MC01g_new0209 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0209
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	polygalacturonase inhibitor-like
Location	MC01: 7593000 .. 7594498 (+)
RNA-Seq Expression	MC01g_new0209
Synteny	MC01g_new0209

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGAACTAATAAAAAATCTAGAAATTCATAAATCGAACCTATAATCTAACCTCTAAATCTAGTTAAAATTTAATAATGCATCCCTAACTTTGTTGTTTCCTCGAGCTACCATTCTTTAATAATTACCAAACTGTTTATAGTTTAATTGTTACGATGAAAAGATTGGAAGTTCGAATCCTCCCAAGAAAATCCCTTTGAACGGGCACGTGCAGCTGCCTATATAAGTTAAGAACTCTTCACGTTTTAGATTTAGCCAAAAACCTACCAAGGCCACATAGTAGTAGCCAGCCATGGCTGCTCCAAAGCTCACCTTCTTCTTCCTCCTCTTCATCTCCTCCTCCATTTGTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGTCCTCCTCAAAATCAAAAAATCCTTCAACAATCCCTACATCCTCACCTCATGGAAACCCGAAGAAGACTGCTGCAGTTGGTACTGTGTCCAATGTCATCCTACTTCTCACCGTATCATCTCCCTCACCATCTTCGCGGACGACAAACTCTCCGGCCAAATCCCCCCGGTGGTCGGCGATCTCCCGTTCCTCGAAACCCTGATGCTCCACAAGCTCCCCAATCTCACCGGCCCCATCCCCGCCGCCGTCGCCAATCTCCACAACCTCAAATCCCTCGACCTCAGCTGGAACGGCCTCTCCGGTCCGGTCCCGGAGTTCCTCGGCTCCCTCTCCTCCCTCACTTACCTCGACCTCTCCTTCAACAATCTCACCGGGTCCATCCCGAGCTCCCTCGCCAACCTCCAGCGCCTAGGCGCTCTCCACTTGGACCGCAACAAGTTAACCGGCCCCATCCCGCACTCCTTCGGATACTTCAAGGGAAAAATACCCTACCTTTACCTCTCCCACAACCAACTGTCAGGAAAAATACCAGCTTCCTTGGGGAGGGTGAATTTCAATGTGATTGATCTGTCTAGGAACAAACTCGAGGGCGATGCGTCGGTGATATTTGGCGGGAATAAAACGACCGAGATCATGGATTTGTCGAGGAACTTGCTGGAGTTTGATTTGTCTAAGGTTGTGTTTCCTCGGAGTGTGACTTGGTTGGATCTTAATCATAATAAGATATTCGGAGGGATTCCTGAGAAGGTGGTGGAGCTGGAGTTGCAGCTGCTGAATGTGAGTTATAATAGGCTGTGTGGTCGGATTCCGGTCGGGGGAAGGTTGCAGAGGTTTGATGTCTATGAGTACTTCCATAATAAGTGCTTGTGTGGGAAGCCACTCGGCAGCTGCAAATGATGTATTTGGAAAAGATGAACTCATTGCCAAATATCTTTTAGTATTTTTCCTCTGTGTTTACTTTTTTATGGTGGATCTGGCTTGTGGTTTTGTTTGAGTTGTTCTTTGAAGCTTGGATTTGCTAGATTTTGAGTTGGGGATTGGAAGGATGAGTTTAATGTGTAAATAATGAAGTATGAGTTTGTGTGCACTACTAGCATTGAAGTTTTTACTGACCAAAAA

mRNA sequence

ATGGCTGCTCCAAAGCTCACCTTCTTCTTCCTCCTCTTCATCTCCTCCTCCATTTGTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGTCCTCCTCAAAATCAAAAAATCCTTCAACAATCCCTACATCCTCACCTCATGGAAACCCGAAGAAGACTGCTGCAGTTGGTACTGTGTCCAATGTCATCCTACTTCTCACCGTATCATCTCCCTCACCATCTTCGCGGACGACAAACTCTCCGGCCAAATCCCCCCGGTGGTCGGCGATCTCCCGTTCCTCGAAACCCTGATGCTCCACAAGCTCCCCAATCTCACCGGCCCCATCCCCGCCGCCGTCGCCAATCTCCACAACCTCAAATCCCTCGACCTCAGCTGGAACGGCCTCTCCGGTCCGGTCCCGGAGTTCCTCGGCTCCCTCTCCTCCCTCACTTACCTCGACCTCTCCTTCAACAATCTCACCGGGTCCATCCCGAGCTCCCTCGCCAACCTCCAGCGCCTAGGCGCTCTCCACTTGGACCGCAACAAGTTAACCGGCCCCATCCCGCACTCCTTCGGATACTTCAAGGGAAAAATACCCTACCTTTACCTCTCCCACAACCAACTGTCAGGAAAAATACCAGCTTCCTTGGGGAGGGTGAATTTCAATGTGATTGATCTGTCTAGGAACAAACTCGAGGGCGATGCGTCGGTGATATTTGGCGGGAATAAAACGACCGAGATCATGGATTTGTCGAGGAACTTGCTGGAGTTTGATTTGTCTAAGGTTGTGTTTCCTCGGAGTGTGACTTGGTTGGATCTTAATCATAATAAGATATTCGGAGGGATTCCTGAGAAGGTGGTGGAGCTGGAGTTGCAGCTGCTGAATGTGAGTTATAATAGGCTGTGTGGTCGGATTCCGGTCGGGGGAAGGTTGCAGAGGTTTGATGTCTATGAGTACTTCCATAATAAGTGCTTGTGTGGGAAGCCACTCGGCAGCTGCAAATGA

Coding sequence (CDS)

ATGGCTGCTCCAAAGCTCACCTTCTTCTTCCTCCTCTTCATCTCCTCCTCCATTTGTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGTCCTCCTCAAAATCAAAAAATCCTTCAACAATCCCTACATCCTCACCTCATGGAAACCCGAAGAAGACTGCTGCAGTTGGTACTGTGTCCAATGTCATCCTACTTCTCACCGTATCATCTCCCTCACCATCTTCGCGGACGACAAACTCTCCGGCCAAATCCCCCCGGTGGTCGGCGATCTCCCGTTCCTCGAAACCCTGATGCTCCACAAGCTCCCCAATCTCACCGGCCCCATCCCCGCCGCCGTCGCCAATCTCCACAACCTCAAATCCCTCGACCTCAGCTGGAACGGCCTCTCCGGTCCGGTCCCGGAGTTCCTCGGCTCCCTCTCCTCCCTCACTTACCTCGACCTCTCCTTCAACAATCTCACCGGGTCCATCCCGAGCTCCCTCGCCAACCTCCAGCGCCTAGGCGCTCTCCACTTGGACCGCAACAAGTTAACCGGCCCCATCCCGCACTCCTTCGGATACTTCAAGGGAAAAATACCCTACCTTTACCTCTCCCACAACCAACTGTCAGGAAAAATACCAGCTTCCTTGGGGAGGGTGAATTTCAATGTGATTGATCTGTCTAGGAACAAACTCGAGGGCGATGCGTCGGTGATATTTGGCGGGAATAAAACGACCGAGATCATGGATTTGTCGAGGAACTTGCTGGAGTTTGATTTGTCTAAGGTTGTGTTTCCTCGGAGTGTGACTTGGTTGGATCTTAATCATAATAAGATATTCGGAGGGATTCCTGAGAAGGTGGTGGAGCTGGAGTTGCAGCTGCTGAATGTGAGTTATAATAGGCTGTGTGGTCGGATTCCGGTCGGGGGAAGGTTGCAGAGGTTTGATGTCTATGAGTACTTCCATAATAAGTGCTTGTGTGGGAAGCCACTCGGCAGCTGCAAATGA

Protein sequence

MAAPKLTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQRFDVYEYFHNKCLCGKPLGSCK

Homology

[Close]

BLAST of MC01g_new0209 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.2e-126
Identity = 219/325 (67.38%), Postives = 256/325 (78.77%), Query Frame = 0


Query: 7   TFFFLLFISSSI---CFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCH 66
           TF  L  + SS+     ++LCNP+DKKVLL+IKK+F +PY+L SWK + DCC WYCV C 
Sbjct: 7   TFLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCD 66

Query: 67  PTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLD 126
            T++RI SLTIFA  ++SGQIP +VGDLP+LETL  HK PNLTGPI  A+A L  LKSL 
Sbjct: 67  STTNRINSLTIFA-GQVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLR 126

Query: 127 LSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPH 186
           LSW  LSG VP+FL  L +LT+LDLSFNNLTG+IPSSL+ L  LGAL LDRNKLTG IP 
Sbjct: 127 LSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPI 186

Query: 187 SFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMD 246
           SFG F G +P LYLSHNQLSG IP S  +++F  IDLSRNKLEGDASVIFG NKTT+I+D
Sbjct: 187 SFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVD 246

Query: 247 LSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGG 306
           LSRNLLEF+LSKV FP S+T LD+NHNKI+G IP +  +L  Q LNVSYNRLCG+IPVGG
Sbjct: 247 LSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVGG 306

Query: 307 RLQRFDVYEYFHNKCLCGKPLGSCK 329
           +LQ FD Y YFHN+CLCG PL SCK
Sbjct: 307 KLQSFDEYSYFHNRCLCGAPLPSCK 330

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BLAST of MC01g_new0209 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.1e-120
Identity = 208/320 (65.00%), Postives = 245/320 (76.56%), Query Frame = 0


Query: 11  LLFISSSIC--FAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTSHR 70
           L+ +++  C   +E CNP DKKVLL+IKK+ +NPYIL SW P  DCC WYCV+C  T+HR
Sbjct: 15  LVLLATRPCPSLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTTHR 74

Query: 71  IISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWNG 130
           I SLTIF+  +LSGQIP  VGDLPFLETL+  KL NLTG IP A+A L +LK + LSW  
Sbjct: 75  INSLTIFS-GQLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTN 134

Query: 131 LSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGYF 190
           LSGPVP F   L +LTYLDLSFNNL+G IP SL+ L  LGALHLDRN LTGPIP SFG F
Sbjct: 135 LSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFGKF 194

Query: 191 KGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMDLSRNL 250
            G  P L+LSHNQLSGKIP S    + NV+DLSRNKLEGD S+ F  NK+T+I+D SRNL
Sbjct: 195 AGSTPGLHLSHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFSRNL 254

Query: 251 LEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQRF 310
            +FDLS+V FP+S+T LDL+HNKI G +PE +  L+LQ LNVSYNRLCG+IPVGG+LQ F
Sbjct: 255 FQFDLSRVEFPKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQSF 314

Query: 311 DVYEYFHNKCLCGKPLGSCK 329
           D   YFHN+CLCG PL SCK
Sbjct: 315 DYDSYFHNRCLCGAPLQSCK 333

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BLAST of MC01g_new0209 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.2e-111
Identity = 193/325 (59.38%), Postives = 237/325 (72.92%), Query Frame = 0


Query: 6   LTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQC--H 65
           L   FL    ++    +LCN NDK  LLKIKKS NNPY L SW P+ DCCSWYC++C   
Sbjct: 7   LCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDA 66

Query: 66  PTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLD 125
             +HR+ +LTIF+  ++SGQIP  VGDLP+LETL+  KL NLTG I   +A L NL+ L 
Sbjct: 67  TVNHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLR 126

Query: 126 LSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPH 185
           LSW  L+GP+P+F+  L +L +L+LSFN+L+GSIPSSL+ L ++ AL L RNKLTG IP 
Sbjct: 127 LSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPE 186

Query: 186 SFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMD 245
           SFG F G +P L LSHNQLSG IP SLG ++FN IDLSRNKL+GDAS++FG NKTT  +D
Sbjct: 187 SFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSID 246

Query: 246 LSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGG 305
           LSRN+ +FD+SKV  P+++  LDLNHN I G IP +  E  LQ  NVSYN+LCG IP GG
Sbjct: 247 LSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTGG 306

Query: 306 RLQRFDVYEYFHNKCLCGKPLGSCK 329
           +LQ FD Y YFHNKCLCG PL  CK
Sbjct: 307 KLQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of MC01g_new0209 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 399.8 bits (1026), Expect = 2.9e-110
Identity = 195/327 (59.63%), Postives = 240/327 (73.39%), Query Frame = 0


Query: 6   LTFFFLL--FISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQC- 65
           +T F LL   + ++    +LC+ +DK  LLKIKKS NNPY L SW P+ DCCSWYC++C 
Sbjct: 5   MTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECG 64

Query: 66  -HPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKS 125
               +HR+ SL I  D ++SGQIPP VGDLP+L +L+  KL NLTG I   +A L NL  
Sbjct: 65  DATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTF 124

Query: 126 LDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPI 185
           L LSW  L+GPVPEFL  L +L Y+DLSFN+L+GSIPSSL++L++L  L L RNKLTGPI
Sbjct: 125 LRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPI 184

Query: 186 PHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEI 245
           P SFG F GK+P L+LSHNQLSG IP SLG  +F  IDLSRNKL+GDAS++FG  KTT I
Sbjct: 185 PESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWI 244

Query: 246 MDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPV 305
           +D+SRN+ +FDLSKV   +++  LD+NHN I G IP +  +   QLLNVSYNRLCGRIP 
Sbjct: 245 VDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPK 304

Query: 306 GGRLQRFDVYEYFHNKCLCGKPLGSCK 329
           G  +QRFD Y +FHNKCLCG PL SCK
Sbjct: 305 GEYIQRFDSYSFFHNKCLCGAPLPSCK 330

[Close]

BLAST of MC01g_new0209 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.0e-86
Identity = 162/321 (50.47%), Postives = 213/321 (66.36%), Query Frame = 0


Query: 8   FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCS-WYCVQCHPTS 67
           FF +LFI+    ++  C  NDK  LL+IKK+  NP +L+SW P  DCC+ W  V+C  T+
Sbjct: 10  FFSILFITLPSSYS--CTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--TN 69

Query: 68  HRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSW 127
            R+  L++    ++SGQI   +GDL  L TL    LP+LTG IP  +  L NL +L L  
Sbjct: 70  RRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKH 129

Query: 128 NGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFG 187
             LSGP+P+++  L SLT+LDLSFN  TG IP SL+ + +L A+ ++ NKLTG IP+SFG
Sbjct: 130 TSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFG 189

Query: 188 YFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMDLSR 247
            F G +P LYLS+N+LSGKIP SL + +FN +DLS N  EGDA + FG NKTT  +DLSR
Sbjct: 190 SFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSR 249

Query: 248 NLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQ 307
           N+  FDL KV F RS+  LDL+ N I+G IP  + +L L+  NVS N LCG+IP GG LQ
Sbjct: 250 NMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLLQ 309

Query: 308 RFDVYEYFHNKCLCGKPLGSC 328
            F+   + HN CLCG PL +C
Sbjct: 310 TFEPSAFAHNICLCGTPLKAC 325

[Close]

BLAST of MC01g_new0209 vs. NCBI nr
Match: XP_022151650.1 (polygalacturonase inhibitor-like [Momordica charantia] >ARM53420.1 pgip1 [Momordica charantia])

HSP 1 Score: 679 bits (1753), Expect = 7.53e-247
Identity = 327/328 (99.70%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MAAPKLTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCV 60
           MAAPKLTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCV
Sbjct: 1   MAAPKLTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCV 60

Query: 61  QCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLK 120
           QCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLK
Sbjct: 61  QCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLK 120

Query: 121 SLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGP 180
           SLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGP
Sbjct: 121 SLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGP 180

Query: 181 IPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTE 240
           IPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTE
Sbjct: 181 IPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTE 240

Query: 241 IMDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIP 300
           I+DLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIP
Sbjct: 241 IVDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIP 300

Query: 301 VGGRLQRFDVYEYFHNKCLCGKPLGSCK 328
           VGGRLQRFDVYEYFHNKCLCGKPLGSCK
Sbjct: 301 VGGRLQRFDVYEYFHNKCLCGKPLGSCK 328

[Close]

BLAST of MC01g_new0209 vs. NCBI nr
Match: XP_022151649.1 (polygalacturonase inhibitor-like [Momordica charantia])

HSP 1 Score: 554 bits (1428), Expect = 3.20e-197
Identity = 264/334 (79.04%), Postives = 296/334 (88.62%), Query Frame = 0


Query: 1   MAAPKLT------FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDC 60
           MA P L       FFF  F+  +   +ELCNPNDKKVLLKIK+S NNPYILTSWKP+EDC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 61  CSWYCVQCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVA 120
           C+WYCVQCHPTSHRIISLTIFADD+LSGQIPP VGDLPFLETLMLHKLPNL+G +PAAVA
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 121 NLHNLKSLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDR 180
           +L NLK LDLS NGLSGPVPEFLGSL++LT+LDLSFNN TG++PSSLA+LQRLGALHLDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 181 NKLTGPIPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFG 240
           NKLTG IPHSFGYFKG+IP LYLSHNQLSG+IP SL  ++FNVIDLSRNKL+GDAS+IFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 241 GNKTTEIMDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNR 300
            NKTT+I+DLSRNLLEF+L+ VVFP+S+T LDLNHNKIFGGIP ++ +L LQ LNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

Query: 301 LCGRIPVGGRLQRFDVYEYFHNKCLCGKPLGSCK 328
           LCG IPVGG+LQ FDVYEYFHNKCLCG PLGSCK
Sbjct: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334

[Close]

BLAST of MC01g_new0209 vs. NCBI nr
Match: ARM53421.1 (pgip2 [Momordica charantia])

HSP 1 Score: 554 bits (1427), Expect = 4.54e-197
Identity = 264/334 (79.04%), Postives = 295/334 (88.32%), Query Frame = 0


Query: 1   MAAPKLT------FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDC 60
           MA P L       FFF  F+  +   +ELCNPNDKKVLLKIK+S NNPYILTSWKP+EDC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 61  CSWYCVQCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVA 120
           C+WYCVQCHPTSHRIISLTIFADD+LSGQIPP VGDLPFLETLMLHKLPNL+G +PAAVA
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 121 NLHNLKSLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDR 180
           +L NLK LDLS NGLSGPVPEFLGSL++LT+LDLSFNN TG++PSSLA+LQRLGALHLDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 181 NKLTGPIPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFG 240
           NKLTG IPHSFGYFKG+IP LYLSHNQLSG+IP SL  ++FNVIDLSRNKL+GDAS+IFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 241 GNKTTEIMDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNR 300
            NKTT+I+DLSRNLLEF L+ VVFP+S+T LDLNHNKIFGGIP ++ +L LQ LNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFSLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

Query: 301 LCGRIPVGGRLQRFDVYEYFHNKCLCGKPLGSCK 328
           LCG IPVGG+LQ FDVYEYFHNKCLCG PLGSCK
Sbjct: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334

[Close]

BLAST of MC01g_new0209 vs. NCBI nr
Match: NP_001303611.1 (polygalacturonase inhibitor-like precursor [Cucumis melo] >AAP41199.1 polygalacturonase-inhibiting protein [Cucumis melo] >KAA0053950.1 polygalacturonase inhibitor-like precursor [Cucumis melo var. makuwa] >TYK04261.1 polygalacturonase inhibitor-like precursor [Cucumis melo var. makuwa])

HSP 1 Score: 547 bits (1409), Expect = 1.87e-194
Identity = 255/318 (80.19%), Postives = 282/318 (88.68%), Query Frame = 0


Query: 11  LLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTSHRII 70
           LLF   ++ FAELC+PNDK+VLL IKK+FNNPYILTSWKPEEDCC+WYCV+C   SHRII
Sbjct: 9   LLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHRII 68

Query: 71  SLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWNGLS 130
           +LTIFADD+LSG IPP VGDLPFLE LM HKLPNLTGPIP  +A LHNLK LDLSWNGLS
Sbjct: 69  ALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNGLS 128

Query: 131 GPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGYFKG 190
           GP+P FLGSLS+L  LDLSFN  TGSIPSSLANL+RLG LHLDRNKLTGPIP SFG FKG
Sbjct: 129 GPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNFKG 188

Query: 191 KIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMDLSRNLLE 250
           K+PYLYLSHNQLSGKIP S+G+V+FN IDLSRNKL GD S+IFG  KTTEI+DLSRNLLE
Sbjct: 189 KVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNLLE 248

Query: 251 FDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQRFDV 310
           F++SKVVFPR++TWLDLNHNKIFG IP  VV+LELQ+LNVSYN LCGRIP+GG+LQ FDV
Sbjct: 249 FNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSFDV 308

Query: 311 YEYFHNKCLCGKPLGSCK 328
           Y YFHNKCLCGKPLGSCK
Sbjct: 309 YSYFHNKCLCGKPLGSCK 326

[Close]

BLAST of MC01g_new0209 vs. NCBI nr
Match: AFY10521.1 (polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis])

HSP 1 Score: 545 bits (1404), Expect = 1.08e-193
Identity = 254/318 (79.87%), Postives = 281/318 (88.36%), Query Frame = 0


Query: 11  LLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTSHRII 70
           LLF   ++ FAELC+PNDK+VLL IKK+FNNPYILTSWKPEEDCC+WYCV+C   SHRI 
Sbjct: 9   LLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHRIT 68

Query: 71  SLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWNGLS 130
           +LTIFADD+LSG IPP VGDLPFLE LM HKLPNLTGPIP  +A LHNLK LDLSWNGLS
Sbjct: 69  ALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNGLS 128

Query: 131 GPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGYFKG 190
           GP+P FLGSLS+L  LDLSFN  TGSIPSSLANL+RLG LHLDRNKLTGPIP SFG FKG
Sbjct: 129 GPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNFKG 188

Query: 191 KIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMDLSRNLLE 250
           K+PYLYLSHNQLSGKIP S+G+V+FN IDLSRNKL GD S+IFG  KTTEI+DLSRNLLE
Sbjct: 189 KVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNLLE 248

Query: 251 FDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQRFDV 310
           F++SKVVFPR++TWLDLNHNKIFG IP  VV+LELQ+LNVSYN LCGRIP+GG+LQ FDV
Sbjct: 249 FNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSFDV 308

Query: 311 YEYFHNKCLCGKPLGSCK 328
           Y YFHNKCLCGKPLGSCK
Sbjct: 309 YSYFHNKCLCGKPLGSCK 326

[Close]

BLAST of MC01g_new0209 vs. ExPASy TrEMBL
Match: A0A1W6IYG8 (Pgip1 OS=Momordica charantia OX=3673 GN=LOC111019559 PE=2 SV=1)

HSP 1 Score: 679 bits (1753), Expect = 3.65e-247
Identity = 327/328 (99.70%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MAAPKLTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCV 60
           MAAPKLTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCV
Sbjct: 1   MAAPKLTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCV 60

Query: 61  QCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLK 120
           QCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLK
Sbjct: 61  QCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLK 120

Query: 121 SLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGP 180
           SLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGP
Sbjct: 121 SLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGP 180

Query: 181 IPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTE 240
           IPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTE
Sbjct: 181 IPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTE 240

Query: 241 IMDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIP 300
           I+DLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIP
Sbjct: 241 IVDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIP 300

Query: 301 VGGRLQRFDVYEYFHNKCLCGKPLGSCK 328
           VGGRLQRFDVYEYFHNKCLCGKPLGSCK
Sbjct: 301 VGGRLQRFDVYEYFHNKCLCGKPLGSCK 328

[Close]

BLAST of MC01g_new0209 vs. ExPASy TrEMBL
Match: A0A6J1DDN9 (polygalacturonase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111019558 PE=4 SV=1)

HSP 1 Score: 554 bits (1428), Expect = 1.55e-197
Identity = 264/334 (79.04%), Postives = 296/334 (88.62%), Query Frame = 0


Query: 1   MAAPKLT------FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDC 60
           MA P L       FFF  F+  +   +ELCNPNDKKVLLKIK+S NNPYILTSWKP+EDC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 61  CSWYCVQCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVA 120
           C+WYCVQCHPTSHRIISLTIFADD+LSGQIPP VGDLPFLETLMLHKLPNL+G +PAAVA
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 121 NLHNLKSLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDR 180
           +L NLK LDLS NGLSGPVPEFLGSL++LT+LDLSFNN TG++PSSLA+LQRLGALHLDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 181 NKLTGPIPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFG 240
           NKLTG IPHSFGYFKG+IP LYLSHNQLSG+IP SL  ++FNVIDLSRNKL+GDAS+IFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 241 GNKTTEIMDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNR 300
            NKTT+I+DLSRNLLEF+L+ VVFP+S+T LDLNHNKIFGGIP ++ +L LQ LNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

Query: 301 LCGRIPVGGRLQRFDVYEYFHNKCLCGKPLGSCK 328
           LCG IPVGG+LQ FDVYEYFHNKCLCG PLGSCK
Sbjct: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334

[Close]

BLAST of MC01g_new0209 vs. ExPASy TrEMBL
Match: A0A1W6IYI7 (Pgip2 OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 554 bits (1427), Expect = 2.20e-197
Identity = 264/334 (79.04%), Postives = 295/334 (88.32%), Query Frame = 0


Query: 1   MAAPKLT------FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDC 60
           MA P L       FFF  F+  +   +ELCNPNDKKVLLKIK+S NNPYILTSWKP+EDC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 61  CSWYCVQCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVA 120
           C+WYCVQCHPTSHRIISLTIFADD+LSGQIPP VGDLPFLETLMLHKLPNL+G +PAAVA
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 121 NLHNLKSLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDR 180
           +L NLK LDLS NGLSGPVPEFLGSL++LT+LDLSFNN TG++PSSLA+LQRLGALHLDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 181 NKLTGPIPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFG 240
           NKLTG IPHSFGYFKG+IP LYLSHNQLSG+IP SL  ++FNVIDLSRNKL+GDAS+IFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 241 GNKTTEIMDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNR 300
            NKTT+I+DLSRNLLEF L+ VVFP+S+T LDLNHNKIFGGIP ++ +L LQ LNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFSLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

Query: 301 LCGRIPVGGRLQRFDVYEYFHNKCLCGKPLGSCK 328
           LCG IPVGG+LQ FDVYEYFHNKCLCG PLGSCK
Sbjct: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334

[Close]

BLAST of MC01g_new0209 vs. ExPASy TrEMBL
Match: Q7X9Q7 (Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=2 SV=1)

HSP 1 Score: 547 bits (1409), Expect = 9.04e-195
Identity = 255/318 (80.19%), Postives = 282/318 (88.68%), Query Frame = 0


Query: 11  LLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTSHRII 70
           LLF   ++ FAELC+PNDK+VLL IKK+FNNPYILTSWKPEEDCC+WYCV+C   SHRII
Sbjct: 9   LLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHRII 68

Query: 71  SLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWNGLS 130
           +LTIFADD+LSG IPP VGDLPFLE LM HKLPNLTGPIP  +A LHNLK LDLSWNGLS
Sbjct: 69  ALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNGLS 128

Query: 131 GPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGYFKG 190
           GP+P FLGSLS+L  LDLSFN  TGSIPSSLANL+RLG LHLDRNKLTGPIP SFG FKG
Sbjct: 129 GPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNFKG 188

Query: 191 KIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMDLSRNLLE 250
           K+PYLYLSHNQLSGKIP S+G+V+FN IDLSRNKL GD S+IFG  KTTEI+DLSRNLLE
Sbjct: 189 KVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNLLE 248

Query: 251 FDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQRFDV 310
           F++SKVVFPR++TWLDLNHNKIFG IP  VV+LELQ+LNVSYN LCGRIP+GG+LQ FDV
Sbjct: 249 FNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSFDV 308

Query: 311 YEYFHNKCLCGKPLGSCK 328
           Y YFHNKCLCGKPLGSCK
Sbjct: 309 YSYFHNKCLCGKPLGSCK 326

[Close]

BLAST of MC01g_new0209 vs. ExPASy TrEMBL
Match: A0A5D3BX47 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4559G00050 PE=4 SV=1)

HSP 1 Score: 547 bits (1409), Expect = 9.04e-195
Identity = 255/318 (80.19%), Postives = 282/318 (88.68%), Query Frame = 0


Query: 11  LLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTSHRII 70
           LLF   ++ FAELC+PNDK+VLL IKK+FNNPYILTSWKPEEDCC+WYCV+C   SHRII
Sbjct: 9   LLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHRII 68

Query: 71  SLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWNGLS 130
           +LTIFADD+LSG IPP VGDLPFLE LM HKLPNLTGPIP  +A LHNLK LDLSWNGLS
Sbjct: 69  ALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNGLS 128

Query: 131 GPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGYFKG 190
           GP+P FLGSLS+L  LDLSFN  TGSIPSSLANL+RLG LHLDRNKLTGPIP SFG FKG
Sbjct: 129 GPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNFKG 188

Query: 191 KIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMDLSRNLLE 250
           K+PYLYLSHNQLSGKIP S+G+V+FN IDLSRNKL GD S+IFG  KTTEI+DLSRNLLE
Sbjct: 189 KVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNLLE 248

Query: 251 FDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQRFDV 310
           F++SKVVFPR++TWLDLNHNKIFG IP  VV+LELQ+LNVSYN LCGRIP+GG+LQ FDV
Sbjct: 249 FNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSFDV 308

Query: 311 YEYFHNKCLCGKPLGSCK 328
           Y YFHNKCLCGKPLGSCK
Sbjct: 309 YSYFHNKCLCGKPLGSCK 326

[Close]

BLAST of MC01g_new0209 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 404.4 bits (1038), Expect = 8.4e-113
Identity = 193/325 (59.38%), Postives = 237/325 (72.92%), Query Frame = 0


Query: 6   LTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQC--H 65
           L   FL    ++    +LCN NDK  LLKIKKS NNPY L SW P+ DCCSWYC++C   
Sbjct: 7   LCLLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDA 66

Query: 66  PTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLD 125
             +HR+ +LTIF+  ++SGQIP  VGDLP+LETL+  KL NLTG I   +A L NL+ L 
Sbjct: 67  TVNHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLR 126

Query: 126 LSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPH 185
           LSW  L+GP+P+F+  L +L +L+LSFN+L+GSIPSSL+ L ++ AL L RNKLTG IP 
Sbjct: 127 LSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPE 186

Query: 186 SFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMD 245
           SFG F G +P L LSHNQLSG IP SLG ++FN IDLSRNKL+GDAS++FG NKTT  +D
Sbjct: 187 SFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSID 246

Query: 246 LSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGG 305
           LSRN+ +FD+SKV  P+++  LDLNHN I G IP +  E  LQ  NVSYN+LCG IP GG
Sbjct: 247 LSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTGG 306

Query: 306 RLQRFDVYEYFHNKCLCGKPLGSCK 329
           +LQ FD Y YFHNKCLCG PL  CK
Sbjct: 307 KLQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of MC01g_new0209 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 399.8 bits (1026), Expect = 2.1e-111
Identity = 195/327 (59.63%), Postives = 240/327 (73.39%), Query Frame = 0


Query: 6   LTFFFLL--FISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQC- 65
           +T F LL   + ++    +LC+ +DK  LLKIKKS NNPY L SW P+ DCCSWYC++C 
Sbjct: 5   MTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECG 64

Query: 66  -HPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKS 125
               +HR+ SL I  D ++SGQIPP VGDLP+L +L+  KL NLTG I   +A L NL  
Sbjct: 65  DATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTF 124

Query: 126 LDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPI 185
           L LSW  L+GPVPEFL  L +L Y+DLSFN+L+GSIPSSL++L++L  L L RNKLTGPI
Sbjct: 125 LRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPI 184

Query: 186 PHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEI 245
           P SFG F GK+P L+LSHNQLSG IP SLG  +F  IDLSRNKL+GDAS++FG  KTT I
Sbjct: 185 PESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWI 244

Query: 246 MDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPV 305
           +D+SRN+ +FDLSKV   +++  LD+NHN I G IP +  +   QLLNVSYNRLCGRIP 
Sbjct: 245 VDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPK 304

Query: 306 GGRLQRFDVYEYFHNKCLCGKPLGSCK 329
           G  +QRFD Y +FHNKCLCG PL SCK
Sbjct: 305 GEYIQRFDSYSFFHNKCLCGAPLPSCK 330

[Close]

BLAST of MC01g_new0209 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 321.6 bits (823), Expect = 7.2e-88
Identity = 162/321 (50.47%), Postives = 213/321 (66.36%), Query Frame = 0


Query: 8   FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCS-WYCVQCHPTS 67
           FF +LFI+    ++  C  NDK  LL+IKK+  NP +L+SW P  DCC+ W  V+C  T+
Sbjct: 10  FFSILFITLPSSYS--CTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--TN 69

Query: 68  HRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSW 127
            R+  L++    ++SGQI   +GDL  L TL    LP+LTG IP  +  L NL +L L  
Sbjct: 70  RRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKH 129

Query: 128 NGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFG 187
             LSGP+P+++  L SLT+LDLSFN  TG IP SL+ + +L A+ ++ NKLTG IP+SFG
Sbjct: 130 TSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFG 189

Query: 188 YFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIMDLSR 247
            F G +P LYLS+N+LSGKIP SL + +FN +DLS N  EGDA + FG NKTT  +DLSR
Sbjct: 190 SFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSR 249

Query: 248 NLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNRLCGRIPVGGRLQ 307
           N+  FDL KV F RS+  LDL+ N I+G IP  + +L L+  NVS N LCG+IP GG LQ
Sbjct: 250 NMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLLQ 309

Query: 308 RFDVYEYFHNKCLCGKPLGSC 328
            F+   + HN CLCG PL +C
Sbjct: 310 TFEPSAFAHNICLCGTPLKAC 325

[Close]

BLAST of MC01g_new0209 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.3e-48
Identity = 119/366 (32.51%), Postives = 185/366 (50.55%), Query Frame = 0


Query: 1   MAAPKLTFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPY--ILTSWKPEEDCC-SW 60
           +A+P  +   ++FI S I     C+P D+  L   K S + P   I  +W    DCC  W
Sbjct: 5   LASPFSSLLAVVFI-SVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEW 64

Query: 61  YCVQCHPTSHRIISLTIFADDK------------LSGQIPPVVGDLPFLETLMLHKLPNL 120
           Y + C P S R+  +++  + +            +SG I P V DL  L +L+L     +
Sbjct: 65  YGISCDPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGI 124

Query: 121 TGPIPAAVANLHNLKSLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQ 180
           TG IP  + +L +L+ LDL+ N ++G +P  +G LS L  L+L+ N ++G IP+SL +L 
Sbjct: 125 TGEIPPCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLI 184

Query: 181 RLGALHLDRNKLTGPIPHSFGYFKGKIPYLYLSHNQLSGKIPASL-GRVNFNVIDLSRNK 240
            L  L L  N +TG IP  FG  K  +  + L  N+L+G IP S+ G      +DLS+N 
Sbjct: 185 ELKHLELTENGITGVIPADFGSLK-MLSRVLLGRNELTGSIPESISGMERLADLDLSKNH 244

Query: 241 LEG-----------------DASVIFG-------GNKTTEIMDLSRNLLEFDLSKVVFPR 300
           +EG                 D + + G        N   ++ +LSRN LE  +  V   +
Sbjct: 245 IEGPIPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSK 304

Query: 301 S-VTWLDLNHNKIFGGIPEKVVELE-LQLLNVSYNRLCGRIPVGGRLQRFDVYEYFHNKC 325
           + +  LDL+HN + G IP+ +   + +  L++S+N+LCGRIP G      +   +  N+C
Sbjct: 305 TYLVSLDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQC 364

[Close]

BLAST of MC01g_new0209 vs. TAIR 10
Match: AT5G12940.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 114/345 (33.04%), Postives = 171/345 (49.57%), Query Frame = 0


Query: 24  CNPNDKKVLLKIKKSFNNPYI--LTSWKPEEDCCSWYCVQCHPTSHRIISLTIFADDK-- 83
           C P+D+  LL+ +   N PYI    +WK  + C  WY V C P + R+  +T+  + +  
Sbjct: 27  CLPSDRAALLEFRAKLNEPYIGVFNTWKGLDCCKGWYGVSCDPNTRRVAGITLRGESEDP 86

Query: 84  ----------LSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSWNGL 143
                     ++G I P +  L  L  +++     ++G IP+ + NL  L+ LDL  N  
Sbjct: 87  LFQKAKRSGLMTGSISPSICKLTRLSGIIIADWKGISGVIPSCIENLPFLRHLDLVGNKF 146

Query: 144 SGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFGYFK 203
           SG +P  +G L  L  L+L+ N+L G IP S+  L  L  L L  N ++G IP   G  K
Sbjct: 147 SGVIPANIGKLLRLKVLNLADNHLYGVIPPSITRLVSLSHLDLRNNNISGVIPRDIGRLK 206

Query: 204 GKIPYLYLSHNQLSGKIPASLGRV-NFNVIDLSRNKLEGDASVIFG-----------GN- 263
             +  + LS N++SG+IP SL R+     ++LS N+L G     FG           GN 
Sbjct: 207 -MVSRVLLSGNKISGQIPDSLTRIYRLADLELSMNRLTGPIPASFGKMSVLATLNLDGNL 266

Query: 264 -----------KTTEIMDLSRNLLEFDLSKVVFPRS-VTWLDLNHNKIFGGIPEKVVELE 323
                       +   ++LS NL+   +     PRS  T LDL +N++ G IP  +    
Sbjct: 267 ISGMIPGSLLASSISNLNLSGNLITGSIPNTFGPRSYFTVLDLANNRLQGPIPASITAAS 326

Query: 324 -LQLLNVSYNRLCGRIPVGGRLQRFDVYEYFHNKCLCGKPLGSCK 329
            +  L+VS+N LCG+IP+G      D   + +N CLCGKPLG+CK
Sbjct: 327 FIGHLDVSHNHLCGKIPMGSPFDHLDATSFAYNACLCGKPLGNCK 370

The following BLAST results are available for this feature:

BLAST of MC01g_new0209 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05091	2.2e-126	67.38	Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1	[more]
A7PW81	1.1e-120	65.00	Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1	[more]
Q9M5J9	1.2e-111	59.38	Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1	[more]
Q9M5J8	2.9e-110	59.63	Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2	[more]
Q9LH52	1.0e-86	50.47	Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ...	[more]

BLAST of MC01g_new0209 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151650.1	7.53e-247	99.70	polygalacturonase inhibitor-like [Momordica charantia] >ARM53420.1 pgip1 [Momord...	[more]
XP_022151649.1	3.20e-197	79.04	polygalacturonase inhibitor-like [Momordica charantia]	[more]
ARM53421.1	4.54e-197	79.04	pgip2 [Momordica charantia]	[more]
NP_001303611.1	1.87e-194	80.19	polygalacturonase inhibitor-like precursor [Cucumis melo] >AAP41199.1 polygalact...	[more]
AFY10521.1	1.08e-193	79.87	polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis]	[more]

BLAST of MC01g_new0209 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1W6IYG8	3.65e-247	99.70	Pgip1 OS=Momordica charantia OX=3673 GN=LOC111019559 PE=2 SV=1	[more]
A0A6J1DDN9	1.55e-197	79.04	polygalacturonase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111019558 ...	[more]
A0A1W6IYI7	2.20e-197	79.04	Pgip2 OS=Momordica charantia OX=3673 PE=2 SV=1	[more]
Q7X9Q7	9.04e-195	80.19	Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=...	[more]
A0A5D3BX47	9.04e-195	80.19	Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of MC01g_new0209 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06860.1	8.4e-113	59.38	polygalacturonase inhibiting protein 1	[more]
AT5G06870.1	2.1e-111	59.63	polygalacturonase inhibiting protein 2	[more]
AT3G12145.1	7.2e-88	50.47	Leucine-rich repeat (LRR) family protein	[more]
AT3G12610.1	3.3e-48	32.51	Leucine-rich repeat (LRR) family protein	[more]
AT5G12940.1	2.8e-47	33.04	Leucine-rich repeat (LRR) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00019	LEURICHRPT	coord: 140..153 score: 54.6 coord: 119..132 score: 54.04
None	No IPR available	PANTHER	PTHR48059:SF10	POLYGALACTURONASE INHIBITOR 2	coord: 10..327
None	No IPR available	PANTHER	PTHR48059	POLYGALACTURONASE INHIBITOR 1	coord: 10..327
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 23..326
IPR013210	Leucine-rich repeat-containing N-terminal, plant-type	PFAM	PF08263	LRRNT_2	coord: 26..62 e-value: 1.5E-10 score: 41.2
IPR001611	Leucine-rich repeat	PFAM	PF13855	LRR_8	coord: 118..177 e-value: 6.7E-8 score: 32.2
IPR001611	Leucine-rich repeat	PROSITE	PS51450	LRR	coord: 142..165 score: 7.419328
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 22..328 e-value: 5.4E-86 score: 290.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g_new0209.1	MC01g_new0209.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0090353	polygalacturonase inhibitor activity
molecular_function	GO:0005515	protein binding