MC01g_new0210 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0210
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Retrovirus-related Pol polyprotein from transposon RE1
Location	MC01: 7646938 .. 7651162 (+)
RNA-Seq Expression	MC01g_new0210
Synteny	MC01g_new0210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCATTTTTAACCGCCGTGTACCTCATAAATAGAATGCCTACAAAAGCCTTAAATATGAACTCTCCTTTCTAAAAGCTATATGGGAGAACTCCTAATTACAATAATATGAGAATATTTGATTGTCGATGTTTTCCTTACTTACAAAGCAAACCAAATAACAAGTTCCCTAAACGAACATATCATTGTGTCTTTGTCGGTTATATTATGGACCATAAGGGATATAGATGTCTTGACCCTTCGACCAATCGCATATATATCTCAAGGCATGTAGTGTTCGATGAAGAATTTTTTCCCTTTAATCAAACCATAGCTAACAACTCTATCAAGAGTATTTCTCCTCTTGTTGTTACTACTTTTCAGGCACAATGAATGAAGATGCAGCCTAAACTCAAGCATCTGTAAAGTCACTTGAAAACAAGACTCAAGCCACAAATTAAGAAGACCAACCTGCAGATACCAATTCATGACAAGCAGCAAAAATTAGCTACTCAACTACCATTGTGGCAAGTCTAGATGTTGTACCAGCAACAATAACAAATCAACACAATGCTGCAACAAGGAACTCAAATATACCAGCAGTTGAAGGAGATAAAGAAGACCAAAATCATTTCAACAATACATCTACAGTAGTAGCAAAAATCAGTAGCTCAATTGAAGGTGATTTCAACAATACATCTACAATAGTAGCAAAAATCAGTAGCTCAATTGAAGGAGTTCAAGAGGACCAAAATCACCCCTGTAGTAGCTCAATTCAAACACAACAGTTTGGTTTGCAACCCGATTCACGAAGCCATCTCAGTGAATCAAATACAAAAGAAGTAGCAACTGGTGAAATCAATGCATCAGATTTGACTTCCTCTCTCCTTACTAATTCTTCTCCTTGTCCTACAAATGCTGATTTTAGTTATTTTCAAGATATTGGTGACTTAGTTCATATTGATTTGAATTTTTCACCTGCAGAAACATTACCAAAAAATCAAAGCAACCAATTGGGCCAATCTTATATCCCCTCTACTCATCAAATGGTTATATGAAGAAAACTAGCTGAGAATCCATCCCTTGGTACAAACTTAGCTCAAGAAGTCCATCACTTGAAAGCTAAGAAATATCACCAATCTTATTTGGCTTCAAGTTCTCAAATAGAACCAAAGAACTACAAAATAGTTTTGATCCAACCTCATTAGCAAAGTGCAATGAAGGATGAAATGAAGGCACTCCTACAAAACCATACTTGGACCCTTGTTCCTAGACCTCATGACTCCAACATAGTAGGATCCAAGTGGGTTTTCAAAATAAAGCACAAGGAAGATGGCACCATCGATCGTTAGAAAGCACGACTAGTAGCCCAAGGTTACTCTCAAATAGAAGGACTCGACTACGGAGAAACCTATAGCCTTGTGATCAAACCTACTACAATAAGACTAATCATATCCCTAGCGACTAGTTTAAGTTGGCCCATACAACAATTAGATGTTAAAAATACTTTTCTCCATGGTCACCTTAAAGAAAAGGTTTATATGGCGCCACCCCCGGTTTTGTGGATCCAAGTTATCCTACTCATGTATGTAAGTTGGAAAAATCTATTTATGGGTTAAAATAAGCCCTTAGAGCTTGGTTTGATAGATTGGCTAACTTTCTTCTCCATACGGGATTTACTTGTAGCAGATCAGATCCCTCCTTATTTATATTGAAAAAGGACTATATTCTAACGCTTATGTTAATTTATGTTGACGACATTATTCTTACAGGGCATGATATTTCTCACATTCAATAGTTAATACAAACACTAAGTGGTGAGTTTGGGTTAAAAGATCTTGGTGTCCTACATTACTTCCTTGTAATTGAAGTCAAGCATACTCATAAAGGAATCACATTATGCCAAGGAAGATATGCTCGTGTTCTTCTTTGCAAATCCAATATGCTTGGTGCATCCTCCATAAGCAAACCAATGGCCATATCAACACAGGTCTTGCCCAATGATGAGCAACCCATAGATGTCAAACAATATAGAAGCCTAGTAGGATCTCTTCAATACCTCACTCTCACCCGTCCTGACATCGTACAAGCTGTGAATAAAGTGTGCCAACGACTCCAAAATCCCACCATAGCAGACCTCAAAGTTGTGAAGCGAATCCTACGATACATTCAAGGAACGGTAGATCATGGTATTACATTTTATAAACGTAGCACAACTAACTTATATGGTTTTTGTGATGCTAATTGGAAATGGTGTCCTCTGACAAGATGGAGTACCACTGGATATTGTATATTTCTTGGTTCAAACTGCATCTCATGGTCTTCCAAGAAACAATCGACCATCGCTCGTTCAAGTTCTGAAGCAGAATATCGAGCAATGGCTAATGCAACTACCGAACTGACATAAATTGGCTTCCTCCTTCGCGACATTGTCATCCTAATACTTAAAACACCTTAACTATATTGTGATAACATGAGTGCTCTCTACATGTCAATAAATCCCGTGTTCCATGCTAGGACCAAACACATTGAGATGGATTATCATTTTGTTCGTGAGAAGGTTGCTCTCAGCTCTCTCATCACCAAGTTCGTTCCATCCAAACAACAAATAGCTGACATCCTCACTAAACCCCTCGCTAAAGCTGTTTTTAAAGACTTAAGGAGCAAACTCGACGTTCGTTCAACCACTACCTTGAGTTCGATAGACGATGATAAACAACAAAATGATACACCAGCAAAATACAACAAAATAACACACCAGTAGGATATATTAACACCTCAGCAAGTGATATTTCAAAATAGCAGCAGTAGATCTAAGTCCTTAATTGGCATCATTACATGTTCAACATATTCTCCTTCCTTATTTGGTGAAGATTTTAGGTAATCTACTACAACTCAATTACTCAGCAATATCCCTTAATTGCTAGCCTTTATTCATTTCTTATTACTTTGCAACCTTCCTTACCTATTTAATTTTTCCTTTCTTGCTTTAGCTTGGTCAATGGTAGTCTTTCATGTGTCTATTTATCCATCTTGGATTTAGCTTGTAAAACATCTTTTTTCACACAAACCAGAATAATACATCATCTGTTTTCATTGTTCCTTCAATTCTATTTCTTTAACCCATTCTTTCAAGCTAAGTTTAATAGTTACAACATCCCTTTCTAATTTGCTTAGAATAGTTTGCCATTTCTTCCAAATAAAATAAAAAGTGTCATTCAGGGATCAAGGAGAAGCTGAGCTGAGGGGAGGATAGCTTGCTAGATAGCTTGAGCCCGAGAGCAAAAGAAGAGGAAATGCATAACAGTTTCAAAATTATTGTGACAGCAATGACAATAACGTGATATATCCATACATTTTCTCGGTAGGTTGTCTCAAGGATCCCACATTGAATAGAAAAGAGACCGAACAAAGGTTTATAATAGACATGGACTAGTCCTCTCATTGCCAATTGATTTTGAGATGGAACCCCATGAATATAAATAGCACATGGTAGGAAGATACCCATGGTTTGCTTTCCAAATAAAAGATTTTGAGATGGATACCCATGGTAGGAAGATACTCATTACCAATTGATTTTGAGATGGATACCCATGAATATGAATAGCACATGATAGAACTGCTAGCTTTTGAGATTAACTTTAGATTCATATATAGCTTGTATGAACTTTCACACTAAATGAGCCCCTTTTATCAAAATGCCACATCATCAAGGAGATTACGACAAGTAAAACTAATTGTTAGAATGAGGTTGATATCTTTTTGAGGTAGAAAATCTGATAATCTTTGAATATCCCAAGTGCATAGAGGACGAATGAAGTGAGAAACTTGAATGACCTTATGAGTGTTAGGAGGGGAGAGATGTCTAAAAGACCACTCTCTAGGCAGCCAAGGATCATGGAACACTCGGATCGAGCCTCCATTTCCAGCCTACTTTCTAATTCCTTCAATTAGAAGTTCACGACTCCAAAACAAACTTTTTCAAATATAGGAAAACTACGAGTGAGATTTTGTGTCTAGAGTAAATGAATCCAGGTTATTTTGGCCCACAGCACCCTAAAGACTAGAAGGTTTGGTTTTTGAAGAAATCTCCAGACTTATTTGGCTAGGAGAGCTTTATTGAGACCCTTCAAGTCAAGAAAGTTAAGACCCCAAGCTCTTTAGGTAAGCACATGCACTTTCAACTCATCCAATGAAGCTTCTTCTATTTGCTAATGGATCCTCACCAAAAGCGGGTGAGAGTTTCAGA

mRNA sequence

ATGCTTGGTGCATCCTCCATAAGCAAACCAATGGCCATATCAACACAGGTCTTGCCCAATGATGAGCAACCCATAGATGTCAAACAATATAGAAGCCTAGTAGGATCTCTTCAATACCTCACTCTCACCCGTCCTGACATCGTACAAGCTGTGAATAAAGTGTGCCAACGACTCCAAAATCCCACCATAGCAGACCTCAAAGTTGTGAAGCGAATCCTACGATACATTCAAGGAACGGTAGATCATGGTATTACATTTTATAAACGTAGCACAACTAACTTATATGGTTTTTGTGATGCTAATTGGAAATGGTGTCCTCTGACAAGATGGAGTACCACTGGATATTGTATATTTCTTGGTTCAAACTGCATCTCATGGTCTTCCAAGAAACAATCGACCATCGCTCGTTCAAGTTCTGAAGCAGAATATCGAGCAATGGCTAATGCAACTACCGAACTGACATAA

Coding sequence (CDS)

ATGCTTGGTGCATCCTCCATAAGCAAACCAATGGCCATATCAACACAGGTCTTGCCCAATGATGAGCAACCCATAGATGTCAAACAATATAGAAGCCTAGTAGGATCTCTTCAATACCTCACTCTCACCCGTCCTGACATCGTACAAGCTGTGAATAAAGTGTGCCAACGACTCCAAAATCCCACCATAGCAGACCTCAAAGTTGTGAAGCGAATCCTACGATACATTCAAGGAACGGTAGATCATGGTATTACATTTTATAAACGTAGCACAACTAACTTATATGGTTTTTGTGATGCTAATTGGAAATGGTGTCCTCTGACAAGATGGAGTACCACTGGATATTGTATATTTCTTGGTTCAAACTGCATCTCATGGTCTTCCAAGAAACAATCGACCATCGCTCGTTCAAGTTCTGAAGCAGAATATCGAGCAATGGCTAATGCAACTACCGAACTGACATAA

Protein sequence

MLGASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQNPTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLGSNCISWSSKKQSTIARSSSEAEYRAMANATTELT

Homology

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BLAST of MC01g_new0210 vs. ExPASy Swiss-Prot
Match: P92519 (Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 GN=AtMg00810 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 4.8e-39
Identity = 78/154 (50.65%), Postives = 106/154 (68.83%), Query Frame = 0


Query: 1   MLGASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQN 60
           ML    +S P+ +      +  +  D   +RS+VG+LQYLTLTRPDI  AVN VCQR+  
Sbjct: 71  MLDCKPMSTPLPLKLNSSVSTAKYPDPSDFRSIVGALQYLTLTRPDISYAVNIVCQRMHE 130

Query: 61  PTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLG 120
           PT+AD  ++KR+LRY++GT+ HG+  +K S  N+  FCD++W  C  TR STTG+C FLG
Sbjct: 131 PTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCTFLG 190

Query: 121 SNCISWSSKKQSTIARSSSEAEYRAMANATTELT 155
            N ISWS+K+Q T++RSS+E EYRA+A    ELT
Sbjct: 191 CNIISWSAKRQPTVSRSSTETEYRALALTAAELT 224

[Close]

BLAST of MC01g_new0210 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-29
Identity = 71/154 (46.10%), Postives = 102/154 (66.23%), Query Frame = 0


Query: 1    MLGASSISKPMAISTQV-LPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQ 60
            M+ A  ++ PMA S ++ L +  +  D  +YR +VGSLQYL  TRPDI  AVN++ Q + 
Sbjct: 1215 MITAKPVTTPMAPSPKLSLYSGTKLTDPTEYRGIVGSLQYLAFTRPDISYAVNRLSQFMH 1274

Query: 61   NPTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFL 120
             PT   L+ +KRILRY+ GT +HGI   K +T +L+ + DA+W        ST GY ++L
Sbjct: 1275 MPTEEHLQALKRILRYLAGTPNHGIFLKKGNTLSLHAYSDADWAGDKDDYVSTNGYIVYL 1334

Query: 121  GSNCISWSSKKQSTIARSSSEAEYRAMANATTEL 154
            G + ISWSSKKQ  + RSS+EAEYR++AN ++E+
Sbjct: 1335 GHHPISWSSKKQKGVVRSSTEAEYRSVANTSSEM 1368

[Close]

BLAST of MC01g_new0210 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-29
Identity = 71/154 (46.10%), Postives = 100/154 (64.94%), Query Frame = 0


Query: 1    MLGASSISKPMAISTQV-LPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQ 60
            ML A  ++ PMA S ++ L +  +  D  +YR +VGSLQYL  TRPD+  AVN++ Q + 
Sbjct: 1198 MLTAKPVATPMATSPKLTLHSGTKLPDPTEYRGIVGSLQYLAFTRPDLSYAVNRLSQYMH 1257

Query: 61   NPTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFL 120
             PT      +KR+LRY+ GT DHGI   K +T +L+ + DA+W        ST GY ++L
Sbjct: 1258 MPTDDHWNALKRVLRYLAGTPDHGIFLKKGNTLSLHAYSDADWAGDTDDYVSTNGYIVYL 1317

Query: 121  GSNCISWSSKKQSTIARSSSEAEYRAMANATTEL 154
            G + ISWSSKKQ  + RSS+EAEYR++AN ++EL
Sbjct: 1318 GHHPISWSSKKQKGVVRSSTEAEYRSVANTSSEL 1351

[Close]

BLAST of MC01g_new0210 vs. ExPASy Swiss-Prot
Match: P0CV72 (Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.3e-20
Identity = 53/124 (42.74%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 30  YRSLVGSLQYL-TLTRPDIVQAVNKVCQRLQNPTIADLKVVKRILRYIQGTVDHGITFYK 89
           Y S VG++ YL  +TRPD+  AV  + Q   +P     + +KR+LRY+Q T  +G+ F +
Sbjct: 6   YLSAVGAIMYLMVVTRPDLAAAVGVLSQFASDPCPTHWQALKRVLRYLQSTQTYGLEFTR 65

Query: 90  RSTTNLYGFCDANWKWCPLTRWSTTGYCIFLGSNCISWSSKKQSTIARSSSEAEYRAMAN 149
             T  L G+ DA+W     +R ST+GY   L   C+SW SKKQ T+A SS+E EY A++ 
Sbjct: 66  AGTAKLVGYSDADWAGDVESRRSTSGYLFKLNGGCVSWRSKKQRTVALSSTEDEYMALSE 125

Query: 150 ATTE 153
           AT E
Sbjct: 126 ATQE 129

[Close]

BLAST of MC01g_new0210 vs. ExPASy Swiss-Prot
Match: P93290 (Uncharacterized mitochondrial protein AtMg00240 OS=Arabidopsis thaliana OX=3702 GN=AtMg00240 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.5e-11
Identity = 32/78 (41.03%), Postives = 47/78 (60.26%), Query Frame = 0


Query: 39  YLTLTRPDIVQAVNKVCQRLQNPTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFC 98
           YLT+TRPD+  AVN++ Q       A ++ V ++L Y++GTV  G+ +   S   L  F 
Sbjct: 2   YLTITRPDLTFAVNRLSQFSSASRTAQMQAVYKVLHYVKGTVGQGLFYSATSDLQLKAFA 61

Query: 99  DANWKWCPLTRWSTTGYC 117
           D++W  CP TR S TG+C
Sbjct: 62  DSDWASCPDTRRSVTGFC 79

[Close]

BLAST of MC01g_new0210 vs. NCBI nr
Match: XP_022141787.1 (uncharacterized protein LOC111012071 [Momordica charantia])

HSP 1 Score: 202 bits (514), Expect = 1.13e-61
Identity = 101/144 (70.14%), Postives = 115/144 (79.86%), Query Frame = 0


Query: 11  MAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQNPTIADLKVVK 70
           MA+S+   PND   +D   YRS+VGSLQYLTLTRPDIV AVNKVCQ +Q  TI + + VK
Sbjct: 144 MALSSTSSPNDGDLVDATTYRSIVGSLQYLTLTRPDIVYAVNKVCQHMQTTTINNFRDVK 203

