Homology
BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match:
F4JT76 (Vacuolar protein sorting-associated protein 54, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VPS54 PE=1 SV=1)
HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 670/1113 (60.20%), Postives = 807/1113 (72.51%), Query Frame = 0
Query: 1 MESQPSQSGRSPT---EYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGK- 60
M+S PS GRS T S L R +S ++ S K+ SDASSQSLSSILNNPH GK
Sbjct: 1 MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60
Query: 61 ----SDASWAAWWSSSSTVSPPEFLPLSST-IASSEVTRFDFNNYIALISDSYHRFEDIR 120
SDASW WWSSS+ V+P EF P++ST + SE+TR DF+ Y++ IS+S+ RFEDIR
Sbjct: 61 GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120
Query: 121 NHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQ 180
H+ +ES G D L ACLREVP+LYFKEDFALEDGATFR+ACPF ++ +NL LQ
Sbjct: 121 KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180
Query: 181 EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDL 240
EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR+LKETIRLLD +L
Sbjct: 181 EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240
Query: 241 VDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLL 300
VDSAR+IQE ++TR N+L LQ+KL+LILYVNQA+SALKLLVAS DCAGALD+TDDL +LL
Sbjct: 241 VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300
Query: 301 EGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLM 360
GDEL+GL+CFRHLRDHV +SI+SI SIL++EFMR S HD G++D++I+ R S
Sbjct: 301 AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360
Query: 361 NGK--DEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAE 420
NG +EVK +EE+ S DRLLP++IGLLRTAK PS+LR+YRD +T++MK AIK AVA+
Sbjct: 361 NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420
Query: 421 LLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRA 480
LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AIFKIVQ HLVRA
Sbjct: 421 LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480
Query: 481 AEVKKSIEWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVT 540
+EVKK+IEWI+CN+DGHYAADSVAAAIA+GA AA TAQ+ Q G L+ + +K
Sbjct: 481 SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540
Query: 541 SLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVH 600
L GK++DA++ NMSRNFR
Sbjct: 541 PLQGKSSDASSLMNMSRNFR---------------------------------------- 600
Query: 601 IILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKL 660
ADVLRENTEAVFAAC+ HGRWAKL
Sbjct: 601 -----------------------------------ADVLRENTEAVFAACEVTHGRWAKL 660
Query: 661 LGVRILVHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESR 720
LGVR L+HPKL+LQEF+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESR
Sbjct: 661 LGVRALLHPKLKLQEFMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESR 720
Query: 721 MTKIKAVLDQETWVEVDVPDEFQAIAESLY-SQELLSAKIDDAQGNMDRSYTDVTTNNDD 780
MTK+KAVLDQETW E+DVP+EFQ+I SL+ SQ L+S K+DDA N SY +
Sbjct: 721 MTKLKAVLDQETWDEIDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNG 780
Query: 781 SSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPA-DKIEKSKADVLIPTTQINNTNVK 840
S G G N++ +E ++SS+ + + KPT + + +E+SKA V T N +N K
Sbjct: 781 SLTSGSGDQNSELRNEKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQK 840
Query: 841 ERGKSSSQTLQYKGIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNT 900
GKS+ L Y+G+GYHM MLSEYIDMN+SLPALSSE+V RVVE+L+FFNT
Sbjct: 841 AHGKSN---LFYQGVGYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNT 900
Query: 901 RTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLIS 960
RTCQLVLGAGAMQVSGLKSI +KHLALASQVI FT IIPE RRILF KVPE RK LL
Sbjct: 901 RTCQLVLGAGAMQVSGLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSV 960
Query: 961 EIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLT 1020
EID+VAQD+++HRDEI+TKLVQIMRERLL HL GLP++VE WNR D++ Q +FA LT
Sbjct: 961 EIDKVAQDFRIHRDEIYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLT 1020
Query: 1021 KEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHIL 1080
+EVGYL RVLS TLHEADVQAIF+QV+ I H Q S+ + L+IS+ +AK RL V+ IL
Sbjct: 1021 REVGYLHRVLSETLHEADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELIL 1028
Query: 1081 GCIRSLPCDDLSKPDTPNWGQLDEFLDQRFGSE 1089
CIRSLP D+ ++ D PNWGQLDEF + F E
Sbjct: 1081 KCIRSLPSDNANQSDIPNWGQLDEFFAEHFREE 1028
BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match:
Q9JMK8 (Vacuolar protein sorting-associated protein 54 OS=Rattus norvegicus OX=10116 GN=Vps54 PE=2 SV=1)
HSP 1 Score: 228.8 bits (582), Expect = 3.0e-58
Identity = 239/929 (25.73%), Postives = 395/929 (42.52%), Query Frame = 0
Query: 132 LREVPALYFKEDFALEDGATFRAACPF-----------LNVTQNLVLQEKLSHYLDVVEL 191
L +VP ++ K DFAL+D TF + P+ + + +LQEKLSHYLD+VE+
Sbjct: 147 LEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEV 206
Query: 192 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 251
++ +ISLRS +FF A +L + + ++ L++ I +D + + + +I
Sbjct: 207 NIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLQILRLAL 266
Query: 252 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 311
TRNN + + KLKL+ V+Q +++L+++ + GALD+ +L+ EL G+H FR
Sbjct: 267 TRNNCVKVSNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQ-QELQGVHSFR 326
Query: 312 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 371
HL + + I ++ AEF S D + + G+ +V EEE
Sbjct: 327 HLGSQLCELEKLIDKMMIAEFSTYSHSD---------------LNRPLEGECQVL--EEE 386
Query: 372 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 431
RL+ ++ GLL KL + L +Y + K IK V + V + +D+D
Sbjct: 387 ------RLVSLVFGLLEQRKL-NFLEIYGEETIITAKNIIKECV--INKVAQVEEIDTDA 446
Query: 432 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCN- 491
LA ++R L+ ++ LL IF + L R I ++ +
Sbjct: 447 V------------VKLADQMRMLNFPQWIDLLKDIFSKFTIFLQRVKATLNVIHSVVLSV 506
Query: 492 LDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPS 551
LD + + +AG D +V L + + + AA +
Sbjct: 507 LDKNQRTRELEEVSQ--QRSAGKDNSLDTEVAYL---THEGLFISDAFSEAEPASAAVDT 566
Query: 552 NMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLA 611
RN T P S E C S D
Sbjct: 567 TSQRN-----------TSPHS-----EPC---------SSDSVSEPECTTDSSSSKEQTP 626
Query: 612 LSFPLSAIILVEKEEMFERWKKADVLRENTEAVFA-ACDAAHGRWAKLLGVRIL--VHPK 671
S L + ++ E+M + L N + + A D H R K L R K
Sbjct: 627 ASATLGGVDIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEK 686
Query: 672 LRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQ 731
L EF+++ + + +I TE+I GR S+ G LQSQA FV+ HE R TK+ +LD
Sbjct: 687 LNSTEFIALSRLMETYIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDN 746
Query: 732 ETWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNA 791
E W + DVP EFQ + +S+ A G +
Sbjct: 747 ERWKQADVPAEFQDLVDSI------------ADGKI------------------------ 806
Query: 792 QQHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQY 851
L D PA ++ ADVL+ + QY
Sbjct: 807 ----ALPDKK-------------PAATEDRKPADVLV-----------------VEGHQY 866
Query: 852 KGIGYHMML----SEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGL 911
+G ++L EY D++P+++++++ R+ ++LK+FN+R+CQLVLGAGA+QV GL
Sbjct: 867 AVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRSCQLVLGAGALQVVGL 922
Query: 912 KSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEIH 971
K+IT+K+LAL+S+ + IP IR ++P + +LL D + +DY H EI
Sbjct: 927 KTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPRKQWSLL-RHFDHITKDYHDHIGEIS 922
Query: 972 TKLVQIMR---ERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTL 1031
+KL+ IM ++LL +R + P PS R++ K++ + + L
Sbjct: 987 SKLIAIMDSLFDKLL-------------SRCEVEAPAPSPCFRNICKQMTKMHEAIFDLL 922
Query: 1032 HEADVQAIFKQVVKIFHLQISEAFSRLDI 1039
+ Q + ++ + + + S L++
Sbjct: 1047 PKEQTQMLILRINASYKFHLKKQLSHLNV 922
BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match:
Q9P1Q0 (Vacuolar protein sorting-associated protein 54 OS=Homo sapiens OX=9606 GN=VPS54 PE=1 SV=2)
HSP 1 Score: 228.0 bits (580), Expect = 5.1e-58
Identity = 260/1026 (25.34%), Postives = 430/1026 (41.91%), Query Frame = 0
Query: 38 SSQSLSSILNNPHAGK--SDASWAAWWSS--SSTVSPPEFLPLSSTIASSEVTRFDFNNY 97
S +L + LN+P K SD W + V P +LP ++++ F Y
Sbjct: 69 SKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLP--------QISKEHFTVY 128
Query: 98 IALISDSYHRFEDIRNHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFR 157
IS E +N + ++ + L +VP ++ K DFAL+D TF
Sbjct: 129 QQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFN 188
Query: 158 AACPF-----------LNVTQNLVLQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQD 217
+ P+ + + +LQEKLSHYLD+VE+++ +ISLRS +FF A +
Sbjct: 189 SVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHE 248
Query: 218 LNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNATRNNLLALQQKLKLILYVNQAI 277
L + + ++ L++ I +D + + + I TRNN + + KLKL+ V+Q
Sbjct: 249 LQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQ 308
Query: 278 SALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFRHLRDHVAASIESITSILSAEFM 337
+++L+++ + GALD+ +L+ EL G+H FRHL + + I ++ AEF
Sbjct: 309 PTVQVLLSTSEFVGALDLIATTQEVLQ-QELQGIHSFRHLGSQLCELEKLIDKMMIAEF- 368
Query: 338 RASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEEASNFRDRLLPIIIGLLRTAKLP 397
S + D++ + +D+ + EEE RL+ ++ GLL+ KL
Sbjct: 369 --STYSHSDLNRPL--------------EDDCQVLEEE------RLISLVFGLLKQRKL- 428
Query: 398 SVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRG 457
+ L +Y + + K IK V + V +D+D LA ++R
Sbjct: 429 NFLEIYGEKMVITAKNIIKQCV--INKVSQTEEIDTDVV------------VKLADQMRM 488
Query: 458 LSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCN-LDGHYAADSVAAAIAIGAAAAG 517
L+ + LL IF + L R I ++ + LD + + AA
Sbjct: 489 LNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKD 548
Query: 518 TAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSF 577
+ D++ L+ + A L A D + N S N C
Sbjct: 549 NSLDTE-VAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPC------------- 608
Query: 578 GGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLALSFPLSAIILVEKEEMFERWKK 637
SS + T + + P I+V ++ +
Sbjct: 609 ---------------SSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSEL 668
Query: 638 ADVLRENTEAVFAACDAAHGRWAKLLGVRIL--VHPKLRLQEFLSIYNITQDFITATEKI 697
+ E +++A D H R K L R KL EF+++ + + FI TE+I
Sbjct: 669 GKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQI 728
Query: 698 GGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQAIAESLYSQE 757
GR S+ G LQSQA FV+ HE R TK+ +LD E W + DVP EFQ + +SL
Sbjct: 729 CGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSL---- 788
Query: 758 LLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPT 817
D I + G TE KP
Sbjct: 789 ------------------------SDGKIA---------------LPEKKSGATEERKP- 848
Query: 818 PADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIGYHMML----SEYIDMNDSL 877
A+VLI Q QY +G ++L EY D++
Sbjct: 849 ---------AEVLIVEGQ-----------------QYAVVGTVLLLIRIILEYCQCVDNI 908
Query: 878 PALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIP 937
P+++++++ R+ ++LK+FN+R+CQLVLGAGA+QV GLK+IT+K+LAL+S+ + IP
Sbjct: 909 PSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIP 934
Query: 938 EIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEIHTKLVQIMR---ERLLVHLRGLPQ 997
IR ++P + ++L D + +DY H EI KLV IM ++LL
Sbjct: 969 VIRAHFEARLPPKQYSML-RHFDHITKDYHDHIAEISAKLVAIMDSLFDKLL-------- 934
Query: 998 IVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEA 1039
++ + P PS R++ K++ + + L E Q +F ++ + L + +
Sbjct: 1029 -----SKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQ 934
BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match:
Q5SPW0 (Vacuolar protein sorting-associated protein 54 OS=Mus musculus OX=10090 GN=Vps54 PE=1 SV=1)
HSP 1 Score: 219.9 bits (559), Expect = 1.4e-55
Identity = 268/1064 (25.19%), Postives = 444/1064 (41.73%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGK--SDASW 60
++S P + PT S L SL T + S +L + LN+P K SD
Sbjct: 33 VQSLPDVCPKEPTGDSHTLCVAPSL-VTDQHRWTVYHSKVNLPAALNDPTLAKRESDFFT 92
Query: 61 AAWWSS--SSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESV 120
W + V P +LP ++++ +F Y IS E +N +
Sbjct: 93 KTWGLDFVDTEVIPSLYLP--------QISKENFIAYQQEISQREKIHERCKNICPPKDT 152
Query: 121 GLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPF-----------LNVTQNL 180
++ + L +VP ++ K DFAL+D TF + P+ + +
Sbjct: 153 FDRTLLHIHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSK 212
Query: 181 VLQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLD 240
+LQEKLSHYLD+VE+++ +ISLRS +FF A +L + + ++ L++ I +D
Sbjct: 213 LLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQID 272
Query: 241 VDLVDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLL 300
+ + + +I TRNN + + KLKL+ V+Q +++L+++ + GALD+
Sbjct: 273 KVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQ 332
Query: 301 HLLEGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARAS 360
+L+ EL G+H FRHL + + I ++ AEF S D +
Sbjct: 333 EVLQ-QELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSD---------------LN 392
Query: 361 NLMNGKDEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVA 420
+ G+ +V EEE RL+ ++ GLL+ KL + L +Y + + K IK V
Sbjct: 393 RPLEGECQVL--EEE------RLVSLVFGLLKQRKL-NFLEIYGEEMIITAKNIIKERV- 452
Query: 421 ELLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVR 480
+ V + +D+D LA ++R L+ ++ LL IF V L R
Sbjct: 453 -INKVSQIEEIDTDVV------------VKLADQMRMLNFPQWIDLLKDIFSKFTVFLQR 512
Query: 481 AAEVKKSIEWIMCN-LDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAK 540
I ++ + L+ + +A ++ D+D L H ++
Sbjct: 513 VKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDSSLDTD---VAYLTHEGWFISDA 572
Query: 541 VTSLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHS 600
+ G+ AA + RN T P S E C S
Sbjct: 573 FSE--GEPASAAVDTTSQRN-----------TSPHS-----EPC---------SSDSVSE 632
Query: 601 VHIILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFA-ACDAAHGRW 660
D + P I ++ E+M + L N + + A D H R
Sbjct: 633 PECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRA 692
Query: 661 AKLLGVRIL--VHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDF 720
K L R KL EF+++ + + FI TE+I GR S+ G LQSQA FV+
Sbjct: 693 VKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNR 752
Query: 721 QHESRMTKIKAVLDQETWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTT 780
HE R TK+ +LD E W + DVP EFQ + +S+
Sbjct: 753 FHEERRTKLSLLLDNERWKQADVPAEFQDLVDSI-------------------------- 812
Query: 781 NNDDSSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNT 840
G + P E+ ADVL+
Sbjct: 813 ---------------------------ADGKIALPEKKPVVTEERKPADVLV-------- 872
Query: 841 NVKERGKSSSQTLQYKGIGYHMML----SEYIDMNDSLPALSSEVVHRVVEILKFFNTRT 900
+ QY +G ++L EY D++P+++++++ R+ ++LK+FN+R+
Sbjct: 873 ---------VEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRS 932
Query: 901 CQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEI 960
CQLVLGAGA+QV GLK+IT+K+LAL+S+ + IP IR ++P + ++L
Sbjct: 933 CQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSML-RHF 934
Query: 961 DRVAQDYKVHRDEIHTKLVQIMR---ERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSL 1020
D + +DY H EI KLV IM ++LL ++ + P PS R++
Sbjct: 993 DHITKDYHDHIAEISAKLVAIMDSLFDKLL-------------SKYEVKAPVPSPCFRNI 934
Query: 1021 TKEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDI 1039
K++ + + L E Q +F ++ + L + + S L++
Sbjct: 1053 CKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNV 934
BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match:
