bits (180), Expect = 3.12e-09
Identity = 186/764 (24.35%), Postives = 338/764 (44.24%), Query Frame = 0

Query: 63  SLEEEVTSLRTFDSAQKVELCY---LLDALQKKARIPSLEEEVTSPRTFDSAQKVELC-- 122
           +LEEE+ +LR    +   EL      ++ LQ++        +  + R   +  K++    
Sbjct: 260 ALEEELITLRAMQQSADPELVENKSSVNELQQQLEAAQESHQAAAERASAAQVKLDTMTQ 319

Query: 123 -YLLDALQKRAIIPSLEEEVTSLRT-FDSAQKVELCYFLDALQKKARIPSLEEEVTSPRT 182
            Y     ++RA +  LE ++TSLR   DSA+       LDA Q +A++ +L EE+ S R 
Sbjct: 320 QYAARDQEQRAKVEELELDLTSLRAELDSARAASEASQLDAKQLEAQVATLTEELDSARA 379

Query: 183 FDSAQKVELCYLLDALQKKARIASLEEEVT--SPRTFDSAQKVELCY----------LLD 242
              A +      LDA Q +A++A+L EE+   S R   +  +VE             L +
Sbjct: 380 ASEASQ------LDAKQLEAQVATLTEELDHLSDREQQAQSQVEAAQQAAETAQALLLKE 439

Query: 243 ALQKKARIPSLEEEVTSPRTFDSAQKVELCYL-LDALQKKARIPSLEEEVTSPRTFDSAQ 302
            L ++A     EE     +T  +A + EL ++ L   Q++  I + + ++ +   ++S Q
Sbjct: 440 ELSRQASEARAEEA----QTHVAALEEELQHVGLKVKQQETEIHACQSKIEA---YESEQ 499

Query: 303 KVELCY------LLDALQKKARIPSLEEEVTSPRT-FDSAQKVELCYLLDALQKKARIAS 362
           + +         LLDA   +AR   L+E+  + R+ ++  +     +  +    +A++ +
Sbjct: 500 QQQALVEAKDEELLDAT--RARFNELQEQHEALRSNYEKLKSKAQGFAAELKTARAKLRA 559

Query: 363 LEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDAL 422
            +EE T  RT   A +VE    +DA + +A +AS+E           A++ +L   L+ L
Sbjct: 560 HDEEETQ-RT---AAQVEAQAHVDA-EAQANLASME-----------AERAQLATKLNTL 619

Query: 423 QKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVE 482
           Q  AR+A +E+E+ +     + ++ EL   L+  Q   ++A+ +    +      A + E
Sbjct: 620 Q--ARLAGVEDELDA----SNEEREELA--LELSQSNEQLAAAQVTAKAAEKNLHALRAE 679

Query: 483 LCYLL----DALQKKARIASLEEEVTSPRTFDSAQKVE-LCYLLDAL-QKKARIPSLEEE 542
           L  L     +A+Q   + A  E +V      +S Q+++ L   L+A+ Q++  +    EE
Sbjct: 680 LTTLKRERDEAIQALKQHAQHETDVAG----ESRQRLDALSAELEAVEQERDELLQELEE 739

Query: 543 VTSPR------TFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLD 602
           +T+ R      T   AQ++E       L+ + ++ ++E+++   R  +S+ + E   + +
Sbjct: 740 LTAERATSQESTTKHAQEMERTRKA-LLEAQTQLQAVEQQLEEQRQQNSSLQDEHATVQN 799

Query: 603 ALQKKARIPSLEE-EVTSPRTFDSAQKVELCYL--LDALQKKARIPS--LEEEVTSPRTF 662
            L   A I  L++ E  S +    A +     +  L+A  K AR      EE+++     
Sbjct: 800 ELT--ATISRLQDQEAASAQAIADANRAAATRVADLEADLKTARHERDVFEEQLSQASNA 859

Query: 663 DSAQKVELCYLLDALQKKARIPSLEEEVTSLRTFDSAQKVELCYLLDALQKKARIASLEE 722
            +   V    L    +  ARI  LEEE+T  R   S  + EL  + D L++  R     +
Sbjct: 860 AATVAVAEPPLQTTKEADARIALLEEELTGTRQELSDVREELRQVQDELEQLLR-----D 919

Query: 723 EVTSPRTFDSAQKV--ELCYLLDALQKKAR---------IASLEEEVTSPRTFDSAQNVE 771
           +  S  T    ++V  EL + L  L + A          + +  +   S    D+   V 
Sbjct: 920 QEASDATIQEYEEVIQELEHKLARLDQPAEDTGRNQSEPVVNSNDIPESSVPADTTDLVS 964

BLAST of MC01g_new0236 vs. NCBI nr
Match: WP_207573136.1 (YSIRK-type signal peptide-containing protein [Staphylococcus nepalensis] >MBO1217014.1 YSIRK-type signal peptide-containing protein [Staphylococcus nepalensis])

HSP 1 Score: 73.2 bits (178), Expect = 6.93e-09
Identity = 189/898 (21.05%), Postives = 367/898 (40.87%), Query Frame = 0

Query: 2    SPRTFDFVQIEELGYLLDAL-QHKTIVPSSLEEVMSPRTFDSVQKVELCYLL-------- 61
            S    D  Q+ E    ++A+ + K  V + L E+ S    +  +K EL  L+        
Sbjct: 1224 SNGVLDTEQLSEAEQAIEAVGEAKKGVDNKLAEITSDNLVNPREKAELEQLIEALETAKA 1283

Query: 62   ----------------DGLQKR------VIIPSLEEEVTSLRTFDSAQKVELCYLLDALQ 121
                            DGLQ R      V +P + ++  +    D+ Q  +    + A++
Sbjct: 1284 EASTKLNNVPTGTTGKDGLQTRLDQIGSVTVPEVNDQDAN-GVLDTEQLSKAEEAIAAVE 1343

Query: 122  K-KARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKRAIIPSLEEEVTSLRTFDSAQKVE 181
            + K  + +   EVTS    + ++K EL  L++AL+   +   +  ++ ++ T  +A K E
Sbjct: 1344 EVKKTVDNKLSEVTSDGLVNPSEKAELEQLIEALETAKV--DVSTKLNNVPT-GTAGKAE 1403

Query: 182  LCYFLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQK-KARIASLEEEVTSPR 241
            L   LD +     +P + ++  S    D+ Q  E    ++A+++ K  + +   E+T+  
Sbjct: 1404 LQTRLDQIGT-VTLPEVNDQ-DSNGVLDTDQLSEAEEAIEAVEEAKKAVDNKLSEITADN 1463

Query: 242  TFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSL 301
              + ++K EL  L++AL+  A++ +  +    P    +  K EL   LD +      P +
Sbjct: 1464 LINPSEKAELEQLIEALET-AKVEASTKLNNVPN--GTPGKAELQTRLDQIDS-VTAPEV 1523

Query: 302  EEEVTSPRTFDSAQKVELCYLLDAL-QKKARIPSLEEEVTSPRTFDSAQKVELCYLLDAL 361
             ++       D+ Q  E    ++A+ Q K+ + +   E+TS    + ++K EL  L++AL
Sbjct: 1524 NDQ-DGNGVLDTKQLSEAEQAIEAVEQTKSAVANKLTEITSDGLVNPSEKAELDQLIEAL 1583

Query: 362  QKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRT-------- 421
            +  A++ +  +  + P    +  K EL   LD +          + VT+P          
Sbjct: 1584 ET-AKVEASAKLNSVPN--GTPGKDELQTRLDQI----------DSVTAPEVNDQDGNGV 1643

Query: 422  FDSAQKVELCYLLDAL-QKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASL 481
             D+ Q  E    ++A+ Q K+ +A+   E+TS    + ++K+EL  L++AL+     AS 
Sbjct: 1644 LDTKQLSEAEQAIEAVEQTKSAVANKLTEITSDGLVNPSEKLELDQLIEALETTKAEASA 1703

Query: 482  E---------------------EEVTSPR--------TFDSAQKVELCYLLDALQK-KAR 541
            +                     + VTSP           D+ Q  E    + A+++ K  
Sbjct: 1704 KLNSVPDGTTGKDGLQTRLDQIDTVTSPEINDQDSNGVLDTEQLSEAEEAIKAVEEAKKA 1763

Query: 542  IASLEEEVTSPRTFDSAQKVELCYLLDALQK-----KARIPSLEEEVTSPRTFDSAQKVE 601
            + +   E+T+    + ++K EL  L++AL+       A++ S+ +  T         K E
Sbjct: 1764 VDNKLSEITADNLINPSEKAELDQLIEALETAKAEASAKLNSVPDGTTG--------KAE 1823

Query: 602  LCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQK-KARIPSLEEEVTSPR 661
            L   LD +     +P + ++       D+ Q  E    ++A++K K  + +   E+T+  
Sbjct: 1824 LQTRLDQIGT-VTLPKVNDQ-DGNGVLDTEQLSEAAQAIEAMEKAKKAVDNKLSEITADN 1883

Query: 662  TFDSAQKVELCYLLDALQK-KARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPS 721
              +  +K EL  L++ L+  K         V +  T     +  L  +          P 
Sbjct: 1884 LINPREKAELEELIEVLETTKVNASEKLNNVPNGTTGKDGLQTRLNQI-----GSVTTPE 1943

Query: 722  LEEEVTSLRTFDSAQKVELCYLLDALQKKARIASLE-EEVTSPRTFDSAQKVELCYLLDA 781
            + +  ++    D+ Q  E    + A+++  + A+ +  E+TS    + ++K EL  L+ A
Sbjct: 1944 VNDRDSN-GVKDTEQLSEASQAITAVEEAKKAAANKLSEITSDNLVNPSEKAELDQLIGA 2003

Query: 782  LQKKARIASLE---------------------EEVTSPRTFDSAQN----------VELC 787
            L+     AS +                       VT+P   D   N           E  
Sbjct: 2004 LETAKAEASTKLNNVPDGTTGKDELQTRLGEIGSVTTPEVNDQDSNGVLDTEQLSEAEAA 2063

BLAST of MC01g_new0236 vs. NCBI nr
Match: WP_176388568.1 (phage tail protein [Janthinobacterium sp. BJB401] >NVI81928.1 phage tail protein [Janthinobacterium sp. BJB401])

HSP 1 Score: 69.3 bits (168), Expect = 6.29e-08
Identity = 53/253 (20.95%), Postives = 116/253 (45.85%), Query Frame = 0

Query: 268 LEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDAL 327
           ++++  + R +  AQ  +    +D     A++  ++++ TS RT+   Q  +     D  
Sbjct: 151 VDDQDITVRAYSDAQLAKHLAAVDPHPLLAKVTYVDQQDTSARTYGDQQLAKHQAAADPH 210

Query: 328 QKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVE 387
              A++  ++++ TS RT+   Q  +     D     A++  ++++ TS RT+   Q  +
Sbjct: 211 PLLAKVTYVDQQDTSARTYSDQQLAKHQAAADPHPLLAKVTYVDQQDTSARTYGDQQLAK 270

Query: 388 LCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRT 447
                DA    A++  ++++  S RT+   Q  +     D     A++  ++++  S R 
Sbjct: 271 HQAATDAHPLLAKVTYVDQQDASARTYGDQQLAKHQAAADPHPLLAKVTYVDQQDASARA 330

Query: 448 FDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLE 507
           +   Q  +     D     A++A ++++ TS RT+   Q  +     DA    A++  ++
Sbjct: 331 YGDQQLAKHQAAADPHPLLAKVAYVDQQDTSARTYGDQQLAKHQAATDAHPLLAKVTYVD 390