Query: 71  RILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLGSNCISWSSKK 130
           RILRY++GT+ HGITF+K S  NLY FCDA+W  CP TR STTGYCIFL +NCISWSSKK
Sbjct: 204 RILRYLKGTLSHGITFHKNSNLNLYAFCDADWGGCPSTRRSTTGYCIFLEANCISWSSKK 263

Query: 131 QSTIARSSSEAEYRAMANATTELT 154
           Q TIARSS+EAEYRAMAN T ELT
Sbjct: 264 QPTIARSSTEAEYRAMANTTAELT 287

[Close]

BLAST of MC01g_new0210 vs. NCBI nr
Match: XP_022157629.1 (uncharacterized protein LOC111024290 [Momordica charantia])

HSP 1 Score: 202 bits (513), Expect = 1.16e-61
Identity = 98/151 (64.90%), Postives = 118/151 (78.15%), Query Frame = 0


Query: 4   ASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQNPTI 63
           AS  + PMA++    PND++P+D   YRS+VGSLQYL+LTRPDI+QAVNK+ Q  Q+PT+
Sbjct: 122 ASISATPMALAPPSSPNDDEPVDTTSYRSIVGSLQYLSLTRPDIIQAVNKIYQHFQSPTV 181

Query: 64  ADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLGSNC 123
            DLK VKRILRY+ GT   GIT YK  T NLY FCD +W  CP+TR ST G+C++LGSN 
Sbjct: 182 KDLKEVKRILRYLVGTTHLGITIYKNHTLNLYAFCDTDWGGCPVTRQSTIGFCVYLGSNI 241

Query: 124 ISWSSKKQSTIARSSSEAEYRAMANATTELT 154
           ISWSSKKQ TIARSSSE+EYRAMA  TTE+T
Sbjct: 242 ISWSSKKQPTIARSSSESEYRAMATTTTEIT 272

[Close]

BLAST of MC01g_new0210 vs. NCBI nr
Match: XP_022139305.1 (uncharacterized protein LOC111010253 isoform X1 [Momordica charantia])

HSP 1 Score: 200 bits (508), Expect = 2.16e-60
Identity = 102/154 (66.23%), Postives = 118/154 (76.62%), Query Frame = 0


Query: 1   MLGASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQN 60
           ML AS +S PM+ ST    ND + ID  QYRSLVGSLQYLTLTRPDI  AVNK CQ L +
Sbjct: 69  MLEASQLSTPMSTSTSSSSNDNEIIDATQYRSLVGSLQYLTLTRPDITFAVNKACQHLHD 128

Query: 61  PTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLG 120
           P   DL +VK ILRY++GT+ +GI+FYK S+ NLY FCDA+W  CP TR ST+G CIFLG
Sbjct: 129 PKQKDLNMVKHILRYLKGTLRYGISFYKNSSLNLYAFCDADWGGCPDTRRSTSGCCIFLG 188

Query: 121 SNCISWSSKKQSTIARSSSEAEYRAMANATTELT 154
           SNCI WSSKKQ+T+ARSSSEAEYRAMA    +LT
Sbjct: 189 SNCIFWSSKKQTTVARSSSEAEYRAMATIAADLT 222

[Close]

BLAST of MC01g_new0210 vs. NCBI nr
Match: RVW95106.1 (Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera])

HSP 1 Score: 188 bits (478), Expect = 1.00e-56
Identity = 92/154 (59.74%), Postives = 117/154 (75.97%), Query Frame = 0


Query: 1   MLGASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQN 60
           M+  + I+ P+ + + +   DEQPID  QYR LVGSLQYLT TRPDIV AVNK CQ  Q 
Sbjct: 1   MMECTHINTPVDLKSIITLFDEQPIDPTQYRQLVGSLQYLTFTRPDIVHAVNKACQYFQE 60

Query: 61  PTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLG 120
           PT ADL+VVK ILRY++GT++HGI F+K+S+  L  FCDA+W  C   R ST+GYCIFLG
Sbjct: 61  PTKADLRVVKHILRYLKGTMEHGIRFFKQSSLRLTSFCDADWAGCTNIRRSTSGYCIFLG 120

Query: 121 SNCISWSSKKQSTIARSSSEAEYRAMANATTELT 154
           +NCISWSSK+Q T++RSS+EAEYR  A++  E+T
Sbjct: 121 ANCISWSSKRQPTVSRSSAEAEYRYFASSAVEIT 154

[Close]

BLAST of MC01g_new0210 vs. NCBI nr
Match: RVW60851.1 (Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera])

HSP 1 Score: 188 bits (478), Expect = 1.09e-56
Identity = 91/148 (61.49%), Postives = 115/148 (77.70%), Query Frame = 0


Query: 7   ISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQNPTIADL 66
           I+ PMA+ + + P DEQPID  +YR LVGSLQYLT TRPDIV AVNK CQ  Q PT ADL
Sbjct: 6   INTPMALKSIITPFDEQPIDPTRYRQLVGSLQYLTFTRPDIVHAVNKACQHFQAPTKADL 65

Query: 67  KVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLGSNCISW 126
           + VK ILRY++ T++HGI F+K+S+  L GFCD +W  C  TR ST+GYCIFLG+NCISW
Sbjct: 66  RAVKLILRYLKRTMEHGINFFKQSSLRLTGFCDVDWVGCTNTRRSTSGYCIFLGANCISW 125

Query: 127 SSKKQSTIARSSSEAEYRAMANATTELT 154
           SSK+QST +RSS EAEYR++ ++  ++T
Sbjct: 126 SSKRQSTFSRSSVEAEYRSLESSAAKIT 153

[Close]

BLAST of MC01g_new0210 vs. ExPASy TrEMBL
Match: A0A6J1CKU6 (uncharacterized protein LOC111012071 OS=Momordica charantia OX=3673 GN=LOC111012071 PE=4 SV=1)

HSP 1 Score: 202 bits (514), Expect = 5.46e-62
Identity = 101/144 (70.14%), Postives = 115/144 (79.86%), Query Frame = 0


Query: 11  MAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQNPTIADLKVVK 70
           MA+S+   PND   +D   YRS+VGSLQYLTLTRPDIV AVNKVCQ +Q  TI + + VK
Sbjct: 144 MALSSTSSPNDGDLVDATTYRSIVGSLQYLTLTRPDIVYAVNKVCQHMQTTTINNFRDVK 203

Query: 71  RILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLGSNCISWSSKK 130
           RILRY++GT+ HGITF+K S  NLY FCDA+W  CP TR STTGYCIFL +NCISWSSKK
Sbjct: 204 RILRYLKGTLSHGITFHKNSNLNLYAFCDADWGGCPSTRRSTTGYCIFLEANCISWSSKK 263

Query: 131 QSTIARSSSEAEYRAMANATTELT 154
           Q TIARSS+EAEYRAMAN T ELT
Sbjct: 264 QPTIARSSTEAEYRAMANTTAELT 287

[Close]

BLAST of MC01g_new0210 vs. ExPASy TrEMBL
Match: A0A6J1DX10 (uncharacterized protein LOC111024290 OS=Momordica charantia OX=3673 GN=LOC111024290 PE=4 SV=1)

HSP 1 Score: 202 bits (513), Expect = 5.62e-62
Identity = 98/151 (64.90%), Postives = 118/151 (78.15%), Query Frame = 0


Query: 4   ASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQNPTI 63
           AS  + PMA++    PND++P+D   YRS+VGSLQYL+LTRPDI+QAVNK+ Q  Q+PT+
Sbjct: 122 ASISATPMALAPPSSPNDDEPVDTTSYRSIVGSLQYLSLTRPDIIQAVNKIYQHFQSPTV 181

Query: 64  ADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLGSNC 123
            DLK VKRILRY+ GT   GIT YK  T NLY FCD +W  CP+TR ST G+C++LGSN 
Sbjct: 182 KDLKEVKRILRYLVGTTHLGITIYKNHTLNLYAFCDTDWGGCPVTRQSTIGFCVYLGSNI 241

Query: 124 ISWSSKKQSTIARSSSEAEYRAMANATTELT 154
           ISWSSKKQ TIARSSSE+EYRAMA  TTE+T
Sbjct: 242 ISWSSKKQPTIARSSSESEYRAMATTTTEIT 272

[Close]

BLAST of MC01g_new0210 vs. ExPASy TrEMBL
Match: A0A1S8ACR3 (DNA/RNA polymerases superfamily protein (Fragment) OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 198 bits (504), Expect = 1.08e-61
Identity = 94/154 (61.04%), Postives = 123/154 (79.87%), Query Frame = 0


Query: 1   MLGASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQN 60
           ML ++S+S PMAI  Q  P D +P++  +YR LVG LQYLTLTRPDIV AVNKVCQ  Q 
Sbjct: 1   MLESTSLSTPMAIKEQANPIDHKPVNATEYRRLVGGLQYLTLTRPDIVHAVNKVCQHFQT 60

Query: 61  PTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLG 120
           PT A+L+ VKRILRY++G++D+GI F ++S+ N+  FCDA+W  CP TR STTGYC+++G
Sbjct: 61  PTEANLRAVKRILRYLKGSLDYGIRFLQQSSLNITAFCDADWGGCPDTRRSTTGYCVYMG 120

Query: 121 SNCISWSSKKQSTIARSSSEAEYRAMANATTELT 154
           +NCISWSSK+Q T++RSS+EAEYRA+A+   ELT
Sbjct: 121 ANCISWSSKRQPTVSRSSAEAEYRALASTAAELT 154

[Close]

BLAST of MC01g_new0210 vs. ExPASy TrEMBL
Match: A0A6J1CBZ1 (uncharacterized protein LOC111010253 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010253 PE=4 SV=1)

HSP 1 Score: 200 bits (508), Expect = 1.05e-60
Identity = 102/154 (66.23%), Postives = 118/154 (76.62%), Query Frame = 0


Query: 1   MLGASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQN 60
           ML AS +S PM+ ST    ND + ID  QYRSLVGSLQYLTLTRPDI  AVNK CQ L +
Sbjct: 69  MLEASQLSTPMSTSTSSSSNDNEIIDATQYRSLVGSLQYLTLTRPDITFAVNKACQHLHD 128

Query: 61  PTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLG 120
           P   DL +VK ILRY++GT+ +GI+FYK S+ NLY FCDA+W  CP TR ST+G CIFLG
Sbjct: 129 PKQKDLNMVKHILRYLKGTLRYGISFYKNSSLNLYAFCDADWGGCPDTRRSTSGCCIFLG 188

Query: 121 SNCISWSSKKQSTIARSSSEAEYRAMANATTELT 154
           SNCI WSSKKQ+T+ARSSSEAEYRAMA    +LT
Sbjct: 189 SNCIFWSSKKQTTVARSSSEAEYRAMATIAADLT 222

[Close]

BLAST of MC01g_new0210 vs. ExPASy TrEMBL
Match: A0A438IED0 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=29760 GN=RE1_28 PE=4 SV=1)

HSP 1 Score: 188 bits (478), Expect = 4.85e-57
Identity = 92/154 (59.74%), Postives = 117/154 (75.97%), Query Frame = 0


Query: 1   MLGASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQN 60
           M+  + I+ P+ + + +   DEQPID  QYR LVGSLQYLT TRPDIV AVNK CQ  Q 
Sbjct: 1   MMECTHINTPVDLKSIITLFDEQPIDPTQYRQLVGSLQYLTFTRPDIVHAVNKACQYFQE 60

Query: 61  PTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLG 120
           PT ADL+VVK ILRY++GT++HGI F+K+S+  L  FCDA+W  C   R ST+GYCIFLG
Sbjct: 61  PTKADLRVVKHILRYLKGTMEHGIRFFKQSSLRLTSFCDADWAGCTNIRRSTSGYCIFLG 120

Query: 121 SNCISWSSKKQSTIARSSSEAEYRAMANATTELT 154
           +NCISWSSK+Q T++RSS+EAEYR  A++  E+T
Sbjct: 121 ANCISWSSKRQPTVSRSSAEAEYRYFASSAVEIT 154

[Close]

BLAST of MC01g_new0210 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 3.4e-40
Identity = 78/154 (50.65%), Postives = 106/154 (68.83%), Query Frame = 0


Query: 1   MLGASSISKPMAISTQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQN 60
           ML    +S P+ +      +  +  D   +RS+VG+LQYLTLTRPDI  AVN VCQR+  
Sbjct: 71  MLDCKPMSTPLPLKLNSSVSTAKYPDPSDFRSIVGALQYLTLTRPDISYAVNIVCQRMHE 130

Query: 61  PTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFLG 120
           PT+AD  ++KR+LRY++GT+ HG+  +K S  N+  FCD++W  C  TR STTG+C FLG
Sbjct: 131 PTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCTFLG 190

Query: 121 SNCISWSSKKQSTIARSSSEAEYRAMANATTELT 155
            N ISWS+K+Q T++RSS+E EYRA+A    ELT
Sbjct: 191 CNIISWSAKRQPTVSRSSTETEYRALALTAAELT 224