Q9VLC0 (Vacuolar protein sorting-associated protein 54 OS=Drosophila melanogaster OX=7227 GN=scat PE=1 SV=1)
HSP 1 Score: 164.1 bits (414), Expect = 9.0e-39
Identity = 224/1024 (21.88%), Postives = 383/1024 (37.40%), Query Frame = 0
Query: 37 ASSQSLSSILNNPHAGKSD----ASWAAWWSSSSTVSPPEFLPLSSTIASSEVTRFDFNN 96
+++Q+L ++LN+P GK W + S V P LP ++T DF
Sbjct: 124 SAAQNLPAVLNDPSRGKQSNLFTKKWGEHFVERSHVPPSPRLP--------DITHADFTV 183
Query: 97 YIALISDSYHRFEDIRNHSTKESVGLDSIG------GQGEALVACLREVPALYFKEDFAL 156
Y+ I Y R+ + R + L++ G G L VP ++ K L
Sbjct: 184 YLGSIGKRY-RWHERRQQQLERDKPLENGAQGAPGPGTGGQTPTHLSSVPEIFLKSQLQL 243
Query: 157 EDGATFR----------AACPFLNVTQNLVLQEKLSHYLDVVELHLVKEISLRSNSFFEA 216
ATF+ A+ P + LQE+LSHYLD+VE+ + +++S +S +FF A
Sbjct: 244 HHPATFKQVFPNYMQTSASSPESHQQTGRQLQEQLSHYLDMVEVKIAQQVSQKSAAFFHA 303
Query: 217 QGQLQDLNVKIVEGCSRIRQLKETI-RLLDVDLVDSAREIQEQNATRNNLLALQQKLKLI 276
+ ++ + ++RQL+ + L +VDS + ++ ++ L L KL+L+
Sbjct: 304 MTTQHAILAEMEQAADQVRQLRAALAELHSHSVVDSFKVLRFAQRRQHYNLTL-DKLRLM 363
Query: 277 LYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFRHLRDHVAASIESITS 336
V++ L+LL+ + D ALD+ +L EL G+HCF+HL ++ + I
Sbjct: 364 ATVHKTQPMLQLLLGTQDYVAALDLIGTTQEILSA-ELLGIHCFKHLPMQLSEMEKLIDK 423
Query: 337 ILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEEASNFRDRLLPIIIGL 396
+L+ EF R + A D++ + ++ + D V +E D+L+ I++GL
Sbjct: 424 MLTTEFER---YAAADLNRPL--------TDALRETDSVCAEE-------DKLVAIVMGL 483
Query: 397 LRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDFAPGERTVDADGGGAS 456
LR S ++ Y+ A ++ IK + E+L
Sbjct: 484 LRKQNF-SFVQAYQQEAIATIRAIIKQLLIEVL--------------------------- 543
Query: 457 LASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNLDGHYAADSVAAAIAI 516
Sbjct: 544 ------------------------------------------------------------ 603
Query: 517 GAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSNMSRNFRYCFVVLIYF 576
DSD ++ SL G A +
Sbjct: 604 ------ARSDSDQEI----------------SLTGHGEQA-----------------LEL 663
Query: 577 TFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLALSFPLSAIILVEKEEM 636
T P E A L R + + + ++ + + + A+ A+ L++ E
Sbjct: 664 TLP-------EWIALLQRSSQALVSILERIKTVVGIMQQTADAAVG-AQDAVNLIDSEAF 723
Query: 637 FERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQEFLSIYNITQDFITA 696
+ + + + A C H R A ++ + L QE + I F
Sbjct: 724 LSPGHHEQLKNQLQQLLQAVCHYCHERCANIVSPQSLERSSASEQELFQLSEIVDHFGET 783
Query: 697 TEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQAIAESL 756
T I G ++ L+ QA + H R K+ +LDQE W +VD+P EFQ I E
Sbjct: 784 TRSICGVASVPLQLALKVQASRYAQRFHSERKQKLSLLLDQERWRQVDIPHEFQRIIER- 843
Query: 757 YSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHSELTDSSDMTGGNTEH 816
M G ++
Sbjct: 844 ----------------------------------------------------MAAG--DY 897
Query: 817 AKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIGYHMMLSEYIDMNDSL 876
AKP + I N + GK TL + ML EY L
Sbjct: 904 AKPEMGNLISNG----------AGNPVLLVEGK-QPYTLVSASLMLIRMLYEYGCSAHRL 897
Query: 877 PALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIP 936
P L+S VV++L+ FN+R+CQL++GAGAM+V+GLK+ITS +LAL S+ + ++P
Sbjct: 964 PLLASYHARNVVDLLRCFNSRSCQLIIGAGAMRVAGLKTITSTNLALVSRALQLVLWLLP 897
Query: 937 EIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVE 996
+++ +S + + +DY+ H EI K+ I+ ERL L +
Sbjct: 1024 KLK----------EHFQAMSGYETIERDYQGHIKEIENKIHGIVSERLAAQL-------D 897
Query: 997 SWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSR 1040
+W + P PSQ R +++ + L ++ L EA + I+ V + F ++ E +
Sbjct: 1084 AW---EARPPIPSQTFRHISRHLVKLHEAIAGVLPEAQIHEIYGVVHRNFKDKLREQLLK 897
BLAST of MC01g1658 vs. NCBI nr
Match:
XP_022146560.1 (vacuolar protein sorting-associated protein 54, chloroplastic [Momordica charantia])
HSP 1 Score: 1898 bits (4917), Expect = 0.0
Identity = 1014/1099 (92.27%), Postives = 1014/1099 (92.27%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA
Sbjct: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
Query: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNY ALISDSYHRFEDIRNHSTKESVGLDS
Sbjct: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYTALISDSYHRFEDIRNHSTKESVGLDS 120
Query: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL
Sbjct: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
Query: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
Query: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR
Sbjct: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
Query: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE
Sbjct: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
Query: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF
Sbjct: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
Query: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL
Sbjct: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
Query: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN
Sbjct: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
Query: 541 MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
MSRNFR
Sbjct: 541 MSRNFR------------------------------------------------------ 600
Query: 601 SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601 ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
Query: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV
Sbjct: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
Query: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS
Sbjct: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
Query: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG
Sbjct: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
Query: 841 YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
YHM MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841 YHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
Query: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI
Sbjct: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
Query: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT
Sbjct: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1024
Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1024
BLAST of MC01g1658 vs. NCBI nr
Match:
XP_038882115.1 (vacuolar protein sorting-associated protein 54, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 1769 bits (4581), Expect = 0.0
Identity = 946/1102 (85.84%), Postives = 979/1102 (88.84%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGKSDAS 60
MESQPSQSGRSPT+YSTL+SR+TSL RTTSS K+NSDASSQSLSSILNNPHAGKSDAS
Sbjct: 1 MESQPSQSGRSPTDYSTLVSRQTSLGRTTSSSSAKSNSDASSQSLSSILNNPHAGKSDAS 60
Query: 61 WAAWWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVG 120
W WWSSSST+SPPEF+PL+STIA+SEVTRFDFNNY ALISDS++RFEDIRNHS+KE+ G
Sbjct: 61 WVGWWSSSSTISPPEFMPLASTIATSEVTRFDFNNYTALISDSFNRFEDIRNHSSKENGG 120
Query: 121 LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDV 180
LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNV+QNLVLQEKLSHYLDV
Sbjct: 121 LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNLVLQEKLSHYLDV 180
Query: 181 VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQE 240
VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLD DLVDSAREIQE
Sbjct: 181 VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDADLVDSAREIQE 240
Query: 241 QNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLH 300
QNATRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLH
Sbjct: 241 QNATRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300
Query: 301 CFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFD 360
CFRHLRDHVAASIESITSILSAEFMRAS HDAGDVD+ II ETKA ASNLMNGKDEVK D
Sbjct: 301 CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIGIITETKAWASNLMNGKDEVKLD 360
Query: 361 EEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLD 420
EEE SNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLL+RPLD
Sbjct: 361 EEETSNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLIRPLD 420
Query: 421 SDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIM 480
SDFAPGERT+DADGGG SLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIM
Sbjct: 421 SDFAPGERTMDADGGGVSLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIM 480
Query: 481 CNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAAN 540
CNLDGHYAADSVAAAIA GAAAAGTAQD+DNQ GLLLPHLPQRVAAKVTSL GKANDAAN
Sbjct: 481 CNLDGHYAADSVAAAIATGAAAAGTAQDTDNQGGLLLPHLPQRVAAKVTSLQGKANDAAN 540
Query: 541 PSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLAST 600
PSNMSRNFR
Sbjct: 541 PSNMSRNFR--------------------------------------------------- 600
Query: 601 LALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKL 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKL
Sbjct: 601 ------------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKL 660
Query: 661 RLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQE 720
RLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQE
Sbjct: 661 RLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQE 720
Query: 721 TWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQ 780
TWVEVDVPDEFQ+IAESL SQELLS K+D AQGNMD+SY+DV TNND S IVGGGSLNAQ
Sbjct: 721 TWVEVDVPDEFQSIAESLCSQELLSEKVDLAQGNMDQSYSDVATNNDASRIVGGGSLNAQ 780
Query: 781 QHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYK 840
+SE DSSDM+GGNTEH KPTPAD IEKS+ADV IPT QINN NVKERGKSSSQTL +K
Sbjct: 781 HYSEQIDSSDMSGGNTEHVKPTPADTIEKSRADVTIPTMQINNINVKERGKSSSQTLLHK 840
Query: 841 GIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQ 900
G+GYHM MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQ
Sbjct: 841 GVGYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQ 900
Query: 901 VSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHR 960
VSGLKSITSKHLALASQVISFT AIIPEIRRILF KVPE RKTLL+SEIDRVAQD+KVHR
Sbjct: 901 VSGLKSITSKHLALASQVISFTFAIIPEIRRILFFKVPEVRKTLLLSEIDRVAQDFKVHR 960
Query: 961 DEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRT 1020
DEIHTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRT
Sbjct: 961 DEIHTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRT 1020
Query: 1021 LHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK 1080
LHEADV+AIF++VVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK
Sbjct: 1021 LHEADVKAIFREVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK 1027
Query: 1081 PDTPNWGQLDEFLDQRFGSEAG 1090
PD PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PDIPNWGQLDEFLDQRFGSEAG 1027
BLAST of MC01g1658 vs. NCBI nr
Match:
XP_004134530.1 (vacuolar protein sorting-associated protein 54, chloroplastic [Cucumis sativus] >KGN49522.1 hypothetical protein Csa_003061 [Cucumis sativus])
HSP 1 Score: 1744 bits (4517), Expect = 0.0
Identity = 936/1099 (85.17%), Postives = 970/1099 (88.26%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
M+SQPSQSGRSPT+YST+++R+TSL RTTSS SDASSQSLSSILNNPHAGKSDASW
Sbjct: 1 MDSQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60
Query: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
WWSSSSTV+PPEF+PLSSTIASSEVTRFDFNNY ALISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61 WWSSSSTVNPPEFMPLSSTIASSEVTRFDFNNYTALISDSFHRFEDIRNHSSKENGGLDS 120
Query: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121 IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180
Query: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
Query: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300
Query: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301 HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360
Query: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLL+RP DSDF
Sbjct: 361 TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLIRPHDSDF 420
Query: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHLVRAAEVKKSIEWIMCNL
Sbjct: 421 APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLVRAAEVKKSIEWIMCNL 480
Query: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
DGHYAADSVAAAIA GAAAAGTAQD+DNQ GLLLPHLPQRVAAKV SL GKANDAANPSN
Sbjct: 481 DGHYAADSVAAAIASGAAAAGTAQDTDNQGGLLLPHLPQRVAAKVISLQGKANDAANPSN 540
Query: 541 MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
MSRNFR
Sbjct: 541 MSRNFR------------------------------------------------------ 600
Query: 601 SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601 ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
Query: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVD+QHESRMTKIKAVLDQETWV
Sbjct: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDYQHESRMTKIKAVLDQETWV 720
Query: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
EVDVPDEFQ+IAESL SQELLS K D Q NMDRSY DV TNNDDS NAQQHS
Sbjct: 721 EVDVPDEFQSIAESLCSQELLSEKPDLTQDNMDRSYGDVATNNDDSH-------NAQQHS 780
Query: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
E DSSD++GGN+EH KPTPAD EKSKADV IPT Q++NTNVKERGKSSSQTL YKG+G
Sbjct: 781 EQIDSSDLSGGNSEHVKPTPADTTEKSKADVTIPTMQVSNTNVKERGKSSSQTLLYKGVG 840
Query: 841 YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
YHM MLSEYIDMN+SLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841 YHMVNCGLILLKMLSEYIDMNNSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
Query: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901 LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960
Query: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014
Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPD
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDI 1014
Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
PNWGQLDEFL+QRFGSEAG
Sbjct: 1081 PNWGQLDEFLEQRFGSEAG 1014
BLAST of MC01g1658 vs. NCBI nr
Match:
KAA0052432.1 (vacuolar protein sorting-associated protein 54 [Cucumis melo var. makuwa])
HSP 1 Score: 1735 bits (4493), Expect = 0.0
Identity = 931/1099 (84.71%), Postives = 971/1099 (88.35%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
MESQPSQSGRSPT+YST+++R+TSL RTTSS SDASSQSLSSILNNPHAGKSDASW
Sbjct: 1 MESQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60
Query: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
WWSSSSTV+PPEF+PLSS+IASSEVTRFDFNNY LISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61 WWSSSSTVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDS 120
Query: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121 IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180
Query: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
Query: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300
Query: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301 HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360
Query: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELL VLL+RPLDSDF
Sbjct: 361 TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLHVLLIRPLDSDF 420
Query: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHL+RAAEVKKSIEWIMCNL
Sbjct: 421 APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLMRAAEVKKSIEWIMCNL 480
Query: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
DGHYAADSVAAAIA GAAAAGTAQD+D+Q GLLLPHLPQRVAAKVTSL GKANDAANPSN
Sbjct: 481 DGHYAADSVAAAIASGAAAAGTAQDTDSQGGLLLPHLPQRVAAKVTSLQGKANDAANPSN 540
Query: 541 MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
MSRNFR
Sbjct: 541 MSRNFR------------------------------------------------------ 600
Query: 601 SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601 ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
Query: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWV
Sbjct: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWV 720
Query: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
EVDVPDEFQ+IAESL SQELLS D AQGNMD+S++DV T+NDDS NAQ+HS
Sbjct: 721 EVDVPDEFQSIAESLCSQELLSENPDLAQGNMDQSHSDVATDNDDS-------RNAQEHS 780
Query: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
+ DSSD++GGN+EH K TPAD IEKSKADV IP+TQ+NNTNVKERGKSSSQTL YKG+G
Sbjct: 781 QQIDSSDLSGGNSEHVKLTPADTIEKSKADVTIPSTQLNNTNVKERGKSSSQTLLYKGVG 840
Query: 841 YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
YHM MLSEYIDMN+SLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841 YHMVNCGLILLKMLSEYIDMNNSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
Query: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901 LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960
Query: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014
Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK D
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKSDI 1014
Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1014
BLAST of MC01g1658 vs. NCBI nr
Match:
XP_008439467.1 (PREDICTED: vacuolar protein sorting-associated protein 54, chloroplastic [Cucumis melo])
HSP 1 Score: 1733 bits (4489), Expect = 0.0
Identity = 930/1099 (84.62%), Postives = 970/1099 (88.26%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
MESQPSQSGRSPT+YST+++R+TSL RTTSS SDASSQSLSSILNNPHAGKSDASW
Sbjct: 1 MESQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60
Query: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
WWSSSSTV+PPEF+PLSS+IASSEVTRFDFNNY LISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61 WWSSSSTVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDS 120
Query: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121 IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180
Query: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
Query: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300
Query: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301 HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360
Query: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELL VLL+RPLDSDF
Sbjct: 361 TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLHVLLIRPLDSDF 420
Query: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHL+RAAEVKKSIEWIMCNL
Sbjct: 421 APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLMRAAEVKKSIEWIMCNL 480
Query: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
DGHYAADSVAAAIA GAAAAGTAQD+D+Q GLLLPHLPQRVAAKVTSL GKANDAANPSN
Sbjct: 481 DGHYAADSVAAAIASGAAAAGTAQDTDSQGGLLLPHLPQRVAAKVTSLQGKANDAANPSN 540
Query: 541 MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
MSRNFR
Sbjct: 541 MSRNFR------------------------------------------------------ 600
Query: 601 SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601 ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
Query: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWV
Sbjct: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWV 720
Query: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
EVDVPDEFQ+IAESL SQELLS D AQGNMD+S++DV T+NDDS NAQ+HS
Sbjct: 721 EVDVPDEFQSIAESLCSQELLSENPDLAQGNMDQSHSDVATDNDDS-------RNAQEHS 780
Query: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
+ DSSD++GGN+EH K TPAD IEKSKADV IP+TQ+NNTNVKERGKSSSQTL YKG+G
Sbjct: 781 QQIDSSDLSGGNSEHVKLTPADTIEKSKADVTIPSTQLNNTNVKERGKSSSQTLLYKGVG 840
Query: 841 YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
YHM MLSEYIDMN+S PALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841 YHMVNCGLILLKMLSEYIDMNNSFPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
Query: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901 LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960
Query: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014
Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK D
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKSDI 1014
Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1014
BLAST of MC01g1658 vs. ExPASy TrEMBL
Match:
A0A6J1CXK3 (vacuolar protein sorting-associated protein 54, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015746 PE=3 SV=1)
HSP 1 Score: 1898 bits (4917), Expect = 0.0
Identity = 1014/1099 (92.27%), Postives = 1014/1099 (92.27%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA
Sbjct: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
Query: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNY ALISDSYHRFEDIRNHSTKESVGLDS
Sbjct: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYTALISDSYHRFEDIRNHSTKESVGLDS 120
Query: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL
Sbjct: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
Query: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
Query: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR
Sbjct: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
Query: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE
Sbjct: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
Query: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF
Sbjct: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
Query: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL
Sbjct: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
Query: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN
Sbjct: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
Query: 541 MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
MSRNFR
Sbjct: 541 MSRNFR------------------------------------------------------ 600
Query: 601 SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601 ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
Query: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV
Sbjct: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
Query: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS
Sbjct: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
Query: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG
Sbjct: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
Query: 841 YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
YHM MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841 YHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
Query: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI
Sbjct: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
Query: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT
Sbjct: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1024
Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1024
BLAST of MC01g1658 vs. ExPASy TrEMBL
Match:
A0A0A0KMC9 (Vps54 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G526560 PE=3 SV=1)
HSP 1 Score: 1744 bits (4517), Expect = 0.0
Identity = 936/1099 (85.17%), Postives = 970/1099 (88.26%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
M+SQPSQSGRSPT+YST+++R+TSL RTTSS SDASSQSLSSILNNPHAGKSDASW
Sbjct: 1 MDSQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60
Query: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
WWSSSSTV+PPEF+PLSSTIASSEVTRFDFNNY ALISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61 WWSSSSTVNPPEFMPLSSTIASSEVTRFDFNNYTALISDSFHRFEDIRNHSSKENGGLDS 120
Query: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121 IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180
Query: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
Query: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300
Query: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301 HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360
Query: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLL+RP DSDF
Sbjct: 361 TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLIRPHDSDF 420
Query: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHLVRAAEVKKSIEWIMCNL
Sbjct: 421 APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLVRAAEVKKSIEWIMCNL 480
Query: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
DGHYAADSVAAAIA GAAAAGTAQD+DNQ GLLLPHLPQRVAAKV SL GKANDAANPSN
Sbjct: 481 DGHYAADSVAAAIASGAAAAGTAQDTDNQGGLLLPHLPQRVAAKVISLQGKANDAANPSN 540
Query: 541 MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
MSRNFR
Sbjct: 541 MSRNFR------------------------------------------------------ 600
Query: 601 SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601 ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
Query: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVD+QHESRMTKIKAVLDQETWV
Sbjct: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDYQHESRMTKIKAVLDQETWV 720
Query: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
EVDVPDEFQ+IAESL SQELLS K D Q NMDRSY DV TNNDDS NAQQHS
Sbjct: 721 EVDVPDEFQSIAESLCSQELLSEKPDLTQDNMDRSYGDVATNNDDSH-------NAQQHS 780
Query: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
E DSSD++GGN+EH KPTPAD EKSKADV IPT Q++NTNVKERGKSSSQTL YKG+G
Sbjct: 781 EQIDSSDLSGGNSEHVKPTPADTTEKSKADVTIPTMQVSNTNVKERGKSSSQTLLYKGVG 840
Query: 841 YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
YHM MLSEYIDMN+SLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841 YHMVNCGLILLKMLSEYIDMNNSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
Query: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901 LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960
Query: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014
Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPD
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDI 1014
Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
PNWGQLDEFL+QRFGSEAG
Sbjct: 1081 PNWGQLDEFLEQRFGSEAG 1014
BLAST of MC01g1658 vs. ExPASy TrEMBL
Match:
A0A5A7U9K3 (Vacuolar protein sorting-associated protein 54 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00290 PE=3 SV=1)
HSP 1 Score: 1735 bits (4493), Expect = 0.0
Identity = 931/1099 (84.71%), Postives = 971/1099 (88.35%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
MESQPSQSGRSPT+YST+++R+TSL RTTSS SDASSQSLSSILNNPHAGKSDASW
Sbjct: 1 MESQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60
Query: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
WWSSSSTV+PPEF+PLSS+IASSEVTRFDFNNY LISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61 WWSSSSTVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDS 120
Query: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121 IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180
Query: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
Query: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300
Query: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301 HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360
Query: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELL VLL+RPLDSDF
Sbjct: 361 TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLHVLLIRPLDSDF 420
Query: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHL+RAAEVKKSIEWIMCNL
Sbjct: 421 APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLMRAAEVKKSIEWIMCNL 480
Query: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
DGHYAADSVAAAIA GAAAAGTAQD+D+Q GLLLPHLPQRVAAKVTSL GKANDAANPSN
Sbjct: 481 DGHYAADSVAAAIASGAAAAGTAQDTDSQGGLLLPHLPQRVAAKVTSLQGKANDAANPSN 540
Query: 541 MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
MSRNFR
Sbjct: 541 MSRNFR------------------------------------------------------ 600
Query: 601 SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601 ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
Query: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWV
Sbjct: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWV 720
Query: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
EVDVPDEFQ+IAESL SQELLS D AQGNMD+S++DV T+NDDS NAQ+HS
Sbjct: 721 EVDVPDEFQSIAESLCSQELLSENPDLAQGNMDQSHSDVATDNDDS-------RNAQEHS 780
Query: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
+ DSSD++GGN+EH K TPAD IEKSKADV IP+TQ+NNTNVKERGKSSSQTL YKG+G
Sbjct: 781 QQIDSSDLSGGNSEHVKLTPADTIEKSKADVTIPSTQLNNTNVKERGKSSSQTLLYKGVG 840
Query: 841 YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
YHM MLSEYIDMN+SLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841 YHMVNCGLILLKMLSEYIDMNNSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
Query: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901 LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960
Query: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014
Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK D
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKSDI 1014
Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1014
BLAST of MC01g1658 vs. ExPASy TrEMBL
Match:
A0A1S3AYS9 (vacuolar protein sorting-associated protein 54, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484260 PE=3 SV=1)
HSP 1 Score: 1733 bits (4489), Expect = 0.0
Identity = 930/1099 (84.62%), Postives = 970/1099 (88.26%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
MESQPSQSGRSPT+YST+++R+TSL RTTSS SDASSQSLSSILNNPHAGKSDASW
Sbjct: 1 MESQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60
Query: 61 WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
WWSSSSTV+PPEF+PLSS+IASSEVTRFDFNNY LISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61 WWSSSSTVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDS 120
Query: 121 IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121 IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180
Query: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181 HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
Query: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241 TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300
Query: 301 HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301 HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360
Query: 361 ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELL VLL+RPLDSDF
Sbjct: 361 TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLHVLLIRPLDSDF 420
Query: 421 APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHL+RAAEVKKSIEWIMCNL
Sbjct: 421 APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLMRAAEVKKSIEWIMCNL 480
Query: 481 DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
DGHYAADSVAAAIA GAAAAGTAQD+D+Q GLLLPHLPQRVAAKVTSL GKANDAANPSN
Sbjct: 481 DGHYAADSVAAAIASGAAAAGTAQDTDSQGGLLLPHLPQRVAAKVTSLQGKANDAANPSN 540
Query: 541 MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
MSRNFR
Sbjct: 541 MSRNFR------------------------------------------------------ 600
Query: 601 SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601 ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
Query: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWV
Sbjct: 661 EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWV 720
Query: 721 EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
EVDVPDEFQ+IAESL SQELLS D AQGNMD+S++DV T+NDDS NAQ+HS
Sbjct: 721 EVDVPDEFQSIAESLCSQELLSENPDLAQGNMDQSHSDVATDNDDS-------RNAQEHS 780
Query: 781 ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
+ DSSD++GGN+EH K TPAD IEKSKADV IP+TQ+NNTNVKERGKSSSQTL YKG+G
Sbjct: 781 QQIDSSDLSGGNSEHVKLTPADTIEKSKADVTIPSTQLNNTNVKERGKSSSQTLLYKGVG 840
Query: 841 YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
YHM MLSEYIDMN+S PALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841 YHMVNCGLILLKMLSEYIDMNNSFPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
Query: 901 LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901 LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960
Query: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961 HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014
Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK D
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKSDI 1014
Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1014
BLAST of MC01g1658 vs. ExPASy TrEMBL
Match:
A0A6J1EEU2 (vacuolar protein sorting-associated protein 54, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433683 PE=3 SV=1)
HSP 1 Score: 1722 bits (4459), Expect = 0.0
Identity = 928/1106 (83.91%), Postives = 967/1106 (87.43%), Query Frame = 0
Query: 1 MESQPSQSGRSPTEYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGKSDAS 60
M+SQPSQSGRSPTEYS+LLSRET+L RTTSS K+N DASSQSLSSILNNPHAGKSDAS
Sbjct: 1 MDSQPSQSGRSPTEYSSLLSRETTLGRTTSSSSPKSNPDASSQSLSSILNNPHAGKSDAS 60
Query: 61 WAAWWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVG 120
W WWSSSSTV+PPEF+PL+S+ ASSEVTRFDFNNY ALISDSY+RFEDIRNHS+KE+ G
Sbjct: 61 WVGWWSSSSTVTPPEFIPLTSSTASSEVTRFDFNNYTALISDSYNRFEDIRNHSSKENGG 120
Query: 121 LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDV 180
LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNV+QNLVLQEKLSHYLDV
Sbjct: 121 LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNLVLQEKLSHYLDV 180
Query: 181 VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQE 240
VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSR+RQLKETIRLLDVDLVDSAR+IQE
Sbjct: 181 VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRLRQLKETIRLLDVDLVDSARQIQE 240
Query: 241 QNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLH 300
QNATRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLH
Sbjct: 241 QNATRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300
Query: 301 CFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKF- 360
CFRHLRDHVAAS+ESITSILSAEFMRAS HDAGDVDLVII ETKA SNLMNGKDEVK
Sbjct: 301 CFRHLRDHVAASLESITSILSAEFMRASIHDAGDVDLVIISETKASISNLMNGKDEVKLT 360
Query: 361 ---DEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLM 420
DEEE SNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVT DMKTAIKNAVAELLPVLL+
Sbjct: 361 CILDEEETSNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTVDMKTAIKNAVAELLPVLLV 420
Query: 421 RPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSI 480
RPLDSDFAPGERT D DGG ASLASKLRGLSSEGFVQLL+AIFKIVQVHLVRAAEVKKSI
Sbjct: 421 RPLDSDFAPGERTKDTDGG-ASLASKLRGLSSEGFVQLLNAIFKIVQVHLVRAAEVKKSI 480
Query: 481 EWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKAN 540
EWIMCNLDGHYAADSVAAAIA GAAA+GTAQDSDNQ GL LPH+PQ AAKVTSL GKAN
Sbjct: 481 EWIMCNLDGHYAADSVAAAIATGAAASGTAQDSDNQGGLPLPHVPQG-AAKVTSLQGKAN 540
Query: 541 DAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQK 600
DAANPSNMSRNFR
Sbjct: 541 DAANPSNMSRNFR----------------------------------------------- 600
Query: 601 LASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILV 660
ADVLRENTEAVFAACDAAHGRWAKLLGVRILV
Sbjct: 601 ----------------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILV 660
Query: 661 HPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAV 720
HPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHE RMTKIKAV
Sbjct: 661 HPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHEFRMTKIKAV 720
Query: 721 LDQETWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGS 780
LD ETWVEVDVPDEFQ IAESL ELLSAK+DD+QGNMD+SY+DV TNN+D+ I+GG
Sbjct: 721 LDVETWVEVDVPDEFQTIAESLCFHELLSAKLDDSQGNMDQSYSDVATNNEDARIIGG-- 780
Query: 781 LNAQQHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQT 840
+NAQQHSE DSSD++G NTEH KPTPAD IE SKADV IP TQ NNTNVKERGKSSS T
Sbjct: 781 VNAQQHSEQVDSSDISGRNTEHVKPTPADTIENSKADVAIPATQNNNTNVKERGKSSSLT 840
Query: 841 LQYKGIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGA 900
LQYKG+GYHM MLSEY+DMNDSLPALSSE+VHRVVEILKFFNTRTCQLVLGA
Sbjct: 841 LQYKGVGYHMVNCGLILLKMLSEYVDMNDSLPALSSEIVHRVVEILKFFNTRTCQLVLGA 900
Query: 901 GAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDY 960
GAMQVSGLKSITSKHLALASQVISFT AIIPE+RRILFLKVPEARKTLL+SEIDRVAQDY
Sbjct: 901 GAMQVSGLKSITSKHLALASQVISFTFAIIPEMRRILFLKVPEARKTLLLSEIDRVAQDY 960
Query: 961 KVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRV 1020
KVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRL+D+DPQPSQFARSLTKEVGYLQRV
Sbjct: 961 KVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLEDADPQPSQFARSLTKEVGYLQRV 1020
Query: 1021 LSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCD 1080
LSRTLHEADV+AIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCD
Sbjct: 1021 LSRTLHEADVKAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCD 1027
Query: 1081 DLSKPDTPNWGQLDEFLDQRFGSEAG 1090
D SKPD PNWGQLDEFLDQR GSEAG
Sbjct: 1081 DSSKPDIPNWGQLDEFLDQRLGSEAG 1027
BLAST of MC01g1658 vs. TAIR 10
Match:
AT4G19490.1 (VPS54 )
HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 670/1113 (60.20%), Postives = 807/1113 (72.51%), Query Frame = 0
Query: 1 MESQPSQSGRSPT---EYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGK- 60
M+S PS GRS T S L R +S ++ S K+ SDASSQSLSSILNNPH GK
Sbjct: 1 MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60
Query: 61 ----SDASWAAWWSSSSTVSPPEFLPLSST-IASSEVTRFDFNNYIALISDSYHRFEDIR 120
SDASW WWSSS+ V+P EF P++ST + SE+TR DF+ Y++ IS+S+ RFEDIR
Sbjct: 61 GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120
Query: 121 NHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQ 180
H+ +ES G D L ACLREVP+LYFKEDFALEDGATFR+ACPF ++ +NL LQ
Sbjct: 121 KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180
Query: 181 EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDL 240
EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR+LKETIRLLD +L
Sbjct: 181 EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240
Query: 241 VDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLL 300
VDSAR+IQE ++TR N+L LQ+KL+LILYVNQA+SALKLLVAS DCAGALD+TDDL +LL
Sbjct: 241 VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300
Query: 301 EGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLM 360
GDEL+GL+CFRHLRDHV +SI+SI SIL++EFMR S HD G++D++I+ R S
Sbjct: 301 AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360
Query: 361 NGK--DEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAE 420
NG +EVK +EE+ S DRLLP++IGLLRTAK PS+LR+YRD +T++MK AIK AVA+
Sbjct: 361 NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420
Query: 421 LLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRA 480
LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AIFKIVQ HLVRA
Sbjct: 421 LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480
Query: 481 AEVKKSIEWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVT 540
+EVKK+IEWI+CN+DGHYAADSVAAAIA+GA AA TAQ+ Q G L+ + +K
Sbjct: 481 SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540
Query: 541 SLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVH 600
L GK++DA++ NMSRNFR
Sbjct: 541 PLQGKSSDASSLMNMSRNFR---------------------------------------- 600
Query: 601 IILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKL 660
ADVLRENTEAVFAAC+ HGRWAKL
Sbjct: 601 -----------------------------------ADVLRENTEAVFAACEVTHGRWAKL 660
Query: 661 LGVRILVHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESR 720
LGVR L+HPKL+LQEF+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESR
Sbjct: 661 LGVRALLHPKLKLQEFMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESR 720
Query: 721 MTKIKAVLDQETWVEVDVPDEFQAIAESLY-SQELLSAKIDDAQGNMDRSYTDVTTNNDD 780
MTK+KAVLDQETW E+DVP+EFQ+I SL+ SQ L+S K+DDA N SY +
Sbjct: 721 MTKLKAVLDQETWDEIDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNG 780
Query: 781 SSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPA-DKIEKSKADVLIPTTQINNTNVK 840
S G G N++ +E ++SS+ + + KPT + + +E+SKA V T N +N K
Sbjct: 781 SLTSGSGDQNSELRNEKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQK 840
Query: 841 ERGKSSSQTLQYKGIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNT 900
GKS+ L Y+G+GYHM MLSEYIDMN+SLPALSSE+V RVVE+L+FFNT
Sbjct: 841 AHGKSN---LFYQGVGYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNT 900
Query: 901 RTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLIS 960
RTCQLVLGAGAMQVSGLKSI +KHLALASQVI FT IIPE RRILF KVPE RK LL
Sbjct: 901 RTCQLVLGAGAMQVSGLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSV 960
Query: 961 EIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLT 1020
EID+VAQD+++HRDEI+TKLVQIMRERLL HL GLP++VE WNR D++ Q +FA LT
Sbjct: 961 EIDKVAQDFRIHRDEIYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLT 1020
Query: 1021 KEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHIL 1080
+EVGYL RVLS TLHEADVQAIF+QV+ I H Q S+ + L+IS+ +AK RL V+ IL
Sbjct: 1021 REVGYLHRVLSETLHEADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELIL 1028
Query: 1081 GCIRSLPCDDLSKPDTPNWGQLDEFLDQRFGSE 1089
CIRSLP D+ ++ D PNWGQLDEF + F E
Sbjct: 1081 KCIRSLPSDNANQSDIPNWGQLDEFFAEHFREE 1028
BLAST of MC01g1658 vs. TAIR 10
Match:
AT4G19490.2 (VPS54 )
HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 670/1113 (60.20%), Postives = 807/1113 (72.51%), Query Frame = 0
Query: 1 MESQPSQSGRSPT---EYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGK- 60
M+S PS GRS T S L R +S ++ S K+ SDASSQSLSSILNNPH GK
Sbjct: 1 MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60
Query: 61 ----SDASWAAWWSSSSTVSPPEFLPLSST-IASSEVTRFDFNNYIALISDSYHRFEDIR 120
SDASW WWSSS+ V+P EF P++ST + SE+TR DF+ Y++ IS+S+ RFEDIR
Sbjct: 61 GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120
Query: 121 NHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQ 180
H+ +ES G D L ACLREVP+LYFKEDFALEDGATFR+ACPF ++ +NL LQ
Sbjct: 121 KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180
Query: 181 EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDL 240
EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR+LKETIRLLD +L
Sbjct: 181 EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240
Query: 241 VDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLL 300
VDSAR+IQE ++TR N+L LQ+KL+LILYVNQA+SALKLLVAS DCAGALD+TDDL +LL
Sbjct: 241 VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300
Query: 301 EGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLM 360
GDEL+GL+CFRHLRDHV +SI+SI SIL++EFMR S HD G++D++I+ R S
Sbjct: 301 AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360
Query: 361 NGK--DEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAE 420
NG +EVK +EE+ S DRLLP++IGLLRTAK PS+LR+YRD +T++MK AIK AVA+
Sbjct: 361 NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420
Query: 421 LLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRA 480
LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AIFKIVQ HLVRA
Sbjct: 421 LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480
Query: 481 AEVKKSIEWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVT 540
+EVKK+IEWI+CN+DGHYAADSVAAAIA+GA AA TAQ+ Q G L+ + +K
Sbjct: 481 SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540
Query: 541 SLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVH 600
L GK++DA++ NMSRNFR
Sbjct: 541 PLQGKSSDASSLMNMSRNFR---------------------------------------- 600
Query: 601 IILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKL 660
ADVLRENTEAVFAAC+ HGRWAKL
Sbjct: 601 -----------------------------------ADVLRENTEAVFAACEVTHGRWAKL 660
Query: 661 LGVRILVHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESR 720
LGVR L+HPKL+LQEF+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESR
Sbjct: 661 LGVRALLHPKLKLQEFMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESR 720
Query: 721 MTKIKAVLDQETWVEVDVPDEFQAIAESLY-SQELLSAKIDDAQGNMDRSYTDVTTNNDD 780
MTK+KAVLDQETW E+DVP+EFQ+I SL+ SQ L+S K+DDA N SY +
Sbjct: 721 MTKLKAVLDQETWDEIDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNG 780
Query: 781 SSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPA-DKIEKSKADVLIPTTQINNTNVK 840
S G G N++ +E ++SS+ + + KPT + + +E+SKA V T N +N K
Sbjct: 781 SLTSGSGDQNSELRNEKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQK 840
Query: 841 ERGKSSSQTLQYKGIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNT 900
GKS+ L Y+G+GYHM MLSEYIDMN+SLPALSSE+V RVVE+L+FFNT
Sbjct: 841 AHGKSN---LFYQGVGYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNT 900
Query: 901 RTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLIS 960
RTCQLVLGAGAMQVSGLKSI +KHLALASQVI FT IIPE RRILF KVPE RK LL
Sbjct: 901 RTCQLVLGAGAMQVSGLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSV 960
Query: 961 EIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLT 1020
EID+VAQD+++HRDEI+TKLVQIMRERLL HL GLP++VE WNR D++ Q +FA LT
Sbjct: 961 EIDKVAQDFRIHRDEIYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLT 1020
Query: 1021 KEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHIL 1080
+EVGYL RVLS TLHEADVQAIF+QV+ I H Q S+ + L+IS+ +AK RL V+ IL
Sbjct: 1021 REVGYLHRVLSETLHEADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELIL 1028
Query: 1081 GCIRSLPCDDLSKPDTPNWGQLDEFLDQRFGSE 1089
CIRSLP D+ ++ D PNWGQLDEF + F E
Sbjct: 1081 KCIRSLPSDNANQSDIPNWGQLDEFFAEHFREE 1028
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F4JT76 | 0.0e+00 | 60.20 | Vacuolar protein sorting-associated protein 54, chloroplastic OS=Arabidopsis tha... | [more] |
Q9JMK8 | 3.0e-58 | 25.73 | Vacuolar protein sorting-associated protein 54 OS=Rattus norvegicus OX=10116 GN=... | [more] |
Q9P1Q0 | 5.1e-58 | 25.34 | Vacuolar protein sorting-associated protein 54 OS=Homo sapiens OX=9606 GN=VPS54 ... | [more] |
Q5SPW0 | 1.4e-55 | 25.19 | Vacuolar protein sorting-associated protein 54 OS=Mus musculus OX=10090 GN=Vps54... | [more] |
Q9VLC0 | 9.0e-39 | 21.88 | Vacuolar protein sorting-associated protein 54 OS=Drosophila melanogaster OX=722... | [more] |
Match Name | E-value | Identity | Description | |
XP_022146560.1 | 0.0 | 92.27 | vacuolar protein sorting-associated protein 54, chloroplastic [Momordica charant... | [more] |
XP_038882115.1 | 0.0 | 85.84 | vacuolar protein sorting-associated protein 54, chloroplastic isoform X1 [Beninc... | [more] |
XP_004134530.1 | 0.0 | 85.17 | vacuolar protein sorting-associated protein 54, chloroplastic [Cucumis sativus] ... | [more] |
KAA0052432.1 | 0.0 | 84.71 | vacuolar protein sorting-associated protein 54 [Cucumis melo var. makuwa] | [more] |
XP_008439467.1 | 0.0 | 84.62 | PREDICTED: vacuolar protein sorting-associated protein 54, chloroplastic [Cucumi... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CXK3 | 0.0 | 92.27 | vacuolar protein sorting-associated protein 54, chloroplastic OS=Momordica chara... | [more] |
A0A0A0KMC9 | 0.0 | 85.17 | Vps54 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G526560 PE=3 ... | [more] |
A0A5A7U9K3 | 0.0 | 84.71 | Vacuolar protein sorting-associated protein 54 OS=Cucumis melo var. makuwa OX=11... | [more] |
A0A1S3AYS9 | 0.0 | 84.62 | vacuolar protein sorting-associated protein 54, chloroplastic OS=Cucumis melo OX... | [more] |
A0A6J1EEU2 | 0.0 | 83.91 | vacuolar protein sorting-associated protein 54, chloroplastic-like OS=Cucurbita ... | [more] |