MC01g_new0204 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0204
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	polygalacturonase inhibitor-like
Location	MC01: 7533162 .. 7534844 (+)
RNA-Seq Expression	MC01g_new0204
Synteny	MC01g_new0204

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTCATTATTATATATATATATTTTTTAAAATTTGCTTTATAAAATGAGGAGATCAAACACAAAGAAAGGAAGAAATCCAAATCCAATAATCTCTCTTTTCTTTTGACTTTGAAATCCTTTTACTATCATCAAATGCGGCCTTAGCTGTCTTCTTGCTTCTACACCAGAAAACACTGCGCCACGTAACACCTTTTTAAATCCAAATCTCTCCAAGATTCCTCATTTTCAACCATGGCTACTCCAAACCTCAAGCTTCCATGGCTCCCCCTCTTCTTCTTCTTCTTCTTCCTTTTTCCCACCTTTATTCTCTCAGAACTCTGCAACCCAAATGACAAAAAAGTCCTCCTCAAAATCAAACAATCCCTCAACAACCCCTACATCCTCACCTCATGGAAGCCCCAAGAAGACTGCTGCACTTGGTACTGCGTCCAATGCCATCCCACTTCTCACCGTATCATTTCCCTGACCATCTTCGCCGACGACCAACTCTCCGGCCAAATCCCGCCGGCGGTCGGCGATCTCCCGTTCCTCGAAACCCTCATGCTCCACAAGCTCCCCAATCTCTCCGGCACCGTCCCCGCCGCCGTCGCTGATCTCCGCAACCTCAAATTCCTCGACCTGAGCAGGAACGGCCTCTCCGGTCCGGTCCCGGAGTTCCTCGGCTCCCTCGCCAATCTCACCTTTCTCGATCTCTCCTTCAACAACTTCACCGGAGCCATCCCGAGCTCTCTCGCCGACCTCCAGCGCCTAGGCGCTCTCCACTTGGACCGCAACAAGTTAACCGGACAAATCCCGCACTCCTTCGGATACTTCAAGGGGGAAATTCCCGACCTTTACCTCTCCCACAACCAACTCTCCGGCGAAATCCCAACTTCCCTGGCCGGAATGGATTTCAACGTCATCGATTTGTCAAGAAACAAGCTCCAAGGCGACGCCTCCATGATTTTCGGCGCGAACAAGACGACCCAGATCGTGGATTTGTCGAGGAATCTGCTGGAGTTCAATTTGACGGCGGTTGTGTTTCCGCAGAGCCTGACTTCGCTGGATCTGAATCATAACAAGATCTTTGGGGGGATTCCGCCGGAGATGACGAAATTGAGTTTGCAGTATTTGAACGTGAGTTATAATAGGCTGTGTGGCGAGATTCCGGTGGGGGGGCAGTTGCAGAGCTTCGATGTTTATGAGTATTTTCATAATAAGTGTTTGTGCGGCGCACCGCTTGGTAGCTGCAAATAATGATGGACTTTTGTTATCTCAATCTTGTGTATTGTGCAATCTTTAATATTAAATAAAGTTTGGTCATTTGAAAAATTTGTTCATCAGTGAGTTGTCGTTCTTATTATCTCAATCTTGTGTATTCTGCAATCTTTAAGAATAAAAAATGACGGACTAGCTTAAATCGCTTTTGAAAACTCTCTGCCTATCGTCTTGTTTTTTGTTCTCGTTCGTGTAGAGGACTTAAATCTATTGAGATTTTTTACCTCAATATGATTATTTGAAGGTTTTTTTAATGGAGCTTAATGAGTCATATTCTCAAGTATGTGCGCAATTTCCTCTTGATGTGTTCTCCTCCCACTCTATCAACAAGATGTTTGCACTAGTTGCTCAAGAAGAACGACAATGTTTTTATTGATATTCATGAACATCAGCAGGGTTACTACTCTTCGAGAGTGAATCAACT

mRNA sequence

ATGGCTACTCCAAACCTCAAGCTTCCATGGCTCCCCCTCTTCTTCTTCTTCTTCTTCCTTTTTCCCACCTTTATTCTCTCAGAACTCTGCAACCCAAATGACAAAAAAGTCCTCCTCAAAATCAAACAATCCCTCAACAACCCCTACATCCTCACCTCATGGAAGCCCCAAGAAGACTGCTGCACTTGGTACTGCGTCCAATGCCATCCCACTTCTCACCGTATCATTTCCCTGACCATCTTCGCCGACGACCAACTCTCCGGCCAAATCCCGCCGGCGGTCGGCGATCTCCCGTTCCTCGAAACCCTCATGCTCCACAAGCTCCCCAATCTCTCCGGCACCGTCCCCGCCGCCGTCGCTGATCTCCGCAACCTCAAATTCCTCGACCTGAGCAGGAACGGCCTCTCCGGTCCGGTCCCGGAGTTCCTCGGCTCCCTCGCCAATCTCACCTTTCTCGATCTCTCCTTCAACAACTTCACCGGAGCCATCCCGAGCTCTCTCGCCGACCTCCAGCGCCTAGGCGCTCTCCACTTGGACCGCAACAAGTTAACCGGACAAATCCCGCACTCCTTCGGATACTTCAAGGGGGAAATTCCCGACCTTTACCTCTCCCACAACCAACTCTCCGGCGAAATCCCAACTTCCCTGGCCGGAATGGATTTCAACGTCATCGATTTGTCAAGAAACAAGCTCCAAGGCGACGCCTCCATGATTTTCGGCGCGAACAAGACGACCCAGATCGTGGATTTGTCGAGGAATCTGCTGGAGTTCAATTTGACGGCGGTTGTGTTTCCGCAGAGCCTGACTTCGCTGGATCTGAATCATAACAAGATCTTTGGGGGGATTCCGCCGGAGATGACGAAATTGAGTTTGCAGTATTTGAACGTGAGTTATAATAGGCTGTGTGGCGAGATTCCGGTGGGGGGGCAGTTGCAGAGCTTCGATGTTTATGAGTATTTTCATAATAAGTGTTTGTGCGGCGCACCGCTTGGTAGCTGCAAATAA

Coding sequence (CDS)

ATGGCTACTCCAAACCTCAAGCTTCCATGGCTCCCCCTCTTCTTCTTCTTCTTCTTCCTTTTTCCCACCTTTATTCTCTCAGAACTCTGCAACCCAAATGACAAAAAAGTCCTCCTCAAAATCAAACAATCCCTCAACAACCCCTACATCCTCACCTCATGGAAGCCCCAAGAAGACTGCTGCACTTGGTACTGCGTCCAATGCCATCCCACTTCTCACCGTATCATTTCCCTGACCATCTTCGCCGACGACCAACTCTCCGGCCAAATCCCGCCGGCGGTCGGCGATCTCCCGTTCCTCGAAACCCTCATGCTCCACAAGCTCCCCAATCTCTCCGGCACCGTCCCCGCCGCCGTCGCTGATCTCCGCAACCTCAAATTCCTCGACCTGAGCAGGAACGGCCTCTCCGGTCCGGTCCCGGAGTTCCTCGGCTCCCTCGCCAATCTCACCTTTCTCGATCTCTCCTTCAACAACTTCACCGGAGCCATCCCGAGCTCTCTCGCCGACCTCCAGCGCCTAGGCGCTCTCCACTTGGACCGCAACAAGTTAACCGGACAAATCCCGCACTCCTTCGGATACTTCAAGGGGGAAATTCCCGACCTTTACCTCTCCCACAACCAACTCTCCGGCGAAATCCCAACTTCCCTGGCCGGAATGGATTTCAACGTCATCGATTTGTCAAGAAACAAGCTCCAAGGCGACGCCTCCATGATTTTCGGCGCGAACAAGACGACCCAGATCGTGGATTTGTCGAGGAATCTGCTGGAGTTCAATTTGACGGCGGTTGTGTTTCCGCAGAGCCTGACTTCGCTGGATCTGAATCATAACAAGATCTTTGGGGGGATTCCGCCGGAGATGACGAAATTGAGTTTGCAGTATTTGAACGTGAGTTATAATAGGCTGTGTGGCGAGATTCCGGTGGGGGGGCAGTTGCAGAGCTTCGATGTTTATGAGTATTTTCATAATAAGTGTTTGTGCGGCGCACCGCTTGGTAGCTGCAAATAA

Protein sequence

MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDCCTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFGANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNRLCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK

Homology

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BLAST of MC01g_new0204 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.3e-129
Identity = 222/309 (71.84%), Postives = 252/309 (81.55%), Query Frame = 0


Query: 26  LSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDCCTWYCVQCHPTSHRIISLTIFADDQ 85
           LS+LCNP+DKKVLL+IK++  +PY+L SWK   DCC WYCV C  T++RI SLTIFA  Q
Sbjct: 23  LSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCDSTTNRINSLTIFA-GQ 82

Query: 86  LSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRNLKFLDLSRNGLSGPVPEFLGS 145
           +SGQIP  VGDLP+LETL  HK PNL+G +  A+A L+ LK L LS   LSG VP+FL  
Sbjct: 83  VSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRLSWTNLSGSVPDFLSQ 142

Query: 146 LANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLTGQIPHSFGYFKGEIPDLYLSH 205
           L NLTFLDLSFNN TGAIPSSL++L  LGAL LDRNKLTG IP SFG F G +PDLYLSH
Sbjct: 143 LKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPISFGQFIGNVPDLYLSH 202

Query: 206 NQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFGANKTTQIVDLSRNLLEFNLTAVVFP 265
           NQLSG IPTS A MDF  IDLSRNKL+GDAS+IFG NKTTQIVDLSRNLLEFNL+ V FP
Sbjct: 203 NQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVDLSRNLLEFNLSKVEFP 262

Query: 266 QSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNRLCGEIPVGGQLQSFDVYEYFHNKCL 325
            SLTSLD+NHNKI+G IP E T+L+ Q+LNVSYNRLCG+IPVGG+LQSFD Y YFHN+CL
Sbjct: 263 TSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVGGKLQSFDEYSYFHNRCL 322

Query: 326 CGAPLGSCK 335
           CGAPL SCK
Sbjct: 323 CGAPLPSCK 330

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BLAST of MC01g_new0204 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 3.8e-121
Identity = 217/334 (64.97%), Postives = 250/334 (74.85%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60
           M T  L L    L        P   LSE CNP DKKVLL+IK++L+NPYIL SW P  DC
Sbjct: 1   METSKLFLLSSSLLLVLLATRPCPSLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDC 60

Query: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120
           C WYCV+C  T+HRI SLTIF+  QLSGQIP AVGDLPFLETL+  KL NL+G +P A+A
Sbjct: 61  CGWYCVECDLTTHRINSLTIFS-GQLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIA 120

Query: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDR 180
            L++LK + LS   LSGPVP F   L NLT+LDLSFNN +G IP SL+ L  LGALHLDR
Sbjct: 121 KLKHLKMVRLSWTNLSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDR 180

Query: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240
           N LTG IP SFG F G  P L+LSHNQLSG+IP S  G D NV+DLSRNKL+GD S+ F 
Sbjct: 181 NHLTGPIPDSFGKFAGSTPGLHLSHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFN 240

Query: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300
           ANK+TQIVD SRNL +F+L+ V FP+SLTSLDL+HNKI G +P  MT L LQ+LNVSYNR
Sbjct: 241 ANKSTQIVDFSRNLFQFDLSRVEFPKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNR 300

Query: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 335
           LCG+IPVGG+LQSFD   YFHN+CLCGAPL SCK
Sbjct: 301 LCGKIPVGGKLQSFDYDSYFHNRCLCGAPLQSCK 333

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BLAST of MC01g_new0204 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.8e-114
Identity = 199/330 (60.30%), Postives = 246/330 (74.55%), Query Frame = 0


Query: 7   KLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDCCTWYCV 66
           K   L L F F FL  T +  +LCN NDK  LLKIK+SLNNPY L SW PQ DCC+WYC+
Sbjct: 3   KTATLCLLFLFTFL-TTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCL 62

Query: 67  QC--HPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRN 126
           +C     +HR+ +LTIF+  Q+SGQIP  VGDLP+LETL+  KL NL+GT+   +A L+N
Sbjct: 63  ECGDATVNHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKN 122

Query: 127 LKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLT 186
           L+ L LS   L+GP+P+F+  L NL FL+LSFN+ +G+IPSSL+ L ++ AL L RNKLT
Sbjct: 123 LRMLRLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLT 182

Query: 187 GQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFGANKT 246
           G IP SFG F G +PDL LSHNQLSG IP SL  +DFN IDLSRNKLQGDASM+FG+NKT
Sbjct: 183 GSIPESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKT 242

Query: 247 TQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNRLCGE 306
           T  +DLSRN+ +F+++ V  P++L  LDLNHN I G IP + T+  LQ+ NVSYN+LCG 
Sbjct: 243 TWSIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGH 302

Query: 307 IPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 335
           IP GG+LQ+FD Y YFHNKCLCGAPL  CK
Sbjct: 303 IPTGGKLQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of MC01g_new0204 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 393.3 bits (1009), Expect = 2.8e-108
Identity = 187/317 (58.99%), Postives = 233/317 (73.50%), Query Frame = 0


Query: 20  LFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDCCTWYCVQC--HPTSHRIIS 79
           L  T +  +LC+ +DK  LLKIK+SLNNPY L SW P+ DCC+WYC++C     +HR+ S
Sbjct: 15  LLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDATVNHRVTS 74

Query: 80  LTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRNLKFLDLSRNGLSG 139
           L I  D ++SGQIPP VGDLP+L +L+  KL NL+G +   +A L+NL FL LS   L+G
Sbjct: 75  L-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLRLSWTNLTG 134

Query: 140 PVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLTGQIPHSFGYFKGE 199
           PVPEFL  L NL ++DLSFN+ +G+IPSSL+ L++L  L L RNKLTG IP SFG F G+
Sbjct: 135 PVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPESFGTFSGK 194

Query: 200 IPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFGANKTTQIVDLSRNLLEF 259
           +P L+LSHNQLSG IP SL   DF  IDLSRNKLQGDAS++FGA KTT IVD+SRN+ +F
Sbjct: 195 VPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVDISRNMFQF 254

Query: 260 NLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNRLCGEIPVGGQLQSFDVY 319
           +L+ V   ++L +LD+NHN I G IP E +K   Q LNVSYNRLCG IP G  +Q FD Y
Sbjct: 255 DLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPKGEYIQRFDSY 314

Query: 320 EYFHNKCLCGAPLGSCK 335
            +FHNKCLCGAPL SCK
Sbjct: 315 SFFHNKCLCGAPLPSCK 330

[Close]

BLAST of MC01g_new0204 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.9e-91
Identity = 166/322 (51.55%), Postives = 219/322 (68.01%), Query Frame = 0


Query: 13  LFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDCCT-WYCVQCHPT 72
           L  FF  LF T   S  C  NDK  LL+IK++L NP +L+SW P+ DCCT W  V+C  T
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 73  SHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRNLKFLDLS 132
           + R+  L++    ++SGQI   +GDL  L TL    LP+L+G +P  +  L+NL  L L 
Sbjct: 67  NRRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 133 RNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLTGQIPHSF 192
              LSGP+P+++  L +LTFLDLSFN FTG IP SL+ + +L A+ ++ NKLTG IP+SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 193 GYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFGANKTTQIVDLS 252
           G F G +P+LYLS+N+LSG+IP SL+  DFN +DLS N  +GDA M FG NKTT  VDLS
Sbjct: 187 GSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLS 246

Query: 253 RNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNRLCGEIPVGGQL 312
           RN+  F+L  V F +S+ SLDL+ N I+G IPP +TKL L++ NVS N LCG+IP GG L
Sbjct: 247 RNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLL 306

Query: 313 QSFDVYEYFHNKCLCGAPLGSC 334
           Q+F+   + HN CLCG PL +C
Sbjct: 307 QTFEPSAFAHNICLCGTPLKAC 325

[Close]

BLAST of MC01g_new0204 vs. NCBI nr
Match: XP_022151649.1 (polygalacturonase inhibitor-like [Momordica charantia])

HSP 1 Score: 687 bits (1772), Expect = 1.53e-249
Identity = 333/334 (99.70%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60
           MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120
           CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDR 180
           DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGA+PSSLADLQRLGALHLDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240
           NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300
           ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

Query: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK
Sbjct: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334

[Close]

BLAST of MC01g_new0204 vs. NCBI nr
Match: ARM53421.1 (pgip2 [Momordica charantia])

HSP 1 Score: 685 bits (1767), Expect = 8.84e-249
Identity = 332/334 (99.40%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60
           MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120
           CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDR 180
           DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGA+PSSLADLQRLGALHLDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240
           NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300
           ANKTTQIVDLSRNLLEF+LTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFSLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

Query: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK
Sbjct: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334

[Close]

BLAST of MC01g_new0204 vs. NCBI nr
Match: XP_022151650.1 (polygalacturonase inhibitor-like [Momordica charantia] >ARM53420.1 pgip1 [Momordica charantia])

HSP 1 Score: 552 bits (1423), Expect = 1.88e-196
Identity = 266/334 (79.64%), Postives = 296/334 (88.62%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60
           MA P L       FFF  F+  +   +ELCNPNDKKVLLKIK+S NNPYILTSWKP+EDC
Sbjct: 1   MAAPKLT------FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDC 60

Query: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120
           C+WYCVQCHPTSHRIISLTIFADD+LSGQIPP VGDLPFLETLMLHKLPNL+G +PAAVA
Sbjct: 61  CSWYCVQCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVA 120

Query: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDR 180
           +L NLK LDLS NGLSGPVPEFLGSL++LT+LDLSFNN TG+IPSSLA+LQRLGALHLDR
Sbjct: 121 NLHNLKSLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDR 180

Query: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240
           NKLTG IPHSFGYFKG+IP LYLSHNQLSG+IP SL  ++FNVIDLSRNKL+GDAS+IFG
Sbjct: 181 NKLTGPIPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFG 240

Query: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300
            NKTT+IVDLSRNLLEF+L+ VVFP+S+T LDLNHNKIFGGIP ++ +L LQ LNVSYNR
Sbjct: 241 GNKTTEIVDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNR 300

Query: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           LCG IPVGG+LQ FDVYEYFHNKCLCG PLGSCK
Sbjct: 301 LCGRIPVGGRLQRFDVYEYFHNKCLCGKPLGSCK 328

[Close]

BLAST of MC01g_new0204 vs. NCBI nr
Match: XP_008452081.1 (PREDICTED: polygalacturonase inhibitor-like [Cucumis melo] >TYK16665.1 polygalacturonase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 509 bits (1311), Expect = 2.96e-179
Identity = 249/337 (73.89%), Postives = 279/337 (82.79%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFF--FFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQE 60
           MA+   KL WLP FF  FFFF F T   +ELCNPNDKKVLLKIK++ NNPYILTSW PQ 
Sbjct: 1   MASNLNKLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQT 60

Query: 61  DCCTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAA 120
           DCC WYCV+C  T+HRIISLTIFADD+L+GQIPP VGDLPFL+TLMLHKLPNL+G +   
Sbjct: 61  DCCHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 120

Query: 121 VADLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHL 180
           +A L NLKFLDLS NGLSG +P+ L +L NL  L LSFN  TG IPSSL++L  LG L L
Sbjct: 121 IAKLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRL 180

Query: 181 DRNKLTGQIPHSFGYFKGE-IPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASM 240
           DRN+LTGQIP SFGYF GE  PDL LSHNQLSG+IP+SL+ ++F  IDLSRNKL+GDA M
Sbjct: 181 DRNQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFM 240

Query: 241 IFGANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVS 300
            FGANKT QI+DLSRNLLEFNL+ VVFPQSLTSLDLNHNK+FGG+PP MT+L LQYLNVS
Sbjct: 241 FFGANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVS 300

Query: 301 YNRLCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           YNRLCG IPVGG LQSFD+Y YFHNKCLCGAPLG CK
Sbjct: 301 YNRLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 337

[Close]

BLAST of MC01g_new0204 vs. NCBI nr
Match: KAA0044799.1 (polygalacturonase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 506 bits (1303), Expect = 4.07e-178
Identity = 247/335 (73.73%), Postives = 277/335 (82.69%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60
           MA+   KL WLP FFFFF    T   +ELCNPNDKKVLLKIK++ NNPYILTSW PQ DC
Sbjct: 1   MASNLNKLSWLPFFFFFFI---TISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDC 60

Query: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120
           C WYCV+C  T+HRIISLTIFADD+L+GQIPP VGDLPFL+TLMLHKLPNL+G +   +A
Sbjct: 61  CHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIA 120

Query: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDR 180
            L NLKFLDLS NGLSG +P+ L +L NL  L LSFN  TG IPSSL++L  LG L LDR
Sbjct: 121 KLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDR 180

Query: 181 NKLTGQIPHSFGYFKGE-IPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIF 240
           N+LTGQIP SFGYF GE  PDL LSHNQLSG+IP+SL+ ++F  IDLSRNKL+GDA M F
Sbjct: 181 NQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFF 240

Query: 241 GANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYN 300
           GANKT QI+DLSRNLLEFNL+ VVFPQSLTSLDLNHNK+FGG+PP MT+L LQYLNVSYN
Sbjct: 241 GANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYN 300

Query: 301 RLCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           RLCG IPVGG LQSFD+Y YFHNKCLCGAPLG CK
Sbjct: 301 RLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 332

[Close]

BLAST of MC01g_new0204 vs. ExPASy TrEMBL
Match: A0A6J1DDN9 (polygalacturonase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111019558 PE=4 SV=1)

HSP 1 Score: 687 bits (1772), Expect = 7.40e-250
Identity = 333/334 (99.70%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60
           MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120
           CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDR 180
           DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGA+PSSLADLQRLGALHLDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240
           NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300
           ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

Query: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK
Sbjct: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334

[Close]

BLAST of MC01g_new0204 vs. ExPASy TrEMBL
Match: A0A1W6IYI7 (Pgip2 OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 685 bits (1767), Expect = 4.28e-249
Identity = 332/334 (99.40%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60
           MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120
           CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDR 180
           DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGA+PSSLADLQRLGALHLDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240
           NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300
           ANKTTQIVDLSRNLLEF+LTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFSLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

Query: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK
Sbjct: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334

[Close]

BLAST of MC01g_new0204 vs. ExPASy TrEMBL
Match: A0A1W6IYG8 (Pgip1 OS=Momordica charantia OX=3673 GN=LOC111019559 PE=2 SV=1)

HSP 1 Score: 552 bits (1423), Expect = 9.10e-197
Identity = 266/334 (79.64%), Postives = 296/334 (88.62%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60
           MA P L       FFF  F+  +   +ELCNPNDKKVLLKIK+S NNPYILTSWKP+EDC
Sbjct: 1   MAAPKLT------FFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDC 60

Query: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120
           C+WYCVQCHPTSHRIISLTIFADD+LSGQIPP VGDLPFLETLMLHKLPNL+G +PAAVA
Sbjct: 61  CSWYCVQCHPTSHRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVA 120

Query: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDR 180
           +L NLK LDLS NGLSGPVPEFLGSL++LT+LDLSFNN TG+IPSSLA+LQRLGALHLDR
Sbjct: 121 NLHNLKSLDLSWNGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDR 180

Query: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240
           NKLTG IPHSFGYFKG+IP LYLSHNQLSG+IP SL  ++FNVIDLSRNKL+GDAS+IFG
Sbjct: 181 NKLTGPIPHSFGYFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFG 240

Query: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300
            NKTT+IVDLSRNLLEF+L+ VVFP+S+T LDLNHNKIFGGIP ++ +L LQ LNVSYNR
Sbjct: 241 GNKTTEIVDLSRNLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVELELQLLNVSYNR 300

Query: 301 LCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           LCG IPVGG+LQ FDVYEYFHNKCLCG PLGSCK
Sbjct: 301 LCGRIPVGGRLQRFDVYEYFHNKCLCGKPLGSCK 328

[Close]

BLAST of MC01g_new0204 vs. ExPASy TrEMBL
Match: A0A5D3CYT3 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004960 PE=4 SV=1)

HSP 1 Score: 509 bits (1311), Expect = 1.43e-179
Identity = 249/337 (73.89%), Postives = 279/337 (82.79%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFF--FFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQE 60
           MA+   KL WLP FF  FFFF F T   +ELCNPNDKKVLLKIK++ NNPYILTSW PQ 
Sbjct: 1   MASNLNKLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQT 60

Query: 61  DCCTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAA 120
           DCC WYCV+C  T+HRIISLTIFADD+L+GQIPP VGDLPFL+TLMLHKLPNL+G +   
Sbjct: 61  DCCHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 120

Query: 121 VADLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHL 180
           +A L NLKFLDLS NGLSG +P+ L +L NL  L LSFN  TG IPSSL++L  LG L L
Sbjct: 121 IAKLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRL 180

Query: 181 DRNKLTGQIPHSFGYFKGE-IPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASM 240
           DRN+LTGQIP SFGYF GE  PDL LSHNQLSG+IP+SL+ ++F  IDLSRNKL+GDA M
Sbjct: 181 DRNQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFM 240

Query: 241 IFGANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVS 300
            FGANKT QI+DLSRNLLEFNL+ VVFPQSLTSLDLNHNK+FGG+PP MT+L LQYLNVS
Sbjct: 241 FFGANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVS 300

Query: 301 YNRLCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           YNRLCG IPVGG LQSFD+Y YFHNKCLCGAPLG CK
Sbjct: 301 YNRLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 337

[Close]

BLAST of MC01g_new0204 vs. ExPASy TrEMBL
Match: A0A1S3BU59 (polygalacturonase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103493193 PE=4 SV=1)

HSP 1 Score: 509 bits (1311), Expect = 1.43e-179
Identity = 249/337 (73.89%), Postives = 279/337 (82.79%), Query Frame = 0


Query: 1   MATPNLKLPWLPLFF--FFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQE 60
           MA+   KL WLP FF  FFFF F T   +ELCNPNDKKVLLKIK++ NNPYILTSW PQ 
Sbjct: 1   MASNLNKLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQT 60

Query: 61  DCCTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAA 120
           DCC WYCV+C  T+HRIISLTIFADD+L+GQIPP VGDLPFL+TLMLHKLPNL+G +   
Sbjct: 61  DCCHWYCVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPT 120

Query: 121 VADLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHL 180
           +A L NLKFLDLS NGLSG +P+ L +L NL  L LSFN  TG IPSSL++L  LG L L
Sbjct: 121 IAKLHNLKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRL 180

Query: 181 DRNKLTGQIPHSFGYFKGE-IPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASM 240
           DRN+LTGQIP SFGYF GE  PDL LSHNQLSG+IP+SL+ ++F  IDLSRNKL+GDA M
Sbjct: 181 DRNQLTGQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFM 240

Query: 241 IFGANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVS 300
            FGANKT QI+DLSRNLLEFNL+ VVFPQSLTSLDLNHNK+FGG+PP MT+L LQYLNVS
Sbjct: 241 FFGANKTIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVS 300

Query: 301 YNRLCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 334
           YNRLCG IPVGG LQSFD+Y YFHNKCLCGAPLG CK
Sbjct: 301 YNRLCGPIPVGGNLQSFDLYSYFHNKCLCGAPLGDCK 337

[Close]

BLAST of MC01g_new0204 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 412.1 bits (1058), Expect = 4.1e-115
Identity = 199/330 (60.30%), Postives = 246/330 (74.55%), Query Frame = 0


Query: 7   KLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDCCTWYCV 66
           K   L L F F FL  T +  +LCN NDK  LLKIK+SLNNPY L SW PQ DCC+WYC+
Sbjct: 3   KTATLCLLFLFTFL-TTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCL 62

Query: 67  QC--HPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRN 126
           +C     +HR+ +LTIF+  Q+SGQIP  VGDLP+LETL+  KL NL+GT+   +A L+N
Sbjct: 63  ECGDATVNHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKN 122

Query: 127 LKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLT 186
           L+ L LS   L+GP+P+F+  L NL FL+LSFN+ +G+IPSSL+ L ++ AL L RNKLT
Sbjct: 123 LRMLRLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLT 182

Query: 187 GQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFGANKT 246
           G IP SFG F G +PDL LSHNQLSG IP SL  +DFN IDLSRNKLQGDASM+FG+NKT
Sbjct: 183 GSIPESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKT 242

Query: 247 TQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNRLCGE 306
           T  +DLSRN+ +F+++ V  P++L  LDLNHN I G IP + T+  LQ+ NVSYN+LCG 
Sbjct: 243 TWSIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGH 302

Query: 307 IPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 335
           IP GG+LQ+FD Y YFHNKCLCGAPL  CK
Sbjct: 303 IPTGGKLQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of MC01g_new0204 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 393.3 bits (1009), Expect = 2.0e-109
Identity = 187/317 (58.99%), Postives = 233/317 (73.50%), Query Frame = 0


Query: 20  LFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDCCTWYCVQC--HPTSHRIIS 79
           L  T +  +LC+ +DK  LLKIK+SLNNPY L SW P+ DCC+WYC++C     +HR+ S
Sbjct: 15  LLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGDATVNHRVTS 74

Query: 80  LTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRNLKFLDLSRNGLSG 139
           L I  D ++SGQIPP VGDLP+L +L+  KL NL+G +   +A L+NL FL LS   L+G
Sbjct: 75  L-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLRLSWTNLTG 134

Query: 140 PVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLTGQIPHSFGYFKGE 199
           PVPEFL  L NL ++DLSFN+ +G+IPSSL+ L++L  L L RNKLTG IP SFG F G+
Sbjct: 135 PVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPESFGTFSGK 194

Query: 200 IPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFGANKTTQIVDLSRNLLEF 259
           +P L+LSHNQLSG IP SL   DF  IDLSRNKLQGDAS++FGA KTT IVD+SRN+ +F
Sbjct: 195 VPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVDISRNMFQF 254

Query: 260 NLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNRLCGEIPVGGQLQSFDVY 319
           +L+ V   ++L +LD+NHN I G IP E +K   Q LNVSYNRLCG IP G  +Q FD Y
Sbjct: 255 DLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPKGEYIQRFDSY 314

Query: 320 EYFHNKCLCGAPLGSCK 335
            +FHNKCLCGAPL SCK
Sbjct: 315 SFFHNKCLCGAPLPSCK 330

[Close]

BLAST of MC01g_new0204 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 335.5 bits (859), Expect = 4.9e-92
Identity = 166/322 (51.55%), Postives = 219/322 (68.01%), Query Frame = 0


Query: 13  LFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDCCT-WYCVQCHPT 72
           L  FF  LF T   S  C  NDK  LL+IK++L NP +L+SW P+ DCCT W  V+C  T
Sbjct: 7   LSIFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--T 66

Query: 73  SHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRNLKFLDLS 132
           + R+  L++    ++SGQI   +GDL  L TL    LP+L+G +P  +  L+NL  L L 
Sbjct: 67  NRRVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLK 126

Query: 133 RNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLTGQIPHSF 192
              LSGP+P+++  L +LTFLDLSFN FTG IP SL+ + +L A+ ++ NKLTG IP+SF
Sbjct: 127 HTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSF 186

Query: 193 GYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFGANKTTQIVDLS 252
           G F G +P+LYLS+N+LSG+IP SL+  DFN +DLS N  +GDA M FG NKTT  VDLS
Sbjct: 187 GSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLS 246

Query: 253 RNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNRLCGEIPVGGQL 312
           RN+  F+L  V F +S+ SLDL+ N I+G IPP +TKL L++ NVS N LCG+IP GG L
Sbjct: 247 RNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLL 306

Query: 313 QSFDVYEYFHNKCLCGAPLGSC 334
           Q+F+   + HN CLCG PL +C
Sbjct: 307 QTFEPSAFAHNICLCGTPLKAC 325

[Close]

BLAST of MC01g_new0204 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-49
Identity = 113/343 (32.94%), Postives = 176/343 (51.31%), Query Frame = 0


Query: 30  CNPNDKKVLLKIKQSLNNPY--ILTSWKPQEDCC-TWYCVQCHPTSHRIISLTIFADDQ- 89
           C+P D+  L   K SL+ P   I  +W    DCC  WY + C P S R+  +++  + + 
Sbjct: 27  CSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWYGISCDPDSGRVTDISLRGESED 86

Query: 90  -----------LSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLRNLKFLDLSRNG 149
                      +SG I PAV DL  L +L+L     ++G +P  +  L +L+ LDL+ N 
Sbjct: 87  AIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGITGEIPPCITSLASLRILDLAGNK 146

Query: 150 LSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKLTGQIPHSFGYF 209
           ++G +P  +G L+ L  L+L+ N  +G IP+SL  L  L  L L  N +TG IP  FG  
Sbjct: 147 ITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIELKHLELTENGITGVIPADFGSL 206

Query: 210 KGEIPDLYLSHNQLSGEIPTSLAGMD-FNVIDLSRNKLQG-----------------DAS 269
           K  +  + L  N+L+G IP S++GM+    +DLS+N ++G                 D +
Sbjct: 207 K-MLSRVLLGRNELTGSIPESISGMERLADLDLSKNHIEGPIPEWMGNMKVLSLLNLDCN 266

Query: 270 MIFG-------ANKTTQIVDLSRNLLEFNLTAVVFPQS-LTSLDLNHNKIFGGIPPEMTK 329
            + G       +N    + +LSRN LE  +  V   ++ L SLDL+HN + G IP  ++ 
Sbjct: 267 SLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKTYLVSLDLSHNSLSGRIPDSLSS 326

Query: 330 LS-LQYLNVSYNRLCGEIPVGGQLQSFDVYEYFHNKCLCGAPL 331
              + +L++S+N+LCG IP G      +   +  N+CLCG PL
Sbjct: 327 AKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQCLCGGPL 368

[Close]

BLAST of MC01g_new0204 vs. TAIR 10
Match: AT5G12940.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 185.3 bits (469), Expect = 8.2e-47
Identity = 119/359 (33.15%), Postives = 180/359 (50.14%), Query Frame = 0


Query: 18  FFLFPTFILSELCNPNDKKVLLKIKQSLNNPYI--LTSWKPQEDCCTWYCVQCHPTSHRI 77
           F L  T + S  C P+D+  LL+ +  LN PYI    +WK  + C  WY V C P + R+
Sbjct: 17  FLLLSTTVHS--CLPSDRAALLEFRAKLNEPYIGVFNTWKGLDCCKGWYGVSCDPNTRRV 76

Query: 78  ISLTIFADDQ------------LSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVADLR 137
             +T+  + +            ++G I P++  L  L  +++     +SG +P+ + +L 
Sbjct: 77  AGITLRGESEDPLFQKAKRSGLMTGSISPSICKLTRLSGIIIADWKGISGVIPSCIENLP 136

Query: 138 NLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAIPSSLADLQRLGALHLDRNKL 197
            L+ LDL  N  SG +P  +G L  L  L+L+ N+  G IP S+  L  L  L L  N +
Sbjct: 137 FLRHLDLVGNKFSGVIPANIGKLLRLKVLNLADNHLYGVIPPSITRLVSLSHLDLRNNNI 196

Query: 198 TGQIPHSFGYFK-------------GEIP----------DLYLSHNQLSGEIPTSLAGMD 257
           +G IP   G  K             G+IP          DL LS N+L+G IP S   M 
Sbjct: 197 SGVIPRDIGRLKMVSRVLLSGNKISGQIPDSLTRIYRLADLELSMNRLTGPIPASFGKMS 256

Query: 258 -FNVIDLSRNKLQGDASMIFGANKTTQI--VDLSRNLLEFNLTAVVFPQS-LTSLDLNHN 317
               ++L  N + G   MI G+   + I  ++LS NL+  ++     P+S  T LDL +N
Sbjct: 257 VLATLNLDGNLISG---MIPGSLLASSISNLNLSGNLITGSIPNTFGPRSYFTVLDLANN 316

Query: 318 KIFGGIPPEMTKLS-LQYLNVSYNRLCGEIPVGGQLQSFDVYEYFHNKCLCGAPLGSCK 335
           ++ G IP  +T  S + +L+VS+N LCG+IP+G      D   + +N CLCG PLG+CK
Sbjct: 317 RLQGPIPASITAASFIGHLDVSHNHLCGKIPMGSPFDHLDATSFAYNACLCGKPLGNCK 370

The following BLAST results are available for this feature:

BLAST of MC01g_new0204 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05091	1.3e-129	71.84	Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1	[more]
A7PW81	3.8e-121	64.97	Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1	[more]
Q9M5J9	5.8e-114	60.30	Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1	[more]
Q9M5J8	2.8e-108	58.99	Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2	[more]
Q9LH52	6.9e-91	51.55	Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ...	[more]

BLAST of MC01g_new0204 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151649.1	1.53e-249	99.70	polygalacturonase inhibitor-like [Momordica charantia]	[more]
ARM53421.1	8.84e-249	99.40	pgip2 [Momordica charantia]	[more]
XP_022151650.1	1.88e-196	79.64	polygalacturonase inhibitor-like [Momordica charantia] >ARM53420.1 pgip1 [Momord...	[more]
XP_008452081.1	2.96e-179	73.89	PREDICTED: polygalacturonase inhibitor-like [Cucumis melo] >TYK16665.1 polygalac...	[more]
KAA0044799.1	4.07e-178	73.73	polygalacturonase inhibitor-like [Cucumis melo var. makuwa]	[more]

BLAST of MC01g_new0204 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DDN9	7.40e-250	99.70	polygalacturonase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111019558 ...	[more]
A0A1W6IYI7	4.28e-249	99.40	Pgip2 OS=Momordica charantia OX=3673 PE=2 SV=1	[more]
A0A1W6IYG8	9.10e-197	79.64	Pgip1 OS=Momordica charantia OX=3673 GN=LOC111019559 PE=2 SV=1	[more]
A0A5D3CYT3	1.43e-179	73.89	Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BU59	1.43e-179	73.89	polygalacturonase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103493193 PE=4 SV...	[more]

BLAST of MC01g_new0204 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06860.1	4.1e-115	60.30	polygalacturonase inhibiting protein 1	[more]
AT5G06870.1	2.0e-109	58.99	polygalacturonase inhibiting protein 2	[more]
AT3G12145.1	4.9e-92	51.55	Leucine-rich repeat (LRR) family protein	[more]
AT3G12610.1	1.4e-49	32.94	Leucine-rich repeat (LRR) family protein	[more]
AT5G12940.1	8.2e-47	33.15	Leucine-rich repeat (LRR) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 28..334 e-value: 2.5E-85 score: 288.4
IPR013210	Leucine-rich repeat-containing N-terminal, plant-type	PFAM	PF08263	LRRNT_2	coord: 32..68 e-value: 1.2E-9 score: 38.3
IPR001611	Leucine-rich repeat	PFAM	PF13516	LRR_6	coord: 266..278 e-value: 0.37 score: 11.0 coord: 147..162 e-value: 0.45 score: 10.7
IPR001611	Leucine-rich repeat	PFAM	PF00560	LRR_1	coord: 124..143 e-value: 1.2 score: 10.0
None	No IPR available	PANTHER	PTHR48059:SF10	POLYGALACTURONASE INHIBITOR 2	coord: 13..333
None	No IPR available	PANTHER	PTHR48059	POLYGALACTURONASE INHIBITOR 1	coord: 13..333
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 29..332

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g_new0204.1	MC01g_new0204.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0090353	polygalacturonase inhibitor activity
molecular_function	GO:0005515	protein binding