MC01g1658 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g1658
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionvacuolar protein sorting-associated protein 54, chloroplastic
LocationMC01: 20723827 .. 20739831 (-)
RNA-Seq ExpressionMC01g1658
SyntenyMC01g1658
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTTACAATTATATTATAATATTACAAGGTAATGTGTAATTGCGTAATGAAGGCCAAGGCCAAGGGTGTGGAATCGGCAGGTGGAGGTGGGCAGTGATGTGGTATAGGAATGGATTCCATTTCATGAGCCCAGAAATTTTGAACGAGATCTACCACCGGAGTTGCATTGGAAGGAGTTCGTTTTTCTTTCCTTCATCCAGCGCCAACTTCCTTCTCTTTTCGTGGCTTTTCTTCGTCGTCTGTTGAGATCTGGTCTGAACCACTTCATCTCGATCTCTATCGCTTTGGAATTCGACTACTGAACGTGATATTCTGTCTGGAACCCGCCATTCATTCATATGATTCTCAGATTTTGAGCATGGAGTCCCAGCCTTCCCAATCGGGAAGGTCCCCTACTGAATACTCCACCCTTCTCAGTAGAGAAACGAGTCTTCGTCGAACTACTTCCTCTAAAACCAACTCCGATGCCAGCAGCCAGAGCTTGTCTTCAATTCTCAACAACCCGCACGCTGGCAAATCCGATGCCTCTTGGGCTGCCTGGTGGTCGTCTTCGTCCACCGTCAGCCCGCCTGAGTTCTTGCCCCTCTCCTCCACCATAGCCTCCTCTGAAGTTACTCGATTCGATTTCAACAACTACATCGCCTTGATCTCCGATTCTTATCATCGATTCGAGGACATACGGAACCATTCCACCAAAGAGAGCGTCGGCTTGGATAGCATTGGGGGCCAGGGGGAAGCGCTTGTGGCATGTTTGAGAGAGGTTCCCGCGCTTTACTTCAAGGAAGATTTCGCATTGGAGGACGGGGCGACATTCCGTGCAGCCTGTCCCTTCTTGAATGTAACGCAGAATTTGGTGCTCCAAGAGAAGCTTTCACATTATTTAGATGTCGTGGAGTTGCATTTAGTCAAGGAAATTTCACTGCGTTCCAATTCTTTCTTCGAGGCTCAGGGGCAGTTACAGGATTTAAATGTGAAGATAGTGGAGGGATGTAGCCGGATACGGCAATTAAAGGAGACCATACGGCTCTTGGATGTCGATTTAGTGGACTCTGCCAGGGAAATTCAGGAGCAGAATGCGACCAGGAACAATTTATTGGCTCTTCAGCAGAAGTTGAAGCTTATTTTATATGTTAATCAGGCCATTTCAGCTCTTAAATTGGTATTTTAATCAGTCCATTTAATCATACGTATGGTACTATTTGCTTGCTATAATTTCTTTGCGTTTCTTGGATGAACCTTTAATTCCTAAGTTTTTACCCCGTCTACTTAGTTGTGGTCGATGCATTAAGTCATAGAAAATAGCAACTGTTGTCAGAGTCTGTGCTTTTTCATAACAATCCGACGGCTTCAAAGTTTATAATTGGATCTTTCAGAGCACAATTCTATGACCATGTTCAATCGTGAAGCGGGTACTGATTAAAAAAACAATGAAAATTTGCTAATACTTTTAGAATGATATGGAAAGGTAATGTATTCAGGACCTGTGCAGTGCTTTTCAGACTCTCAGTCATGAGACCACCAAGGTAATCTGGATGCGACGATATCCTAAGAAAATAAAGCTGGTGTGATTATTCTGTCAAGAGGTCTTAACATCCAAGATAAAATTCAAATGAGGAAGTCCTACTCTTGTCTTTCTCCAAGTTGGTGTATTGTGTTTAAAAGAAATTAGGAATTGACACATCACCTTTTCCTTTTGTGTCCTTTTAGCATGGGATTGTGGACAAGCCTGGTTGTACTGCTGTTAAGAAGGCTTGTACACGTGAGTCTGCTTTCTGTTGTTTTCATGACTTCTAAAAGAAAAGCCAAAATTTTATGGCTCAATGTCATTAATGCAATTTCTTGGGAGGTTTGGTTAGAAAAAAATAGGAGAGTATTTCCTAAAAAATAGGGGGATGGAGTCTTGATGAAAGTTAAGTTTTTGTTTCCTCCAATTGGAATGCTTTACACGGATACTTTTTTATAGTTATCATTTCTCTATGGCTTGTGCCAATTGGAATGCTTTACCTTTTGGTGGAAGATTAGTATTAATTTTTTTAGTTAAGGAACCTTCAACAAAACTTGTGATTGTGGAAGAGAGTCTATGTAATTCTCTTTACTCTTTATAAAGGTACAATATTGGCCATAGGACTCATATCTAGTAGTGATCGAAACTAGTATTAATGGATTTATACTTTTGTCGTACAGCTTGTAGCATCAGGAGATTGTGCTGGAGCCTTGGATGTCACTGATGATTTACTACATCTTCTGGTATTTTCTCATCCAGCGAAGTAGATGATTTTTATTTTTTAAATCTATATGTTTATCTGTTCTGTACGAGTTCTGTTCATATTCTAGGGTCGGAAAAAATAAATCTAAAGTGATGACAGTGTAATCTTGAATCTTTTGATAGGCTTTATACTGCCCCTATTAACGAGAACTAAGAAATAAATAAAATTTAATAAAAATGGAGAAACAAATTGAAAATCTAGGAGGTTGTCAGTCTCGCAAGAAAGCAGAAAAGAGATGACCCAGATGCCAAGATATGGATTCTAACTCTGCCTATCACTCAGAAGGGGGAAACTGAAAAAACAAACTTGGTTTACATTTCCATTTTGTACTCTTAATTTCACATTAGTAATGAAATTGTTTTTTCTGAAAAAAAGTTTGGTTTACATTTTTGTTGACAATTTCTTCCCGTTCCAAAAAGTTAGTATGTTGGCTTGAATCAAACAGAAGTTTCTCAGTTGATCTGAATGTAAATATTTATTAGTTCAATTTCTTATGTGCTTTAGGAGGGTGATGAACTTTCTGGTCTTCATTGCTTTCGCCATCTACGTGATCATGTAGCAGCATCAATTGAATCCATAACCAGGTAATATTTTAATCTTATTGTTGTACAAGTGGGAAAGAATCCGTCAACATGAGTATAGCTTAAAGGTAAATGACATCAACTCCTGTAGTTACATTTTGGGGAATCTCATCTATCATTACATTTTACAGCTTCCATTTCGAACCAAGCCAAGTTATACTGTAACCACCGACATATTAAACTTAGTGCTATGCCTGTTCCAAATAACCTTAATCATGAGGTTAATTGTTAAATTCAGTATGTTGGTAGTCATAATTGTCCATATCATTTCCTTTTTTATTGATTAAAAAAACTATACAATCATGTTAAGAGTCTGAAGTGTTTCACGTAATCCCGTTTGGTGCTTTTGTAATTTCATCCATCAATGAAATTGTTTCTTACTCAAAAAAAAAAAAAAAGGTTAAGAGTCTAGTTAGTACATCAATAGGTTGAAAAGTTTATTTGTGTGATTTTTATAAATGTGACAGGCAAGATATTGTTGCTTGTTTCTTATTTTGCATCTTATCGTTTTTCGTAACTTCGAAAATGAGTTGCTATCAATGTAATATACTGCTTTTATTATTCTATTTTTTTTAATTCCCTCTGGAGACTATTAATATATTAATTTTCTGGTGTAGCATTCTTTCAGCAGAATTTATGCGTGCCTCATTTCATGATGCTGGAGATGTAGATCTCGTTATTATATTTGAAACAAAAGCAAGGGCTTCAAATCTAATGAATGGAAAGGATGAAGTAAGTAAATATTAGTATAAAGTGCCTCTAAAACATTTTTTATTCTAGTTTACCGATGTGTTAACAGGGAAGCCTGAAGGCTCTTCCCGACCCTGTCAGGGGAGATGCCAAGTATCTCTTCAATGCAGTAGATTAATTAATGCAATGTATTATTACTTTGAAACGAATTTTTGTACATTAGCTAAATGACCTCAATTATAATTAAACAATTTTTATGTCATCTTCAAAAAAAGACATGTCTTGCTCTCTCCTTCCCCTCTCTTCCTCCCTCCCCACAACTCCACGCCGTGAGCACTAATCCACACCCTTGTAGCCATGGCCCAGCAGCTTTCAGTTCGCCCTGTTTTCATAGAACGAAAACGTTTCACGATAGATAGAGCCTTGTTCCTAACCATCCAGCGATCCTCCCTCACCTGGCTAAAGAACATCTTCTCAATCTCTTCTCAGCATTCCCCTGATGACCATACAATCTGGATAGAGAAAACTGTTAATAGGAAGGGACCCATTGTTGAAATCGCGAAACTAGACAACAATGGTGGTTTCAACAAGCTATTGGTTCCATAGGTGGGGAAAGAAAAGGATGGACGACTTTTTATAACCTCTTGAATGCATCCTCCTTTAATACTGCACAGCCAAAACTACCAATATTGCTACCTTTTTTGAAGTCGTTAAGAAAGGAAAAGTAGTTGTGAGAGAAAAAATCCCTCCCTGCATCACACCATCCTCCCATCAACAGCATTTAAAGACTCACGGCCCAATAGACCTCTATCCAGAATCAGACCCCCATGGCTCGAATCCTCCATCATTGTCATAAGGAAACACTTCCATGACAATTGGTTCTGTATATCTCGTGCTCTGCAACAACATATCAGTCACTTCTGTACTGTCAACCCCTTCCCTGTGGACAGGGCCCTCCTTCGATGTGAAAGCGAGGACCAAAGGCTTACTCTGTGTGGATTAAAGGACTGGTACAAAGTCGGTTGATTTCAGCTAAAGTTTGCTCCATGGAGCCCAGAGGCGGCAGCACTTTACCCAAAAGTTCCCTCATACGGGGGATGGATAAAGATTAGAAACCTCCCATTAGATAAATGGGATAAAGAAACCTTTAGGTTTATAGGAGATTCATGCGGTGGATACGTTGAAACGGCCAAAAAGACCCTGTCCCGGCTTGATATTTTTGAAGCCTCCATCAAAATCAAGAATAACCCATACTCTTCTCCTCCTCTGATGATCTTTCCCTTACCAACTTGTTCAGCATTGTCCGACTTTTTGAGGAAGCATCGGGGCTGAAAATCAACCATAAGAAATCAGAAATTATGGGTGTAAACCTTGAGGATCAAGAGCTTGACTACATAGCAGAGAAGTTTGCCTGTAAAAAGGGATTTTGGCTCAATACTTATCTTGGCCTTCCCCTGAACAGTAAGTACAAATCACTCTCATTCTGGGATCCCTTGCTTGAAGAAGTGCAGAAAAGACTCCTATCATGGAAAAACTCCTATATATCCAAAGGAGGTAGATACACCCTCATTCAAGCCACCTAAACAACCCCTCCCCACATACTTTTTTATCCCTTTTACCCTTGCCTTCCAAAGTTGCAGCTTCCCTAGACGGCATCTTCAGAAAATTTTTGTGGAAAGGAAGTAAAGCAGACAAAGGTATAAACCTAGTTGAATGGGAGACCATCACTTGGCCTATAGAGGATGGGGTCTCGGCCTATATGGTATGAAGCAAAAAAACAGAGTGCTACTAGCGAAATGAATTTGGAGATATTTAACAAAGCCGAATGCCCTCTGGAGTAAGCTGATTGACGCCAAATATGGGTATAACACAAGGAACCACAAATCGGGTACTTCATCTCTAGCCACTGCCAAAGGACCATGGAAAGCCATCATAAAACAACAGCATCCAATTTTCGAGCATTTAACTTATTCTATTGGAAATGGACAAATCCTTTCCTTTTGGCATAATCCTTAGACAAACGGCGGACCATTGTCTCGTCAGTTCCCCCTCCTGTTCAATCTGACTAGGGTTAAAGAGATGACAGTGAAGGATGCGTGGAATGAGGAAAATAGAAGCTGAAATCTGAAGCTGTAAAGGAACTTGAAGGAAGAAGAAATTGAGGAATGGGCACTCCTATCCCTCCACCTATCAAGCATCAATCTCGCTGCTAGGAGAGAGTCATTCCTTTGGAACCCTGAACCAGGAGGCAAATTCTCGACAAAATCTCTCATGAAAGATCTTCTCAAACAGCCTAATGATTCCTCTCCCCTGCTGTTCGAATCAATTTGGCAGGACCCCTATCCCAAAAAAGTGAAGTTCTTTATTTGGGAGCTCAGTCACAGGGCCAGCAACACAAACGATAAGTTGTAGCAAAGGTTCCGCTCCTTGGCTATCTCCCCACAGTGGTGTCCGATCTGTAAGTACAACTTGGAATCACAGCAACATCTCTTTTGTCACTTGTGTCTTTGCCACACGATTTTTTGGTCAATGATTCTCACAACTTTTGGCTGGTCAATTGCTCTTCCAAATGATCCTCCCCTCCTATTGGAAACGACTCTCCTGGGACATCCCTGCAACAACTAAAAGAAGCTTTTATGGTTGAACATTAACAGGACCTTCTTGTGGATCTCATGGAGGGAGCAAAATCAGCATATCTTTAATGGAAAAGCTCACTTCTGATAGACTTTTTGAAGATGTAGTTTTCTTGGCTATATCTTGGTGTACATGCTCACCCCTCTTTCACAACTATAGCTTCTCCTCTCTATTGTTCAGCTGGAATAGCTTAATGTAATCCCCTATCATGGGCCCACTTTTGTAATTTCTATCATCAGTGAAATTGTTTCTTCATCCAATTTTTTTTTTATGTCATCTTCTACAAAATAAGTTAATTAAGCAGTAAATTTGATTCTGCTAACAACTACATATGATTCTGTGTATCTGAAACTTCTATTCACTGTTTGTAACTGCTTTTTGTCTGCATCTTATTTCTGTTATTGTTACTAATCGAGGACTAGTTTTATAATTTTATTGCATGTGACACGTGGATAAAACTGTGGCAGGTAAAGTTTGATGAAGAAGAAGCCTCCAACTTCCGTGATCGCCTTCTTCCTATCATCATTGGATTGCTTAGGACTGTGAGTTCTCATTATCATGCTACATTGTTTTGGTTGGAGAATAATTTCTTGATCTTTCCTCTCTGATTGAACGTTGAACATCCCCTTCATTTGTACATGAAGATCCATAACTATCAACTACCTCGTCATTTTATCTTGCTAAGTGTAATAATCCCGATGGGAACTGATCGCCCACAATTTTGTAGCAACAATTCTTTCTCCCCCCCCAGGTTTATGCAATACTGTTATGTGGTTAATCTGTCTTATGGTCAGTTTAAAGATGTTGATATGTACTCTAGATAGGGTTCTTGATATGGCTTGATTACGAGCATTAAAGTTATTTAACTGTTGGTTTGGAAGCCTGTACATGGAGTTATATGATCTTCAAATATTGATTTTCAATTCTGAACCAAATTAATTACATATATATGGTTGTTTCTACAGGCCAAGCTTCCCTCTGTGTTGAGGTTATATCGTGATGCAGTTACAGCTGATATGAAAACTGCTATTAAGAATGCAGTGGCAGAACTACTTCCTGTTCTTCTAATGAGACCTCTAGACTCAGATTTTGCACCTGGAGAGAGAACGGTGGATGCAGATGGTGAGGAGTAGCTGAATCTTGATATCCATCTGTTTGTACAATATTACTGTTTGATTATTCTTTTCTTTTATGAAACAAAATAGTTTCATCCATATTTTTGTGCATGATACGAAGTAATTTTGCTAAAGAGGATGAATTGCACAAAAGATTAAATGAAGAAAGTACAACTGATCAACAGCGTTGTGAAATTATATTACCAATAAGGTGATAGGCTATAGTTGCGTTTTGAATTGTCATGACAGACGTAGTGTCCTTCAGCATTTGTAGGATTTATCCTATTTCATTACAAAATATTCTCTCAGATCTAAATCTACATTTCTTATCAAGATACATTCTTTCGTGATGAAATTGTGTGTCATCCAGTCTGCAATTTTTAAGTTTCTTTAGTGCTTCTTTATTTCCCTGTTGTTCTCTTTTATGTGAATGATATTACAATAAATTGATGACTCAGGTGGAGGTGCATCACTCGCAAGCAAGCTGAGGGGTTTGTCATCTGAAGGCTTTGTTCAACTTTTAAGTGCCATTTTCAAGATTGTACAGGTAATTTTACTTGCTTAAATGTACTCTCTCCATAATGCACTCAATCTGTCCTACACTGCTTCTAATTATTAAAACATTAGATTTATTATATCAAATGTACGGGCTTGTTACATTAAAATAAAGTTACAGTTTATGATTGATGTGGTTTTGTAATTTGTTGGGAGTTGGGCTGCGGATAGAAATGTTAATATAAGAAAGTAAAAGAAGCAAAGGGTTGAAGTTCCAGAGTTTTGGATGTTGAAATCAATCGAAGCTTGAGACAGATCGTGAAACTGGCAAGCTTGTTAAATCGGAGATTCCTTTGGCCTTCCCTCAACTATATTTTTGGTTACTTAGTTTGGTGTAAGGTTCTTACAAAAAACTGGTTTGGTGCAATGCTAGAGTGGATATCCCATCTTCATCTCTTTTGTTTTCTTTCATTAATGAAAATACTGTCCTACAAAAAAAAGGTATGGTGTAGGGTTGCTGGTTCAATTAGCTTTCCTGATTCTAGTAGGGGTTTGGATTCTCTATCTCAATAAGTCCTTCTGATTCTAGATGGACATTTAAGGTTCAGTAATTGCTGTTTGTTGTTTTGGGTTTTATCAGATTAAGTAGTGGTTTCAGGTTTAGGAGTGGCTGTCTTTGGCCCTAAGATTGTCATTTGAGGTCTAAAGGCATGGGTCTCCGCCTCTCTTGAAAGAACTGGATGAAACGAATTAAGGGAGAGCAAATTCCAATCTTCTTCCCCAGTTTCTTCTTGAAGACAGATTGGAAAGCAACACTTTTATTAACAAAAAAGGGAGCATAAAACAAGGTGGATGAAGCATTCTCTTGAAGGGCAAAATTAGTTATTAAATTGCTCTTCAATTGGCTTTGAATCACAAAAATACTAGATCATACTATTTTCTTCTTCAATATAAAGATTGGAGGTAAAAATCTTCTTAATCTAGTTAAGGCATTTGAGCATACATCTGGTTTGAGAATTACTAGCCTACCCAAATAGGTAGTAGTAGGCATTATTTCTTTTCTTTTTTTTAAAAAAAAAGTTTTGATAACGCAGATGTTTTGTAATTACCAGTTAGGTGTTATTCTTTTGGATCGGGTCCCTTGTTGTAGGCGGATTTCCTTTTGTTGGTTGTTATTTTTTTTAATTGTTATTTTTTTTATGCCTTTGTATGTTCTTTCACGTTTCTCAATGAAAGCTCAGTTTCTTATAAGAAAAGAAACAAAAATTCGATAAGGTTGTAGTAGGCATTATGATATGTGAACTTAACACCTCAATATAGGACCTAAATCAGGTTTGTCATTTGGTAGTGCAACAAAGCAGATTGATTGGTCCATTACTGTTTTAGCATCAATTGGAAAAGTTTCTTGAACAGATTAAATGAAATTAATCATATTTCTAGAGGTCTTCTTACCCTAAGTTTTGCACCATGCTGGAAATTCATCGGATGCATTATGATCTCTTTTCATTTAGTGAATGCAGGTTCATTTAGTGCGTGCTGCAGAAGTGAAAAAGTCTATTGAATGGATTATGTGCAACCTTGATGGGCATTATGCTGCAGATTCAGTTGCTGCTGCAATTGCTATTGGTGCTGCAGCTGCTGGTACAGCTCAAGATAGTGATAATCAAGTTGGTTTGCTTCTTCCACATTTACCTCAGCGAGTAGCTGCCAAGGTTACTTCCTTACCGGGAAAAGCAAATGATGCAGCAAATCCTTCAAATATGTCTAGAAATTTCCGGTAAAATATTGTTTCGTTGTTTTGATATATTTTACTTTTCCTTTTTCTTTTGGGGGACGGGGGGAGCTATGTGCCAATTTAGTCCGAGTAAAACTAAGTAGTTAAAATTTTGTACCACTCCGTACATATCATACTTGATGTACAGAAACTTGCATCTACTCTCGCTCTCTCTTTTCCGCTCAGTGCTATAATTTTGGTTGAAAAAGAAGAAATGTTTGAACGGTGGAAAAAGTGCTCTCAGTTTAATTTACCAATAGAGGTGTTGATCTAGGACACTGGCCTTCCTCTAAGTAGTCATAATTCTGGAAGATTGGATATCAGAAATATGACAATAGAGTAGTTTGATTCTTTCATTGTTCATCTTTTAGTCAGAAAGCTTGAGAAAGAATAAAATTAAAAATTAGGACACTCTTACTGTTACTGCATACTGCCTACCCGCTGCGCCACCAATAGTTGTTGTGTTCTAGGATAGCTAAGAGTTGCCTGTGTTAGCAATGATCTTAGCTTGAATTAAATGCTTTTGAACTCGCCTTCTTTCTGAAAAAAACAATAATAATCCAATCTATCTGGGTCTGGTTCTATGAAGCACCATTGAGAATCGTATTCTTCATTGTTTTTCTTTCTTCTCTGTTTTAAAAATTGCTGCTCACTTGCTGAATATTTAATTTTGATACTTCCCATCTTTGCTTTTATAACTTTATTATTATTCTCGACTGTTGAAGCAATTTGATTGTAAACAGAGCTGATGTACTGCGAGAAAATACAGAAGCTGTTTTTGCAGCTTGTGATGCTGCTCATGGAAGATGGGCTAAACTCCTTGGTGTTCGCATTCTAGTTCATCCAAAGTTGAGACTGCAGGAGTTTTTAAGCATATACAACATTACACAAGATTTTATAACTGCCACTGAAAAGGTACCAAAAGTATATCTGGAATATGAACTTTTAAGGATATAAATCCATAGCTAATCAGAATATTCATGATTCCATCATTCTTCTAACTTAAACAGATTGGTGGAAGGTTGGGATATAGCATTCGTGGAACTTTGCAGTCACAGGCCAAAGCTTTTGTTGATTTTCAACACGAATCTCGTGTAAGTTTCTGGTTTTCTTTCTTTCTTTATTCTCTTGTTTTTAGAGTTATTATTGTACCTAACTCTCTTTTGTTGAGTTATTATTCATGGTTCTGTAACTTATGGCATTGTAGATGACAAAAATAAAAGCAGTGCTTGATCAGGAAACATGGGTTGAAGTGGATGTTCCTGACGAGTTTCAAGCTATAGCTGAATCACTTTATTCTCAAGAACTGCTTTCTGCAAAAATTGATGATGCTCAGGGTAATATGGATCGAAGCTACACTGATGTGACTACAAATAATGATGATTCAAGCATTGTAGGGGGTGGATCACTAAATGCTCAACAGCACTCTGAGCTGACTGATTCAAGTGACATGACGGGGGGGAATACTGAACATGCAAAGCCTACTCCTGCGGACAAAATTGAAAAAAGTAAAGCTGATGTGTTAATTCCTACAACACAAATTAACAATACTAATGTGAAGGAACGTGGAAAATCAAGCTCTCAGACTTTGCAATACAAAGGCATTGGTTATCACATGGTAAACTGGTTAGTTTCTTCTTTCTTTTATAATTTTTTCTCGATTATATATCTATTACTTGCTCCCTCCTCCACAAACATCCTTAAATAAATTGGTTGGTGCCTAGTTGTTGGCCATGACTTAACCTATTTGGTCCTTTGTTGTTATTCTGTCTTCATTTGGAGCTTCTTTATTCCTTGTGATATCACATTGTCACATTGAATTATATATTCTATTTTTTCTCCATAGTGCAACGGTACTGATGTACACCATCGTTTATTAATTTTAATTAGCTTGATGAACTTTATTTTGTTTGCTGCTGGTGTCTCAAATAGTATGTTTCTTCTTTTATGGTTCTTTCCCAGTGGGTTGATCTTGCTCAAGATGTTGTCGGAGTACATTGACATGAATGATTCTCTTCCAGCACTATCTTCTGAGGTCGTTCATCGTGTTGTGGAAATTCTCAAGTTTTTCAATACAAGGACTTGTCAACTTGTTCTTGGAGCTGGTGCTATGCAGGTACTTCAGTTAGACTCAATGTATGTAGTCAATGTAATGCTTCTATATTTGCTTCTTTAGTTTTTAATTTACTTGTAAATATGAATCCTTTTAGACTGTTAAATCATTGAAAATAATGTGGATTTTTCTTTTTTCTTTTTTTTCCCTTAATGTGTAGTTGGTTGAATATTATAAATCATTCTTGAATAGCGCTTTCTTGTTCTTGCATAGCGCTTTCTCTATCTTGCTTACCATGGAAGCTATGACTTTCAAATGCTAGGTTGGTTACTCTCCCTCCTAAAAACTGAATTTATGTGGTGTTCCAGGTGTCTGGTTTGAAGTCCATCACATCCAAGCACTTGGCACTGGCCAGTCAAGTTATCAGTTTTACTTGCGCGATTATTCCTGGTACGTTGCAACAAAATCTTTAAGGCTTCAACTTTAATTCCCTATTGTTGCTTATGTCGCAGCCTATATTTCATAGGCAAGGCCAATCAATTTTATGTCTAGCAAACCACATTCTGCCCCACGCTCTTTGTCTTCAGCACCTACTTCAATTAAAAAGAAATCTTCTCATATAATTATTCTATTTTTTCATCATTGTTTTTTAATTTAAAAGAAACCGAGCTTGTATTGGAAGAAATATATGCAGATAAAGGAGGACATAAATTCTCTCAAGAAGAACTAACAGGATCTGATCTAATTGGTTTCTTAATATTTAATAAAATGTTTATGTTTTTTATGAAACATTATTTTTCATTCCATTTTATTGACGATTCCAAGGACATTTTTAAGTTTTGAAAGTATGATGAATGAAAATGTTGACACTCAATCGTGCATGCTTGATACTTGTTGGGAACTTTCAGAAGCATGTTGGATGTAGCCGCAGCCTATACCTCCTGTCGTGCTTTGATTGAATTTAATAAAAAAGAGAGAATTTAAATATTAAACAAAAGAGAGGATTTAAATATTCCCAAGGGATATAGCCATTACTTCCTTCTTGGTAATATTCCTTCTTCCAATATGAAAGTCATGTCAGGCTTATCTAAGATTGACTAAATATTACAGAGAAGCAGGAAAATGCTTTGGGAGACATTGTGTTTCAGAATATAATCTTTTTCCCTTTTATGTGATGTATCTGACACAGGATTCCATGTTCTTTTGCGGTGATTTGGTGAATATGGGATTGGGAATGATATTGTATTGCTCAAGATGTTTTGAAGTAGTTTGACGTTTTTTTTTTCTTTTTGTGTCTTGACCAAGAAATTAGGAGAATACTTTTTCTCAAGGTACCTGAGGCGCGAAAGACACTATTGATCTCAGAGATTGATCGAGTGGCTCAAGTATTTATTTTTTCATCATTTCATTCCTTCTGTTATATATCAGTTATGCTAATTGTATATGTTGCTTGTCCCTGGGGCGGAAAAGGGTTCAAATAGTTATACTTGTAGTGTTGTGCCATGTGTGAGCGTGTGTTTTTTAAAGTGAGAATTACATTGAAATCCAATAGTCCTGGCACTTGAAGCACAAGTTTTGAGAATAACCACTACATAACCCAATGAACCAAACCTAAAAATTGCACATGCAATTCACATTTAACTTTGATTGAGCAGTAAATGACTAAAGAGAGCAAACTCTCATCAAATATGCAAGTATTTCAATCTTTTTGGGTGTATGGTGAGACATAGAAGAGTCTGAGAAAAGTTAAAGGAAAAGAATATACATTTATTGGCCATCCTGGATGGTTGGTTATTTTCAAAGATGAATCTACTTTAATTTTATTATTATTATTTTTTTACAGAACTTTAACTTTAAATTGTTGGTGTTAGAGTTGCATGTATATATAGAGTTTGACTTTTTTCTGGAATTGTTTCTAGATTCTTGTCCAAGTCCTTGTATTATTACTCTTGAATAGCTTTCTCTTATAAGATTTTCTGTTATGAAGAATTGCTTTGTTACGTACTTGCACCATTTATTCAATTATTTAATAATTTAGGATTACAAAGTTCACCGAGATGAAATTCATACTAAGCTGGTCCAGATAATGAGGGAGAGGTTATTGGTTCATCTTCGTGGCCTACCTCAAATTGTTGAAAGTTGGAATAGACTCCAGGATTCTGACCCTCAGCCCAGCCAGTTTGCTCGATCCCTTACCAAGGTAGCTACTTGAATTTATCAATCAAACCAATGCATAGAAGTTTGATAAGAGCCTTTTCCTCTAGTTACTTTAGATATTTGTCCTGGGTATCATGTAAAATTAAGGGATTAGTTCTTTATTTTCAATATCAAAGTATTTTCTTCCGCTTTGCATGCTTGAACACATTGCATGTACGAATGGTTATGACAAATGAAATCCAACATGGTCGGTGCATAATAGCACAAGTCATCTCACAAGTTTGATTAGAATGATCTTTGCATTTAATTGCACTTTTTGCCTTGTTTGTCAACATGTTTCTGATTGACTTGGGTTGAAATCACTGATATCCAGGAAGTTGGGTACCTTCAGCGTGTCCTATCTCGAACCTTACATGAGGCTGATGTTCAGGCAATATTTAAGTAAGTCATACTATGATGCATTTGCTAAATTTTATTCCAGACATTTCTCCTCTTATTATGTTGAAAATTGTAGGTGTTTCATTTGTTTTTACAGATTAAACATTTCATAATGAATATATTTTTCATGCATGGAGAACTAATAAAAATTTTTCTAGAGTTTCTCTGTGAAGGTGATTACTACCAAAGATACATATAATGGTTCCTTTTAGTAGATGAGTTCTGAAGCAGATCAATATTGAGATACATATGTTGGTTCCTTTAGTAGATATGTTAAGCAACCTCTGATCTTATAATAGGGTTGTTCCATTTTAAAAAAAAAAATGGATTGCTGCTAAACATCTGTAGATCAAGCATGTTTTCCACATTCTACTACTTGGAAGTTAATAATTTTCTTTGGTGCTACCTTCAGGCAAGTGGTCAAAATCTTCCATTTACAAATTTCTGAAGCATTTTCACGGTTAGACATAAGTACCCCTCAAGCAAAGGATAGGTAATGCCCCTTTGTCCTTTGGAATCAAGGATATTATCACCGAACCATTATTACAATATGCTCCTTGTTCTGACACCCTTTTTCTAATTGGATACCAGGCTGCTTCGGGATGTTAAGCACATTCTTGGCTGCATAAGATCTTTGCCTTGTGATGATTTGAGTAAACCAGACACCCCAAACTGGGGGCAACTTGATGAATTCTTGGATCAAAGATTCGGATCTGAAGCTGGATAATGATGTGAACGTGGAATGGCTATTGTTTTCCTTTTTGATGAAGGGTGTCATATTTGAGGAGTAAATATCAGGATATCAGAGTCAATTTTCGACCTATGTCATCTCCCTTGTACAACTAAAATATCAAGGTATGATGTAAACAAATCCTTATCCTATAGAATTTTGATGGATTGTTGGCAGGTAAAAGTTTAATCACCTTCGAGATCAAATCAAAGTTATCATACCCATAAGATTTATGAGCACTTGACAGTTTTTCGTTTCTAAAACCTCTAGCGGTAGTGTAAGAAAAACTGGTACTTTCATATTTGCACTATTTGTGAGCACTTCTTTAGTCTTCATTTCTCGTTTTAATTTGAGGAACTGCATTGGCCTCCAAAACTTCATTTATATGTGGCTCTGCGCTGAATCTTTCATGATTGATACTACATAGCAAGTTTTATGTTTCTACTACTAAATTTTGGCTGCATAAATTTCTGTTGTAATGTCTATTAGATTTCTATACTTGCGTAGTGAACCTTACCTTTTTGTTACTTCGTATACATTGGCCACTTTGTACTCTCAACTCCAGATCAGGACTACTTGTATCAAATGAGATTGTAACACTTGATTCGCTGGTACTTTGACTTGATTTTATTAGCAATATCATCCGAATGATTTCTGTTGTGTACTAACCAACGGGAAACTTCCAGGTTTTCGAATGCGATGGACAGGTATAAAGTAAAGTATCGGATTCAGATATTTCAACGAAGTTTATCCCCCTTGCCCTTTTTGCTGAAACGCATAACATATTTGTTTGTATTGGCTAGGCCAACGCTTTTTGCTGCCCTAATTGTAGTTATGATGTCTAGAGAAAGAACAGATCAATTGGTTTACTTGAGAAGCTTTAACCAGAATAGATTTTAGTTCCTTTTTCTTTTTTCCTCCCTCCAATTTCAATTGGGTTTTCCGTACAGGTATGCATGTTTGTACTTTGTAGAACAGAATTTATCTTTATTGCTATAAATCAATTATTTTTTTTTCAGTTTCAACTAGCACATCTACTTCTGTTGCGTAGAAGATAATTTATACTTATTT

mRNA sequence

CGTTACAATTATATTATAATATTACAAGGTAATGTGTAATTGCGTAATGAAGGCCAAGGCCAAGGGTGTGGAATCGGCAGGTGGAGGTGGGCAGTGATGTGGTATAGGAATGGATTCCATTTCATGAGCCCAGAAATTTTGAACGAGATCTACCACCGGAGTTGCATTGGAAGGAGTTCGTTTTTCTTTCCTTCATCCAGCGCCAACTTCCTTCTCTTTTCGTGGCTTTTCTTCGTCGTCTGTTGAGATCTGGTCTGAACCACTTCATCTCGATCTCTATCGCTTTGGAATTCGACTACTGAACGTGATATTCTGTCTGGAACCCGCCATTCATTCATATGATTCTCAGATTTTGAGCATGGAGTCCCAGCCTTCCCAATCGGGAAGGTCCCCTACTGAATACTCCACCCTTCTCAGTAGAGAAACGAGTCTTCGTCGAACTACTTCCTCTAAAACCAACTCCGATGCCAGCAGCCAGAGCTTGTCTTCAATTCTCAACAACCCGCACGCTGGCAAATCCGATGCCTCTTGGGCTGCCTGGTGGTCGTCTTCGTCCACCGTCAGCCCGCCTGAGTTCTTGCCCCTCTCCTCCACCATAGCCTCCTCTGAAGTTACTCGATTCGATTTCAACAACTACATCGCCTTGATCTCCGATTCTTATCATCGATTCGAGGACATACGGAACCATTCCACCAAAGAGAGCGTCGGCTTGGATAGCATTGGGGGCCAGGGGGAAGCGCTTGTGGCATGTTTGAGAGAGGTTCCCGCGCTTTACTTCAAGGAAGATTTCGCATTGGAGGACGGGGCGACATTCCGTGCAGCCTGTCCCTTCTTGAATGTAACGCAGAATTTGGTGCTCCAAGAGAAGCTTTCACATTATTTAGATGTCGTGGAGTTGCATTTAGTCAAGGAAATTTCACTGCGTTCCAATTCTTTCTTCGAGGCTCAGGGGCAGTTACAGGATTTAAATGTGAAGATAGTGGAGGGATGTAGCCGGATACGGCAATTAAAGGAGACCATACGGCTCTTGGATGTCGATTTAGTGGACTCTGCCAGGGAAATTCAGGAGCAGAATGCGACCAGGAACAATTTATTGGCTCTTCAGCAGAAGTTGAAGCTTATTTTATATGTTAATCAGGCCATTTCAGCTCTTAAATTGCTTGTAGCATCAGGAGATTGTGCTGGAGCCTTGGATGTCACTGATGATTTACTACATCTTCTGGAGGGTGATGAACTTTCTGGTCTTCATTGCTTTCGCCATCTACGTGATCATGTAGCAGCATCAATTGAATCCATAACCAGCATTCTTTCAGCAGAATTTATGCGTGCCTCATTTCATGATGCTGGAGATGTAGATCTCGTTATTATATTTGAAACAAAAGCAAGGGCTTCAAATCTAATGAATGGAAAGGATGAAGTAAAGTTTGATGAAGAAGAAGCCTCCAACTTCCGTGATCGCCTTCTTCCTATCATCATTGGATTGCTTAGGACTGCCAAGCTTCCCTCTGTGTTGAGGTTATATCGTGATGCAGTTACAGCTGATATGAAAACTGCTATTAAGAATGCAGTGGCAGAACTACTTCCTGTTCTTCTAATGAGACCTCTAGACTCAGATTTTGCACCTGGAGAGAGAACGGTGGATGCAGATGGTGGAGGTGCATCACTCGCAAGCAAGCTGAGGGGTTTGTCATCTGAAGGCTTTGTTCAACTTTTAAGTGCCATTTTCAAGATTGTACAGGTTCATTTAGTGCGTGCTGCAGAAGTGAAAAAGTCTATTGAATGGATTATGTGCAACCTTGATGGGCATTATGCTGCAGATTCAGTTGCTGCTGCAATTGCTATTGGTGCTGCAGCTGCTGGTACAGCTCAAGATAGTGATAATCAAGTTGGTTTGCTTCTTCCACATTTACCTCAGCGAGTAGCTGCCAAGGTTACTTCCTTACCGGGAAAAGCAAATGATGCAGCAAATCCTTCAAATATGTCTAGAAATTTCCGGTATTGTTTCGTTGTTTTGATATATTTTACTTTTCCTTTTTCTTTTGGGGGACGGGGGGAGCTATGTGCCAATTTAGTCCGAGTAAAACTAAGTAGTAAAATTTTGTACCACTCCGTACATATCATACTTGATGTACAGAAACTTGCATCTACTCTCGCTCTCTCTTTTCCGCTCAGTGCTATAATTTTGGTTGAAAAAGAAGAAATGTTTGAACGGTGGAAAAAAGCTGATGTACTGCGAGAAAATACAGAAGCTGTTTTTGCAGCTTGTGATGCTGCTCATGGAAGATGGGCTAAACTCCTTGGTGTTCGCATTCTAGTTCATCCAAAGTTGAGACTGCAGGAGTTTTTAAGCATATACAACATTACACAAGATTTTATAACTGCCACTGAAAAGATTGGTGGAAGGTTGGGATATAGCATTCGTGGAACTTTGCAGTCACAGGCCAAAGCTTTTGTTGATTTTCAACACGAATCTCGTATGACAAAAATAAAAGCAGTGCTTGATCAGGAAACATGGGTTGAAGTGGATGTTCCTGACGAGTTTCAAGCTATAGCTGAATCACTTTATTCTCAAGAACTGCTTTCTGCAAAAATTGATGATGCTCAGGGTAATATGGATCGAAGCTACACTGATGTGACTACAAATAATGATGATTCAAGCATTGTAGGGGGTGGATCACTAAATGCTCAACAGCACTCTGAGCTGACTGATTCAAGTGACATGACGGGGGGGAATACTGAACATGCAAAGCCTACTCCTGCGGACAAAATTGAAAAAAGTAAAGCTGATGTGTTAATTCCTACAACACAAATTAACAATACTAATGTGAAGGAACGTGGAAAATCAAGCTCTCAGACTTTGCAATACAAAGGCATTGGTTATCACATGATGTTGTCGGAGTACATTGACATGAATGATTCTCTTCCAGCACTATCTTCTGAGGTCGTTCATCGTGTTGTGGAAATTCTCAAGTTTTTCAATACAAGGACTTGTCAACTTGTTCTTGGAGCTGGTGCTATGCAGGTGTCTGGTTTGAAGTCCATCACATCCAAGCACTTGGCACTGGCCAGTCAAGTTATCAGTTTTACTTGCGCGATTATTCCTGAAATTAGGAGAATACTTTTTCTCAAGGTACCTGAGGCGCGAAAGACACTATTGATCTCAGAGATTGATCGAGTGGCTCAAGATTACAAAGTTCACCGAGATGAAATTCATACTAAGCTGGTCCAGATAATGAGGGAGAGGTTATTGGTTCATCTTCGTGGCCTACCTCAAATTGTTGAAAGTTGGAATAGACTCCAGGATTCTGACCCTCAGCCCAGCCAGTTTGCTCGATCCCTTACCAAGGAAGTTGGGTACCTTCAGCGTGTCCTATCTCGAACCTTACATGAGGCTGATGTTCAGGCAATATTTAAGCAAGTGGTCAAAATCTTCCATTTACAAATTTCTGAAGCATTTTCACGGTTAGACATAAGTACCCCTCAAGCAAAGGATAGGCTGCTTCGGGATGTTAAGCACATTCTTGGCTGCATAAGATCTTTGCCTTGTGATGATTTGAGTAAACCAGACACCCCAAACTGGGGGCAACTTGATGAATTCTTGGATCAAAGATTCGGATCTGAAGCTGGATAATGATGTGAACGTGGAATGGCTATTGTTTTCCTTTTTGATGAAGGGTGTCATATTTGAGGAGTAAATATCAGGATATCAGAGTCAATTTTCGACCTATGTCATCTCCCTTGTACAACTAAAATATCAAGGTTTTCGAATGCGATGGACAGGTATAAAGTAAAGTATCGGATTCAGATATTTCAACGAAGTTTATCCCCCTTGCCCTTTTTGCTGAAACGCATAACATATTTGTTTGTATTGGCTAGGCCAACGCTTTTTGCTGCCCTAATTGTAGTTATGATGTCTAGAGAAAGAACAGATCAATTGGTTTACTTGAGAAGCTTTAACCAGAATAGATTTTAGTTCCTTTTTCTTTTTTCCTCCCTCCAATTTCAATTGGGTTTTCCGTACAGGTATGCATGTTTGTACTTTGTAGAACAGAATTTATCTTTATTGCTATAAATCAATTATTTTTTTTTCAGTTTCAACTAGCACATCTACTTCTGTTGCGTAGAAGATAATTTATACTTATTT

Coding sequence (CDS)

ATGGAGTCCCAGCCTTCCCAATCGGGAAGGTCCCCTACTGAATACTCCACCCTTCTCAGTAGAGAAACGAGTCTTCGTCGAACTACTTCCTCTAAAACCAACTCCGATGCCAGCAGCCAGAGCTTGTCTTCAATTCTCAACAACCCGCACGCTGGCAAATCCGATGCCTCTTGGGCTGCCTGGTGGTCGTCTTCGTCCACCGTCAGCCCGCCTGAGTTCTTGCCCCTCTCCTCCACCATAGCCTCCTCTGAAGTTACTCGATTCGATTTCAACAACTACATCGCCTTGATCTCCGATTCTTATCATCGATTCGAGGACATACGGAACCATTCCACCAAAGAGAGCGTCGGCTTGGATAGCATTGGGGGCCAGGGGGAAGCGCTTGTGGCATGTTTGAGAGAGGTTCCCGCGCTTTACTTCAAGGAAGATTTCGCATTGGAGGACGGGGCGACATTCCGTGCAGCCTGTCCCTTCTTGAATGTAACGCAGAATTTGGTGCTCCAAGAGAAGCTTTCACATTATTTAGATGTCGTGGAGTTGCATTTAGTCAAGGAAATTTCACTGCGTTCCAATTCTTTCTTCGAGGCTCAGGGGCAGTTACAGGATTTAAATGTGAAGATAGTGGAGGGATGTAGCCGGATACGGCAATTAAAGGAGACCATACGGCTCTTGGATGTCGATTTAGTGGACTCTGCCAGGGAAATTCAGGAGCAGAATGCGACCAGGAACAATTTATTGGCTCTTCAGCAGAAGTTGAAGCTTATTTTATATGTTAATCAGGCCATTTCAGCTCTTAAATTGCTTGTAGCATCAGGAGATTGTGCTGGAGCCTTGGATGTCACTGATGATTTACTACATCTTCTGGAGGGTGATGAACTTTCTGGTCTTCATTGCTTTCGCCATCTACGTGATCATGTAGCAGCATCAATTGAATCCATAACCAGCATTCTTTCAGCAGAATTTATGCGTGCCTCATTTCATGATGCTGGAGATGTAGATCTCGTTATTATATTTGAAACAAAAGCAAGGGCTTCAAATCTAATGAATGGAAAGGATGAAGTAAAGTTTGATGAAGAAGAAGCCTCCAACTTCCGTGATCGCCTTCTTCCTATCATCATTGGATTGCTTAGGACTGCCAAGCTTCCCTCTGTGTTGAGGTTATATCGTGATGCAGTTACAGCTGATATGAAAACTGCTATTAAGAATGCAGTGGCAGAACTACTTCCTGTTCTTCTAATGAGACCTCTAGACTCAGATTTTGCACCTGGAGAGAGAACGGTGGATGCAGATGGTGGAGGTGCATCACTCGCAAGCAAGCTGAGGGGTTTGTCATCTGAAGGCTTTGTTCAACTTTTAAGTGCCATTTTCAAGATTGTACAGGTTCATTTAGTGCGTGCTGCAGAAGTGAAAAAGTCTATTGAATGGATTATGTGCAACCTTGATGGGCATTATGCTGCAGATTCAGTTGCTGCTGCAATTGCTATTGGTGCTGCAGCTGCTGGTACAGCTCAAGATAGTGATAATCAAGTTGGTTTGCTTCTTCCACATTTACCTCAGCGAGTAGCTGCCAAGGTTACTTCCTTACCGGGAAAAGCAAATGATGCAGCAAATCCTTCAAATATGTCTAGAAATTTCCGGTATTGTTTCGTTGTTTTGATATATTTTACTTTTCCTTTTTCTTTTGGGGGACGGGGGGAGCTATGTGCCAATTTAGTCCGAGTAAAACTAAGTAGTAAAATTTTGTACCACTCCGTACATATCATACTTGATGTACAGAAACTTGCATCTACTCTCGCTCTCTCTTTTCCGCTCAGTGCTATAATTTTGGTTGAAAAAGAAGAAATGTTTGAACGGTGGAAAAAAGCTGATGTACTGCGAGAAAATACAGAAGCTGTTTTTGCAGCTTGTGATGCTGCTCATGGAAGATGGGCTAAACTCCTTGGTGTTCGCATTCTAGTTCATCCAAAGTTGAGACTGCAGGAGTTTTTAAGCATATACAACATTACACAAGATTTTATAACTGCCACTGAAAAGATTGGTGGAAGGTTGGGATATAGCATTCGTGGAACTTTGCAGTCACAGGCCAAAGCTTTTGTTGATTTTCAACACGAATCTCGTATGACAAAAATAAAAGCAGTGCTTGATCAGGAAACATGGGTTGAAGTGGATGTTCCTGACGAGTTTCAAGCTATAGCTGAATCACTTTATTCTCAAGAACTGCTTTCTGCAAAAATTGATGATGCTCAGGGTAATATGGATCGAAGCTACACTGATGTGACTACAAATAATGATGATTCAAGCATTGTAGGGGGTGGATCACTAAATGCTCAACAGCACTCTGAGCTGACTGATTCAAGTGACATGACGGGGGGGAATACTGAACATGCAAAGCCTACTCCTGCGGACAAAATTGAAAAAAGTAAAGCTGATGTGTTAATTCCTACAACACAAATTAACAATACTAATGTGAAGGAACGTGGAAAATCAAGCTCTCAGACTTTGCAATACAAAGGCATTGGTTATCACATGATGTTGTCGGAGTACATTGACATGAATGATTCTCTTCCAGCACTATCTTCTGAGGTCGTTCATCGTGTTGTGGAAATTCTCAAGTTTTTCAATACAAGGACTTGTCAACTTGTTCTTGGAGCTGGTGCTATGCAGGTGTCTGGTTTGAAGTCCATCACATCCAAGCACTTGGCACTGGCCAGTCAAGTTATCAGTTTTACTTGCGCGATTATTCCTGAAATTAGGAGAATACTTTTTCTCAAGGTACCTGAGGCGCGAAAGACACTATTGATCTCAGAGATTGATCGAGTGGCTCAAGATTACAAAGTTCACCGAGATGAAATTCATACTAAGCTGGTCCAGATAATGAGGGAGAGGTTATTGGTTCATCTTCGTGGCCTACCTCAAATTGTTGAAAGTTGGAATAGACTCCAGGATTCTGACCCTCAGCCCAGCCAGTTTGCTCGATCCCTTACCAAGGAAGTTGGGTACCTTCAGCGTGTCCTATCTCGAACCTTACATGAGGCTGATGTTCAGGCAATATTTAAGCAAGTGGTCAAAATCTTCCATTTACAAATTTCTGAAGCATTTTCACGGTTAGACATAAGTACCCCTCAAGCAAAGGATAGGCTGCTTCGGGATGTTAAGCACATTCTTGGCTGCATAAGATCTTTGCCTTGTGATGATTTGAGTAAACCAGACACCCCAAACTGGGGGCAACTTGATGAATTCTTGGATCAAAGATTCGGATCTGAAGCTGGATAA

Protein sequence

MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAAWWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIGYHMMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDTPNWGQLDEFLDQRFGSEAG
Homology
BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match: F4JT76 (Vacuolar protein sorting-associated protein 54, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VPS54 PE=1 SV=1)

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 670/1113 (60.20%), Postives = 807/1113 (72.51%), Query Frame = 0

Query: 1    MESQPSQSGRSPT---EYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGK- 60
            M+S PS  GRS T     S  L R +S   ++ S   K+ SDASSQSLSSILNNPH GK 
Sbjct: 1    MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60

Query: 61   ----SDASWAAWWSSSSTVSPPEFLPLSST-IASSEVTRFDFNNYIALISDSYHRFEDIR 120
                SDASW  WWSSS+ V+P EF P++ST +  SE+TR DF+ Y++ IS+S+ RFEDIR
Sbjct: 61   GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120

Query: 121  NHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQ 180
             H+ +ES G D        L ACLREVP+LYFKEDFALEDGATFR+ACPF ++ +NL LQ
Sbjct: 121  KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180

Query: 181  EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDL 240
            EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR+LKETIRLLD +L
Sbjct: 181  EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240

Query: 241  VDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLL 300
            VDSAR+IQE ++TR N+L LQ+KL+LILYVNQA+SALKLLVAS DCAGALD+TDDL +LL
Sbjct: 241  VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300

Query: 301  EGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLM 360
             GDEL+GL+CFRHLRDHV +SI+SI SIL++EFMR S HD G++D++I+     R S   
Sbjct: 301  AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360

Query: 361  NGK--DEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAE 420
            NG   +EVK +EE+ S   DRLLP++IGLLRTAK PS+LR+YRD +T++MK AIK AVA+
Sbjct: 361  NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420

Query: 421  LLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRA 480
            LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AIFKIVQ HLVRA
Sbjct: 421  LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480

Query: 481  AEVKKSIEWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVT 540
            +EVKK+IEWI+CN+DGHYAADSVAAAIA+GA AA TAQ+   Q G L+     +  +K  
Sbjct: 481  SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540

Query: 541  SLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVH 600
             L GK++DA++  NMSRNFR                                        
Sbjct: 541  PLQGKSSDASSLMNMSRNFR---------------------------------------- 600

Query: 601  IILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKL 660
                                               ADVLRENTEAVFAAC+  HGRWAKL
Sbjct: 601  -----------------------------------ADVLRENTEAVFAACEVTHGRWAKL 660

Query: 661  LGVRILVHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESR 720
            LGVR L+HPKL+LQEF+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESR
Sbjct: 661  LGVRALLHPKLKLQEFMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESR 720

Query: 721  MTKIKAVLDQETWVEVDVPDEFQAIAESLY-SQELLSAKIDDAQGNMDRSYTDVTTNNDD 780
            MTK+KAVLDQETW E+DVP+EFQ+I  SL+ SQ L+S K+DDA  N   SY       + 
Sbjct: 721  MTKLKAVLDQETWDEIDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNG 780

Query: 781  SSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPA-DKIEKSKADVLIPTTQINNTNVK 840
            S   G G  N++  +E ++SS+ +  +    KPT + + +E+SKA V   T   N +N K
Sbjct: 781  SLTSGSGDQNSELRNEKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQK 840

Query: 841  ERGKSSSQTLQYKGIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNT 900
              GKS+   L Y+G+GYHM         MLSEYIDMN+SLPALSSE+V RVVE+L+FFNT
Sbjct: 841  AHGKSN---LFYQGVGYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNT 900

Query: 901  RTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLIS 960
            RTCQLVLGAGAMQVSGLKSI +KHLALASQVI FT  IIPE RRILF KVPE RK LL  
Sbjct: 901  RTCQLVLGAGAMQVSGLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSV 960

Query: 961  EIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLT 1020
            EID+VAQD+++HRDEI+TKLVQIMRERLL HL GLP++VE WNR  D++ Q  +FA  LT
Sbjct: 961  EIDKVAQDFRIHRDEIYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLT 1020

Query: 1021 KEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHIL 1080
            +EVGYL RVLS TLHEADVQAIF+QV+ I H Q S+  + L+IS+ +AK RL   V+ IL
Sbjct: 1021 REVGYLHRVLSETLHEADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELIL 1028

Query: 1081 GCIRSLPCDDLSKPDTPNWGQLDEFLDQRFGSE 1089
             CIRSLP D+ ++ D PNWGQLDEF  + F  E
Sbjct: 1081 KCIRSLPSDNANQSDIPNWGQLDEFFAEHFREE 1028

BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match: Q9JMK8 (Vacuolar protein sorting-associated protein 54 OS=Rattus norvegicus OX=10116 GN=Vps54 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 3.0e-58
Identity = 239/929 (25.73%), Postives = 395/929 (42.52%), Query Frame = 0

Query: 132  LREVPALYFKEDFALEDGATFRAACPF-----------LNVTQNLVLQEKLSHYLDVVEL 191
            L +VP ++ K DFAL+D  TF +  P+            +   + +LQEKLSHYLD+VE+
Sbjct: 147  LEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEV 206

Query: 192  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 251
            ++  +ISLRS +FF A     +L   + +    ++ L++ I  +D  + + + +I     
Sbjct: 207  NIAHQISLRSEAFFHAMTSQHELQDYLKKTSQAVKMLRDKIAQIDKVMCEGSLQILRLAL 266

Query: 252  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 311
            TRNN + +  KLKL+  V+Q    +++L+++ +  GALD+      +L+  EL G+H FR
Sbjct: 267  TRNNCVKVSNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQ-QELQGVHSFR 326

Query: 312  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 371
            HL   +    + I  ++ AEF   S  D                +  + G+ +V   EEE
Sbjct: 327  HLGSQLCELEKLIDKMMIAEFSTYSHSD---------------LNRPLEGECQVL--EEE 386

Query: 372  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 431
                  RL+ ++ GLL   KL + L +Y +      K  IK  V  +  V  +  +D+D 
Sbjct: 387  ------RLVSLVFGLLEQRKL-NFLEIYGEETIITAKNIIKECV--INKVAQVEEIDTDA 446

Query: 432  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCN- 491
                           LA ++R L+   ++ LL  IF    + L R       I  ++ + 
Sbjct: 447  V------------VKLADQMRMLNFPQWIDLLKDIFSKFTIFLQRVKATLNVIHSVVLSV 506

Query: 492  LDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPS 551
            LD +     +         +AG     D +V  L     + +         +   AA  +
Sbjct: 507  LDKNQRTRELEEVSQ--QRSAGKDNSLDTEVAYL---THEGLFISDAFSEAEPASAAVDT 566

Query: 552  NMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLA 611
               RN           T P S     E C         S           D         
Sbjct: 567  TSQRN-----------TSPHS-----EPC---------SSDSVSEPECTTDSSSSKEQTP 626

Query: 612  LSFPLSAIILVEKEEMFERWKKADVLRENTEAVFA-ACDAAHGRWAKLLGVRIL--VHPK 671
             S  L  + ++  E+M     +   L  N + +   A D  H R  K L  R       K
Sbjct: 627  ASATLGGVDIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEK 686

Query: 672  LRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQ 731
            L   EF+++  + + +I  TE+I GR   S+ G LQSQA  FV+  HE R TK+  +LD 
Sbjct: 687  LNSTEFIALSRLMETYIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDN 746

Query: 732  ETWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNA 791
            E W + DVP EFQ + +S+            A G +                        
Sbjct: 747  ERWKQADVPAEFQDLVDSI------------ADGKI------------------------ 806

Query: 792  QQHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQY 851
                 L D               PA   ++  ADVL+                  +  QY
Sbjct: 807  ----ALPDKK-------------PAATEDRKPADVLV-----------------VEGHQY 866

Query: 852  KGIGYHMML----SEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGL 911
              +G  ++L     EY    D++P+++++++ R+ ++LK+FN+R+CQLVLGAGA+QV GL
Sbjct: 867  AVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRSCQLVLGAGALQVVGL 922

Query: 912  KSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEIH 971
            K+IT+K+LAL+S+ +      IP IR     ++P  + +LL    D + +DY  H  EI 
Sbjct: 927  KTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPRKQWSLL-RHFDHITKDYHDHIGEIS 922

Query: 972  TKLVQIMR---ERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTL 1031
            +KL+ IM    ++LL             +R +   P PS   R++ K++  +   +   L
Sbjct: 987  SKLIAIMDSLFDKLL-------------SRCEVEAPAPSPCFRNICKQMTKMHEAIFDLL 922

Query: 1032 HEADVQAIFKQVVKIFHLQISEAFSRLDI 1039
             +   Q +  ++   +   + +  S L++
Sbjct: 1047 PKEQTQMLILRINASYKFHLKKQLSHLNV 922

BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match: Q9P1Q0 (Vacuolar protein sorting-associated protein 54 OS=Homo sapiens OX=9606 GN=VPS54 PE=1 SV=2)

HSP 1 Score: 228.0 bits (580), Expect = 5.1e-58
Identity = 260/1026 (25.34%), Postives = 430/1026 (41.91%), Query Frame = 0

Query: 38   SSQSLSSILNNPHAGK--SDASWAAWWSS--SSTVSPPEFLPLSSTIASSEVTRFDFNNY 97
            S  +L + LN+P   K  SD     W      + V P  +LP        ++++  F  Y
Sbjct: 69   SKVNLPAALNDPRLAKRESDFFTKTWGLDFVDTEVIPSFYLP--------QISKEHFTVY 128

Query: 98   IALISDSYHRFEDIRNHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFR 157
               IS      E  +N    +     ++    +     L +VP ++ K DFAL+D  TF 
Sbjct: 129  QQEISQREKIHERCKNICPPKDTFERTLLHTHDKSRTDLEQVPKIFMKPDFALDDSLTFN 188

Query: 158  AACPF-----------LNVTQNLVLQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQD 217
            +  P+            +   + +LQEKLSHYLD+VE+++  +ISLRS +FF A     +
Sbjct: 189  SVLPWSHFNTAGGKGNRDAASSKLLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHE 248

Query: 218  LNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNATRNNLLALQQKLKLILYVNQAI 277
            L   + +    ++ L++ I  +D  + + +  I     TRNN + +  KLKL+  V+Q  
Sbjct: 249  LQDYLRKTSQAVKMLRDKIAQIDKVMCEGSLHILRLALTRNNCVKVYNKLKLMATVHQTQ 308

Query: 278  SALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFRHLRDHVAASIESITSILSAEFM 337
              +++L+++ +  GALD+      +L+  EL G+H FRHL   +    + I  ++ AEF 
Sbjct: 309  PTVQVLLSTSEFVGALDLIATTQEVLQ-QELQGIHSFRHLGSQLCELEKLIDKMMIAEF- 368

Query: 338  RASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEEASNFRDRLLPIIIGLLRTAKLP 397
              S +   D++  +              +D+ +  EEE      RL+ ++ GLL+  KL 
Sbjct: 369  --STYSHSDLNRPL--------------EDDCQVLEEE------RLISLVFGLLKQRKL- 428

Query: 398  SVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRG 457
            + L +Y + +    K  IK  V  +  V     +D+D                LA ++R 
Sbjct: 429  NFLEIYGEKMVITAKNIIKQCV--INKVSQTEEIDTDVV------------VKLADQMRM 488

Query: 458  LSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCN-LDGHYAADSVAAAIAIGAAAAG 517
            L+   +  LL  IF    + L R       I  ++ + LD +     +        AA  
Sbjct: 489  LNFPQWFDLLKDIFSKFTIFLQRVKATLNIIHSVVLSVLDKNQRTRELEEISQQKNAAKD 548

Query: 518  TAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSF 577
             + D++    L+   +    A     L   A D  +  N S N   C             
Sbjct: 549  NSLDTE-VAYLIHEGMFISDAFGEGELTPIAVDTTSQRNASPNSEPC------------- 608

Query: 578  GGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLALSFPLSAIILVEKEEMFERWKK 637
                           SS  +               T + + P    I+V ++      + 
Sbjct: 609  ---------------SSDSVSEPECTTDSSSSKEHTSSSAIPGGVDIMVSEDMKLTDSEL 668

Query: 638  ADVLRENTEAVFAACDAAHGRWAKLLGVRIL--VHPKLRLQEFLSIYNITQDFITATEKI 697
              +     E +++A D  H R  K L  R       KL   EF+++  + + FI  TE+I
Sbjct: 669  GKLANNIQELLYSASDICHDRAVKFLMSRAKDGFLEKLNSMEFITLSRLMETFILDTEQI 728

Query: 698  GGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQAIAESLYSQE 757
             GR   S+ G LQSQA  FV+  HE R TK+  +LD E W + DVP EFQ + +SL    
Sbjct: 729  CGRKSTSLLGALQSQAIKFVNRFHEERKTKLSLLLDNERWKQADVPAEFQDLVDSL---- 788

Query: 758  LLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPT 817
                                     D  I                  +   G TE  KP 
Sbjct: 789  ------------------------SDGKIA---------------LPEKKSGATEERKP- 848

Query: 818  PADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIGYHMML----SEYIDMNDSL 877
                     A+VLI   Q                 QY  +G  ++L     EY    D++
Sbjct: 849  ---------AEVLIVEGQ-----------------QYAVVGTVLLLIRIILEYCQCVDNI 908

Query: 878  PALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIP 937
            P+++++++ R+ ++LK+FN+R+CQLVLGAGA+QV GLK+IT+K+LAL+S+ +      IP
Sbjct: 909  PSVTTDMLTRLSDLLKYFNSRSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIP 934

Query: 938  EIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEIHTKLVQIMR---ERLLVHLRGLPQ 997
             IR     ++P  + ++L    D + +DY  H  EI  KLV IM    ++LL        
Sbjct: 969  VIRAHFEARLPPKQYSML-RHFDHITKDYHDHIAEISAKLVAIMDSLFDKLL-------- 934

Query: 998  IVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEA 1039
                 ++ +   P PS   R++ K++  +   +   L E   Q +F ++   + L + + 
Sbjct: 1029 -----SKYEVKAPVPSACFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQ 934

BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match: Q5SPW0 (Vacuolar protein sorting-associated protein 54 OS=Mus musculus OX=10090 GN=Vps54 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 1.4e-55
Identity = 268/1064 (25.19%), Postives = 444/1064 (41.73%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGK--SDASW 60
            ++S P    + PT  S  L    SL  T   +     S  +L + LN+P   K  SD   
Sbjct: 33   VQSLPDVCPKEPTGDSHTLCVAPSL-VTDQHRWTVYHSKVNLPAALNDPTLAKRESDFFT 92

Query: 61   AAWWSS--SSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESV 120
              W      + V P  +LP        ++++ +F  Y   IS      E  +N    +  
Sbjct: 93   KTWGLDFVDTEVIPSLYLP--------QISKENFIAYQQEISQREKIHERCKNICPPKDT 152

Query: 121  GLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPF-----------LNVTQNL 180
               ++    +     L +VP ++ K DFAL+D  TF +  P+            +   + 
Sbjct: 153  FDRTLLHIHDKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSK 212

Query: 181  VLQEKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLD 240
            +LQEKLSHYLD+VE+++  +ISLRS +FF A     +L   + +    ++ L++ I  +D
Sbjct: 213  LLQEKLSHYLDIVEVNIAHQISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQID 272

Query: 241  VDLVDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLL 300
              + + + +I     TRNN + +  KLKL+  V+Q    +++L+++ +  GALD+     
Sbjct: 273  KVMCEGSLQILRLALTRNNCVKVYNKLKLMATVHQTQPTVQVLLSTSEFVGALDLIATTQ 332

Query: 301  HLLEGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARAS 360
             +L+  EL G+H FRHL   +    + I  ++ AEF   S  D                +
Sbjct: 333  EVLQ-QELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYSHSD---------------LN 392

Query: 361  NLMNGKDEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVA 420
              + G+ +V   EEE      RL+ ++ GLL+  KL + L +Y + +    K  IK  V 
Sbjct: 393  RPLEGECQVL--EEE------RLVSLVFGLLKQRKL-NFLEIYGEEMIITAKNIIKERV- 452

Query: 421  ELLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVR 480
             +  V  +  +D+D                LA ++R L+   ++ LL  IF    V L R
Sbjct: 453  -INKVSQIEEIDTDVV------------VKLADQMRMLNFPQWIDLLKDIFSKFTVFLQR 512

Query: 481  AAEVKKSIEWIMCN-LDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAK 540
                   I  ++ + L+       +       +A   ++ D+D      L H    ++  
Sbjct: 513  VKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDSSLDTD---VAYLTHEGWFISDA 572

Query: 541  VTSLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHS 600
             +   G+   AA  +   RN           T P S     E C         S      
Sbjct: 573  FSE--GEPASAAVDTTSQRN-----------TSPHS-----EPC---------SSDSVSE 632

Query: 601  VHIILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFA-ACDAAHGRW 660
                 D        +   P   I ++  E+M     +   L  N + +   A D  H R 
Sbjct: 633  PECTTDSSSSKEQTSACAPPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRA 692

Query: 661  AKLLGVRIL--VHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDF 720
             K L  R       KL   EF+++  + + FI  TE+I GR   S+ G LQSQA  FV+ 
Sbjct: 693  VKFLMSRAKDGFLEKLNSTEFIALSRLMETFIVDTEQICGRKSTSLLGALQSQANKFVNR 752

Query: 721  QHESRMTKIKAVLDQETWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTT 780
             HE R TK+  +LD E W + DVP EFQ + +S+                          
Sbjct: 753  FHEERRTKLSLLLDNERWKQADVPAEFQDLVDSI-------------------------- 812

Query: 781  NNDDSSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNT 840
                                         G     +  P    E+  ADVL+        
Sbjct: 813  ---------------------------ADGKIALPEKKPVVTEERKPADVLV-------- 872

Query: 841  NVKERGKSSSQTLQYKGIGYHMML----SEYIDMNDSLPALSSEVVHRVVEILKFFNTRT 900
                      +  QY  +G  ++L     EY    D++P+++++++ R+ ++LK+FN+R+
Sbjct: 873  ---------VEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNSRS 932

Query: 901  CQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEI 960
            CQLVLGAGA+QV GLK+IT+K+LAL+S+ +      IP IR     ++P  + ++L    
Sbjct: 933  CQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSML-RHF 934

Query: 961  DRVAQDYKVHRDEIHTKLVQIMR---ERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSL 1020
            D + +DY  H  EI  KLV IM    ++LL             ++ +   P PS   R++
Sbjct: 993  DHITKDYHDHIAEISAKLVAIMDSLFDKLL-------------SKYEVKAPVPSPCFRNI 934

Query: 1021 TKEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDI 1039
             K++  +   +   L E   Q +F ++   + L + +  S L++
Sbjct: 1053 CKQMTKMHEAIFDLLPEEQTQMLFLRINASYKLHLKKQLSHLNV 934

BLAST of MC01g1658 vs. ExPASy Swiss-Prot
Match: Q9VLC0 (Vacuolar protein sorting-associated protein 54 OS=Drosophila melanogaster OX=7227 GN=scat PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 9.0e-39
Identity = 224/1024 (21.88%), Postives = 383/1024 (37.40%), Query Frame = 0

Query: 37   ASSQSLSSILNNPHAGKSD----ASWAAWWSSSSTVSPPEFLPLSSTIASSEVTRFDFNN 96
            +++Q+L ++LN+P  GK        W   +   S V P   LP        ++T  DF  
Sbjct: 124  SAAQNLPAVLNDPSRGKQSNLFTKKWGEHFVERSHVPPSPRLP--------DITHADFTV 183

Query: 97   YIALISDSYHRFEDIRNHSTKESVGLDSIG------GQGEALVACLREVPALYFKEDFAL 156
            Y+  I   Y R+ + R    +    L++        G G      L  VP ++ K    L
Sbjct: 184  YLGSIGKRY-RWHERRQQQLERDKPLENGAQGAPGPGTGGQTPTHLSSVPEIFLKSQLQL 243

Query: 157  EDGATFR----------AACPFLNVTQNLVLQEKLSHYLDVVELHLVKEISLRSNSFFEA 216
               ATF+          A+ P  +      LQE+LSHYLD+VE+ + +++S +S +FF A
Sbjct: 244  HHPATFKQVFPNYMQTSASSPESHQQTGRQLQEQLSHYLDMVEVKIAQQVSQKSAAFFHA 303

Query: 217  QGQLQDLNVKIVEGCSRIRQLKETI-RLLDVDLVDSAREIQEQNATRNNLLALQQKLKLI 276
                  +  ++ +   ++RQL+  +  L    +VDS + ++     ++  L L  KL+L+
Sbjct: 304  MTTQHAILAEMEQAADQVRQLRAALAELHSHSVVDSFKVLRFAQRRQHYNLTL-DKLRLM 363

Query: 277  LYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFRHLRDHVAASIESITS 336
              V++    L+LL+ + D   ALD+      +L   EL G+HCF+HL   ++   + I  
Sbjct: 364  ATVHKTQPMLQLLLGTQDYVAALDLIGTTQEILSA-ELLGIHCFKHLPMQLSEMEKLIDK 423

Query: 337  ILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEEASNFRDRLLPIIIGL 396
            +L+ EF R   + A D++  +        ++ +   D V  +E       D+L+ I++GL
Sbjct: 424  MLTTEFER---YAAADLNRPL--------TDALRETDSVCAEE-------DKLVAIVMGL 483

Query: 397  LRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDFAPGERTVDADGGGAS 456
            LR     S ++ Y+    A ++  IK  + E+L                           
Sbjct: 484  LRKQNF-SFVQAYQQEAIATIRAIIKQLLIEVL--------------------------- 543

Query: 457  LASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNLDGHYAADSVAAAIAI 516
                                                                        
Sbjct: 544  ------------------------------------------------------------ 603

Query: 517  GAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSNMSRNFRYCFVVLIYF 576
                     DSD ++                SL G    A                 +  
Sbjct: 604  ------ARSDSDQEI----------------SLTGHGEQA-----------------LEL 663

Query: 577  TFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLALSFPLSAIILVEKEEM 636
            T P       E  A L R   +   +   +  ++ + +  +  A+     A+ L++ E  
Sbjct: 664  TLP-------EWIALLQRSSQALVSILERIKTVVGIMQQTADAAVG-AQDAVNLIDSEAF 723

Query: 637  FERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQEFLSIYNITQDFITA 696
                    +  +  + + A C   H R A ++  + L       QE   +  I   F   
Sbjct: 724  LSPGHHEQLKNQLQQLLQAVCHYCHERCANIVSPQSLERSSASEQELFQLSEIVDHFGET 783

Query: 697  TEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWVEVDVPDEFQAIAESL 756
            T  I G     ++  L+ QA  +    H  R  K+  +LDQE W +VD+P EFQ I E  
Sbjct: 784  TRSICGVASVPLQLALKVQASRYAQRFHSERKQKLSLLLDQERWRQVDIPHEFQRIIER- 843

Query: 757  YSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHSELTDSSDMTGGNTEH 816
                                                                M  G  ++
Sbjct: 844  ----------------------------------------------------MAAG--DY 897

Query: 817  AKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIGYHMMLSEYIDMNDSL 876
            AKP   + I               N  +   GK    TL    +    ML EY      L
Sbjct: 904  AKPEMGNLISNG----------AGNPVLLVEGK-QPYTLVSASLMLIRMLYEYGCSAHRL 897

Query: 877  PALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIP 936
            P L+S     VV++L+ FN+R+CQL++GAGAM+V+GLK+ITS +LAL S+ +     ++P
Sbjct: 964  PLLASYHARNVVDLLRCFNSRSCQLIIGAGAMRVAGLKTITSTNLALVSRALQLVLWLLP 897

Query: 937  EIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVE 996
            +++               +S  + + +DY+ H  EI  K+  I+ ERL   L       +
Sbjct: 1024 KLK----------EHFQAMSGYETIERDYQGHIKEIENKIHGIVSERLAAQL-------D 897

Query: 997  SWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSR 1040
            +W   +   P PSQ  R +++ +  L   ++  L EA +  I+  V + F  ++ E   +
Sbjct: 1084 AW---EARPPIPSQTFRHISRHLVKLHEAIAGVLPEAQIHEIYGVVHRNFKDKLREQLLK 897

BLAST of MC01g1658 vs. NCBI nr
Match: XP_022146560.1 (vacuolar protein sorting-associated protein 54, chloroplastic [Momordica charantia])

HSP 1 Score: 1898 bits (4917), Expect = 0.0
Identity = 1014/1099 (92.27%), Postives = 1014/1099 (92.27%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
            MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA
Sbjct: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60

Query: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
            WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNY ALISDSYHRFEDIRNHSTKESVGLDS
Sbjct: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYTALISDSYHRFEDIRNHSTKESVGLDS 120

Query: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
            IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL
Sbjct: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180

Query: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
            HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240

Query: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
            TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR
Sbjct: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300

Query: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
            HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE
Sbjct: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360

Query: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
            ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF
Sbjct: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420

Query: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
            APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL
Sbjct: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480

Query: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
            DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN
Sbjct: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540

Query: 541  MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
            MSRNFR                                                      
Sbjct: 541  MSRNFR------------------------------------------------------ 600

Query: 601  SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
                                 ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601  ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660

Query: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
            EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV
Sbjct: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720

Query: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
            EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS
Sbjct: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780

Query: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
            ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG
Sbjct: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840

Query: 841  YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
            YHM         MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841  YHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900

Query: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
            LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI
Sbjct: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960

Query: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
            HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020

Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
            ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT
Sbjct: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1024

Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
            PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1024

BLAST of MC01g1658 vs. NCBI nr
Match: XP_038882115.1 (vacuolar protein sorting-associated protein 54, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1769 bits (4581), Expect = 0.0
Identity = 946/1102 (85.84%), Postives = 979/1102 (88.84%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGKSDAS 60
            MESQPSQSGRSPT+YSTL+SR+TSL RTTSS   K+NSDASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MESQPSQSGRSPTDYSTLVSRQTSLGRTTSSSSAKSNSDASSQSLSSILNNPHAGKSDAS 60

Query: 61   WAAWWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVG 120
            W  WWSSSST+SPPEF+PL+STIA+SEVTRFDFNNY ALISDS++RFEDIRNHS+KE+ G
Sbjct: 61   WVGWWSSSSTISPPEFMPLASTIATSEVTRFDFNNYTALISDSFNRFEDIRNHSSKENGG 120

Query: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDV 180
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNV+QNLVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNLVLQEKLSHYLDV 180

Query: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQE 240
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLD DLVDSAREIQE
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDADLVDSAREIQE 240

Query: 241  QNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLH 300
            QNATRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLH
Sbjct: 241  QNATRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 301  CFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFD 360
            CFRHLRDHVAASIESITSILSAEFMRAS HDAGDVD+ II ETKA ASNLMNGKDEVK D
Sbjct: 301  CFRHLRDHVAASIESITSILSAEFMRASIHDAGDVDIGIITETKAWASNLMNGKDEVKLD 360

Query: 361  EEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLD 420
            EEE SNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLL+RPLD
Sbjct: 361  EEETSNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLIRPLD 420

Query: 421  SDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIM 480
            SDFAPGERT+DADGGG SLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIM
Sbjct: 421  SDFAPGERTMDADGGGVSLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIM 480

Query: 481  CNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAAN 540
            CNLDGHYAADSVAAAIA GAAAAGTAQD+DNQ GLLLPHLPQRVAAKVTSL GKANDAAN
Sbjct: 481  CNLDGHYAADSVAAAIATGAAAAGTAQDTDNQGGLLLPHLPQRVAAKVTSLQGKANDAAN 540

Query: 541  PSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLAST 600
            PSNMSRNFR                                                   
Sbjct: 541  PSNMSRNFR--------------------------------------------------- 600

Query: 601  LALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKL 660
                                    ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKL
Sbjct: 601  ------------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKL 660

Query: 661  RLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQE 720
            RLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQE
Sbjct: 661  RLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQE 720

Query: 721  TWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQ 780
            TWVEVDVPDEFQ+IAESL SQELLS K+D AQGNMD+SY+DV TNND S IVGGGSLNAQ
Sbjct: 721  TWVEVDVPDEFQSIAESLCSQELLSEKVDLAQGNMDQSYSDVATNNDASRIVGGGSLNAQ 780

Query: 781  QHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYK 840
             +SE  DSSDM+GGNTEH KPTPAD IEKS+ADV IPT QINN NVKERGKSSSQTL +K
Sbjct: 781  HYSEQIDSSDMSGGNTEHVKPTPADTIEKSRADVTIPTMQINNINVKERGKSSSQTLLHK 840

Query: 841  GIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQ 900
            G+GYHM         MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQ
Sbjct: 841  GVGYHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQ 900

Query: 901  VSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHR 960
            VSGLKSITSKHLALASQVISFT AIIPEIRRILF KVPE RKTLL+SEIDRVAQD+KVHR
Sbjct: 901  VSGLKSITSKHLALASQVISFTFAIIPEIRRILFFKVPEVRKTLLLSEIDRVAQDFKVHR 960

Query: 961  DEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRT 1020
            DEIHTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRT
Sbjct: 961  DEIHTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRT 1020

Query: 1021 LHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK 1080
            LHEADV+AIF++VVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK
Sbjct: 1021 LHEADVKAIFREVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK 1027

Query: 1081 PDTPNWGQLDEFLDQRFGSEAG 1090
            PD PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PDIPNWGQLDEFLDQRFGSEAG 1027

BLAST of MC01g1658 vs. NCBI nr
Match: XP_004134530.1 (vacuolar protein sorting-associated protein 54, chloroplastic [Cucumis sativus] >KGN49522.1 hypothetical protein Csa_003061 [Cucumis sativus])

HSP 1 Score: 1744 bits (4517), Expect = 0.0
Identity = 936/1099 (85.17%), Postives = 970/1099 (88.26%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
            M+SQPSQSGRSPT+YST+++R+TSL RTTSS   SDASSQSLSSILNNPHAGKSDASW  
Sbjct: 1    MDSQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60

Query: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
            WWSSSSTV+PPEF+PLSSTIASSEVTRFDFNNY ALISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61   WWSSSSTVNPPEFMPLSSTIASSEVTRFDFNNYTALISDSFHRFEDIRNHSSKENGGLDS 120

Query: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
            IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121  IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180

Query: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
            HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240

Query: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
            TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300

Query: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
            HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301  HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360

Query: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
             SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLL+RP DSDF
Sbjct: 361  TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLIRPHDSDF 420

Query: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
            APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHLVRAAEVKKSIEWIMCNL
Sbjct: 421  APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLVRAAEVKKSIEWIMCNL 480

Query: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
            DGHYAADSVAAAIA GAAAAGTAQD+DNQ GLLLPHLPQRVAAKV SL GKANDAANPSN
Sbjct: 481  DGHYAADSVAAAIASGAAAAGTAQDTDNQGGLLLPHLPQRVAAKVISLQGKANDAANPSN 540

Query: 541  MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
            MSRNFR                                                      
Sbjct: 541  MSRNFR------------------------------------------------------ 600

Query: 601  SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
                                 ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601  ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660

Query: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
            EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVD+QHESRMTKIKAVLDQETWV
Sbjct: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDYQHESRMTKIKAVLDQETWV 720

Query: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
            EVDVPDEFQ+IAESL SQELLS K D  Q NMDRSY DV TNNDDS        NAQQHS
Sbjct: 721  EVDVPDEFQSIAESLCSQELLSEKPDLTQDNMDRSYGDVATNNDDSH-------NAQQHS 780

Query: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
            E  DSSD++GGN+EH KPTPAD  EKSKADV IPT Q++NTNVKERGKSSSQTL YKG+G
Sbjct: 781  EQIDSSDLSGGNSEHVKPTPADTTEKSKADVTIPTMQVSNTNVKERGKSSSQTLLYKGVG 840

Query: 841  YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
            YHM         MLSEYIDMN+SLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841  YHMVNCGLILLKMLSEYIDMNNSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900

Query: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
            LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901  LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960

Query: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
            HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014

Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
            ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPD 
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDI 1014

Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
            PNWGQLDEFL+QRFGSEAG
Sbjct: 1081 PNWGQLDEFLEQRFGSEAG 1014

BLAST of MC01g1658 vs. NCBI nr
Match: KAA0052432.1 (vacuolar protein sorting-associated protein 54 [Cucumis melo var. makuwa])

HSP 1 Score: 1735 bits (4493), Expect = 0.0
Identity = 931/1099 (84.71%), Postives = 971/1099 (88.35%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
            MESQPSQSGRSPT+YST+++R+TSL RTTSS   SDASSQSLSSILNNPHAGKSDASW  
Sbjct: 1    MESQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60

Query: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
            WWSSSSTV+PPEF+PLSS+IASSEVTRFDFNNY  LISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61   WWSSSSTVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDS 120

Query: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
            IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121  IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180

Query: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
            HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240

Query: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
            TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300

Query: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
            HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301  HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360

Query: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
             SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELL VLL+RPLDSDF
Sbjct: 361  TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLHVLLIRPLDSDF 420

Query: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
            APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHL+RAAEVKKSIEWIMCNL
Sbjct: 421  APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLMRAAEVKKSIEWIMCNL 480

Query: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
            DGHYAADSVAAAIA GAAAAGTAQD+D+Q GLLLPHLPQRVAAKVTSL GKANDAANPSN
Sbjct: 481  DGHYAADSVAAAIASGAAAAGTAQDTDSQGGLLLPHLPQRVAAKVTSLQGKANDAANPSN 540

Query: 541  MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
            MSRNFR                                                      
Sbjct: 541  MSRNFR------------------------------------------------------ 600

Query: 601  SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
                                 ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601  ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660

Query: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
            EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWV
Sbjct: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWV 720

Query: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
            EVDVPDEFQ+IAESL SQELLS   D AQGNMD+S++DV T+NDDS        NAQ+HS
Sbjct: 721  EVDVPDEFQSIAESLCSQELLSENPDLAQGNMDQSHSDVATDNDDS-------RNAQEHS 780

Query: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
            +  DSSD++GGN+EH K TPAD IEKSKADV IP+TQ+NNTNVKERGKSSSQTL YKG+G
Sbjct: 781  QQIDSSDLSGGNSEHVKLTPADTIEKSKADVTIPSTQLNNTNVKERGKSSSQTLLYKGVG 840

Query: 841  YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
            YHM         MLSEYIDMN+SLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841  YHMVNCGLILLKMLSEYIDMNNSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900

Query: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
            LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901  LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960

Query: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
            HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014

Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
            ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK D 
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKSDI 1014

Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
            PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1014

BLAST of MC01g1658 vs. NCBI nr
Match: XP_008439467.1 (PREDICTED: vacuolar protein sorting-associated protein 54, chloroplastic [Cucumis melo])

HSP 1 Score: 1733 bits (4489), Expect = 0.0
Identity = 930/1099 (84.62%), Postives = 970/1099 (88.26%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
            MESQPSQSGRSPT+YST+++R+TSL RTTSS   SDASSQSLSSILNNPHAGKSDASW  
Sbjct: 1    MESQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60

Query: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
            WWSSSSTV+PPEF+PLSS+IASSEVTRFDFNNY  LISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61   WWSSSSTVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDS 120

Query: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
            IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121  IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180

Query: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
            HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240

Query: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
            TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300

Query: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
            HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301  HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360

Query: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
             SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELL VLL+RPLDSDF
Sbjct: 361  TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLHVLLIRPLDSDF 420

Query: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
            APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHL+RAAEVKKSIEWIMCNL
Sbjct: 421  APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLMRAAEVKKSIEWIMCNL 480

Query: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
            DGHYAADSVAAAIA GAAAAGTAQD+D+Q GLLLPHLPQRVAAKVTSL GKANDAANPSN
Sbjct: 481  DGHYAADSVAAAIASGAAAAGTAQDTDSQGGLLLPHLPQRVAAKVTSLQGKANDAANPSN 540

Query: 541  MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
            MSRNFR                                                      
Sbjct: 541  MSRNFR------------------------------------------------------ 600

Query: 601  SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
                                 ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601  ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660

Query: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
            EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWV
Sbjct: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWV 720

Query: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
            EVDVPDEFQ+IAESL SQELLS   D AQGNMD+S++DV T+NDDS        NAQ+HS
Sbjct: 721  EVDVPDEFQSIAESLCSQELLSENPDLAQGNMDQSHSDVATDNDDS-------RNAQEHS 780

Query: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
            +  DSSD++GGN+EH K TPAD IEKSKADV IP+TQ+NNTNVKERGKSSSQTL YKG+G
Sbjct: 781  QQIDSSDLSGGNSEHVKLTPADTIEKSKADVTIPSTQLNNTNVKERGKSSSQTLLYKGVG 840

Query: 841  YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
            YHM         MLSEYIDMN+S PALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841  YHMVNCGLILLKMLSEYIDMNNSFPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900

Query: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
            LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901  LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960

Query: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
            HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014

Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
            ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK D 
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKSDI 1014

Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
            PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1014

BLAST of MC01g1658 vs. ExPASy TrEMBL
Match: A0A6J1CXK3 (vacuolar protein sorting-associated protein 54, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015746 PE=3 SV=1)

HSP 1 Score: 1898 bits (4917), Expect = 0.0
Identity = 1014/1099 (92.27%), Postives = 1014/1099 (92.27%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
            MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA
Sbjct: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60

Query: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
            WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNY ALISDSYHRFEDIRNHSTKESVGLDS
Sbjct: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYTALISDSYHRFEDIRNHSTKESVGLDS 120

Query: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
            IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL
Sbjct: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180

Query: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
            HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240

Query: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
            TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR
Sbjct: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300

Query: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
            HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE
Sbjct: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360

Query: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
            ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF
Sbjct: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420

Query: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
            APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL
Sbjct: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480

Query: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
            DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN
Sbjct: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540

Query: 541  MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
            MSRNFR                                                      
Sbjct: 541  MSRNFR------------------------------------------------------ 600

Query: 601  SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
                                 ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601  ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660

Query: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
            EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV
Sbjct: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720

Query: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
            EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS
Sbjct: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780

Query: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
            ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG
Sbjct: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840

Query: 841  YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
            YHM         MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841  YHMVNCGLILLKMLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900

Query: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
            LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI
Sbjct: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960

Query: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
            HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020

Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
            ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT
Sbjct: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1024

Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
            PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1024

BLAST of MC01g1658 vs. ExPASy TrEMBL
Match: A0A0A0KMC9 (Vps54 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G526560 PE=3 SV=1)

HSP 1 Score: 1744 bits (4517), Expect = 0.0
Identity = 936/1099 (85.17%), Postives = 970/1099 (88.26%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
            M+SQPSQSGRSPT+YST+++R+TSL RTTSS   SDASSQSLSSILNNPHAGKSDASW  
Sbjct: 1    MDSQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60

Query: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
            WWSSSSTV+PPEF+PLSSTIASSEVTRFDFNNY ALISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61   WWSSSSTVNPPEFMPLSSTIASSEVTRFDFNNYTALISDSFHRFEDIRNHSSKENGGLDS 120

Query: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
            IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121  IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180

Query: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
            HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240

Query: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
            TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300

Query: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
            HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301  HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360

Query: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
             SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLL+RP DSDF
Sbjct: 361  TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLIRPHDSDF 420

Query: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
            APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHLVRAAEVKKSIEWIMCNL
Sbjct: 421  APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLVRAAEVKKSIEWIMCNL 480

Query: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
            DGHYAADSVAAAIA GAAAAGTAQD+DNQ GLLLPHLPQRVAAKV SL GKANDAANPSN
Sbjct: 481  DGHYAADSVAAAIASGAAAAGTAQDTDNQGGLLLPHLPQRVAAKVISLQGKANDAANPSN 540

Query: 541  MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
            MSRNFR                                                      
Sbjct: 541  MSRNFR------------------------------------------------------ 600

Query: 601  SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
                                 ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601  ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660

Query: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
            EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVD+QHESRMTKIKAVLDQETWV
Sbjct: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDYQHESRMTKIKAVLDQETWV 720

Query: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
            EVDVPDEFQ+IAESL SQELLS K D  Q NMDRSY DV TNNDDS        NAQQHS
Sbjct: 721  EVDVPDEFQSIAESLCSQELLSEKPDLTQDNMDRSYGDVATNNDDSH-------NAQQHS 780

Query: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
            E  DSSD++GGN+EH KPTPAD  EKSKADV IPT Q++NTNVKERGKSSSQTL YKG+G
Sbjct: 781  EQIDSSDLSGGNSEHVKPTPADTTEKSKADVTIPTMQVSNTNVKERGKSSSQTLLYKGVG 840

Query: 841  YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
            YHM         MLSEYIDMN+SLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841  YHMVNCGLILLKMLSEYIDMNNSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900

Query: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
            LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901  LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960

Query: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
            HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014

Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
            ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPD 
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDI 1014

Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
            PNWGQLDEFL+QRFGSEAG
Sbjct: 1081 PNWGQLDEFLEQRFGSEAG 1014

BLAST of MC01g1658 vs. ExPASy TrEMBL
Match: A0A5A7U9K3 (Vacuolar protein sorting-associated protein 54 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00290 PE=3 SV=1)

HSP 1 Score: 1735 bits (4493), Expect = 0.0
Identity = 931/1099 (84.71%), Postives = 971/1099 (88.35%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
            MESQPSQSGRSPT+YST+++R+TSL RTTSS   SDASSQSLSSILNNPHAGKSDASW  
Sbjct: 1    MESQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60

Query: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
            WWSSSSTV+PPEF+PLSS+IASSEVTRFDFNNY  LISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61   WWSSSSTVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDS 120

Query: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
            IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121  IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180

Query: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
            HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240

Query: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
            TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300

Query: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
            HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301  HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360

Query: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
             SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELL VLL+RPLDSDF
Sbjct: 361  TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLHVLLIRPLDSDF 420

Query: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
            APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHL+RAAEVKKSIEWIMCNL
Sbjct: 421  APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLMRAAEVKKSIEWIMCNL 480

Query: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
            DGHYAADSVAAAIA GAAAAGTAQD+D+Q GLLLPHLPQRVAAKVTSL GKANDAANPSN
Sbjct: 481  DGHYAADSVAAAIASGAAAAGTAQDTDSQGGLLLPHLPQRVAAKVTSLQGKANDAANPSN 540

Query: 541  MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
            MSRNFR                                                      
Sbjct: 541  MSRNFR------------------------------------------------------ 600

Query: 601  SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
                                 ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601  ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660

Query: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
            EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWV
Sbjct: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWV 720

Query: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
            EVDVPDEFQ+IAESL SQELLS   D AQGNMD+S++DV T+NDDS        NAQ+HS
Sbjct: 721  EVDVPDEFQSIAESLCSQELLSENPDLAQGNMDQSHSDVATDNDDS-------RNAQEHS 780

Query: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
            +  DSSD++GGN+EH K TPAD IEKSKADV IP+TQ+NNTNVKERGKSSSQTL YKG+G
Sbjct: 781  QQIDSSDLSGGNSEHVKLTPADTIEKSKADVTIPSTQLNNTNVKERGKSSSQTLLYKGVG 840

Query: 841  YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
            YHM         MLSEYIDMN+SLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841  YHMVNCGLILLKMLSEYIDMNNSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900

Query: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
            LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901  LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960

Query: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
            HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014

Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
            ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK D 
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKSDI 1014

Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
            PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1014

BLAST of MC01g1658 vs. ExPASy TrEMBL
Match: A0A1S3AYS9 (vacuolar protein sorting-associated protein 54, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484260 PE=3 SV=1)

HSP 1 Score: 1733 bits (4489), Expect = 0.0
Identity = 930/1099 (84.62%), Postives = 970/1099 (88.26%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSSKTNSDASSQSLSSILNNPHAGKSDASWAA 60
            MESQPSQSGRSPT+YST+++R+TSL RTTSS   SDASSQSLSSILNNPHAGKSDASW  
Sbjct: 1    MESQPSQSGRSPTDYSTIVTRQTSLGRTTSS---SDASSQSLSSILNNPHAGKSDASWVG 60

Query: 61   WWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVGLDS 120
            WWSSSSTV+PPEF+PLSS+IASSEVTRFDFNNY  LISDS+HRFEDIRNHS+KE+ GLDS
Sbjct: 61   WWSSSSTVNPPEFMPLSSSIASSEVTRFDFNNYTTLISDSFHRFEDIRNHSSKENGGLDS 120

Query: 121  IGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDVVEL 180
            IGGQGEALVACLREVPALYFKEDFALE+GATFRAACPFLNV+QNLVLQEKLSHYLDVVEL
Sbjct: 121  IGGQGEALVACLREVPALYFKEDFALEEGATFRAACPFLNVSQNLVLQEKLSHYLDVVEL 180

Query: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240
            HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA
Sbjct: 181  HLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQEQNA 240

Query: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLHCFR 300
            TRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLHCFR
Sbjct: 241  TRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLHCFR 300

Query: 301  HLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKFDEEE 360
            HLRDHVAASIESITSILSAEFMRAS HDAGDVD+VII ETKA ASNLMNGKDEVK DEEE
Sbjct: 301  HLRDHVAASIESITSILSAEFMRASIHDAGDVDIVIITETKAWASNLMNGKDEVKLDEEE 360

Query: 361  ASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLMRPLDSDF 420
             SNFRDRLLPI+IGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELL VLL+RPLDSDF
Sbjct: 361  TSNFRDRLLPIVIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLHVLLIRPLDSDF 420

Query: 421  APGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSIEWIMCNL 480
            APGERT+DADGGGASLASKLRGLSSEGFVQLLSAIFKIV+VHL+RAAEVKKSIEWIMCNL
Sbjct: 421  APGERTMDADGGGASLASKLRGLSSEGFVQLLSAIFKIVRVHLMRAAEVKKSIEWIMCNL 480

Query: 481  DGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKANDAANPSN 540
            DGHYAADSVAAAIA GAAAAGTAQD+D+Q GLLLPHLPQRVAAKVTSL GKANDAANPSN
Sbjct: 481  DGHYAADSVAAAIASGAAAAGTAQDTDSQGGLLLPHLPQRVAAKVTSLQGKANDAANPSN 540

Query: 541  MSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQKLASTLAL 600
            MSRNFR                                                      
Sbjct: 541  MSRNFR------------------------------------------------------ 600

Query: 601  SFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660
                                 ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ
Sbjct: 601  ---------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILVHPKLRLQ 660

Query: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAVLDQETWV 720
            EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRM KIKAVLDQETWV
Sbjct: 661  EFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMAKIKAVLDQETWV 720

Query: 721  EVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGSLNAQQHS 780
            EVDVPDEFQ+IAESL SQELLS   D AQGNMD+S++DV T+NDDS        NAQ+HS
Sbjct: 721  EVDVPDEFQSIAESLCSQELLSENPDLAQGNMDQSHSDVATDNDDS-------RNAQEHS 780

Query: 781  ELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQTLQYKGIG 840
            +  DSSD++GGN+EH K TPAD IEKSKADV IP+TQ+NNTNVKERGKSSSQTL YKG+G
Sbjct: 781  QQIDSSDLSGGNSEHVKLTPADTIEKSKADVTIPSTQLNNTNVKERGKSSSQTLLYKGVG 840

Query: 841  YHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900
            YHM         MLSEYIDMN+S PALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG
Sbjct: 841  YHMVNCGLILLKMLSEYIDMNNSFPALSSEVVHRVVEILKFFNTRTCQLVLGAGAMQVSG 900

Query: 901  LKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDYKVHRDEI 960
            LKSITSKHLALASQVISFT AIIPEIRRILFLKVPEARKTLL+SEIDRVAQD+KVHRDEI
Sbjct: 901  LKSITSKHLALASQVISFTFAIIPEIRRILFLKVPEARKTLLLSEIDRVAQDFKVHRDEI 960

Query: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1020
            HTKLVQIMRERLLVHLRGLPQIVESWNRL+DSDPQPSQFARSLTKEVGYLQRVLSRTLHE
Sbjct: 961  HTKLVQIMRERLLVHLRGLPQIVESWNRLEDSDPQPSQFARSLTKEVGYLQRVLSRTLHE 1014

Query: 1021 ADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKPDT 1080
            ADVQAIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSK D 
Sbjct: 1021 ADVQAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCDDLSKSDI 1014

Query: 1081 PNWGQLDEFLDQRFGSEAG 1090
            PNWGQLDEFLDQRFGSEAG
Sbjct: 1081 PNWGQLDEFLDQRFGSEAG 1014

BLAST of MC01g1658 vs. ExPASy TrEMBL
Match: A0A6J1EEU2 (vacuolar protein sorting-associated protein 54, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433683 PE=3 SV=1)

HSP 1 Score: 1722 bits (4459), Expect = 0.0
Identity = 928/1106 (83.91%), Postives = 967/1106 (87.43%), Query Frame = 0

Query: 1    MESQPSQSGRSPTEYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGKSDAS 60
            M+SQPSQSGRSPTEYS+LLSRET+L RTTSS   K+N DASSQSLSSILNNPHAGKSDAS
Sbjct: 1    MDSQPSQSGRSPTEYSSLLSRETTLGRTTSSSSPKSNPDASSQSLSSILNNPHAGKSDAS 60

Query: 61   WAAWWSSSSTVSPPEFLPLSSTIASSEVTRFDFNNYIALISDSYHRFEDIRNHSTKESVG 120
            W  WWSSSSTV+PPEF+PL+S+ ASSEVTRFDFNNY ALISDSY+RFEDIRNHS+KE+ G
Sbjct: 61   WVGWWSSSSTVTPPEFIPLTSSTASSEVTRFDFNNYTALISDSYNRFEDIRNHSSKENGG 120

Query: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQEKLSHYLDV 180
            LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNV+QNLVLQEKLSHYLDV
Sbjct: 121  LDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVSQNLVLQEKLSHYLDV 180

Query: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDLVDSAREIQE 240
            VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSR+RQLKETIRLLDVDLVDSAR+IQE
Sbjct: 181  VELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRLRQLKETIRLLDVDLVDSARQIQE 240

Query: 241  QNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLLEGDELSGLH 300
            QNATRNNLLALQQKLKLILYVNQAISALKLLVAS DCAGALDVTDDLLHLLEGDEL+GLH
Sbjct: 241  QNATRNNLLALQQKLKLILYVNQAISALKLLVASADCAGALDVTDDLLHLLEGDELAGLH 300

Query: 301  CFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLMNGKDEVKF- 360
            CFRHLRDHVAAS+ESITSILSAEFMRAS HDAGDVDLVII ETKA  SNLMNGKDEVK  
Sbjct: 301  CFRHLRDHVAASLESITSILSAEFMRASIHDAGDVDLVIISETKASISNLMNGKDEVKLT 360

Query: 361  ---DEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAELLPVLLM 420
               DEEE SNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVT DMKTAIKNAVAELLPVLL+
Sbjct: 361  CILDEEETSNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTVDMKTAIKNAVAELLPVLLV 420

Query: 421  RPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRAAEVKKSI 480
            RPLDSDFAPGERT D DGG ASLASKLRGLSSEGFVQLL+AIFKIVQVHLVRAAEVKKSI
Sbjct: 421  RPLDSDFAPGERTKDTDGG-ASLASKLRGLSSEGFVQLLNAIFKIVQVHLVRAAEVKKSI 480

Query: 481  EWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVTSLPGKAN 540
            EWIMCNLDGHYAADSVAAAIA GAAA+GTAQDSDNQ GL LPH+PQ  AAKVTSL GKAN
Sbjct: 481  EWIMCNLDGHYAADSVAAAIATGAAASGTAQDSDNQGGLPLPHVPQG-AAKVTSLQGKAN 540

Query: 541  DAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVHIILDVQK 600
            DAANPSNMSRNFR                                               
Sbjct: 541  DAANPSNMSRNFR----------------------------------------------- 600

Query: 601  LASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKLLGVRILV 660
                                        ADVLRENTEAVFAACDAAHGRWAKLLGVRILV
Sbjct: 601  ----------------------------ADVLRENTEAVFAACDAAHGRWAKLLGVRILV 660

Query: 661  HPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKIKAV 720
            HPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHE RMTKIKAV
Sbjct: 661  HPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHEFRMTKIKAV 720

Query: 721  LDQETWVEVDVPDEFQAIAESLYSQELLSAKIDDAQGNMDRSYTDVTTNNDDSSIVGGGS 780
            LD ETWVEVDVPDEFQ IAESL   ELLSAK+DD+QGNMD+SY+DV TNN+D+ I+GG  
Sbjct: 721  LDVETWVEVDVPDEFQTIAESLCFHELLSAKLDDSQGNMDQSYSDVATNNEDARIIGG-- 780

Query: 781  LNAQQHSELTDSSDMTGGNTEHAKPTPADKIEKSKADVLIPTTQINNTNVKERGKSSSQT 840
            +NAQQHSE  DSSD++G NTEH KPTPAD IE SKADV IP TQ NNTNVKERGKSSS T
Sbjct: 781  VNAQQHSEQVDSSDISGRNTEHVKPTPADTIENSKADVAIPATQNNNTNVKERGKSSSLT 840

Query: 841  LQYKGIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNTRTCQLVLGA 900
            LQYKG+GYHM         MLSEY+DMNDSLPALSSE+VHRVVEILKFFNTRTCQLVLGA
Sbjct: 841  LQYKGVGYHMVNCGLILLKMLSEYVDMNDSLPALSSEIVHRVVEILKFFNTRTCQLVLGA 900

Query: 901  GAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLISEIDRVAQDY 960
            GAMQVSGLKSITSKHLALASQVISFT AIIPE+RRILFLKVPEARKTLL+SEIDRVAQDY
Sbjct: 901  GAMQVSGLKSITSKHLALASQVISFTFAIIPEMRRILFLKVPEARKTLLLSEIDRVAQDY 960

Query: 961  KVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLTKEVGYLQRV 1020
            KVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRL+D+DPQPSQFARSLTKEVGYLQRV
Sbjct: 961  KVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLEDADPQPSQFARSLTKEVGYLQRV 1020

Query: 1021 LSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCD 1080
            LSRTLHEADV+AIF+QVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCD
Sbjct: 1021 LSRTLHEADVKAIFRQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHILGCIRSLPCD 1027

Query: 1081 DLSKPDTPNWGQLDEFLDQRFGSEAG 1090
            D SKPD PNWGQLDEFLDQR GSEAG
Sbjct: 1081 DSSKPDIPNWGQLDEFLDQRLGSEAG 1027

BLAST of MC01g1658 vs. TAIR 10
Match: AT4G19490.1 (VPS54 )

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 670/1113 (60.20%), Postives = 807/1113 (72.51%), Query Frame = 0

Query: 1    MESQPSQSGRSPT---EYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGK- 60
            M+S PS  GRS T     S  L R +S   ++ S   K+ SDASSQSLSSILNNPH GK 
Sbjct: 1    MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60

Query: 61   ----SDASWAAWWSSSSTVSPPEFLPLSST-IASSEVTRFDFNNYIALISDSYHRFEDIR 120
                SDASW  WWSSS+ V+P EF P++ST +  SE+TR DF+ Y++ IS+S+ RFEDIR
Sbjct: 61   GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120

Query: 121  NHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQ 180
             H+ +ES G D        L ACLREVP+LYFKEDFALEDGATFR+ACPF ++ +NL LQ
Sbjct: 121  KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180

Query: 181  EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDL 240
            EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR+LKETIRLLD +L
Sbjct: 181  EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240

Query: 241  VDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLL 300
            VDSAR+IQE ++TR N+L LQ+KL+LILYVNQA+SALKLLVAS DCAGALD+TDDL +LL
Sbjct: 241  VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300

Query: 301  EGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLM 360
             GDEL+GL+CFRHLRDHV +SI+SI SIL++EFMR S HD G++D++I+     R S   
Sbjct: 301  AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360

Query: 361  NGK--DEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAE 420
            NG   +EVK +EE+ S   DRLLP++IGLLRTAK PS+LR+YRD +T++MK AIK AVA+
Sbjct: 361  NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420

Query: 421  LLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRA 480
            LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AIFKIVQ HLVRA
Sbjct: 421  LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480

Query: 481  AEVKKSIEWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVT 540
            +EVKK+IEWI+CN+DGHYAADSVAAAIA+GA AA TAQ+   Q G L+     +  +K  
Sbjct: 481  SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540

Query: 541  SLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVH 600
             L GK++DA++  NMSRNFR                                        
Sbjct: 541  PLQGKSSDASSLMNMSRNFR---------------------------------------- 600

Query: 601  IILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKL 660
                                               ADVLRENTEAVFAAC+  HGRWAKL
Sbjct: 601  -----------------------------------ADVLRENTEAVFAACEVTHGRWAKL 660

Query: 661  LGVRILVHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESR 720
            LGVR L+HPKL+LQEF+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESR
Sbjct: 661  LGVRALLHPKLKLQEFMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESR 720

Query: 721  MTKIKAVLDQETWVEVDVPDEFQAIAESLY-SQELLSAKIDDAQGNMDRSYTDVTTNNDD 780
            MTK+KAVLDQETW E+DVP+EFQ+I  SL+ SQ L+S K+DDA  N   SY       + 
Sbjct: 721  MTKLKAVLDQETWDEIDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNG 780

Query: 781  SSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPA-DKIEKSKADVLIPTTQINNTNVK 840
            S   G G  N++  +E ++SS+ +  +    KPT + + +E+SKA V   T   N +N K
Sbjct: 781  SLTSGSGDQNSELRNEKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQK 840

Query: 841  ERGKSSSQTLQYKGIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNT 900
              GKS+   L Y+G+GYHM         MLSEYIDMN+SLPALSSE+V RVVE+L+FFNT
Sbjct: 841  AHGKSN---LFYQGVGYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNT 900

Query: 901  RTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLIS 960
            RTCQLVLGAGAMQVSGLKSI +KHLALASQVI FT  IIPE RRILF KVPE RK LL  
Sbjct: 901  RTCQLVLGAGAMQVSGLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSV 960

Query: 961  EIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLT 1020
            EID+VAQD+++HRDEI+TKLVQIMRERLL HL GLP++VE WNR  D++ Q  +FA  LT
Sbjct: 961  EIDKVAQDFRIHRDEIYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLT 1020

Query: 1021 KEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHIL 1080
            +EVGYL RVLS TLHEADVQAIF+QV+ I H Q S+  + L+IS+ +AK RL   V+ IL
Sbjct: 1021 REVGYLHRVLSETLHEADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELIL 1028

Query: 1081 GCIRSLPCDDLSKPDTPNWGQLDEFLDQRFGSE 1089
             CIRSLP D+ ++ D PNWGQLDEF  + F  E
Sbjct: 1081 KCIRSLPSDNANQSDIPNWGQLDEFFAEHFREE 1028

BLAST of MC01g1658 vs. TAIR 10
Match: AT4G19490.2 (VPS54 )

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 670/1113 (60.20%), Postives = 807/1113 (72.51%), Query Frame = 0

Query: 1    MESQPSQSGRSPT---EYSTLLSRETSLRRTTSS---KTNSDASSQSLSSILNNPHAGK- 60
            M+S PS  GRS T     S  L R +S   ++ S   K+ SDASSQSLSSILNNPH GK 
Sbjct: 1    MDSHPSLMGRSITNSNRSSLDLGRPSSSSSSSPSPLTKSISDASSQSLSSILNNPHGGKS 60

Query: 61   ----SDASWAAWWSSSSTVSPPEFLPLSST-IASSEVTRFDFNNYIALISDSYHRFEDIR 120
                SDASW  WWSSS+ V+P EF P++ST +  SE+TR DF+ Y++ IS+S+ RFEDIR
Sbjct: 61   GVYGSDASWVGWWSSSTFVAPAEFAPVASTKLPGSELTRSDFHGYVSSISESHGRFEDIR 120

Query: 121  NHSTKESVGLDSIGGQGEALVACLREVPALYFKEDFALEDGATFRAACPFLNVTQNLVLQ 180
             H+ +ES G D        L ACLREVP+LYFKEDFALEDGATFR+ACPF ++ +NL LQ
Sbjct: 121  KHTREESCGFDQ-ESHVSGLAACLREVPSLYFKEDFALEDGATFRSACPFSSLNENLALQ 180

Query: 181  EKLSHYLDVVELHLVKEISLRSNSFFEAQGQLQDLNVKIVEGCSRIRQLKETIRLLDVDL 240
            EKLS YLDVVELHLVKEIS+RS+SFFEAQGQLQDLNVKIVEGCSRIR+LKETIRLLD +L
Sbjct: 181  EKLSQYLDVVELHLVKEISVRSDSFFEAQGQLQDLNVKIVEGCSRIRELKETIRLLDRNL 240

Query: 241  VDSAREIQEQNATRNNLLALQQKLKLILYVNQAISALKLLVASGDCAGALDVTDDLLHLL 300
            VDSAR+IQE ++TR N+L LQ+KL+LILYVNQA+SALKLLVAS DCAGALD+TDDL +LL
Sbjct: 241  VDSARQIQELSSTRINMLELQRKLRLILYVNQALSALKLLVASADCAGALDITDDLQNLL 300

Query: 301  EGDELSGLHCFRHLRDHVAASIESITSILSAEFMRASFHDAGDVDLVIIFETKARASNLM 360
             GDEL+GL+CFRHLRDHV +SI+SI SIL++EFMR S HD G++D++I+     R S   
Sbjct: 301  AGDELTGLYCFRHLRDHVTSSIDSINSILTSEFMRISIHDTGEIDVLILSAANIRGSISS 360

Query: 361  NGK--DEVKFDEEEASNFRDRLLPIIIGLLRTAKLPSVLRLYRDAVTADMKTAIKNAVAE 420
            NG   +EVK +EE+ S   DRLLP++IGLLRTAK PS+LR+YRD +T++MK AIK AVA+
Sbjct: 361  NGNTGEEVKLEEEDTSTLCDRLLPLVIGLLRTAKFPSILRMYRDTLTSEMKNAIKKAVAD 420

Query: 421  LLPVLLMRPLDSDFAPGERTVDADGGGASLASKLRGLSSEGFVQLLSAIFKIVQVHLVRA 480
            LLP+L+ R L+SDF+ GER+VD DGGG SLASKLR LSSE FV LL+AIFKIVQ HLVRA
Sbjct: 421  LLPILVARSLESDFSHGERSVD-DGGGLSLASKLRTLSSEAFVNLLTAIFKIVQAHLVRA 480

Query: 481  AEVKKSIEWIMCNLDGHYAADSVAAAIAIGAAAAGTAQDSDNQVGLLLPHLPQRVAAKVT 540
            +EVKK+IEWI+CN+DGHYAADSVAAAIA+GA AA TAQ+   Q G L+     +  +K  
Sbjct: 481  SEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIGFQGGSLVSSPLGKATSKAP 540

Query: 541  SLPGKANDAANPSNMSRNFRYCFVVLIYFTFPFSFGGRGELCANLVRVKLSSKILYHSVH 600
             L GK++DA++  NMSRNFR                                        
Sbjct: 541  PLQGKSSDASSLMNMSRNFR---------------------------------------- 600

Query: 601  IILDVQKLASTLALSFPLSAIILVEKEEMFERWKKADVLRENTEAVFAACDAAHGRWAKL 660
                                               ADVLRENTEAVFAAC+  HGRWAKL
Sbjct: 601  -----------------------------------ADVLRENTEAVFAACEVTHGRWAKL 660

Query: 661  LGVRILVHPKLRLQEFLSIYNITQDFITATEKIGGRLGYSIRGTLQSQAKAFVDFQHESR 720
            LGVR L+HPKL+LQEF+SIY++TQ+FIT+TEKIGGRLG SIRGTLQSQAKAFVD QHESR
Sbjct: 661  LGVRALLHPKLKLQEFMSIYDLTQEFITSTEKIGGRLGSSIRGTLQSQAKAFVDSQHESR 720

Query: 721  MTKIKAVLDQETWVEVDVPDEFQAIAESLY-SQELLSAKIDDAQGNMDRSYTDVTTNNDD 780
            MTK+KAVLDQETW E+DVP+EFQ+I  SL+ SQ L+S K+DDA  N   SY       + 
Sbjct: 721  MTKLKAVLDQETWDEIDVPEEFQSIISSLFASQRLISGKVDDADLN---SYHSNRLPLNG 780

Query: 781  SSIVGGGSLNAQQHSELTDSSDMTGGNTEHAKPTPA-DKIEKSKADVLIPTTQINNTNVK 840
            S   G G  N++  +E ++SS+ +  +    KPT + + +E+SKA V   T   N +N K
Sbjct: 781  SLTSGSGDQNSELRNEKSESSEGSVVSDAQVKPTVSPESLERSKAGVSSATN--NQSNQK 840

Query: 841  ERGKSSSQTLQYKGIGYHM---------MLSEYIDMNDSLPALSSEVVHRVVEILKFFNT 900
              GKS+   L Y+G+GYHM         MLSEYIDMN+SLPALSSE+V RVVE+L+FFNT
Sbjct: 841  AHGKSN---LFYQGVGYHMVNCGLILLKMLSEYIDMNNSLPALSSEIVLRVVEVLRFFNT 900

Query: 901  RTCQLVLGAGAMQVSGLKSITSKHLALASQVISFTCAIIPEIRRILFLKVPEARKTLLIS 960
            RTCQLVLGAGAMQVSGLKSI +KHLALASQVI FT  IIPE RRILF KVPE RK LL  
Sbjct: 901  RTCQLVLGAGAMQVSGLKSIKAKHLALASQVIDFTYTIIPETRRILFSKVPETRKPLLSV 960

Query: 961  EIDRVAQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIVESWNRLQDSDPQPSQFARSLT 1020
            EID+VAQD+++HRDEI+TKLVQIMRERLL HL GLP++VE WNR  D++ Q  +FA  LT
Sbjct: 961  EIDKVAQDFRIHRDEIYTKLVQIMRERLLAHLHGLPKVVEGWNRPPDTNKQTKEFAWPLT 1020

Query: 1021 KEVGYLQRVLSRTLHEADVQAIFKQVVKIFHLQISEAFSRLDISTPQAKDRLLRDVKHIL 1080
            +EVGYL RVLS TLHEADVQAIF+QV+ I H Q S+  + L+IS+ +AK RL   V+ IL
Sbjct: 1021 REVGYLHRVLSETLHEADVQAIFRQVISIIHTQTSQTLTNLEISSTEAKKRLKLHVELIL 1028

Query: 1081 GCIRSLPCDDLSKPDTPNWGQLDEFLDQRFGSE 1089
             CIRSLP D+ ++ D PNWGQLDEF  + F  E
Sbjct: 1081 KCIRSLPSDNANQSDIPNWGQLDEFFAEHFREE 1028

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JT760.0e+0060.20Vacuolar protein sorting-associated protein 54, chloroplastic OS=Arabidopsis tha... [more]
Q9JMK83.0e-5825.73Vacuolar protein sorting-associated protein 54 OS=Rattus norvegicus OX=10116 GN=... [more]
Q9P1Q05.1e-5825.34Vacuolar protein sorting-associated protein 54 OS=Homo sapiens OX=9606 GN=VPS54 ... [more]
Q5SPW01.4e-5525.19Vacuolar protein sorting-associated protein 54 OS=Mus musculus OX=10090 GN=Vps54... [more]
Q9VLC09.0e-3921.88Vacuolar protein sorting-associated protein 54 OS=Drosophila melanogaster OX=722... [more]
Match NameE-valueIdentityDescription
XP_022146560.10.092.27vacuolar protein sorting-associated protein 54, chloroplastic [Momordica charant... [more]
XP_038882115.10.085.84vacuolar protein sorting-associated protein 54, chloroplastic isoform X1 [Beninc... [more]
XP_004134530.10.085.17vacuolar protein sorting-associated protein 54, chloroplastic [Cucumis sativus] ... [more]
KAA0052432.10.084.71vacuolar protein sorting-associated protein 54 [Cucumis melo var. makuwa][more]
XP_008439467.10.084.62PREDICTED: vacuolar protein sorting-associated protein 54, chloroplastic [Cucumi... [more]
Match NameE-valueIdentityDescription
A0A6J1CXK30.092.27vacuolar protein sorting-associated protein 54, chloroplastic OS=Momordica chara... [more]
A0A0A0KMC90.085.17Vps54 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G526560 PE=3 ... [more]
A0A5A7U9K30.084.71Vacuolar protein sorting-associated protein 54 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3AYS90.084.62vacuolar protein sorting-associated protein 54, chloroplastic OS=Cucumis melo OX... [more]
A0A6J1EEU20.083.91vacuolar protein sorting-associated protein 54, chloroplastic-like OS=Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT4G19490.10.0e+0060.20VPS54 [more]
AT4G19490.20.0e+0060.20VPS54 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D6.10.250.860coord: 930..969
e-value: 5.5E-8
score: 34.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 779..793
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 779..803
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..46
NoneNo IPR availablePANTHERPTHR12965:SF1VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54, CHLOROPLASTICcoord: 1..547
NoneNo IPR availablePANTHERPTHR12965:SF1VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54, CHLOROPLASTICcoord: 618..1088
IPR012501Vacuolar protein sorting-associated protein 54, C-terminalPFAMPF07928Vps54coord: 844..962
e-value: 3.0E-41
score: 140.9
IPR039745Vacuolar protein sorting-associated protein 54PANTHERPTHR12965VACUOLAR PROTEIN SORTING 54coord: 618..1088
IPR039745Vacuolar protein sorting-associated protein 54PANTHERPTHR12965VACUOLAR PROTEIN SORTING 54coord: 1..547

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g1658.1MC01g1658.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006896 Golgi to vacuole transport
biological_process GO:0015031 protein transport
biological_process GO:0042147 retrograde transport, endosome to Golgi
cellular_component GO:0005829 cytosol
cellular_component GO:0000938 GARP complex
molecular_function GO:0019905 syntaxin binding