Query: 508 EEVTSPRTFDSAQ 520
           ++VTS RT+   Q
Sbjct: 391 QQVTSARTYGDQQ 403

BLAST of MC01g_new0236 vs. ExPASy TrEMBL
Match: A0A0Q6E7P6 (Uncharacterized protein OS=Microbacterium sp. Leaf436 OX=1736377 GN=ASG45_08000 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.86e-15
Identity = 100/395 (25.32%), Postives = 187/395 (47.34%), Query Frame = 0

Query: 460 LDALQKKARIASLE-----EEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPR 519
           L++++    + SLE     E V S  +  S + VE    L++++    + SLE    S  
Sbjct: 3   LESVESLESVESLESVMSLESVESLESVMSLESVESVESLESVESLESVMSLE----SVE 62

Query: 520 TFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSL 579
           +F+S + +E    L++ +    + SLE E     +F+S + +E    L++ +    + SL
Sbjct: 63  SFESVESLESVMSLESFESLESVESLEVE-----SFESVESLESVMSLESFESLESVESL 122

Query: 580 EEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQ 639
           E    S  + +S + +E    L++ + +  + SLE  V S  +F+S + VE    +++L+
Sbjct: 123 E----SVESLESVESLESVMSLESFESQESVESLES-VMSLESFESQESVESLESVESLE 182

Query: 640 KKARIPSLE--------EEVTSLRTFDSAQKVELCYLLDALQKKARIASLE-----EEVT 699
               + S E        E V SL + +S + V  C  L++ +    + S E     E V 
Sbjct: 183 SVMSLESFESQESVESLESVESLESVESLESVASCESLESFESLESVESQESVESQESVE 242

Query: 700 SPRTFDSAQKVELCYLLDALQKKARIASLE--EEVTSPRTFDSAQNVELCYLLDALQKRA 759
           S  + +S + VE    +++L+      S+E  E V S  +F+S ++VE    L++++   
Sbjct: 243 SLESVESQESVESQESVESLESVESQESVESLESVMSLESFESQESVES---LESVESSE 302

Query: 760 IIPSLEEASSLRTFDSAQKVELCYLLDALQKRTTILSLVGEKMAIIPSLEEKVMSPRTFE 819
            + SLE   SL + +S + V  C   ++L+ + ++ S   E +  + S +E V S  + E
Sbjct: 303 SVMSLESVMSLESVESLESVASCESFESLESQESVESF--ESLESVES-QESVESFESLE 362

Query: 820 SAQKEELCYLLDALQKMAIIPSSLEKVMSLMSYGS 834
           S + +E    L+++        SLE V SL S  S
Sbjct: 363 SVESQESVESLESVMSWESF-ESLESVESLESLES 376

BLAST of MC01g_new0236 vs. ExPASy TrEMBL
Match: A9UUF0 (Predicted protein OS=Monosiga brevicollis OX=81824 GN=6845 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.51e-09
Identity = 186/764 (24.35%), Postives = 338/764 (44.24%), Query Frame = 0

Query: 63  SLEEEVTSLRTFDSAQKVELCY---LLDALQKKARIPSLEEEVTSPRTFDSAQKVELC-- 122
           +LEEE+ +LR    +   EL      ++ LQ++        +  + R   +  K++    
Sbjct: 260 ALEEELITLRAMQQSADPELVENKSSVNELQQQLEAAQESHQAAAERASAAQVKLDTMTQ 319

Query: 123 -YLLDALQKRAIIPSLEEEVTSLRT-FDSAQKVELCYFLDALQKKARIPSLEEEVTSPRT 182
            Y     ++RA +  LE ++TSLR   DSA+       LDA Q +A++ +L EE+ S R 
Sbjct: 320 QYAARDQEQRAKVEELELDLTSLRAELDSARAASEASQLDAKQLEAQVATLTEELDSARA 379

Query: 183 FDSAQKVELCYLLDALQKKARIASLEEEVT--SPRTFDSAQKVELCY----------LLD 242
              A +      LDA Q +A++A+L EE+   S R   +  +VE             L +
Sbjct: 380 ASEASQ------LDAKQLEAQVATLTEELDHLSDREQQAQSQVEAAQQAAETAQALLLKE 439

Query: 243 ALQKKARIPSLEEEVTSPRTFDSAQKVELCYL-LDALQKKARIPSLEEEVTSPRTFDSAQ 302
            L ++A     EE     +T  +A + EL ++ L   Q++  I + + ++ +   ++S Q
Sbjct: 440 ELSRQASEARAEEA----QTHVAALEEELQHVGLKVKQQETEIHACQSKIEA---YESEQ 499

Query: 303 KVELCY------LLDALQKKARIPSLEEEVTSPRT-FDSAQKVELCYLLDALQKKARIAS 362
           + +         LLDA   +AR   L+E+  + R+ ++  +     +  +    +A++ +
Sbjct: 500 QQQALVEAKDEELLDAT--RARFNELQEQHEALRSNYEKLKSKAQGFAAELKTARAKLRA 559

Query: 363 LEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDAL 422
            +EE T  RT   A +VE    +DA + +A +AS+E           A++ +L   L+ L
Sbjct: 560 HDEEETQ-RT---AAQVEAQAHVDA-EAQANLASME-----------AERAQLATKLNTL 619

Query: 423 QKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVE 482
           Q  AR+A +E+E+ +     + ++ EL   L+  Q   ++A+ +    +      A + E
Sbjct: 620 Q--ARLAGVEDELDA----SNEEREELA--LELSQSNEQLAAAQVTAKAAEKNLHALRAE 679

Query: 483 LCYLL----DALQKKARIASLEEEVTSPRTFDSAQKVE-LCYLLDAL-QKKARIPSLEEE 542
           L  L     +A+Q   + A  E +V      +S Q+++ L   L+A+ Q++  +    EE
Sbjct: 680 LTTLKRERDEAIQALKQHAQHETDVAG----ESRQRLDALSAELEAVEQERDELLQELEE 739

Query: 543 VTSPR------TFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLD 602
           +T+ R      T   AQ++E       L+ + ++ ++E+++   R  +S+ + E   + +
Sbjct: 740 LTAERATSQESTTKHAQEMERTRKA-LLEAQTQLQAVEQQLEEQRQQNSSLQDEHATVQN 799

Query: 603 ALQKKARIPSLEE-EVTSPRTFDSAQKVELCYL--LDALQKKARIPS--LEEEVTSPRTF 662
            L   A I  L++ E  S +    A +     +  L+A  K AR      EE+++     
Sbjct: 800 ELT--ATISRLQDQEAASAQAIADANRAAATRVADLEADLKTARHERDVFEEQLSQASNA 859

Query: 663 DSAQKVELCYLLDALQKKARIPSLEEEVTSLRTFDSAQKVELCYLLDALQKKARIASLEE 722
            +   V    L    +  ARI  LEEE+T  R   S  + EL  + D L++  R     +
Sbjct: 860 AATVAVAEPPLQTTKEADARIALLEEELTGTRQELSDVREELRQVQDELEQLLR-----D 919

Query: 723 EVTSPRTFDSAQKV--ELCYLLDALQKKAR---------IASLEEEVTSPRTFDSAQNVE 771
           +  S  T    ++V  EL + L  L + A          + +  +   S    D+   V 
Sbjct: 920 QEASDATIQEYEEVIQELEHKLARLDQPAEDTGRNQSEPVVNSNDIPESSVPADTTDLVS 964

BLAST of MC01g_new0236 vs. ExPASy TrEMBL
Match: A0A0Q5PWP0 (Uncharacterized protein OS=Microbacterium sp. Leaf179 OX=1736288 GN=ASF96_06595 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 9.42e-08
Identity = 68/266 (25.56%), Postives = 135/266 (50.75%), Query Frame = 0

Query: 583 TFDSAQKVELCYLLDALQKKARIPSLE-----EEVTSPRTFDSAQKVELCYLLDALQKKA 642
           +F+S + +E    L++ +    + SLE     E V S  +F+S + VE    +++L+   
Sbjct: 3   SFESVESLESVMSLESFESLESVESLESVESLESVMSLESFESQESVESLESVESLESVE 62

Query: 643 RIPSLE--EEVTSLRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELC 702
            + S+   E + S  + +S +  E    L++++ +  + SLE  V S  +F+S + VE  
Sbjct: 63  SLESVASCESLESFESLESVESQESVESLESVESQESVESLES-VMSLESFESQESVESL 122

Query: 703 YLLDA--LQKKARIASLEEEVTSPRTFDSAQNVELCYLLDALQKRAIIPSLEEASSLRTF 762
             +++  ++ +  + SLE  V S  +F+S ++VE    +++L+    + S E   S+ + 
Sbjct: 123 ESVESESVESQESVESLES-VMSLESFESQESVESLESVESLESVMSLESFESQESVESL 182

Query: 763 DSAQKVELCYLLDALQKRTTILSLVGEKMAIIPSLEEKVMSPRTFESAQKEELCYLLDAL 822
           +S + +E     ++L+   ++ S+  E    + SLE  VMS  +FES +  E    +++ 
Sbjct: 183 ESVESLESVESFESLESFESLESV--ESQESVESLES-VMSLESFESQESVESLESVES- 242

Query: 823 QKMAIIPS-----SLEKVMSLMSYGS 834
           + +  + S     SLE VMSL S+ S
Sbjct: 243 ESLESVESQESVESLESVMSLESFES 262

BLAST of MC01g_new0236 vs. ExPASy TrEMBL
Match: A0A6G1J0N3 (Uncharacterized protein OS=Lentithecium fluviatile CBS 122367 OX=1168545 GN=K458DRAFT_36958 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.32e-06
Identity = 152/676 (22.49%), Postives = 277/676 (40.98%), Query Frame = 0

Query: 66  EEVTSLRTFDSAQKVELCYLLDALQKK-----ARIPSLEEE--VTSPRTFDSAQKVELCY 125
           E+V  +++   AQK EL   +  L+ +     A I  LE E   + P+T   A++ E+  
Sbjct: 30  EKVEKIKSDFEAQKAELQGRIGELESRISELEATIARLESERGASVPKTDLEAKENEIAA 89

Query: 126 LL-DALQKRAIIPSLEEEVTSLRTFDSAQKVELCYFLDALQKKARIPSLEEEVTSPRTFD 185
           L  D       +     ++  L+  +++ + E      +L  KA + + EE +       
Sbjct: 90  LTSDKTTAEESLAKANADLEGLKAANASLEEEKA----SLVPKADLEAKEETIAEKTAAQ 149

Query: 186 SAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKA-------- 245
            A       + +    +A+IA+L++E+ + ++       +L    D   K +        
Sbjct: 150 EA------LMKEKEDLEAQIAALQKELDAEKSNHGETSEKLTTQADEFGKLSEEHTARGT 209

Query: 246 RIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYL 305
           RI  LE  + +  +  SA        L+ L K+      E+ VT       A   ++  L
Sbjct: 210 RISELEAAIATHES-SSADIASKVASLEGLIKEK-----EDAVTGKDAEIDAANAKVVEL 269

Query: 306 LDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSA 365
            ++   K+ +  LE+++ +    D+  ++E      AL++     S  E +T+ +T   A
Sbjct: 270 EES--SKSSLAELEQQLKAKT--DAVSELESAKE-SALKEAEEAKSSFEGLTNEKTALEA 329

Query: 366 QKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVT 425
              E    L A + +  +A  E E T       A+++         + K+ +A+ EE + 
Sbjct: 330 SLKEAKDNLSATESEIAVAKSEAEKTKGEASSLAEQIS--------ELKSSLATKEEALA 389

Query: 426 SPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARI 485
           S           L   +  L  K+ + + + E+        + K EL         +ARI
Sbjct: 390 SAEAAAKGDHEGLNAQIAEL--KSSLEAKDTELAEANKLAESSKGEL---------EARI 449

Query: 486 ASLEEEVTSPRT-FDSAQK----VELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVEL 545
           A LE+ + S +T  D+ Q+    VE     D+    A+I  LE  + +    + A K EL
Sbjct: 450 AELEKAIESKQTELDAKQEAIRAVEATAKGDSDSLGAKIAELETSLKAASEKEEAAKTEL 509

Query: 546 CYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTF 605
                    K+ + S EE + +  +F +          +A + +AR+  LE ++T+ +  
Sbjct: 510 ------ETAKSELASKEEALKASESFRT----------EAEEAQARVSKLEADLTAAKEL 569

Query: 606 DSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKV-EL-CYLLDALQKKARIPS- 665
           +S +  EL  +   L  K       E      +   A K+ EL   L +A +K+    S 
Sbjct: 570 ESTKDAELEGIKSELSSKEDALQAAEAAAKGDSESQAAKITELESSLKEATEKEQAAGSK 629

Query: 666 LEEEVTSLRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDAL 712
           LE   + L + D A K       +A + KARI++LE E+ + +   SA+  +L   LD  
Sbjct: 630 LETLKSDLDSKDEALKASESLKTEAEEAKARISTLEGELAAAKEA-SAKVADLQSSLDEA 648

BLAST of MC01g_new0236 vs. ExPASy TrEMBL
Match: A0A7S0G8E9 (Hypothetical protein (Fragment) OS=Rhodosorus marinus OX=101924 GN=RMAR0315_LOCUS11605 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.62e-05
Identity = 96/357 (26.89%), Postives = 164/357 (45.94%), Query Frame = 0

Query: 63  SLEEEVTSLRTFDSAQKVELCYLLDAL-QKKARIPSLEEEVTS----PRTFDSAQKVELC 122
           +L+E V++L      Q+  L  L   L +K ARI  L+ ++       +  +SA++ +L 
Sbjct: 176 TLKERVSTLEEDGRIQRDTLATLEQELSEKNARIADLQRDIEKVEKVEKVDNSAEQEKLV 235

Query: 123 YLLDAL-QKRAIIPSLEEEVTSLRTFDSAQKVELCYFLDALQKKARIPSLE---EEVTSP 182
           +L   L +K   I  L+ E+ +     S  K+E     +  +K A+I SL+   EE+   
Sbjct: 236 FLEQELSEKSRRIEILQMELENATATASGGKLEALEE-ELSEKNAKIASLQTKIEELDEK 295

Query: 183 RTFDSAQKVELCYLLDALQKKARIASLEEEVTS----PRTFDSAQKVELCYLLDALQKKA 242
              D   ++E     +  +K ARIASLE EV S     + F  A++    +L D   + A
Sbjct: 296 TGLDKLAELEQ----EINEKNARIASLEVEVESYGGSSKKFRPAEQ----HLSDTDTRTA 355

Query: 243 RIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIPSLEEEVTSPRTFDSAQKVELCYL 302
            + S  E+       D    +E     +  +K+AR+ S+E E+ +    DSA++ +L + 
Sbjct: 356 SLGSELEKADGRTQNDGLDALEQ----ELSEKRARLESVEMELGT--VGDSARRDKLAFE 415

Query: 303 LDALQKKARIPSLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEEEVTSPRTFDSA 362
            +  +K A I SLE E+ + R  +S Q          ++K++   S+E  V  P   ++A
Sbjct: 416 QEMSEKNATIASLETEIKNMR--ESEQ----------VEKRSVGTSIESNV--PEVVETA 475

Query: 363 QKVELCYLLDALQKKARIASLEEEVTSPRTFDSAQKVELCYLLDALQKKARIASLEE 406
             +E+    D    +A I  L EE    ++ D  +KVE        +    IASLE+
Sbjct: 476 TGIEVTSKTDT--SEALIERLAEE--KQKSLDGERKVE--------ELNTYIASLEQ 491