[Close]

BLAST of MC01g_new0210 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 121.3 bits (303), Expect = 6.7e-28
Identity = 69/154 (44.81%), Postives = 97/154 (62.99%), Query Frame = 0


Query: 1   MLGASSISKPMAIS-TQVLPNDEQPIDVKQYRSLVGSLQYLTLTRPDIVQAVNKVCQRLQ 60
           +LG    S PM  S T    +    +D K YR L+G L YL +TR DI  AVNK+ Q  +
Sbjct: 348 LLGCKPSSVPMDPSVTFSAHSGGDFVDAKAYRRLIGRLMYLQITRLDISFAVNKLSQFSE 407

Query: 61  NPTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFCDANWKWCPLTRWSTTGYCIFL 120
            P +A  + V +IL YI+GTV  G+ +  ++   L  F DA+++ C  TR ST GYC+FL
Sbjct: 408 APRLAHQQAVMKILHYIKGTVGQGLFYSSQAEMQLQVFSDASFQSCKDTRRSTNGYCMFL 467

Query: 121 GSNCISWSSKKQSTIARSSSEAEYRAMANATTEL 154
           G++ ISW SKKQ  +++SS+EAEYRA++ AT E+
Sbjct: 468 GTSLISWKSKKQQVVSKSSAEAEYRALSFATDEM 501

[Close]

BLAST of MC01g_new0210 vs. TAIR 10
Match: ATMG00240.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 70.1 bits (170), Expect = 1.8e-12
Identity = 32/78 (41.03%), Postives = 47/78 (60.26%), Query Frame = 0


Query: 39  YLTLTRPDIVQAVNKVCQRLQNPTIADLKVVKRILRYIQGTVDHGITFYKRSTTNLYGFC 98
           YLT+TRPD+  AVN++ Q       A ++ V ++L Y++GTV  G+ +   S   L  F 
Sbjct: 2   YLTITRPDLTFAVNRLSQFSSASRTAQMQAVYKVLHYVKGTVGQGLFYSATSDLQLKAFA 61

Query: 99  DANWKWCPLTRWSTTGYC 117
           D++W  CP TR S TG+C
Sbjct: 62  DSDWASCPDTRRSVTGFC 79

The following BLAST results are available for this feature:

BLAST of MC01g_new0210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P92519	4.8e-39	50.65	Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q94HW2	1.6e-29	46.10	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
Q9ZT94	1.6e-29	46.10	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]
P0CV72	2.3e-20	42.74	Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 P...	[more]
P93290	2.5e-11	41.03	Uncharacterized mitochondrial protein AtMg00240 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of MC01g_new0210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141787.1	1.13e-61	70.14	uncharacterized protein LOC111012071 [Momordica charantia]	[more]
XP_022157629.1	1.16e-61	64.90	uncharacterized protein LOC111024290 [Momordica charantia]	[more]
XP_022139305.1	2.16e-60	66.23	uncharacterized protein LOC111010253 isoform X1 [Momordica charantia]	[more]
RVW95106.1	1.00e-56	59.74	Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera]	[more]
RVW60851.1	1.09e-56	61.49	Retrovirus-related Pol polyprotein from transposon RE2 [Vitis vinifera]	[more]

BLAST of MC01g_new0210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKU6	5.46e-62	70.14	uncharacterized protein LOC111012071 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1DX10	5.62e-62	64.90	uncharacterized protein LOC111024290 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A1S8ACR3	1.08e-61	61.04	DNA/RNA polymerases superfamily protein (Fragment) OS=Citrus limon OX=2708 PE=4 ...	[more]
A0A6J1CBZ1	1.05e-60	66.23	uncharacterized protein LOC111010253 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A438IED0	4.85e-57	59.74	Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=2976...	[more]

BLAST of MC01g_new0210 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00810.1	3.4e-40	50.65	DNA/RNA polymerases superfamily protein	[more]
AT4G23160.1	6.7e-28	44.81	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]
ATMG00240.1	1.8e-12	41.03	Gag-Pol-related retrotransposon family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11439:SF268	ATP-DEPENDENT DNA HELICASE	coord: 31..152
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 31..152
None	No IPR available	CDD	cd09272	RNase_HI_RT_Ty1	coord: 95..153 e-value: 1.90352E-29 score: 102.932
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 20..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g_new0210.1	MC01g_new0210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding