MC01g_new0205 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0205
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	E3 ubiquitin-protein ligase RNF181-like
Location	MC01: 7537117 .. 7537806 (+)
RNA-Seq Expression	MC01g_new0205
Synteny	MC01g_new0205

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTTTCGCTTCTTCTTCTTCTTCTTCTTCTTCATTGCTCCGCTACTTTCTCCACGGCACCGTCGGCCCCTCCTCCGGCGACCCTCCGTTTCAATTTCTCGAAGTCCGATTGGGGATGATCTCCACCGAAGCTCCCTTCAGTGCTCCAATGGCGATGGCCAACGCCGTGTTCCCTCTCTCTCTCCGCCGATTCTTCCATCACCGCCCTCCCTTCCACTACCTGCTCTGGATTCTGTCCTCCCTCGACCTCCCCCCCGCCGCCTCCCACGCCGTCGCTCGGAACATCGCCGCCTTCGTGACCCACTTGACCCGCGGGAACGCCGAGGTCGACTTACGCGTCGTCGCCGTCGTCGATTACATTCAGATCGCGGTTTCGGTGGGGCAGAGGCAGAGGCAGAGTCGGAGTTGGAACGCCGAGGAGAGTATGATTCTCGATGAAGTTCCGGCGCCGGAGCCGGCGCCGGAGTTTGTAGAAGAAGAAGAAGGAGGAGAATTGGGAGATTGCAGCATTTGTTTTGAGGAACTAAAAATTGACGGCCATGGCGGAAACGGGAGCGGGACGAAGAAGAATAAGGTGGTTAAGATTGGGTGTGGTCATGTGTACCACAAATCCTGCATCATCACTTGGCTTACTAACAAAAATTCATGCCCATTGTGTAGAATGCCCATTACTTTGAAGTTTTGA

mRNA sequence

ATGGCTTCTTTCGCTTCTTCTTCTTCTTCTTCTTCTTCATTGCTCCGCTACTTTCTCCACGGCACCGTCGGCCCCTCCTCCGGCGACCCTCCGTTTCAATTTCTCGAAGTCCGATTGGGGATGATCTCCACCGAAGCTCCCTTCAGTGCTCCAATGGCGATGGCCAACGCCGTGTTCCCTCTCTCTCTCCGCCGATTCTTCCATCACCGCCCTCCCTTCCACTACCTGCTCTGGATTCTGTCCTCCCTCGACCTCCCCCCCGCCGCCTCCCACGCCGTCGCTCGGAACATCGCCGCCTTCGTGACCCACTTGACCCGCGGGAACGCCGAGGTCGACTTACGCGTCGTCGCCGTCGTCGATTACATTCAGATCGCGGTTTCGGTGGGGCAGAGGCAGAGGCAGAGTCGGAGTTGGAACGCCGAGGAGAGTATGATTCTCGATGAAGTTCCGGCGCCGGAGCCGGCGCCGGAGTTTGTAGAAGAAGAAGAAGGAGGAGAATTGGGAGATTGCAGCATTTGTTTTGAGGAACTAAAAATTGACGGCCATGGCGGAAACGGGAGCGGGACGAAGAAGAATAAGGTGGTTAAGATTGGGTGTGGTCATGTGTACCACAAATCCTGCATCATCACTTGGCTTACTAACAAAAATTCATGCCCATTGTGTAGAATGCCCATTACTTTGAAGTTTTGA

Coding sequence (CDS)

ATGGCTTCTTTCGCTTCTTCTTCTTCTTCTTCTTCTTCATTGCTCCGCTACTTTCTCCACGGCACCGTCGGCCCCTCCTCCGGCGACCCTCCGTTTCAATTTCTCGAAGTCCGATTGGGGATGATCTCCACCGAAGCTCCCTTCAGTGCTCCAATGGCGATGGCCAACGCCGTGTTCCCTCTCTCTCTCCGCCGATTCTTCCATCACCGCCCTCCCTTCCACTACCTGCTCTGGATTCTGTCCTCCCTCGACCTCCCCCCCGCCGCCTCCCACGCCGTCGCTCGGAACATCGCCGCCTTCGTGACCCACTTGACCCGCGGGAACGCCGAGGTCGACTTACGCGTCGTCGCCGTCGTCGATTACATTCAGATCGCGGTTTCGGTGGGGCAGAGGCAGAGGCAGAGTCGGAGTTGGAACGCCGAGGAGAGTATGATTCTCGATGAAGTTCCGGCGCCGGAGCCGGCGCCGGAGTTTGTAGAAGAAGAAGAAGGAGGAGAATTGGGAGATTGCAGCATTTGTTTTGAGGAACTAAAAATTGACGGCCATGGCGGAAACGGGAGCGGGACGAAGAAGAATAAGGTGGTTAAGATTGGGTGTGGTCATGTGTACCACAAATCCTGCATCATCACTTGGCTTACTAACAAAAATTCATGCCCATTGTGTAGAATGCCCATTACTTTGAAGTTTTGA

Protein sequence

MASFASSSSSSSSLLRYFLHGTVGPSSGDPPFQFLEVRLGMISTEAPFSAPMAMANAVFPLSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVDYIQIAVSVGQRQRQSRSWNAEESMILDEVPAPEPAPEFVEEEEGGELGDCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLKF

Homology

[Close]

BLAST of MC01g_new0205 vs. ExPASy Swiss-Prot
Match: P90990 (E3 ubiquitin-protein ligase trul-1 OS=Caenorhabditis elegans OX=6239 GN=trul-1 PE=3 SV=3)

HSP 1 Score: 58.2 bits (139), Expect = 1.5e-07
Identity = 24/58 (41.38%), Postives = 31/58 (53.45%), Query Frame = 0


Query: 168 GDCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPI 226
           G CSICFE+LK           + +K+  I CGH+YH  CI  W+  K  CP CR  +
Sbjct: 11  GSCSICFEDLK-----------QNDKISAIVCGHIYHHGCISQWIATKRQCPSCRRTV 57

[Close]

BLAST of MC01g_new0205 vs. ExPASy Swiss-Prot
Match: Q9VE61 (E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN=CG7694 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 6.1e-06
Identity = 34/110 (30.91%), Postives = 55/110 (50.00%), Query Frame = 0


Query: 113 LRVVAVVDYIQIAVSVGQRQRQSRSWNAEESMILDEVPAPEPAPEFVEEEEGGELGDCSI 172
           L+V+A+++ I + + V +         A +  IL+      P  E V+ +EGG+L +CS+
Sbjct: 28  LQVLAIMNGIDMEIEVPE---------ASKRAILE-----LPVHEIVKSDEGGDL-ECSV 87

Query: 173 CFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCR 223
           C E  +           +  K   + C H +H+ CI+ WL   NSCPLCR
Sbjct: 88  CKEPAE-----------EGQKYRILPCKHEFHEECILLWLKKTNSCPLCR 111

[Close]

BLAST of MC01g_new0205 vs. ExPASy Swiss-Prot
Match: Q08109 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HRD1 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.0e-05
Identity = 20/56 (35.71%), Postives = 30/56 (53.57%), Query Frame = 0


Query: 170 CSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPI 226
           C IC +EL I          K  K  ++ CGH+ H SC+  W+    +CP+CR+P+
Sbjct: 349 CIICMDEL-IHSPNQQTWKNKNKKPKRLPCGHILHLSCLKNWMERSQTCPICRLPV 403

[Close]

BLAST of MC01g_new0205 vs. ExPASy Swiss-Prot
Match: Q9ULK6 (RING finger protein 150 OS=Homo sapiens OX=9606 GN=RNF150 PE=1 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 3.0e-05
Identity = 23/67 (34.33%), Postives = 37/67 (55.22%), Query Frame = 0


Query: 160 EEEEGGELGDCSICFEELKIDGHGGNGSGTKKNKVVKI-GCGHVYHKSCIITWLTNKNSC 219
           ++E   +  +C++C E            G K N VV+I  C H++HKSC+  WL +  +C
Sbjct: 268 DKETESDFDNCAVCIE------------GYKPNDVVRILPCRHLFHKSCVDPWLLDHRTC 322

Query: 220 PLCRMPI 226
           P+C+M I
Sbjct: 328 PMCKMNI 322

[Close]

BLAST of MC01g_new0205 vs. ExPASy Swiss-Prot
Match: Q5DTZ6 (RING finger protein 150 OS=Mus musculus OX=10090 GN=Rnf150 PE=2 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 3.0e-05
Identity = 23/67 (34.33%), Postives = 37/67 (55.22%), Query Frame = 0


Query: 160 EEEEGGELGDCSICFEELKIDGHGGNGSGTKKNKVVKI-GCGHVYHKSCIITWLTNKNSC 219
           ++E   +  +C++C E            G K N VV+I  C H++HKSC+  WL +  +C
Sbjct: 267 DKETESDFDNCAVCIE------------GYKPNDVVRILPCRHLFHKSCVDPWLLDHRTC 321

Query: 220 PLCRMPI 226
           P+C+M I
Sbjct: 327 PMCKMNI 321

[Close]

BLAST of MC01g_new0205 vs. NCBI nr
Match: XP_022151639.1 (uncharacterized protein LOC111019546 [Momordica charantia])

HSP 1 Score: 457 bits (1175), Expect = 4.62e-162
Identity = 227/229 (99.13%), Postives = 228/229 (99.56%), Query Frame = 0


Query: 1   MASFASSSSSSSSLLRYFLHGTVGPSSGDPPFQFLEVRLGMISTEAPFSAPMAMANAVFP 60
           MASFASSSSSSSSLLRYFLHGTVGPSSGDPPFQFLEVRLGMISTEAPFSAPMAMANAVFP
Sbjct: 1   MASFASSSSSSSSLLRYFLHGTVGPSSGDPPFQFLEVRLGMISTEAPFSAPMAMANAVFP 60

Query: 61  LSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVD 120
           LSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVD
Sbjct: 61  LSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVD 120

Query: 121 YIQIAVSVGQRQRQSRSWNAEESMILDEVPAPEPAPEFVEEEEGGELGDCSICFEELKID 180
           YIQIAVSVGQRQRQSRSWNA+ESMILDEVPAPEPAPEFVEEE GGELGDCSICFEELKID
Sbjct: 121 YIQIAVSVGQRQRQSRSWNADESMILDEVPAPEPAPEFVEEEGGGELGDCSICFEELKID 180

Query: 181 GHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLKF 229
           GHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLKF
Sbjct: 181 GHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLKF 229

[Close]

BLAST of MC01g_new0205 vs. NCBI nr
Match: KAG6595648.1 (hypothetical protein SDJN03_12201, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 134 bits (337), Expect = 7.30e-35
Identity = 81/229 (35.37%), Postives = 125/229 (54.59%), Query Frame = 0


Query: 15  LRYFLHGTVGPSSGDPPFQFLEVRLGMISTE---APFS--------APMAMANAVFPLSL 74
           ++Y ++G    +SG+ P+Q L+++L     E   +P S        AP+ + N +FPL L
Sbjct: 9   IQYRIYGYTSAASGEQPYQVLQLQLRYFRREISVSPLSGVTYLQETAPILLRNLLFPLRL 68

Query: 75  RRFFHHRPPFH--YLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVDY 134
           +    H P F   Y+  +LS L++       +A  I ++   +  GN E     V  +DY
Sbjct: 69  QEL--HDPVFLQVYVAQLLSLLNVRSPVREVIAERIVSYTLLVAGGNRETSFHTVLELDY 128

Query: 135 IQIAVSVGQRQRQSRSWN-----AEESMILDEVPAPEPAPEFVEEEEGGELGDCSICFEE 194
           I++   + +     + W+      EE+ IL+E     P  E VEE  G E+GDCSIC EE
Sbjct: 129 IRVITWMEENHGLFQGWSNDNAAGEETAILEEA---RPVTEKVEEN-GEEMGDCSICLEE 188

Query: 195 LKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPI 225
           L  +G+G NG G K+ +V+++ CGHVYH+SCI  WL + +SCPLCR P+
Sbjct: 189 LNGNGNG-NGEGKKREEVIRMQCGHVYHESCIFNWLHHNSSCPLCRAPL 230

[Close]

BLAST of MC01g_new0205 vs. NCBI nr
Match: XP_023518048.1 (uncharacterized protein LOC111781594 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 131 bits (330), Expect = 7.67e-34
Identity = 80/229 (34.93%), Postives = 126/229 (55.02%), Query Frame = 0


Query: 15  LRYFLHGTVGPSSGDPPFQFLEVRLGMISTE---APFS--------APMAMANAVFPLSL 74
           ++Y ++G    +SG+ P+Q L+++L  I  +   +P S        AP+ + N +FPL L
Sbjct: 9   IQYRIYGYTSAASGEQPYQVLQLQLHYIRRDISVSPLSGVTYLQETAPILLRNLLFPLPL 68

Query: 75  RRFFHHRPPFH--YLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVDY 134
           +    H P     Y+  +LS L++   A  A+A  I ++   +  GN E     V  +D+
Sbjct: 69  QEL--HDPVILQVYVAQLLSLLNVRSPACEAIAERIVSYTILMAGGNRETSFHTVVDLDF 128

Query: 135 IQIAVSVGQRQRQSRSWN-----AEESMILDEVPAPEPAPEFVEEEEGGELGDCSICFEE 194
           I++   + +     + W+      EE++IL+E     P  E VEE  G E+GDCSIC EE
Sbjct: 129 IRLITWMEENHGLFQGWSNGSAEGEETVILEEA---RPVTEKVEEN-GEEMGDCSICLEE 188

Query: 195 LKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPI 225
           L  +G   NG G K+ +V+++ CGHVYH+SCI  WL + +SCPLCR P+
Sbjct: 189 LNGNG---NGEGKKREEVIRMQCGHVYHESCIFNWLHHNSSCPLCRAPL 228

[Close]

BLAST of MC01g_new0205 vs. NCBI nr
Match: KAG6595650.1 (hypothetical protein SDJN03_12203, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 131 bits (330), Expect = 8.09e-34
Identity = 80/229 (34.93%), Postives = 124/229 (54.15%), Query Frame = 0


Query: 15  LRYFLHGTVGPSSGDPPFQFLEVRLGMISTE---APFS--------APMAMANAVFPLSL 74
           ++Y ++G    +SG+ P+Q L+++L     E   +P S        AP+ + N +FPL L
Sbjct: 9   IQYRIYGYTSAASGEQPYQVLQLQLRYFRREISVSPLSGVTYLQETAPILLRNLLFPLRL 68

Query: 75  RRFFHHRPPFH--YLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVDY 134
           +    H P F   Y+  +LS L++       +A  I ++   +  GN E     V  +DY
Sbjct: 69  QEL--HDPVFLQVYVAQLLSLLNVRSPVREVIAERIVSYTLLVAGGNRETSFHTVLELDY 128

Query: 135 IQIAVSVGQRQRQSRSWN-----AEESMILDEVPAPEPAPEFVEEEEGGELGDCSICFEE 194
           I++   + +     + W+      EE+ IL+E     P  E VEE  G E+GDCSIC EE
Sbjct: 129 IRLITWMEENHGLFQGWSNDNAAGEETAILEEA---RPVTEKVEEN-GEEMGDCSICLEE 188

Query: 195 LKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPI 225
           L  +G+G  G G K+ +V+++ CGHVYH+SCI  WL + +SCPLCR P+
Sbjct: 189 LNGNGNG-KGEGKKREEVIRMQCGHVYHESCIFNWLHHNSSCPLCRAPL 230

[Close]

BLAST of MC01g_new0205 vs. NCBI nr
Match: XP_022151640.1 (E3 ubiquitin-protein ligase RNF181-like [Momordica charantia])

HSP 1 Score: 125 bits (315), Expect = 7.80e-32
Identity = 93/226 (41.15%), Postives = 121/226 (53.54%), Query Frame = 0


Query: 8   SSSSSSLLRYFLHGTVGP-SSGDPPFQFLEVRLGMISTEAPFSAPMA---------MANA 67
           SSS+S  +RY LHG     SSGD P Q L+VRL   S     S P A         MANA
Sbjct: 2   SSSTSFSVRYCLHGHRSVVSSGDQPHQVLQVRLRSQSRMISRS-PAAILQETSWTPMANA 61

Query: 68  VFPLSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAE-VDLRVV 127
           VFPL L           Y+  +L SLDLP  A   +AR IA+FV  L  GN++ V+L VV
Sbjct: 62  VFPLPLSALNGLLFLEAYIRQLLCSLDLPEGACEVIARKIASFVVELAGGNSDQVNLHVV 121

Query: 128 AVVDYIQIAVSVGQRQRQSRSWNAEESMILDEVPAPEPAPEFVEEEEGGELGDCSICFEE 187
           A+VDYI+    V +      + +A   +  ++V            EE  ELGDCS+C  E
Sbjct: 122 ALVDYIEEMAIVAEEAPAGAAKSAISRLKKEKV------------EEEVELGDCSVCLNE 181

Query: 188 LKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCR 222
           +    +GG+       +V+K+ CGHVYH+SCI++WL   NSCPLCR
Sbjct: 182 M----NGGS-------EVIKMPCGHVYHESCILSWLNKNNSCPLCR 203

[Close]

BLAST of MC01g_new0205 vs. ExPASy TrEMBL
Match: A0A6J1DDM9 (uncharacterized protein LOC111019546 OS=Momordica charantia OX=3673 GN=LOC111019546 PE=4 SV=1)

HSP 1 Score: 457 bits (1175), Expect = 2.23e-162
Identity = 227/229 (99.13%), Postives = 228/229 (99.56%), Query Frame = 0


Query: 1   MASFASSSSSSSSLLRYFLHGTVGPSSGDPPFQFLEVRLGMISTEAPFSAPMAMANAVFP 60
           MASFASSSSSSSSLLRYFLHGTVGPSSGDPPFQFLEVRLGMISTEAPFSAPMAMANAVFP
Sbjct: 1   MASFASSSSSSSSLLRYFLHGTVGPSSGDPPFQFLEVRLGMISTEAPFSAPMAMANAVFP 60

Query: 61  LSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVD 120
           LSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVD
Sbjct: 61  LSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRVVAVVD 120

Query: 121 YIQIAVSVGQRQRQSRSWNAEESMILDEVPAPEPAPEFVEEEEGGELGDCSICFEELKID 180
           YIQIAVSVGQRQRQSRSWNA+ESMILDEVPAPEPAPEFVEEE GGELGDCSICFEELKID
Sbjct: 121 YIQIAVSVGQRQRQSRSWNADESMILDEVPAPEPAPEFVEEEGGGELGDCSICFEELKID 180

Query: 181 GHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLKF 229
           GHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLKF
Sbjct: 181 GHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLKF 229

[Close]

BLAST of MC01g_new0205 vs. ExPASy TrEMBL
Match: A0A6J1DBR5 (E3 ubiquitin-protein ligase RNF181-like OS=Momordica charantia OX=3673 GN=LOC111019547 PE=4 SV=1)

HSP 1 Score: 125 bits (315), Expect = 3.78e-32
Identity = 93/226 (41.15%), Postives = 121/226 (53.54%), Query Frame = 0


Query: 8   SSSSSSLLRYFLHGTVGP-SSGDPPFQFLEVRLGMISTEAPFSAPMA---------MANA 67
           SSS+S  +RY LHG     SSGD P Q L+VRL   S     S P A         MANA
Sbjct: 2   SSSTSFSVRYCLHGHRSVVSSGDQPHQVLQVRLRSQSRMISRS-PAAILQETSWTPMANA 61

Query: 68  VFPLSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAE-VDLRVV 127
           VFPL L           Y+  +L SLDLP  A   +AR IA+FV  L  GN++ V+L VV
Sbjct: 62  VFPLPLSALNGLLFLEAYIRQLLCSLDLPEGACEVIARKIASFVVELAGGNSDQVNLHVV 121

Query: 128 AVVDYIQIAVSVGQRQRQSRSWNAEESMILDEVPAPEPAPEFVEEEEGGELGDCSICFEE 187
           A+VDYI+    V +      + +A   +  ++V            EE  ELGDCS+C  E
Sbjct: 122 ALVDYIEEMAIVAEEAPAGAAKSAISRLKKEKV------------EEEVELGDCSVCLNE 181

Query: 188 LKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCR 222
           +    +GG+       +V+K+ CGHVYH+SCI++WL   NSCPLCR
Sbjct: 182 M----NGGS-------EVIKMPCGHVYHESCILSWLNKNNSCPLCR 203

[Close]

BLAST of MC01g_new0205 vs. ExPASy TrEMBL
Match: A0A5D3E1S5 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G00800 PE=4 SV=1)

HSP 1 Score: 119 bits (298), Expect = 2.26e-29
Identity = 68/180 (37.78%), Postives = 101/180 (56.11%), Query Frame = 0


Query: 56  NAVFPLSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRV 115
           NA+  L+LR+    + P  Y+   LSSL++ PAAS ++AR IA+ +  +   N + +  +
Sbjct: 58  NALVYLTLRQL---QSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHI 117

Query: 116 VAVVDYIQIAVSVGQRQRQSRSWNAEESMILDEVP-------APEPAPEFVEEEEGGELG 175
           +A VD+I++     + + QS    AEE+++ DE P                 EE+  +LG
Sbjct: 118 IAEVDFIRVIWL--ELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLG 177

Query: 176 DCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLK 228
           DCSIC +EL  +          K +V++I CGHVYH+SCI  WL N NSCPLCR P+ LK
Sbjct: 178 DCSICLDELSCE----------KREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLK 222

[Close]

BLAST of MC01g_new0205 vs. ExPASy TrEMBL
Match: A0A1S4DUZ4 (E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 PE=4 SV=1)

HSP 1 Score: 119 bits (298), Expect = 2.26e-29
Identity = 68/180 (37.78%), Postives = 101/180 (56.11%), Query Frame = 0


Query: 56  NAVFPLSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRV 115
           NA+  L+LR+    + P  Y+   LSSL++ PAAS ++AR IA+ +  +   N + +  +
Sbjct: 58  NALVYLTLRQL---QSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNFHI 117

Query: 116 VAVVDYIQIAVSVGQRQRQSRSWNAEESMILDEVP-------APEPAPEFVEEEEGGELG 175
           +A VD+I++     + + QS    AEE+++ DE P                 EE+  +LG
Sbjct: 118 IAEVDFIRVIWL--ELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLG 177

Query: 176 DCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITLK 228
           DCSIC +EL  +          K +V++I CGHVYH+SCI  WL N NSCPLCR P+ LK
Sbjct: 178 DCSICLDELSCE----------KREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPLK 222

[Close]

BLAST of MC01g_new0205 vs. ExPASy TrEMBL
Match: A0A0A0K696 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 118 bits (296), Expect = 4.47e-29
Identity = 71/177 (40.11%), Postives = 100/177 (56.50%), Query Frame = 0


Query: 56  NAVFPLSLRRFFHHRPPFHYLLWILSSLDLPPAASHAVARNIAAFVTHLTRGNAEVDLRV 115
           NA+F LSLR+    + P  Y+  ILSSL++ PAAS  +AR IA+ +  +   N+E +  +
Sbjct: 58  NALFYLSLRQL---QSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHI 117

Query: 116 VAVVDYIQIAVSVGQRQRQSRSWNAEESMILDEVPAPEPAPEFV-------EEEEGGELG 175
           +A VD+I++   +           AEE+++LDE P        V        EE+  ELG
Sbjct: 118 LAEVDFIRL---IWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKMEELG 177

Query: 176 DCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPI 225
           DCSIC +EL  +          K +V++I CGHVYH+SCI  WL N NSCPLCR P+
Sbjct: 178 DCSICLDELSCE----------KREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPL 218

[Close]

BLAST of MC01g_new0205 vs. TAIR 10
Match: AT4G05350.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 6.1e-09
Identity = 26/60 (43.33%), Postives = 32/60 (53.33%), Query Frame = 0


Query: 166 ELGDCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPI 225
           E   CSIC E L         SG K   V ++ C HV+H  C++ WL  KN+CPLCR  I
Sbjct: 153 ETEPCSICLESLV--------SGPKPRDVTRMTCSHVFHNGCLLEWLKRKNTCPLCRTEI 204

[Close]

BLAST of MC01g_new0205 vs. TAIR 10
Match: AT4G12190.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.4e-08
Identity = 24/57 (42.11%), Postives = 31/57 (54.39%), Query Frame = 0


Query: 166 ELGDCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCR 223
           E   CSIC E L         SG K   + ++ C HV+H  C++ WL  KN+CPLCR
Sbjct: 18  ETEPCSICLESLV--------SGPKPRDITRMTCSHVFHNGCLLEWLKRKNTCPLCR 66

[Close]

BLAST of MC01g_new0205 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.3e-08
Identity = 28/96 (29.17%), Postives = 42/96 (43.75%), Query Frame = 0


Query: 142 ESMILDEVPAPEPAPE----------FVEEEEGGELGDCSICFEELKIDGHGGNGSGTKK 201
           E +  D+ P P PA E             +    ++  C++C EE  + G          
Sbjct: 186 EQLTQDDRPGPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGG---------- 245

Query: 202 NKVVKIGCGHVYHKSCIITWLTNKNSCPLCRMPITL 228
               ++ C H+YHK CI+ WL   NSCP+CR  + L
Sbjct: 246 -DATELPCKHIYHKDCIVPWLRLNNSCPICRRDLPL 270

[Close]

BLAST of MC01g_new0205 vs. TAIR 10
Match: AT3G30460.1 (RING/U-box superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 6.7e-08
Identity = 31/76 (40.79%), Postives = 40/76 (52.63%), Query Frame = 0


Query: 154 PAPEFVEEE--EGGELGDCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITW 213
           P  EF  EE  E G L  C+IC EEL               ++ ++ C H YHK CI  W
Sbjct: 80  PVVEFTAEEMMERG-LVVCAICREEL-----------AANERLSELPCRHYYHKECISNW 139

Query: 214 LTNKNSCPLCRMPITL 228
           L+N+N+CPLCR  + L
Sbjct: 140 LSNRNTCPLCRHNVEL 143

[Close]

BLAST of MC01g_new0205 vs. TAIR 10
Match: AT4G12140.1 (RING/U-box superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.0e-07
Identity = 21/57 (36.84%), Postives = 30/57 (52.63%), Query Frame = 0


Query: 166 ELGDCSICFEELKIDGHGGNGSGTKKNKVVKIGCGHVYHKSCIITWLTNKNSCPLCR 223
           E   CSIC + L           + K    ++ C HV+H SC++ WL  KN+CP+CR
Sbjct: 149 ETDSCSICLQSL---------VSSSKTGPTRMSCSHVFHSSCLVEWLKRKNTCPMCR 196

The following BLAST results are available for this feature:

BLAST of MC01g_new0205 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P90990	1.5e-07	41.38	E3 ubiquitin-protein ligase trul-1 OS=Caenorhabditis elegans OX=6239 GN=trul-1 P...	[more]
Q9VE61	6.1e-06	30.91	E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN...	[more]
Q08109	3.0e-05	35.71	ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Saccharomyces cerevisiae (st...	[more]
Q9ULK6	3.0e-05	34.33	RING finger protein 150 OS=Homo sapiens OX=9606 GN=RNF150 PE=1 SV=2	[more]
Q5DTZ6	3.0e-05	34.33	RING finger protein 150 OS=Mus musculus OX=10090 GN=Rnf150 PE=2 SV=2	[more]

BLAST of MC01g_new0205 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151639.1	4.62e-162	99.13	uncharacterized protein LOC111019546 [Momordica charantia]	[more]
KAG6595648.1	7.30e-35	35.37	hypothetical protein SDJN03_12201, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023518048.1	7.67e-34	34.93	uncharacterized protein LOC111781594 [Cucurbita pepo subsp. pepo]	[more]
KAG6595650.1	8.09e-34	34.93	hypothetical protein SDJN03_12203, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022151640.1	7.80e-32	41.15	E3 ubiquitin-protein ligase RNF181-like [Momordica charantia]	[more]

BLAST of MC01g_new0205 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DDM9	2.23e-162	99.13	uncharacterized protein LOC111019546 OS=Momordica charantia OX=3673 GN=LOC111019...	[more]
A0A6J1DBR5	3.78e-32	41.15	E3 ubiquitin-protein ligase RNF181-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5D3E1S5	2.26e-29	37.78	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S4DUZ4	2.26e-29	37.78	E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 ...	[more]
A0A0A0K696	4.47e-29	40.11	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 P...	[more]

BLAST of MC01g_new0205 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05350.1	6.1e-09	43.33	RING/U-box superfamily protein	[more]
AT4G12190.1	1.4e-08	42.11	RING/U-box superfamily protein	[more]
AT1G60360.1	2.3e-08	29.17	RING/U-box superfamily protein	[more]
AT3G30460.1	6.7e-08	40.79	RING/U-box superfamily protein	[more]
AT4G12140.1	2.0e-07	36.84	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 170..221 e-value: 4.0E-8 score: 43.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 170..222 score: 12.622055
IPR024766	Zinc finger, RING-H2-type	PFAM	PF12678	zf-rbx1	coord: 169..222 e-value: 3.1E-11 score: 43.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 156..228 e-value: 8.2E-18 score: 65.3
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 133..225
None	No IPR available	PANTHER	PTHR15710:SF137	ZINC FINGER, C3HC4 TYPE (RING FINGER) PROTEIN	coord: 133..225
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 153..228

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g_new0205.1	MC01g_new0205.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding