MC01g1503 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g1503
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.5-like
LocationMC01: 19378466 .. 19380705 (+)
RNA-Seq ExpressionMC01g1503
SyntenyMC01g1503
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGAGAAGTTTGAGGTAGTTGAAGGAAAGGTGGACTGGAAGGCAAGATCAGCTTTCAAACACAAACATGGAGGAACCAGATCATCTTTTCTCATACTAGGTAGAAAAATCATTTGCTCATCAAGTTTTTCAAATGTAGTGCCAAACATATTCTAACATAACATGGGGGCCTATGAAGAAAAAGAAAGATGACAGAGCGAGCTTCATAGCTGGCTACACTCACACCCCTGCATTCCATTGTAATCACAACACAGTTCATGACAGTCTTGTTTTACCTTTTGGGTTTCTGTTGCAGTTGCATTTGGTTTCGAGAATCTGGCGAATTTTGCGCTTGCAGTGAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCGGCCAACCAGCTGACAAATTACTTGGGTACCGGTTACATTATCTCTATTCTGATGGCTATTCTCGCAGACACATACTTGGGCAGAGTCAAAACCGTCATCATTTCCGGCTGCCTCGAGTTTCTGGTCTGCCCAACTTCACTGGCTTCAGTTTGCCGCCCCCGCTACCTATTTCCCATTCCACCATATTCTAACTGCAACTGTTATTTCAGGCGCTAATATTGCTCATGATACAAGCTCACTACCCAAAGCTCAAGCCACCGCCTTGTAATATGTTTGATAAACAGTCCCACTGCGAGACAGTTGAAGGCGGAAACGCTGCCCTCCTCTATGTTGCTCTCTACATATTGGCCATTGGTACTGCAGGAATTAAGGCCGCATTACCATCACATCGTGCTGATCAATTTGATGAAAAGGACCCCAAGGAAGCAATGCAAATGTCCAGCTTCTTCAACCACCTCTTGCTCGGAGTGTGCCTCGGTGGTGCTGTTAGTTTAACTCTAATTGTGTGGATCCAAGACTATAAAGGTTGGGACTGGGGTTTAGGGGTATCTTCTGCAGCCATCTTCTTCAGCGTGGTCATATTTGTCGCTGGAATACCCCTGTATCGGATGCATATTATTTCTGGATCTAGTGCAATAGTCCAAATTCTACAGGTATGTATGTACGTATAACAGAGTAAAAGAAGAGTTTGAGGTTCCATTCTAAAGAATTTCATCCAAATACATTCTCACTTCTGTCCATACGAATGTTCTTTTCAGGTCTATGGTGCAGCCATTCGTAATAGAAACCTTGTTCTCCCTGAAGACTCTGCAGATCTCTATGAGACTGTCAGGGACAAGGAGGCTGCTACGGAAGAAGACTTTCTGCCTCATAGAAACATTTACAGGTTTTGTGTATTTGAACCTTCTGTTAATTTGGCAGTCCAACAATAGAATTAAACGTTCGGATAACCCACCAATATGTACAGGTTTCTAGACAAAGCGGCAATTCAACAAACACCTTCAGGGCAAGTTGAAACGCCCGAAGCTTCAAATCCTTGGAAATTATGCAGAGTCACCCAAGTAGAGAATGCGAAAATAATTCTGAGTATGGTACCAATATTCTGCTGCACAATTGTAATGACCCTCTGTTTGGCTCAGCTCCAAACCTTCTCCATCCAACAAGGGCTCACCATGGACACAAAACTCACCAATTCTTTCCACATCCCCCCCGCCTCGCTCCCCATCATCCCAGTCTCCTTCATCATCCTCATAGTTCCAATCTACGACAGGATTTTCGTACCTTTTGCGCGAAAGCTCACGGGCATCCCCACCGGAATCACCCACTTGCAACGAGTAGGGGTGGGATTGGTTCTGTCGAGCATGTCCATGGCCGTCGCGGCGCTAATGGAAGTGAAGCGCAAGGGCGTGGCGAGAGACCACAACATGCTGGACGCAACCCCTGTATCGCAGCCATTGCCAATCAGCACGTTCTGGCTATCCTTTCAGTTCTTCATATTCGGAATCGCAGATCTGTTCACGTACGTGGGACTTCTGGAATTCTTCTACTCCGAGGCGCCGAAAGCCCTGAAATCCGTGTCCACTTGCTTCCTGTGGAGTTCGATGGCCTTGGGCTACTTCTTGAGCACCATAGTTGTGAAGGTTGTGAACAGTGTTACGAAGGGGATTACAAGCAGTGGAGGCTGGTTGATTGGAAACAACATTAATCGGAACCATCTGAACCTCTTCTACTGGCTGCTTTCAATCTTGAGCTTGATCAACTTCTCGATCTATTTATTTGTTGCAAAGAGATACAAATACAGAAACCATAATAAGCCCGCCATCTCCGATGCTGACAATAGGAAGCCA

mRNA sequence

ATGCAGGAGAAGTTTGAGGTAGTTGAAGGAAAGGTGGACTGGAAGGCAAGATCAGCTTTCAAACACAAACATGGAGGAACCAGATCATCTTTTCTCATACTAGTTGCATTTGGTTTCGAGAATCTGGCGAATTTTGCGCTTGCAGTGAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCGGCCAACCAGCTGACAAATTACTTGGGTACCGGTTACATTATCTCTATTCTGATGGCTATTCTCGCAGACACATACTTGGGCAGAGTCAAAACCGTCATCATTTCCGGCTGCCTCGAGTTTCTGGCGCTAATATTGCTCATGATACAAGCTCACTACCCAAAGCTCAAGCCACCGCCTTGTAATATGTTTGATAAACAGTCCCACTGCGAGACAGTTGAAGGCGGAAACGCTGCCCTCCTCTATGTTGCTCTCTACATATTGGCCATTGGTACTGCAGGAATTAAGGCCGCATTACCATCACATCGTGCTGATCAATTTGATGAAAAGGACCCCAAGGAAGCAATGCAAATGTCCAGCTTCTTCAACCACCTCTTGCTCGGAGTGTGCCTCGGTGGTGCTGTTAGTTTAACTCTAATTGTGTGGATCCAAGACTATAAAGGTTGGGACTGGGGTTTAGGGGTATCTTCTGCAGCCATCTTCTTCAGCGTGGTCATATTTGTCGCTGGAATACCCCTGTATCGGATGCATATTATTTCTGGATCTAGTGCAATAGTCCAAATTCTACAGGTCTATGGTGCAGCCATTCGTAATAGAAACCTTGTTCTCCCTGAAGACTCTGCAGATCTCTATGAGACTGTCAGGGACAAGGAGGCTGCTACGGAAGAAGACTTTCTGCCTCATAGAAACATTTACAGGTTTCTAGACAAAGCGGCAATTCAACAAACACCTTCAGGGCAAGTTGAAACGCCCGAAGCTTCAAATCCTTGGAAATTATGCAGAGTCACCCAAGTAGAGAATGCGAAAATAATTCTGAGTATGGTACCAATATTCTGCTGCACAATTGTAATGACCCTCTGTTTGGCTCAGCTCCAAACCTTCTCCATCCAACAAGGGCTCACCATGGACACAAAACTCACCAATTCTTTCCACATCCCCCCCGCCTCGCTCCCCATCATCCCAGTCTCCTTCATCATCCTCATAGTTCCAATCTACGACAGGATTTTCGTACCTTTTGCGCGAAAGCTCACGGGCATCCCCACCGGAATCACCCACTTGCAACGAGTAGGGGTGGGATTGGTTCTGTCGAGCATGTCCATGGCCGTCGCGGCGCTAATGGAAGTGAAGCGCAAGGGCGTGGCGAGAGACCACAACATGCTGGACGCAACCCCTGTATCGCAGCCATTGCCAATCAGCACGTTCTGGCTATCCTTTCAGTTCTTCATATTCGGAATCGCAGATCTGTTCACGTACGTGGGACTTCTGGAATTCTTCTACTCCGAGGCGCCGAAAGCCCTGAAATCCGTGTCCACTTGCTTCCTGTGGAGTTCGATGGCCTTGGGCTACTTCTTGAGCACCATAGTTGTGAAGGTTGTGAACAGTGTTACGAAGGGGATTACAAGCAGTGGAGGCTGGTTGATTGGAAACAACATTAATCGGAACCATCTGAACCTCTTCTACTGGCTGCTTTCAATCTTGAGCTTGATCAACTTCTCGATCTATTTATTTGTTGCAAAGAGATACAAATACAGAAACCATAATAAGCCCGCCATCTCCGATGCTGACAATAGGAAGCCA

Coding sequence (CDS)

ATGCAGGAGAAGTTTGAGGTAGTTGAAGGAAAGGTGGACTGGAAGGCAAGATCAGCTTTCAAACACAAACATGGAGGAACCAGATCATCTTTTCTCATACTAGTTGCATTTGGTTTCGAGAATCTGGCGAATTTTGCGCTTGCAGTGAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCGGCCAACCAGCTGACAAATTACTTGGGTACCGGTTACATTATCTCTATTCTGATGGCTATTCTCGCAGACACATACTTGGGCAGAGTCAAAACCGTCATCATTTCCGGCTGCCTCGAGTTTCTGGCGCTAATATTGCTCATGATACAAGCTCACTACCCAAAGCTCAAGCCACCGCCTTGTAATATGTTTGATAAACAGTCCCACTGCGAGACAGTTGAAGGCGGAAACGCTGCCCTCCTCTATGTTGCTCTCTACATATTGGCCATTGGTACTGCAGGAATTAAGGCCGCATTACCATCACATCGTGCTGATCAATTTGATGAAAAGGACCCCAAGGAAGCAATGCAAATGTCCAGCTTCTTCAACCACCTCTTGCTCGGAGTGTGCCTCGGTGGTGCTGTTAGTTTAACTCTAATTGTGTGGATCCAAGACTATAAAGGTTGGGACTGGGGTTTAGGGGTATCTTCTGCAGCCATCTTCTTCAGCGTGGTCATATTTGTCGCTGGAATACCCCTGTATCGGATGCATATTATTTCTGGATCTAGTGCAATAGTCCAAATTCTACAGGTCTATGGTGCAGCCATTCGTAATAGAAACCTTGTTCTCCCTGAAGACTCTGCAGATCTCTATGAGACTGTCAGGGACAAGGAGGCTGCTACGGAAGAAGACTTTCTGCCTCATAGAAACATTTACAGGTTTCTAGACAAAGCGGCAATTCAACAAACACCTTCAGGGCAAGTTGAAACGCCCGAAGCTTCAAATCCTTGGAAATTATGCAGAGTCACCCAAGTAGAGAATGCGAAAATAATTCTGAGTATGGTACCAATATTCTGCTGCACAATTGTAATGACCCTCTGTTTGGCTCAGCTCCAAACCTTCTCCATCCAACAAGGGCTCACCATGGACACAAAACTCACCAATTCTTTCCACATCCCCCCCGCCTCGCTCCCCATCATCCCAGTCTCCTTCATCATCCTCATAGTTCCAATCTACGACAGGATTTTCGTACCTTTTGCGCGAAAGCTCACGGGCATCCCCACCGGAATCACCCACTTGCAACGAGTAGGGGTGGGATTGGTTCTGTCGAGCATGTCCATGGCCGTCGCGGCGCTAATGGAAGTGAAGCGCAAGGGCGTGGCGAGAGACCACAACATGCTGGACGCAACCCCTGTATCGCAGCCATTGCCAATCAGCACGTTCTGGCTATCCTTTCAGTTCTTCATATTCGGAATCGCAGATCTGTTCACGTACGTGGGACTTCTGGAATTCTTCTACTCCGAGGCGCCGAAAGCCCTGAAATCCGTGTCCACTTGCTTCCTGTGGAGTTCGATGGCCTTGGGCTACTTCTTGAGCACCATAGTTGTGAAGGTTGTGAACAGTGTTACGAAGGGGATTACAAGCAGTGGAGGCTGGTTGATTGGAAACAACATTAATCGGAACCATCTGAACCTCTTCTACTGGCTGCTTTCAATCTTGAGCTTGATCAACTTCTCGATCTATTTATTTGTTGCAAAGAGATACAAATACAGAAACCATAATAAGCCCGCCATCTCCGATGCTGACAATAGGAAGCCA

Protein sequence

MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIGNNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADNRKP
Homology
BLAST of MC01g1503 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 6.0e-130
Identity = 258/598 (43.14%), Postives = 379/598 (63.38%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           ++E+    EG  DW+ R+A K +HGG  ++  +LV    ENLA  A A NL+ Y    MH
Sbjct: 3   VEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMH 62

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
              + +AN +TN++GT +++++L   L+D +    +  +IS  +EFL LI+L IQA  P 
Sbjct: 63  MSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPS 122

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           L PP C+       CE V G  AA+L+V LY++A+G  GIK +L SH A+QFDE  PK  
Sbjct: 123 LMPPSCD----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGR 182

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
            Q S+FFN+ +  +  G  V++T +VW++D KGW+WG GVS+ AIF S++IF++G   YR
Sbjct: 183 KQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYR 242

Query: 241 MHIISGS--SAIVQIL---------------QVYGAAIRNRNLVLPEDSADLYETVRDKE 300
             I  GS  + I+++L                V   ++   N  + +   ++ E+  + E
Sbjct: 243 NKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEV-ESQGELE 302

Query: 301 AATEEDFLPHR----NIYRFLDKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSM 360
              +E+ LP R    N  + L+ AA ++     +E          C V QVE+ KI+L M
Sbjct: 303 KPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLE----------CTVQQVEDVKIVLKM 362

Query: 361 VPIFCCTIVMTLCLAQLQTFSIQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDR 420
           +PIF CTI++  CLAQL TFS+QQ  +M+TK+  S  IPPASLPI PV FI+++ PIYD 
Sbjct: 363 LPIFACTIMLNCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDH 422

Query: 421 IFVPFARKLTGIPTGITHLQRVGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQ 480
           + +PFARK T   TG+THLQR+GVGLVLS ++MAVAAL+E+KRKGVA+D  +LD+    +
Sbjct: 423 LIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDS---KE 482

Query: 481 PLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI 540
            LP++  W++ Q+   G ADLFT  GLLE+F++EAP +++S++T   W+S+A+GY+LS++
Sbjct: 483 TLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSV 542

Query: 541 VVKVVNSVTKGITSSGGWLIGNNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRN 578
           +V +VNS+T G + +  WL G +INR  L+ FYWL+ +LS  NF  YLF A RYKYR+
Sbjct: 543 IVSIVNSIT-GSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 580

BLAST of MC01g1503 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.2e-125
Identity = 248/567 (43.74%), Postives = 357/567 (62.96%), Query Frame = 0

Query: 9   EGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAAN 68
           EG  DW+ ++A + +HGG  ++  +L     ENLA  A A NL+ Y  + MH  LA +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 69  QLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNM 128
           ++T ++ T +++++L   LAD +       +IS  +EFL LILL IQA  P L PPPC  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS 132

Query: 129 FDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFN 188
                 CE V G  AA L+V LY++++G  GIK +LPSH A+QFDE  PK   Q S+FFN
Sbjct: 133 -SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 189 HLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSS 248
           + +  +  G  V++T +VWI+D KGW+WG GVS+ +IF S+++F+ G   Y+  I  GS 
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 252

Query: 249 AIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDKAAIQQT 308
                  +  A+I + +     +     E   + E  T    L   N    L+KA   +T
Sbjct: 253 LTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL--TNSLTCLNKAIEGKT 312

Query: 309 PSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMD 368
                      + W  C V QVE+ KI+L M+PIF CTI++  CLAQL T+S+ Q  TM+
Sbjct: 313 ----------HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMN 372

Query: 369 TKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVGVGLVLS 428
            K+ N F++P ASLP+ PV F++++ P YD + +PFARK+T    GITHLQR+GVGLVLS
Sbjct: 373 RKIVN-FNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLS 432

Query: 429 SMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLE 488
            ++MAVAAL+E+KRK VAR+  +LD+    + LPI+  W++ Q+   G ADLFT  GLLE
Sbjct: 433 IVAMAVAALVELKRKQVAREAGLLDS---EETLPITFLWIALQYLFLGSADLFTLAGLLE 492

Query: 489 FFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIGNNINRNHL 548
           FF++EAP +++S++T   W+S+ALGY+LS+++V +VN VTK    S  WL G  +NRN L
Sbjct: 493 FFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQS-PWL-GEKLNRNRL 552

Query: 549 NLFYWLLSILSLINFSIYLFVAKRYKY 576
           +LFYWL+ +LS++NF  YLF AKRYKY
Sbjct: 553 DLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of MC01g1503 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 7.3e-120
Identity = 243/560 (43.39%), Postives = 348/560 (62.14%), Query Frame = 0

Query: 9   EGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAAN 68
           E  VDW+ R +  +KHGG R++  +L    FE +   A+  NLITY  + MHF L+ AAN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 69  QLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNM 128
            +TN++GT +I ++L   L+D +LG   T+II G +E    ILL +QAH P+LKPP CN 
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 129 FDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFN 188
              Q+ CE  +G  A + ++ALY++A+G+  +K  + +H ADQF +  PK++ ++SS+FN
Sbjct: 147 LIDQT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFN 206

Query: 189 HLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSS 248
                  +G  ++LTL+VW+Q + G D G GVS+AA+   ++  V+G  +Y  +     S
Sbjct: 207 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSG-TMYFRNKRPRRS 266

Query: 249 AIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDKAAIQQT 308
               I  V  AAI  R L  P D   L+              LPH   +RFLDKA I+  
Sbjct: 267 IFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQ 326

Query: 309 PSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMD 368
                +T    +PW+LC VTQVE  K ++S+VPIF  TIV    LAQLQTFS+QQG +M+
Sbjct: 327 -----DTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 386

Query: 369 TKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVGVGLVLS 428
           T+L+NSFHIPPASL  IP   +I +VP+YD   VPFARKLTG  +GI  L R+G+GL LS
Sbjct: 387 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 446

Query: 429 SMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLE 488
           + SM  AA++E KR    RD ++LD         +S FW++ QF IFGI+++FT VGL+E
Sbjct: 447 TFSMVSAAMLEKKR----RDSSVLDGR------ILSIFWITPQFLIFGISEMFTAVGLIE 506

Query: 489 FFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIGNNINRNHL 548
           FFY ++ K ++S      + S + G++ S+++V VVN +T     S GWL  N++N++ L
Sbjct: 507 FFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRL 566

Query: 549 NLFYWLLSILSLINFSIYLF 569
           +LFYWLL++LSL+NF  YLF
Sbjct: 567 DLFYWLLAVLSLLNFLSYLF 569

BLAST of MC01g1503 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 5.4e-115
Identity = 236/564 (41.84%), Postives = 347/564 (61.52%), Query Frame = 0

Query: 12  VDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAANQLT 71
           VD++ R     KHGGTR++  +L    FE +A  A+  NLITY  + MHF L+ +AN +T
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 72  NYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNMFDK 131
           N++GT +++S+L   L+D+YLG  +T+++ G +E    ILL +QAH P+L+PP CNM   
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 132 QSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFNHLL 191
             HC    G  AA LY AL ++A+G+  +K  + SH A+QF  KD +   ++SSFFN   
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKDLR---KLSSFFNAAY 206

Query: 192 LGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSSAIV 251
               +G  ++LTL+VW+Q + G D G GVS+A +   ++  VAG   YR    SG S   
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSG-SIFT 266

Query: 252 QILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDKAAIQQTPSG 311
            I QV+ AAI  R  + P +   +++   D         L H N +RFLDKA I      
Sbjct: 267 PIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKP---LLHSNKFRFLDKACI------ 326

Query: 312 QVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMDTKL 371
           + +     +PW+LC + QV   KI+LS++PIF CTI+    LAQLQTFS+QQG +M+T +
Sbjct: 327 KTQGKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 386

Query: 372 TNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVGVGLVLSSMS 431
           T +F IPPASL  IP   +I  VP+Y+  FVP ARKLTG  +GI+ LQR+G GL L++ S
Sbjct: 387 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 446

Query: 432 MAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFY 491
           M  AAL+E KR+    + N++          +S FW++ QF IFG++++FT VGL+EFFY
Sbjct: 447 MVAAALVEKKRRESFLEQNVM----------LSIFWIAPQFLIFGLSEMFTAVGLVEFFY 506

Query: 492 SEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVT--KGITSSGGWLIGNNINRNHLN 551
            ++ ++++S  T   + S + G++LS+++V  VN VT   G  +  GWL  N++N++ L+
Sbjct: 507 KQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLD 566

Query: 552 LFYWLLSILSLINFSIYLFVAKRY 574
            FYWLL+ LS INF  YLF ++ Y
Sbjct: 567 HFYWLLASLSFINFFNYLFWSRWY 567

BLAST of MC01g1503 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 397.5 bits (1020), Expect = 2.6e-109
Identity = 232/580 (40.00%), Postives = 347/580 (59.83%), Query Frame = 0

Query: 10  GKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAANQ 69
           G VDW++R A + +HGG  ++  +LV    ENLA  A A NL+ Y ++ M F  + AAN 
Sbjct: 43  GYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPSGAANA 102

Query: 70  LTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNMF 129
           +T ++GT + +++L   LAD +       ++S  +EFL L++L +QAH    +P      
Sbjct: 103 VTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEP------ 162

Query: 130 DKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFNH 189
                       +   L+V LY++A+G  GIK +LP H A+QFDE+      Q S FFN+
Sbjct: 163 -----------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNY 222

Query: 190 LLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSSA 249
            +  +  G  +++T++VW++D KGW +G GVS+AAI  SV +F+AG  +YR+ + SG S 
Sbjct: 223 FIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSG-SP 282

Query: 250 IVQILQVYGAAI--------RNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLD 309
           I  + +V  AA+         +R +V      D  ++V  +    ++ FL       FL 
Sbjct: 283 ITTLFKVLTAALYAKYKKRRTSRIVVTCHTRNDCDDSVTKQNCDGDDGFLG-----SFL- 342

Query: 310 KAAIQQTPSGQVETPEASNPWKL-CRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 369
                    G+V     S P  L C   QV++ KI++ ++PIF  TI++  CLAQL TFS
Sbjct: 343 ---------GEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFS 402

Query: 370 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 429
           +QQ  TM+TKL  SF +PPA+LP+ PV F++++ P Y+ + +P ARK T   TGITHLQR
Sbjct: 403 VQQASTMNTKL-GSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQR 462

Query: 430 VGVGLVLSSMSMAVAALMEVKRKGVARD--HNMLDATPVSQPLPISTFWLSFQFFIFGIA 489
           +G GLVLS ++MAVAAL+E KRK V      N   ++  S PLPI+  W++ Q+   G A
Sbjct: 463 IGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSA 522

Query: 490 DLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWL 549
           DLFT  G++EFF++EAP  ++S++T   W+S+A+GY+ S+++V  VN VT G+     WL
Sbjct: 523 DLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVT-GLNHHNPWL 582

Query: 550 IGNNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNH 579
           +G N+N+ HL  FYWL+ +LS INF  YLF A RY YR++
Sbjct: 583 LGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSN 587

BLAST of MC01g1503 vs. NCBI nr
Match: XP_022142057.1 (protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia])

HSP 1 Score: 1149 bits (2973), Expect = 0.0
Identity = 591/591 (100.00%), Postives = 591/591 (100.00%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH
Sbjct: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA
Sbjct: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR
Sbjct: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL
Sbjct: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADNRKP 591
           NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADNRKP
Sbjct: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADNRKP 591

BLAST of MC01g1503 vs. NCBI nr
Match: XP_023003153.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima])

HSP 1 Score: 1037 bits (2681), Expect = 0.0
Identity = 527/578 (91.18%), Postives = 548/578 (94.81%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEKFEVVEGKVDWK R AFKHKHGGTRSS LILVAFGFEN+A FALAVNLITYFN+VMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNY+G GYI+SILMA+LADT+LGR KTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKP PCNMFDKQ+HCETV GGNAA LYVALY LAIG+AGIKAALPSH ADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           +QMSSFFN LLLGVC GGA SLTLIVWIQDYKGWDWGLG+SSAAIFF VVIF AG+PLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHI+S SSAIVQILQVY AAIRNRNLVLPEDSADLYE  RDKEA+ EEDFLPHRNIYRFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQQTP GQVETPE S+PWKLCRVTQVENAK+ILSMVPIFCCTIVMTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKLCRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSFHIPPASL IIPVSFIILIVPIYD+IFVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSS+SMAVAALME KRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPK+LKSVSTCFLWSSMALGYFLSTIVVK+VNS TKGITS+GGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITSNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNH 578
           NNINRNHLNLFYWLLSILS INF IY+ VAKRYKYRNH
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYISVAKRYKYRNH 578

BLAST of MC01g1503 vs. NCBI nr
Match: XP_023518209.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1036 bits (2679), Expect = 0.0
Identity = 526/578 (91.00%), Postives = 548/578 (94.81%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEKFEVVEGKVDWK R AFKHKHGGTRSS LILVAFGFEN+A FALAVNLITYFN+VMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNY+G GYI+SILMA+LADT+LGR KTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKP PCNMFDKQ+HCETV GGNAA LYVALY LAIG+AGIKAALPSH ADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           +QMSSFFN LLLGVC GGA SLTLIVWIQDYKGWDWGLG+SSAAIFF VVIF AG+PLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHI+S SSAIVQILQVY AAIRNRNLVLPEDSADLYE  RDKEA+ EEDFLPHRNIYRFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQQTP GQVETPE S+PWKLCRVTQVENAK+ILSMVPIFCCTIVMTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKLCRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSFHIPPASL IIPVSFIILIVPIYD++FVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKLFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSS+SMAVAALME KRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPK+LKSVSTCFLWSSMALGYFLSTIVVK+VNS TKGITS+GGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITSNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNH 578
           NNINRNHLNLFYWLLSILS INF IY+ VAKRYKYRNH
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYISVAKRYKYRNH 578

BLAST of MC01g1503 vs. NCBI nr
Match: XP_022926310.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata])

HSP 1 Score: 1033 bits (2671), Expect = 0.0
Identity = 524/578 (90.66%), Postives = 547/578 (94.64%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEKFEVVEGKVDWK R AFKHKHGGTRSS LILVAFGFEN+A FALAVNLITYFN+VMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNY+G GYI+SILMA+LADT+LGR KTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKP PCNMFDKQ+HCETV GGNAA LYVALY LAIG+AGIKAALPSH ADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           +QMSSFFN LLLGVC GGA SLTLIVWIQDYKGWDWGLG+SSAAIFF VVIF AG+PLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHI+S SSAIVQILQVY AAIRNRNLVLPEDSADLYE  RDKEA+ EEDFLPHRNIYRFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQQTP GQVETPE S+PWK+CRVTQVENAK+ILSMVPIFCCTIVMTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKICRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSFHIPPASL IIPVSFIILIVPIYD++FVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKLFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSS+SMAVAALME KRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPK+LKSVSTCFLWSSMALGYFLSTIVVK+VNS TKGIT +GGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITRNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNH 578
           NNINRNHLNLFYWLLSILS INF IY+ VAKRYKYRNH
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYVSVAKRYKYRNH 578

BLAST of MC01g1503 vs. NCBI nr
Match: XP_008439704.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucumis melo])

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 527/588 (89.63%), Postives = 553/588 (94.05%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEK EVVEGKVDWK R A+KHKHGGTRSS LILVAFGFE++ANFALAVNLITYFNSVMH
Sbjct: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNY+GTGYI+SILMAILADTY GRVKTVIIS C EFLALILLMIQAHYP 
Sbjct: 61  FELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPN 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKPPPCNMFDKQSHCETVEG +AA+LYVALYILAIG AGIKAALPSH ADQFDEKDPKEA
Sbjct: 121 LKPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           MQMSSFFN LLLG+CLGGAVSLTLIVWIQDYKGWDWGLGVSSAA+FFSVVIFVAG+PLYR
Sbjct: 181 MQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHI+SGSS I+QILQVY AAIRNR L+LPEDS  LYE  RDKE A E+DFLPHRNIYRFL
Sbjct: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSTQLYEIERDKEVAAEDDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQ+TPS QVE PEAS+ WKLC VTQVENAKI+LSMVPIFCCTI+MTLCLAQLQTFS
Sbjct: 301 DKAAIQETPSRQVENPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSF IPPASLPIIPVSFIILIVPIYD+I VPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIVPIYDKILVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSS+SMAVAAL+EVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVK+VN  T+GIT SGGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADN 588
           NNINRNHLNLFYW+LSILSLINF IY+FVAK+YKYRNH KP IS A N
Sbjct: 541 NNINRNHLNLFYWMLSILSLINFFIYIFVAKKYKYRNH-KPGISKAVN 587

BLAST of MC01g1503 vs. ExPASy TrEMBL
Match: A0A6J1CLK1 (protein NRT1/ PTR FAMILY 4.6-like OS=Momordica charantia OX=3673 GN=LOC111012279 PE=3 SV=1)

HSP 1 Score: 1149 bits (2973), Expect = 0.0
Identity = 591/591 (100.00%), Postives = 591/591 (100.00%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH
Sbjct: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA
Sbjct: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR
Sbjct: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL
Sbjct: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADNRKP 591
           NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADNRKP
Sbjct: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADNRKP 591

BLAST of MC01g1503 vs. ExPASy TrEMBL
Match: A0A6J1KVN2 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496854 PE=3 SV=1)

HSP 1 Score: 1037 bits (2681), Expect = 0.0
Identity = 527/578 (91.18%), Postives = 548/578 (94.81%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEKFEVVEGKVDWK R AFKHKHGGTRSS LILVAFGFEN+A FALAVNLITYFN+VMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNY+G GYI+SILMA+LADT+LGR KTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKP PCNMFDKQ+HCETV GGNAA LYVALY LAIG+AGIKAALPSH ADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           +QMSSFFN LLLGVC GGA SLTLIVWIQDYKGWDWGLG+SSAAIFF VVIF AG+PLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHI+S SSAIVQILQVY AAIRNRNLVLPEDSADLYE  RDKEA+ EEDFLPHRNIYRFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQQTP GQVETPE S+PWKLCRVTQVENAK+ILSMVPIFCCTIVMTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKLCRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSFHIPPASL IIPVSFIILIVPIYD+IFVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSS+SMAVAALME KRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPK+LKSVSTCFLWSSMALGYFLSTIVVK+VNS TKGITS+GGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITSNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNH 578
           NNINRNHLNLFYWLLSILS INF IY+ VAKRYKYRNH
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYISVAKRYKYRNH 578

BLAST of MC01g1503 vs. ExPASy TrEMBL
Match: A0A6J1EKR5 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111433503 PE=3 SV=1)

HSP 1 Score: 1033 bits (2671), Expect = 0.0
Identity = 524/578 (90.66%), Postives = 547/578 (94.64%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEKFEVVEGKVDWK R AFKHKHGGTRSS LILVAFGFEN+A FALAVNLITYFN+VMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNY+G GYI+SILMA+LADT+LGR KTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKP PCNMFDKQ+HCETV GGNAA LYVALY LAIG+AGIKAALPSH ADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           +QMSSFFN LLLGVC GGA SLTLIVWIQDYKGWDWGLG+SSAAIFF VVIF AG+PLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHI+S SSAIVQILQVY AAIRNRNLVLPEDSADLYE  RDKEA+ EEDFLPHRNIYRFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQQTP GQVETPE S+PWK+CRVTQVENAK+ILSMVPIFCCTIVMTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKICRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSFHIPPASL IIPVSFIILIVPIYD++FVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKLFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSS+SMAVAALME KRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPK+LKSVSTCFLWSSMALGYFLSTIVVK+VNS TKGIT +GGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITRNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNH 578
           NNINRNHLNLFYWLLSILS INF IY+ VAKRYKYRNH
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYVSVAKRYKYRNH 578

BLAST of MC01g1503 vs. ExPASy TrEMBL
Match: A0A1S3AZE0 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484420 PE=3 SV=1)

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 527/588 (89.63%), Postives = 553/588 (94.05%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           MQEK EVVEGKVDWK R A+KHKHGGTRSS LILVAFGFE++ANFALAVNLITYFNSVMH
Sbjct: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMH 60

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNY+GTGYI+SILMAILADTY GRVKTVIIS C EFLALILLMIQAHYP 
Sbjct: 61  FELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPN 120

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           LKPPPCNMFDKQSHCETVEG +AA+LYVALYILAIG AGIKAALPSH ADQFDEKDPKEA
Sbjct: 121 LKPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
           MQMSSFFN LLLG+CLGGAVSLTLIVWIQDYKGWDWGLGVSSAA+FFSVVIFVAG+PLYR
Sbjct: 181 MQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYR 240

Query: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300
           MHI+SGSS I+QILQVY AAIRNR L+LPEDS  LYE  RDKE A E+DFLPHRNIYRFL
Sbjct: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSTQLYEIERDKEVAAEDDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360
           DKAAIQ+TPS QVE PEAS+ WKLC VTQVENAKI+LSMVPIFCCTI+MTLCLAQLQTFS
Sbjct: 301 DKAAIQETPSRQVENPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSF IPPASLPIIPVSFIILIVPIYD+I VPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIVPIYDKILVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSS+SMAVAAL+EVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540
           FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVK+VN  T+GIT SGGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADN 588
           NNINRNHLNLFYW+LSILSLINF IY+FVAK+YKYRNH KP IS A N
Sbjct: 541 NNINRNHLNLFYWMLSILSLINFFIYIFVAKKYKYRNH-KPGISKAVN 587

BLAST of MC01g1503 vs. ExPASy TrEMBL
Match: A0A5D3CMU7 (Protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008480 PE=3 SV=1)

HSP 1 Score: 1028 bits (2658), Expect = 0.0
Identity = 524/586 (89.42%), Postives = 551/586 (94.03%), Query Frame = 0

Query: 3   EKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFE 62
           EK EVVEGKVDWK R A+KHKHGGTRSS LILVAFGFE++ANFALAVNLITYFNSVMHFE
Sbjct: 2   EKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHFE 61

Query: 63  LADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLK 122
           LADAANQLTNY+GTGYI+SILMAILADTY GRVKTVIIS C EFLALILLMIQAHYP LK
Sbjct: 62  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNLK 121

Query: 123 PPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQ 182
           PPPCNMFDKQSHCETVEG +AA+LYVALYILAIG AGIKAALPSH ADQFDEKDPKEAMQ
Sbjct: 122 PPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAMQ 181

Query: 183 MSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMH 242
           MSSFFN LLLG+CLGGAVSLTLIVWIQDYKGWDWGLGVSSAA+FFSVVIFVAG+PLYRMH
Sbjct: 182 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRMH 241

Query: 243 IISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDK 302
           I+SGSS I+QILQVY AAIRNR L+LPEDS  LYE  RDKE A E+DFLPHRNIYRFLDK
Sbjct: 242 IVSGSSTILQILQVYVAAIRNRKLILPEDSTQLYEIERDKEVAAEDDFLPHRNIYRFLDK 301

Query: 303 AAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQ 362
           AAIQ+TPS QVE PEAS+ WKLC VTQVENAKI+LSMVPIFCCTI+MTLCLAQLQTFSIQ
Sbjct: 302 AAIQETPSRQVENPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQ 361

Query: 363 QGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVG 422
           QGLTMDTKLTNSF IPPASLPIIPVSFIILIVPIYD+IFVPF RKLTGIPTGITHLQRVG
Sbjct: 362 QGLTMDTKLTNSFSIPPASLPIIPVSFIILIVPIYDKIFVPFTRKLTGIPTGITHLQRVG 421

Query: 423 VGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFT 482
           VGLVLSS+SMAVAAL+EVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFT
Sbjct: 422 VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFT 481

Query: 483 YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIGNN 542
           YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVK+VN  T+GIT SGGWLIGNN
Sbjct: 482 YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLIGNN 541

Query: 543 INRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDADN 588
           INRNHLNLFYW+LSILS+INF IY+FVAK+YKYRNH KP IS A N
Sbjct: 542 INRNHLNLFYWMLSILSMINFFIYIFVAKKYKYRNH-KPGISKAVN 586

BLAST of MC01g1503 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 466.1 bits (1198), Expect = 4.3e-131
Identity = 258/598 (43.14%), Postives = 379/598 (63.38%), Query Frame = 0

Query: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60
           ++E+    EG  DW+ R+A K +HGG  ++  +LV    ENLA  A A NL+ Y    MH
Sbjct: 3   VEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMH 62

Query: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120
              + +AN +TN++GT +++++L   L+D +    +  +IS  +EFL LI+L IQA  P 
Sbjct: 63  MSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPS 122

Query: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180
           L PP C+       CE V G  AA+L+V LY++A+G  GIK +L SH A+QFDE  PK  
Sbjct: 123 LMPPSCD----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGR 182

Query: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240
            Q S+FFN+ +  +  G  V++T +VW++D KGW+WG GVS+ AIF S++IF++G   YR
Sbjct: 183 KQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYR 242

Query: 241 MHIISGS--SAIVQIL---------------QVYGAAIRNRNLVLPEDSADLYETVRDKE 300
             I  GS  + I+++L                V   ++   N  + +   ++ E+  + E
Sbjct: 243 NKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEV-ESQGELE 302

Query: 301 AATEEDFLPHR----NIYRFLDKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSM 360
              +E+ LP R    N  + L+ AA ++     +E          C V QVE+ KI+L M
Sbjct: 303 KPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLE----------CTVQQVEDVKIVLKM 362

Query: 361 VPIFCCTIVMTLCLAQLQTFSIQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDR 420
           +PIF CTI++  CLAQL TFS+QQ  +M+TK+  S  IPPASLPI PV FI+++ PIYD 
Sbjct: 363 LPIFACTIMLNCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDH 422

Query: 421 IFVPFARKLTGIPTGITHLQRVGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQ 480
           + +PFARK T   TG+THLQR+GVGLVLS ++MAVAAL+E+KRKGVA+D  +LD+    +
Sbjct: 423 LIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDS---KE 482

Query: 481 PLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI 540
            LP++  W++ Q+   G ADLFT  GLLE+F++EAP +++S++T   W+S+A+GY+LS++
Sbjct: 483 TLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSV 542

Query: 541 VVKVVNSVTKGITSSGGWLIGNNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRN 578
           +V +VNS+T G + +  WL G +INR  L+ FYWL+ +LS  NF  YLF A RYKYR+
Sbjct: 543 IVSIVNSIT-GSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 580

BLAST of MC01g1503 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 451.8 bits (1161), Expect = 8.3e-127
Identity = 248/567 (43.74%), Postives = 357/567 (62.96%), Query Frame = 0

Query: 9   EGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAAN 68
           EG  DW+ ++A + +HGG  ++  +L     ENLA  A A NL+ Y  + MH  LA +++
Sbjct: 9   EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 68

Query: 69  QLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNM 128
           ++T ++ T +++++L   LAD +       +IS  +EFL LILL IQA  P L PPPC  
Sbjct: 69  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS 128

Query: 129 FDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFN 188
                 CE V G  AA L+V LY++++G  GIK +LPSH A+QFDE  PK   Q S+FFN
Sbjct: 129 -SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 188

Query: 189 HLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSS 248
           + +  +  G  V++T +VWI+D KGW+WG GVS+ +IF S+++F+ G   Y+  I  GS 
Sbjct: 189 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 248

Query: 249 AIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDKAAIQQT 308
                  +  A+I + +     +     E   + E  T    L   N    L+KA   +T
Sbjct: 249 LTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL--TNSLTCLNKAIEGKT 308

Query: 309 PSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMD 368
                      + W  C V QVE+ KI+L M+PIF CTI++  CLAQL T+S+ Q  TM+
Sbjct: 309 ----------HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMN 368

Query: 369 TKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVGVGLVLS 428
            K+ N F++P ASLP+ PV F++++ P YD + +PFARK+T    GITHLQR+GVGLVLS
Sbjct: 369 RKIVN-FNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLS 428

Query: 429 SMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLE 488
            ++MAVAAL+E+KRK VAR+  +LD+    + LPI+  W++ Q+   G ADLFT  GLLE
Sbjct: 429 IVAMAVAALVELKRKQVAREAGLLDS---EETLPITFLWIALQYLFLGSADLFTLAGLLE 488

Query: 489 FFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIGNNINRNHL 548
           FF++EAP +++S++T   W+S+ALGY+LS+++V +VN VTK    S  WL G  +NRN L
Sbjct: 489 FFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQS-PWL-GEKLNRNRL 548

Query: 549 NLFYWLLSILSLINFSIYLFVAKRYKY 576
           +LFYWL+ +LS++NF  YLF AKRYKY
Sbjct: 549 DLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of MC01g1503 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 451.8 bits (1161), Expect = 8.3e-127
Identity = 248/567 (43.74%), Postives = 357/567 (62.96%), Query Frame = 0

Query: 9   EGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAAN 68
           EG  DW+ ++A + +HGG  ++  +L     ENLA  A A NL+ Y  + MH  LA +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 69  QLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNM 128
           ++T ++ T +++++L   LAD +       +IS  +EFL LILL IQA  P L PPPC  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS 132

Query: 129 FDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFN 188
                 CE V G  AA L+V LY++++G  GIK +LPSH A+QFDE  PK   Q S+FFN
Sbjct: 133 -SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 189 HLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSS 248
           + +  +  G  V++T +VWI+D KGW+WG GVS+ +IF S+++F+ G   Y+  I  GS 
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 252

Query: 249 AIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDKAAIQQT 308
                  +  A+I + +     +     E   + E  T    L   N    L+KA   +T
Sbjct: 253 LTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL--TNSLTCLNKAIEGKT 312

Query: 309 PSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMD 368
                      + W  C V QVE+ KI+L M+PIF CTI++  CLAQL T+S+ Q  TM+
Sbjct: 313 ----------HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMN 372

Query: 369 TKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVGVGLVLS 428
            K+ N F++P ASLP+ PV F++++ P YD + +PFARK+T    GITHLQR+GVGLVLS
Sbjct: 373 RKIVN-FNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLS 432

Query: 429 SMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLE 488
            ++MAVAAL+E+KRK VAR+  +LD+    + LPI+  W++ Q+   G ADLFT  GLLE
Sbjct: 433 IVAMAVAALVELKRKQVAREAGLLDS---EETLPITFLWIALQYLFLGSADLFTLAGLLE 492

Query: 489 FFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIGNNINRNHL 548
           FF++EAP +++S++T   W+S+ALGY+LS+++V +VN VTK    S  WL G  +NRN L
Sbjct: 493 FFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQS-PWL-GEKLNRNRL 552

Query: 549 NLFYWLLSILSLINFSIYLFVAKRYKY 576
           +LFYWL+ +LS++NF  YLF AKRYKY
Sbjct: 553 DLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of MC01g1503 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 432.6 bits (1111), Expect = 5.2e-121
Identity = 243/560 (43.39%), Postives = 348/560 (62.14%), Query Frame = 0

Query: 9   EGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAAN 68
           E  VDW+ R +  +KHGG R++  +L    FE +   A+  NLITY  + MHF L+ AAN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 69  QLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNM 128
            +TN++GT +I ++L   L+D +LG   T+II G +E    ILL +QAH P+LKPP CN 
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 129 FDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFN 188
              Q+ CE  +G  A + ++ALY++A+G+  +K  + +H ADQF +  PK++ ++SS+FN
Sbjct: 147 LIDQT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFN 206

Query: 189 HLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSS 248
                  +G  ++LTL+VW+Q + G D G GVS+AA+   ++  V+G  +Y  +     S
Sbjct: 207 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSG-TMYFRNKRPRRS 266

Query: 249 AIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDKAAIQQT 308
               I  V  AAI  R L  P D   L+              LPH   +RFLDKA I+  
Sbjct: 267 IFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQ 326

Query: 309 PSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMD 368
                +T    +PW+LC VTQVE  K ++S+VPIF  TIV    LAQLQTFS+QQG +M+
Sbjct: 327 -----DTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 386

Query: 369 TKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVGVGLVLS 428
           T+L+NSFHIPPASL  IP   +I +VP+YD   VPFARKLTG  +GI  L R+G+GL LS
Sbjct: 387 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 446

Query: 429 SMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLE 488
           + SM  AA++E KR    RD ++LD         +S FW++ QF IFGI+++FT VGL+E
Sbjct: 447 TFSMVSAAMLEKKR----RDSSVLDGR------ILSIFWITPQFLIFGISEMFTAVGLIE 506

Query: 489 FFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIGNNINRNHL 548
           FFY ++ K ++S      + S + G++ S+++V VVN +T     S GWL  N++N++ L
Sbjct: 507 FFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRL 566

Query: 549 NLFYWLLSILSLINFSIYLF 569
           +LFYWLL++LSL+NF  YLF
Sbjct: 567 DLFYWLLAVLSLLNFLSYLF 569

BLAST of MC01g1503 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 416.4 bits (1069), Expect = 3.9e-116
Identity = 236/564 (41.84%), Postives = 347/564 (61.52%), Query Frame = 0

Query: 12  VDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFELADAANQLT 71
           VD++ R     KHGGTR++  +L    FE +A  A+  NLITY  + MHF L+ +AN +T
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 72  NYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPPCNMFDK 131
           N++GT +++S+L   L+D+YLG  +T+++ G +E    ILL +QAH P+L+PP CNM   
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 132 QSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQMSSFFNHLL 191
             HC    G  AA LY AL ++A+G+  +K  + SH A+QF  KD +   ++SSFFN   
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKDLR---KLSSFFNAAY 206

Query: 192 LGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMHIISGSSAIV 251
               +G  ++LTL+VW+Q + G D G GVS+A +   ++  VAG   YR    SG S   
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSG-SIFT 266

Query: 252 QILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFLDKAAIQQTPSG 311
            I QV+ AAI  R  + P +   +++   D         L H N +RFLDKA I      
Sbjct: 267 PIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKP---LLHSNKFRFLDKACI------ 326

Query: 312 QVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMDTKL 371
           + +     +PW+LC + QV   KI+LS++PIF CTI+    LAQLQTFS+QQG +M+T +
Sbjct: 327 KTQGKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 386

Query: 372 TNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQRVGVGLVLSSMS 431
           T +F IPPASL  IP   +I  VP+Y+  FVP ARKLTG  +GI+ LQR+G GL L++ S
Sbjct: 387 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 446

Query: 432 MAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFY 491
           M  AAL+E KR+    + N++          +S FW++ QF IFG++++FT VGL+EFFY
Sbjct: 447 MVAAALVEKKRRESFLEQNVM----------LSIFWIAPQFLIFGLSEMFTAVGLVEFFY 506

Query: 492 SEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVT--KGITSSGGWLIGNNINRNHLN 551
            ++ ++++S  T   + S + G++LS+++V  VN VT   G  +  GWL  N++N++ L+
Sbjct: 507 KQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLD 566

Query: 552 LFYWLLSILSLINFSIYLFVAKRY 574
            FYWLL+ LS INF  YLF ++ Y
Sbjct: 567 HFYWLLASLSFINFFNYLFWSRWY 567

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1576.0e-13043.14Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE41.2e-12543.74Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q93VV57.3e-12043.39Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q56XQ65.4e-11541.84Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q9FM202.6e-10940.00Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_022142057.10.0100.00protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia][more]
XP_023003153.10.091.18protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima][more]
XP_023518209.10.091.00protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo][more]
XP_022926310.10.090.66protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata][more]
XP_008439704.10.089.63PREDICTED: protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1CLK10.0100.00protein NRT1/ PTR FAMILY 4.6-like OS=Momordica charantia OX=3673 GN=LOC111012279... [more]
A0A6J1KVN20.091.18protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496854 PE... [more]
A0A6J1EKR50.090.66protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111433503 ... [more]
A0A1S3AZE00.089.63protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A5D3CMU70.089.42Protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
AT1G69850.14.3e-13143.14nitrate transporter 1:2 [more]
AT1G27040.28.3e-12743.74Major facilitator superfamily protein [more]
AT1G27040.18.3e-12743.74Major facilitator superfamily protein [more]
AT1G59740.15.2e-12143.39Major facilitator superfamily protein [more]
AT1G33440.13.9e-11641.84Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 96..529
e-value: 2.0E-83
score: 280.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 11..581
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..582
e-value: 2.9E-163
score: 545.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 28..578
NoneNo IPR availablePANTHERPTHR11654:SF336PROTEIN NRT1/ PTR FAMILY 4.2coord: 11..581

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g1503.1MC01g1503.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042981 regulation of apoptotic process
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity